YMC GENERAL CATALOG Reversed-Phase C8 Columns ( Types and characteristics of C8 packing materials Guides for selecting C8 packing materials YMC-Triart C8 YMC-UltraHT series YMC-UltraHT series YMC-UltraHT Pro C8 YMC-UltraHT Hydrosphere C8 Pro series Features of Pro series ODS columns YMC-Pack Pro C8 Hydrosphere C8 YMC-Pack Pro C8 RS YMC-Pack ODS series YMC-Pack ODS-A YMC-Pack ODS-AM YMC-Pack ODS-AQ YMC-Pack ODS-AL J, sphere ODS series ODS-H8M8L8 YMC-Pack PolymerC8, 7 8 9,,7 8,9,,, 第 章.indd..8 :9: AM
Types and characteristics of C8 packing materials Octadecyl-silica ( is the industry standard packing material for HPLC applications. YMC Co., Ltd. offers packing materials with an impressive assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety. We also offer hybrid-silica based C8 packing materials that are widely applicable to a variety of fields, from microanalysis to large-scale isolation. Types of C8 packing materials Base Hybrid silica Silica Polymer Products YMC-Triart C8 YMC-UltraHT series YMC-Pack Pro C8 Hydrosphere C8 YMC-Pack Pro C8 RS YMC-Pack ODS series J sphere ODS series YMC-Pack PolymerC8 Reversed-Phase C8 Columns( Types and characteristics of C8 ( packing materials YMC-Triart C8 UltraHT series Pro series Product name YMC-Pack ODS series Pore size (Å) Particle size ODS C% Silanol treatment Usable ph range Characteristics Pages Yes Versatile hybrid silica based ODS column.. Great chemical durability Excellent peak shape in every conditions µm-ods column for Ultrafast Pro C8 LC method Superior separation of basic Yes. 8. compounds Superior separation of Hydrosphere C8 hydrophilic compounds Reduced analysis time while 7 maintaining resolution Processed with YMC Co., Ltd. s Pro C8 Yes advanced endcapping technology Superior separation of basic compounds Hydrosphere C8. 8. Superior separation of hydrophilic compounds Yes 9 Can be used with % water mobile phase Highly durable ODS Pro C8 RS 8 Yes.. Superior separation of basic compounds and hydrophobic compounds 7 Currently in use worldwide, ODS-A Yes ODS with wide pore size For separation of peptides 7 and proteins, ODS-AM. 7. 7 Yes Outstanding lot-to-lot reproducibility,7 Superior separation of ODS-AQ Yes 8,9 hydrophilic compounds ODS-AL 7 No For separation utilizing residual silanol, J'sphere ODS series ODS-H8 8 Yes. 9. High carbon ODS ODS-M8 8 Yes Medium carbon ODS,. 7. ODS-L8 8 9 Yes Low carbon ODS YMC-Pack PolymerC8.. Polymer-based ODS, 第 章.indd..8 :9: AM
Guides for selecting C8 packing materials YMC Co., Ltd. offers twelve types of ODS columns, each with unique separation characteristics. The proper selection of packing material is therefore a key factor in the establishment of efficient separation conditions. Selection of packing materials without sufficient consideration of the separation characteristics may result in an expense of much time and effort in separating a target material. YMC-Triart C8 YMC-Pack Pro C8 YMC-Pack ODS-A J sphere ODS-M8 Reversed-Phase C8 Columns( YMC-Pack Pro C8 RS J sphere ODS-H8 YMC-Pack ODS-A (Wide-Pore) J sphere ODS-L8 Hydrosphere C8 YMC-Pack Pro C8 RS YMC-Pack ODS-AL J sphere ODS-H8 J sphere ODS-L8 YMC-Pack PolymerC8 YMC-Triart C8 YMC-Pack Pro C8 Hydrosphere C8 YMC-Pack Pro C8 RS YMC-Pack ODS-AM YMC-Pack PolymerC8 YMC-Triart C8 YMC-Pack Pro C8 Hydrosphere C8 YMC-Pack Pro C8 RS YMC-Pack ODS-AM YMC-Pack PolymerC8 Hydrosphere C8 YMC-Pack ODS-AQ J sphere ODS-L8 YMC-Pack ODS-A (Wide-Pore) YMC-Triart C8 YMC-Pack Pro C8 RS J sphere ODS-H8 YMC-Pack PolymerC8 第 章.indd..8 :9: AM
Versatile Hybrid Silica Based HPLC Column YMC-Triart C8 YMC-Triart C8 is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance. YMC-Triart C8 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation and surface modification. Rugged, long lasting, hybrid particle technology Integration of triple artistic technologies Features Benefit Hybrid silica base Usable over a wide ph range makes the column excellent for method development. Reversed-Phase C8 Columns( Great chemical durability Lower back pressure Excellent resolution Long column lifetime over wide ph range. Low cost per analysis. Ability to use on both older and newer instrumentation. Rugged, first choice option for analysis of acids, bases and neutral compounds. Specifications Base :organic/inorganic hybrid silica Stationary :C8 (as USP-L) Particle : µm, µm, µm Pore : Å Carbon % :% Bonding :polymeric type Endcapping :multi-stage endcapping ph range :ph YMC-Triart C8 -Integration of triple artistic technologies- <>Versatile hybrid material YMC-Triart C8 is a multi-layer hybrid particle consisting of two types of layers: a silica based layer (inorganic) and a hybrid polymer based layer (organic). This layered structure contributes to Triart's combined physical durability and chromatographic performance. Particle synthesis by Microreaction inorganic-silane layer Versatile hybrid silica material Multi-stage endcapping Image structure for hybrid-silica gel organic-silane layer 第 章.indd..8 :9:8 AM
YMC-Triart C8 -Integration of triple artistic technologies- <>New granulation process by microreactor technology Homogeneous and uniform particle Triart C8 Narrow pore distribution d Vp/d logd Hybrid particle for Triart Silica particle for conventional product Pore size (Å) Hybrid particle for competitor's product Reversed-Phase C8 Columns( Use of microreactor technology produces homogeneous and uniform particles that results in low operating pressure and very reproducible surface modification making for the ultimate in column-to-column and lot-to-lot reproducibility. YMC-Triart C8 has a very narrow pore size distribution that enables narrow and reproducible peak shapes for analytes. <>Surface modification technology Multi-stage endcapping Endcapping methods employed for production of conventional products typically employ a single endcapping compound in one chemical step to "neutralize" highly reactive silanols. Unfortunately, highly reactive silanols are easily hydrolyzed and contribute to poor durability. Additionally, low reactive silanols endcap stubbornly, producing a surface modification that is often non-reproducible, and leading to peak tailing. YMC-Triart C8 solves this problem by using an innovative, newly developed "measured multi-stage/multicompound surface modification" technology which results in the thorough endcapping of all silanols that are accessible to analytes. Reaction image for "multi-stage endcapping" Evaluation of silanol activity mau Before EC STEP STEP.... Q998C min Silanol group Endcapping (EC) group mau STEP Peak tailing for basic compounds.... Q998E min Column : µm,. mmi.d. Eluent : mm KHPO-KHPO (ph.9)/acetonitrile (/) Flow rate :. ml/min Temperature : Detection :UV at nm Sample :. Chlorpheniramine. Dextromethorphan. Propyl p-hydroxybenzoate 7 第 章.indd 7..8 :9: AM
Versatile Hybrid Silica Based HPLC Column YMC-Triart C8 Quality Control -Excellent reproducibility- Packing Material Basic compounds YMC-Triart C8 exhibits excellent lot-to-lot reproducibility for all types of compounds including difficult to chromatograph molecules that exhibit basic and coordination compound behavior. Coordination compounds Lot. E Lot. E Lot. D Lot. D Lot. C Lot. C Lot. B Lot. B.. J9Y. 7.... 7. min Lot. A.. J9X.. 8.... min Lot. A. Chlorpheniramine. Propyl p-hydroxybenzoate. Dextromethorphan. Hinokitiol. Methyl benzoate Reversed-Phase C8 Columns( Column : µm,. mmi.d. Eluent : mm KHPO-KHPO (ph.9)/acetonitrile (/) Flow rate :. ml/min Temperature : Detection :UV at nm Column : µm,. mmi.d. Eluent :acetonitrile/.% HPO (/) Flow rate :. ml/min Temperature : Detection :UV at nm Packed Column Rigid control of theoretical plate number (N) and tailing factor (Tf) are exhibited for each YMC-Triart C8 packed column. Theoretical plate number. Theoretical plate number. Tailing factor. Column number.. Tailing factor Column : µm,. mmi.d. Eluent :acetonitrile/water (/) Flow rate :. ml/min Temperature :ambient Sample :butyl benzoate Low back-pressure -Free from pressure problem- Column pressure and solvents Standard ODS -Best balance hydrophobicity- Comparison of separation selectivity.8 Column pressure (MPa) 8 8 7 8 9 Organic solvent rate (%) Column : µm,. mmi.d. Eluent :acetonitrile/water or methanol/water Flow rate :. ml/min Temperature : Triart C8, MeOH/W Triart C8, ACN/W Pro C8, MeOH/W Pro C8, ACN/W Hydrogen-bonding capacity (caffeine/benzene).....8. Hydrosphere C8 CAPCELL PAK C8 AQ Gemini C8 Pro C8 Unison UK-C8 Inertsil ODS- Hypersil GOLD CAPCELL PAK C8 MGII Gemini NX-C8 SunFire XTerra MS C8 XBridge C8 Inertsil ODS- Eternity-C8 Mightysil RP-8 C8 L-column ODS Triart C8 TSKgel ODS-S Develosil C-UG Atlantis T CAPCELL PAK C8 MGIII Cadenza CD-C8 L-column ODS Standard ODS The most versatile columns as first-choice. Eluent : MeOH/W (8/) [amylbenzene], MeOH/W (/7) [caffeine, benzene].... 8.... (S98A) Hydrophobicity k (amylbenzene) Pro C8 RS Develosil ODS-SR YMC-Triart C8 is designed for use in all conditions. Even with higher viscosity methanol, YMC-Triart C8 can operate with lower pressure (typically % less than conventional columns). Conventional hybrid silica based ODS columns tend to be less hydrophobic than silica based columns. YMC-Triart C8 has a favorable carbon balance and may be used as a "versatile firstchoice" method development column. 8 第 章.indd 8..8 :9:7 AM
Great durability -Wide usable ph range and long lifetime- Durability in high ph Phosphate buffer (ph., ) Rate of theoretical plate number % % 8% % % % % Silica based C8 YMC-Triart C8 Hybrid-silica based C8 Time (hr) Triethylamine (ph., ) Rate of theoretical plate number % % YMC-Triart C8 8% % % Silica based C8 % % Time (hr) Column : µm,. mmi.d. Eluent : mm KHPO-KPO (ph.)/methanol (9/) Flow rate :. ml/min Temperature : Sample :benzyl alcohol Column : µm,. mmi.d. Eluent : mm triethylamine (ph.)/methanol (9/) Flow rate :. ml/min Temperature : Sample :benzyl alcohol Reversed-Phase C8 Columns( Durability in high temperature ph.9, 7 ph, 7 Rate of theoretical plate number % % 8% % % % Silica based C8 YMC-Triart C8 Silica based C8 alkaline-resistant type % % 7 8 Time (hr) Time (hr) Rate of retention time % % 8% % % % YMC-Triart C8 Silica based C8 Hybrid-silica based C8 The columns are kept in acetonitrile/water/tfa (/9/), 7 C, and tested for performance every hours. Column : µm,. mmi.d. Eluent : mm KHPO-KHPO (ph.9)/acetonitrile (9/) Flow rate :. ml/min Temperature :7 Sample :phenol Test conditions Column : µm,. mmi.d. Eluent :acetonitrile/water (/) Flow rate :. ml/min Temperature :7 Sample :butyl benzoate YMC-Triart C8 shows great chemical stability provided by newly developed hybrid silica. Even in high ph or high temperature conditions, the lifetime of YMC-Triart C8 is more than times greater than conventional ODS columns. Reduced column bleed -Operate at high sensitivity- Bleed test by LC/MS.E+9 Scan ES+ TIC Triart C8 No column 8.E+8.E+8.E+ 8 min Column : µm,. mmi.d. Eluent : A) water/formic acid (/.) B) acetonitrile/formic acid (/.) % B (- min), -% B (- min), % B (- min), -% B (-. min), % B (.-. min) Flow rate :. ml/min Temperature : Detection :ESI positive, TIC (Mass Range: -) Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection techniques. In the example cited above, use of a. mmi.d. YMC-Triart C8 column shows no contribution to the signal observed by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques that are sensitive to particle degradation. 9 第 章.indd 9..8 :9: AM
Versatile Hybrid Silica Based HPLC Column YMC-Triart C8 Excellent resolution -Without adsorption and tailing- Analysis of basic compounds YMC-Triart C8 µm,. mmi.d. Q998C Silica based C8 Brand D µm,. mmi.d. Ingredients in a cough/cold medication. HC. HC CH N N Chlorpheniramine O Cl OH. N CH H O Dextromethorphan CH Reversed-Phase C8 Columns( Silica based C8 Brand N µm,. mmi.d. Hybrid-silica based C8 Brand I8 µm,. mmi.d.... 7.... 7. min Q98C J9K Q9F O Propyl p-hydroxybenzoate Column :. mmi.d. or. mmi.d. Eluent : mm KHPO-KHPO (ph.9)/acetonitrile (/) Flow rate :. ml/min for. mmi.d.. ml/min for. mmi.d. Temperature : Detection :UV at nm YMC-Triart C8's innovative surface modification technology results in excellent peak shapes even for the basic compounds that often exhibit tailing shapes on conventional silica and hybrid silica based ODS columns. Analysis of coordination compounds Hinokitiol CH. CH. O O CH YMC-Triart C8 µm,. mmi.d. OH O Q99G Hinokitiol Methyl benzoate Silica based C8 Brand N µm,. mmi.d. Hybrid-silica based C8 Brand I8 µm,. mmi.d..... 8.... min J9B Q9A Column :. mmi.d. or. mmi.d. Eluent :acetonitrile/.% HPO (/) Flow rate :. ml/min for. mmi.d.. ml/min for. mmi.d. Temperature : Detection :UV at nm YMC-Triart C8 has an extremely low level of metal impurities, much lower than conventional products. YMC-Triart C8 is able to provide excellent peak shape for coordination compounds. Analysis of acidic compounds YMC-Triart C8 µm,. mmi.d. Organic acid. Formic acid. Acetic acid. Propionic acid Hybrid-silica based C8 Brand G µm,. mmi.d............... 7. 7. min Q9C Q977A Column :. mmi.d. or. mmi.d. Eluent :acetonitrile/.% HPO (/9) Flow rate :. ml/min for. mmi.d.. ml/min for. mmi.d. Temperature :7 Detection :UV at nm YMC-Triart C8 is synthesized utilizing methodology borrowed from microreactor technology. This synthesis technique allows for a reduction of impurities that contribute to peak tailing during the analysis of some types of acidic compounds. 第 章.indd..8 :9: AM
Excellent versatility -Buffer independency- Comparison of clemastine analysis with conventional columns mm phosphate buffer (ph.7)/organic solvent mm CHCOONH/organic solvent methanol acetonitrile methanol acetonitrile YMC-Triart C8. mmi.d. Tf.97 Tf. Tf. Tf. F988H7 F988I7 F988F7 F987G7 Silica based C8 Brand C. mmi.d. Tf. F977H7 Tf. F977I7 Tf. F977F7 Tf. F98G7 Silica based C8 Brand D. mmi.d. Tf.9 F977H7 Tf.8 F977I7 Tf. F97F7 Tf. F97G7 Reversed-Phase C8 Columns( Silica based C8 Brand I. mmi.d. Tf. Tf.87 Tf. Tf.88 F97H7 F97I7 F97F7 F97G7 Hybrid-silica based C8 Brand I8. mmi.d. Tf. Tf.88 Tf. Tf.78 F97H7 F97I7 F97F7 F97G7... min. 7. 8. min 8. 9.. min. 7. 8. min HC N O Clemastine CH Cl Column : µm,. mmi.d. or. mmi.d. Eluent : A) mm KHPO-KHPO (ph.7) or mm CHCOONH (ph.7) B) methanol or acetonitrile -9% B (- min), 9% B (- min) Flow rate :. ml/min Temperature : Detection :UV at nm Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C8 can separate clemastine sharply using many buffer/solvent combinations. Improvement of loadability -Minimizing strong solvent/sample loading effects- Influence on peak shapes by injection volume YMC-Triart C8 solvent for sample injection volume. µl. µl. µl. µl Hybrid-silica based C8 Silica based C8 Column : µm,. mmi.d. or. mmi.d. Eluent : A) water/formic acid (/.) B) acetonitrile/formic acid (/.) % B(-. min), -% B (.-. min) Flow rate :. ml/min Temperature : Detection :UV at 7 nm Peak anomaly at. µl.. OCHCHCHNHCH(CH) OH HCO HCO CN CH C (CH)NCHCH OCH OCH CH(CH)... min Propranolol ( µg/ml) Verapamil ( µg/ml) YMC-Triart C8 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of organic solvent for solubilization prior to analysis. 第 章.indd..8 :9:7 AM
Versatile Hybrid Silica Based HPLC Column YMC-Triart C8 Fast analysis of paraquat and diquat mau %B,. ml/min,.7 MPa mau %B,. ml/min,. MPa Insufficient separation Eluent optimization Baseline separation, But long analysis time R9J 8 min R97C min Downsizing Reversed-Phase C8 Columns(.. N + N + Br HC N + N+ Diquat Paraquat CH Cl mau %B,. ml/min,. MPa 8% reduction in run time Eluent : A) water/hfba* (/.) B) acetonitrile/hfba* (/.) Temperature :7 Detection :UV at 9 nm Injection : µl (. mg/ml) *heptafluorobutyric acid R9B min A p p l i c a t i o n (Q9E) Anthocyanin mau 8 8. Delphinidin--O-galactoside. Delphinidin--O-glucoside. Cyanidin--O-galactoside. Delphinidin--O-arabinoside. Cyanidin--O-glucoside. Petunidin--O-galactoside 7. Cyanidin--O-arabinoside 8. Petunidin--O-glucoside 9. Peonidin--O-galactoside. Petunidin--O-arabinoside. Delphinidin. Peonidin--O-glucoside. Malvidin--O-galactoside. Peonidin--O-arabinoside. Malvidin--O-glucoside. Malvidin--O-arabinoside 7. Cyanidin 8. Petunidin 9. Peonidin. Malvidin 7 9 7 8 9 min Column : YMC-Triart C8 ( µm, Å). mmi.d. Eluent : A) water/formic acid (9/) B) acetonitrile/methanol/water/formic acid (././/) -8% B (- min), 8-7% B (- min), % B (- min) Flow rate :. ml/min Temperature : Detection :UV/VIS at nm Sample :commercial bilberry powder (. mg/ml) 第 章.indd..8 :9:8 AM
A p p l i c a t i o n (R99C) Peptide mau 7. Oxytocin (MW,7). Met-Enkephalin (MW 7). Leu-Enkephalin (MW ). Neurotensin (MW,7). γ-endorphin (MW,89). β-endorphin (MW,) min Column : YMC-Triart C8 ( µm, Å). mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -% B (- min) Flow rate :. ml/min Temperature :7 Detection :UV at nm A p p l i c a t i o n Intens. x Mixture ppb LC/MS analysis for Triazolam and Etizolam.. H C N H C N N N N N S (SA) Cl N H C N 8 Time [min] ng_ml_rb 9.d: EIC.±. +All MS ng_ml_rb 9.d: EIC.78±. +All MS Courtesy of J.Watanabe, Bruker Daltonics K. K. Cl Cl Triazolam (MW.) Etizolam (MW.8) Column : YMC-Triart C8 ( µm, Å). mmi.d. Eluent : A) mm HCOOH, B) acetonitrile -% B (- min), % B (- min) Flow rate :. ml/min Temperature : Detection :ESI, positive mode (Bruker Daltonics microtof) Injection : µl Reversed-Phase C8 Columns( Ordering information Analytical columns Pore size (Å) S-. TAS-WT. TAS-WT. TAS-WT. 7 TAS-LWT. TAS-WT. TAS-WT. TAS-WT. TAS-WT. TAS-WT. TAS-HWT. TAS-WT. 7 TAS-LWT. TAS-WT. TAS-WT S-. TAS-WT. TAS-WT. TAS-WT. 7 TAS-LWT. TAS-WT. TAS-WT. TAS-WT. 7 TAS-LWT. TAS-WT. TAS-WT. TAS-WT. 7 TAS-LWT. TAS-WT. TAS-WT. TAS-WT Please inquire for µm columns. Semi-preparative columns Pore size (Å) S- TAS-WT TAS-WT TAS-WT TAS-WT TAS-WT Semi-preparative columns YMC-Actus Triart C8 S- TAS-WX TAS-WX TAS-WX 7 TAS-LWX TAS-WX See P8- for details of YMC-Actus series. Guard cartridge columns (with inner diameter. mm: /pack,. mm: /pack) S-. TAS-CC. TAS-GCC S-. TAS-CC. TAS-GCC A cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) XPCHSMW XPCHW Guard columns S- TAS-WTG TAS-WTG 第 章.indd..8 :9: AM
Ultra-Fast LC column YMC-UltraHT YMC-UltraHT series is a C8 bonded-phase based on highly efficient µm spherical silica particles. YMC- UltraHT series columns are specifically designed for high-speed and high throughput analysis in various fields such as pharmaceuticals, foods, and environmentals. YMC-UltraHT series has two products. Pro C8 as our standard ODS, and Hydrosphere C8 for hydrophilic compounds. Both of them show the same selectivity and superior peak shapes as µm and µm Pro series, allowing for an easy method transfer from conventional HPLC to ultra-fast HPLC without changing elution conditions. µm ODS column ideal for Ultra-Fast LC Features Reversed-Phase C8 Columns( Superior column performance at higher flow rate and higher pressure Reduced analysis time while maintaining excellent resolution Excellent resolution with back pressure less than that of sub- µm Applicable to both conventional HPLC and specific ultra pressure LC system Same selectivity and superior peak shapes as µm and µm Pro series Simple method transfer from conventional HPLC on Pro series; no need to change elution conditions Characteristics of µm packing material HETP (mm/plates). (. ml/min for mmi.d.) (.-.8 ml/min for mmi.d.) YMC µm. YMC µm. Brand L.8 µm YMC µm. Brand I7.7 µm. 7 8 9 Interstitial linear velocity (mm/sec) Column pressure (MPa) 8 7 (.-.8 ml/min for mmi.d.) Brand I7.7 µm Brand L.8 µm YMC µm YMC µm YMC µm 7 8 9 Interstitial linear velocity (mm/sec) YMC-UltraHT series shows nearly the same efficiency as sub- µm columns for ultra fast LC system and reduces the column pressure significantly. Column :. or. mmi.d. Eluent : acetonitrile/water (/) Temperature : Sample : Butyl benzoate YMC-UltraHT series Pro C8 Hydrosphere C8 Particle Size Pore Size (Å) Specific Surface area (m /g) Carbon Content % % Bonding Type Monomeric Monomeric Available ph Range - 8-8 Characteristics Standard type For polar analytes 第 章.indd..8 :9: AM
Ultra Fast analysis for flavonoids. ml/min µl injection 8. MPa tr(7). min N(7) 77, /m 9% Decrease in analysis time 9% Increase in efficiency tr(7).7 min N(7), /m Flavonoids. Myricetin. Quercetin. Apigenin. Kaempferol. Baicalein. Chrysin 7. Acacetin Reversed-Phase C8 Columns(. ml/min µl injection MPa Eluent : acetonitrile/water/formic acid (//.) Temperature : Detection : UV at nm This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT column. Since the column efficiency is not decreased even when the flow rate is times as fast as the conventional condition, it can be shorten analysis time by 9% without losing the separation characteristics. Applicable for conventional LC system mmi.d. mau 8 (N7B) min N() = N() = N() = 7 N() = 78 Rs(, ) =. mau (N7E) min Flow rate :.8 ml/min Injection : µl Flow rate :.8 ml/min Injection : µl N() = N() = 8 N() = N() = 8 Rs(, ) =. mmi.d. mau 8 (N78E) min N() = 7 N() = N() = N() = 9 Rs(, ) =. Flow rate :. ml/min Injection : µl mau (N78B) Flow rate :. ml/min Injection : µl min N() = N() = N() = N() = Rs(, ) =.9 YMC-UltraHT series can be used for fast analysis with conventional LC system, as its pressure is lower than commercial sub- µm columns. Considering the extra column band broadening, the column of. mml.d. is more applicable for conventional LC system than the column of. mml.d.. Cephalosporin antibiotics. Cephalexin. Cefaclor. Cephaloglycin. Cephaloridine Column Eluent :YMC-UltraHT Pro C8 :acetonitrile/ mm KHPO (/9) Temperature :7 Detection :UV at nm 第 章.indd..8 :9: AM
Reversed-Phase C8 Columns( Analytical columns YMC-UltraHT Pro C8 Superior column performance at higher flow rate and higher pressure Excellent resolution with back pressure less than % of commercial sub- µm Same selectivity and superior peak shapes as µm and µm Pro C8 Simple method transfer from conventional HPLC Pore size: Å Carbon content:% Usable ph range:. 8. F i r s t c h o i c e O D S c o l u m n f o r U l t r a - f a s t L C YMC-UltraHT Pro C8 is a C8 bonded-phase based on highly efficient µm spherical silica particles. YMC- UltraHT Pro C8 is designed for high-speed and high throughput analysis, advanced endcapping technology makes YMC-UltraHT Pro C8 highly appropriate for analysis of basic compounds. The physical properties and separation characteristics are strictly controlled, so it is highly suitable as the first choice column for ultra fast LC. Excellent peak shapes mv N(),/m Tf().7 MPa mv N() 89,/m Tf().99 9 MPa mv N() 78,/m Tf(). 7 MPa...8... [min]...8... [min]...8... [min] YMC-UltraHT Pro C8 exhibits greater peak shapes of alkaloids, which are highly basic compounds compared to commercial silica based sub- µm columns. Alkaloids. Scopolamine. Atropine. Cinchonine. Quinine. Dihydroquinine Column :. or. mmi.d. Eluent : mm CHCOOH-CHCOONH (ph.9)/ acetonitrile (8/) Flow rate :. ml/min Temperature : Detection : UV at nm Peptide mapping mv 8 %B (- min) -%B (- min) %B (- min) mv (8.D) min %B (-. min) -%B (.-. min) %B (.- min) Analysis of a tryptic digest of cytochrome C is shown below. A mm length UltraHT Pro C8 column of µm particle can be shorten the analysis time by one-thirds compared to a mm column packed with µm Pro C8. undigested Cytochrome c N8I min Eluent :A) acetonitrile/water/tfa (/9/.) B) acetonitrile/water/tfa (//.) Flow rate :. ml/min Temperature :7 Detection :UV at nm Sample :tryptic degest of Cytochrome c Ordering information Analytical columns Pore size (Å) S-. ASS-WT. ASS-WT. 7 ASS-LWT. ASS-WT. ASS-WT. 7 ASS-LWT. ASS-WT 第 章.indd..8 :: AM
Analytical columns YMC-UltraHT Hydrosphere C8 Can be used with % aqueous as mobile phase Superior column performance at higher flow rate and higher pressure Reduced analysis time while maintaining excellent resolution Pore size: Å Carbon content:% Usable ph range:. 8. T h e b e s t c o l u m n f o r h y d r o p h i l i c c o m p o u n d s YMC-UltraHT Hydrosphere C8 is a C8 bonded phase based on highly efficient µm spherical silica particles. It is designed to maintain hydrohilicity on the packing surface for superior separation of hydrophilic compounds. Ultrafast analyses of soy isoflavones 8 min 7 7 8 8 7 8 9 9 9 Rs(,)=.8 Rs(7,8)=. Rs(,)=.9 Rs(,)=.9.-% B (- min). ml/min µl injection.-% B (- min). ml/min.-% ACN (- min). µl injection. ml/min MPa Examples illustrated shows the optimization process of isoflavones separation by µm YMC-UltraHT Hydrosphere C8. It has the same selectivity as µm Hydrosphere C8, so there is no need to change the condition at downsizing. Maintaining resolution, the analysis time can be reduced by using µm packing material. Reversed-Phase C8 Columns( 8 min min Rs(,)=. Rs(,)=. Rs(7,8)=.7 9.-% B (-. min) 9.-%.9 ml/minacn (-. min) 7 8.9. ml/min µl injection 7 8 MPa Soy isoflavones. Daidzin. Glycitin. Genistin. "-O-Malonyldaidzin. "-O-Malonylglycitin. "-O-Acetyldaidzin 7. "-O-Acetylglycitin 8. "-O-Malonylgenistin 9. Daidzein. Glycitein. "-O-Acetylgenistin. Genistein. min. min Eluent :A) water/acetic acid (/) B) acetonitrile/acetic acid (/) Temperature : Detection :UV at nm Ordering information Analytical columns Pore size (Å) S-. HSS-WT. HSS-WT. 7 HSS-LWT. HSS-WT. HSS-WT. 7 HSS-LWT. HSS-WT 7 第 章.indd 7..8 :: AM
High performance reversed-phase column Features of Pro series ODS columns Pro series high performance reversed-phase columns feature advanced endcapping technology and quality assurance which is ready for validation. The Pro series includes three kinds of ODS, each with unique separation characteristics. We provide products with excellent performance and stable quality all over the world to fulfill the needs of the pharmaceutical industry, in which production, as well as research and development, are increasingly globalized. Providing products with stable quality all over the world Feature Reversed-Phase C8 Columns( Silica gel base, low in metal impurities Processed with advanced endcapping technology Superior separation of basic compounds Useful for LC/MS Excellent durability Excellent reproducibility Two types of test reports are included with each column YMC-Pack Pro C8 Hydrosphere C8 YMC-Pack Pro C8 RS Types of Pro series ODS Pro C8 Hydrosphere C8 Pro C8 RS Particle Size,,,,,, Pore Size (Å) 8 Specific Surface area (m /g) Carbon Content % % % Bonding type monomeric monomeric polymeric Available ph Range - 8-8 - Characteristics Standard type Separation for hydrophilic compounds High durability 8 第 章.indd 8..8 :: AM
Three kinds of ODS, each with different separation characteristics The three kinds of ODS columns in the Pro series each have unique carbon content and separation characteristics, including hydrophobicity, plane recognition ability, and hydrogen-bonding capacity. Selection according to the analyte and mobile phase of Pro C8 with standard hydrophobicity, Pro C8 RS with high hydrophobicity, or Hydrosphere C8 with low hydrophobicity, facilitates method development. Hydrophobicity and plane recognition ability of Pro series ODS Reversed-Phase C8 Columns( min min min. Uracil. n-butylbenzene. o-terphenyl. n-amylbenzene. Triphenylene :. mmi.d. Eluent :methanol/water (8/) Flow rate :. ml/min Temperature :7 Detection :UV at nm Comparison of hydrophobicity and hydrogen-bonding capacity of various columns Hydrogen-bonding capacity (caffeine/benzene).8.....8. Hydrosphere C8 CAPCELL PAK C8 AQ Gemini C8 Pro C8 Develosil C-UG Atlantis T Unison UK-C8 Inertsil ODS- Hypersil GOLD CAPCELL PAK C8 MGII Gemini NX-C8 SunFire XTerra MS C8 XBridge C8 Inertsil ODS- Eternity-C8 Mightysil RP-8 C8 L-column ODS TSKgel ODS-S CAPCELL PAK C8 MGIII Cadenza CD-C8 L-column ODS Eluent : MeOH/W (8/) [amylbenzene], MeOH/W (/7) [caffeine, benzene].... 8.... (S98A) Hydrophobicity k (amylbenzene) Pro C8 RS Develosil ODS-SR 9 第 章.indd 9..8 :: AM
High performance reversed-phase column Features of Pro series ODS columns Comparison of separation behavior on the three kinds of ODS columns in the Pro series Parabenzoates types of ODS are available to separate the para-benzoates shown in the left, but the best separation between peaks and is achieved on Pro C8 RS.. OH COOCH Methyl p-hydroxybenzoate. OH COOCH CH Ethyl p-hydroxybenzoate. OH CH COOCH Reversed-Phase C8 Columns(. CH Isopropyl p-hydroxybenzoate OH COO(CH ) CH Propyl p-hydroxybenzoate min min min. OH CH COOCH CH CH Isobutyl p-hydroxybenzoate Column :. mmi.d. Eluent :methanol/water (/) Flow rate :. ml/min Temperature : Detection :UV at 7 nm. OH COO(CH ) CH Butyl p-hydroxybenzoate Flavonoid glycosides HO OH. Rutin. Isoquercitrin. Quercitrin. Quercetin O O OR HO R = rutinose R = glucose R = rhamnose R = H HO Hydrogen-bonding interactions significantly contribute to the retention of hydrophilic compounds, including glycosides. Therefore, the retention is longer in the Hydrosphere C8, which has strong hydrogen-bonding capacity, than in Pro C8 RS, which has highly hydrophobic packing materials. To separate such hydrophilic compounds, the Hydrosphere C8 is suitable for precise separation of multicomponent samples, while Pro C8 RS and Pro C8 are suitable for rapid separation of simple components. min Column :. mmi.d. Eluent :acetonitrile/ mm HPO (/7) Flow rate :. ml/min Temperature : Detection :UV at nm 第 章.indd..8 ::9 AM
Terphenyl isomers, Since Pro C8 RS has excellent hydrophobic selectivity and shape selectivity, it works well in the separation of structural isomers that are not easily separated by other ODS. Reversed-Phase C8 Columns(.. o-terphenyl m-terphenyl. min min min min p-terphenyl Column :. mmi.d. Eluent :methanol/water (8/) Flow rate :. ml/min Temperature :7 Detection :UV at nm Saikosaponins Peaks and, which are not easily separated by other ODS, can be completely separated by Hydrosphere C8. It is thought that hydrogen-bonding interaction of hydrophilic functional groups in sugar chain and terpene skeleton as well as hydrophobic interaction has great contribution to the separation of saikosaponins. Hydrosphere C8 is the most appropriate in the separation of this kind of class of compound.. O., H O glucose glucose rhamnose Saikosaponin c OH CHOH OH O CHOH fucose glucose Saikosaponin b,. O H OH O CHOH fucose glucose Saikosaponin a. O H OH O CHOH fucose glucose Saikosaponin d min Column :. mmi.d. Eluent :acetonitrile/water (8/) Flow rate :. ml/min Temperature :7 Detection :UV at nm 第 章.indd..8 :: AM
Reversed-Phase C8 Columns( Analytical columns YMC-Pack Pro C8 Superior separation of basic compounds Processed with advanced endcapping technology Excellent reproducibility Utilizes highly pure silica gel base Two types of test reports are included with each column Pore size: Å Carbon content:% Usable ph range:. 8. P e r f e c t l y e n d c a p p e d O D S YMC-Pack Pro C8 includes more advanced endcapping technology for strictly controlled processing of residual silanol groups that are likely to affect quality. Pro C8 is highly appropriate for basic compounds, including pharmaceutical products and agricultural chemicals. Two test reports are included with each product; a Column Test Report, indicating the column performance, and a Packing Material Test Report, detailing strictly controlled physical properties and separation characteristics of the packing material. Useful for separation of basic compounds Tricyclic antidepressants. HCI N CH CH CH NHCH Desipramine hydrochloride.. Acenaphthene(I.S.). Advanced endcapping technology results in good peak shapes even for basic compounds that often elute with poor tailing shapes on competitive columns. min HCI N CH CH CH CH N CH Imipramine hydrochloride HCI CHCH CH N CH CH Amitriptyline hydrochloride Column :YMC-Pack Pro C8 ( µm, Å). mmi.d. Eluent : mm KHPO-KHPO (ph.9)/ methanol (/7) Flow rate :. ml/min Temperature :7 Detection :UV at nm Basic peptides. Bradykinin. Dynorphin A (Human,-). Eledoisin related peptide. Neurotensin. Bombesin. Substance P YMC-Pack Pro C8 is processed using advanced endcapping technology and produces a better chromatogram than that obtained using ODS-A for separating basic peptides. Similar chromatograms have been obtained with YMC- Pack Pro C8 and ODS-A for separation of neutral peptides. min Neutral peptides min Column :YMC-Pack Pro C8 and ODS-A ( µm, Å) 7. mmi.d. Eluent :A) water/tfa (/.) B) acetonitrile/tfa (/.) -% B (- min,linear) Flow rate :. ml/min Temperature :7 Detection :UV at nm min min. Oxytocin. Met-Enkephalin. Leu-Enkephalin. Angiotensin l. α-mating factor. Insulin 第 章.indd..8 ::8 AM
Excellent lot-to-lot reproducibility batch-to-batch. Uracil. -n-butoxybenzoic acid. Ethylbenzene. n-propylbenzene. Caffeine. Pyridine. Phenol A three-lot comparison is shown on the left. Excellent reproducibility is achieved in separating hydrophilic, basic and acidic compounds, as well as hydrophobic compounds. Lot C Lot C Lot B Lot B Lot A Lot A Reversed-Phase C8 Columns( min 8 min column-to-column 8 9 8 Advanced packing technology results in high column efficiency. All packed columns are inspected and controlled according to the strictest quality control criteria for column performance ever, including theoretical plate number testing. Two types of test reports Packing Material Test Report Column Test Report Each YMC-Pack Pro C8 includes both, a Column Test Report and a Packing Material Test Report, to certify lotto-lot and column-to-column reproducibility. This report describes basic physical properties of silica gel, including particle size, pore size and metal content, carbon loading after modification, and the specifications and results of separation characteristics tests. The Column Test Report shows the theoretical plate number and asymmetry factor to indicate column performance. For convenience in deterioration evaluation, this report includes all analytical conditions including sample concentration as well. 第 章.indd..8 :: AM
Analytical columns YMC-Pack Pro C8 A p p l i c a t i o n, min min min Reversed-Phase C8 Columns( CHCOOH CHCOOH Maleic acid CH Methyl p-hydroxybenzoate NHCCHN(CHCH) CH Lidocaine OH COOCH O CH CH C CH CH H N N CH Naphazoline CH C CH Benzethonium chloride Cl CH + (OCHCH) N CH Cl - CH CHCHCHN CH CH Chlorpheniramine N Column :. mmi.d. Eluent :A) mm KHPO-HPO (ph.) B) methanol -9% B (- min), 9% B (- min) Flow rate :. ml/min Temperature :7 Detection :UV at nm A p p l i c a t i o n (NA) catechins. (-)-Epigallocatechin. (+)-Catechin. (-)-Epicatechin. (-)-Epigallocatechin gallate. (-)-Epicatechin gallate Column : YMC-Pack Pro C8 ( µm, Å). mmi.d. Eluent : acetonitrile/ethyl acetate/water/tfa (9//88/.) Flow rate :. ml/min Temperature : 7 Detection : UV at 8 nm min A p p l i c a t i o n (A97C) 7 antioxidants. n-propyl gallate(pg).,,-trihydroxybutyrophenone (THBP). tert-butylhydroquinone(tbhq). Nordihydroguaiaretic acid (NDGA). -Hydroxymethyl-,-di-tert-butylphenol (HMBP). n-octyl gallate(og) 7. n-dodecyl gallate(dg) Column :YMC-Pack Pro C8 ( µm, Å) 7. mm I.D. Eluent :A) acetonitrile/methanol/% acetic acid (//) B) acetonitrile/methanol/% acetic acid (//) -% B (- min), % B (- min) Flow rate :. ml/min Temperature : Detection :UV at 8 nm min 第 章.indd..8 :: AM
Ordering information Analytical columns Pore size (Å) S-. ASS-HWT. ASS-WT. ASS-WT. ASS-HPWT. ASS-PWT. ASS-PWT. ASS-HWT. ASS-WT. 7 ASS-LWT. ASS-WT. ASS-WT. ASS-WT. ASS-WT. ASS-WT. ASS-HWT. ASS-WT. 7 ASS-LWT. ASS-WT. ASS-WT. ASS-WT. 7 ASS-LWT. ASS-WT S-. ASS-WT. ASS-WT. ASS-PWT. ASS-PWT. ASS-HWT. ASS-WT. 7 ASS-LWT. ASS-WT. ASS-WT. ASS-WT. ASS-WT. 7 ASS-LWT. ASS-WT. ASS-WT. ASS-WT. ASS-WT. 7 ASS-LWT. ASS-WT. ASS-WT. ASS-WT. ASS-WT. ASS-WT S-. ASS-WT. ASS-WT Semi-preparative columns Pore size (Å) S- ASS-WT ASS-WT ASS-WT ASS-WT ASS-WT S- ASS-WT ASS-WT ASS-WT See P for preparative columns other than those listed above. Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) S-. ASS-CC. ASS-PCC. ASS-CC. ASS-GCC S-. ASS-CC. ASS-PCC. ASS-CC. ASS-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) XPCHSMW XPCHW YMC guard cartridge pack S-. ASS-GCCPK S-. ASS-GCCPK A guard cartridge pack includes a cartridge holder ( set), a guard cartridge column, and a column coupler (produced by PEEK). Guard columns S- ASS-WTG ASS-WTG S- ASS-WTG Reversed-Phase C8 Columns( When columns packed with material from different lots are needed for validation purposes, please contact us. 第 章.indd..8 :: AM
Reversed-Phase C8 Columns( Analytical columns Hydrosphere C8 Strong retention of hydrophilic compounds Can be used with % water mobile phase Superior separation of basic compounds Excellent reproducibility H y d r o p h i l i c O D S Utilizes highly pure silica gel base Two types of test reports are included with each column Pore size: Å Carbon content:% Usable ph range:. 8. Hydrosphere C8 is designed to maintain adequate hydrophilicity on the packing surface for superior separation of hydrophilic compounds. Hydrophilic compounds are retained with much greater affinity than with conventional ODS columns, permitting the product to be used with % water eluent, a problematic eluent for conventional ODS columns. Hydrosphere C8 is useful for separating a wide range of compounds, including nucleic acids and their derivatives, organic acids, saccharides, glycosides and peptides. Appropriate for separation of hydrophilic compounds Separation of organic acids, High hydrophilicity of the Hydrosphere C8 packing material surface enables longer retention and superior separation of citric acid and fumaric acid as compared to a conventional ODS column (Brand Ⅰ). min min. Lactic acid. Acetic acid. Citric acid. Fumaric acid. Succinic acid Column :. mm I.D. Eluent : mm NaHPO-HPO (ph.8) Flow rate :. ml/min Temperature : Detection :UV at nm Reproducibility of retention time when used with % water mobile phase After standing Before standing. Cytosine. Uracil. Guanine. Thymine. Adenine min 98 min min 89 min Column :. mm I.D. Eluent : mm KHPO-KHPO (ph.9) Flow rate :. ml/min Temperature :7 Detection :UV at nm min 7 min After completion of analysis, the flow was stopped and the columns were left to stand overnight (ca. hours). When conventional ODS columns are used with % water mobile phase, the apparent hydrophobicity decreases due to repulsive interactions between water and hydrophobic groups on the surface of the packing materials, reducing retention of compounds. In the experiment illustrated here, columns filled with % water mobile phase were left to stand overnight. Retention times of nucleic acid bases were analyzed before and after standing and compared. The retention times of adenine after standing decreased to 89% of the original value for Brand II and to 7% of the original Brand III value. By contrast, Hydrosphere C8 retained 98% of its original retention for adenine. Hydrosphere C8 is designed to maintain adequate hydrophilicity on the packing surface so that hydration can be achieved, and when Hydrosphere C8 is used with mobile phase containing no organic solvent, the retention time is not significantly shortened and highly reproducible chromatograms can be obtained. 第 章.indd..8 :: AM
Differences in separation characteristics from conventional ODS columns min min The chromatograms shown were obtained in tests conducted to compare separation characteristics using a mixed sample containing compounds with different characteristics. The packing material surface of Hydrosphere C8 has higher hydrophilicity and lower C8 ligand density than those of Pro C8. This results in shorter retention of hydrophobic compounds, including n-propylbenzene and n-butylbenzene, and planar compounds like acenaphthene. By contrast, retention of testosterone, a compound with large hydrogen-bonding properties, is longer on Hydrosphere C8 and on Brand IV, which also has a highly hydrophilic packing surface. Amitriptyline, a basic compound, caused tailing peaks in Brand IV and Brand V. By contrast, Hydrosphere C8 and Pro C8 produce good peak shapes for such basic compounds. Although the retention times of amitriptyline on Hydrosphere C8 and Pro 8 are similar, there is a big difference between the two columns with regard to resolution of n-butylbenzene and amitriptyline. This difference is considered to be due to the hydrophilicity of the Hydrosphere C8 surface. These results demonstrate that Hydrosphere C8, having different separation characteristics from conventional ODS columns, enables separation of compounds that cannot be separated using other ODS columns. Reversed-Phase C8 Columns( min 7 8 9 min. Toluene. Testosterone. n-propylbenzene. Acenaphthene. n-butylbenzene. Amitriptyline hydrochloride Column :. mm I.D. Eluent : mm KHPO-KHPO (ph.9)/methanol (/) Flow rate :. ml/min Temperature :7 Detection :UV at nm 7 第 章.indd 7..8 :: AM
Analytical columns Hydrosphere C8 Short analysis time Hydrosphere C8 can reduce the time necessary for analyzing compounds with high hydrophobicity by about % as compared with conventional ODS columns. Antioxidants. Nordihydroguaiaretic acid (NDGA). Butyl hydroxyanisol (BHA). -Hydroxy-,-di-tert-butylphenol (HMBP). n-octyl gallate (OG). Dibutyl hydroxytoluene (BHT). n-dodesyl gallate (DG) min min Column :. mm I.D. ( µm, Å) Eluent :acetonitrile/methanol/% acetic acid (//) Flow rate :. ml/min Temperature : Detection :UV at 8 nm Reversed-Phase C8 Columns( A p p l i c a t i o n Nicotinic acid and derivatives (F77B). Nicotinamide adenine dinucleotide phosphate oxidized form (NADP). Nicotinic acid. Nicotinamide adenine dinucleotide phosphate reduced form (NADPH). Nicotinamide. β-nicotinamide adenine dinucleotide (NAD). β-nicotinamide adenine dinucleotide reduced form (NADH) Column :Hydrosphere C8 ( µm, Å). mm I.D. Eluent : mm KHPO-KHPO (ph.9)/acetonitrile (99/) Flow rate :. ml/min Temperature :7 Detection :UV at nm min A p p l i c a t i o n (A9A) 7 8 9 Nucleotides. -UTP 7. -ATP. -UDP 8. -IMP. -CMP 9. -ADP. -UMP. -TMP. -dcmp. -AMP. -GMP Column :Hydrosphere C8 ( µm, Å). mm I.D. Eluent : mm KHPO-KHPO (ph.) Flow rate :. ml/min Temperature : Detection :UV at nm min 8 第 章.indd 8..8 ::7 AM
Ordering information Analytical columns Pore size (Å) S-. HSS-HWT. HSS-WT. HSS-WT. HSS-HPWT. HSS-PWT. HSS-PWT. HSS-HWT. HSS-WT. 7 HSS-LWT. HSS-WT. HSS-WT. HSS-WT. HSS-WT. HSS-WT. HSS-HWT. HSS-WT. 7 HSS-LWT. HSS-WT. HSS-WT. HSS-WT. 7 HSS-LWT. HSS-WT S-. HSS-WT. HSS-WT. HSS-PWT. HSS-PWT. HSS-HWT. HSS-WT. 7 HSS-LWT. HSS-WT. HSS-WT. HSS-WT. HSS-WT. 7 HSS-LWT. HSS-WT. HSS-WT. HSS-WT. HSS-WT. 7 HSS-LWT. HSS-WT. HSS-WT. HSS-WT. HSS-WT. HSS-WT Semi-preparative columns Pore size (Å) S- HSS-WT HSS-WT HSS-WT HSS-WT HSS-WT Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) S-. HSS-CC. HSS-PCC. HSS-CC. HSS-GCC S-. HSS-CC. HSS-PCC. HSS-CC. HSS-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) XPCHSMW XPCHW YMC guard cartridge pack S-. HSS-GCCPK S-. HSS-GCCPK A guard cartridge pack includes a cartridge holder ( set), a guard cartridge column, and a column coupler (produced by PEEK). Guard columns S- HSS-WTG HSS-WTG Reversed-Phase C8 Columns( 9 第 章.indd 9..8 ::8 AM
Analytical columns YMC-Pack Pro C8 RS Excellent acid resistance and alkali resistance (ph to ) Superior separation of structural isomers Superior separation of basic compounds H i g h l y d u r a b l e O D S Excellent reproducibility Utilizes highly pure silica gel base Two types of test reports are included with each column Pore size:8 Å Carbon content:% Usable ph range:.. YMC-Pack Pro C8 RS is a polymeric type high carbon ODS column characterized by high resolution and high durability. It is applicable to a wide range of compounds, providing good separation of basic compounds that easily cause tailing peaks. The separation selectivity for compounds that differ only slightly in hydrophobicity is outstanding. It also has excellent resistance to acid and alkali, making it useful under demanding separation conditions. Excellent durability Reversed-Phase C8 Columns( 8 YMC-Pack Pro C8 RS Acid-resistant column (manufactured by other company) Monomeric ODS 8 8 Pro C8 RS Monomeric ODS 8 A wide choice of mobile phases facilitates method development,, min min min min. Flurbiprofen. Methyl benzoate. Lidocaine. Diltiazem. Verapamil Column :YMC-Pack Pro C8 RS Eluent : mm acetate buffer/methanol (/) Flow rate :. ml/min Temperature : Detection :UV at nm Pro C8 RS with excellent acid and alkali resistance can be used over a wide ph range, and it is easy to optimize the separating conditions. In addition, by downsizing from the column (particle µm, length mm) to a shorter column (particle µm, length mm), with high separation ability, it becomes possible to shorten the analysis time by one third without affecting the separation. 第 章.indd..8 :: AM
Separation of basic compounds Tf(). Tf().87 Amitriptyline is a basic compound that can easily tail on some silica based columns. In comparison with competing acid-resistant columns, Pro C8 RS produces good peak shapes without tailing.. Desipramine. Diphenyl (I.S.). Imipramine. Amitriptyline min min Column :. mmi.d. Eluent : mm KHPO-KHPO (ph.9) /methanol (/7) Flow rate :. ml/min Temperature :7 Detection :UV at nm Separation of structural isomers Reversed-Phase C8 Columns( Pro C8 RS with a high C8 density is excellent in hydrophobicity recognition ability and plane recognition ability, and can be applied to separate structural isomers which are difficult to separate with other ODS. In addition, by using µm column packing, resolution can be increased, and it becomes possible to achieve a baseline separation of linolenate isomers. Rs=.8 Rs=. min min Methyl linolenate isomers. Methyl linolenate. Methyl γ-linolenate Column :. mmi.d. Eluent :acetonitrile/thf/water (//) Flow rate :. ml/min Temperature : Detection :UV at nm Rs=. min 第 章.indd..8 :: AM
Analytical columns YMC-Pack Pro C8 RS Separation of peptides by LC/MS.9 M+ Bradykinin(MW: 9.) Pro C8 RS produces good peak shapes when it is used with mobile phase containing low TFA concentration, making it suitable for LC/ MS..7 M+.7 8 (m/z) Reversed-Phase C8 Columns( 8....Time. [Arg 8 ]-Vasopressin. Bradykinin. Angiotensin Ⅱ. Oxytocin. Angiotensin Ⅰ. Leu-Enkephalin Column :YMC-Pack Pro C8 RS ( µm, 8 Å). mmi.d. Eluent :A) water/tfa (/.) B) acetonitrile/tfa (/.) -% B (- min) Flow rate :. ml/min Temperature :7 Detection :ESI positive-mode A p p l i c a t i o n Chloroacetophenone isomers Chlorophenol isomers. COCH Cl. OH Cl o-chloroacetophenone. COCH o-chlorophenol. OH Cl p-chloroacetophenone Cl p-chlorophenol. COCH. OH Cl m-chloroacetophenone Cl m-chlorophenol min Column :YMC-Pack Pro C8 RS ( µm, 8 Å). mmi.d. Eluent :acetonitrile/water (/) Flow rate :. ml/min Temperature : Detection :UV at nm min Column :YMC-Pack Pro C8 RS ( µm, 8 Å). mmi.d. Eluent : acetonitrile/water/acetic acid (/7/) Flow rate :. ml/min Temperature :7 Detection :UV at nm 第 章.indd..8 ::9 AM
Ordering information Analytical columns Pore size (Å) S- 8. RS8S-HWT. RS8S-WT. RS8S-WT. RS8S-HPWT. RS8S-PWT. RS8S-PWT. RS8S-HWT. RS8S-WT. 7 RS8S-LWT. RS8S-WT. RS8S-WT. RS8S-WT. RS8S-WT. RS8S-WT. RS8S-HWT. RS8S-WT. 7 RS8S-LWT. RS8S-WT. RS8S-WT. RS8S-WT. 7 RS8S-LWT. RS8S-WT S- 8. RS8S-WT. RS8S-WT. RS8S-PWT. RS8S-PWT. RS8S-HWT. RS8S-WT. 7 RS8S-LWT. RS8S-WT. RS8S-WT. RS8S-WT. RS8S-WT. 7 RS8S-LWT. RS8S-WT. RS8S-WT. RS8S-WT. RS8S-WT. 7 RS8S-LWT. RS8S-WT. RS8S-WT. RS8S-WT. RS8S-WT. RS8S-WT Semi-preparative columns Pore size (Å) S- 8 RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-WT Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) S- 8. RS8S-CC. RS8S-PCC. RS8S-CC. RS8S-GCC S- 8. RS8S-CC. RS8S-PCC. RS8S-CC. RS8S-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) XPCHSMW XPCHW YMC guard cartridge pack S- 8. RS8S-GCCPK S- 8. RS8S-GCCPK A guard cartridge pack includes a cartridge holder ( set), a guard cartridge column, and a column coupler (produced by PEEK). Guard columns S- 8 RS8S-WTG RS8S-WTG Reversed-Phase C8 Columns( 第 章.indd..8 :: AM
Reversed-Phase C8 Columns( Analytical columns YMC-Pack ODS-A Conventional ODS column Currently in use worldwide Pore size: Å, Å, Å Carbon content:7% ( Å), % ( Å), 7% ( Å) Usable ph range:. 7. S t a n d a r d O D S YMC-Pack ODS-A has a highly endcapped surface structure and appropriate hydrophobicity for separation of a wide range of compounds. It is produced under strict quality control with respect to or more parameters in order to ensure stable quality. This product is highly regarded as the standard YMC- Pack packing material in various countries around the world. Quality control system for excellent performance tr (Butyl benzoate), min 8 The graph indicates lot-to-lot reproducibility with respect to hydrophobic interaction for lots, an essential column performance characteristic. Extremely stable lot-to-lot reproducibility of the hydrophobic surface is achieved by strict control of variables. In addition to measurement of the physical properties, a wide range of compounds including hydrophobic, acidic, basic and coordination compounds are analyzed under optimal conditions to evaluate column performance. 7 8 9 Lot Number A p p l i c a t i o n (S9D) A p p l i c a t i o n (G9Q) Analgesics CH COHN OH Acetaminophen CONH OCH Ethenzamide HC O N O N CH Caffeine CH =CHCH HC HC CH N N CHCHCONHCONH Apronalide Carotenes.α-Carotene.β-Carotene min Column :YMC-Pack ODS-A ( µm, Å). mmi.d. Eluent :methanol/water (/) Flow rate :.7 ml/min Temperature : Detection :UV at nm min Column :YMC-Pack ODS-A ( µm, Å). mmi.d. Eluent :acetonitrile/methanol/thf (//) Flow rate :. ml/min Temperature : Detection :UV at nm Ordering information Analytical columns Pore size (Å) S-. AAS-HWT. AAS-WT. 7 AAS-LWT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT Pore size (Å) S-. 7 AAS-LWT. AAS-WT. AAS-WT. AAS-WT. AAS-WT 第 章.indd..8 :: AM
Ordering information Analytical columns Pore size (Å) S-. AAS-WT. AAS-WT. AAS-PWT. AAS-PWT. 7 AAS-LWT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. 7 AAS-LWT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-PWT. AAS-PWT. 7 AAS-LWT. AAS-WT. AAS-WT. 7 AAS-LWT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT S-. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT. AAS-WT Semi-preparative columns Pore size (Å) S- AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT Semi-preparative columns Pore size (Å) S- AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT See P for preparative columns other than those listed above. Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) S-. AAS-CC. AAS-GCC S-. AAS-CC. AAS-PCC. AAS-CC. AAS-GCC. AAS-GCC. AAS-CC. AAS-PCC. AAS-CC. AAS-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) YMC guard cartridge pack XPCHSMW XPCHW S-. AAS-GCCPK S-. AAS-GCCPK A guard cartridge pack includes a cartridge holder ( set), a guard cartridge column, and a column coupler (produced by PEEK). Guard columns S-. AAS-WFG. AAS-WFG S-. AAS-WFG. AAS-WFG AAS-WTG AAS-WTG. AAS-WFG AAS-WTG. AAS-WFG. AAS-WFG AAS-WTG AAS-WTG S-. AAS-WFG. AAS-WFG AAS-WTG AAS-WTG. AAS-WFG. AAS-WFG AAS-WTG. AAS-WFG. AAS-WFG AAS-WTG AAS-WTG Reversed-Phase C8 Columns( 第 章.indd..8 :: AM
Analytical columns YMC-Pack ODS-AM Similar to ODS-A in selectivity Excellent reproducibility Useful for quality control purposes Pore size: Å Carbon content:7% Usable ph range:. 7. O D S w i t h o u t s t a n d i n g l o t - t o - l o t r e p r o d u c i b i l i t y YMC-Pack ODS-AM is a product which especially emphasizes lot-to-lot reproducibility of the packing material. Strict control is performed on all aspects of quality, including physical properties of silica gel base and surface modifying processes, in order to ensure stable quality. Double endcapping is conducted to ensure that lot-to-lot reproducibility is maintained. Special attention is given to lot-to-lot reproducibility Reversed-Phase C8 Columns( (Methylparben/,-Dimethylpyridine) 8 The separation factor (α) of methylparaben/,- dimethylpyridine for lots of ODS-AM packing material is plotted on the left graph. Excellent lot-to-lot reproducibility is achieved even in separation of basic compounds. 7 8 9 Lot Number Acidic compounds Coordination compounds. Uracil. Benzoic acid. p-toluic acid. p-ethylbenzoic acid. Methy benzoate. Uracil. Acetylacetone. 8-Quinolinol Secondary interaction of the stationary phase surface may affect peak shapes of acidic compounds. ODS-AM can provide chromatograms without tailing peaks as shown above. The peak shapes of coordination compounds, including 8-quinolinol and acetylacetone, may be affected by the presence of metal impurities on the surface of the silica. The distribution level of metal impurities on the column is strictly controlled for ODS-AM, providing excellent peak symmetry. 第 章.indd..8 :: AM
A p p l i c a t i o n (G9J) Alkaloids 7 8 9. Hordenine sulfate. Pilocarpine chloride. Scopolamine bromide. Atropine sulfate. Cinchonine. Sparteine sulfate 7. Quinine 8. Papaverine chloride 9. Narcotine chloride Reversed-Phase C8 Columns( 8 min Column :YMC-Pack ODS-AM ( µm, Å). mmi.d. Eluent :acetonitrile/ mm KHPO (/8) Flow rate :. ml/min Temperature : Detection :UV at nm Ordering information Analytical columns Pore size (Å) S-. AMS-HWT. AMS-WT. 7 AMS-LWT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. 7 AMS-LWT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. AMS-WT S-. AMS-WT. AMS-WT. AMS-PWT. AMS-PWT. 7 AMS-LWT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. 7 AMS-LWT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. AMS-WT. AMS-WT Semi-preparative columns S- AMS-WT AMS-WT AMS-WT AMS-WT AMS-WT AMS-WT See P7 for preparative columns other than those listed above. Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) Pore size (Å) S-. AMS-CC. AMS-GCC S-. AMS-CC. AMS-PCC. AMS-CC. AMS-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) YMC guard cartridge pack XPCHSMW XPCHW S-. AMS-GCCPK S-. AMS-GCCPK A guard cartridge pack includes a cartridge holder ( set), a guard cartridge column, and a column coupler (produced by PEEK). Guard columns S-. AMS-WFG. AMS-WFG S-. AMS-WFG. AMS-WFG AMS-WTG AMS-WTG 7 第 章.indd 7..8 ::7 AM
Analytical columns YMC-Pack ODS-AQ Useful for separation of hydrophilic compounds Can be used with % water in mobile phase Different selectivity from conventional ODS Pore size: Å, Å Carbon content:%, % Usable ph range:. 7. H y d r o p h i l i c O D S YMC-Pack ODS-AQ has moderate hydrophobicity and hydrogen-bonding capacity. It shows different retention behavior from that of YMC-Pack ODS-A, for samples with relatively high hydrophilicity. It is useful in fields including carbohydrate chemistry for oligosaccharides and glycosides, pharmacognosy and natural product chemistry. Useful for separation of sugars This product is useful for separations using mobile phases in which water content is relatively high, such as separation of sugars and glycosides. Reversed-Phase C8 Columns( G G G G G. Maltoundecaose(G). Maltododecaose(G). Maltotridecaose(G). Maltotetradecaose(G). Maltopentadecaose(G) min Column :YMC-Pack ODS-AQ ( µm, Å). mmi.d. Eluent :methanol/water (/99) Flow rate :. ml/min Temperature :7 Detection :RI, 8 - RIU/FS (M9M) Hydrogen-bonding capacity Chromatograms of purine alkaloids are shown on the left. As compared with ODS-A, ODS-AQ and ODS-AL show longer retention of caffeine and theophylline relative to phenol, an internal standard.. Trigonelline. Theobromine. Theophylline. Caffeine. Phenol (Phenol/Caffeine).8... Change of separation factor (α) of caffeine/phenol according to temperature change in ODS-AQ, ODS-A and ODS-AL is plotted on the graph shown on the left. Although caffeine is considered to have stronger hydrogenbonding properties than phenol, the hydrogen-bonding capacity of stationary phase may affect the α of caffeine/ phenol. The smaller the hydrogen-bonding capacities are, the higher α of phenol/caffeine. ODS-AQ shows different retention behavior from that of ODS-A since it has moderate hydrogen-bonding capacity....... ( ) - / T,K ( ) 8 第 章.indd 8..8 :: AM
A p p l i c a t i o n (G97E) 7 8 Crude drugs (glycosides). Arbutin. Salicin. Esculine. G-Strophantine(Ouabain). Baicalin. Barbaloin 7. Glycyrrhizin 8. Digitoxine min Column :YMC-Pack ODS-AQ ( µm, Å). mmi.d. Eluent :A) methanol/ mm NHHPO (/9) B) methanol/ mm NHHPO (8/) -% B (- min,linear),% B (- min) Flow rate :. ml/min Temperature : Detection :UV at nm Ordering information Analytical columns Pore size (Å) S-. AQS-HWT. AQS-WT. 7 AQS-LWT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT S-. AQS-WT. AQS-WT. AQS-PWT. AQS-PWT. 7 AQS-LWT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. 7 AQS-LWT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT. AQS-WT Semi-preparative columns S- AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT S- AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT See P for preparative columns other than those listed above. Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) Pore size (Å) S-. AQS-CC. AQS-GCC S-. AQS-CC. AQS-PCC. AQS-CC. AQS-GCC. AQS-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) YMC guard cartridge pack XPCHSMW XPCHW S-. AQS-GCCPK S-. AQS-GCCPK A guard cartridge pack includes a cartridge holder ( set), a guard cartridge column, and a column coupler (produced by PEEK). Guard columns S-. AQS-WFG. AQS-WFG S-. AQS-WFG. AQS-WFG AQS-WTG AQS-WTG. AQS-WFG AQS-WTG S- AQS-WTG AQS-WTG AQS-WTG Reversed-Phase C8 Columns( 9 第 章.indd 9..8 :: AM
Analytical columns YMC-Pack ODS-AL ODS with residual silanol groups Utilizes secondary interaction caused by silanol groups Pore size: Å Carbon content:7% Usable ph range:. 7. N o n - e n d c a p p e d O D S YMC-Pack ODS-AL uses not only hydrophobic interaction but also secondary interaction caused by silanol groups that affect separation. This results in a different selectivity from conventional ODS columns. When ionic interaction is utilized, it is preferable to use a buffer in the mobile phase to achieve reproducibility of chromatograms. Effects of silanol group The separation factor (α) of internal standards methylparaben/,-dimethylpyridine for ODS-AM is different from that of ODS-AL, which is not endcapped. ODS-AL, which is affected by residual silanol, shows relatively great retention of pyridines. Reversed-Phase C8 Columns( (/)=. (/)=.8. Uracil. Pyridine.,-Dimethylpyridine. Methylparaben Utilizes residual silanol groups for separation, The figure shows separation of disinfectants under the conditions described in the Japanese Pharmacopeia. The object is to select the column permitting the elution of benzoic acid, salicylic acid and theophylline, in this order, insuring complete separation of these peaks. With ODS-AQ, separation of salicylic acid and theophylline is incomplete. By contrast, ODS-AL provides excellent separation. Thus, ODS-AL may provide excellent separation when the separation conditions cannot be optimized on other ODS columns. Disinfectants. COOH. COOH OH Benzoic acid Salicylic acid. O HC N O N H N N CH Theophylline (I.S.). Phenol OH min min Column :YMC-Pack ODS-AL and ODS-AQ ( µm, Å). mmi.d. Eluent : mm KHPO-NaHPO (ph 7.)/methanol (7/) Flow rate :.8 ml/min Temperature : Detection :UV at 7 nm (Conditions described in Japanese Pharmacopoeia th ed.) 第 章.indd..8 :: AM
A p p l i c a t i o n Phenacyl esters of bile acids from pig gall. Cholic acid (CA). Hyodeoxycholic acid (HDCA). Chenodeoxycholic acid (CDAC) (G97B) Reversed-Phase C8 Columns( min Column : YMC-Pack ODS-AL ( µm, Å). mmi.d. Eluent :acetonitrile/water (/) Flow rate :. ml/min Temperature : Detection :UV at nm Ordering information Analytical columns Pore size (Å) S-. ALS-WT. ALS-WT. ALS-PWT. ALS-PWT. ALS-WT. ALS-WT. 7 ALS-LWT. ALS-WT. ALS-WT. ALS-WT. ALS-WT. ALS-WT. ALS-WT. ALS-WT. ALS-WT Semi-preparative columns S- ALS-WT ALS-WT ALS-WT ALS-WT ALS-WT ALS-WT See P7 for preparative columns other than those listed above. Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) Pore size (Å) S-. ALS-CC. ALS-PCC. ALS-CC. ALS-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) XPCHSMW XPCHW Guard columns S-. ALS-WFG. ALS-WFG ALS-WTG ALS-WTG 第 章.indd..8 :: AM
Reversed-Phase C8 Columns( Analytical columns J sphere ODS-H8, ODS-M8, ODS-L8 Useful for method development High theoretical plate number Pore size:8 Å Carbon content:ods-h8 %, ODS-M8 %, ODS-L8 9% Usable ph range:ods-h8. 9., ODS-M8 L8. 7. T h r e e t y p e s o f O D S, e a c h w i t h d i f f e r e n t l i g a n d c o v e r a g e J sphere offers a choice from three kinds of ODS made from the same silica gel base, each with different ligand coverage. Differences in ligand coverage considerably affect the hydrophobic retention behavior of solutes, as well as the separation behavior resulting from solute functional groups or tertiary structure. J sphere is useful for the optimization of separation conditions, since there is almost no need to consider interactions other than hydrophobic and hydrogen-bonding interactions (e.g., ionic or coordinate interaction). Three types of ODS ODS-H8 ODS-M8 ODS-L8 C nh n+(n=-8) Alkylbenzenes min Column :J sphere. mmi.d. Eluent :acetonitrile/water (7/) Flow rate :. ml/min Temperature :7 Detection :UV at nm Differences in separation characteristics J sphere ODS-H8 BRAND-B. BRAND-F.9 YMC-Pack ODS-AM. BRAND-G. J sphere ODS-M8. BRAND-A. BRAND-C.. J sphere ODS-L8 k (octylbenzene). J sphere ODS-H8 BRAND-F. BRAND-G. YMC-Pack ODS-AM. BRAND-C. BRAND-B. J sphere ODS-M8.7 BRAND-A J sphere ODS-L8.. (caffeine/phenol) 9.... ODS columns with different ligand coverage have different hydrophobicity, hydrogen bonding capacity and steric selectivity for tertiary structures. ODS-H8 has exceptionally high hydrophobicity and low hydrogen bonding capacity, ODS-M8 has moderate hydrophobicity and hydrogen bonding capacity and ODS-L8 has low hydrophobicity, comparable to that of C8 and high hydrogen bonding capacity. J sphere can be used with confidence to identify structural differences of solutes and is useful for improving the efficiency of separation optimization. 第 章.indd..8 ::9 AM
Ordering information Analytical columns Packing material Pore size (Å) ODS-H8 S- 8. JH8S-WT. JH8S-WT. JH8S-PWT. JH8S-PWT. 7 JH8S-LWT. JH8S-WT. JH8S-WT. JH8S-WT. JH8S-WT. JH8S-WT. 7 JH8S-LWT. JH8S-WT. JH8S-WT. JH8S-WT. JH8S-WT ODS-M8 S- 8. JM8S-WT. JM8S-WT. JM8S-PWT. JM8S-PWT. 7 JM8S-LWT. JM8S-WT. JM8S-WT. JM8S-WT. JM8S-WT. JM8S-WT. 7 JM8S-LWT. JM8S-WT. JM8S-WT. JM8S-WT. JM8S-WT ODS-L8 S- 8. JL8S-WT. JL8S-WT. JL8S-PWT. JL8S-PWT. JL8S-HWT. 7 JL8S-LWT. JL8S-WT. JL8S-WT. JL8S-WT. JL8S-WT. JL8S-WT. 7 JL8S-LWT. JL8S-WT. JL8S-WT. JL8S-WT. JL8S-WT Semi-preparative columns Packing material Pore size (Å) ODS-H8 S- 8 JH8S-WT JH8S-WT JH8S-WT JH8S-WT ODS-M8 S- 8 JM8S-WT JM8S-WT JM8S-WT JM8S-WT ODS-L8 S- 8 JL8S-WT JL8S-WT JL8S-WT JL8S-WT Guard cartridge columns (with inner diameter.,., or. mm: /pack,. mm: /pack) ODS-H8 S- 8. JH8S-CC. JH8S-PCC. JH8S-CC. JH8S-GCC ODS-M8 S- 8. JM8S-CC. JM8S-PCC. JM8S-CC. JM8S-GCC ODS-L8 S- 8. JL8S-CC. JL8S-PCC. JL8S-CC. JL8S-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) XPCHSMW XPCHW YMC guard cartridge pack ODS-H8 S- 8. JH8S-GCCPK ODS-M8 S- 8. JM8S-GCCPK ODS-L8 S- 8. JL8S-GCCPK A guard cartridge pack includes a cartridge holder ( set), a guard cartridge column, and a column coupler (produced by PEEK). Reversed-Phase C8 Columns( Guard columns ODS-H8 S- 8 JH8S-WTG JH8S-WTG ODS-M8 S- 8 JM8S-WTG JM8S-WTG ODS-L8 S- 8 JL8S-WTG JL8S-WTG 第 章.indd..8 :: AM
Reversed-Phase C8 Columns( Analytical columns YMC-Pack PolymerC8 Utilizes polymer base which is not affected by silanol Different separation characteristics from silica-based ODS Excellent ph stability Usable ph range:.. P o l y m e r t y p e C 8 PolymerC8 is a C8 column made from methacrylate polymer. It has excellent ph stability and it is useful for separation of basic compounds because there are no silanol or metal impurities to cause secondary interaction. Since π electrons of the carbonyl group or the hydroxyl group on the surface of the base material show particular interactions with solutes, PolymerC8 can show different separation characteristics from silica-based ODS. Can be used with alkali mobile phase. Barbital. Phenobarbital. Pentobarbital. Hexobarbital. Secobarbital min Column :YMC-Pack PolymerC8. mmi.d. Eluent : mm NaHPO-NaPO(pH.)/methanol (7/) Flow rate :. ml/min Temperature : Detection :UV at nm Excellent resistance to acid and alkali After hours min min min min 第 章.indd..8 :: AM
Different separation characteristics from silica-based ODS (aromatic compounds) Polyphenol. OH. OH. OH HO OH OH Phloroglucinol Resorcinol Phenol min min Column :YMC-Pack PolymerC8 and YMC-Pack ODS-A. mmi.d. Eluent :methanol/water (/) Flow rate :. ml/min Temperature : Detection :UV at 8 nm Different separation characteristics from silica-based ODS (unsaturated compounds), Triterpene saponins.. 8 COO--D-Glc HO HO 9 COO--D-Glc Reversed-Phase C8 Columns( HO HO CHOH CHOH. 7... min. 7... min Column :YMC-Pack PolymerC8 and YMC-Pack ODS-AM. mmi.d. Eluent :methanol/water (/) for PolymerC8 methanol/water (/) for ODS-AM Flow rate :.8 ml/min Temperature : Detection :UV at nm Ordering information Analytical columns S-. 7 PC99S-LWT. PC99S-WT. PC99S-WT. PC99S-WT. PC99S-WT. PC99S-WT S-. PC99S-WT. PC99S-WT. PC99S-WT. PC99S-WT Semi-preparative columns S- PC99S-WT S- PC99S-WT PC99S-WT Guard cartridge columns (with inner diameter. mm: /pack,. mm: /pack) S-. PC99S-CC. PC99S-GCC A guard cartridge holder will need to be purchased separately before using this product for the first time. Cartridge holder Semi-micro guard cartridge holder (inner diameter:.,., or. mm) Cartridge holder (inner diameter:. mm) XPCHSMW XPCHW Guard columns S-. PC99S-WFG. PC99S-WFG PC99S-WTG S-. PC99S-WFG. PC99S-WFG PC99S-WTG PC99S-WTG 第 章.indd..8 ::7 AM
第 章.indd..8 ::7 AM