LABORATORIO DI ENTOMOLOGIA AGRARIA

BOLLETTINO del LABORATORIO DI ENTOMOLOGIA AGRARIA Boll. Lab. Ent. agr. Filippo Silvestri 61: 25-46 GENNARO VIGGIANI Dipartimento di Entomologia e Zoologia Agraria «Filippo Silvestri», Università degli Studi di Napoli «Federico II», Portici, Italia MERCEDES VELASQUEZ DE RIOS Universidad Nacional Experimental Rómulo Gallegos, Área de Ingenería, San Juan de los Morros, Estado Guárico, Venezuela New Trichogrammatidae (Hymenoptera) from Venezuela Abstract Prouscana lineacalvata n. gen., n. sp., Adryas longiclavata n. sp., A. breviterebrata n. sp., A. intermedia n. sp., Pseuduscana neotropica n. sp., Lathromeroidea longiclavata n. sp., Burksiella altagraciae n. sp. and B. platysetosa n. sp. (Hymenoptera: Trichogrammatidae) are described from Venezuela. Key words: trichogrammatid, Prouscana, Adryas, Pseuduscana, Lathromeroidea, Burksiella, Venezuela. Among the trichogrammatids collected in Venezuela by one of us (M. V.) several undescribed genera and species have been found. Previously VIG- GIANI & VELASQUEZ (2004) described the unusual genus Pteroanomalogramma. The description of a new genus and of seven new species will be given in the present paper. Most terms and acronyms used follow DOUTT & VIGGIANI (1968), others are defined in the text. The holotypes and some paratypes will be preserved in the entomological collection of the Dipartimento di Entomologia e Zoologia Agraria «Filippo Silvestri», Università degli Studi di Napoli «Federico II», Portici (NA), Italia. Other paratypes will be preserved in the entomological collection of the Universidad Nacional Experimental Rómulo Gallegos, Área de Ingenería, San Juan de los Morros, Estado Guárico, Venezuela.

26 Prouscana n. gen. Viggiani et Velasquez Type species: Prouscana lineacalvata n.sp. Viggiani et Velasquez Diagnosis. Antenna without anelli and funicular segments, with four asymmetrical club segments. Mid lobe of mesoscutum and scutellum with fine and longitudinal striation. Fore wing with a peculiar ciliation pattern, characterized by a kind of linea calva. Male genitalia with aedeagus indistinct from the phallobase. Prouscana lineacalvata n. sp. Viggiani et Velasquez Female (Fig. I). Head, antennae (except some brown, mainly on the club), midlobe of mesoscutum, scutellum and legs, yellow. Eyes purple. Pleural parts of mesosoma and metasoma, dark honey. Fore wing infuscate on the blade below the venation. Length: 0.7 mm. Head with posterior ocellar distance slightly longer than twice the ocell-ocular distance. Mandible with 3 teeth (Fig. II, 1); maxillary palp 1-segmented, distally with two setae and an elongate peg sensillum (Fig. II, 2). Antenna (Fig. II, 3) rather short, with the following length/width ratio of scape, pedicel and club: 27/12, 17/15, 55/19. Club subconical, with C1 about twice as wide as long, C2 asymmetrical (length/width: 24/18) as C3 (length/width: 10/15), C4 subconical (length/width: 22/8). Club segments with 1 peg sensillum (PS) and 1 linear sensillum (LS) on C1, 3 PS and 1 LS on C2, 1 PS and 1 LS on C3, 1 PS and 2 LS on C4. Club setae as in Fig. II, 3. Fig. I - Prouscana lineacalvata n. sp. Female.

27 Fig. II - Prouscana lineacalvata n. sp. Female. 1. Mandible. 2. Maxillary palp. 3. Antenna. 4. Part of scutellum, metanotum and propodeum. Mesosoma about as long as metasoma. Pronotum, midlobe of mesoscutum, scutellum, metanotum and propodeum with a thin striate sculpturing (Fig. II, 4). Midlobe of mesoscutum and scutellum each with two pairs of setae. Fore wing (Fig. III, 1) rather broad, about twice as long as wide, widest near apex; relative length of the veins: 20 (submarginal vein): 10 (premarginal vein): 18 (marginal vein): 5 (stigmal vein). Track of basal vein well marked. Neck of the stigmal vein slightly constricted. Submarginal vein with one dorsal seta, premarginal vein with two rather strong setae, marginal vein with three main setae. Blade with one seta before the premarginal vein, RS1 present and followed by a distinct row of setae, which delimitates a kind of linea calva, connected with the first track of the cubital vein. Tracks vein of R, RS2, r- m, C1 and C2 present. Discal ciliation rather dense, covering only about one-third of the blade. Fringe short, longest setae as long as stigmal vein. Hind wing (Fig. III, 2) with three rows of setae; fringe longest setae as long as maximum width of the blade. Legs robust (Fig. IV, 1-3), with rather enlarged fore and hind femura, fore and mid femura with a distal strong seta; middle spur as long as the corresponding basitarsus. Metasoma with tergites mostly with a thin striate sculpturing. Genital sternite as in Fig. IV, 4. Ovipositor not extruded, longer than hind tibia (OL/HTL = 1.71), with very short third valvula (Fig. IV, 5).

28 Fig. III - Prouscana lineacalvata n. sp. Female. 1. Fore wing. 2. Hind wing. Male. Mostly as the female, but antenna with longer setae on the distal club segment (Fig. IV, 6). Genitalia (Fig. IV, 7) simple, tubular, 0.05 mm in length. Etimology In reference to the seta pattern on the fore wing. Material examined. Holotype,, Venezuela, Aragua, Cuyagua, 18.XI.1999, coll. J.L. Garcia. Allotype,, same data as holotype. Paratypes: 1, Venezuela, Aragua, Choroní, 4.VIII.1999, coll. J.L. Garcia; 1, Venezuela, Aragua, Cumboto, 12.VIII.1999, coll. J.L. Garcia. Comment. The new genus Prouscana clearly belongs to the Trichogrammatidae Oligositinae Chaetostrichini (VIGGIANI, 1971) and appears related to the genus Uscana Girault, mainly for the antennal features. For some characters concerning the dense discal ciliation, the new genus appears allied to Tumidifemur Girault. PINTO (2006) apparently widened the definition of the latter genus including also specimens here used to described Prouscana.

29 Fig. IV - Prouscana lineacalvata n. sp. Female. 1. Fore leg. 2. Mid leg. 3. Hind leg. 4. Genital sternite. 5. Part of the ovipositor. Male. 6. Distal segments of the club. 7. Copulatory organ. Adryas Pinto et Owen Adryas longiclavata n. sp. Viggiani et Velasquez Diagnosis. The new species can be distinguished by the combination of the following characters: brown antennae with long club, sculpturing consisting of cells mostly longer than wide with transversal corrugations within, subequal scutellar setae, ovipositor twice as long as hind tibia. Female. Brown, fronto-vertex, tibiae and tarsi, light. Fore wing fumate

30 on the basal half. Length: 0.65 mm. Mandible tridentate, with the two external teeth stronger. Maxillary palp one-segmented, subcylindrical, 3 times as long as wide, at apex with two setae, one very short and the other onethird shorter than palp length, and an elongate peg sensillum. Antenna (Fig.V, 1) with the following length/width ratio of scape, pedicel and club: 12/4, 8/6, 35/7. Club subconical, with C1-C4 asymmetrical, C1 very small and combined with C2 (length/width: 3/7) (Fig. V, 2), C3 (length/width: 13/7) sligthly larger than C4 (length/width: 12/5), C5 conical (length/width: 14/3), slightly longer than the C4, but much narrower. Club segments with Fig. V - Adryas longiclavata n. sp. Female. 1. Antenna. 2. First and second club segment. 3. Particular of midlobe of mesoscutum. 4. Particular of scutellum.

31 Fig. VI - Adryas longiclavata n. sp. Female. 1. Fore wing. Male. 2. Antenna. 3. Copulatory organ. 1 PS on C1, 2 PS and 1 LS on C2, 2 PS and 1 LS on C3, 1 PS and 2 LS on C4, 1 PS and 4 LS on C5. Midlobe of mesoscutum with 2 pairs of setae and sculpturing consisting of cells mostly longer than wide with transversal corrugations within (Fig. V, 3); scutellum with 2 pairs of subequal setae and sculpturing as in Fig V, 4. Propodeum disc broadly subtriangular and slightly longer than metanotum, with 2 small setae near each spiracle. Fore wing (Fig. VI, 1 ) twice as long as wide; vein length ratios: 20 (SV): 8 (PV): 13 (MV): 7 (STV). Marginal vein with 3 main setae. RS1 present, discal ciliation arranged in distinct rows. Fringe longest setae about one/sixth of the discal maximum width. Hind wing with 1 median row of setae. Legs normal with middle spur as long as the corresponding basitarsus. Metasoma longer than mesosoma (25/20); ovipositor slightly extruded, twice as long as the hind tibia. Male. As female, but antenna shorter (Fig. VI, 2). Copulatory organ simple (Fig. VI, 3), tubular, with two ventral setae, 0.08 mm in length.

32 Material examined. Holotype,, Venezuela, S.J. Morros URG, 8.XII.2002, coll. M. Velasquez and Z. Tiape. Allotype, 1, Venezuela, Aragua, Cumboto, 8.XII.1998, coll. J.L. Garcia. Paratypes: 1, Venezuela, S.J. Morros URG, 5.XII.2002, coll. M. Velasquez and Z. Tiape; 2, Venezuela, Guarico, 5.IX.2002, coll. M. Velasquez and Z. Tiape; 1, Venezuela, S.J. Morros URG, 4.XII.2002, coll. M. Velasquez and Z. Tiape; 1, Venezuela, Aragua, Camatagua, 20-27.VIII.2003, coll. M. Velasquez and Z. Tiape; 1 and 1, Venezuela, Aragua, Camatagua, 15-18.X.2003, coll. M. Velasquez and Z. Tiape. Etimology In reference to the long antennal club. Comment The new species is to be included among the species of Adryas described by PINTO & OWEN (2004) having a symmetrical last club segment and midlobe of mesoscutum with two pairs of setae. Adryas breviterebrata n. sp. Viggiani et Velasquez Diagnosis. The new species can be distinguished by the combination of the following characters: symmetrical antenna with spatulate sensilla and long setae, 2 pairs of setae on midlobe of mesoscutum and sculpturing consisted of cells mostly longer than wide with transversal corrugations within, ovipositor very short, shape of the simple copulatory organ. Female. Brown, fronto-vertex, tibiae and tarsi, light. Fore wing fumate on the basal half. Length: 0.65 mm. Mandible tridentate, with the two external teeth stronger. Maxillary palp one-segmented, subcylindrical, 3 times as long as wide, at apex with two setae, one very short and the other onethird shorter than palp length, and an elongate peg sensillum. Antenna similar to that of the male (Fig. VIII, 1), with following length/width ratio of scape, pedicel and club: 12/5, 8/6, 35/11. Club subconical, with C1-C4 asymmetrical, C1 very small and combined with C2 (length/width: 3/7), C3 (length/width: 13/7) sligthly larger than C4 (length/width: 12/5), C5 conical (length/width: 14/3), slightly longer than C4, but much narrower. Club segments with 1 PS on C1, 2 PS and 1 LS on C2, 2 PS and 1 LS on C3, 1 PS and 2 LS on C4, 1 PS and 4 LS on C5. Shape of PS tipically spatulate (Fig. VIII, 2). Club segments C3-C5 with long setae as in Fig. VIII,1. Midlobe of mesoscutum with 2 pairs of setae and sculpturing consisted of cells mostly longer than wide with transversal corrugations within (Fig. VII, 1); scutellum with 2 pairs of subequal setae. Propodeum disc broadly subtriangular and slightly longer than metanotum, with 2 small setae near each spiracle. Fore wing (Fig. VII, 2) twice as long as wide; vein length ratios: 20 (SV): 8 (PV): 13 (MV): 7 (STV). Marginal vein with 3 main setae. RS1 present, discal ciliation arranged in distinct rows. Fringe longest setae about one/sixth of the discal maximum width. Hind wing with 1 median row of setae. Legs normal with middle spur as long as the corresponding ba-

33 Fig. VII - Adryas breviterebrata n. sp. Female. 1. Particular of midlobe of mesoscutum. 2. Fore wing. 3. Distal part of metasoma. sitarsus. Metasoma longer than mesosoma (25/20); ovipositor (Fig. VII, 3) slightly extruded, twice as long as the hind tibia. Male. As female, but antenna shorter (Fig. VIII, 1). Copulatory organ simple (Fig. VIII, 3), with a subtriangular profile, 2 small ventral setae, 0.08 mm in length. Material examined. Holotype:, Venezuela, Guárico, S.J. Morros URG, 7.VII.2002, coll. M. Velasquez and Z. Tiape. Allotype: 1, same data of holotype. Paratypes: 1 and 3, same data of holotype. Etimology In reference to the very short ovipositor.

34 Fig. VIII - Adryas breviterebrata n. sp. Male. 1. Antenna. 2. Spatulate sensillum. 3. Copulatory organ. Adryas intermedia n. sp. Viggiani et Velasquez Diagnosis. The species can be distinguished by the following combination of characters: symmetrical club with a narrow last segment, 2 pairs of setae and sculpturing on midlobe of mesoscutum, RS1 track and short fringe on fore wing, ovipositor/hind tibia ratio. Female. Brown, with fronto-vertex, sides of mid lobe of mesoscutum, partially metanotum and propodeum, legs (except coxa and hind femur) light. Fore wing fumate, particularly below the marginal vein. Length: 0.7 mm. Mandible tridentate. Maxillary palp one-segmented, subcylindrical, 3 times as long as wide, with a terminal long seta, slightly longer than palp, an elongate sensorial peg and a small seta. Antenna (Fig. IX, 1) with scape, pedicel, anellus, and a five segmented club. Measurement (length/width) of antennal scape, pedicel, and club: 26/11, 16/12, 55/17. Club subconical, with C1 very short (length/width: 5/14), C2 transverse (length/width: 10/15), C3 asymmetrical (length/width: 6/15), C4 symmetrical (length/width: 15/15), C5 subcylindrical, narrow (length/width: 22/5). Club segments with 3 PS on C1, 4 PS and 1 LS on C2, 4 PS and 1 LS on C3, 1 PS and 2 LS on C4, 1 PS and 3 LS on C5. Midlobe of mesoscutum with 2 pairs of setae (Fig.IX, 2) and sculpturing, from the anterior margin to the level of the first pair of setae, represented by cells about twice as long as wide, with one or two corrugations within; in the remaining part the cells become very narrow, giving an apparent striation. Propodeal disc short, as long as the corresponding part of the metanotum, with 3 small setae near each spiracle. Fore wing (Fig. IX, 3) twice as long as wide; vein length ratios: 24 (SV): 10 (PV): 12 (MV): 6 (STV). SV with 1 dorsal seta, PV with 2 setae, MV with 3 main setae. Distal half of the costal cell with 4 setae. PV preceded by a row of 5 setae and by a patch of

35 Fig. IX - Adryas intermedia n. sp. Female. 1. Antenna. 2. Particular of midlobe of mesoscutum and scutellum. 3. Fore wing. about 15 very small peg sensilla, RS1 present. Discal ciliation mostly arranged in distinct lines. Fringe rather short, with longest setae about onetenth of the discal width. Hind wing with two submarginal and one median rows of setae. Legs normal, with mid tibia spur as long as corresponding basitarsus. Ovipositor not extruded, slightly longer than hind tibia (35/30). Male. Unknown. Material examined. Holotype,, Venezuela, Guarico, FCA, Guárico, Finca Mata Negra, Las Lajas, 1.IX.2002, coll. M. Velasquez and Z. Tiape. Paratype: 1, Venezuela, Aragua, Camatagua 29.X.2003, coll. M. Velasquez and Z. Tiape. Etimology. In reference to the intermediate length of ovipositor in comparison with A. longiclavata and A. breviterebrata.

36 Lathromeroidea Girault Lathromeroidea longiclavata n. sp. Viggiani et Velasquez Diagnosis. The long antennal club, combined with the fore wing and metasoma characters, distinguish the new species from the congeneric. Female. Black. Eyes red. Length: 0.75 mm. Mandible with two strong teeth. Maxillary palp subcylindrical, three times as long as wide, with a distal long seta, as long as the palp, a short seta and an elongate peg sensillum. Head with strong setae on the fronto-vertex and rather long also on Fig. X - Lathromeroidea longiclavata n. sp. Female. 1. Antenna. 2. Particular of midlobe of mesoscutum and scutellum. 3. Fore wing.

37 the eyes. Antenna (Fig. X,1) with scape, pedicel, anellus, and a five segmented club. Measurement (length/width) of antennal scape, pedicel, and club: 30/10, 20/10, 50/16. Club subconical, rather asymmetrical, with C1 very short (length/width: 3/12), C2 transverse (length/width: 6/18) as C3 (length/width: 6/17), C4 longer (length/width: 18/15) than C3, C5 subcylindrical, narrow (length/width: 22/8). Club segments with 2 PS on C1, 2 PS on C2, 1 PS and 1 LS on C3, 1 PS and 2 LS on C4, 1 PS and 2 LS on C5. Midlobe of mesoscutum with 2 pairs of strong setae as scutellum; sculpturing of both constituted of cells without internal corrugations (Fig. X, 2). Propodeal disc short, about twice as long as the corresponding part of metanotum, with 3 setae near each spiracle. Fore wing (Fig. X, 3) 2.2. times as long as discal width; vein length ratios: 23 (SV): 10 (PV): 17 (MV): 6 (STV). SV with 1 dorsal seta, PV with 2 setae, MV with 3 main setae and a row of 4 setae. SV subtriangular. Distal half of the costal cell with a row of 5 marginal setae. PV preceded by a row of 5 setae, RS1 present. Discal ciliation mostly arranged in distinct lines. Fringe rather short, with longest setae about one-third of the discal width. Hind wing with a median complete row of setae and two incomplete submarginal rows. Legs normal, with mid tibia spur simple, like a strong seta, longer than corresponding basitarsus (15:10). Metasoma with tergites I-IV, only laterally with a simple sculpturing consisted of a transverse corrugation (Fig. XI, 1). Ovipositor slightly extruded, 1.7 times as long as hind tibia (60/35). Male. Similar to the female, but antenna (Fig. XI, 2) with a narrower club and longer setae on the distal club segment. Copulatory organ (Fig. XI, 3) simple, tubular, 0.09 mm in length. Material examined. Holotype:, Venezuela, Guárico, Finca Mata Negra, Las Lajas, 4.IX.2002, coll. M. Velasquez and Z. Tiape. Allotype: 1, Venezuela, Guarico, S.J. Morros URG, 4-7.VII.2002, coll. M. Velasquez and Z. Tiape. Etimology. In reference to the long antennal club. Comment. Recently the genus Lathromeroidea was redefined by PINTO (2006). The new species should be included in the group A of the mentioned author. Pseuduscana Pinto Pseuduscana neotropica n. sp. Viggiani et Velasquez Diagnosis. Mid lobe of mesoscutum with two pairs of setae; longest fringe setae of the fore wing one/third of the disc. Copulatory organ

38 Fig. XI - Lathromeroidea longiclavata n. sp. Female. 1. Side view of metasoma. Male. 2. Antenna. 3. Copulatory organ. with aedeagus fused with the phallobase which lacks of parameres and digiti. Male. Brown, with mesosoma, legs, except hind femur, and antennae, lighter. Fore wing infuscate, mostly below the venation. Eyes red. Length: 0.5-0.6 mm. Mandible tridentate with two external strongest teeth. Maxillary palp 1-segmented distally with two setae and an elongate peg sensillum. Antenna (Fig. XII, 1) with the following ratio length/width of scape, pedicel and club: 25/10, 14/12, 38/13. Club rather narrow with C1 very short (length/width: 4/10), C2 slightly longer than wide (length/width: 13/12), C3

39 Fig. XII - Pseuduscana neotropica n. sp. Male. 1. Antenna. 2. Copulatory organ. shorter than C2 (length/width: 10/8), C4 conical, longer than C2 (length/width: 15/8). Club segments with 2 PS on C1 and C2, 1 PS and 1 LS on C3 and C4, and 1 PS and 2 LS on C5. Midlobe of mesoscutum with 2 pairs of setae and sculpturing consisting of cells mostly longer than wide with longitudinal corrugations within (Fig. XIII, 2); scutellum with 2 pairs of setae, but with the anterior pair about half length of posterior one and sculpturing similar that on midlobe. Propodeum medially as long as metanotum. Fore wing (Fig. XIII, 3) about twice as long as wide; vein length ratios: 30 (SV): 15 (PV): 23 (MV). RS1 present, discal ciliation arranged in rather distinct rows. Fringe longest setae about one/fourth of the discal maximum width. Hind wing with 1 median row of setae. Legs normal with middle tarsus slightly shorter than corresponding tibia; middle spur shorter than basitarsus. Copulatory organ (Fig. XII, 2) 0.1 mm in length, with phallobase fused with the aedeagus and distally provided, on each side, of a triangular projection; parameres and digiti not developed. Female. As male, but antenna (Fig. XIII, 1) with two whorls of setae on C3. Ovipositor shorter than hind tibia (18/23).

40 Fig. XIII - Pseuduscana neotropica n. sp. Female. 1. Antenna. 2. Particular of midlobe of mesoscutum and scutellum. 3. Fore wing. Material examined. Holotype:, Venezuela, Aragua, Cuyagua, 11.XI. 1999, coll. J.L. Garcia. Allotype: 1, Venezuela, Aragua, Cumboto, 12.VIII.1999, coll. J. L. Garcia. Etimology. In reference to the site from which the species was collected. Comment. P. neotropica can be distinguished from the only described species of the genus Pseuduscana Pinto (PINTO, 2006), P. sola Pinto, by several characters: midlobe of mesoscutum with two pairs of setae, longest fringe setae of the fore wing one/third of the disc and copulatory organ with aedeagus fused with the phallobase which lacks of parameres and digiti. According to PINTO (2006) the genus includes species very homogeneous in female sex, yet the male genitalia are more variable and can help in the specie diagnosis.

41 Burksiella De Santis PINTO (2006) renewed the status of Burksiella De Santis, which was treated as a junior synonym of Zagella by DOUTT & VIGGIANI (1968). Burksiella altagraciae n. sp. Velásquez et Viggiani Diagnosis. The new species can be distinguished by the subglobular second funicular segment wider than basal club segment, with 7-8 placoid sensilla, rather large hind wing with four lines of setae, and the male genitalia features, with rather short phallobase provided with parameres and digiti. Female. Body robust, brown with some lighter parts: antennal scape, apex of tibia and femora, tarsomeres, stripes on metasoma. Length: 0,94 (0,90-0,98) mm. Head wider than thorax; eyes red. Mandible with three teeth. Maxillary palp 1-segmented (Fig. XIV, 1). Antenna (Fig. XIV, 2) with Fig. XIV - Burksiella altagraciae n. sp. Female. 1. Maxillary palp. 2. Antenna. 3. Funicular segments. 4. Particular of midlobe of mesoscutum. 5. Fore wing. Male. 6. Copulatory organ, side view. 7. The same, front view.

42 scape 2.0-2.5 as long as wide; 2 very short anelli; funicle 2-segmented (Fig. XIV, 3) with the first discoidal and asymmetric provided with 5-6 PS and a trichoid sensillum, the second segment much developed, subglobular, wider than club segments, with 7-8 LS and several PS. Club subconic, 3-segmented and rather asymmetric: C1 with 3 LS and 7-8 PS; C2 with 2-3 LS and 1 PS; C3 progressively pointed to the apex, with 1-2 LS and 1 PS. Midlobe of mesoscutum and scutellum with sculpture as in Fig. XIV, 4 and each with two pairs of setae. Fore wing (Fig. XIV, 5) 0.60 as wide as long, apically truncate, with very short marginal cilia. Venation extended to the half of the wing length: costal cell with 7-8 setae on the external margin and 1-2 setae on the antero-apical area; parastigma sligthly wider at base with a pair of strong setae; external margin of the marginal vein with 4 strong and 6 smaller setae, internal margin with 4-5 setae; stigmal vein with a basal seta, 2 setae on the apex and a very short seta at the base of uncus. Relative length of the veins: submarginal 12.5, parastigma 5.5, marginal 7.5, stigmal 5. SR1 prominent and markedly curved. Ciliation of the disc arranged in 19-20 lines. Hind wing rather large with 4 lines of setae, some (first and fourth) incomplete at base. Legs robust; middle spur as long as basitarsus. Ovipositor extended in the total length of the gaster, sligthly extruded, 2.3-2.5 as long as hind tibia. Hypopygium triangular, longer than wide, 0.47 the length of the ovipositor. Male. Similar to the female, but the antennal club is smaller and lighter. Male genitalia (Fig. XIV, 6-7) with phallobase rather short, about as long as wide, distally with parameres and digiti; aedeagus rather short, with distinct apodemes about as long as its body. Length: 0.06 mm. Etimology. In reference to the name of a people in a north-eastern area of the Guárico state, Venezuela. Material examinated. Holotype:, Venezuela, Guárico, Altagracia de Orituco, Finca El Renacer, 7-24.VII.2004, in Malaise trap, coll. M. Velasquez and Z. Tiape. Allotype:, same data of holotype. Paratypes: 5 and 2, same data of holotype, except one specimen collected on 3.X.2005. The type material will be deposited in the Colección Regional de Insectos de la Universidad Rómulo Gallegos. Burksiella platysetosa n. sp. Viggiani et Velasquez Diagnosis. The species is easily recognized by the cluster of flatted setae on the marginal vein of fore wing. Female. Body brown, with lighter areas on mesoscutum. Eyes red.

43 Legs with distal part of tibiae and tarsi light. Fore wings with infuscation beneath the venation area. Length: 0.7 mm. Mandibles 3-toothed. Maxillary palp 1-segmented. Antenna (Fig. XV, 1) with the following length/width ratio of scape, pedicel, funiculus and club: 25/10, 16/13, 18/20, 40/18. F1 very short and attached to F2, the latter globular rather inflated (length/width: 18/20). Club with C1 and C2 asymmetrical and subequal, C3 conical, longer than C2 (length/width: 20/8). Funicular segments with 1 PS on F1 and 3 PS and 2 LS on F2. Club segments with 5 PS and 1 LS on C1, 1 PS and 1 LS on C2, 1 PS and 3 LS on C3. Setae as in Fig. XV, 1. Midlobe of mesoscutum and scutellum both with a pair of setae and sculpturing consisted of subpolygonal cells with longitudinal corrugation within (Fig. XV, 2-3). Propodeum medially as long as metanotum. Fore Fig. XV - Burksiella platysetosa n. sp. Female. 1. Antenna. 2. Particular of midlobe of mesoscutum. 3. Particular of scutellum.

44 wing (Fig. XVI, 1) about 1.7 times as long as wide; vein length ratios: 20 (SV): 12(PV): 12 (MV), 5 (STV). SV with one dorsal seta, PV with 2 setae, MV rather enlarged, with 3 main setae and a cluster of 12-14 flat setae (Fig. XVI, 2), STV ovoidal with a short neck. Costal cell with 4-5 marginal seta on the distal half. A patch of 8-10 very small peg sensilla preceding the premarginal uncus. RS1 present and curved, discal ciliation arranged in about 20 distinct rows. Fringe longest setae about one/tenth of the discal maximum width. Hind wing with 2 submarginal and 1 median row of setae. Legs normal with mid spur slightly longer than basitarsus (8/6). Metasoma longer than mesosoma (65/60). Ovipositor not extruded, longer than hind tibia (38/26). Fig. XVI - Burksiella platysetosa n. sp. Female. 1. Fore wing. 2. Particular of fore wing.

45 Fig. XVII - Burksiella platysetosa n. sp. Male. 1. Antenna. 2. Copulatory organ. Male. As the female. Antenna (Fig. XVII, 1) with rather shorter club, but with longer setae on the last segment. Copulatory organ (Fig. XVII, 2) with tubular phallobase, fused with aedeagus and without parameres and digiti. Length: 0.14 mm. Etimology. In reference to the peculiar features of the setae on the marginal vein. Material examined. Holotype:, Venezuela, Aragua, Cuyagua, 20.V.1999, coll. J.L. Garcia. Allotype:, Venezuela, Aragua, Cuyagua, 2.II.1999, coll. J.L. Garcia. Paratypes: 1,Venezuela, Aragua, Cuyagua, 18.XI.1999, coll. J.L. Garcia; 1, Venezuela, Aragua, Cumboto, 2.XII.1999, coll. J.L. Garcia. REFERENCES DOUTT, R. & VIGGIANI, G. 1968 The classification of the Trichogrammatidae (Hymenoptera: Chalcidoidea). Proc. Cal. Ac. Sc. (fourth ser.) 35 (20): 477-586. PINTO, J. 2006 A review of the new world genera of Trichogrammatidae (Hymenoptera). J. Hym. Res. 15 (1): 38-163. PINTO, J. & OWEN, A. 2004 Adryas, a new genus of Trichogrammatidae (Hymenoptera: Chalcidoidea) from the new world tropics. Proc. Entomol. Soc. Wash. 106 (4): 905-922. VIGGIANI, G. 1971 Ricerche sugli Hymenoptera Chalcidoidea XXVIII. Studio morfologico comparativo dell armatura genitale esterna maschile dei Trichogrammatidae. Boll. Lab. Ent. agr. Filippo Silvestri 29: 181-122. VIGGIANI, G. & VELASQUEZ, M. 2004 Description of Pteroanomalogramma singulare n.

46 gen., n. sp. (Hymenoptera: Trichogrammatidae) from Venezuela. Boll. Lab. Ent. agr. Filippo Silvestri 59: 87-91. Author for correspondence: Gennaro Viggiani, Dipartimento di Entomologia e Zoologia Agraria «Filippo Silvestri», Università degli Studi di Napoli «Federico II», Via Università 100 80055 Portici (NA), Italia. E-mail: genviggi@unina.it Printed on December 28 th 2006.