MP-RS232 CPM Photoncounting Module with integrated RS232 PC Interface

MP-RS232 CPM Photoncounting Module with integrated RS232 PC Interface Operating Instructions DETECTION SOLUTIONS Introduction The Photon Counting Unit MPXXX- RS232 series is designed for applications in all fields of single photon detection, e.g. chemo-luminescence, bioluminescence, fluorescence, in vitro assay, environmental measurements or pure research. It is an easy to use module, containing the Channel Photomultiplier, high voltage power supply, discrimination amplifier, pulse shaper for fast output pulses and a microcontroller unit for data transmission to the RS232 interface of a Personal Computer. All operating functions can be controlled by software commands. Free download demo software is available. Features and Benefits Extremely low noise and highest detection sensitivity Cost saving by read out via RS232 Plug and Play Uniquely gateable via electronic shutter High dynamic range Broad spectral sensitivity Applications Fluoroscopy Luminescence Hygiene Monitoring Particle Sizing www.excelitas.com

Table of Contents Functional Specification. 3 Operating Conditions CPM Specification Digital Functions Measuring Process Hardware / Software Requirements. 3 Definitions. 4 Gate Kill High Voltage Counting Time Pause Time Number of Count Intervals Set Up Conditions of Terminal Program Software Description.5.7 Starting Measurement Command Overview Adjustment Commands Output Commands Counting Commands In Case of Overcurrent Detection.13 Power Supply Connections Dimensions.13.13 www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 2

1. Functional Specification 1.1 Operating Conditions Parameter Remark Min Typ Max Unit Supply voltage 5 5 5.5 volts dc Input current @ max. count rate 300 ma Settling time Warm-Up time Over-illumination protection: (time to stabilize HV after supply voltage applied) 1 sec with High Voltage switched on) 5 minutes active quenching control (internal) 5.5 Linear count rate: 5 Active Gate Control GATE voltage V gate Maximum ratings via software command active high Mega Counts/sec Mega Counts/sec h to l set time Vca to Vch-ent +100 V 150 nano sec l to h set time Vca to Vch-ent - 100 V 150 nano sec Input voltage +5.5 Volts dc Operating temperature 5 40 C Storage temperature -20 50 C Weight Digital Functions depending on module format 350 450 g Interface RS232 57,600 bit/sec Read out Intervalls 10 60000 msec Mega Output Count Rate 10 Counts/sec Dead Time between two reading cycles 10 µsec Table 1: Functional Specification 1.2 CPM Specification The MPRS232 modules can be equipped with all standard PKI CPM types. For individual data, please refer to the CPM Product catalogue or contact PerkinElmer Optoelectronics 1.3 Digital Functions: Serial Interface: RS-232, Transmission rate 57.600 bit /s Intervals of measurements: 10ms to 1min Installation of Read Out pause between two read-out cycles possible Max. Counting rate: 10 Mega counts/sec (Mcps, default setting: 5.5 Mcps) Setting of Gating-function via terminal program www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 3

Measuring of CPM high voltage via a HV-monitor possible Online display of various measuring values: o Gating o High Voltage o Temperature 1.4 Measuring process: Overflow detection in counting mode (max. approx 16 Mio. within one Counting interval) Max. count rate: approx. 10Mega counts per second Dead time between two measuring cycles: approx. 10µsec (Recommendation: measuring time 1 sec ) 2. Hardware /Software Requirements: Personal Computer with Windows 9X/2000/XP/VISTA/7 and serial interface (RS232) Terminal Program like HyperTerminal, TeraTerm Connecting Cable Serial, 9-pol D-SUB male/female, (V24 Extension Cable 1:1) 5.0 VDC Power Supply, 300mA 3. Definitions: 3.1 Gate: In case of Gating, the following process happens: A TTL high signal (internally or externally generated) at the Gate input of the High voltage power supply (hvps) triggers an hvps internal circuit which immediately switches cathode potential more positive than Channel Entrance Voltage so the cathode cannot emit photoelectrons into the channel vacuum. The Gate function fulfils two purposes: a) Over illumination protection When accidentally too high light intensities enter the window of the ChannelPhotomultiplier, which would cause count rates, exceeding the maximum specified count rate, the Gate TTL pulse is generated internally (by the over illumination detection circuit) b) Electronic shutter If operator intends to use the gate function as an electronic shutter (e.g. to switch the cathode blind during illuminating the probe by excitation light) the TTL pulse is sent externally to the Gate input or generated via software command. It can either be enabled (command: SG or disabled, command SG0 ) or by striking keyboard key g which toogles between enabled and disabled 3.2 Kill: In case of Kill function is activated, the power supply switches to stand-by mode with main output voltage turning to 0 volts thus all related potentials turning to 0, too. This mode may be useful for repair-, maintenance- or safety purposes. It can either be enabled (command: SK1 or disabled, command SK0 ) or by striking keyboard key k which toogles between enabled and disabled 3.3 HighVoltage High Voltage is the internal high voltage power supply. The voltage output displayed on the screen is in Volts. It can either be enabled (command: SV1 or disabled, command: SV0 ) www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 4

3.4 Counting time Counting time (msec) defines the period of time, the microcontroller accumulates the incoming photons. Default value is 1000msec. It can be changed by command (Set Time) STxxx, where xxx is between 10ms min. and 60000ms max.. 3.5 Pause Time Pause time (msec) defines the period of time between two measuring cycles. Default value is 0ms. During Pause time, no signals (counts) are read out. 3.6 Number of count intervals Number of count intervals defines the number of read outs to be taken by the microcontroller. Default value is 0 (infinite). So duration of one measurement cycle is determined by counting time, Pause time and number of count intervals. 4. Set Up Conditions of Terminal Program (e.g. Hyperterminal TM, TeraTerm) 1. Select your communication Port Please check if communication port either is COM1, COM2 or any other existing COM port. The matching CPM port has to be configured as: Baudrate 57600 (Bits per sec) Databits 8 Parity : No Stopbits 1 Steam Control: Hardware If you run Hyperterminal for the first time, you may be asked to enter a name for the new connection. Please select a distinct name like e.g. CPMRSModule. Any time you re-run this program, just double click the icon, you saved the configuration under. www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 5

Fig. 1: Start Screen when first time using HyperTerminal Once the above step has been completed, you can enter, change or modify the Set-Up parameters according the above described requirements. Fig. 2: Menu for Serial Port Selection www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 6

Fig.3: Set-Up Menu for Serial Port configuration Fig.4: Configuration of appropriate Line Feed function www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 7

5. Software Description for MPRs Photoncounting Modules 5.1 Starting Measurements If you are using the provided input power adapter cable, leads are determined as follows: Red: Black: Orange (optional): Black (optional): +5 Vdc 0 Vdc (Ground) external Gate input (TTL level, active high) Ground external Gate input Connect the module according the following schematics: AC Power Supply Adapter 5 Volts DC Serial Connecting Cable (for RS232) Fig.5: Connecting Diagram CPM module MP-RS232 (If not done already) Start terminal Program (e.g. Hyperterminal) Connect MPxxxRS module to 5 volts power supply (red: +5 volts, black: Ground) Ensure Linefeed (LF) is added to End of Line (EOL) of each line received (-> Set up of Terminal Program, fig. 3) Switch on 5 volts Power Supply Starting (default) conditions are: a) Communication Mode = 0x07 (response). b) Output Mode = 0x15 (counts, high voltage, Kill status, Gate status, temperature). c) Gate is activated. d) Kill is disabled. e) High Voltage = 0V. f) Counting time = 1000 msec. g) Pause time = 0 msec. h) Number of Counting intervals = infinite (0). Enter Command for Communication Mode (only if needed) SC[B] - Set Communication Mode Example: Command < SC7 > (bit 0, 1, 2 high) (See command overview) will echo: Enter Command for Output Mode (only if needed) SO[B] - Set Output Mode www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 8

Example: SO15 (bit 0, 1, 2, 3 high) (See command overview) Enter Kill disabling Kill function switches off high voltage power supply completely. (See command overview) SK0 Disables Kill Enable pre-adjusted High Voltage Set Voltage SV0 (when operating first time, voltage enable/disable needs to be set on a pre- defined status Set Voltage SV1 Pre-adjusted high voltage will be switched on Enter first command: SK0 (set kill 0) Enter second Command: SG0 (set gate 0) Enter third command: SV0 (set voltage 0) Enter fourth command: SV1 (set voltage 1) Enter fifth command: G (go) Counts: Highvoltage:Kill status: Gate Status; Inside Temperature Fig.: 6 Displayed echoed input commands and module s sent data after start of operating 5.2 Command Overview: Definitions Parameters between 0..65535 are represented by [nn] Parameters with only one digit are represented with [n] Parameters which are interpreted bitwise [B] Additional characters appended on a valid command are ignored. All normal commands are closed with return, except the commands of the counting mode. www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 9

Power on Conditions (default setting after switching power supply on) Communication Mode = 0x00 (no response). Output Mode = 0x00 (only counts). High Voltage = 0V. Gate is activated. Kill is activated. Counting time = 1000 msec. Pause time = 0 msec. Number = infinite (0). 5.2.1 The Adjustment Commands All adjustment commands start with S for SET SC[B] - Set Communication Mode The communication mode determines the information displayed on the screen entered by the operator. Bit 0: Module echoes incoming characters Bit 1: Module sends Prompt > when waiting for input Bit 2: Module responds acknowledge ACK or NACK after command. (Please enter only the decimal value!) Bit Setting for communication mode: Bit 2 1 0 Decimal Output: command 1 0 0 0 0 incoming characters are not echoed command 2 0 0 1 1 incoming characters are echoed 0 1 0 2 not defined command 3 0 1 1 3 Module sends Prompt > when waiting for input / with echo command 4 1 0 0 4 Module responds acknowledge ACK or NACK after command / no echo / no Promt command 5 1 0 1 5 Module responds acknowledge ACK or NACK after command / with echo command 6 1 1 0 6 Module responds acknowledge ACK or NACK after command / no echo/no Promt command 7 1 1 1 7 Module responds acknowledge ACK or NACK after command / with echo/with Promt Example: <SC7> <ENTER> will echo keyboard characters, output a Promt > while waiting for an input and finally responds with ACK for input command accepted or NACK for input command not accepted. SO[B] - Set Output Mode The Output mode determines the data displayed on the screen, sent by the module. Bit 0: Bit 1: Bit 2: Bit 3: Module adds High Voltage Value after each measurement. Module adds Kill status after each measurement. Module adds Gate status after each measurement. Module adds temperature after each measurement. If parameter = 15 (everything switched on), the counting time should be at least 12ms. (Only the decimal value is entered!) www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 10

Bit Setting for output (display) mode: Bit 3 2 1 0 Decimal Output command 1 0 0 0 0 0 Counts command 2 0 0 0 1 1 Counts / High Voltage command 3 0 0 1 0 2 Counts / Kill command 4 0 0 1 1 3 Counts / High Voltage / Kill command 5 0 1 0 0 4 Counts / Gate command 6 0 1 0 1 5 Counts / High Voltage / Gate command 7 0 1 1 0 6 Counts / Kill / Gate command 8 0 1 1 1 7 Counts / High Voltage / Kill / Gate command 9 1 0 0 0 8 Counts / Temp command 10 1 0 0 1 9 Counts / High Voltage / Temp command 11 1 0 1 0 10 Counts / Kill / Temp command 12 1 0 1 1 11 Counts / HV / Kill / Temp command 13 1 1 0 0 12 Counts / Gate / Temp command 14 1 1 0 1 13 Counts / HV / Gate / Temp command 15 1 1 1 0 14 Counts / Kill / Gate / Temp command 16 1 1 1 1 15 Counts / HV / Kill / Gate / Temp Example: <S07> <ENTER> will display the counts, adjusted high voltage value, the Kill status and the Gate status. SG[n] - Set Gate 0: deactivates the Gate function >0: activates Gate function SK[n] - Set Kill 0: deactivates the Kill function >0: activates Kill function SV[nn] - Set Voltage 0: set High Voltage of Module to 0 Volt and resets kill caused by over current >0: set the factory programmed High-Voltage value ST[nn] - Set Time nn: Counting time for counter in msec. Minimum counting time: 10 msec SP[nn] - Set Pause nn: Time between two measurements in msec. SN[nn] - Set Number 0: Module takes an infinite number of measurements until stop signal. nn: nn Measurements will be taken. www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 11

5.2.2 The Output Commands All Output commands start with D for DISPLAY DG - Display Gate 0: Gate deactivated. 1: Gate activated by Module. 2: Gate activated by external Signal (only when not activated by Module). (Optional available, not implemented by standard) DK - Display Kill 0: Kill disabled. 1: Kill activated by Module. 2: Kill activated by Module over current. DV - Display Voltage Output of High Voltage in V DT - Display Temperature Output of temperature in C DI - Display Information Output of following Module Information: Date of programming as day.mon.year. Module ID as Number. CPM Number as String. HV-Supply Number as Number. Hardware Revision as number.number. Software Revision as number.number. Over current limit as kilo counts/ sec. 5.2.3 The Counting Commands Counting Commands consist only of one character: G - Go The module enters counting mode and starts counting. The module sends the counts and additional values as set by command SO after each measurement. While counting, the module accepts only the below described commands. These commands are single character commands which are executed immediately without return. Also these commands produce no output echo. The Prompt will appear after leaving the counting mode when Bit 2 of Communication Mode is set. S Stop The module quits counting mode. G Gate Toggles Gate status of the module. K Kill Toggles Kill status of the module www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 12

6. In Case of Overcurrent Detection When light levels enter the Photocathode, generating more than the pre-adjusted max. count rate (5.5 Mcps), the internal Over-illumination protection circuit will activate the Kill function and all voltages are switched down to stand-by-mode (0 volts) In this case, the Kill output shows the status 2. How to re-activate counting mode? The Counting function can be re-activated only by entering: <SV0> (set voltage 0) <ENTER> <SV1> (set voltage1) <ENTER> 7. Power Supply Connection Pin 3 + 5V DC Pin 4 GND Coding Pin 1 NC Pin 2 NC Pin Signal Function Direction Comment 1 NC 2 NC 3 + 5V Power Supply Input 4 0 V Ground Input Fig. 7: Rear view of MPRS module, showing SUB D connector and four pin power supply connector 8. Dimensions 5V Power Supply Input Fig. 8: Outline dimensions of MPRS module www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 13

Module Type Dimension/mm A B C D E F G H I J K L M MPRS 9xx 4,5 36 4,5 33 127 120 30 20 19,5 10 18 45 45 MPRS 13xx 4,5 36 7 33 132 125 30 20 19 10 22,1 50 50 MPRS 19xx 4,5 36 7 33 137 130 30 20 19 10 22,1 50 50 Table 2: Dimensions of 9mm, 13mm and 19mm module type Warning: HIGH VOLTAGE This product operates at high voltage. Extreme care must be taken to ensure operator safety and to avoid damage to other instruments. Avoid direct contact with the CPM when high voltage is applied. Ensure that no light levels are applied, generating higher anode currents than specified. All given values are nominal/typical @ 20 C ambient temperature; specification subject to change without notice Warranty A standard 12-month warranty following shipment applies. Any warranty is null and void if the Module case has been opened. Your Partner of Choice Excelitas is a global technology leader focused on delivering innovative, customized solutions to meet the illumination, detection, and other high-performance technology needs of leading OEM customers worldwide. Our guiding principle - excellence is embodied in everything that we do. Simply stated, when we excel in all aspects of product performance, quality, reliability, delivery and service, we are enabling our OEM customers to excel as well. So contact us and put Excelitas expertise to work in your demanding applications. We will show how our innovations will help you deliver the perfect product. North America Customer Support Ctr. Excelitas Technologies Inc. 22001 Dumberry Road Vaudreuil, QC J7V 8P7 Canada Telephone: 1-450-424-3300 Fax: 1-450-424-3345 www.excelitas.com European Headquarters Excelitas Technologies Inc. Wenzel-Jaksch-Str. 31 65199 Wiesbaden, Germany Telephone: (+49) 611-492-247 Fax: (+49) 611-492-170 www.excelitas.com Asia Headquarters Excelitas Technologies Inc. 47 Ayer Rajah Crescent #06-12 Singapore 139947 Telephone: (+65) 6775-2022 Fax: (+65) 6775-1008 www.excelitas.com For a complete listing of our global offices, visit www.excelitas.com 2011 Excelitas Technologies, Inc. All rights reserved. The Excelitas logo and design are registered trademarks of Excelitas Technologies, Inc. All other trademarks not owned by Excelitas Technologies, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. Excelitas reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors. www.excelitas.com Rev 1.0 2011-02-28 M P-RS232 14