QUANTITATIVE IMAGING IN MULTICENTER CLINICAL TRIALS: PET



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Centers for Quantitative Imaging Excellence (CQIE) LEARNING MODULE QUANTITATIVE IMAGING IN MULTICENTER CLINICAL TRIALS: PET American College of Radiology Clinical Research Center v.1

Centers for Quantitative Imaging Excellence (CQIE) LEARNING MODULE This module is intended to provide a brief overview of general and PET-specific issues relevant to quantitative imaging for clinical trials. For additional information related to quantitative imaging for clinical trials, please see USEFUL LINKS on the CQIE web page (address provided below). For additional CQIE program information and qualification materials (MOP, forms, etc.), please refer to the CQIE web page. CQIE MOP and program information: www.acrin.org/nci-cqie.aspx After you have reviewed this module please let us know by submitting the learning module attestation. The link is provided at the end of this module.

What is Quantitative Imaging? As defined by the Toward Quantitative Imaging (TQI) task force of the Radiological Society of North America (RSNA): Quantitative imaging is the extraction of quantifiable features from medical images for the assessment of normal or the severity, degree of change, or status of a disease, injury, or chronic condition relative to normal. Quantitative imaging includes the development, standardization, and optimization of anatomical, functional, and molecular imaging acquisition protocols, data analysis, display methods, and reporting structures. These features permit the validation of accurately and precisely obtained image-derived metrics with anatomically and physiologically relevant parameters, including treatment response and outcome, and the use of such metrics in research and patient care. Buckler, et al., A Collaborative Enterprise for Multi-Stakeholder Participation in the Advancement of Quantitative Imaging, Radiology; Volume 258: Number 3, March 2011

What is Quantitative Imaging? Which imaging parameters are quantitative? Morphology Volume, 3D techniques (vct, vmr) Cellularity, density, composition of tissue Function Perfusion (DCE-MRI and DSC-MRI) Metabolic activity (PET) Molecule movement (DWI) MR Spectroscopy (MRS)

Quantitative Imaging as Biomarker What is an imaging biomarker? any anatomic, physiologic, biochemical, or molecular parameter detectable with one or more imaging methods used to help establish the presence and/or severity of disease. Ideally, a biomarker is an objectively measurable characteristic (versus a qualitative observation). Why use biomarkers? To speed the development of safe and effective medical therapies and procedures. Smith, et al., Biomarkers in Imaging: Realizing Radiology s Future, Radiology; Volume 227, Number 3, June 2003

Imaging Biomarkers in Clinical Trials Uses: Buckler, et al., A Collaborative Enterprise for Multi-Stakeholder Participation in the Advancement of Quantitative Imaging, Radiology; Volume 258: Number 3, March 2011

Imaging Biomarkers in Clinical Trials Needs: Standardization - the consistent performance of imaging, and adherence to protocols, for every research study performed at a given clinical site. Harmonization - the identification and implementation of mechanisms to control for inconsistencies of data between the different sites, particularly to ensure that imaging data generated with different systems are comparable. Facilitating the Use of Imaging Biomarkers in Therapeutic Clinical Trials, M.Graham, RSNA/SNM/FDA Two Topic Imaging Workshop, 2010

Imaging Standardization/Harmonization Why? Good Science Reliable decision making based on medical imaging requires comprehensive standards and tools to maintain integrity and ensure quality of results. For results to be of benefit to researchers and patients, the results must be accurate, comparable and reproducible (across patients, time-points, and institutions). Standardize imaging equipment (when possible) Standardize image/data acquisition Standardize image/data reconstruction Standardize image/data processing Standardize image interpretation

CQIE PET Qualification - Rationale Qualify NCI cancer centers in quantitative imaging methodologies: Static and dynamic PET, PET/CT (body and brain) Standardized qualification process and assessment, across 58 NCI-designation Cancer Centers Promote imaging standardization and harmonization within multi-center clinical trials.

CQIE PET - Qualification Imaging Clinical Test Cases 2 Brain, 2 Body- AC, NAC and CTAC files Anonymized and submitted via sftp Phantoms Uniform Cylinder- AC, NAC and CTAC files 2 bed position body FOV scan, using routine body protocol 1 bed position brain FOV scan, using routine brain protocol 1 bed position body FOV dynamic scan- 25 minute acquisition reconstructed in pre-defined time bins and summed ACR Phantom- AC NAC and CTAC files-don t forget to remove the 6 spheres and 6 mounting posts attached to the spheres 2 bed position body FOV scan, using routine body protocol 1 bed position brain FOV scan, using routine brain protocol Submitted via sftp

CQIE PET - Standardized QC Standardized QC helps to ensure the quantitative data generated within a clinical trial is comparable within an institution and across institutions. Sites are expected to incorporate the CQIE standardized QC measures into their existing QC activities. Sites are required to adhere to the CQIE standardized QC measures to maintain qualification status.

CQIE PET - Standardized QC QC is an important function of image quality and patient safety. Benefits of routine QC include Verification of operational integrity of imaging systems Early identification of technical issues Consistent, high image quality Consistent quantitative accuracy - SUV and Tracer Kinetics Quantitative imaging increases importance of QC. Participation in multicenter clinical research trials increases the importance of QC.

Standardized QC PET, PET/CT Test Purpose Frequency Physical Inspection To check gantry covers in tunnel and patient handling system. Daily Daily QC Check To test proper operation of scanner (per manufacturer s recommendations) Daily Normalization Uniformity Cross-Calibration Image quality To adjust system response to activity inside the field of view (FOV). To assess Transverse and Axial Uniformity across image planes by imaging a uniformly filled object. To monitor and identify discrepancies between the PET scanner and the dose calibrator. To check hot and cold spot image quality of standardized image quality phantom. As recommended by manufacturer (at least every 3 months); after software upgrades and after hardware service At lease every 3 months, after new setups and after software upgrades, At least every 3 months, after scanner upgrades, after new setups, and after modifications to the dose calibrator At least annually Documentation of the tests are subject to audit on annual renewal for qualification

Standardized QC - CT Test Standardized QC Tests for CT (as part of PET/CT) Water CT Number & Standard Deviation Artifacts Frequency Daily- Technologist Daily- Technologist Scout Prescription & Alignment Light Accuracy Imaged Slice Thickness-Slice Sensitivity Profile(SSP) Table Travel/Slice Positioning Accuracy Radiation Beam Width High-Contrast (Spatial) Resolution Low-Contrast Sensitivity and Resolution Image Uniformity and Noise CT Number Accuracy Artifact Evaluation Dosimetry/CTDI Annually Annually Annually Annually Annually Annually Annually Annually Annually Annually Documentation of the tests are subject to audit on annual renewal for qualification

Standardized QC Dose Calibrator Test Constancy (precision) Clock Accuracy Linearity Accuracy Geometry (position and volume) Frequency Each day of use and after equipment repair Daily Quarterly Annually and after equipment repair Upon installation and after replacement or repair of the chamber Accuracy of F18 Measurements Upon installation; at least once since June 2009 Documentation of the tests are subject to audit on annual renewal for qualification

Refresher - What is SUV? Standardized Uptake Value Semi-quantitative measure of the tracer uptake in a given region SUV Weight ConcTissue InjAct Weight Conc Tissue = Activity Concentration in Tissue (or region of a phantom) as measured in an ROI InjAct = Injected Activity at the start of the PET Emission acquisition Weight = Weight of patient (or active volume of phantom) Weight is the most common method of SUV normalization but factors like Lean Body Mass and Body Surface Area can also be employed

Technical Factors Affecting SUV Strict adherence to research protocol Accurate recording of time, activity and weight Dose calibrator accuracy, constancy, low background Scanner normalization, calibration Image reconstruction algorithm PET corrections: attenuation, scatter, randoms, decay ROI definition/methods for SUV SUV Normalization factor (body weight, body surface, lean body mass)

Quantitative Accuracy With so many factors contributing to the accuracy of SUV calculation it is of the utmost importance to ensure all instruments are functioning within acceptable limits at all times. All times must be recorded to the minute and all clocks must agree with the PET scanner clock to the minute. Assay Time, Injection Time, Residual Assay Time, Scan Start Time.

Strict Adherence to Protocol Deviation from protocol invalidates quantitative data. Increase or decrease in circulation time alters SUV Blood Glucose and Insulin levels alter SUV when using some tracers Patient behavior before and during uptake period can affect tracer distribution and SUV Moving the patient between scans when instructed not to may interfere with or invalidate core lab analysis Failure to perform additional scans with IV contrast may interfere with or invalidate core lab analysis

Accurate Data Entry If weight, net injected dose, time of dose assay or injection are recorded or entered incorrectly, any SUV calculated for the resulting images will be incorrect Good Research Practice Weigh every person on the scale in the PET Facility; do not take the patient s word Ensure all clocks in the PET Facility are synchronized to the PET scanner clock and to the minute

Dose Calibrator The dose calibrator must be maintained and checked in accordance with NRC guidelines The dose calibrator clock must be in agreement with the PET scanner clock to the minute All doses must be assayed with the dose calibrator background at 0 Remove all radioactive sources from the area Check to ensure background is 0 before and after the assay of every dose Keep a spare dose calibrator dip stick handy at all times for use in the event of contamination Clean even small amounts of radioactive material from the chamber if detected If background above 0 is unavoidable record the background reading and the time measured before and after dose assay

Image Reconstruction Algorithm Changing the reconstruction algorithm can affect the activity distribution visualized in the final image In all research trials, it is important to use the same reconstruction algorithm with the same parameters for all patients This will help ensure data across patients and time points can be compared both qualitatively and quantitatively

PET Corrections Normalization Corrects for non-uniformities in detector response Attenuation Corrects for decrease in detected events due to absorption and scatter Scatter Corrects for detected events that underwent a scattering event Randoms Corrects for detected events that originated from two different annihilation events DeadTime Corrects for a reduction is detected counts caused by elevated count rate Decay Corrects for the decreased activity during the scan due to radioactive decay. Cross-Calibration/SUV Converts the reconstructed counts into concentration (or SUV) in the final image

Scanner Normalization Corrects for nonuniformities in event detection over the full scanner Sources of nonuniformities: Variation in amount of scintillation light collected due to crystal nonuniformities and detector design (detector effect) Difference in detection sensitivity due to angle of incidence Most manufacturers use a uniform cylinder acquisition to generate the normalization correction Uniform cylinder

Routine Scanner QC Purpose of scanner QC is to ensure that the system performing as expected and expose any issue before they have a clinical impact QC program should involve daily, weekly, monthly, quarterly and annual tests QC programs should be reviewed regularly and updated should any deficiencies be found Scanner QC helps ensure that resulting images are quantitatively reliable

ROI Definition How the boundaries of an ROI are defined affects quantitative results A short Example: Max Max Pixel in ROI Peak Avg. in 1 cm ROI CT Avg. in ROI using boundaries defined on CT To the right: Actual Diameter is 16 mm Max = 2.110 Peak = 1.721 CT = 1.538

Normalization Factor-Body Weight It is essential to accurately record patient weight Ensure your scale is accurate Never record weight as reported by the patient or test subject.

Important Points Quantitative, multi-center clinical trials require reliable, quantitatively accurate image data This is obtained by properly maintaining all imaging related equipment, strictly following the research protocol, and accurately recording all required patient related information A small number of non-compliant cases can invalidate an entire trial

Imaging & Research-Related Links Quantitative Imaging Biomarkers Alliance, http://www.rsna.org/research/qiba.cfm RSNA/ACRIN Imaging Researchers Workshop (Sept. 2011), http://www2.rsna.org/re/ctsaiwgacrin2011/index.htm

Please follow the link below to report your review of this module https://www.surveymonkey.com/s/cqie_quantitativeimaging_pet CQIE PET questions should be directed to CQIE-PET@acr.org American College of Radiology Clinical Research Center 1818 Market Street - Suite 1600 Philadelphia, PA 19103 Thank you!