BioProcess Systems. Choose your standard. Courtesy of Avecia Ltd. UK.

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BioProcess Systems Choose your standard Courtesy of Avecia Ltd. UK. BioProcess chromatography systems from process development to full-scale production 18-1150-34 Edition AD

BioProcess over 1200 Column protection: Bubble trap with bypass valves. Optional automatic vent valve and level sensor for cleaning purposes. Pre-column air sensor protects the column from air bubbles. Pre-column conductivity monitor: Closed-loop gradient control for accurate and reproducible gradients. Flow-through pressure monitor: Protects the system and column from overpressure. Triple-headed diaphragm pumps: Reliable low-pulsation displacement pump, with multi-layer diaphragms. Robust and scalable two-pump configuration for accurate and reproducible gradients. Single pump setup for isocratic or step-gradient production runs also available. As well as our range we offer customized an to meet your

systems worldwide Transmitters: Post column in-line monitoring of flow, conductivity, ph and UV provides important data for analysing and controlling your process. Mass flow meter: Clean flow-through design with no moving parts. Precise measurements independent of fluid characteristics. Valves: Multi-port or zero dead-leg diaphragm valves reduce system hold-up volume. of standard systems, d engineered solutions specific needs.

Standard design. Available in three piping dimensions: 3, 6, or 10 mm. Polypropylene system tubing. Sanitary components and fittings throughout. Four-layer sandwich diaphragms used in pump heads. Air sensor. Flow-through pressure monitor. Flow-through conductivity monitor. Clear labelling of all system components. Complete system documentation. Validatable process control system with validation packages. UNICORN control. Features Direct scale up from ÄKTA system. Allows fast delivery. Fits through most standard doorways. Easy access for maintenance and service. Various flow ranges 2 30, 7.5 120, 27 400 l/h. Chemically resistant, compatible with solutions commonly used in process chromatography. Easy to clean. Benefits Provides multiple-level safety for product, robust system. Easy to adjust sensitivity. Consistent performance irrespective of fluid characteristics. Sanitary design, no T-connection. Sanitary straight design, easily cleaned, reduces hold-up volume. Easily identifiable components facilitate validation procedures. Speeds up validation processes. Efficient validation. IQ/OQ packages services available. Allows connection of multiple systems to one computer. One control station lowers costs and simplifies system set-up. Communicates with OPC clients. Same software package, reproducible results.

Gradient systems 100% 90% 80% 70% Gradient Range 60% 50% 40% 30% 20% 10% 0% 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Flow Rate (as a fraction of the system s maximum rating) At factory settings, these BioProcess systems are capable of developing gradients within the shaded area shown. The pumps can be adjusted to produce wider gradient ranges at lower flow rates. Resulting gradient Programmed gradient Performance test of step and linear gradients. UNICORN control Inlet 3 Inlet 1 001 Inlet 2 Sample AS 151 Inlet 6 Inlet 4 003 Inlet 5 Inlet 7 002 004 201.A 201.B 021 211 221 022 031 033 Column 032 051 1 2 AA 152 3 4 5 6 1 PIA111 bar 4 CIR102 ms/cm 2 CIC101 ms/cm 5 AIR121 ph 3 FIC141 l/h 6 AIR131 AU Outlet 2 Waste 052 Outlet 1 Outlet 3 Active flow path is illuminated and data are displayed in real time. BioProcess systems are controlled by UNICORN. This user-friendly control system is designed for real-time control and monitoring of chromatography systems and includes a PC-based man machine interface.

System specifications 3 mm system 6 mm system 10 mm system Flow range (l/h)* 2 30 7.5 120 27 400 Maximum operating pressure (bar) 3 3 3 Number of inlets Isocratic/ Gradient system 6/6 6/8 6/8 Number of outlets 4 4 4 Temperature range ( C) 4 40 4 40 4 40 Dimensions (WxLxH) (mm) 776x1400x1895 820x1400x1897 860x1400x1897 Power requirement 110/220 VAC, 50/60 Hz 110/220 VAC, 50/60 Hz 110/220 VAC, 50/60 Hz Compressed air requirement min/max (bar) 6/10 6/10 6/10 Measuring range Conductivity (ms/cm) 0 200 0 200 0 200 ph 0 14 0 14 0 14 UV (AU) 0 4 0 4 0 4 * Lower flow rates may be achieved with adjustment of pump stroke lengths Ordering information Services Options Code Additional Code Automatic Code Additional Code System number Inlets number Vent valve number Outlets number 3-mm Isocratic system 18-1148-23 4 18-1150-56 Yes 18-1150-63 3 18-1150-57 3-mm Gradient system 18-1148-22 Yes 18-1150-63 3 18-1150-57 6-mm Isocratic system 18-1148-25 4 18-1150-58 Yes 18-1150-64 4 18-1150-59 6-mm Gradient system 18-1148-24 Yes 18-1150-64 4 18-1150-59 10-mm Isocratic system 18-1148-27 4 18-1150-61 Yes 18-1150-65 4 18-1150-62 10-mm Gradient system 18-1148-26 Yes 18-1150-65 4 18-1150-62 Accessories include: filter housing; cleaning-in-place manifold; tubing; clamps and gaskets. Service Agreement Fast Trak Services Different types of service agreements are available to cover your needs and your budget. Customized features such as specific response time, warranty extension, additional calibration, preventive maintenance visits, gasket exchange can be included as required. Please contact your local representative for more information. Fast Trak Education is a consulting and educational service that provides practical and advisory support from startup to routine production. Hands-on training courses and seminars cover chromatographic techniques, column packing, optimization, scale-up and validation. Courses can be customized to your needs. Fast Trak Validation is a consulting service to assist you with designing and validating your downstream purification processes. Regulatory support documentation includes Validation Support Packages for UNICORN software; Installation & Operational Qualification (IQ/OQ) documentation for systems and columns; Change Control Procedure documentation. We can also perform the IQ/OQ for your company. www.bioprocess.amershambiosciences.com Asia Pacific Tel: +852 2811 8693 Fax: +852 2811 5251 Australasia Tel: +61 2 9899 0999 Fax: +61 2 9899 7511 Austria Tel: 01 576 0616 20 Fax: 01 576 0616 27 Belgium Tel: 0800 73 888 Fax: 03 272 1637 Canada Tel: 1 800 463 5800 Fax: 1 800 567 1008 Central, East, South East Europe Tel: +43 1 982 3826 Fax: +43 1 985 8327 Denmark Tel: 45 16 2400 Fax: 45 16 2424 Finland & Baltics Tel: +358 (0)9 512 3940 Fax: +358 (0)9 512 1710 France Tel: 0169 35 67 00 Fax: 0169 41 9677 Germany Tel: 0761 4903 401 Fax: 0761 4903 405 Italy Tel: 02 27322 1 Fax: 02 27302 212 Japan Tel: 81 3 5331 9336 Fax: 81 3 5331 9370 Latin America Tel: +55 11 3933 7300 Fax: +55 11 3933 7306 Middle East and Africa Tel: +30 2 10 96 00 687 Fax: +30 2 10 96 00 693 Netherlands Tel: 0165 580 410 Fax: 0165 580 401 Norway Tel: 2318 5800 Fax: 2318 6800 Portugal Tel: 21 417 7035 Fax: 21 417 3184 Russian & other C.I.S. & N.I.S. Tel: +7 (095) 232 0250,956 1137 Fax: +7 (095) 230 6377 South East Asia Tel: 60 3 8024 2080 Fax: 60 3 8024 2090 Spain Tel: 93 594 49 50 Fax: 93 594 49 55 Sweden Tel: 018 612 19 00 Fax: 018 612 19 10 Switzerland Tel: 01 802 81 50 Fax: 01 802 81 51 UK Tel: 0800 616 928 Fax: 0800 616 927 USA Tel: +1 800 526 3593 Fax: +1 877 295 8102 Amersham Biosciences reserves the right to change/replace system components. (Contact your local representative for information): BioProcess Systems and Drop Design are trademarks of Amersham Biosciences Limited. Amersham and Amersham Biosciences are trademarks of Amersham plc. All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group that supplies them. A copy of these terms and conditions is available on request. Amersham Biosciences UK Limited, Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA, England. Amersham Biosciences AB, Björkgatan 30, SE-751 84 Uppsala, Sweden. Amersham Biosciences Corp., 800 Centennial Avenue, PO Box 1327, Piscataway NJ 08855, USA. Amersham Biosciences Europe GmbH, Munzinger Strasse 9, D-79111 Freiburg, Germany. Amersham Biosciences KK, Sanken Bldg. 3-25-1, Hyakunincho Amersham Shinjuku-ku, Tokyo 169-0073, Japan. Amersham Biosciences AB 2004 All rights reserved.