DNA (. 9;. ( ) 66) : [ ] DNA [ ] 7 9 6 DNA DNA [ ] 8 DNA 8.%99.9%; 7.%(9/)99.%(/); 9.7%(/) 99.9% [ ] DNA : DNA ; ; :R77. :A :-()7-9- S tu dy of th e Applica tion of DNA-im a ge -cytom e try (DNA-ICM) a n d Cytology for Ce rvica l Ca n ce r S cre e n in g SHEN Nia n-c hun ZHU C he n-ya n ZHANG He e t a l. (.Fa mily Pla nning Se rvic e Ce nte r of Zhuha izhuha i 9 China ;. Motic (Xia m e n ) Me d - ic a l Dia g nostic Ce nte rxia me n 66 China ) Abstract: [Purpose ] To investigate the value of DNA-image-cytometry (DNA-ICM) and conventional cytology in the cervical cancer screening. [Methods ] Two thousand one hundred and six women undergoing cervical cytological examination in Zhuhai Family Planning Service Center during Jan. 7 to Dec. 9 were enrolled in the study. Every sample was detected by both DNA-ICM and conventional cytology. Biopsy was taken if positive result was found and pathology of colposcopic biopsy was considered as golden standard. The relative sensitivity relative specificity positive predictive value (PPV) and negative predictive value (NPV) of DNA-ICM conventional cytology and combining both of them were calculated. [Results ] Two thousand and eighty-three samples were satisfactory. Of the cases recommended for biopsy had pathological diagnosis and follow-up. Relative sensitivity and relative specificity were 8.% and 99.9% of DNA-ICM; 7.% and 99.% of coventional cytology; 9.7% and 99.% combination of the two tests respectively. [Conclusion] Being compared with conventional cytology DNA-ICM has the higher sensitivity on detection of the cervical cancer and pre-cancerous lesions with no less specificity. If DNA-ICM was combined with conventional cytology the sensitivity of the test will be improved. Key words: DNA cytometry; cytology ; cervical cancer; screening : :--; :--8 : (97) : E-mail: julia8@hotmail.com [] [] 7 China CancerVol.No.7 9
. 7 9 / (intergrated optical density 9~ IOD) IOD 68 7.7. DI. ( %~% )~6 DI. % [] : (DI.) DNA DNA Feulgen DI. DNA DNA. : DNA : DNA Feulgen MotiCytometer (NCI) TBS (The Bethesda (System) (within normal limitswnl) ) (atypical squamous cells of unde- significanceasc-us) DNA terminied (atypical squamous cell-can- MotiCytometer not exclude HSILASC-H) 8 (low grade squa- mous intraepithelical lesion LSIL) (high grade squamous intraepithelical lesionhsil) (squa- mous cell carcinomascc) (atypical DNA (DNA indexdi) glandular cells of undetermined- : DNA significaneagus) (adeno- (DI=±.) carcinomaac) (.<DI<.) : (DI.) MotiCytometer TBS ASC-H LSIL DNA HSIL/CIS SCCAGUS AC;DNA : DI. ~ DI. % ; ( % DNA ) ~ : 7 China CancerVol.No.7
DNA % 8.6% TBS : DNA 7.% CIN CIN CIN(ROC.9() 99.% ) CIN. 9% 7.% 78.6% TBS. (P=.). TBS TBS TBS ROC ROC ROC.87() SPSS 6. DNA TBS ASC-H ASCUS NILM 6 DNA + /- DNA TBS CIN) (. DNA 8 DNA ;. DNA.8 DNA.6 ASCUS DNA ~. DNA. DNA DNA 8.% 99.% 8 DNA CIN/CIS CIN CIN + 9 /- 6 CIN/CIS CIN CIN. DNA +TBS + /- TBS + /- 9 9 8 6...6.8. - DNA ROC 7 China CancerVol.No.7
DNA 9.%. 9% 8% ROC DNA+TBS (P =.9 ( ) DNA + /-.) TBS CIN/CIS CIN CIN 8.. 9% (P=.) 9.7% 99.%..8.6....8.6.....6.8. - TBS ROC...6.8. - DNA+TBS ROC %~% [] [6] DNA [7] DNA [8~] DNA DNA ASC-H DNA ( 7 China CancerVol.No.7
and accuracy in liquid-based versus ) conventional cytology: a systematic review[j]. Lancet 6 67(): -. TBS [] Solomon D DaveyD Kurman R CIN et al. The Bethesda System: terminology for reporting results of cervical 7.%(9/) cytology[j]. JAMA 87(6): - DNA 9. 8.% [] Remmerbach T Weidenbach H Pomjanski N et al. Cytologic and DNAcytometric ( 99.%) early diagnosis of oral cancer DNA [J]. Anal Cell Pathol l (): -. DNA [6] Obwegeser J Schneider V. Thinlayer cervical cytology: a new meta-anal- ysis[j]. Lancet 6 67(): 88-89. [7] Sudbo J Reith A. The evolution of ROC predictive oncology and molecular-based therapy for oral cancer prevention[j]. Int J Cancer : 9-. DNA [8] Azúa J Romeo P Morales M et al. DNA quantification as a prognostic factor in gastric adenocarcinoma [J]. Anal : TBS Quant Cytol Histol 998():-. DNA []. (ACCP) [9] Dey P Luthra UK Prasad A et al. TBS Cytologic grading and DNA image cytomelry of breast carcinoma on fine nee- DNA [J]. 9 69(): 7. dle aspiration cytology smear [J]. Anal [] Benedet JL Anderson GH. Cervical Quant Cytol Histol 999 (): 7-. intraepithelial neoplasia (9.7% ) in British [] Anderson G MacAulay CF Matisic J et al. The use of an automated Columbia: a comprehensive program for (99.%) ROC detection diagnosis and treatment [J]. image cytometer for screening and quantitative assessment of cervical lesions for Gynecol Oncol 98 : s8-9. [] Davey E Barratt A Irwig L et al. screening. Columbia Cervical Smear Effect of study design and quality on unsatisfactory rates cytology classification Screening Programme [J]. Cytopathology 997 8(): 98-. 7 China CancerVol.No.7