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EMA/COMP/472601/2009 Rev.1 Committee for Orphan Medicinal Products Expanded human allogeneic mesenchymal adult stem cells extracted from adipose tissue for the treatment of anal fistula First publication 14 October 2009 Rev.1: sponsor s name change 7 June 2013 Disclaimer Please note that revisions to the Public Summary of Opinion are purely administrative updates. Therefore, the scientific content of the document reflects the outcome of the Committee for Orphan Medicinal Products (COMP) at the time of designation and is not updated after first publication. On 8 October 2009, orphan designation (EU/3/09/667) was granted by the European Commission to Cellerix S.A., Spain, for expanded human allogeneic mesenchymal adult stem cells extracted from adipose tissue for the treatment of anal fistula. In February 2013, Cellerix S.A. changed name to TiGenix S.A.U. What is anal fistula? An anal fistula is an abnormal passageway that develops between the rectum (the lower part of the large intestine that stores faeces) and the outside of the body. This results in abnormal discharge of faeces through an opening other than the anus. Anal fistulae are usually caused by an infection or an abscess (collection of pus) in the anus. They can also result from other diseases that cause long-term inflammation of the bowel. Patients with an anal fistula have constant pain, sometimes accompanied by swelling and irritation of skin around the anus, leakage of pus, diarrhoea and fever. Anal fistula is a long-term debilitating disease because it can lead to incontinence (a lack of control over the opening of the bowels) and sepsis (blood infection). 7 Westferry Circus Canary Wharf London E14 4HB United Kingdom Telephone +44 (0)20 7418 8400 Facsimile +44 (0)20 7418 8416 E-mail info@ema.europa.eu Website www.ema.europa.eu An agency of the European Union European Medicines Agency, 2013. Reproduction is authorised provided the source is acknowledged.

What is the estimated number of patients affected by the condition? At the time of designation, anal fistula affected approximately 2.3 in 10,000 people in the European Union (EU). This was equivalent to a total of around 116,000 people *, and is below the ceiling for orphan designation, which is 5 people in 10,000. This is based on the information provided by the sponsor and the knowledge of the Committee for Orphan Medicinal Products (COMP). What treatments are available? At the time of designation, no satisfactory method were authorised in the EU for the treatment of anal fistula. Some anal fistulae heal without any specific treatment, but surgery is usually necessary. This involves stretching the gut tissue or cutting the sphincter muscles (the muscles that control the opening and closing of the anus), although this may cause problems such as incontinence. How is this medicine expected to work? This medicine is made up of mesenchymal stem cells that are extracted from the adipose (fat) tissue of a donor. To make this medicine, the cells are isolated and cultivated using a technique called ex vivo expansion to increase their number. When these cells are injected into the walls of the fistula, they are expected to send signals that reduce the activity of the immune system and inflammation. Once the inflammation in the fistula has subsided, new tissue can start to grow, helping the fistula to heal. What is the stage of development of this medicine? The effects of expanded human allogeneic mesenchymal adult stem cells extracted from adipose tissue have been evaluated in experimental models. At the time of submission of the application for orphan designation, clinical trials in patients with anal fistula were ongoing. At the time of submission, this medicine was not authorised anywhere in the EU for anal fistula or designated as an orphan medicinal product elsewhere for this condition. In accordance with Regulation (EC) No 141/2000 of 16 December 1999, the COMP adopted a positive opinion on 8 July 2009 recommending the granting of this designation. * Disclaimer: For the purpose of the designation, the number of patients affected by the condition is estimated and assessed on the basis of data from the European Union (EU 27), Norway, Iceland and Liechtenstein. At the time of designation, this represented a population of 504,800,000 (Eurostat 2009). EMA/COMP/472601/2009 Page 2/5

Opinions on orphan medicinal product designations are based on the following three criteria: the seriousness of the condition; the existence of alternative methods of diagnosis, prevention or treatment; either the rarity of the condition (affecting not more than 5 in 10,000 people in the EU) or insufficient returns on investment. Designated orphan medicinal products are products that are still under investigation and are considered for orphan designation on the basis of potential activity. An orphan designation is not a marketing authorisation. As a consequence, demonstration of quality, safety and efficacy is necessary before a product can be granted a marketing authorisation. For more information Sponsor s contact details: TiGenix S.A.U. Marconi 1, Parque Tecnológico de Madrid Tres Cantos 28760 Madrid Spain Telephone: +34 91 804 92 64 Telefax: +34 91 804 92 63 E-mail: maria.pascual@tigenix.com For contact details of patients organisations whose activities are targeted at rare diseases see: Orphanet, a database containing information on rare diseases which includes a directory of patients organisations registered in Europe. European Organisation for Rare Diseases (EURORDIS), a non-governmental alliance of patient organisations and individuals active in the field of rare diseases. EMA/COMP/472601/2009 Page 3/5

Translations of the active ingredient and indication in all official EU languages 1, Norwegian and Icelandic Language Active ingredient Indication English Bulgarian Czech Danish Dutch Estonian Finnish French German Greek Hungarian Italian Latvian Lithuanian Maltese Polish Portuguese Expanded human allogeneic mesenchymal adult stem cells extracted from adipose tissue Човешки алогенни мезенхимни стволови клетки, извлечени от мастна тъкан на възрастни индивиди Lidské allogenní mezenchymální dospělé buňky, odebrané z adipózní tkáně Humane allogene mesenkymale adulte stamceller, ekstraheret fra fedtvæv Geëxpandeerde humaan allogene mesenchymale volwassen stamcellen, geëxtraheerd uit adipeus weefsel Rasvkoest saadud inimese allogeensed mesenhümaalsed täisealised tüvirakud Ihmisen allogeenisia mesenkymaalisia somaattisia kantasoluja, jotka on otettu rasvakudoksesta Cellules souches humaines mésenchymateuses allogéniques adultes extraites de tissu adipeux Humane aus Fettgewebe extrahierte allogene mesenchymale adulte Stammzellen Ανθρώπινα αλλογενή μεσεγχυματικά ενήλικα βλαστικά κύτταρα προερχόμενα από λιπώδη ιστό Zsírszövetből kivont allogén mesenchimalis felnőtt emberi őssejtek Cellule staminali mesenchimali adulte umane allogeniche, estratte da tessuto adiposo No taukaudiem iegūtas ekspensētas pieauguša cilvēka allogēnas mezenhimālās cilmes šūnas Pagausintos žmogaus alogeninės mezenchimos brandžios kamieninės ląstelės, gautos iš riebalinio audinio Ċelluli staminali adulti alloġeneiċi mesenkimali umani estratti mit-tessut adipożu Ludzkie alogeniczne mezenchymalne dorosłe komórki macierzyste uzyskane z tkanki tłuszczowej Células estaminais alogénicas adultas de mesênquima humano extraídas do tecido adiposo Treatment of anal fistula Лечение на анална фистула Léčba anální píštěle Behandling af analfistler Behandeling van anusfistel Anaalfistuli ravi Anaalifistelin hoito Traitement des fistules anales Behandlung von Analfisteln Θεραπεία πρωκτικού συριγγίου Végbélsipoly kezelése Trattamento della fistola anale Anālas fistulas ārstēšana Analinės fistulės gydymas Kura ta fistla anali Leczenie przetok odbytu Tratamento de fístulas anais 1 At the time of designation EMA/COMP/472601/2009 Page 4/5

Language Active ingredient Indication Romanian Slovak Slovenian Spanish Swedish Norwegian Icelandic Celule stem umane adulte mezenchimale alogene multiplicate (expandate ex vivo) extrase din ţesut adipos Expandované ľudské alogénne mezenchýmové adultné kmeňové bunky získané z tukového tkaniva Človeške alogenske mezenhimske odrasle zarodne celice, vzete iz maščobnega tkiva Células madre expandidas alogénicas humanas adultas de origen mesenquimal extraídas de tejido adiposo Humana allogena mesenkymala vuxna stamceller extraherade ur fettvävnad Humane allogene mesenkymale voksne stamceller ekstrahert fra adipøst vev Fullorðnar stofnfrumur úr bandvefskími úr ósamgena einstaklingi dregnar úr fituvef. Tratamentul fistulei anale Liečba análnej píšťaly Zdravljenje analne fistule Tratamiento de la fístula anal Treatment of Anal FistulaBehandling av analfistel Behandling av analfistel Meðferð við endaþarmsfistli EMA/COMP/472601/2009 Page 5/5