Course Contents. MS Forensic Chemistry



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vii TABLE OF CONTENTS CHAPTER TITLE PAGE DECLARATION DEDICATION ACKNOWLEDGEMENT ABSTRACT ABSTRAK

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Course Contents MS Forensic Chemistry Year 1 Semester - I Course Code Course Title Credit Hours FCHEM-7105 Introduction to Forensic Science 3 FCHEM-7106 Crime Scene Investigation 3 FCHEM-7107 Chemistry of Drug of Abuse 3 FCHEM-7108 Analytical Techniques in Forensic Chemistry 3 Semester - II FCHEM-7204 Chemistry of Arsons, Explosives and Firearms 3 FCHEM-7205 DNA and Serology 3 FCHEM-7206 Forensic Law ( updated with 7105 and removed from syllabus since 2013) 3 FCHEM-7207 Statistics and Quality Control in Forensics 3 FCHEM-7208 Forensic Analysis Lab 3 FCHEM-7209 Nano Forensics 3 Year 2 Semester - III & IV FCHEM-7301 Journal Club 0 FCHEM-7302 Internship 1 FCHEM-7499 Thesis 12 1

First Semester Subject Title / Credit Hours: Introduction to Forensic Science Credit hrs. 3 Course Code: FCHEM-7105 Year: 2013 Semester: I Forensic Science: History and Scope of Forensic Science. Forensic Ethics, Forensic Investigation, Introduction to Forensic Toxicology, Forensic Radiology, Environmental Forensics. Fundamental Concepts in Biology (Cell, DNA, RNA etc.), Forensic Odontology, Forensic Anthropology, Forensic Entomology, Forensic Botany. Legal Aspects of Forensic Science: Forensic Science in Pakistan, Root Causes of Crime and Terrorism, Tiers of Criminal Justice System, Role of Forensics in Judicature, Investigation and Prosecution of Pakistan. Evidentiary Value of Physical and Biological Evidences and their Admissibility in Criminal and Civil Cases, Limitations of Forensic Analysis, Expert Opinion and Role of Forensic Expert, Post Conviction Remedies for Innocence Claims. Subject Title / Credit Hours: Crime Scene Investigation Credit hrs. 3 Course Code: FCHEM-7106 Semester: I A Brief History of Forensic Investigation, Protecting the Evidence: Specialized crime scene procedures, Utilizing correct weapons collection procedures, Collecting traffic crash evidence, Recovering buried bodies and surface skeletons, Applying specialized processing techniques, Interpreting a Crime Scene and Setting Crime Scene Perimeters Identifying Physical Evidence: Blood splatter studies, Firearms and Tool Mark, Fingerprinting analysis, Latent Fingerprint Development, Trace Evidence Examination Reconstruction of a Crime Scene Types of Evidence Preparing crime scene related documents: Documenting with personal notes, Writing reports in accepted police format, Diagramming crime scene using computers, Dictating reports, Generating reports using computer software. Other investigative personnel/agencies: Analyzing all known data to implement plan of action, Implementing the plan of action by coordinating with other investigative personnel/agencies. Receiving evidence for analysis in lab, documenting chain of custody, and evidence security. Dealing with Witnesses and Family Members Exercises: Actual cases, accompanied by crime scene photographs for a factual view of techniques, procedures and strategies utilized by crime investigators. 2

Subject Title / Credit Hours: Chemistry of Drugs of Abuse Credit hrs. 3 Course Code: FCHEM-7107 Semester: I Drug chemist and drug analysis, The work-flow of the drug analysis, classification/ Identification of drugs of abuse, Drug Classes and Controlled Substances Act, Drug Dependence and Addiction, Drugs as Evidence- The Five P s, The chemistry, origin and identification of important CNS Depressants, Alcohol, Opioids, Cannabinoids, Hallucinogens, CNS Stimulants, CNS Volatile Substances, Predator Drugs, Human Performance Drugs Subject Title / Credit Hours Analytical Techniques in Forensic Chemistry Credit hrs. 3 Course Code: FCHEM-7108 Year: 2013 Semester: I Separation Techniques Introduction, methods and interpretation of Paper Chromatography, Thin Layer Chromatography, Gas Chromatography, Liquid Chromatography, Ion Chromatography, Capillary Electrophoresis and their applications in drugs, toxicology, explosives, gunshot residues, fibres and documents. Elemental Analysis Introduction, methods and interpretation of Atomic Spectrometry, inductively coupled plasma mass spectrometry, X-ray Fluorescence spectroscopy and their applications in glass, gunshot residue, toxicology and paint. Molecular Spectroscopy Introduction, methods and interpretation of Infrared Spectroscopy, Raman Spectroscopy, UV-Visible Spectroscopy, Fluorescence Spectroscopy, Nuclear Magnetic Resonance Spectroscopy and their applications in paint, fibres, polymers, documents, explosives, drugs, Toxicology and body fluid. Mass Spectrometry Introduction, methods and interpretation of Molecular Mass Spectrometry, Ion Mobility Spectrometry and their applications in explosives and drugs. Thermal Analysis Introduction, methods and interpretation of Pyrolysis techniques, Differential Scanning Calorimetry, Differential Thermal Analysis, Thermogravimetric Analysis and their applications in polymers, explosives, arsons residues, fibres, paints and documents. 3

Second Semester Subject Title / Credit Hours Chemistry of Arsons, Explosives and Firearms Credit hrs. 3 Course Code: FCHEM-7204 The Chemistry of Combustion and Arsons: The Combustion continuum, Four aspects of Combustion, Combustion Deflagration and Detonation, Deflagration and Fires, Arson and fire investigation, Explosives: Explosives Power, Low and high Explosives and their uses, Field Screening Methods, Laboratory Methods Firearms and Propellants: Gun shot Residues, Elemental, Inorganic and Organic Analysis Subject Title / Credit Hours DNA and Serology Credit hrs. 3 Course Code: FCHEM-7205 DNA Fingerprinting: The basics of population genetics Allele frequency differences, Inbreeding coefficients, Most common genetic marker systems used in the forensic community: use of VNTR, SNP, and STR markers, DNA analysis methodologies, including the CODIS database, Techniques include extraction protocols, amplication of DNA, methods for labeling DNA, and ultimately construction of a genetic profile. Mitochondrial DNA, Parentage testing, Y-chromosome, and Amelogenin, Most commonly used gender identification locus, Dealing with mixtures and incomplete profiles. Serology: Screening Evidence for Biological Stains in Forensic Casework, Theory and methodology used in the examination and identification of body fluid stains, Determination of species origin and sources of false positive and negative, Collection and Storage of Biological Evidence, Chemical and Microscopic Analysis of Biological Stains including blood, semen and saliva, Structure and identification of hair, ABO Grouping and Secretor Status, Biological Markers of Forensic Significance. 4

Subject Title / Credit Hours Statistics and Quality Control in Forensics Credit hrs. 3 Course Code: FCHEM-7207 Year: 2012 Statistics in Forensics Probability of solving crime, to stop crime, to recover murder weapon and to catch criminal. Percentage of Drug addicts, Concentration of Drugs, to calculate the percentage of next drug users (chemometrics). Implementation of statistics in forensic analytical techniques. Evidence evaluation & evidence interpretation. DNA (data base searches and value of evidence). Sampling & sample size estimation of Evidence (Chemicals: explosive, drugs etc). Percentage of evidence correction (DNA, Fingerprint, IRIS recognition). Statistical data analysis of machine learning, pattern recognition, image analysis, information retrieval, and bioinformatics (Clustering) Quality Control in Forensics Introduction to QA/QC, Key Elements of a QA/QC programs in forensic science. Laboratory accreditation, staff accreditation, standard operating procedures, study protocol. Evidence Quality (Chain of Custody, Instrument reliability, Peer review on the evidence Analysis, Quality of results). Subject Title / Credit Hours Forensic Analysis Lab Credit hrs. 3 Course Code: FCHEM-7208 Physical Evidence Lab Procedures in Fingerprint analysis, latent fingerprint development etc. Microscopic analysis of trace evidence, Spot test and analysis of Arsons and Explosives Drug Analysis Lab Presumptive Tests, TLC, GCMS and HPLC of Drugs including Caffeine, Alcohol, Heroin, Morphine, Cocaine, Cannabinoids, Barbiturates, Benzodiazepines DNA Analysis Methods of extraction of DNA, DNA Amplification and STR Analysis etc. 5

Subject Title / Credit Hours Nano Forensics Credit hrs. 3 Course Code: FCHEM-7209 Year: 2012 Introduction to Nanotechnology: Nanoscale, Nanometer, Nanoparticles, Nanotubes, Thin films, Nanocomposites, Nanostructured bulk materials, Mesoporous, microporous and macroporous materials. Synthesis of nanomaterials with Bottom Up and Top Down approaches. Methods of chemical synthesis: anodization, hydrothermal, convention heating, deposition-precipiation methods and influence of reaction parameters. Characterization of nanomaterials by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Brunauer, Emmett and Teller (BET) adsorption method, Thermogravimetric Analysis (TGA), Atomic Absorption Spectroscopy (AAS), Ultraviolet-Visible Spectroscopy (UV-Vis) and Fourier Transform Infrared (FTIR) spectroscopy. Applications of nanomaterials: Destruction of chemical warfare agents, Decontamination and degradation of hazards materials, Latent fingerprint enhancement, Trace explosive, PCR efficiency, Trace evidence. Use of Micro X-ray Fluorescence (µxrf), Powder X-ray diffraction (XRD), Atomic force microscope (AFM) and Fourier Transform Infrared (FTIR) in Residue visualization, Questioned documents, Forensic pathology, Bloodstain and Traces. 6

Third and Fourth Semester Subject Title / Credit Hours Internship Credit hrs. 1 Course Code: FCHEM-7302 I & IV The students shall complete 4-week internship in an organization/agency related to forensic science. For this purpose the students shall be divided into several groups pertaining to different aspects of the forensic chemistry and shall work in a relevant organization/agency. This course carries a weight of one credit hour. After 4-week internship, a report shall be submitted in proper format. The manuscript shall be evaluated by the external and internal examiners. Subject Title / Credit Hours Journal Club Credit hrs. Nil Course Code: FCHEM-7301 I&IV Each student shall deliver a seminar on a publication in a reputed international journal of Forensic Science. For qualifying this course every student must attend at least 9 sessions. Each student shall submit a summary of each presentation including the title, name of the author/s and the journal, and get it signed by the teacher in-charge. 7