Acidulants in Beverages



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Acidulants in Beverages Daniel Sortwell Bartek Ingredients Inc. Ontario, Canada

How do you know which acidulants to choose? Citric Malic Lactic Fumaric Tartaric Phosphoric Sodium Acid Sulfate

Use qualitative screens to narrow down your selection taste and flavour effects physicochemical properties acid strength

Improve beverage flavour profile by using acidulants

Review taste and flavour effects to determine the result you want Olfactory Membrane Aromatic flavor notes Flavor modification (blending, enhancement) Sourness Astringency ph effects Chemical irritation

Review taste and flavour effects to determine the result you want Sourness at ph 3.0, 0.1% w/v Descriptors Flavor modifier Temporal Citric llllllllllllllllllllllllllllllllllll bright, refreshing Fumaric llllllllllllllllllllllllllllllllllllllllllll clean, dry Lactic lllllllllllllllllllllllllllllllllllllllll smooth, dairy Malic llllllllllllllllllllllllllllllllllllllllllll mellow, smooth Phosphoric lllllllllllllllllllllllllll low impact SAS lllllllllllllllllllllllllll low impact Tartaric lllllllllllllllllllllllllllllllllllll brusque, dry

Mark your sample cups, please C T M A B

Review taste and flavour effects - demo Order of tasting: C - Citric Acid 0.08% w/v T - Tartaric Acid 0.08% w/v M - Malic Acid 0.08% w/v

Acids naturally present in fruit can produce true fruit flavour Tamarind Grape Apple Lychee Peach Pineapple Mango Orange Malic Tartaric Citric Lemon 0 25 50 75 100 % of Total Acid

For example, Malic Acid enhances citrus flavour notes Malic and Citric Acid Levels in Citrus Fruit Juices (% w/v ± std. error) Fruit, Region (variety) Malic Acid Citric Acid sample size, n Lemon Israel 0.26±0.03 5.20±0.33 11 California (eureka) 0.22 4.19 2 Mandarin California (dancy) 0.20 1.04 2 Orange Brazil first press 0.20±0.02 0.90±0.05 58 USA and Cuba 0.16±0.02 0.84±0.13 34 Spain (navelina) 0.13±0.01 1.17±0.06 63 Mediterranean 0.12±0.03 1.15±0.10 15

Malic Acid enhances orange flavour Ingredient Use level (%w/v) Water to 100 Sucrose 12.50 Formula A - 100% Citric Acidulant 0.30 Orange flavour & color 0.07 Formula B - 95/5 Citric/Malic Preferred by 10/14 panelists Sodium Citrate 0.04 Sodium Benzoate 0.03 from "Improving the flavor of fruit products with acidulants" at www.bartek.ca

Malic Acid blends together discordant flavour notes Unblended Blended

Evaluate different levels of Malic Acid with the dropper bottle 10% w/v Malic Acid 1 drop in 200 ml. = 17.5 ppm. 3 drops in 200 ml. = 52.5 ppm.

Higher rated beverages contain Malic Acid (www.bevnet.com) 50 with Malic Acid without Malic Acid % of Beverages in the Stated Rating Group 25 0 horrible mediocre good very good excellent perfect bevnet Beverage Ratings

Narrow the selection further with physicochemical properties Liquid Volatile Liquid Phosphoric Acetic Corrosive Fumaric Citric Malic SAS Tartaric Lactic Irritant Nonhygroscopic solid, low solubility Hygroscopic solid, high solubility Liquid

Log P values predict antimicrobial activity and sourness persistence Log(octanol/water partition coefficient) of undissociated organic acids (Leo et al., 1971) Fumaric 0.28 Acetic -0.31 Lactic -0.62 Malic -1.26 Citric -1.72 Tartaric -2.02

Acid strength determines level required to achieve target ph Acid level required to lower ph of 0.005N NaOH to a specific value (% w/v) 0.4 0.2 MA CA TA FA LA, 80% SAS, 93% PA, 85% LA: Lactic Acid MA: Malic Acid CA: Citric Acid TA: Tartaric Acid FA: Fumaric Acid SAS: Sodium Acid Sulfate PA: Phosphoric Acid 0.0 3.0 3.5 4.0 ph

High sourness results from a low ph with weak acids 12 Lactic Malic Estimated Sourness (units from ratio scales of H.Moskowitz (1971)) 8 4 Citric Fumaric Tartaric Phosphoric (based on limited data) 0 3.0 3.5 4.0 ph I = 0.005

Calcium-fortified beverages are prone to calcium salt precipitation

Use acid blends in fruit flavoured beverages NUT APM APM/ ACE-K SUC SUC/ ACE-K C C C C C CFP CM CM CM CM CL CMT M M CPM CM M TM M CMT MC PCM CP F C = Citric and/or Citrate FC F = Fumaric L L = Lactic M M = Malic MC MPC T = Tartaric P = Phosphoric and/or Phosphate

Take advantage of the sourness additive effect with acid blends Estimated Sourness at ph 3.2, 0.2% total Acid, I = 0.005 7 6 16% increase in sourness from 100/0 to 90/10 0 20 40 60 80 100 100 80 60 40 20 0 % Citric % Malic

Take advantage of the sourness additive effect with acid blends Sample Formulation ph A Estimated Sourness 0.16% Citric Acid 0.04% Sodium Citrate 3.03 5.5 B 0.10% Citric Acid 0.03% Malic Acid 0.04% Sodium Citrate 3.22 5.8

Target ph determines buffer salt level of use Buffer salt Level Required to Achieve ph 3.35 (% w/v)* Sodium Level (% w/v) Est. Total Buffer Capacity (Equiv/L) Calcium Fumarate trihydrate 0.22 ---- 0.0167 Sodium Lactate 0.14 0.029 0.0143 Trisodium Citrate dihydrate 0.12 0.028 0.0113 Trisodium Phosphate 0.07 0.029 0.0095 *Each buffer salt was added to a mixed solution of Citric Acid (0.16% w/v) and Malic Acid (0.05% w/v) paper at: http://ift.confex.com/ift/responses/2004/36.ppt

Increase sourness at a constant ph by raising level of buffer pair ph 3.5 ph 3.0 NaLactate/Malic NaLactate/Malic NaLactate/Lactic 20 NaCitrate/Malic NaCitrate/Malic NaCitrate/Citric Estimated Sourness (Bartek models) 10 NaCitrate/Citric NaLactate/Lactic 0 0.0 0.5 1.0 Concentration of Buffer Pair (% w/v)

Save time by using Bartek s web site: www.bartek.ca

Peach/Mango Fruit Smoothie: Citric/Lactic water, sugar, white grape juice from concentrate, peach puree from concentrate, skim milk, mango puree, pectin, natural flavors, citric acid, cream, lactic acid, rosemary extract, salt bevnet rating: very good

Banana Colada Fruit Smoothie: Lactic/Citric filtered water, crystalline fructose, skim milk, banana puree, apple juice concentrate, natural flavor, sugar, tricalcium phosphate, cream, lactic acid, pectin, citric acid, ascorbic acid, niacin, calcium pantothenate, vitamin e acetate, sucralose, pyridoxine hydrochloride, vitamin a palimate, cyanacobalamin bevnet rating: excellent

Carbonated Energy Drink: Citric/Lactic/Cider Vinegar carbonated water, sugar, kombucha concentrate, citric acid, lactic acid, cider vinegar, guarana extract, vitamin blend (vitamin c, niacin, pantothenic acid, vitamin b6, folic acid), malt extract, ginseng extract, flavors bevnet rating: very good

Non-carbonated Energy Drink: Citric/Malic water, high fructose corn syrup, glucose, natural and artificial flavors, taurine, citric acid, malic acid, d- glucuronolactone, ascorbic acid, inositol, choline, caffeine, niacin (B-3), pantothenic acid (b-5), FD&C yellow #5 and #6, pyridoxine (b6), cyanocobalamin (B-12) bevnet rating: very good

Carbonated grape soft drink: Citric/Tartaric carbonated water, high fructose corn syrup, pure washington honey, gum acacia, maltodextrine, citric acid, sodium benzoate (for freshness), tartaric acid and natural and artificial flavors bevnet rating: excellent

Carbonated cola/tea drink: Citric/Malic/Tartaric carbonated water, sugar, invert sugar, natural flavors, green tea powder, citric acid, malic acid, tartaric acid, vanilla extract, kola nut extract, sodium benzoate and potassium sorbate bevnet rating: excellent

Still white tea drink: Malic water, cane juice, white tea blend, malic acid, ginseng, natural flavor bevnet rating: excellent

Zero calorie sports drink: Citric/Malic water, citric acid, natural punch flavor with other natural flavors, malic acid, salt, monopotassium phosphate, sodium citrate and sucralose bevnet rating: excellent

Sugar free ginger ale: Citric/Malic carbonated water, natural and artificial flavors, citric acid, malic acid, sucralose, sodium benzoate and potassium sorbate, sodium citrate, caramel color bevnet rating: perfect

Low calorie carbonated black raspberry soft drink: Malic carbonated spring water, natural flavors, marion blackberry juice concentrate, malic acid, sucralose, potassium benzoate to ensure freshness, ascorbic acid (vitamin c), vitamin e acetate bevnet rating: excellent

Zero calorie flavoured water: Citric/Malic spring water, citric acid, natural flavor, malic acid, sodium benzoate and potassium sorbate, sucralose bevnet rating: excellent

Low calorie flavoured water: Citric/Malic mineral water, fructose, carbon dioxide, citric acid, malic acid, acesulfame potassium, aspartame, sodium citrate, potassium sorbate, flavor bevnet rating: excellent

Enhanced water (women s health) Citric/Malic water, calcium lactate, cranberry juice concentrate, natural flavors, citric acid, magneseum lactate, malic acid, sucralose, potassium sorbate, acesulfame-k, soy isoflavones, vegetable juice (for color), red 40, liposomes(water, lecithin, vitamin d), pyridoxine, hydrochloride, folic acid, vitamin b12 bevnet rating: excellent

Zero calorie white tea drink: Malic/Citric filtered water, natural pomegranate passionfruit flavor with other natural flavors, white tea solids, malic acid, white tea extract, ascorbic acid, acesulfame potassium, citric acid, sucralose, niacin (B3), calcium pantothenate, vitamin E acetate, pyridoxine hydrochloride (B6), folic acid, cyanocobalamin bevnet rating: excellent

Low calorie vanilla berry hibiscus drink: Malic/Citric filtered water, organic evaporated cane juice, apple and pineapple juices from concentrate, natural flavors, hibiscus extract, vegetable juice for color, malic acid, citric acid bevnet rating: perfect

Improve beverage flavour profile by using acidulants