Proficiency testing schemes Helping you deliver analytical excellence

Transcription

1 Proficiency testing schemes Helping you deliver analytical excellence espoke and closed programmesfood and feed water and environment beverage clinical consumer safety forensics online reporting b LGC Quality - ISO/IEC ISO 9001

2 Contents 3 Introduction 37 Clinical PT schemes LGC Standards - your first choice in proficiency testing Aim of proficiency testing Quality standards Benefits of proficiency testing Who should participate in proficiency testing schemes? Who participates in LGC Standards proficiency testing schemes? Why choose LGC Standards as your proficiency testing provider? Typical scheme cycle from start to finish PORTAL - Proficiency Online Reporting and Trend AnaLysis Food and feed PT schemes Selector chart AFPS Animal feed QCS Chocolate QDCS Dairy chemistry QFCS Food chemistry QGS Gelatine QMAS Meat and fish QMS Food microbiology Water and environment PT schemes Selector chart AIR PT Air and stack emissions AQUACHECK Water, agricultural soils and sludges CONTEST Contaminated land QWAS Water microbiology Beverage PT schemes Selector chart BAPS Brewing analytes DAPS Alcoholic drinks MAPS Malt analytes QBS Soft drinks and fruit juices Selector chart DAH Drugs of abuse in hair DAU Drugs of abuse in urine DOF Drugs in oral fluid TDM Therapeutic drugs monitoring TOX Toxicology Consumer safety PT schemes Selector chart COSMETICS Cosmetics and toiletries NiMS Nickel migration PHARMASSURE Pharmaceutical TOYTEST Toy safety Forensics PT schemes Selector chart FAE Forensic analysis for explosives FIRMS Forensic isotope ratio mass spectrometry QUARTZ Forensic blood toxicology Other PT schemes Selector chart OIL PT Oils and fuels QMIS Microbiology investigation competency Other services Closed customised PT schemes Trial test materials Scheme development Special bespoke test materials Wish list Other PT services Reference materials Training and education Frequently asked questions Index of analytes 36 SUPS Sugar

3 Introduction LGC Standards - your first choice in proficiency testing Aim of proficiency testing Quality standards Benefits of proficiency testing Who should participate in proficiency testing schemes? Who participates in LGC Standards proficiency testing schemes? Why choose LGC Standards as your proficiency testing provider? Typical scheme cycle from start to finish PORTAL - Proficiency Online Reporting and Trend AnaLysis

4 LGC Standards - your first choice in proficiency testing LGC is an accredited international provider of Proficiency Testing (PT) schemes also known as External Quality Assessment (EQA) schemes. We have over thirty years experience in all aspects of providing PT services to laboratories undertaking chemical, clinical, microbiological, and physical testing as well as measurements. LGC Standards annually runs over 1,500 proficiency testing exercises, serving more than 9,000 laboratories. We produce in excess of 200,000 test materials per annum which are distributed to over 150 countries worldwide. We offer an unprecedented breadth of PT schemes across a wide range of sectors such as food, beverages, environment, clinical, forensics, consumer safety and industrial. In addition to the variety of schemes offered, LGC Standards can also provide managed solutions for in-house proficiency testing providers and training for participants and their customers. 4 Tel: +44 (0) Fax: +44 (0)

5 Aim of proficiency testing Proficiency testing is defined in ISO/IEC as the evaluation of participant performance against pre-established criteria by means of interlaboratory comparisons. The term External Quality Assessment or EQA is often used for proficiency testing in the medical / clinical area. LGC Standards provides a wide range of schemes designed to improve the quality of analytical testing and measurements in those sectors covered. The schemes involve the regular distribution of test materials in order for participants to test for defined parameters, and to have their results statistically analysed. Participation provides laboratories with a means of assessing the accuracy and comparability of their results with peer laboratories over time. When performed within the context of a comprehensive quality assurance programme, proficiency testing is an independent means of assuring the quality of test and calibration results, as described in ISO/IEC and ISO Benefits of proficiency testing There are many benefits associated with participation in a proficiency testing scheme or external quality assessment. Key is the independent assessment of performance and the opportunity to compare your performance with peer laboratories, often on a global basis. Other benefits include: Monitor the quality of measurements made and thereby identify improvements required Greater process control Demonstrate competency to customers, accreditation bodies and other regulatory bodies Identify staff development and training needs Compare methods or procedures Quality standards LGC Standards is committed to continual improvement in quality and efficiency through sound quality assurance procedures. This commitment is demonstrated through certification to ISO 9001 for all our activities. Our PT schemes are accredited by the United Kingdom Accreditation Services (UKAS) certificate number 0001 and operated in accordance with ISO/IEC For details of the accredited schemes, please refer to our scope of accreditation at: Please note that the AFPS, DAH, FIRMS and OIL PT schemes are currently not included. Our proficiency testing services are supported by the scientific excellence of the LGC Group, the UK National Measurement Institute for analytical chemistry, as well as reference materials from LGC Standards, our marketleading manufacturing and distribution business. [email protected] Website: 5

6 Who should participate in proficiency testing schemes? Anyone who needs to independently demonstrate the quality of their analytical results should participate in PT / EQA schemes, because quality of results relates directly to quality of product, reputation in the market and, ultimately, brand value. Whether operating in the food, beverages, environmental, clinical, forensic, pharmaceutical, or other sectors, many regulators view PT schemes as an essential part of quality monitoring and many laboratories link PT results to key performance indicators in the quality assurance process. Who participates in LGC Standards proficiency testing schemes? LGC Standards exports to laboratories in over 150 countries worldwide and our customer base ranges from single small enterprises to inspection organisations of global repute. Our customers include hospitals, clinics, pharmaceutical companies, government agencies, major international food and beverage manufacturers, research organisations, commercial and contract laboratories. At present we annually run over 1,500 proficiency testing exercises, serving more than 9,000 laboratories. Why choose LGC Standards as your proficiency testing provider? We provide high quality, accredited PT schemes with a strong customer base and a proven track record over many years. Additional attributes that position LGC Standards as a global leader in proficiency testing include: A dedicated purpose built PT facility PORTAL Proficiency Online Reporting and Trend AnaLysis tool Rapid turnaround of results reports provided typically between 4 and 10 working days after the result deadline Value for money Schemes tailored to specific sectors Customer service of the highest quality Technical support of in-house experts and external advisors Worldwide sales and logistic network, supporting our customers from local offices in local languages LGC Standards manages a number of bespoke schemes for multi-national companies to meet their particular requirements. These special schemes cover up to 500 laboratories. 6 Tel: +44 (0) Fax: +44 (0)

7 Typical scheme cycle from start to finish Procurement, preparation, dispensing and quality control testing of test materials 6-12 weeks prior Despatch of test materials and instructions to participants Week 1 Round reviewed changes made if required Participants analyse test materials and report results through PORTAL ( as instructed and within the specified deadline Week 2-4 Results analysed and the performance of laboratories compared using appropriate techniques, such as performance scores Week 5 Reports issued and participants notified once report is made available on PORTAL Week 5 Reports are issued as soon as possible after the round closure, although the timescale between closing dates and issue of final report will vary from scheme to scheme. [email protected] Website: 7

8 PORTAL Proficiency Online Reporting Trend AnaLysis PORTAL is a secure web based reporting system available to all participants of LGC Standards PT schemes. It provides an online area from which you can quickly access all of your PT data, including results submission PDF reports, data export and performance trending tools. Benefits include: Easy access to your PT data 24 hours a day Online results submission Complete data traceability Fully secure website with encrypted databases Free support provided by staff based in the UK or your local area Multi-method reporting complete with methodology summaries Multi-analyst reporting Multiple password protected user accounts per laboratory Results submission by analyte or sample Intelligent importing of previously entered data Full control of all data prior to deadlines Customisable performance trends For further information contact: LGC Standards Proficiency Testing 1 Chamberhall Business Park, Chamberhall Green, Bury, Lancashire BL9 0AP, UK Tel +44 (0) Fax +44 (0) [email protected] Web

9 Food and feed PT schemes AFPS Animal feed QCS Chocolate QDCS Dairy chemistry QFCS Food chemistry QGS Gelatine QMAS Meat and fish QMS Food microbiology

10 Food and feed PT scheme selector Scheme Scheme year Number of distributions per scheme year Tests *AFPS Animal feed January - December Four Chemical and microbiological QCS Chocolate January - December Three Chemical and microbiological QDCS Dairy chemistry January - December Four Chemical QFCS Food chemistry January - December Four Chemical QGS Gelatine QMAS Meat and fish QMS Food microbiology January - December Two Microbiological January - December Six Chemical and microbiological January - December Twelve Microbiological *Please note that the AFPS scheme is currently not included in our scope of accreditation. Which international standards are relevant to PT? LGC is accredited by the United Kingdom Accreditation Service (UKAS) for the provision of proficiency testing schemes against ISO/IEC All PT schemes within LGC are operated in accordance with the international standard ISO 13528; copy of our current scope of accreditation is available on our website Tel: +44 (0) Fax: +44 (0)

11 Test material matrix** Analyte group** See page Animal feed (e.g. broiler, cattle, chicken, pig, sheep), calf replacer, premix, oil and lard. Chocolate and cocoa powder. Butter, cheese, cream, milk, milk powder, whey powder, yoghurt and standard solutions. Cereals, cured meat, fruit / vegetable, hard cheese, ready to eat products and standard solutions. Gelatine hydrolysate. Meat, fish and shellfish. Oatmeal and skimmed milk powder, tea, herb and spice. Comprehensive range of chemical and microbiological analysis of animal feeds covering the major analytical areas including proximate analysis, trace elements, aflatoxins, Salmonella spp., and indicator organisms. Chemical parameters of relevance to the chocolate and food testing industries. Microbiological tests including pathogens and indicator organisms. A variety of tests ranging from routine proximate and quality control analysis, regularly performed by dairy and food testing laboratories, to more complex testing such as antibiotics, vitamins and minerals. Nutritional analysis, vitamins and minerals, FAMES, aflatoxins, metals, pesticides, water activity and food additives. Microbiological tests including pathogens and indicator organisms of relevance to gelatine. Proximate analysis and microbiological testing relevant to meat, fish, shellfish and food testing industries. Comprehensive range of microorganisms of relevance to food products, including pathogens, indicator organisms, spoilage organisms and probiotics **Please see current application form and scheme description for accredited and non-accredited status of test materials and analytes. [email protected] Website: 11

12 AFPS - Animal feed The Animal Feeds Proficiency Scheme (AFPS) is specifically designed to meet the needs of laboratories performing chemical or microbiological analysis of animal feedstuffs. Animal feed quality is highly regulated since the majority of animals, or their products, will be converted into food for human consumption. EC Regulation 767/2009 stipulates the requirements for the marketing and use of animal feeds, and (with reference to related regulations) requires complete traceability of feeds, personnel, and additives, and defines the compulsory labelling requirements. Measures must be taken to prevent contamination with hazardous materials above specified limits; such as heavy metals, mycotoxins, etc. and microorganisms capable of causing human disease (zoonotic agents) such as Salmonella species. Major food safety crises can occur from the contamination of animal feed causing risks to animal and human health resulting in recalls; financial damage as large quantities of product have to be destroyed; and the potential for considerable damage to the reputation of any businesses involved. Recalls include melamine in pet foods, numerous events of animal feed cargo rejected due to aflatoxin contamination, pork products contaminated with dioxins, and many more. Preventing these issues through reliable analysis at source backed up by independent verification through participation in a proficiency scheme, can help to save large sums of money for a very small investment. The full range and availability of test materials in AFPS is determined on an annual basis and further details can be found in the AFPS application form and scheme description. Test material Animal feed (e.g. broiler, cattle, chicken, pig, sheep), calf replacer, premix, oil and lard. Analytes Chemical Proximate analysis Acid detergent fibre (ADF), Ash insoluble in hydrochloric acid, Crude ash, Crude fat, Crude fibre, Crude protein, Moisture, Neutral detergent fibre (NDF), Pepsin protein digestibility (PPD), Starch, Sugars. Minerals and trace elements Arsenic, Cadmium, Calcium, Chloride, Chromium, Cobalt, Copper, Iron, Lead, Magnesium, Manganese, Mercury, Phosphorus, Potassium, Selenium, Sodium, Zinc. Fat quality Eluable fat, Fatty acids profile (Absolute), Fatty acids profile (Relative), Free fatty acids, 12:0 Lauric acid, 14:0 Myristic acid, 14:1 n-5 Myristoleic acid, 15:0 Pentadecanoic acid, 16:0 Palmitic acid, 16:1 Palmitoleic acid, 17:0 Heptadecanoic acid, 18:0 Stearic acid, 18:1 cis-9 Oleic acid, 18:2 n-6 Linoleic acid, 18:3 n-3 Linolenic acid, 20:0 Eicosanoic acid, 20:1 Eicosenoic acid, 20:5 n-3 Eicosapentaenoic acid, 22:6 n-3 Docosahexaenoic acid, Insoluble impurities, Moisture, Polymeric triglycerides, Unsaponifiable matter. Aflatoxins Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Ochratoxin, Total aflatoxins. Microbiological Enumeration Enterobacteriaceae, Total viable count, Yeast and Mould. Detection Salmonella species. Note: Test materials and analytes may be added or removed, please see current application form. AFPS is currently not included in our scope of accreditation. 12 Tel: +44 (0) Fax: +44 (0)

13 QCS - Chocolate The Quality in Chocolate Scheme (QCS) is intended for use by microbiologists and chemists working in the chocolate manufacturing industry. Cacao trees were first cultivated by the Aztecs, who used the beans both as a form of currency and to produce a spiced drink known as chocolatl. Over the years, many different types and flavours of chocolate have been developed and cacao derivatives are often blended with other ingredients to produce a diverse range of products. Chocolate is probably the most popular end-product; in some countries up to 90 % of the population buys chocolate on a regular basis. Test material Chocolate Cocoa powder Analytes Chemical Butyric acid, Fat, Moisture, Theobromine, Total nitrogen, Total sugars. Microbiological Enumeration Coliforms, Enterobacteriaceae, Enterococci, Mould, Total aerobic mesophilic count, Yeast. Detection Salmonella species. Chemical Ash, Fat, Moisture, Theobromine. Chocolate products are also one of the few food commodities whose composition is controlled at EC level. For example, EC Directive 2000/36/EC relating to cocoa and chocolate products intended for human consumption, sets common rules and definitions with regard to the composition, manufacture, packaging and labelling of chocolate and cocoa products. Microbiological Enumeration Coliforms, Enterobacteriaceae, Enterococci, Mould, Total aerobic mesophilic count, Yeast. Detection Salmonella species. Note: Test materials and analytes may be added or removed, please see current application form. The full range and availability of test materials in QCS is determined on an annual basis and further details can be found in the QCS application form and scheme description. [email protected] Website: 13

14 QDCS - Dairy chemistry The Quality in Dairy Chemistry Scheme (QDCS) is one of the most comprehensive proficiency testing scheme available to laboratories performing compositional and safety analysis in the dairy sector. EU Regulation 853/2004 introduces specific hygiene rules applying to raw milk and dairy products, including control of residual antibiotics. When cattle ingest aflatoxins and other contaminants in feed, toxic metabolites can be formed and may potentially infect milk, cheese and other dairy products. For laboratories that perform the analysis of dairy produce using traditional wet chemistry techniques, as well as determinations by infrared analysers, participation in a relevant proficiency testing scheme can provide confidence that results of these analyses are meaningful and accurate which, in turn, helps to ensure the safety of these products. The full range and availability of test materials in QDCS is determined on an annual basis and further details can be found in the QDCS application form and scheme description. Test material Butter Cheese Hard and soft. Cream Single, double and whipping. Milk Freeze dried, semi-skimmed, skimmed and whole. Analytes Moisture, ph, Salt. Calcium, Fat, Moisture, ph, Protein, Salt. Fat, Titratable acidity. Milk powder Vitamins A, C, D. Milk powder Skimmed and whole. Whey powder Whey protein concentrate Yoghurt Standard solutions Buffer solution, hydrochloric acid, potassium hydrogen phthalate. Aflatoxin M1, Antibiotics (penicillin and / or cephalosporin, Antibiotics (penicillin and / or sulfur-based), Calcium, Fat, Freezing point, Lactose, Protein, Phosphatase, ph, Titratable acidity, Total solids. Ash, Fat, Insolubility index, Minerals (calcium, potassium, copper, sodium, chloride, iron, magnesium, manganese, phosphorus, zinc), Moisture, Protein, Scorched particles, Titratable acidity, WPNI. Ash, Fat, Lactose, Moisture, Protein, Scorched particles. Ash, Bulk density, Fat, Insolubility index, Lactose, Moisture, ph, Protein. Fat, Protein, Total solids. COD, ph, Titratable acidity. Note: Test materials and analytes may be added or removed, please see current application form. 14 Tel: +44 (0) Fax: +44 (0)

15 QFCS - Food chemistry The Quality in Food Chemistry Scheme (QFCS) is specifically designed to promote the quality and comparability in the measurement of a range of analytes in food products. All kinds of foods are available worldwide and all year round, following years of research and advances in the logistics required. Technology now allows suppliers to harvest, preserve and distribute within a short period of time. However, food quality, consumer satisfaction and government regulations are all issues that need to be taken into consideration with regards to global food trade. Testing these food products in the laboratory is fundamental for safety and stability with respect to microbial growth, chemical / biochemical composition, and physical properties. The full range and availability of test materials in QFCS is determined on an annual basis and further details can be found in the QFCS application form and scheme description. Note: Test materials and analytes may be added or removed, please see current application form. Test material Bread or cake Cereal Edible oil or fat Flour Food Fruit or vegetable Dried tea Nuts Ready to eat products Standard solutions Analytes Nutritional analysis Acidity, Ash, Fat, Moisture, Protein, Total dietary fibre. Vitamins and minerals Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Vitamin B3 (Niacin), Calcium, Chloride, Iron, Sodium. Nutritional analysis Ash, Carbohydrate (total and available), Energy, Fat, Moisture, Phosphate, Protein, Salt, Sodium, Total dietary fibre, Total sugars. Vitamins and minerals Vitamin A (as Retinol), Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Vitamin B3 (Niacin), Vitamin B6 (Pyridoxine), Vitamin B9 (Folic acid), Vitamin B12 (Cobalamins), Vitamin C (as Ascorbic acid), Vitamin D, Vitamin 5 (Pantothenic acid), Iron. Chemical Anisidine value, Colour, Free fatty acids, Iodine value, Peroxide value, Saponification value, Unsaponifiable matter, Water. FAMES 12:0 Lauric acid, 14:0 Myristic acid, 14:1 n-5 Myristoleic acid, 15:0 Pentadecanoic acid, 16:0 Palmitic acid, 16:1 Palmitoleic acid, 17:0 Heptadecanoic acid, 18:0 Stearic acid, 18:1 cis-9 Oleic acid, 18:2 n-6 Linoleic acid, 18:3 n-3 Linolenic acid, 20:0 Eicosanoic acid. Nutritional analysis Ash, Calcium, Fat, Iron, Moisture, Protein. Water activity. Pesticides Herbicides, Organochlorine, Organophosphorus, Synthetic pyrethoids, Triazines. Metals Arsenic, Cadmium, Lead, Mercury, Selenium. Pesticides Herbicides, Organochlorine, Organophosphorus, Syn th e ti c p yr e th o i d s. Aflatoxins Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2. Nutritional analysis Ash, Carbohydrate (total and available), Energy, Fat, Moisture, Phosphate, Protein, Salt, Sodium, Total dietary fibre, Total sugars. Preservatives Benzoic acid (E210 - E213), Sorbic acid (E200 - E203), Sulfur dioxide (E220 - E228). Sweeteners Acesulfame K (E950), Aspartame (E951), Saccharin (E954). Colours Allura red (E129), Brilliant blue (E133), Indigo carmine (E132), Ponceau 4R (E124), Sunset yellow (E110), Tartrazine (E102). [email protected] Website: 15

16 QGS - Gelatine The Quality in Gelatine Scheme (QGS) has been developed in collaboration with the trade body, Gelatine Manufacturers of Europe (GME). GME members account for nearly half of the worldwide gelatine production and the key role of the GME is to ensure that gelatine is manufactured to a consistently high quality for the benefit of gelatine customers and consumers. Test material Gelatine hydrolysate Analytes Enumeration Sulphite-reducing bacteria, Total aerobic mesophilic count. Detection Clostridium perfringens, Coliforms, Escherichia coli, Staphylococcus aureus, Salmonella species. Gelatine is a pure protein obtained from animal raw materials containing collagen and is typically produced in two grades as far as microbiological specification is concerned - food grade and pharmaceutical grade. Note: Test materials and analytes may be added or removed, please see current application form. Gelatine is an excellent nutrient for most bacteria and for this reason the manufacturing processes have to avoid contamination, otherwise the safety and / or quality of the gelatine could be affected. QGS test materials are available on a gelatine hydrolysate matrix in order to represent a realistic challenge, and include the relevant microorganisms for laboratories involved in the quality control analysis of gelatine. The full range and availability of test materials in QGS is determined on an annual basis and further details can be found in the QGS application form and scheme description. 16 Tel: +44 (0) Fax: +44 (0)

17 QMAS - Meat and fish The Quality in Meat and Fish Analysis Scheme (QMAS) is intended for use by chemists and microbiologists working in the meat, fish and shellfish processing industries. Legislation in the form of EU Regulation 882/2004 Official Controls Performed to Ensure the Verification of Compliance with Feed and Food Law, Animal Health and Animal Welfare Rules requires official control laboratories to be accredited to ISO/IEC and therefore to use external means of monitoring performance such as proficiency testing. A variety of real meat test materials in a vacuum packed and lyophilised vial format is provided for participants, enabling them to analyse a comprehensive range of relevant chemical test parameters which are all widely used in the meat sector. The real fish test material allows laboratories to perform a range of basic proximate and heavy metal analysis. The shellfish test material is provided for heavy metal analysis only. Product authentication and speciation is a concern for the fish and meat industries. Our test materials will challenge laboratories carrying out analytical testing to determine the identity of these products and can help give authority to their results. For microbiological determinations, test materials are provided in a lyophilised matrix for the analysis of indicator organisms, as performed routinely in factory based laboratories and third party testing facilities. There are also test materials for pathogen analysis suitable for laboratories permitted to undertake this analysis, often carried out by external providers. The full range and availability of test materials in QMAS is determined on an annual basis and further details can be found in the QMAS application form and scheme description. Note: Test materials and analytes may be added or removed, please see current application form. Test material Meat Dried and cured meat Precooked, processed and raw meat Fish Shellfish Analytes Chemical Hydroxyproline, Nitrate*, Nitrite*, Saturates, Mono-unsaturated fats, Poly-unsaturated fats, Total fat, Total trans fatty acids. Trace elements Arsenic, Cadmium, Lead, Mercury. Meat authenticity. Microbiological Enumeration Coagulase-positive staphylococci, Coliforms, Clostridium perfringens, Enterobacteriaceae, Escherichia coli, Lactic acid bacteria, Mould, Pseudomonas, Sulphite-reducing clostridia, Total aerobic mesophilic count, Yeast. Detection Campylobacter species*, Listeria species, Listeria monocytogenes, Salmonella species. *Lyophilised vial format. Chemical Ash, Carbohydrate, Dietary fibre, Energy, Fat, Moisture, ph, Phosphate, Protein, Salt, Sodium, Total sugars. Chemical Ash, Calcium, Carbohydrate, Dietary fibre, Energy, Fat, Moisture, ph, Phosphate, Potassium, Protein, Salt, Sodium, Total sugars. Chemical Ash, Fat, Moisture, ph, Protein, Salt, Arsenic, Cadmium, Lead, Mercury, Histamine, Trimethylamine (TMA), Total volatile nitrogen (TVN). Fish authenticity. Fish speciation. Microbiological Enumeration Coagulase-positive staphylococci, Enterobacteriaceae, Escherichia coli, Total aerobic mesophilic count. Detection Salmonella species. Trace elements Arsenic, Cadmium, Lead, Mercury. Microbiological Enumeration Coagulase-positive staphylococci, Enterobacteriaceae, Escherichia coli, Total aerobic mesophilic count. Detection Salmonella species. [email protected] Website: 17

18 QMS - Food microbiology The Quality in Microbiology Scheme (QMS) is intended for use by microbiologists working in the food, beverage, dairy, herbs, spice and many other industries. Food testing is an essential element of the Hazard Analysis Critical Control Point (HACCP) process in food production as it verifies the controls are working at the critical points in manufacturing. Failures leading to food poisoning outbreaks can have a devastating effect on reputation, brand value and ultimately profits. Failures which do not lead to an outbreak also can have an effect, leading to reduced shelf life and wastage, or discernible product defects - affecting consumer confidence in the product. Laboratories carrying out such microbiological testing need to be able to demonstrate that they are producing accurate and meaningful results. This can be done by conducting a comprehensive quality assurance programme, which includes regular participation in a suitable PT scheme. Test material Oatmeal and skimmed milk powder Herbs Analytes Enumeration Aerobic psychrotrophs, Bacillus cereus, Bifidobacterium species*, Campylobacter species*, Coagulase positive-staphylococci, Coliforms, Clostridium perfringens, Enterobacteriaceae, Enterococci (faecal streptococci), Escherichia coli, Lactic acid bacteria, Listeria monocytogenes, Listeria species, Pseudomonas species, Salmonella species, Thermotolerant coliforms, Anaerobic sulphite-reducing bacteria, Mesophilic aerobic spores, Mesophilic anaerobic spores, Thermophilic aerobic spores, Total aerobic mesophilic count, Total anaerobic mesophilic count, Yeast, Mould, Yeast and mould, Osmophilic yeast, Osmophilic mould. Detection Campylobacter species*, Clostridium species, Coliforms, Cronobacter species, Enterobacteriaceae, Escherichia coli, Escherichia coli O157 (non toxigenic strain), Listeria monocytogenes, Listeria species, Salmonella species, Shigella species, Vibrio parahaemolyticus, Vibrio species, Yersinia enterocolitica. *Lyophilised vial format. Detection Salmonella species. The range of organisms offered for testing covers all those that are likely to be found, from spoilage organisms, pathogens, indicator organisms and normal background flora. The full range and availability of test materials in QMS is determined on an annual basis and further details can be found in the QMS application form and scheme description. Spice Tea Detection Salmonella species. Enumeration Coagulase positive-staphylococci, Coliforms, Total aerobic mesophilic count, Yeast and mould. Detection Salmonella species. Note: Test materials and analytes may be added or removed, please see current application form. 18 Tel: +44 (0) Fax: +44 (0)

19 Water and environment PT schemes AIR PT Workplace air, ambient air, indoor / chamber air and stack emissions AQUACHECK Water, agricultural soils and sludges CONTEST Contaminated land QWAS Water microbiology

20 Water and environment PT scheme selector Scheme Scheme year Number of distributions per scheme year Tests AIR PT Workplace, ambient, indoor / chamber and stack emissions AQUACHECK Water, agricultural soils and sludges CONTEST Contaminated land QWAS Water microbiology April - March Six Chemical and physical April - March Twenty Chemical, ecotoxicology, physical and radiochemical April - March Five Chemical and physical January - December Ten Microbiological How often do I need to participate? The frequency that a laboratory needs to participate in proficiency testing depends on a wide range of factors specific to each individual laboratory. Therefore every individual laboratory may have a different need, which is why our schemes provide flexible participation, although some do have a minimum participation level. Third parties, such as regulatory bodies, may recommend minimum levels of participation. To gain the benefit from trend analysis, participation in a minimum of four rounds over a scheme year is normally recommended Tel: +44 (0) Fax: +44 (0)

21 Test material matrix** Analyte group** See page Filters, sorbent tubes and stack / impinger solutions. Quartz, organic and elemental analytes at a range of concentrations. 22 Clean waters and waste waters, agricultural soils and sewage sludge. Soil extracts, soil materials, solid waste and standard solutions. Waters (e.g. bathing, environmental, mineral, potable, process, recreational, sea, surface, waste) and simulated effluent sludge. Inorganic, organic and metal analytes in a range of water types. Determination of radiochemical and ecotoxicological parameters. Inorganic, organic and metal analytes measured in soil, leachates and solutions from contaminated land. Routine microbiological testing, complex pathogen detection, and indicator organisms **Please see current application form and scheme description for accredited and non-accredited status of test materials and analytes. [email protected] Website:

22 AIR PT - Workplace air, ambient air, indoor / chamber air and stack emissions The AIR Proficiency Testing scheme is a collaboration between LGC and the UK Health and Safety Laboratory (HSL). The AIR PT scheme is run by LGC and is supported by the technical expertise at HSL. The scheme combines HSL s WASP and LGC s STACKS schemes, providing an integrated scheme that is specifically designed for all laboratories undertaking analysis of samples derived from various fields of air monitoring. A common approach to monitor air quality is to collect gaseous and particulate pollutants on to suitable sampling media and submit these to the laboratory for analysis. The aim of the AIR PT scheme is to support the quality of analytical data generated by laboratories performing chemical analysis of the samples taken. The test materials in the AIR PT scheme may contain one or more analytes prepared at various concentrations on appropriate sampling media. They are prepared in such a way as to give a realistic test for the laboratory and a valuable means of monitoring analytical performance. The AIR PT scheme will support the quality of this analytical data generated by laboratories performing measurements on samples taken from, for example, ambient, indoor, stack and workplace air testing arenas. Regular monitoring allows information to be collected, in order to review and assess air quality and ensure legislative standards are being met. It also helps to determine the effectiveness of control systems designed to prevent air pollution. The full range and availability of test materials in AIR PT is determined on an annual basis and further details can be found in the AIR PT application form and scheme description. Test material Workplace air Filters and sorbent tubes. Ambient air Filters and sorbent tubes. Indoor / Chamber air Sorbent tubes. Impinger solutions Quartz filter Containing trace elements and fly ash. Dust Solution. Dust Filter. Analytes Cadmium, Chromium, Cobalt, Copper, Iron, Lead, Manganese, Nickel, Zinc. Mass of solids, Respirable grade quartz. 1,1,1,-trichloroethane, Benzene, Ethyl benzene, Formaldehyde 2,4-DNPH derivative, n-butyl acetate, n-hexane, Tetrachloroethene, Toluene, Trichloroethene, Xylene (all isomers). Arsenic, Benzene, Cadmium, Ethyl benzene, Lead, Nickel, Nitrogen dioxide (as nitrite), Toluene, Xylene (all isomers). 124-TMB, 4-PCH, a-pinene, Benzene, Butyl acetate, Cyclohexanone, Dodecane, Limonene, Methyl IsoButyl Ketone (MIBK), n-hexane, Phenol, p-xylene, Toluene. Ammonia, Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Hydrogen chloride (Hydrogen chloride and HCl), Hydrogen fluoride, Lead, Manganese, Mercury, Nickel, Sulfur dioxide, Thallium, Vanadium. Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Nickel, Thallium, Vanadium. Total solids. Mass of solids. Note: Test materials and analytes may be added or removed, please see current application form. 22 Tel: +44 (0) Fax: +44 (0)

23 AQUACHECK - Water, agricultural soils and sludges Everybody depends on water; it is vital in sustaining all natural systems on and under the earth s surface. Water sources require regular analyses to determine their safety and suitability for a variety of uses. The water we drink must be good in quality and quantity so that it is free from harmful chemicals and microorganisms in order to guarantee wellbeing. The water discharged by local waste water treatment plants and industry must be monitored to ensure strict compliance with environmental guidelines. Process waters must be kept clean from contaminants to ensure product quality. As water sources can change, regular testing is advised. The types of analysis could vary from simple field testing for a single analyte to complex laboratory based analysis. The AQUACHECK scheme provides test materials for the analysis of organic, inorganic chemicals and trace elements in clean waters; waste waters and effluents; agricultural soils; sewage sludge; ecotoxicology, radiochemistry and trial test materials. Different test materials are provided for different analytical groups: major inorganic compounds; metals; phenols; organochlorine pesticides and many others. Participants may select any number or combination of groups to suit their analytical requirements. Test parameters within the groups are continually reviewed to ensure they meet current laboratory testing and regulatory requirements. Test materials are distributed frequently throughout the scheme year. The use of natural matrices ensures that matrix effects are fully represented. Where it is not possible to use natural matrices, the AQUACHECK scheme uses either a synthetic matrix or a simulated material which represents some of the matrix effects likely to occur in real samples. In this way participation in AQUACHECK enables laboratories to monitor and improve the quality of their routine analytical measurements and provides independent evidence of laboratory performance for both laboratory management and customers. It also allows laboratories to demonstrate to regulators and accreditation bodies the validity of their analytical measurements. The full range and availability of test materials in AQUACHECK is determined on an annual basis and further details can be found in the AQUACHECK application form and scheme description. [email protected] Website: 23

24 Test material Analytes Test material Analytes Sample 1H Major inorganic components: (In hard water). Sample 1S Major inorganic components: (In soft water). Sample 1A Major ions in higher salinity potable water. Sample 2H Nutrients and others: (In hard water). Sample 2S Nutrients and others: (In soft water). Sample 2A ph in poorly buffered waters. Sample 3 Non-specific analytes in clean water. Sample 3A Inorganic disinfection by-products in clean water. Sample 3B Free chlorine in clean water. Sample 3C Total chlorine in clean water. Sample 4 Metals in surface water: (Preserved in 0.5 % Nitric acid). Alkalinity, Barium, Calcium, Chloride, Conductivity (20ºC), Fluoride, Kjeldahl Nitrogen, Magnesium, Potassium, Sodium, Sulfate, Total hardness, Total phosphorus. Alkalinity, Barium, Calcium, Chloride, Conductivity (20ºC), Fluoride, Kjeldahl Nitrogen, Magnesium, Potassium, Sodium, Sulfate, Total hardness, Total phosphorus. Chloride, Conductivity (20ºC), Magnesium, ph at 20-25ºC, Sodium, Sulfate, Total dissolved solids (180ºC), Total organic carbon (TOC). Ammonia, Colour, Conductivity (20ºC), Free cyanide, Nitrate, Nitrite, Permanganate index (PI), ph at 20-25ºC, Silicate, Soluble reactive phosphorus, Total cyanide, Total dissolved solids (180ºC), Total oxidised nitrogen (TON). Ammonia, Colour, Conductivity (20ºC), Free cyanide, Nitrate, Nitrite, Permanganate index (PI), ph at 20-25ºC, Silicate, Soluble reactive phosphorus, Total cyanide, Total dissolved solids (180ºC), Total oxidised nitrogen (TON). ph at 20-25ºC - Low ph at 20-25ºC - High BOD (5 day), COD, Dissolved organic carbon, Methylene blue active substances (MBAS), Non-ionic surfactants, Suspended solids, Turbidity. Bromate, Bromide, Chlorate (high level), Chlorate (low level), Chlorite (high level), Chlorite (low level). Free chlorine. Total chlorine. Aluminium, Barium, Boron, Copper, Iron, Lithium, Manganese, Silver, Strontium, Zinc. Sample 5G Toxic metals in groundwater: (Preserved in 0.5 % Nitric acid). Sample 6A Haloforms and chlorinated solvents in clean water. Sample 6B Phenols in clean water. Sample 6C Benzene, toluene and xylenes in clean water. Sample 7A Organochlorine pesticides in clean water. Sample 7B Chlorinated solvents in clean water. Sample 7C Polycyclic aromatic hydrocarbons: (2 spikes) in clean water. Antimony, Arsenic, Beryllium, Cadmium, Chromium, Cobalt, Lead, Mercury, Molybdenum, Nickel, Selenium, Tin, Vanadium. Bromodichloromethane, Bromoform, Carbon tetrachloride, Chloroform, Dibromochloromethane, Tetrachloroethene, Trichloroethene, 1,2-Dichloroethane. 2,4,6-Trichlorophenol, 2,4-Dichlorophenol, 2,5-Dimethylphenol, 2-Chlorophenol, 2-Methylphenol (o-cresol), 3,5-Dimethylphenol, 3-Bromophenol, 3-Methylphenol (m-cresol), 4-Chlorophenol, 4-Methylphenol (p-cresol), Pentachlorophenol, Phenol, Total monosubstituted methylphenols. Benzene, Ethylbenzene, m-+ p-xylene, m-xylene, o-xylene, p-xylene, Styrene, Toluene, Total Xylene. Aldrin, Alpha endosulphan, Alpha hexachlorocyclohexane, Beta endosulphan, Beta hexachlorocyclohexane, Delta hexachlorocyclohexane, Dieldrin, Endrin, Heptachlor, Heptachlor epoxide, Hexachlorobenzene, Lindane (Gamma HCH), o,p -DDD (TDE), o,p -DDE, o,p-ddt, p,p -DDD, p,p -DDE, p,p -DDT, Pendimethalin, Pentachlorobenzene, Trifluralin. 1,1,1-Trichloroethane, 1,2,3-Trichlorobenzene, 1,2,4-Trichlorobenzene, 1,3,5-Trichlorobenzene, 1,2-Dichloroethane, Carbon tetrachloride, Chloroform, Hexachlorobutadiene, Tetrachloroethene, Trichloroethene. Spiking solution 7C1: Benz(a)pyrene, Benzo(b)fluoranthene, Benzo(ghi)perylene, Benzo(k)fluoranthene, Fluoranthene, Indeno(1,2,3-cd)pyrene. Spiking solution 7C2: Acenaphthene, Acenaphthylene, Anthracene, Benz(a)anthracene, Chrysene, Dibenz(ah) anthracene, Fluorene, Naphthalene, Perylene, Phenanthrene, Pyrene. Sample 4G Metals in groundwater: (Preserved in 0.5 % Nitric acid). Sample 5 Toxic metals in surface water: (Preserved in 0.5 % Nitric acid). Sample 5A Metals for hydride generation in clean water: (Preserved in 0.5 % hydrochloric acid). Sample 5B EQS metals in clean water: (Preserved in 0.5 % Nitric acid). Sample 5C Chromium (VI) in clean water. Aluminium, Barium, Boron, Copper, Iron, Lithium, Manganese, Silver, Strontium, Zinc. Antimony, Arsenic, Beryllium, Cadmium, Chromium, Cobalt, Lead, Mercury, Molybdenum, Nickel, Selenium, Tin, Vanadium. Antimony, Arsenic, Selenium, Tin. Cadmium, Copper, Lead, Mercury, Nickel, Total chromium, Vanadium, Zinc. Chromium (VI). Sample 7D Polychlorinated biphenyls in clean water. Sample 8 Acid herbicides in clean water: (2 spikes). Sample 8B Triazines and urea herbicides in clean water: (2 spikes). PCB (28), PCB (52), PCB (101), PCB (118), PCB (138), PCB (153), PCB (180). Spiking solution 8(1): 2,3,6-TBA, 2,4,5-T, 2,4-D, 2,4-DB, Benazolin, Bentazone, Bromacil, Bromoxynil, Clopyralid, Dicamba, Dichlobenil, Dichlorprop, Fluroxypyr, Ioxynil, MCPA, MCPB, Mecoprop, Metaldehyde, Metazachlor, Propachlor, Propyzamide, Trichlopyr. Spiking solution 8(2): AMPA, Glyphosate. Spiking solution 8B(1): Chlortoluron, Diflufenican, Diuron, Isoproturon, Linuron metamitron, Methabenzthiazuron, Monuron. Spiking solution 8B(2): Ametryn, Atrazine, Carbetamide, Cyanazine, Pirimicarb, Prometryn, Propazine, Simazine, Terbutryn, Trietazine. Note: Test materials and analytes may be added or removed, please see current application form. Note: Test materials and analytes may be added or removed, please see current application form. 24 Tel: +44 (0) Fax: +44 (0)

25 Test material Sample 9 Organophosphorus pesticides in clean water. Sample 10 Nutrients and other analytes in wastewater. Sample 11 Non-specific analytes in wastewater. Sample 12 Metals in wastewater: (Preserved in 0.5 % Nitric acid). Sample 12C Chromium (VI) in wastewater. Sample 13 Sewage sludge inorganics and specific elements. Sample 14 Agricultural soil inorganics and specific elements. Sample 15 Settleable solids in wastewater. Sample 16 Compositional analysis of sewage sludge. Sample 17A Major wastewater analytes. Sample 17B Total phenol, cyanide and sulfate in wastewater. Sample 17C Metals in wastewater: (Preserved in 0.5 % Nitric acid). Sample 17D Ammonia, phosphate and nitrogen in wastewater. Sample 18A Haloforms and chlorinated solvents in wastewater. Sample 18B Phenols in wastewater. Analytes Azinphos-ethyl, Azinphos-methyl, Chlorfenvinphos, Chlorpyrifos, Cypermethrin, Diazinon, Dichlorvos, Fenitrothion, Fenthion, Malathion, Mevinphos, Parathion-ethyl, Parathion-methyl, Propetamphos. Ammonia, Chloride, Free cyanide, Kjeldahl nitrogen, Nitrate, Nitrite, Silicate, Soluble reactive phosphorus, Total cyanide, Total oxidised nitrogen (TON), Total nitrogen, Total phosphorus. BOD (5 day), COD, Dissolved / Total organic carbon, Methylene blue active substances (MBAS), Suspended solids, Turbidity. Aluminium, Antimony, Arsenic, Cadmium, Chromium, Copper, Iron, Lead, Manganese, Mercury, Molybdenum, Nickel, Selenium, Tellurium, Uranium, Zinc. Chromium (VI). Arsenic, Cadmium, Chromium, Cobalt, Copper, Fluoride, Iron, Lead, Manganese, Mercury, Molybdenum, Nickel, Selenium, Total boron, Total nitrogen, Total phosphorus, Total potassium, Vanadium, Zinc. Arsenic, Cadmium, Carbonate content, Chromium, Cobalt, Conductivity, Copper, Extractable phosphorus, Extraction of magnesium, Extraction of potassium, Extraction of sodium, Fluoride, Iron, Lead, Loss on ignition, Manganese, Mercury, Molybdenum, Nickel, Organic carbon content, ph at 20-25ºC, Selenium, Total boron, Total nitrogen, Total phosphorus, Total potassium, Total solids, Vanadium, Water extractable boron, Zinc. Settleable solids. Calcium, Loss on ignition (500 ± 5ºC), Magnesium, ph at 20-25ºC, Total solids (105 ± 5ºC). Conductivity (20ºC), Non filterable COD, ph at 20-25ºC, Salinity, Settled COD, Suspended solids, Total COD, Total dissolved solids (180ºC). Cyanide, Sulfate, Total phenol. Aluminium, Antimony, Arsenic, Barium, Boron, Cadmium, Chromium, Cobalt, Copper, Iron, Lead, Manganese, Molybdenum, Mercury, Nickel, Selenium, Silver, Tin, Vanadium, Zinc. Ammonia, Soluble reactive phosphorus (PO 4 ), Total nitrogen, Total phosphorus. 1,2-Dichloroethane, Bromodichloromethane, Bromoform, Carbon Tetrachloride, Chloroform, Dibromochloromethane, Tetrachloroethene, Trichloroethene. 2,4,6-Trichlorophenol, 2,4-Dichlorophenol, 2,5-Dimethylphenol, 2-Chlorophenol, 2-Methylphenol (o-cresol), 3,5-Dimethylphenol, 3-Bromophenol, 3-Methylphenol (m-cresol), 4-Chlorophenol, 4-Methylphenol (p-cresol), Pentachlorophenol, Phenol, Total monosubstituted methylphenols. Test material Sample 18C Benzene, toluene and xylenes in wastewater. Sample 19A Organochlorine pesticides in wastewater. Sample 19B Chlorinated solvents in wastewater. Sample 19C Polycyclic aromatic hydrocarbons: (2 spikes) in wastewater. Sample 19D Polychlorinated biphenyls: (PCBs) in wastewater. Sample 20 Acid herbicides: (2 spikes) in wastewater. Sample 20B Triazines, and urea herbicides: (2 spikes) in wastewater. Sample 21 Organophosphorus pesticides in wastewater. Analytes Benzene, Ethylbenzene, m-+ p-xylene, m-xylene, o-xylene, p-xylene, Styrene, Toluene, Total Xylene. Aldrin, Alpha endosulphan, Alpha hexachlorocyclohexane (HCH), Beta endosulphan, Beta hexachlorocyclohexane (HCH), Delta hexachlorocyclohexane (HCH), Dieldrin, Endrin, Heptachlor, Heptachlorepoxide, Hexachlorobenzene, Lindane (Gamma HCH), o,p -DDD (TDE), o,p -DDE, o,p-ddt, p,p -DDD, p,p -DDE, p,p -DDT, Pendimethalin, Pentachlorobenzene, Trifluralin. 1,2-Dichloroethane, 1,1,1-Trichloroethane, 1,2,3-Trichlorobenzene, 1,2,4-Trichlorobenzene, 1,3,5-Trichlorobenzene, Carbon tetrachloride, Chloroform, Hexachlorobutadiene, Tetrachloroethene, Trichloroethene. Spiking solution 19C1: Benz(a)pyrene, Benzo(b)fluoranthene, Benzo(ghi)perylene, Benzo(k)fluoranthene, Fluoranthene, Indeno(1,2,3-cd)pyrene. Spiking solution 19C2: Acenaphthene, Acenaphthylene, Anthracene, Benz(a)anthracene, Chrysene, Dibenz(ah) anthracene, Fluorene, Naphthalene, Perylene, Phenanthrene, Pyrene. PCB (28), PCB (52), PCB (101), PCB (118), PCB (138), PCB (153), PCB (180). Spiking solution 20(1): 2,3,6-TBA, 2,4,5-T, 2,4-D,2, 4-DB, Benazolin, Bentazone, Bromacil, Bromoxynil, Clopyralid, Dicamba, Dichlobenil, Dichlorprop, Fluroxypyr, Ioxynil, MCPA, MCPB, Mecoprop, Metaldehyde, Metazachlor, Propachlor, Propyzamide,Triclopyr. Spiking solution 20(2): AMPA, Glyphosate. Spiking solution 20B(1): Chlortoluron, Diflufenican, Diuron, Isoproturon, Linuron, Metamitron, Methabenzthiazuron, Monuron. Spiking solution 20B(2): Ametryn, Atrazine, Carbetamide, Cyanazine, Pirimicarb, Prometryn, Propazine, Simazine, Terbutryn, Trietazine. Azinphos-ethyl, Azinphos-methyl, Chlorfenvinphos, Chlorpyrifos, Cypermethrin, Diazinon, Dichlorvos, Fenitrothion, Fenthion, Malathion, Mevinphos, Parathion-ethyl, Parathion-methyl, Propetamphos. Note: Test materials and analytes may be added or removed, please see current application form. Note: Test materials and analytes may be added or removed, please see current application form. [email protected] Website: 25

26 Test material Sample 22 Qualitative organics by GCMS in clean water. Sample 22A Qualitative organics by purge, trap and / or headspace GCMS in clean water. Analytes Ten organic analytes are provided for qualitative identification. This sample is designed to test the ability of laboratories to identify organic compounds via GCMS analysis. Participants are provided with a solution containing ten organic compounds. The test requires that participants identify the ten compounds present. Results returned will be identified as satisfactory or unsatisfactory. Participants are also provided with a solvent blank. The choice of the ten organic compounds is designed to avoid the formation of reaction by-products. Six organic analytes are provided for qualitative identification. This sample is designed to test the ability of laboratories to identify organic compounds via purge, trap and / or headspace GCMS analysis. Participants are provided with a solution containing six organic compounds. The test requires that participants identify the six compounds present. Results returned will be identified as satisfactory or unsatisfactory. Participants are also provided with a solvent blank. Test material Sample 30 Gross alpha and gross beta in clean water. Sample 31 Aqueous tritium in clean water. Sample 32 Total sulfide in wastewater. Sample 33 Chlorophyll a in clean water. Sample 50 Ecotoxicology. Analytes Gross alpha as 241 Americium, Gross alpha as 239 Plutonium, Gross alpha as 230 Thornium Gross beta as 137 Caesium, Gross beta as 40 Potassium, Gross beta as 90 Strontium. Aqueous tritium. Total sulfide. Chlorophyll a. 15 minute luminescent bacteria IC50 tests, Daphnia magna 24 hr EC50, Daphnia magna 48 hr EC50, Freshwater algae growth inhibition test biomass reduction (Pseudokirschneriella subcapitata), Other 30 min luminescent bacteria IC50 tests, Vibrio fischeri 30 minute IC50 (ISO ). Note: Test materials and analytes may be added or removed, please see current application form. Sample 23 Mineral oil in wastewater. Sample 24 Oil and grease in wastewater. Sample 25 Qualitative determination in clean water. The choice of the six organic compounds is designed to avoid the formation of reaction by-products. Total hydrocarbons by GC analysis, Total hydrocarbons by Gravimetric analysis, Total hydrocarbons by IR analysis, Volume of sample provided. Total oil and grease, Volume of sample provided. The intent of this sample is to test the ability of laboratories to detect and identify an unknown contaminant in surface / potable waters. This sample is designed for laboratories which may be involved in investigating potentially contaminated potable or surface waters and tests both the extraction and identification stages of investigations. Participants are provided with a two litre water sample and one or more indicators of a potential problem, e.g. water is discoloured or has an oily sheen. Sample 26 PFOS and PFOA in clean water. Sample 27 AOX in wastewater. Sample 28 Formaldehyde in clean water. Sample 29 High and low COD. Participants are asked to identify the contaminating substance(s). Results returned will be identified as satisfactory or unsatisfactory. Perfluorooctanoic acid, Perflurooctanesulfonic acid. Adsorbable organically bound halogens. Formaldehyde. COD - high, COD - low. Note: Test materials and analytes may be added or removed, please see current application form. 26 Tel: +44 (0) Fax: +44 (0)

27 CONTEST - Contaminated land Land contamination can pose both environmental and human health risks. The main causes of contamination are direct discharge of industrial wastes, domestic pollution, over-usage of pesticides, oil and fuel dumping, leaching of wastes from landfills and leaking underground storage tanks which corrode over time releasing toxic substances into previously clean soils. Participation in a proficiency testing scheme for soil, such as CONTEST, is a requirement of the UK Environment Agency s Monitoring Certification Scheme (MCERTS) Performance Standard for Laboratories Undertaking Chemical Testing of Soil. The scheme includes a comprehensive range of analytes of relevance to the testing of soils. The test materials are available as standard solutions, soil extracts and soil materials for the analysis of metals, inorganic contaminants, organics, soil leachates and solid waste for Waste Acceptance Criteria (WAC) in accordance with EN :2002, 10:1 single stage leaching test. The full range and availability of test materials in CONTEST is determined on an annual basis and further details can be found in the CONTEST application form and scheme description. Note: Test materials and analytes may be added or removed, please see current application form. Test material Polyaromatic hydrocarbons (PAHs) Soil and standard solution. Polychlorobiphenyls (PCBs) Soil and standard solution. Total petroleum hydrocarbons (TPH) Soil and standard solution. Total petroleum hydrocarbons (TPH) Volatile range: Soils and standard solution. Chlorinated hydrocarbons Soil and standard solution. BTEX Soil and standard solution. Phenols Soil and standard solution. Semi-volatile organic compounds (SVOC) Standard solution. Miscellaneous Soil. Metals Soil, acid extract of soil and standard solution. Leachable contaminants Soil. Waste Acceptance Criteria (EN :2002 (10:1) single stage leaching test) Leachate material and solid waste. Inorganic contaminants Soil. Inorganic contaminants Standard solution. Analytes Acenaphthene, Acenaphthylene, Anthracene, Benz(a)anthracene, Benz(a)pyrene, Benzo(b)fluoranthene, Benzo(k)fluoranthene, Benzo(b/k)fluoranthene (sum), Benzo(ghi)perylene, Chrysene, Dibenz(ah)anthracene, Fluoranthene, Fluorene, Indeno(123-cd)pyrene, Naphthalene, Phenanthrene, Pyrene, Total PAH. PCB (28), PCB (52), PCB (101), PCB (118), PCB (138), PCB (153), PCB (180). Total TPH (C 10 -C 40 inclusive), TPH Aliphatic >C 10 -C 12, TPH Aliphatic >C 12 -C 16, TPH Aliphatic C 16 -C 21, TPH Aliphatic >C 21 -C 35, TPH Aliphatic >C 35 -C 40, TPH Aromatic >EC 10 -EC 12, TPH Aromatic >EC 12 -EC 16, TPH Aromatic >EC 16 -EC 21, TPH Aromatic >EC 21 -EC 35, TPH Aromatic >EC 35 -EC 40. TPH, Aliphatic C 6, TPH, Aliphatic >C 6 -C 8, TPH, Aliphatic >C 8 -C 10, TPH, Aromatic C 6, TPH, Aromatic >C 6 -C 8, TPH, Aromatic >C 8 -C 10. 1,1,1,2-Tetrachloroethane, 1,1,1-Trichloroethane, 1,2-Dichloroethane, 1,2-Dichloroethene, 2-Chlorotoluene, Chlorobenzenes (sum of 1,2,4- and 1,2,3-trichlorobenzene), Dichloromethane, Hexachloro-1,3-butadiene, Tetrachloromethane, Trichloroethene, Trichloromethane, Vinyl chloride. Benzene, Ethylbenzene, m- + p-xylenes, o-xylene, Toluene. Cresols, Phenol, Xylenols, Distillable phenolic substances, Monohydric phenols, Total cresols, Total phenol (sum of Cresols, Phenol, Xylenols), Total xylenols. SVOCs (Qualitative identification). Elemental sulfur, Total organic carbon. Antimony, Arsenic, Barium, Beryllium, Cadmium, Chromium, Chromium (VI), Cobalt, Copper, Iron, Lead, Manganese, Mercury, Molybdenum, Nickel, Selenium, Thallium, Tin, Vanadium, Zinc. Antimony, Ammonia, Arsenic, Barium, Boron, Cadmium, Calcium, Chloride, Chromium, Chromium (VI), COD, Conductivity (20 C), Copper, Fluoride, Free cyanide, Iron, Lead, Magnesium, Mercury, Molybdenum, Nickel, Nitrate, ph, Phenol index, Phosphate, Potassium, Selenium, Sodium, Sulfate, Thiocyanate, Tin, TOC/DOC, Total cyanide, Zinc. Antimony, Arsenic, Barium, Cadmium, Chloride, Chromium, Copper, DOC, Fluoride, Lead, Mercury, Molybdenum, Nickel, Phenol index, Selenium, Sulfate, Total dissolved solids, Zinc. Ammonia, Complex cyanide, Dry matter, Easily liberated sulfide, Free cyanide, Loss on ignition, ph, Thiocyanate, Total cyanide, Total sulfate, Water soluble (boron, chloride, fluoride, sulfate). Easily liberated sulfide, Thiocyanate, Total cyanide, Total fluoride, Total sulfate, Water soluble boron. [email protected] Website: 27

28 QWAS - Water microbiology The Quality in Water Analysis Scheme (QWAS) scheme is solely intended for microbiological analysis of a wide range of water samples, effluents and sludges. QWAS can help laboratories to meet the requirements of the laboratory standard ISO/IEC 17025, which states that analytical results must be monitored using external quality assessment and the EU Directive 98/83/EC dealing with the quality of water for human consumption. For laboratories that perform the analysis of water, participation in a relevant proficiency testing scheme can provide confidence that results of these analyses are meaningful and accurate which, in turn, helps to ensure the safety of water. The full range and availability of test materials in QWAS is determined on an annual basis and further details can be found in the QWAS application form and scheme description. Test material Bathing, recreational and surface water Bathing, surface and wastewater Effluent sludge Environmental water Mineral water Potable water Process water Sea water Analytes Enumeration Coagulase-positive staphylococci, Sulphite-reducing clostridia. Enumeration Faecal coliforms, Enterococci (faecal streptococci), Escherichia coli, Total coliforms. Detection Salmonella species. Enumeration Escherichia coli. Detection Salmonella species. Enumeration Legionella species by culture, Legionella species by PCR, Legionella pneumophila by culture, Legionella pneumophila by PCR. Detection Legionella species Legionella pneumophila Identification Legionella species Legionella pneumophila Enumeration Enterococci (faecal streptococci), Escherichia coli, Pseudomonas aeruginosa, Total aerobic count at 22 C, Total aerobic count at 37 C. Detection Coagulase-positive staphylococci, Sulphite-reducing clostridia, Sulphite-reducing clostridia spores ONLY. Enumeration Clostridium perfringens, Coliforms, Enterococci (faecal streptococci), Escherichia coli, Pseudomonas aeruginosa, Sulphite-reducing clostridia, Sulphite-reducing clostridia spores ONLY, Total aerobic count at 22 C, Total aerobic count at 37 C. Detection Coliforms, Enterococci (faecal streptococci), Escherichia coli, Legionella species, Legionella species (low levels) Sulphite-reducing clostridia. Enumeration Pseudomonas species, Total aerobic count, Yeast, Mould, Yeast and mould. Enumeration Faecal coliforms, Enterococci (faecal streptococci), Escherichia coli, Total coliforms. Detection Salmonella species. Note: Test materials and analytes may be added or removed, please see current application form. 28 Tel: +44 (0) Fax: +44 (0)

29 Beverage PT schemes BAPS Brewing analytes DAPS Alcoholic drinks MAPS Malt analytes QBS Soft drinks and fruit juices SUPS Sugar

30 Beverage PT scheme selector Scheme Scheme year Number of distributions per scheme year BAPS Brewing analytes January - December Twelve - Chemistry Six - Microbiology Twelve - Sensory Tests Chemical, microbiological and sensory DAPS Alcoholic drinks January - December Four Chemical MAPS Malt analytes January - December Twelve Chemical and physical QBS Soft drinks and fruit juices January - December Four Chemical and microbiological SUPS Sugar January - December Twelve Chemical and microbiological How will I receive my report? Following statistical evaluation of the results, the reports will generally be available on the website within 4 to 10 working days of round closure (see specific scheme description). Participants will be ed when the report is available. The content of reports vary from scheme to scheme but include details of the composition of test materials, the assigned values, and tabular and / or graphical representations of participants results. Paper copies are also available for an additional charge Tel: +44 (0) Fax: +44 (0)

31 Test material matrix** Analyte group** See page Ales, lagers, brewing liquor and effluent solution. Distilled spirits, fermented worts, ciders, wines and fortified wines, liqueurs / cream liqueurs, and other alcoholic beverages. Brewing / distilling malted barley, barley, malt flour and black / crystal malt. Carbonated drink, carbonated drink (degassed), dilutable / ready to drink fruit juice, soft drink and apple juice. Cane or beet sugar, raw sugar, molasses and Stevia. Routine and complex chemical tests of relevance to the brewing industry for quality control and product characterisation. Brewery spoilage organisms. Sensory parameters of relevance to the brewing industry offering assessments in aroma and taste evaluation. Chemical tests of relevance for alcoholic beverages and intermediate process samples. Chemical and physical tests of relevance to the malting industries for quality checks and complex analysis, including Mycotoxin analysis. Chemical tests of relevance to the beverage industries for quality checks and complex parameters. Microorganisms of relevance to beverage products, including pathogens and indicator organisms. Chemical tests of relevance to the sugar processing, natural sweeteners and food industries. Microorganisms of relevance to sugar products, including pathogens and indicator organisms **Please see current application form and scheme description for accredited and non-accredited status of test materials and analytes. [email protected] Website: 31

32 BAPS - Brewing analytes The BAPS scheme is designed to promote quality in the measurement of a range of chemical, microbiological and sensory analytes in real beer matrices. It is jointly run by LGC Standards and Campden BRI. Lager and ale test materials are designed to encompass a range of key brewing analytes that are routinely analysed in the brewing industry. Ale products with high bitterness content and / or high colour value are also available. Microbiological test materials contain organisms typically encountered in the brewing industry. There is a low level test material for membrane filtration and a high level test material for plate count (spread or pour). For sensory analysis, participants evaluate various aroma and taste characteristics of real beers and can compare their results with the Campden BRI sensory panel who provide an expert profile with the sample to enable immediate training of panels if required. The full range and availability of test materials in BAPS is determined on an annual basis and further details can be found in the BAPS application form and scheme description. Test material Lager / ale Lager Ale Lyophilised material Lager / ale Brewing liquor Effluent solution Analytes Chemical Alcohol by volume, Bitterness, Carbon dioxide, Colour at 430 nm, Gravity (apparent, original, present), Haze at 0ºC, Haze at 20ºC, Original extract, Refractive index, Sulfur dioxide, Total gas pressure. Chemical Acetaldehyde, Calcium, Chloride, Copper, Diacetyl as VDK, Dimethyl disulfide, Dimethyl sulfide, Energy value (kcal), Energy value (kj), Ethyl acetate, Ethyl hexanoate, FAN, Foam stability (HRV), Free diacetyl, Free 2,3-Pentanedione, Glucose, Hydrogen sulfide, Iron, Iso-alpha-acids, Iso-amyl acetate, Iso-butanol, Magnesium, Maltose, Maltotetraose, Maltotriose, Methanethiol, Methylthioacetate, Nitrate, n-propanol, Phosphate, Potassium, Sodium, Sulfate, Tetra-iso-alpha-acids, Total carbohydrate, Total polyphenols, TSN, Zinc, 2-Methyl butanol, 3-Methyl butanol, 2+3 Methyl butanol. Chemical Bitterness, Colour at 430 nm, Colour at 530 nm, Diacetyl as VDK, Free diacetyl, Free 2,3-Pentanedione, Iso-alpha-acids, Tetra-iso-alpha-acids. Microbiological Identity of organisms, Lactic acid bacteria, Total aerobic bacterial count (pour), Total aerobic microbial count (spread), Total anaerobic microbial count, Wild yeast. Sensory Aroma Alcoholic / Solvent, Burnt, Caramel, Cereal, DMS, Fruity / Citrus, Fruity / Estery, Hop, Malty, Other sulfur, Oxidised / Aged, Sweet, and others. Taste Alcoholic / Solvent, Astringent, Bitter, Body, Burnt, Caramel, Cereal, DMS, Fruity / Citrus, Fruity / Estery, Hop, Linger, Malty, Other sulfur, Oxidised / Aged, Sour, Sweet, and others. Calcium, Conductivity at 20ºC, Copper, Hardness (as carbonate), Iron, Magnesium, ph at 20-25ºC, Sodium, Sulfate. Non filterable COD, Suspended solids, Total COD. Note: Test materials and analytes may be added or removed, please see current application form. 32 Tel: +44 (0) Fax: +44 (0)

33 DAPS - Alcoholic drinks As well as distilled beverages, DAPS covers analysis of a wide range of alcoholic beverages and intermediate process samples. There are five different groups available, with a comprehensive range of test parameters. One of the key tests performed within DAPS is the measurement of alcoholic strength. Duty is normally paid on the basis of the alcohol in the beverage, and so it is a key performance indicator for the product and tax authorities. By monitoring laboratory performance in this key area, laboratories can help ensure that overpayment is not made thus saving money. Underpayment, which could lead to financial penalties, can also be avoided. Many of the key parameters in DAPS are related to flavour compounds within the beverage and so, by ensuring consistent analysis of these parameters, beverage producers may improve consistency of product across batches. The full range and availability of test materials in DAPS is determined on an annual basis and further details can be found in the DAPS application form and scheme description. Test material Worts Distilled spirits Ciders Wines and fortified wines Other alcoholic beverages Analytes Alcohol, Gravity (original, residual, final), ph, Residual fermentable sugars (total amount of glucose, maltose and maltotriose). Acetic acid, Actual alcoholic strength, Apparent alcoholic strength, Acetal, Acetaldehyde, Calcium, Citric acid, Colour, Copper, Ethyl acetate, Ethyl carbamate, Fructose, Furfural, Glucose, Glycerol, Haze, Iron, Iso-amyl acetate, Iso-butanol, Linalool (gin only), Magnesium, Maltose, Methanol, n-butanol, NDMA, n-propanol, ph, Potassium, Propylene glycol, Sodium, Sucrose, Terpinene-4-ol (gin only), Total acidity, Total sugars, Volatile acidity, 2-Methyl butanol, 3-Methyl butanol, Methyl butanols. Actual alcoholic strength, Carbon dioxide, Colour, Haze, ph, Specific gravity, Total acidity, Total sulfur dioxide, Volatile acidity. Actual alcoholic strength, Ascorbic acid, Citric acid, Colour at 420 nm, Colour at 520 nm, Colour at 620 nm, Copper, Free sulfur dioxide, Fructose, Glucose, Iron, Lactic acid, Malic acid, ph, Reducing sugars, Sorbic acid, Specific gravity, Total acidity, Total sulfur dioxide, Volatile acidity. Acetal, Acetaldehyde, Actual alcoholic strength, Ascorbic acid, Benzoic acid, Brix, Carbon dioxide, Citric acid, Ethyl acetate, Furfural, Glycerol, Iso amyl acetate, Iso-butanol, Methanol, n-butanol, n-propanol, ph, Propylene glycol, Residue, Sorbic acid, Specific gravity, Total acidity, Total sugars, Volatile acidity, 2-Methyl butanol, 3-Methyl butanol, Methyl butanols. Note: Test materials and analytes may be added or removed, please see current application form. [email protected] Website: 33

34 MAPS - Malt analytes The MAPS scheme covers test materials from the full range of malted barley and barley used for brewing and distilling. These test materials are analysed for a wide range of analytes, using European Brewing Convention (EBC) and Institute of Brewing and Distilling (IBD) methods, as well as a number of other physical and chemical methods. All test materials are sourced from commercially traded malting barleys. Malt is a complex product and forms a key ingredient in brewing and distilling. It is important in terms of soluble starch content, as this is converted to sugar in the mashing process, partly determining the alcohol yield during fermentation. Malt also makes a contribution to the flavour of the final product, whether it is used to produce a distilled spirit or a beer. Malt is considered the heart of the process, providing most of the sugars and complex carbohydrates which produce the alcohol and flavour. It can also contribute to the physical attributes of beer such as colour and foam. Meeting the demanding specifications laid down by brewers and distillers is critical to the business of any maltster and is greatly dependent on the quality of the malting barley. For this reason the accuracy of laboratory results is essential as they will ultimately decide if the product is suitable for use in the production plant. The full range and availability of test materials in MAPS is determined on an annual basis and further details can be found in the MAPS application form and scheme description. Test material Brewers and distillers malt Barley Malt flour Malt (Black and crystal) Crystal malt Analytes Chemical Alpha amylase, Cold water extract, Diastatic power, DMSP, DPWK, EBC fraction IV (<2.2 mm + damaged corns from all other sieves), EBC reject fraction (EBC Fraction IV plus foreign matter), Free and Total DMS, Friability, Glassy (whole) corns, Glycosidic nitrile, Hartong VZ45, Homogeneity, Malt mod homogeneity, Malt modification, Moisture, NDMA, Partly unmodified grains, Residual sulfur dioxide, Sieving test (<2.20 mm, 2.20 to 2.50 mm, 2.50 to 2.80 mm,>2.80 mm), Total nitrogen, Total phenols. EBC wort dependent Beta glucan, Boiled wort colour, colour, DMSP, Extract difference ( ), Extract: (0.2 mm, 1.0 mm), FAN, Fermentability (boiled), Kolbach index, ph, TSN, Viscosity. IoB wort dependent Beta glucan, Colour, Extract (0.2 mm, 0.7 mm), Extract difference (0.2 mm-0.7 mm), FAN, Fermentability (Boiled, Unboiled 2.0 mm, 0.7 mm), ph, Predicted spirit yield (as is), SNR, Soluble extract difference ( ), ( ), Soluble extract 0.2 mm, 0.7 mm, 1.0 mm, TSN, Viscosity. Chemical BRF (8ml test), EBC fraction IV (<2.2 mm + damaged corns from all other sieves), EBC reject fraction (EBC fraction IV plus foreign matter), Germinative capacity, Germinative energy, Hectolitre weight, Moisture, Sieving test <2.20 mm, <2.25 mm, 2.20 to 2.50 mm, 2.25 to 2.50 mm, >2.50 mm, 2.50 to 2.80 mm, >2.80 mm, Thousand corn weight, Total nitrogen. Mycotoxin Deoxynivalenol (DON), Ochratoxin A. Nitrosodimethylamine (NDMA). Chemical Colour, Moisture. Chemical Degrees of crystallisation. Note: Test materials and analytes may be added or removed, please see current application form. 34 Tel: +44 (0) Fax: +44 (0)

35 QBS - Soft drinks and fruit juices The Quality in Beverages Scheme (QBS) is specifically tailored for chemists and microbiologists working in the fruit juice, and soft drinks industries (including carbonated drinks). The scheme can also be of value to other laboratories that need to test for general food additives, such as sweeteners and preservatives. Fruit juice and beverage products are regularly tested to ensure that they are of acceptable chemical and microbiological quality for consumption. Chemical test materials are ready-to-use liquid matrices, covering product quality and compositional values. This enables laboratories to assess performance and demonstrate compliance with the relevant compositional standards and the statutory limits governing the use of additives. It also shows that the tests used to monitor other ingredients are reliable, which is essential to maintain the organoleptic properties of the beverage so vital to product quality. Test materials for microbiological analyses cover the main spoilage and indicator organisms and are provided as lyophilised material with a choice of diluent (soft drink or fruit juice) and a test material for analysis using filtration method to meet the laboratory s particular requirements. The full range and availability of test materials in QBS is determined on an annual basis and further details can be found in the QBS application form and scheme description. Test material Apple juice Carbonated drinks Carbonated drink (degassed) Dilutable and ready to drink Fruit juice Liquid test material Microbiological quality filtration Soft drink Analytes Chemical Patulin. Chemical Acidity (as Citric acid monohydrate), Brix, Carbon dioxide, Fructose, Glucose, Headspace air, Sucrose, ph. Chemical Acesulfame K, Aspartame, Benzoic acid, Caffeine, Free and total sulfur dioxide, Saccharin (as free imide), Sorbic acid (as free acid), Sucralose. Chemical Acesulfame K, Acidity (as Citric acid monohydrate), Aspartame, Benzoic acid, Brix, Caffeine, Colour, Free and total sulfur dioxide, Fructose, Glucose, Saccharin (as free imide), Sorbic acid (as free acid), Sucrose, ph. Chemical Acidity (as Citric acid monohydrate), Brix, Fructose, Glucose, ph. Heavy metals and minerals Antimony, Cadmium, Calcium, Iron, Lead, Magnesium, Phosphorus, Potassium, Sodium, Tin, Zinc. Microbiological Enumeration Lactic acid bacteria, Mould, Total aerobic mesophilic count, Yeast. Detection Escherichia coli. Chemical Ascorbic acid (Vitamin C). Microbiological Enumeration Lactic acid bacteria, Mould, Total aerobic mesophilic count, Yeast. Detection Escherichia coli. Heavy metals and minerals Antimony, Cadmium, Calcium, Iron, Lead, Magnesium, Phosphorus, Potassium, Sodium, Tin, Zinc. Vitamins Vitamin B3 (Nicotinamide), Vitamin B5 (Pantothenic acid), Vitamin B6 (Pyridoxine), Vitamin B12 (Cyanocobalamin), Vitamin C (Ascorbic acid). Microbiological Enumeration Lactic acid bacteria, Mould, Total aerobic mesophilic count, Yeast. Detection Escherichia coli. Note: Test materials and analytes may be added or removed, please see current application form. [email protected] Website: 35

36 SUPS - Sugar scheme SUPS is designed to provide sugar and other natural sweeteners analysts with information on technical issues and methodologies related to the testing of these materials. LGC Standards cooperates with the International Commission for Uniform Methods of Sugar Analysis (ICUMSA) and the International Stevia Council (ISC). The purpose of these organisations is to provide robust, internationally developed methods of analysis to aid the trade in sugar and sugar products, and Stevia and Stevia products, respectively. The ICUMSA and ISC representatives are involved in the review of progress and performance of the scheme and provide advice on operation and future development of the scheme. Participants will receive test materials quarterly for monthly analysis and reporting of results. SUPS provides data which helps with identification of any performance issues with respect to the methods used and provides evidence of competence for individual laboratories. The full range and availability of test materials in SUPS is determined on an annual basis and further details can be found in the SUPS application form and scheme description. Test material Cane or beet sugar Molasses Raw sugar Stevia Lyophilised material Analytes Chemical Ash, Colour, Polarisation, Reducing sugars, Reflectance grade, Sulfur dioxide, Turbidity. Metals Arsenic, Copper, Iron, Lead. Sediment Sediment (insoluble). Chemical Dry substance, ph, Reducing sugars, Sucrose. Chemical Ash, Colour, Dextran, Moisture, Polarisation, Reducing sugars, Starch. Chemical Dulcoside A, Rebaudioside A, Rebaudioside B, Rebaudioside C, Rebaudioside D, Rebaudioside E, Rebaudioside F, Rubusoside, Steviolbioside, Stevioside, Total steviol glycosides. Microbiological Enumeration Thermophilic acidophilic bacteria, Total aerobic mesophilic count, Osmophilic yeast and mould, Yeast and mould. Detection Guaiacol producing thermophilic acidophilic bacteria. Note: Test materials and analytes may be added or removed, please see current application form. 36 Tel: +44 (0) Fax: +44 (0)

37 Clinical PT schemes DAH Drugs of abuse in hair DAU Drugs of abuse in urine DOF Drugs in oral fluid TDM Therapeutic drugs monitoring TOX Toxicology

38 Clinical PT scheme selector Scheme Scheme year Number of distributions per scheme year *DAH Drugs of abuse in hair DAU Drugs of abuse in urine DOF Drugs in oral fluid TDM Therapeutic drugs monitoring TOX Toxicology Tests April - March Four Clinical April - March Four Clinical April - March Four Clinical January - December Twelve Clinical April - March Twelve Clinical *Please note that the DAH scheme is currently not included in our scope of accreditation. My results have not been included in the report can I calculate my z / z performance score? To calculate your performance score please visit: Select help from the menu and download the z / z performance score calculator Tel: +44 (0) Fax: +44 (0)

39 Test material matrix** Analyte group** See page Human hair. Mixtures of drugs and / or their metabolites from six major classes. 40 Urine from volunteers and known drug users. Mixtures of drugs and / or their metabolites from six major classes. 41 Oral fluid from volunteers and known drug users. Mixtures of drugs and / or their metabolites from six major classes. Blood, plasma, serum and urine. Routine quantification of therapeutic drugs Blood, serum and urine. Drug and ethanol determination. 44 **Please see current application form and scheme description for accredited and non-accredited status of test materials and analytes. [email protected] Website: 39

40 DAH - Drugs of abuse in hair The DAH scheme is suitable for all laboratories involved in the analysis of hair for drugs of abuse and provides an independent assessment of measurement quality. Drugs and their metabolites become incorporated in hair when ingested. Therefore, analysis for these drug residues can provide a useful and accurate assessment of an individual s intake of drugs over a period of time. The detection time of drugs in hair is significantly greater than other samples i.e. blood, urine and saliva. Advantages of analysing hair samples for the presence of drugs include a large window of detection, the assessment of the regularity of drug use and sample stability. Test materials provided consist of real cut (2-3 mm pieces) human hair that has been declared free from common drugs of abuse. The analytes are then incorporated by a method that includes soaking. Drug (and / or metabolites) from six major classes are included during the scheme year. Test material Human hair segments Analytes Amfetamines and stimulants, Benzodiazepine, Cannabinoids, Cocaine and metabolites, Opiates. Note: Test materials and analytes may be added or removed, please see current application form. DAH is currently not included in our scope of accreditation. The full range and availability of test materials in DAH is determined on an annual basis and further details can be found in the DAH application form and DAH scheme description. 40 Tel: +44 (0) Fax: +44 (0)

41 DAU - Drugs of abuse in urine The DAU scheme is designed to provide an independent performance assessment of laboratories and clinics that provide routine services for detection of drugs of abuse in urine. Human urine has been used for many years to detect the presence of frequently abused drugs. A urine drug test may be undertaken when ordered by a doctor to monitor a known or suspected substance abuse patient, and whenever a person has symptoms that suggest drug use. Urine drug tests may be requested for a variety of reasons, including occupational monitoring, insurance screening, legal and forensic purposes and in sports. Drug testing is extremely accurate and reliable when all aspects of the testing process are carried out correctly. However, if poor procedures and inadequate testing methods are utilised, the information obtained may be very misleading and inaccurate. In order to minimise this risk, clinical laboratories should perform routine quality control tests and participate in suitable PT / EQA schemes. Test material Urine test materials obtained from volunteers, patients and known drug users which regularly contain mixtures of drugs and their metabolites from six major classes. Analytes Amfetamines and stimulants, Cannabinoids, Cocaine and metabolites, Minor tranquillizers, Non-opiate narcotics, Opiates. Note: Test materials and analytes may be added or removed, please see current application form. The full range and availability of test materials in DAU is determined on an annual basis and further details can be found in the DAU application form and scheme description. [email protected] Website: 41

42 DOF - Drugs in oral fluid The DOF scheme is designed to provide an independent performance assessment of laboratories and clinics that provide analytical services for drugs in oral fluid. Drug abuse is a global problem affecting many in society. Advances in technology have enabled oral fluid testing for the presence of many drugs. Oral fluid as a testing matrix is increasingly being utilised in a range of applications, such a work place monitoring, clinical toxicology and criminal justice. Test material Oral fluid test materials obtained from volunteers and known drug users which regularly contain mixtures of drugs and their metabolites from six major classes. Analytes Amfetamines and stimulants, Cannabinoids, Cocaine and metabolites, Minor tranquillizers, Non-opiate narcotics, Opiates. The advantages of oral fluid over traditional fluids such as blood and urine, are that collection is almost non-invasive, is relatively easy to perform, and, in forensic situations, can be achieved under close supervision to prevent adulteration or substitution of the samples. Note: Test materials and analytes may be added or removed, please see current application form. Due to the importance of results obtained it is essential that laboratories undertaking the analyses are able to demonstrate the testing they perform is dependable, reproducible and accurate. Participation in the DOF scheme will provide valuable feedback to laboratories / on-site screening clinics who undertake these analyses, and a record of results overtime. The full range and availability of test materials in DOF is determined on an annual basis and further details can be found in the DOF application form and scheme description. 42 Tel: +44 (0) Fax: +44 (0)

43 TDM - Therapeutic drugs monitoring The TDM scheme is designed to provide an independent performance assessment for laboratories and clinics who are involved in the routine quantification of therapeutic drugs in serum. TDM is the measurement of specific drug levels (concentrations) at timed intervals in patients, usually through blood samples, and is necessary where control of drug concentrations is required to achieve optimum treatment for the patient. For most drugs, monitoring is not required as they have a wide therapeutic index i.e. the difference between a therapeutic and toxic concentration is large. Therefore, most individuals will be effectively treated without extreme side effects or symptoms of toxicity. However, drugs with a narrow therapeutic range may result in a high or low serum concentration if not monitored and controlled. Drug concentrations in the bloodstream that are too high have the potential to exert adverse effects associated with toxic or even fatal consequences. Drug concentrations below the therapeutic range can lead to poor response to treatment. For some drugs, maintaining this steady state is not as simple as giving a standard dose of medication. The full range and availability of test materials in TDM is determined on an annual basis and further details can be found in the TDM application form and scheme description. Note: Test materials and analytes may be added or removed, please see current application form. *These analytes are produced in collaboration with the UKNEQAS scheme for antibiotics drugs. ** This scheme is managed and operated by ASI Ltd. and is not included in LGC Standards UKAS scope of accreditation. The scheme complements the LGC TDM scheme and participants may order the scheme through LGC Standards. Test material Anti-epileptic and other therapeutic drugs Human serum. Anti-epileptic drugs mixture Human serum. Anti-epileptic drugs mixture 1 Human serum. Anti-epileptic drugs mixture 2 Human serum. Anti-epileptic drugs mixture 3 Human serum. Anti-epileptic drugs mixture 4 Human serum. Cardiac drugs mixture Human serum. Drugs for the treatment of substance related disorders 1 Human serum. Drugs for the treatment of substance related disorders 2 Human serum. Psychoactive drugs Newborn calf serum. Psychoactive drugs Human serum. Smoking related drugs Human urine. *Antibiotic drugs Human serum. **Immunosuppressive drugs Human blood / plasma. Analytes Caffeine, Carbamazepine + CBZ-epoxide, Carbamazepine, CBZ-epoxide, Clonazepam, Digoxin, Ethosuximide, Lamotrigine, Lithium, Methotrexate, Phenobarbitone, Phenytoin, Primidone, TD-Amikacin, TD-Gentamicin, TD-Tobramycin, TD-Vancomycin, Theophylline, Valproate. Clobazam, Norclobazam. Gabapentin, Levetiracetam, OH-oxcarbazepine, Pregablin, Tiagabine. Felbamate, Lacosamide, Rufinamide, Topiramate, Vigabatrin, Zonisamide. Retigabine (Ezogabine). Perampanel. Amiodarone, Desethylamiodarone, Flecainide. Buprenorphine, Norbuprenorphine. EDDP, Methadone. Amisulpride, Amitriptyline / Nortriptyline, Aripiprazole / Dehydroaripiprazole, Citalopram / Norcitalopram, Clomipramine / Norclomipramine, Dothiepin / Northiaden, Doxepin / Nordoxepin, Duloxetine, Escitalopram, Fluoxetine / Norfluoxetine, Fluphenazine, Fluvoxamine, Haloperidol, Imipramine / Desipramine, Maprotiline / Normaprotiline, Mirtazapine / Normirtazapine, Paroxetine, Perphenazine, Quetiapine and Norquetiapine, Risperidone / HO-risperidone, Sertraline / Norsertraline, Thioridazine, Trimipramine / Nortrimipramine, Trazodone, Venlafaxine / Norvenlafaxine, Ziprasidone, Zuclopenthixol. Clozapine / Norclozapine, Olanzapine. Nicotine and Cotinine. Amikacin, Chloramphenicol, Flucytosine, Gentamicin and vancomycin, Netilmicin, Teicoplanin, Tobramycin. Cyclosporin, Everolimus, Mycophenolate, Sirolimus, Tacrolimus. [email protected] Website: 43

44 TOX - Toxicology The TOX scheme is designed to provide an independent performance assessment for laboratories and clinics that provide a pathology service, toxicological service, or forensic investigation service for drug and ethanol determination. Drug and alcohol analyses may be undertaken for a variety of reasons which include but are not restricted to: Determine whether an individual is under the influence of alcohol and / or drugs that may interfere with any medical care necessary (These analyses are usually required quickly in order to facilitate effective medical treatment) Determine whether an individual is under the influence of alcohol and / or drugs which may put their own life or that of others at risk whilst at work Determine whether an individual may have been affected by alcohol and / or drugs whilst either a victim or a suspect in a criminal offence Determine whether alcohol and / or drugs may be implicated in a death Test material Human serum Human blood Urine Lyophilised urine Toxic alcohols in blood Human serum and urine Whole blood toxicology Analytes Ethanol, Paracetamol, Salicylic acid. Carboxyhaemoglobin, Ethanol, Paracetamol, Salicylic acid. Ethanol. Gammahydroxybutyrate (GHB). Acetone, Ethanol, Ethylene glycol, Isopropyl alcohol (IPA), Methanol. Toxicology case studies include various analytes with clinical or forensic scenario. Samples contain analytes which are pre-defined by our Advisory Group and the request of participants. Toxicological analyses may be undertaken on a number of biological specimens, predominantly blood, serum and urine. In general, analyses are commonly undertaken for a range of prescription and non-prescription drugs, illegal drugs and alcohol. The aim of the analyses is to establish the identity of any substances present and at what quantity, and to then determine what effect the substance(s) identified may have had on the individual. The analytical findings would then be subject to interpretation by a suitably qualified individual. Note: Test materials and analytes may be added or removed, please see current application form. The full range and availability of test materials in TOX is determined on an annual basis and further details can be found in the TOX application form and scheme description. 44 Tel: +44 (0) Fax: +44 (0)

45 Consumer safety PT schemes COSMETICS Cosmetics and toiletries NiMS Nickel migration PHARMASSURE Pharmaceutical TOYTEST Toy safety

46 Consumer safety PT scheme selector Scheme Scheme year Number of distributions per scheme year COSMETICS Cosmetics and toiletries NiMS Nickel migration PHARMASSURE Pharmaceutical TOYTEST Toy safety Tests April - March Four Chemical and microbiological January - December Two Chemical and physical April - March Four Chemical, microbiological, physical and instrument techniques January - December Four Chemical assessment against standards and paper exercise How can I receive advice and feedback? Communication with participants will be carried out through scheme-related documentation, s, letters or through LGC Standards regional offices. Open meetings may also be organised and all interested parties invited to attend Tel: +44 (0) Fax: +44 (0)

47 Test material matrix** Analyte group** See page Cosmetics and toiletries. Alloy disks, jewellery or other appropriate articles. Pharmaceutical products and standard solutions. Chemical parameters of relevance to the cosmetics 48 and toiletries testing industries. Microbiological tests including spoilage and indicator organisms. Nickel release and surface area. 49 Basic and advanced chemical analysis, microbiological analysis and sterility testing. 50 Toys, standard solutions and paper exercises. Metals analysis, toy safety assessment exercises and physical measurement assessments. 51 **Please see current application form and scheme description for accredited and non-accredited status of test materials and analytes. Website: 47

48 COSMETICS - Cosmetics and toiletries The use of cosmetics has been commonplace for thousands of years, and the potential for harm to the wearer has been present ever since the use of toxic chemicals such as lead sulfite (galena) as an eye make-up by the ancient Egyptians. Today it is important that contaminated cosmetics do not enter the supply chain, whether the contamination is due to excessive levels of heavy metals, or microbial contamination which can produce enzymes which can damage the epidermis causing irritation and potentially infection if applied to already broken skin. The COSMETICS scheme covers chemistry and microbiology test parameters. A lipstick, lip gloss and powdered cosmetic formulation are available for analysis of lead and other metals. In addition to this, a cream test material is available for the analysis of hydroquinone. This analyte is commonly found in products marketed to lighten the skin, and is one of the most toxic ingredients still used in cosmetics. For the analysis of common microbiology contaminants, the range of products includes cream, liquid and powder. The full range and availability of test materials in COSMETICS is determined on an annual basis and further details can be found in the COSMETICS application form and scheme description. Test material Lipstick, lip gloss and powder Cream Liquid Cream, liquid and powder Analytes Trace elements Cadmium, Chromium, Lead, Nickel. Chemical Hydroquinone. Physicochemical ph, Specific gravity, Viscosity. Microbiological Enumeration Aerobic mesophilic bacteria, Enterobacteriaceae, Pseudomonas aeruginosa, Staphylococcus aureus, Yeast and mould (total count). Detection Aerobic mesophilic bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Note: Test materials and analytes may be added or removed, please see current application form. 48 Tel: +44 (0) Fax: +44 (0)

49 NiMS - Nickel migration The NiMS scheme is designed to assess the performance of laboratories undertaking the determination of nickel release from articles intended to come into direct and prolonged contact with the skin according to the European Regulation (EC) 1907/2006. Test material Alloy disks, jewellery or other appropriate articles Analytes Chemical Nickel release and surface area measurement. Note: Test materials and analytes may be added or removed, please see current application form. The release of nickel from certain products, most commonly earrings and other jewellery for body piercings is the most widespread cause of allergic contact dermatitis - an itchy rash that appears when skin touches a usually harmless substance - affecting people of all ages. Many ordinary items such as buttons, coins, spectacle frames, watch straps, zips etc. may also contain nickel and as such is hard for sufferers to avoid. The method for the determination of nickel release from such products is defined in European standard EN 1811 (2011). The article to be tested is suspended in an artificial sweat solution for a period of a week, after which time the concentration of dissolved nickel in the solution is determined by ICP-MS or a similarly accurate and precise technique. The full range and availability of test materials in NiMS is determined on an annual basis and further details can be found in the NiMS application form and scheme description. [email protected] Website: 49

50 PHARMASSURE - Pharmaceutical The regulations surrounding the manufacture of pharmaceutical products and the laboratories which test them are wide ranging, and compliance with the requirements of GMP/GLP is aimed at ensuring the safety and efficacy of all pharmaceutical preparations. Similarly, the annual revalidation of methods is an important tool in understanding and ensuring that a testing method is working properly. However, these tools are largely internal operations. Regular participation in a proficiency testing scheme, provides an external independent tool for monitoring the effectiveness of methods employed in any laboratory, without the high level of work involved in a full method revalidation. PHARMASSURE has been specifically designed to meet the needs of the pharmaceutical industry. The reduction of risks associated with the manufacture of pharmaceutical products is the primary objective of the pharmaceutical quality assurance laboratory. There is an abundance of regulations, biological standards, validation procedures and other tools available to the quality control professional to achieve this aim. In most cases however, the use of these tools is aimed at assessing the product or the environment in which it was produced. The introduction of proficiency testing offers a valuable, independent, tool for the ongoing assessment of the testing procedures, methods employed, laboratory staff responsible for the analysis and subsequent interpretation of results. Test material Varying volumes in diverse formats Sample format will depend upon test type Format varies from round to round Powder material Tablet Lyophilised test material (low level microorganism) Lyophilised test material (high level microorganism) Lyophilised test material Analytes Basic chemical analysis Acid and Base titration, Density, Melting point, Other basic titration (actual analytes dependent on test), ph, Refractive index. Advanced chemical analysis Advanced titration (e.g. Potentiometric, non-aqueous), Flame spectroscopy, GC, IR/FTIR, Loss on drying, Moisture by Karl Fischer, Polarimetry, TLC, UV, Viscosity. HPLC analysis. Trace elements Arsenic, Cadmium, Lead, Mercury. Dissolution. Physical measurement Diameter, Disintegration, Friability, Hardness, Resistance to crushing, Thickness, Weight, etc. Low level enumeration and identification of microorganism. Microbiological Enumeration Candida albicans, Escherichia coli, Staphylococcus aureus, Total aerobic mesophilic count, Total bacterial count, Mould, Yeast, Total yeast and mould. Detection Candida albicans, Escherichia coli, Staphylococcus aureus. Sterility testing Identification of microorganism, Sterility. Note: Test materials and analytes may be added or removed, please see current application form. The test materials cover chemical, microbiological and physical analysis as well as sterility testing. The full range and availability of test materials in PHARMASSURE is determined on an annual basis and further details can be found in the PHARMASSURE application form and scheme description. 50 Tel: +44 (0) Fax: +44 (0)

51 TOYTEST - Toy safety Play is a vital part of child-development and toys help at every stage from babies to school-age children. Toys are safer than ever before, but we should remain cautious about often hidden hazards of toys. We rely on manufacturers, suppliers and toy legislation to help keep our children safe, and at the same time prevent product recalls and protect brand value. The TOYTEST scheme allows laboratories involved in the safety testing of toys to monitor their analysis against the current European and American standards. Test materials are assessed against the requirements of European Standard EN71 and American Society for Testing and Materials (ASTM) F963 toy safety standards. A comprehensive range of test materials and product information will be included over the scheme year to be assessed against the regulations. Test materials will also be provided for analytical testing, such as paint flakes for cadmium and lead analysis; standard solutions for heavy metal analysis; plastic for analysis of phthalates and other materials for a range of analyses. The full range and availability of test materials in TOYTEST is determined on an annual basis and further details can be found in the TOYTEST application form and scheme description. Test material Toy product for paper exercise Toy product for paper exercise Section of material or toy product Real material and standard solution Information and / or toy product for paper exercise Information and / or toy product for paper exercise Information and / or toy product for paper exercise Dried paint flakes Section of material Section of plastic material and test solutions Magnetic material Selection of products Toy product Toy product Analytes EN71-1 Mechanical and physical testing. ASTM F963 Mechanical and physical testing. EN71-2 Flammability testing. EN71-3 Aluminium, Antimony, Arsenic, Barium, Boron, Cadmium, Chromium, Chromium III, Chromium VI, Cobalt, Copper, Lead, Manganese, Mercury, Nickel, Organic Tin, Selenium, Strontium, Tin, Zinc. EN71-7 Finger paint. EN71-8 Outdoor activity toys. EN71-9 Organic chemical compounds. Total cadmium, Total lead. Azo dyes. Phthalates. Flux testing. Measurement testing. Acoustic testing. Kinetic energy testing. Note: Test materials and analytes may be added or removed, please see current application form. Please note, not all parts of the standards will be assessed over a scheme year. Participants will be given clear instructions as to which parts of the standards are to be assessed. [email protected] Website: 51

52 NEW PRODUCT RANGE Can t find an official pharmacopoeial standard? Try our new range of pharmaceutical primary standards! The FDA Guidance for Industry on Analytical Procedures and Methods Validation states: When there is no official source, a reference standard should be of the highest possible purity and be fully characterised. LGC Standards has launched a new range of pharmaceutical primary reference standards that fulfill FDA s and other regulation bodies requirements. Please see our range of primary reference standards available from stock under We also provide primary standards on a customised basis. The certificate of your primary standard from LGC includes: Detailed identity information: Nuclear magnetic resonance (NMR) spectrum Infrared spectrum (IR) Mass spectrometry data (MS) Detailed purity/assay data: Assay information by accredited technique, qnmr whenever possible Confirmation by orthogonal method, e.g. mass balance Further information at LGC Quality - ISO Guide 34 GMP/GLP ISO 9001 ISO/IEC ISO/IEC LGC Limited, All rights reserved. LGC Standards is part of the LGC Group. 3664/LB/0713

53 Forensics PT schemes FAE Forensic analysis for explosives FIRMS Forensic isotope ratio mass spectrometry QUARTZ Forensic blood toxicology

54 Forensics PT scheme selector Scheme Scheme year Number of distributions per scheme year FAE Forensic analysis for explosives *FIRMS Forensic isotope ratio mass spectrometry QUARTZ Forensic blood toxicology Tests April - March One Chemical January - December Two Chemical April - March Four Toxicology and case study *Please note that the FIRMS scheme is currently not included in our scope of accreditation. What could be the cause of my poor performance? A single poor result is not indicative of overall laboratory performance but neither is a single good result. Ideally, PT results should be monitored over time to detect potential bias or repeated unsatisfactory results. There are numerous potential causes of poor performance in a PT scheme which may include analytical and non-analytical errors Tel: +44 (0) Fax: +44 (0)

55 Test material matrix** Analyte group** See page Range of materials consistent with a potential crime scene. Explosive, trace explosives and unknowns. 56 Range of products in sealed amber vials. Variations in isotope ratios. 57 Blood. Alcohol and drugs analysis. 58 **Please see current application form and scheme description for accredited and non-accredited status of test materials and analytes. [email protected] Website: 55

56 FAE - Forensic analysis for explosives The FAE scheme is designed to enable laboratories providing these services to monitor their performance and compare it with that of their peers. The European Network of Forensic Science Institute (ENFSI) Working Group on Explosives provides technical advice to LGC Standards on the organisation of this scheme. Explosives analysis may be performed in a number of circumstances. Examples of these include the analysis of raw materials found at a scene in what may be a bomb making laboratory (scene), the identification of what may be explosive substances and the forensic identification of post-blast explosive residues. Test material Range of materials consistent with a potential crime scene Analytes Explosive, trace explosives and unknowns. Note: Test materials and analytes may be added or removed, please see current application form. The full range and availability of test materials in FAE is determined on an annual basis and further details can be found in the FAE application form and scheme description. 56 Tel: +44 (0) Fax: +44 (0)

57 FIRMS - Forensic isotope ratio mass spectrometry Isotope Ratio Mass Spectrometry (IRMS) is a specialised technique used to precisely measure small differences in the abundances of isotopes such as 2H/1H, 13C/12C, 15N/14N and 18O/16O. Subtle variations to the natural abundance of these isotopes may be introduced during biological, chemical and physical processes As a result, isotopic variations are found in diverse materials and IRMS therefore has a wide range of applications, in subject areas such as Archaeology, Geosciences, Biological sciences and Forensic sciences. Over recent years there has been a surge in IRMS application most notably in forensic science where it may be possible to distinguish samples which otherwise share identical chemical compositions. A laboratory that has little experience with IRMS may obtain results that appear accurate but do not reflect the samples that were analysed. Test material Range of products in sealed amber vial Analytes Variations in isotope ratios. Note: Test materials and analytes may be added or removed, please see current application form. FIRMS is currently not included in our scope of accreditation. The Forensic Isotope Ratio Mass Spectrometry (FIRMS) Network was founded to develop the scope of stable isotope techniques in forensic applications. The purpose of the scheme is to raise awareness of the relevance and importance of IRMS in forensic science, crime detection and reduction. The FIRMS scheme is run by LGC Standards and is supported by the FIRMS Network which provides input for the choice of test materials. The full range and availability of test materials in FIRMS is determined on an annual basis and further details can be found in the FIRMS application form and scheme description. [email protected] Website: 57

58 QUARTZ - Forensic blood toxicology QUARTZ is a blood toxicology scheme and is designed for laboratories undertaking analysis of drugs in post-mortem and other blood samples for toxicological purposes, particularly in a forensic context. Case-types that are covered by the scheme include deaths, drug facilitated sexual assaults, impaired driving and other forensic toxicology criminal cases. Test materials are prepared using pre-screened human blood. Target analytes are added at an appropriate level and the test material mixed to ensure homogeneity prior to despatch. Drugs and metabolites are included in the scheme that are particularly relevant to forensic toxicology and are discussed regularly with the Advisory Group. The scheme includes various samples containing forensically relevant drugs, in both known quantitative samples and unknown samples involving case scenarios. Alcohol is a major cause of road casualties and deaths and as penalties for drink-driving are severe, it is essential the accuracy of analysis can be proven in the legal case. Therefore the scheme contains a sample specifically aimed at laboratories who undertake blood alcohol determinations for Driving Offences. In addition, it may be necessary to undertake a calculation commonly known as an Alcohol Technical Defence (ATD) calculation when there has been a delay between the incident and the evidential sample being obtained and alleged post-incident alcohol consumption during this time period. The scheme includes a case scenario which can be used to demonstrate competence in carrying out the (ATD) calculation. The full range and availability of test materials in QUARTZ is determined on an annual basis and further details can be found in the QUARTZ application form and scheme description. Test material Forensic toxicology drugs Blood. Forensic toxicology drugs Blood and case study. Abuse and prescribed drugs Blood. New psychoactive substances (NPS) Blood. Alcohol in blood Blood. Alcohol technical defence Paper exercise. Analytes Identification Anaesthetics, Anticholinergics, Anticonvulsants, Antidepressants, Antihistamines, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoids, Carboxyhaemoglobin, Cardiovascular drugs, Erectile dysfunction, Hypnotic drugs, Non steroidal anti-inflammatory analgesics, Opioid analgesics, Stimulants. Quantification Anaesthetics, Anticholinergics, Anticonvulsants, Antidepressants, Antihistamines, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoids, Carboxyhaemoglobin, Cardiovascular drugs, Erectile dysfunction, Hypnotic drugs, Non steroidal anti-inflammatory analgesics, Opioid analgesics, Stimulants. Quantification Drugs of abuse Amphetamine, Diazepam, Methadone, Morphine. Prescribed drugs Amitriptyline, Citalopram, Codeine, Tramadol. Quantification 4-Methylethcathinone, Flubromazepam, MDPV, Mephedrone, Methiopropamine, Methoxetamine, PMMA. Quantification Ethanol, Fluoride. Blood and breath alcohol concentrations. Note: Test materials and analytes may be added or removed, please see current application form. 58 Tel: +44 (0) Fax: +44 (0)

59 Other PT schemes OIL PT Oils and fuels QMIS Microbiology investigation competency

60 Other PT scheme selector Scheme Scheme year Number of distributions per scheme year Tests *OIL PT Oils and fuels QMIS Microbiology investigation competency January - December Twelve Chemical and physical January - December Two Microbiological *Please note that the OIL PT scheme is currently not included in our scope of accreditation. Can I suggest a scheme or test material? We welcome suggestions any time. Please complete the Wish list form on our website Tel: +44 (0) Fax: +44 (0)

61 Test material matrix** Analyte group** See page Oils, fuels and lubricants. Various chemical and physical. 62 Lyophilised material for basic identification and Salmonella identification by serology. Photograph and paper exercise for colony identification and counting techniques. Biochemical, microscopical serology, identification and counting. 63 **Please see current application form and scheme description for accredited and non-accredited status of test materials and analytes. Website: 61

62 OIL PT - Oils and fuels Petroleum products, such as oils and fuels, are tested throughout their life span, from the time the oil is taken from the ground to beyond the petroleum recyclers. The concentration of contaminants and trace elements is vital in ensuring the quality of oil and petroleum products. Regulations related to cleanliness standards in engines; contamination particles and water in diesel fuel; instability of ultra low sulfur fuels; and many more, mean it is essential that laboratories performing these measurements are capable of producing accurate data. The OIL PT scheme is designed specifically to assist chemists and engineers working in the refinery, fuel, used oil and lubricant laboratories to meet these regulations. Laboratories performing the analysis of fuels and oils according to ASTM, IP and ISO protocols will be able to monitor their performance and compare it with that of their peers and other laboratories worldwide. The full range and availability of test materials in OIL PT is determined on an annual basis and further details can be found in the OIL PT application form and scheme description. Test material Analytes Ultra low sulfur diesel fuel Sulfur content. #2 Diesel fuel Acid and base number, Ash, BP distribution, Carbon residue, Cloud point, Cold filter plugging point, Colour, Copper corrosion, Density, Distillation, Elements (carbon, hydrogen, nitrogen), Flash point, Heat content, High temperature stability, Hydrocarbon type(s), Lubricity (HFRR) wear scar diameter at 25 ºC, Particulate contamination by filtration, Pour point, Sulfur content, Viscosity (kinematic), Water and sediment. Aviation turbine fuel (Jet A) Crude oil Acidity, BOCLE lubricity, BP distribution, Colour, Combustion (net heat), Copper corrosion, Density, Distillation, Flash point, Freeze point, Gum, Hydrocarbon type, Hydrogen content, Mercaptan sulfur, Naphthalenes, Smoke point, Sulfur content, Thermal stability, Viscosity (kinematic), Water separation. Asphaltenes, Density (relative density or API gravity), High temperature simulated distillation (HTSD), Metals (vanadium, nickel, iron), Micro carbon residue, Pour point, Reid vapour pressure, Salt, Sediment, Sulfur, Total acid number, Total nitrogen, Viscosity (kinematic), Water. Engine oil lubricants Acid number, Additive elements (barium, calcium, magnesium, molybdenum, phosphorous, potassium, silicon, sodium, zinc), Ash, Ash sulfated, Base number, Colour, Density, Evaporating loss, Flash point, Nitrogen, Pour point, Saponification number, Shear stability, Sulfur content, Viscosity (HTHS, kinematic, low temperature, tapered bearing, tapered plug), Volatility, Water, Water content. Note: Test materials and analytes may be added or removed, please see current application form. OIL PT is currently not included in our scope of accreditation. 62 Tel: +44 (0) Fax: +44 (0)

63 QMIS - Microbiology investigation competency An area of increasing importance for analysts is being able to demonstrate competency in carrying out fundamental microbiological techniques. QMIS may help with this as it is designed to work alongside all the schemes that require microbiological analysis. QMIS differs from the normal schemes in that the microbiologist must carry out laboratory techniques and apply further investigative measures in order to identify an unknown single organism. Each test is assessed in the report, as is the overall identification. There is a sample specifically for serology testing, to identify a strain of Salmonella, and a case study for enumeration of a typical culture plate. Participants will be given a photograph of a real culture plate with the associated dilution factor. They must calculate the level of the stated organism in the original sample. This is seen as a straight forward procedure, but in our experience interpretation of colonies, enumeration and calculation can lead to significant errors. Test material Unknown single lyophilised microorganism Lyophilised Salmonella strain Photograph and paper exercise Analytes Catalase, Gram stain, Identification, Indole test at 37 C, Motility, OF (oxidation-fermentation), ONPG, Oxidase, Presence of spores, Shape, Urea and other routine tests. Salmonella serology. Colony identification, Counting techniques. Note: Test materials and analytes may be added or removed, please see current application form. The full range and availability of test materials in QMIS is determined on an annual basis and further details can be found in the QMIS application form and scheme description. [email protected] Website: 63

64 O O O OH O N N N OH OH O HCl OH Pharmaceutical impurities LGC Standards Pharmaceutical impurities and primary reference standards 2014 Code Product Adiphenine Hydrochloride Adrenaline Tartrate CAS No. CS Price Unit MM Adiphenine Hydrochloride A 250mg HCl MM Adrenaline Tartrate A 500mg O MM L-Adrenaline A 500mg MM Imp. C (EP) as Hydrochloride: 1-(3,4-Dihydroxyphenyl)-2-(methylamino)ethanone Hydrochloride (Adrenalone Hydrochloride) OH N H OH OH MM (1R)-1-(3,4-Dihydroxyphenyl)-2- methylaminoethanesulphonic Acid (Adrenaline -Sulphonate) OH OH OH H N A 100mg HCl Alanine OH OH O N H A 100mg OH O S O H OH N MM Alanine A 500mg OH MM Imp. A (Pharmeuropa): (2S)-2-Aminobutanedioic Acid (Aspartic Acid) NH 2 OH Albendazole A 500mg OH O MM Albendazole A 500mg O NH 2 OH MM O MM Imp. A (EP): 5-(Propylsulphanyl)-1Hbenzimidazol-2-amine MM Imp. B (EP): Methyl [5-Propylsulphinyl)- 1H-benzimidazol-2-yl]carbamate A 100mg S H N N O N H O H N MM S N NH2 MM Imp. C (EP): Methyl [5-Propylsulphonyl)- 1H-benzimidazol-2-yl]carbamate Imp. D (EP): 5-(Propylsulphonyl) -1Hbenzimidazol-2-amine A 100mg S O O H N O N N H A 100mg S Alfuzosin Hydrochloride Imp. E (EP): Methyl (1H-Benzimidazol-2- yl)carbamate (Carbendazim) Request your copy now! MM Imp. B (EP): 2-Chloro-6,7-dimethoxyquinazolin- 4-amine LGC Quality ISO Guide 34 GMP/GLP ISO 9001 ISO/IEC ISO/IEC MM A 100mg S A 100mg N H N N N H OO N H N OO N H O O N H O NH 2 O Imp. D (EP) as Dihydrochloride: N-(4-Amino-6,7- dimethoxyquinazolin-2-yl)-n-methylpropane-1,3- diamine Dihydrochloride O N Cl A 100mg Visit us at 6 O N n/a A 50mg 2 Also available from LGC Standards: Pharmaceutical impurities and primary reference standards 2014 NH 2 NH 2 N NH 2 Print_LGC_Katalog_MM_ _NoHyperlink_v04j.pdf 6 20/01/ :55:39 A comprehensive catalogue of more than 3,000 pharmaceutical impurities and primary reference standards manufactured by LGC. Contact your local LGC Standards office for your free copy, or download a PDF version of this catalogue at Visit us at LGC Quality - ISO Guide 34 GMP/GLP ISO 9001 ISO/IEC ISO/IEC LGC Limited, All rights reserved. LGC Standards is part of the LGC Group. 3970/LB/0514

65 Other services Closed customised PT schemes Trial test materials Scheme development Special bespoke test materials Wish list Other PT services Reference materials Training and education

66 Closed customised PT schemes In addition to the regular schemes, LGC Standards is able to offer customised or closed proficiency testing schemes tailored to a specific organisation s requirements. We operate such schemes on behalf of some of the largest companies in the world and we are happy to discuss your requirements in this area. Please note that a minimum level of business is required before a scheme can be developed. For further details contact: [email protected] Trial test materials LGC Standards constantly aims to improve and add value to the services we offer. As new laboratory methods and legislations are introduced worldwide, LGC Standards understands that laboratories need to prove their competence in an ever growing range of techniques and legislative requirements. Our trial test materials allow laboratories to fulfil this requirement and because the trials test materials are not included in our scope of accreditation it allows us to be more flexible when developing and modifying our products. Special bespoke test materials In the unlikely event that our standard schemes do not fulfil your company s needs, we would be happy to discuss any special requests you may have, subject to a minimum order value. Please download the special request form from the website and send the completed form to: [email protected] Wish list If our current range of PT schemes and trials does not meet your requirements we welcome your suggestions for new analytes, test material, or a whole new scheme. Contact us directly by completing the Wish list form on our website Please complete a form for each new idea you have and ensure all mandatory fields are filled. The Wish list form will provide us with the information we need to review your idea at our development meetings. Scheme development LGC Standards is continually striving to improve current schemes and introduce new test materials and schemes where appropriate. Other PT services Advice and consultancy for potential PT providers Consultancy and training for PT providers in the implementation of appropriate quality systems Training courses for PT participants and their customers Prominent role in the development of policy and guidance for proficiency testing by representing the UK on a number of key international committees 66 Tel: +44 (0) Fax: +44 (0)

67 Reference materials LGC Standards combines expertise in analytical sciences, and experience in international standards, to offer laboratories worldwide the ideal solution to sourcing high quality certified reference materials. Our expert technical support staff have unrivalled knowledge in the use of certified reference materials across a broad range of analytical sectors. As the leading global supplier of reference materials, LGC Standards offers: Partnership with the world s key producers of reference materials Expert local customer service and technical support Supply of all major pharmacopeia and reference materials Assistance in finding the right materials to suit your need Over 100,000 reference materials in our range For further information or to receive any one of our catalogues, please contact your local office or visit our website: Training and education At LGC, providing our customers with high quality analytical products and services is an integral part of what defines the organisation. As such we are committed to providing first-rate training courses for analytical scientists. Delegates attend from a diverse range of sectors including environmental analysis, food analysis, pharmaceuticals and chemical manufacturing. With over 15 years experience in delivering analytical quality training programmes worldwide, our team of professionals will help ensure you meet current accreditation and regulatory requirements and give your customers confidence in the quality of the data you produce. Our trainers have extensive experience of analytical quality assurance which ensures that our training remains topical and can be tailored to meet the needs of individual organisations. Examples of topics covered include: Understanding ISO/IEC requirements for analytical laboratories Statistics for analytical scientists Method validation Evaluating measurement uncertainty Proficiency testing The courses are run regularly at LGC s Teddington site and can also be delivered off-site. We recognise that the needs of each individual company may differ so a customised solution is often required. If you have specific requirements, or you have a group of staff who require training, then a bespoke or on-site course may be the most cost effective and practical answer. If you wish to discuss your training requirements or, to make a booking, please contact: LGC training centre Tel: +44 (0) [email protected] [email protected] Website: 67

68 DRUG SOLUTIONS NOW DEA EXEMPT drug reference materials Drug solutions and powders produced to ISO Guide 34. Benzodiazepines Analgesics Barbiturates New psychoactive substances Amphetamines Opiates Cannabinoids Cocaine Antidepressants Antifungals Antiepileptics Antipsychotics And many more LGC Quality - ISO Guide 34 GMP/GLP ISO 9001 ISO/IEC ISO/IEC LGC Limited, All rights reserved. LGC Standards is part of the LGC Group. LoGiCal is a registered trademark of LGC Standards GmbH. 3664/LB/0713

69 Frequently asked questions

70 Q: Which international standards are relevant to LGC PT schemes? A: All our PT schemes are operated in accordance with the international standard ISO/IEC The statistical analysis undertaken is in accordance with the international standard ISO LGC is accredited by the United Kingdom Accreditation Service (UKAS) for the provision of proficiency testing schemes against ISO/IEC 17043; a copy of our current scope of accreditation which lists the accredited schemes is available on our website: Q: How are your schemes organised? A: The day-to-day operation of each scheme is the responsibility of LGC Standards. Individual schemes are managed by a team of Scheme Coordinators, to cover reporting, customer service and technical functions. For some schemes, external advisors may also be used to provide the full range of relevant knowledge and expertise needed to operate the scheme effectively. A small number of schemes are run in collaboration with other organisations. Q: Do you use Advisors and Advisory Groups? A: Yes, depending upon the scheme in question. Advisors are selected on the basis of their technical knowledge and experience of the industry to which the scheme is related. Advisors may be used on an ad-hoc basis and contacted when specific issues need to be addressed. Alternatively, formal Advisory Groups may be used. Advisory Groups consist of members who may or may not be participants on the scheme but who are experienced in the field of testing covered by the PT scheme. The composition and terms of reference of each Advisory Group will be agreed on a scheme-by-scheme basis. Membership of the Advisory Groups is subject to change but members names are available on request. Q: Do you run schemes that are jointly managed? A: Yes, some schemes are operated jointly with a partner organisation. Where schemes are operated jointly, a Management Committee may be set up to address operational issues for the scheme. Q: How do I join a scheme? A: Participants are advised to take part in the scheme(s) that are most fitting to their own area of testing. Where necessary, appropriate staff at LGC Standards can advise on which scheme is most suitable for participants. For each scheme, a scheme description and application form will be available, containing information about the test materials included in the scheme, and the intended distribution dates. Participants are invited to complete the application form, indicating which test materials they wish to receive during the scheme year. If the availability of test materials changes during the scheme year, participants are kept fully informed. Once a completed application form is received, an order confirmation will be sent to the participant, confirming the test materials selected and distribution date. Q: Can you guarantee my laboratory s confidentiality? A: In order to ensure confidentiality, participants in all schemes are allocated a unique laboratory reference number. This number enables results to be reported without divulging the identities of participant laboratories. Only staff within the proficiency testing team and the laboratory itself will know this number. Q: How often do I need to participate? A: The frequency that a laboratory needs to participate in proficiency testing depends on a wide range of factors specific to each individual laboratory, such as other quality tools used, the volume of work undertaken and the risk associated to the measurements. Therefore every individual laboratory may have a different need, which is why schemes provided by LGC Standards offer flexible participation, although some do have a minimum participation level. Third parties, such as regulatory bodies, may recommend minimum levels of participation. To gain the benefit from trend analysis, participation in a minimum of four rounds over a scheme year is normally recommended. 70 Tel: +44 (0) Fax: +44 (0)

71 Q: What are the fees for participation? A: Fees for participation are reviewed annually and the current fees for each scheme are available on application. Payment terms are detailed on the application form and invoice. Participants are advised that delays with payments may result in test materials and / or reports being withheld until payments are made. Q: Where do you source your test materials? A: The vast majority of test materials are manufactured by LGC Standards. Where this is not possible, test materials are carefully sourced to meet the needs of participants. Wherever practical, test materials will be as similar as possible to those routinely tested by participating laboratories. However, in some cases, in order to achieve the required degree of homogeneity and stability, test materials may be in the form of simulated matrices or concentrated spiking solutions. The analyte concentration range of test materials will usually be varied from round to round in order to be realistic and challenging. Details of individual test material types are available in the relevant scheme description. Q: How are test materials packaged and transported? A: Test materials are packaged appropriately to protect the contents during transit. The majority of test materials are sent using priority courier. Overseas customers must provide relevant documents to prevent delay in customs such as import permits and may be required to pay import duties locally. Once packages have been delivered, LGC Standards cannot be held responsible if they subsequently fail to reach the correct personnel or are not stored under the recommended conditions. Participants are asked to check the contents of packages immediately on receipt and to contact LGC Standards if there are any problems with the condition of the test materials or accompanying documentation. Q: How is test material stability affected by time, distance and temperature? A: The test materials are all stable at the stated storage temperatures for at least the period of the proficiency test round. Studies have shown there is no significant difference between results of test materials tested the day after despatch and those tested on the deadline date. There is also no evidence that results are influenced by different climatic conditions of participating countries. Distance travelled does not affect test material results. We have undertaken studies on a number of our PT test materials comparing the average result according to distance travelled, and no correlations have been found. Stability consideration is an important part of the design and feasibility process for a PT scheme, where transport conditions such as temperature, humidity, pressure, exposure to x-rays etc are taken into account. Q: How do I treat my PT test material? A: It is important for laboratories to understand how to get the optimum benefit from PT participation. To do this, a laboratory must participate in an open and honest fashion, being prepared to, on occasion, be evaluated as unsatisfactory. If PT is to achieve its aims, laboratories need to treat the PT test materials the same as routine test materials, and staff must be encouraged to treat them appropriately and learn from their results in a constructive manner. Laboratories will learn very little about the quality of their routine work if the PT test materials are given special treatment, such as carrying out a much higher number of analyses, in order to be evaluated as satisfactory. This may in fact compromise the quality of routine measurements as a disproportionate level of effort is being expended for the PT. [email protected] Website: 71

72 Q: Do I have to use specific methods to analyse the test materials? A: Unless otherwise instructed, participants should analyse the test materials using any method that they feel is appropriate. Participants are asked to treat the PT material in the same way as a routine test material. Participants may be asked to state their method when reporting results. It is important that this information is accurate as results are analysed and reported according to the method stated. Q: Do I have to report my results within a specific timescale? A: Deadlines are specified for the return of results, to ensure the timely issue of assigned values and reports to participants. For each scheme a closure date will therefore be specified. For certain tests there may also be a date specified by which examination of the test material is recommended to have been commenced. This is to ensure that sufficient time is available to complete the test and report results in time for the deadline date. Q: How should I report my results using PORTAL? A: For the majority of schemes, results are returned through our bespoke electronic reporting software, PORTAL. Once you are ready to report your results, please go to: You will need to log in using your lab ID, username and password. We advise that prior to using PORTAL you read the user guide which is available at: select help from the menu. If you require further assistance please contact our support team: Tel: +44 (0) [email protected] or your local LGC Standards office. For some schemes (or parts of a scheme) alternative reporting mechanisms are provided, details of which will be ed to participants prior to test materials receipt. It is recommended that results and calculations are checked thoroughly before reporting. Results should be reported clearly, in the format and units detailed in the scheme description. If calculations are used, unless instructed otherwise, the laboratory is to report only the final calculated result. In general, results of zero should not be reported; results should be reported depending upon the detection limit of the method used, for example, <10. The exception is a small number of parameters, where it may be appropriate to report a result of zero, depending on the measurement scale being used. Results of zero and truncated results, such as < or > cannot often be included in the data analysis and therefore allocated a performance score. Results will be rounded up or down to the number of reporting decimal places stipulated in the scheme description and may not therefore be identical to your original reported result. The effects of rounding may also mean that occasionally percentage totals do not add up exactly to 100 %. Part of the challenge of proficiency testing is the ability to perform calculations and transcribe results correctly. The proficiency testing team cannot interpret or calculate results on participants behalf. Once submitted and received, results cannot be amended and no changes can be made after the report has been issued. However, if you notice an error in your result before the reporting deadline, this can be corrected using PORTAL until the round closes. Q: How many results may I submit? A: Although it is desirable for participants to submit multiple results in order to compare results between different analysts, methods or instruments, a single laboratory reporting a large number of results could potentially bias the dataset. In order to minimise the effects of bias, LGC Standards therefore limits the number of results participants are able to report. Each participant is able to enter up to 13 different results. Of these results a maximum of 3 results can be nominated results. Nominated results are included in the statistical analysis of the dataset whilst non-nominated results are not, however all results will receive z performance scores and assessments as appropriate. Nominated results must be obtained using different methods, again to minimise the effects of bias. 72 Tel: +44 (0) Fax: +44 (0)

73 Further information is available in the PORTAL User Guide and the PORTAL Nominated Results FAQ, both of these documents are available for download from the PORTAL website and further information is available from Q: Can my results be included in the report if I ve missed the deadline for reporting? A: Participants are asked to return results by the given deadline in order to ensure that their results are included in the statistical analysis and the scheme report. Results received after the closure date will not be included in the report. For schemes where a generic report is issued, this is available to all participants subscribing to the round regardless of whether their results were submitted or not. Q: How do you prevent collusion and falsification of results? A: It defeats the objective of taking part in proficiency testing if participants are not returning genuine results. Certain measures are built into the schemes to try and prevent collusion but, ultimately the responsibility rests with each participating laboratory to behave in a professional manner. Q: How is the assigned value established? A: ISO 13528: Statistical Methods for use in Proficiency Testing by Interlaboratory Comparisons sets out how the assigned value and performance assessment criteria can be established and describes the options for the various performance scoring systems. The assigned value is the value selected as being the best estimate of the true value for the parameter under test. The method used to determine the assigned value may vary depending upon the particular scheme and test material and is detailed in the relevant scheme description. and technically feasible approach to use. When the assigned value is determined from the consensus value of participant results, or from expert laboratories, robust statistical methods are used for calculation of the consensus value, the estimated standard uncertainty and the robust standard deviation. For qualitative tests, participant results are compared against the intended result (assigned value) based on formulation or expert assessment. For interpretive schemes where the result is subjective rather than quantifiable, a model answer produced by appropriate experts will be published in the report. For microbiology test materials, all participant results are transformed by converting them to log 10 before the statistical analysis is undertaken. Q: How do I evaluate measurement uncertainty? A: The aim when evaluating measurement uncertainty is to combine the effects of all the errors that will influence the measurement result, into a single value. There are many different guides available which provide advice on evaluating measurement uncertainty. There are two specific guides that are internationally recognised: ISO (BIPM, IEC, IFCC, IUPAC, IUPAP and OIML) Guide to the Expression of Uncertainty in Measurement EURACHEM/CITAC Guide Quantifying Uncertainty in Analytical Measurement (available at: Further information on approaches to evaluating measurement uncertainty may also be available from your national accreditation body. For quantitative tests, where it is appropriate, practicable and technically feasible, the assigned value will be derived through formulation (or occasionally through the use of a certified reference material) to provide metrological traceability; the associated uncertainty of the value can therefore be estimated. However, in many cases the use of a consensus value is the only practicable [email protected] Website: 73

74 Q: Can I use PT data to estimate my measurement uncertainty? A: It is possible, but must be regarded as a very rough estimate, and is not an approach addressed in many guides to evaluating measurement uncertainty. However documents that do address the use of PT data are: EURACHEM/CITAC Guide Quantifying Uncertainty in Analytical Measurement (available at NORDTEST Report TR 537 Handbook for Calculation of Measurement Uncertainty in Environmental Laboratories ISO/TS Microbiology of Food and Animal Feeding Stuffs - Guidelines for the Estimation of Measurement Uncertainty for Quantitative Determinations Q: What is the Standard Deviation for Proficiency Assessment (SDPA)? A: The SDPA expresses the acceptable difference between the laboratory result and the assigned value. An acceptable z performance score represents a result that does not deviate from the assigned value by more than twice the SDPA. The method used to determine the SDPA may vary depending upon the particular scheme and test material and is detailed in the relevant scheme description. Q: How do I report a presumptive result in microbiology? A: Report your result as usual but record in the comments section that the result is Presumptive. Q: What is the purpose of scoring my result? A: Once the assigned value for the parameters under test has been established, participant laboratories are assessed on the difference between their result and the assigned value, with this difference being represented by a performance score called a z score. This provides a simple and consistent measure of performance which is the key to monitoring competence and implementing an improvement programme as required. Q: How is a z perfomance score calculated? A: The participant s result, x, is converted into a z performance score using the following formula: z = (x - X) SDPA Where: X = the assigned value SDPA = Standard Deviation for Proficiency Assessment For small data sets, there will be increased uncertainty around the assigned value if derived from a consensus value from participants results. In such cases, z performance scores may not be provided, or may be given for information only. The z score expresses performance in relation to the assigned value and the standard deviation for proficiency assessment (SDPA). A z performance score of 2 represents a result that is a distance of 2 x SDPA from the assigned value. A fit for purpose value for SDPA, rather than being derived from participant results, is preferable as this enables z scores to be compared from round to round to demonstrate general trends. For each scheme, the value of SDPA and the method used to derive it is reported in the scheme description and / or report. Q: How do I interpret my results? A: For quantitative examinations, participant performance is assessed using the z score, and the following interpretation is given to results: z 2.00 Satisfactory result 2.00 < z < 3.00 Questionable result z 3.00 Unsatisfactory result For qualitative examinations or semi-qualitative results, laboratories reporting the assigned result or range of results will be considered correct, and therefore have satisfactory performance. 74 Tel: +44 (0) Fax: +44 (0)

75 Q: What are the advantages of a z performance score? A: Results can be expressed in a form that is easy to interpret and understand Results can be summarised in graphical or tabular form to depict overall performance A performance score allows participants to directly compare their own result with others If consistent statistical values are applied, a performance score enables participants to monitor and trend their own performance over time It is important to interpret any performance score in the full context of the overall results and in the context of a laboratory s own quality control measures. Q: What is the estimated uncertainty of the assigned value? A: The assigned value has a standard uncertainty (u x ) that depends upon the method used to derive the assigned value. When the assigned value is determined by the consensus of participants results, the estimated standard uncertainty of the assigned value can be calculated by: u x = 1.25 x Robust standard deviation/ n Where n = number of results When the assigned value is determined by formulation, the standard uncertainty is estimated by the combination of uncertainties of all sources of error, such as gravimetric and volumetric measurements. If u x is 0.3 x SDPA, then the uncertainty of the assigned value can be considered negligible and need not be considered in the interpretation of results. If u x is > 0.3 x SDPA, then the uncertainty of the assigned value is not negligible in relation to the SDPA and so z performance scores (z-prime), which takes into account the standard uncertainty of the assigned value in their calculation, will be reported in place of z performance scores. Q: How is z performance score (z-prime) calculated? A: A z performance score (z-prime) incorporates the standard uncertainty of the assigned value and is calculated as follows: z = (x - X) SDPA 2 + u x 2 Where: x = participant result X = the assigned value SDPA = Standard Deviation for Proficiency Assessment u x = standard uncertainty of the assigned value X A z performance score is interpreted in exactly the same way as a z performance score, 2 is satisfactory, >2 but <3 is questionable and 3 is unsatisfactory. Q: Do you include outlying results due to errors and blunders in the statistical analysis of the data? A: Although robust estimators are used in order to minimise the influence of outlying results, extreme results or results that are identifiably invalid should not be included in the statistical analysis of the data. For example, these may be results caused by calculation errors or the use of incorrect units. However, such results can be difficult to identify by the PT organiser. For this reason, the robust mean and standard deviation will be calculated in the usual way, but those results that are out of the range of the assigned value ± 5 x SDPA will be excluded and the robust mean and standard deviation will then be recalculated. These recalculated values will be used for the statistical analysis. By removing these blunders from the dataset any influence on the summary statistics is completely removed. All results, including excluded results, will be given performance scores. [email protected] Website: 75

76 Q: What could be the cause of my poor performance? A: A single poor result is not indicative of overall laboratory performance but neither is a single good result. Ideally, PT results should be monitored over time to detect potential bias or repeated unsatisfactory results. There are many possible reasons for a single poor result. It is therefore important to interpret the results from PT schemes within the context of an all-round quality assurance programme, including internal quality control, use of validated methods and reference materials. There are numerous potential causes of poor performance in a PT scheme which may include analytical and non-analytical errors. Analytical errors Calibration / instrument problems Extraction / clean-up Interferences / matrix effects Diagnostic kits / reagents Analyst / method performance Non-analytical errors Calculation / transcription Reporting format / units Poor / incorrect storage Test material defects Test materials are subjected to rigorous quality control testing before being distributed to participants, and are unlikely to be the cause of a poor z performance score. All possible reasons for a poor performance should be investigated fully in order to identify the most likely cause and to enable action to be taken to prevent recurrence. Repeat test materials are available after every distribution, but it is most important to investigate and understand the reason(s) for the failure, document this fully, and carry out corrective actions before repeating a test. Q: How can I measure my laboratory s performance over time? A: You can do this by trend analysis. A single result simply reflects the performance of the laboratory on the particular day that the test was carried out and therefore gives limited information. Frequent participation in PT schemes over time can give greater insight into long-term performance and can help identify where internal bias may be occurring. One of the best methods of summarising performance scores over time is graphically as this gives a clear overview and is less prone to misinterpretation than numerical methods. Participants are therefore advised to monitor their PT results over time. An online trend analysis tool is included in the cost of your PT participation with LGC. The online tool is built into the PORTAL reporting system and allows you to quickly plot your results over a range of rounds and easily download the charts for further circulation. More information regarding interpretation and trend analysis of proficiency results is given in the Eurachem guide on Selection, Use and Interpretation of Proficiency Testing (PT) schemes (available at IUPAC International Harmonised Protocol for the Proficiency Testing of Analytical Chemistry Laboratories, 2006 and ISO Q: How can I graphically plot and analyse trends for qualitative results? A: Qualitative results are difficult to depict graphically as they are not normally allocated a performance score. However for qualitative results, a correct result could be allocated a performance score of 0 to represent a satisfactory result. A false positive result can be represented by a performance score of + 3, whilst a false negative result can be represented by a performance score of - 3. If plotted graphically over time, this should give a clear visual indicator of performance in qualitative tests. Q: How will I receive my report? A: Following statistical evaluation of the results, the reports will generally be available on the website within 4 to 10 working days of round closure (see specific scheme description). We aim to provide 95 % of our reports to participants within 5 working days. Participants will be ed when the report is available. The content of reports vary from scheme to scheme 76 Tel: +44 (0) Fax: +44 (0)

77 but include details of the composition of test materials, the assigned values, and tabular and / or graphical representations of participants results. Paper copies are also available for an additional charge. Q: How do I assess the reproducibility standard deviation from the PT report? A: The robust standard deviation provided in the PT report for a specific method can be taken as an estimate of the reproducibility standard deviation for the PT round for that specific method. Q: Can I have a report that only includes my group laboratories? A: Yes, we can produce reports tailored to a customer s specific requirement. There may be an additional charge for administration and computer programming costs. Copyright to all reports remains with LGC Standards but permission is granted to participants to make copies for their own internal use, for example for quality control and regulatory purposes. No other copies may be made without obtaining permission. Q: My results have not been included in the report can I calculate my performance z / z performance score? A: To calculate your performance score please visit: Select help from the menu and download the z / z performance score calculator. Q: How can I receive advice and feedback? A: Communication with participants will be carried out through scheme-related documentation, s, letters, or through local LGC Standards offices. Open meetings may also be organised and all interested parties invited to attend. Q: How can I send feedback? A: Comments on any aspect of our products and services are welcome either by phone, fax, letter, or by contacting your local LGC Standards office. Q: Can I suggest a scheme or test material? A: We welcome suggestions any time. Please complete the Wish list form on our website: [email protected] Website: 77

78 Quality control materials Post-report PT test materials available At the end of PT rounds, material is available for up to 3 months to the participants in order to investigate any issues with the analytical process. This is often required by accreditation bodies where there is no obvious non-analytical error (such as a transcription error) that could explain the occurrence of a poor score in a PT round. Value quality control materials For many of our schemes there is ample stock available after the report has been issued, and these are ideal for use as quality control materials within the day-to-day laboratory programme. For many schemes, the samples are commercially produced materials with long shelf-lives, making them the perfect solution for many of your QC requirements. Proven quality The assigned value for the PT test materials is supported by the analysis of many laboratories (in some cases hundreds) and statistical data processing, to ensure the reported values are reliable*. Benefits of PT test materials for quality control Proven values, fully documented in the PT report Excellent value for money Ideal for method validation May be used to help establish measurement uncertainty Perfect for process control between PT rounds Excellent training tool for new staff Saves time preparing in-house controls Helps validate internally prepared materials Underpins your internal quality procedures For further information contact: LGC Standards Proficiency Testing 1 Chamberhall Business Park, Chamberhall Green, Bury, Lancashire BL9 0AP Tel: +44 (0) Fax: +44 (0) [email protected] Web: *Some materials use reference values and these are determined by different processes, depending upon the scheme. Please check the specific scheme report for the material required.

79 Index of analytes

80 Analyte Scheme Matrix **Sample 1,1,1,2-Tetrachloroethane CONTEST Standard solution Group C, 19 Soil Group C, 25* 1,1,1-Trichloroethane AIR Charcoal filled glass sorbent tubes (Workplace air) 6* AQUACHECK Groundwater 7B CONTEST Standard solution Group C, 19 19B Soil Group C, 25* 1,2,3-Trichlorobenzene AQUACHECK Groundwater 7B 1,2,4-Trichlorobenzene AQUACHECK Groundwater 7B 1,2,4-Trimethylbenzene (TMB) AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) 1,2-Dichloroethane AQUACHECK Groundwater 6A Groundwater CONTEST Standard solution Group C, 19 19B 19B 21* 7B 18A 19B Soil Group C, 25* 1,2-Dichloroethene CONTEST Soil Group C, 25* 1,3,5-Trichlorobenzene AQUACHECK Groundwater 7B 12:0 Lauric acid AFPS Oil or fat 3 19B QFCS Oil or fat 778* 14:0 Myristic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 14:1 n-5 Myristoleic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 15:0 Pentadecanoic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 16:0 Palmitic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 16:1 Palmitoleic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 17:0 Heptadecanoic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 18:0 Stearic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 18:1 cis-9 Oleic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 18:2 n-6 Linoleic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 18:3 n-3 Linolenic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 20:0 Eicosanoic arachidic acid AFPS Oil or fat 3 QFCS Oil or fat 778* 20:1 Eicosenoic acid AFPS Oil or fat 3 20:5 n-3 Eicosapentaenoic acid AFPS Oil or fat 3 22:6 n-3 Docosahexaenoic acid AFPS Oil or fat Methylbutanol BAPS Lager 2L * Not included in our scope of accreditation **See current application form and scheme description 80 Tel: +44 (0) Fax: +44 (0)

81 Analyte Scheme Matrix **Sample Methylbutanol (cont.) DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Liqueur Group E, E2 Cream liqueur Group E, E3 2,3,6-TBA AQUACHECK Groundwater 8* 20* 2,4,5-T AQUACHECK Groundwater 8* Groundwater 20* 2,4,6-Trichlorophenol AQUACHECK Groundwater 6B 18B 2,4-D AQUACHECK Groundwater ,4-DB AQUACHECK Groundwater ,4-Dichlorophenol AQUACHECK Groundwater 6B 18B 2,5-Dimethylphenol AQUACHECK Groundwater 6B* 18B* 2-Chlorophenol AQUACHECK Groundwater 6B 2-Chlorotoluene CONTEST Standard solution Group C, 19 18B Soil Group C, 25* 2-Methylbutanol BAPS Lager 2L DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Liqueur Cream liqueur 3-Methylbutanol BAPS Lager 2L Group B, B2 Group B, B3 Group E, E2 Group E, E3 DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Liqueur Cream liqueur 2-Methylphenol (o-cresol) AQUACHECK Groundwater 6B* 18B* 3-Methylphenol (m-cresol) AQUACHECK Groundwater 6B* 18B* 4-Methylphenol (p-cresol) AQUACHECK Groundwater 6B* 18B* 3,5-Dimethylphenol AQUACHECK Groundwater 6B* 18B* 3-Bromophenol AQUACHECK Groundwater 6B* 18B* 4-Chlorophenol AQUACHECK Groundwater 6B 4-Phenylcycohexene (4-PCH) AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) Acenaphthene AQUACHECK Groundwater 7C Group B, B2 Group B, B3 Group E, E2 Group E, E3 18B 21* * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 81

82 Analyte Scheme Matrix **Sample Acenaphthene (cont.) AQUACHECK 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Acenaphthylene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Acesulfame K QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 Acesulfame K (E950) QFCS Liquid 761 Acetal DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Liqueur Group E, E2 Cream liqueur Group E, E3 Acetaldehyde BAPS Lager 2L DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Liqueur Group E, E2 Cream liqueur Group E, E3 Acetic acid DAPS Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Acetone TOX Blood TAK Acid and base number OIL #2 Diesel fuel 2* Acid and base titration PHARMASSURE Acid solution 1B Acidity (as Citric acid monohydrate) QBS Fruit juice 510 Carbonated drinks 515 Dilutable and ready to drink materials 517 Acidity OIL Aviation turbine fuel (Jet A) 3* QFCS Bread or cake 776 Acid number (Total) OIL Crude oil 4* Acid number OIL Engine oil lubricants 5* Acoustic testing TOYTEST Toy product 13 Actual alcoholic strength DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Simulated spirit Group B, B4 Ciders Group C, C1 White or Rosé wine Group D, D1 Red wine Group D, D2 Ready to drink Group E, E1 Liqueur Group E, E2 Cream liqueur Group E, E3 Simulated liqueur Group E, E4 Acid detergent fibre (ADF) AFPS Animal feed 1 Advanced testing PHARMASSURE Sample format will depend upon test type 2 Additive elements OIL Engine oil lubricants 5* Aerobic mesophilic bacteria detection COSMETICS Powder 10 Cream 13 * Not included in our scope of accreditation **See current application form and scheme description 82 Tel: +44 (0) Fax: +44 (0)

83 Analyte Scheme Matrix **Sample Aerobic mesophilic bacteria detection (cont.) COSMETICS Liquid 16 Aerobic mesophilic bacteria count COSMETICS Powder 10 Cream 13 Liquid 16 Aerobic psychrotrophs count QMS Oatmeal 25 Aflatoxin B1 AFPS Animal feed 5 QFCS Nuts 779* Aflatoxin B2 AFPS Animal feed 5 QFCS Nuts 779* Aflatoxin G1 AFPS Animal feed 5 QFCS Nuts 779* Aflatoxin G2 AFPS Animal feed 5 QFCS Nuts 779* Aflatoxin M1 QDCS Freeze dried milk 60* Aflatoxins (Total) AFPS Animal feed 5 Alcohol DAPS Fermented wort Group A, A1 Simulated wort Group A, A2 QUARTZ Blood 4 Alcohol by volume BAPS Ale (Bitter) 1B Lager 1L Alcohol in blood alcohol technical defence (ATD) QUARTZ Paper exercise 5* Aldrin AQUACHECK Groundwater 7A 19A Alkalinity AQUACHECK Hard water 1H Soft water 1S Allura red (E129) QFCS Liquid 763 Alpha amylase MAPS Brewers and distillers malt 1,1 Alpha endosulphan AQUACHECK Groundwater 7A 19A Alpha hexachlorocyclohexane AQUACHECK Groundwater 7A Aluminium AQUACHECK Natural water 4 TOYTEST Groundwater 19A 4G 12 Industrial wastewater EN71-3 Standard solution and real material Ametryn AQUACHECK Groundwater 8B* 17C 20B* Amfetamines and stimulants DAH Hair DH01*, DH02* DAU Urine 1,2,3 DOF Oral fluid OF1, OF2, OF3 Amfetamine QUARTZ Blood 3 Amikacin TDM Human serum AT03 Amiodarone TDM Human serum CRD Amisulpride TDM New born calf serum PS24 Amitriptyline QUARTZ Blood 3 Amitriptyline and nortriptyline TDM New born calf serum PS01 Ammonia AIR Impinger solution (Stack emissions) 37 AQUACHECK Hard water 2H 3 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 83

84 Analyte Scheme Matrix **Sample Ammonia (cont.) AQUACHECK Soft water 2S Spiking solution 10 Industrial wastewater 17D CONTEST Soil Group B, 3b Soil Group D, 18 AMPA AQUACHECK Groundwater 8 20 Anaerobic sulphite-reducing bacteria count QMS Oatmeal 15 Skimmed milk powder 15 Anaesthetic drugs QUARTZ Blood 1 Blood and case study 2 Anthracene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Anisidine value QFCS Oil or fat 778* Anticholinergic drugs QUARTZ Blood 1 Blood and case study 2 Anticonvulsant drugs QUARTZ Blood 1 Blood and case study 2 Antidepressant drugs QUARTZ Blood 1 Blood and case study 2 Antihistamine drugs QUARTZ Blood 1 Blood and case study 2 Antibiotics (Beta lactam based) QDCS Freeze dried milk 34 (A,B and C) Antibiotics (Penicillin and sulfur based) QDCS Freeze dried milk 57 (A,B and C) Antimony AIR Metals impinger solution (Stack emissions) 33 Metals and fly ash on quartz filter 47mm diameter (Stack emissions) AQUACHECK Natural water 5 Natural water Groundwater Industrial wastewater 38* 5A 5G 17C 12* CONTEST Standard solution Group A, 1b Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 QBS Fruit juice 518 TOYTEST Soft drink 519 EN71-3 Standard solution and real material Antipsychotic drugs QUARTZ Blood 1 Blood and case study 2 AOX AQUACHECK Wastewater 27 3 a-pinene AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) 21* Apparent alcoholic strength DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 * Not included in our scope of accreditation **See current application form and scheme description 84 Tel: +44 (0) Fax: +44 (0)

85 Analyte Scheme Matrix **Sample Apparent alcoholic strength (cont.) DAPS Clear distilled spirit Group B, B3 Simulated spirit Group B, B4 Appparent gravity BAPS Ale (Bitter) 1B Lager 1L Aqueous tritium AQUACHECK Clean water 31 Aripiprazole and Dehydroaripiprazole TDM New born calf serum PS25 Aroma - Alcoholic / Solvent BAPS Lager / ale (Bitter) 5 Aroma - Burnt BAPS Lager / ale (Bitter) 5 Aroma - Caramel BAPS Lager / ale (Bitter) 5 Aroma - Cereal BAPS Lager / ale (Bitter) 5 Aroma - DMS BAPS Lager / ale (Bitter) 5 Aroma - Fruity / Citrus BAPS Lager / ale (Bitter) 5 Aroma - Fruity / Estery BAPS Lager / ale (Bitter) 5 Aroma - Hop BAPS Lager / ale (Bitter) 5 Aroma - Malty BAPS Lager / ale (Bitter) 5 Aroma - Other BAPS Lager / ale (Bitter) 5 Aroma - Other sulfur BAPS Lager / ale (Bitter) 5 Aroma - Oxidised / Aged BAPS Lager / ale (Bitter) 5 Aroma - Sweet BAPS Lager / ale (Bitter) 5 Arsenic AFPS Animal feed 2 AIR Quartz fibre filter 47 mm diameter (Ambient air) Metals impinger solution (Stack emissions) Metals and fly ash on quartz filter 47mm diameter (Stack emissions) 13* 33 38* AQUACHECK Natural water 5 Natural water Groundwater 5A 5G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1b Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 PHARMASSURE Powder material 2B* QFCS Dried tea 780* QMAS Shellfish 741 Fish 742 Meat 747* SUPS Cane or beet sugar 2 TOYTEST EN71-3 Standard solution and real material Ascorbic acid DAPS White or Rosé wine Group D, D1 Red wine Ready to drink Ascorbic acid (Vitamin C) QBS Liquid test material Group D, D2 Group E, E1 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 85

86 Analyte Scheme Matrix **Sample Ash OIL #2 Diesal fuel 2* Engine oil lubricants 5* QCS Cocoa powder 716 QDCS Skimmed milk powder 38A Whey powder 38B Whole protein concentrate 43 QFCS Cereal 770 'Ready to eat' product 772 Flour 775 Bread or cake 776 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Fish 734 SUPS Cane or beet sugar 1 Raw sugar 7 Ash Insoluble in hydrochloric acid AFPS Animal feed 1 Ash sulfated OIL Engine oil lubricants 5* Asphaltenes OIL Crude oil 4* Aspartame QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 QFCS Liquid 761 Atrazine AQUACHECK Groundwater 8B 20B Azinphos-ethyl AQUACHECK Groundwater 9 21 Azinphos-methyl AQUACHECK Groundwater 9 21 Azo - Dyes TOYTEST Toy material 8 Bacillus cereus count QMS Oatmeal 17 Skimmed milk powder 17 Barbiturate drugs QUARTZ Blood 1 Blood and case study 2 Base number OIL Engine oil lubricants 5* Barium AQUACHECK Hard water 1H Soft water 1S Natural water 4 Groundwater 4G Industrial wastewater 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 TOYTEST Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 EN71-3 Standard solution and real material 3 Benazolin AQUACHECK Groundwater 8* 20* Bentazone AQUACHECK Groundwater 8 20 * Not included in our scope of accreditation **See current application form and scheme description 86 Tel: +44 (0) Fax: +44 (0)

87 Analyte Scheme Matrix **Sample Benz(a)anthracene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Benz(a)pyrene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Benzene AIR Charcoal filled glass sorbent tubes (Workplace air) 5* Tenax TA filled sorbent tubes (Workplace air) 7* Tenax TA filled sorbent tubes (Ambient air) 12* Tenax TA filled sorbent tubes (Indoor or Chamber air) 21* AQUACHECK Groundwater 6C 18C CONTEST Standard solution Group C, 15 Soil 22* Benzo(b)fluoranthene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Benzo(b/k)fluoranthene (Sum) CONTEST Soil Group C, 3c Standard solution Group C, 5 Benzo(ghi)perylene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Benzo(k)fluoranthene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Benzodiazepine DAH Hair DH01*, DH02* Benzoic acid DAPS Ready to drink Group E, E1 QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 Benzoic acid (E210 - E213) QFCS Liquid 760 Benzodiazepine drugs QUARTZ Blood 1 Blood and case study 2 Beryllium AQUACHECK Natural water 5 Groundwater 5G CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Beta endosulphan AQUACHECK Groundwater 7A 19A Beta glucan MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 Beta hexachlorocyclohexane AQUACHECK Groundwater 7A 19A * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 87

88 Analyte Scheme Matrix **Sample Bifidobacterium species count QMS Lyophilised test material 27 Bitterness BAPS Ale (Bitter) 1B Lager Ale 3 BOCLE lubricity OIL Aviation turbine fuel (Jet A) 3* BOD (5 day) AQUACHECK Spiking solution 3 1L Spiking solution 11 Boiled wort colour MAPS Malt - EBC wort dependent 1,2 Boron AQUACHECK Natural water 4 Groundwater Industrial wastewater 4G 17C CONTEST Soil Group D, 18 TOYTEST EN71-3 Standard solution and real material Boron (Total) AQUACHECK Sewage sludge 13 Agricultural soil 14 BP distribution OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Breath alcohol (Alcohol technical defence - ATD) QUARTZ Paper exercise 5* BRF 8ml MAPS Barley 2 Brilliant blue (E133) QFCS Liquid 763 Brix DAPS Ready to drink Group E, E1 QBS Fruit juice 510 Carbonated drinks 515 Dilutable and ready to drink materials 517 Bromacil AQUACHECK Groundwater 8* 20* Bromate AQUACHECK Spiking solution 3A Bromide AQUACHECK Spiking solution 3A Bromodichloromethane AQUACHECK Groundwater 6A Bromoform AQUACHECK Groundwater 6A Bromoxynil AQUACHECK Groundwater A 18A 20 Bulk density QDCS Whole protein concentrate 43 Buprenorphine TDM Human serum SA01 Butyl acetate AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) Butyric acid QCS Chocolate 715 Cadmium AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* 21* Quartz fibre filter 47mm diameter (Ambient air) 13* Metals impinger solution (Stack emissions) 33 Metals and fly ash on quartz filter 47mm diameter (Stack emissions) 38* AQUACHECK Natural water 5 Natural water 5B Groundwater 5G 12 * Not included in our scope of accreditation **See current application form and scheme description 88 Tel: +44 (0) Fax: +44 (0)

89 Analyte Scheme Matrix **Sample Cadmium (cont.) AQUACHECK Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 COSMETICS Lipstick 19 Lip gloss 20 Powder 21 PHARMASSURE Powder material 2B* QBS Fruit juice 518 Soft drink 519 QFCS Dried tea 780* QMAS Shellfish 741 TOYTEST Fish 742 Meat 747* EN71-3 Standard solution and real material Cadmium (Total) TOYTEST Paint flakes 9 Caffeine QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 TDM Human serum TD1 Calcium AFPS Animal feed 2 AQUACHECK Hard water 1H Soft water 3 1S Sewage sludge 16 BAPS Lager 2L Brewing liquor 6A* CONTEST Soil Group D, 18 DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit QBS Fruit juice 518 Soft drink 519 QDCS Milk 28 Hard cheese 37 Whole milk powder 41 Soft cheese 59 QFCS Flour 775 Bread or cake 776 QMAS Precooked, raw or processed meat 731 Campylobacter species count QMS Lyophilised test material 32* Campylobacter species detection QMAS Lyophilised meat 743 QMS Lyophilised test material 21 Candida albicans count PHARMASSURE Lyophilised test material 4 Candida albicans detection PHARMASSURE Lyophilised test material 4 Group B, B2 Group B, B3 Cannabinoids DAH Hair DH01*, DH02* * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 89

90 Analyte Scheme Matrix **Sample Cannabinoids (cont.) DAU Urine 1,2,3 DOF Oral fluid OF1, OF2, OF3 Cannabinoid drugs QUARTZ Blood 1 Blood and case study 2 Carbamazepine TDM Human serum TD1 Carbamazepine + CBZ-epoxide TDM Human serum TD1 Carbetamide AQUACHECK Groundwater 8B* 20B* Carbohydrate QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Carbohydrate (Total) BAPS Lager 2L Carbohydrate (Total and available) QFCS Cereal 770 'Ready to eat' product 772 Carbon dioxide BAPS Ale (Bitter) 1B Lager 1L DAPS Ciders Group C, C1 Ready to drink Group E, E1 QBS Carbonated drinks 515 Carbon, hydrogen and nitrogen OIL #2 Diesel fuel 2* Carbon residue OIL #2 Diesel fuel 2* Carbon tetrachloride AQUACHECK Groundwater 6A Groundwater 7B 18A 19B Carbonate content AQUACHECK Agricultural soil 14 Carboxyhaemoglobin TOX Human blood BLD Carboxyhaemoglobin drugs QUARTZ Blood 1 Blood and case study 2 Cardiovascular drugs QUARTZ Blood 1 Blood and case study 2 CBZ-epoxide TDM Human serum TD1 Chloramphenicol TDM Human serum AT05 Chlorate (High level) AQUACHECK Spiking solution 3A Chlorate (Low level) AQUACHECK Spiking solution 3A Chlorfenvinphos AQUACHECK Groundwater 9 21 Chloride AFPS Animal feed 2 AQUACHECK Hard water 1H Soft water Higher salinity potable water 1S 1A Spiking solution 10 BAPS Lager 2L CONTEST Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 QDCS Whole milk powder 42 QFCS Bread or cake 776 Chlorine (Free) AQUACHECK Natural water 3B Chorine (Total) AQUACHECK Natural water 3C * Not included in our scope of accreditation **See current application form and scheme description 90 Tel: +44 (0) Fax: +44 (0)

91 Analyte Scheme Matrix **Sample Chlorite (High level) AQUACHECK Spiking solution 3A Chlorite (Low level) AQUACHECK Spiking solution 3A Chlorobenzenes (sum of 1,2,4- and 1,2,3-trichlorobenzene) CONTEST Standard solution Group C, 19 Soil Group C, 25* Chloroform AQUACHECK Groundwater 6A Groundwater Chlorophyll α AQUACHECK Clean water 33* Chlorpyrifos AQUACHECK Groundwater 9 7B 18A 19B 21 Chlortoluron AQUACHECK Groundwater 8B Chromium AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* 20B Metals impinger solution (Stack emissions) 33 Metals and fly ash on quartz filter 47mm diameter (Stack emissions) AQUACHECK Natural water 5 Groundwater 38* 5G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 COSMETICS Lipstick 19 TOYTEST Lip gloss 20 Powder 21 EN71-3 Standard solution and real material 3 Chromium (III) TOYTEST EN71-3 Standard solution and real material 3 Chromium (VI) AQUACHECK Clean water 5C Synthetic wastewater 12C CONTEST Soil Group A, 3a TOYTEST Standard solution Group A, 13 Soil Group D, 18 EN71-3 Standard solution and real material Chromium (Total) AQUACHECK Natural water 5B Chrysene AQUACHECK Groundwater 7C 3 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Citalopram QUARTZ Blood 3 Citalopram and norcitalopram TDM New born calf serum PS18 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 91

92 Analyte Scheme Matrix **Sample Citric acid DAPS Simulated spirit Group B, B4 White or Rosé wine Red wine Ready to drink Simulated liqueur Clobazam TDM Human serum CN1 Clomipramine and norclomipramine TDM New born calf serum PS03 Clonazepam TDM Human serum TD1 Clopyralid AQUACHECK Groundwater 8* 20* Clostridium perfringens count QMAS Lyophilised meat 738 QMS Oatmeal 10 Skimmed milk powder 10 QWAS Potable water 413 Clostridium perfringens detection QGS Gelatine hydrolysate 603 Clostridium species detection QMS Oatmeal 10 Skimmed milk powder 10 Cloud point OIL #2 Diesel fuel 2* Clozapine and norclozapine TDM Human serum PS04 Coagulase-positive staphylococci count QMAS Lyophilised meat 738 Lyophilised fish or shellfish 739 QMS Oatmeal 17 Skimmed milk powder 17 Tea 29 QWAS Bathing, recreational and surface water 421 Coagulase-positive staphylococci detection QWAS Mineral water 424 Cobalt AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* Metals impinger solution (Stack emissions) Group D, D1 Group D, D2 Group E, E1 Group E, E4 33 Metals and fly ash on quartz filter 47mm diameter (Stack emissions) 38* AQUACHECK Natural water 5 Groundwater 5G Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a TOYTEST Soil extract Group A, 2 Soil EN71-3 Standard solution and real material Group A, 3a Cocaine and metabolites DAH Hair DH01*, DH02* DAU Urine 1,2,3 DOF Oral fluid OF1, OF2, OF3 Codeine QUARTZ Blood 3 COD AQUACHECK Spiking solution 3 Spiking solution 11 CONTEST Soil Group D, 18 QDCS Potassium hydrogen phthalate 35 COD (High) AQUACHECK Spiking solution 29 3 * Not included in our scope of accreditation **See current application form and scheme description 92 Tel: +44 (0) Fax: +44 (0)

93 Analyte Scheme Matrix **Sample COD (Low) AQUACHECK Spiking solution 29 COD (Total) AQUACHECK Wastewater 17A BAPS Effluent simulant solution 6B* Cold filter plugging point OIL #2 Diesel fuel 2* Cold water extract MAPS Brewers and distillers malt 1,1 Coliforms count QCS Cocoa powder 713 Chocolate 717* QMAS Lyophilised meat 735 QMS Oatmeal 16 Skimmed milk powder 16 Oatmeal 18 Skimmed milk powder 18 Tea 29 QWAS Potable water 412 Coliforms detection QGS Gelatine hydrolysate 602 QMS Oatmeal 18 Skimmed milk powder 18 QWAS Potable water 425 Colony identification and counting techniques QMIS Photograph and paper exercise 103 Colour AQUACHECK Hard water 2H Soft water 2S DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Ciders Group C, C1 MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 Black Malt 4 Crystal malt 4 OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Engine oil lubricants 5* QBS Dilutable and ready to drink materials 517 QFCS Oil or fat 778* SUPS Cane or beet sugar 1 Raw sugar 7 Colour at 420nm DAPS White or Rosé wine Group D, D1 Red wine Group D, D2 Colour at 430nm BAPS Ale (Bitter) 1B Lager 1L Ale 3 Colour at 520nm DAPS White or Rosé wine Group D, D1 Red wine Group D, D2 Colour at 530nm BAPS Ale 3 Colour at 620nm DAPS White or Rosé wine Group D, D1 Combined chorine AQUACHECK Natural water 3C Combustion (Net heat) OIL Aviation turbine fuel (Jet A) 3* Complex cyanide CONTEST Soil Group B, 3b Conductivity AQUACHECK Agricultural soil 14 Conductivity (20 C) AQUACHECK Hard water 1H Hard water 2H * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 93

94 Analyte Scheme Matrix **Sample Conductivity (20 C) (cont.) AQUACHECK Soft water 1S Soft water Higher salinity potable water Wastewater 2S 1A 17A BAPS Brewing liquor 6A* CONTEST Soil Group D, 18 Copper AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* Metals impinger solution (Stack emissions) 33 Metals and fly ash on quartz filter 47mm diameter (Stack emissions) 38* AQUACHECK Natural water 4 Groundwater Natural water 4G 5B 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater BAPS Lager 2L 17C Brewing liquor 6A* CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit White or Rosé wine Red wine QDCS Whole milk powder 41 SUPS Cane or beet sugar 2 TOYTEST EN71-3 Standard solution and real material Copper corrosion OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Group B, B2 Group B, B3 Group D, D1 Group D, D2 Cresols CONTEST Soil Group C, 3c Standard solution 3 Group C, 7b Cresols (Total) CONTEST Standard solution Group C, 7b Cronobacter species detection QMS Skimmed milk powder 4 Crude ash AFPS Animal feed 1 Crude fat AFPS Animal feed 1 Crude fibre AFPS Animal feed 1 Crude protein AFPS Animal feed 1 Cyanazine AQUACHECK Groundwater 8B* 20B* Cyanide AQUACHECK Industrial wastewater 17B Cyanide (Free) AQUACHECK Hard water 2H Soft water 2S * Not included in our scope of accreditation **See current application form and scheme description 94 Tel: +44 (0) Fax: +44 (0)

95 Analyte Scheme Matrix **Sample Cyanide (Free) (cont.) AQUACHECK Spiking solution 10 CONTEST Soil Group B, 3b Soil Group D, 18 Cyanide (Total) AQUACHECK Hard water 2H Soft water 2S Spiking solution 10 CONTEST Soil Group B, 3b Standard solution Group B, 6 Soil Group D, 18 Cyclohexanone AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) Cypermethrin AQUACHECK Groundwater 9 21* 21 Daphnia magna 24hr EC50 AQUACHECK Ecotoxicology 50 Daphnia magna 48hr EC50 AQUACHECK Ecotoxicology 50 Degrees of crystallisation MAPS Crystal malt 4 Delta hexachlorocyclohexane AQUACHECK Groundwater 7A Density OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Engine oil lubricants 5* PHARMASSURE Standard material 1D Density, relative density or API gravity OIL Crude oil 4* Deoxynivalenol (DON) MAPS Malt flour 3 Desethylamiodarone TDM Human serum CRD Dextran SUPS Raw sugar 7 Diacetyl as VDK BAPS Lager 2L Ale 3 Diastatic power (DPWK) MAPS Brewers and distillers malt 1,1 Diazepam QUARTZ Blood 3 Diazinon AQUACHECK Groundwater 9 19A 21 Dibenz(ah)anthracene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Dibromochloromethane AQUACHECK Groundwater 6A Dicamba AQUACHECK Groundwater 8 18A 20 Dichlobenil AQUACHECK Groundwater 8* 20* Dichloromethane CONTEST Standard solution Group C, 19 Soil Group C, 25* Dichlorprop AQUACHECK Groundwater 8 20 Dichlorvos AQUACHECK Groundwater 9 21 Dieldrin AQUACHECK Groundwater 7A 19A * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 95

96 Analyte Scheme Matrix **Sample Dietary fibre QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Diflufenican AQUACHECK Groundwater 8B* 20B* Digoxin TDM Human serum TD1 Dimethyl disulfide BAPS Lager 2L Dimethyl sulfide BAPS Lager 2L Dipotassium hydrogen phosphate (Purity) PHARMASSURE Standard solution 1C Dimethyl sulfide precursor (DMSP) MAPS Brewers and distillers malt 1,1 Malt - EBC wort dependent 1,2 Dissolution PHARMASSURE Sample format varies for each round 2C* Dissolved organic carbon (DOC) AQUACHECK Spiking solution 3 CONTEST Solid waste Group E, 21 Soil Group E, 24* Dissolved / Total organic carbon AQUACHECK Spiking solution 11 Distillation OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Distillable phenolic substances CONTEST Soil Group C, 3c Diuron AQUACHECK Groundwater 8B Dodecane AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) 20B 21* Dothiepin and northiaden TDM New born calf serum PS19 Doxepin and nordoxepin TDM New born calf serum PS05 Dry matter CONTEST Soil Group B, 3b Dry substance SUPS Molasses 5 Dulcoside A SUPS Stevia 9* Duloxetine TDM New born calf serum PS27 Easily liberated sulfide CONTEST Soil Group B, 3b Standard solution Group B, 12 EDDP TDM Human serum SA02 Elemental sulfur CONTEST Soil Group C, 3c Eluable fat AFPS Oil or fat 3 Endrin AQUACHECK Groundwater 7A 19A Energy QFCS Cereal 770 'Ready to eat' product 772 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Energy value (kcal) BAPS Lager 2L Energy value (kj) BAPS Lager 2L Enterobacteriaceae count AFPS Animal feed 7 COSMETICS Powder 10 Cream 13 Liquid 16 QCS Cocoa powder 713 Chocolate 717* QMAS Lyophilised meat 735 Lyophilised fish or shellfish 739 QMS Oatmeal 16 Skimmed milk powder 16 * Not included in our scope of accreditation **See current application form and scheme description 96 Tel: +44 (0) Fax: +44 (0)

97 Analyte Scheme Matrix **Sample Enterobacteriaceae count (cont.) QMS Oatmeal 18 Skimmed milk powder 18 Enterobacteriaceae detection QMS Oatmeal 18 Skimmed milk powder 18 Enterococci count QCS Cocoa powder 713 Chocolate 717* Enterococci (faecal streptococci) count QMS Oatmeal 9 Skimmed milk powder 9 QWAS Potable water 412 Bathing, surface and wastewater 419 Mineral water 420 Sea water 422 Enterococci (faecal streptococci) detection QWAS Potable water 425 Erectile dysfunction drugs QUARTZ Blood 1 Blood and case study 2 Escherichia coli count PHARMASSURE Lyophilised test material 4 QMAS Lyophilised meat 735 Lyophilised fish or shellfish 739 QMS Oatmeal 16 Skimmed milk powder 16 Oatmeal 18 Skimmed milk powder 18 Oatmeal 20 Skimmed milk powder 20 QWAS Potable water 412 Effluent sludge 416 Bathing, surface and wastewater 419 Mineral water 420 Sea water 422 Escherichia coli detection COSMETICS Powder 10 Cream 13 Liquid 16 PHARMASSURE Lyophilised test material 4 QBS Fruit juice 500 Soft drink 501 QGS Gelatine hydrolysate 602 QMS Oatmeal 18 Skimmed milk powder 18 QWAS Potable water 425 Escherichia coli detection (Filtration) QBS Lyophilised test material 505 Escherichia coli O157 (non toxigenic strain) detection QMS Oatmeal 22 Skimmed milk powder 22 Escitalopram TDM New born calf serum PS28 Ethanol TOX Human serum SM Blood Human blood Urine TAK BLD URN Ethanol (Alcohol in blood) QUARTZ Blood 4 Ethosuximide TDM Human serum TD1 Ethyl acetate BAPS Lager 2L * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 97

98 Analyte Scheme Matrix **Sample Ethyl acetate (cont.) DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Liqueur Cream liqueur Ethyl benzene AIR Charcoal filled glass sorbent tubes (Workplace air) Tenax TA filled sorbent tubes (Workplace air) Tenax TA filled sorbent tubes (Ambient air) Group B, B2 Group B, B3 Group E, E2 Group E, E3 5* 7* 12* AQUACHECK Groundwater 6C 18C CONTEST Standard solution Group C, 15 Soil Group C, 22* Ethyl carbamate DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Simulated spirit Group B, B4 Ethyl glucuronide DAU Urine 1,2,3 Ethylene glycol TOX Blood TAK Evaporating loss OIL Engine oil lubricants 5* Ethyl hexanoate BAPS Lager 2L Extract 0.2mm MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 Extract 0.7mm MAPS Malt - IoB wort dependent 1,3 Extract 1.0mm MAPS Malt - EBC wort dependent 1,2 Extract difference ( ) MAPS Malt - IoB wort dependent 1,3 Extract difference ( ) MAPS Malt - EBC wort dependent 1,2 Extractable phosphorus AQUACHECK Agricultural soil 14 Extraction of magnesium AQUACHECK Agricultural soil 14 Extraction of potassium AQUACHECK Agricultural soil 14 Extraction of sodium AQUACHECK Agricultural soil 14 Faecal coliforms count QWAS Bathing, surface and wastewater 419 Sea water 422 Fat QCS Chocolate 715 Cocoa powder 716 QDCS Skimmed milk 27 Hard cheese 37 Whey powder 38B Whipping cream 39 Semi skimmed milk 40 Whole protein concentrate 43 Skimmed milk powder 51 Whole milk powder 52 Single cream 53 Double cream 54 Whole milk 55 Yogurt 58 Soft cheese 59 QFCS Cereal 770 * Not included in our scope of accreditation **See current application form and scheme description 98 Tel: +44 (0) Fax: +44 (0)

99 Analyte Scheme Matrix **Sample Fat (cont.) QFCS Ready to eat product 772 Flour 775 Bread or cake 776 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Fish 734 Fat (Total) QMAS Meat 733 Fatty acids profile (Absolute) AFPS Oil or fat 3 Fatty acids profile (Relative) AFPS Oil or fat 3 Felbamate TDM Human serum AE2 Fenitrothion AQUACHECK Groundwater 9 21 Fenthion AQUACHECK Groundwater 9 21 Fermentability (Boiled) MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 Fermentability (Unboiled 0.2mm) MAPS Malt - IoB wort dependent 1,3 Fermentability (Unboiled 0.7mm) MAPS Malt - IoB wort dependent 1,3 Final gravity DAPS Fermented wort Group A, A1 Simulated wort Finger paint TOYTEST EN71-7 paper exercise 4 Fish authenticity QMAS Fish 751* Fish speciation QMAS Fish 752* Flammability TOYTEST EN71-2 Toy product and or section of material Flash Point OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Engine oil lubricants 5* Flecainide TDM Human serum CRD Flucytosine TDM Human serum AT06 Fluoranthene AQUACHECK Groundwater 7C Group A, A2 2 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Fluorene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Fluoride AQUACHECK Hard water 1H Soft water 1S Sewage sludge 13 Agricultural soil 14 CONTEST Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 QUARTZ Blood 4 Fluoride (Total) CONTEST Standard solution Group B, 17 Fluoxetine and norfluoxetine TDM New born calf serum PS06 Fluphenazine TDM New born calf serum PS07 Fluroxypyr AQUACHECK Groundwater 8* 20* * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 99

100 Analyte Scheme Matrix **Sample Fluvoxamine TDM New born calf serum PS22 Flux testing TOYTEST Magnets 14* Foam stability (HRV) BAPS Lager 2L Formaldehyde2,4-DNPH derivative AIR Glass fibre filter 25mm diameter (Workplace air) 8* Formaldehyde AQUACHECK Spiking solution 28 Fraction IV (<2.2mm + damaged corns from all other sieves) MAPS Brewers and Distillers malt 1,1 Barley 2 Free 2,3-Pentanedione BAPS Lager 2L Ale 3 Free alpha amino nitrogen (FAN) BAPS Lager 2L MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 Free diacetyl BAPS Lager 2L Ale 3 Free dimethyl sulfide (DMS) MAPS Brewers and Distillers malt 1,1 Free fatty acids AFPS Oil or fat 3 QFCS Oil or fat 778* Freeze point OIL Aviation turbine fuel (Jet A) 3* Freezing point depression (FPD) QDCS Milk 56 Freshwater algae growth inhibition test biomass reduction (Pseudokirschneriella subcapitata) AQUACHECK Ecotoxicology 50 Friability MAPS Brewers and distillers malt 1,1 Fructose DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Simulated spirit White or Rosé wine Red wine QBS Fruit juice 510 Carbonated drinks 515 Dilutable and ready to drink materials 517 Group B, B2 Group B, B3 Group B, B4 Group D, D1 Group D, D2 Furfural DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Liqueur Cream liqueur Gabapentin TDM Human serum AE1 Gammahydroxybutyrate (GHB) DAU Urine 1,2,3 TOX Urine GHB Gentamicin and vancomycin TDM Human serum AT01 Germinative capacity MAPS Barley 2 Germinative energy MAPS Barley 2 Glassy (Whole) corns MAPS Brewers and distillers malt 1,1 Glucose BAPS Lager 2L Group B, B2 Group B, B3 Group E, E2 Group E, E3 DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Simulated spirit White or Rosé wine Red wine Group B, B2 Group B, B3 Group B, B4 Group D, D1 Group D, D2 * Not included in our scope of accreditation **See current application form and scheme description 100 Tel: +44 (0) Fax: +44 (0)

101 Analyte Scheme Matrix **Sample Glucose (cont.) QBS Fruit juice 510 Carbonated drinks 515 Dilutable and ready to drink materials 517 Glycerol DAPS Simulated spirit Group B, B4 Simulated liqueur Glycosidic nitrile MAPS Brewers and distillers malt 1,1 Glyphosate AQUACHECK Groundwater 8 20 Gross alpha as 241 Americium AQUACHECK Clean water 30 Gross alpha as 239 Plutonium AQUACHECK Clean water 30 Gross alpha as 230 Thornium AQUACHECK Clean water 30 Gross beta as 137 Caesium AQUACHECK Clean water 30 Gross beta as 40 Potassium AQUACHECK Clean water 30 Gross beta as 90 Strontium AQUACHECK Clean water 30 Guaiacol producing thermophilic acidophilic bacteria detection SUPS Lyophilised test material 8 Gum OIL Aviation turbine fuel (Jet A) 3* Haloperidol TDM New born calf serum PS14 Hardness (As carbonate) BAPS Brewing liquor 6A* Hartong VZ45 MAPS Brewers and distillers malt 1,1 Group E, E4 Haze DAPS Scotch whisky Group B, B1 Dark distilled spirit Haze at 0 C BAPS Ale (Bitter) 1B Haze at 20 C BAPS Ale (Bitter) 1B Headspace air QBS Carbonated drinks 515 Heat content OIL #2 Diesel fuel 2* Hectolitre weight MAPS Barley 2 Heptachlor AQUACHECK Groundwater 7A Ciders Lager Lager Heptachlor epoxide AQUACHECK Groundwater 7A Hexachlorobenzene AQUACHECK Groundwater 7A Hexachloro-1,3-butadiene AQUACHECK 19A Group B, B2 Group C, C1 CONTEST Soil Group C, 25* Hexachlorobutadiene AQUACHECK Groundwater 7B High temperature simulated distillation (HTSD) OIL Crude oil 4* High temperature stability OIL #2 Diesel fuel 2* Histamine QMAS Fish 748* Homogeneity MAPS Brewers and distillers malt 1,1 HPLC PHARMASSURE Sample format varies for each round 2A HTHS viscosity OIL Engine oil lubricants 5* Hydrocarbon type(s) OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Hydrogen chloride AIR Impinger solution (Stack emissions) 36 Hydrogen content OIL Aviation turbine fuel (Jet A) 3* Hydrogen fluoride AIR Impinger solution (Stack emissions) 35 Hydrogen sulfide BAPS Lager 2L 1L 1L 19A 19A 19B * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 101

102 Analyte Scheme Matrix **Sample Hydroquinone COSMETICS Cream 22 Hydroxyproline QMAS Meat 733 Hypnotic drugs QUARTZ Blood 1 Blood and case study 2 Identity of organism BAPS Lyophilised test material 4 H Lyophilised test material 4 L PHARMASSURE Lyophilised test material 5 Imipramine and desipramine TDM New born calf serum PS02 Indeno(1,2,3-cd)pyrene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Indigo carmine (E132) QFCS Liquid 762 Insolubility index QDCS Whole protein concentrate 43 Skimmed milk powder 51 Whole milk powder 52 Insoluble impurities AFPS Oil or fat 3 Iodine value QFCS Oil or fat 778* Ioxynil AQUACHECK Groundwater 8 20 Iron AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* AQUACHECK Natural water 4 Groundwater 4G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C BAPS Brewing liquor 6A* Lager 2L CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 White or Rosé wine Group D, D1 Red wine Group D, D2 QBS Fruit juice 518 Soft drink 519 QDCS Whole milk powder 42 QFCS Cereal 770 Flour 775 Bread or cake 776 SUPS Cane or beet sugar 2 Iso-alpha-acids BAPS Lager 2L Ale 3 Iso-amyl acetate BAPS Lager 2L DAPS Scotch whisky Group B, B1 * Not included in our scope of accreditation **See current application form and scheme description 102 Tel: +44 (0) Fax: +44 (0)

103 Analyte Scheme Matrix **Sample Iso-amyl acetate (cont.) DAPS Dark distilled spirit Group B, B2 Clear distilled spirit Liqueur Cream liqueur Group B, B3 Group E, E2 Group E, E3 Iso-butanol BAPS Lager 2L DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Liqueur Group E, E2 Cream liqueur Group E, E3 Iso-propyl alcohol (IPA) TOX Blood TAK Isoproturon AQUACHECK Groundwater 8B 20B Kinetic energy testing TOYTEST Toy product 12 Kjeldahl nitrogen AQUACHECK Hard water 1H Soft water 1S Spiking solution 10 Kolbach index MAPS Malt - EBC wort dependent 1,2 Lacosamide TDM Human serum AE2 Lactic acid DAPS White or Rosé wine Group D, D1 Red wine Group D, D2 Lactic acid bacteria count BAPS Lyophilised test material 4 H Lyophilised test material 4 L QBS Fruit juice 500 Soft drink 501 QMAS Lyophilised meat 746 QMS Oatmeal 24 Skimmed milk powder 24 Lactic acid bacteria count (Filtration) QBS Lyophilised test material 505 Lactose QDCS Milk 28 Whey powder 38B Whole protein concentrate 43 Lamotrigine TDM Human serum TD1 Lead AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* Quartz fibre filter 47mm diameter (Ambient air) Metals impinger solution (Stack emissions) Metals and fly ash on quartz filter 47mm diameter (Stack emissions) 13* 33 38* AQUACHECK Natural water 5 Natural water 5B Groundwater 5G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 103

104 Analyte Scheme Matrix **Sample Lead (cont.) CONTEST Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 COSMETICS Lipstick 19 Lip gloss 20 Powder 21 PHARMASSURE Powder material 2B* QBS Fruit juice 518 Soft drink 519 QFCS Dried tea 780* QMAS Shellfish 741 Fish 742 Meat 747* SUPS Cane or beet sugar 2 TOYTEST EN71-3 Standard solution and real material Lead (Total) TOYTEST Paint flakes 9 Legionella pneumophila count by culture QWAS Environmental water 417 Legionella pneumophila count by PCR QWAS Environmental water 417 Legionella pneumophila detection QWAS Environmental water 417 Legionella pneumophila identification QWAS Environmental water 417 Legionella species count by culture QWAS Environmental water 418 Legionella species count by PCR QWAS Environmental water 418 Legionella species detection QWAS Environmental water 418 Legionella species detection (Low level) QWAS Potable water 423 Legionella species identification QWAS Environmental water 418 Levetiracetam TDM Human serum AE1 3 Limonene AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) 21* Linalool DAPS Clear distilled spirit Group B, B3 Lindane (Gamma HCH) AQUACHECK Groundwater 7A 19A Linuron AQUACHECK Groundwater 8B 20B Listeria monocytogenes count QMS Oatmeal 8 Skimmed milk powder 8 Listeria monocytogenes detection QMAS Lyophilised meat 737 QMS Oatmeal 7 Skimmed milk powder 7 Listeria species count QMS Oatmeal 8 Skimmed milk powder 8 Listeria species detection QMAS Lyophilised meat 737 QMS Oatmeal 7 Skimmed milk powder 7 Lithium AQUACHECK Natural water 4 Groundwater 4G TDM Human serum TD1 Loss on ignition AQUACHECK Agricultural soil 14 CONTEST Soil Group B, 3b * Not included in our scope of accreditation **See current application form and scheme description 104 Tel: +44 (0) Fax: +44 (0)

105 Analyte Scheme Matrix **Sample Loss on ignition (500±5ºC) AQUACHECK Sewage sludge 16 Low level count of microorganism PHARMASSURE Lyophilised test material 3 Low level identification of microorganism PHARMASSURE Lyophilised test material 3 Lubricity (HFRR) wear scar diameter at 25ºC OIL #2 Diesel fuel 2* Luminescent bacteria IC50 tests (15 minutes) AQUACHECK Ecotoxicology 50 Magnesium AFPS Animal feed 2 AQUACHECK Hard water 1H Soft water Higher salinity potable water 1S 1A Sewage sludge 16 BAPS Brewing liquor 6A* Lager CONTEST Soil Group D, 18 DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit QBS Fruit juice 518 2L Soft drink 519 QDCS Whole milk powder 42 Magnesium (Concentration) PHARMASSURE Standard solution 1C Malathion AQUACHECK Groundwater 9 21 Group B, B2 Group B, B3 Malic acid DAPS White or Rosé wine Group D, D1 Red wine Malt mod homogeneity MAPS Brewers and distillers malt 1,1 Malt modification MAPS Brewers and distillers malt 1,1 Maltose BAPS Lager 2L Group D, D2 DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Simulated spirit Maltotetraose BAPS Lager 2L Maltotriose BAPS Lager 2L Manganese AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* Metals impinger solution (Stack emissions) Group B, B2 Group B, B3 Group B, B4 33 Metals and fly ash on Quartz filter 47mm diameter (Stack emissions) 38* AQUACHECK Natural water 4 Groundwater 4G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil QDCS Whole milk powder 42 TOYTEST EN71-3 Standard solution and real material Group A, 3a 3 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 105

106 Analyte Scheme Matrix **Sample Maprotiline and normaprotiline TDM New born calf serum PS12 Mass of solids AIR Dust on Glass fibre filter 25mm diameter (Workplace air) Dust on glass fibre filter 37mm diameter (Workplace air) Dust on glass fibre filter 47mm diameter (Stack emissions) 3* 4* 40* MCPA AQUACHECK Groundwater 8 20 MCPB AQUACHECK Groundwater 8 20 Measurement testing TOYTEST Various 11 Meat authenticity QMAS Beef, chicken, horse, lamb or pork 749* Mechanical and physical properties TOYTEST EN71-1 Toy product for paper exercise 1 ASTM F963 Toy product for paper exercise 7 Mecoprop AQUACHECK Groundwater 8 20 Melting point PHARMASSURE Standard material 1F Mercaptan sulfur OIL Aviation turbine fuel (Jet A) 3* Mercury AFPS Animal feed 2 AIR Impinger solution (Stack emissions) 31 Impinger solution (Stack emissions) 32 AQUACHECK Natural water 5 Natural water Groundwater 5B 5G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1c Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 PHARMASSURE Powder material 2B* QFCS Dried tea 780* QMAS Shellfish 741 TOYTEST Fish 742 Meat 747* EN71-3 Standard solution and real material Mesophilic aerobic bacterial spores count QMS Oatmeal 11 Skimmed milk powder 11 Mesophilic anaerobic spores count QMS Oatmeal 15 Skimmed milk powder 15 Metals (vanadium, nickel, iron) OIL Crude oil 4* Metaldehyde AQUACHECK Groundwater 8 20 Methanethiol BAPS Lager 2L Methanol DAPS Scotch whisky Group B, B1 3 * Not included in our scope of accreditation **See current application form and scheme description 106 Tel: +44 (0) Fax: +44 (0)

107 Analyte Scheme Matrix **Sample Methanol (cont.) DAPS Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Liqueur Group E, E2 Cream liqueur Group E, E3 Metamitron AQUACHECK Groundwater 8B* 20B* Metazachlor AQUACHECK Groundwater 8* 20* Methabenzthiazuron AQUACHECK Groundwater 8B* 20B* Methadone QUARTZ Blood 3 TDM Human serum SA02 Methanol TOX Blood TAK Methotrexate TDM Human serum TD1 Methylene blue active substances (MBAS) AQUACHECK Spiking solution 3 Spiking solution 11 Methyl isobutyl ketone (MIBK) AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) 21* Methylthioacetate BAPS Lager 2L Mevinphos AQUACHECK Groundwater 9 21 Micro carbon residue OIL Crude oil 4* Minor tranquillizers DAU Urine 1,2,3 DOF Oral fluid OF1, OF2, OF3 Mirtazapine and normirtazapine TDM New born calf serum PS11 Moisture AFPS Animal feed 1 Oil or fat 3 MAPS Brewers and distillers malt 1,1 Barley 2 Crystal malt 4 Black malt 4 PHARMASSURE Sample format will depend upon test type 2 QCS Chocolate 715 Cocoa powder 716 QDCS Butter 36 Hard cheese 37 Skimmed milk powder 38A Whey powder 38B Whole protein concentrate 43 Whole milk powder 52 Soft cheese 59 QFCS Cereal 770 'Ready to eat' product 772 Flour 775 Bread or cake 776 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Fish 734 SUPS Raw sugar 7 Molybdenum AQUACHECK Natural water 5 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 107

108 Analyte Scheme Matrix **Sample Molybdenum (cont.) AQUACHECK Groundwater 5G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 Monohydric phenols (By distillation) CONTEST Standard solution Group C, 7a Mono-unsaturates QMAS Meat 733 Monuron AQUACHECK Groundwater 8B Morphine QUARTZ Blood 3 Mould count AFPS Animal feed 7 PHARMASSURE Lyophilised test material 4 20B QBS Fruit juice 500 Soft drink 501 QCS Cocoa powder 713 Chocolate 717* QMAS Lyophilised meat 746 QMS Oatmeal 23 Skimmed milk powder 23 QWAS Process water 414 SUPS Lyophilised test material 6 Mould count (Filtration) QBS Lyophilised test material 505 m-xylene AQUACHECK Groundwater 6C n-butanol DAPS Scotch whisky Group B, B1 Dark distilled spirit Clear distilled spirit Liqueur Cream liqueur n-butyl acetate AIR Charcoal filled glass sorbent tubes (Workplace air) 18C Group B, B2 Group B, B3 Group E, E2 Group E, E3 6* n-hexane AIR Charcoal filled glass sorbent tubes (Workplace air) Tenax TA filled sorbent tubes (Indoor or Chamber air) 6* 21* n-propanol BAPS Lager 2L DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Liqueur Group E, E2 Cream liqueur Group E, E3 Naphthalene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 * Not included in our scope of accreditation **See current application form and scheme description 108 Tel: +44 (0) Fax: +44 (0)

109 Analyte Scheme Matrix **Sample Naphthalene (cont.) OIL Aviation turbine fuel (Jet A) 3* Netilimicin TDM Human serum AT04 Neutral detergent fibre (NDF) AFPS Animal 1 New psychoactive substances QUARTZ Blood 6* Nickel AIR Cellulose filter (Workplace air) 1* Quartz fibre filter 47mm diameter (Ambient air) Metals impinger solution (Stack emissions) Metals and fly ash on quartz filter 47mm diameter (Stack emissions) 13* 33 38* AQUACHECK Natural water 5 Natural water Groundwater 5B 5G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 COSMETICS Lipstick 19 TOYTEST Lip gloss 20 Powder 21 EN71-3 Standard solution and real material 3 Nickel release NiMS Alloy disks, jewellery or other appropriate articles 1A*,1B*,1C* Nicotine and cotinine TDM Lyophilised human urine NC01* Nitrate AQUACHECK Hard water 2H Soft water 2S Spiking solution 10 BAPS Lager 2L CONTEST Soil Group D, 18 QMAS Lyophilised meat 732 Nitrite AQUACHECK Hard water 2H Soft water 2S Spiking solution 10 QMAS Lyophilised meat 732 Nitrogen OIL Engine oil lubricants 5* Nitrogen (Total) AQUACHECK Spiking solution 10 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17D MAPS Brewers and distillers malt 1,1 Barley 2 OIL Crude oil 4* QCS Chocolate 715 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 109

110 Analyte Scheme Matrix **Sample Nitrogen dioxide AIR Palmes-type diffusion tubes (Ambient air) 11* Nitrosodimethylamine (NDMA) DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Simulated spirit Group B, B4 MAPS Brewers and distillers malt 1,1 Malt flour 5 Non filterable COD AQUACHECK Wastewater 17A BAPS Effluent simulant solution 6B* Non-ionic surfactants AQUACHECK Spiking solution 3 Non-opiate narcotics DAU Urine 1,2,3 DOF Oral fluid OF1, OF2, OF3 Non steroidal anti-inflammatory analgesic drugs QUARTZ Blood 1 Blood and case study 2 Norbuprenorphine TDM Human serum SA01 Norclobazam TDM Human serum CN1 Ochratoxin AFPS Animal feed 5 Ochratoxin A MAPS Malt flour 3 OH-oxcarbazepine TDM Human serum AE1 Olanzapine TDM Human serum PS15 o,p'-ddd (TDE) AQUACHECK Groundwater 7A* 19A* o,p'-dde AQUACHECK Groundwater 7A* 19A* o,p-ddt AQUACHECK Groundwater 7A 19A Opiates DAH Hair DH01*, DH02* DAU Urine 1,2,3 DOF Oral fluid OF1, OF2, OF3 Opioid analgesic drugs QUARTZ Blood 1 Blood and case study 2 Organic carbon content AQUACHECK Agricultural soil 14 Organic chemical compounds TOYTEST EN71-9 paper exercise 6 Original extract BAPS Ale (Bitter) 1B Lager 1L Original gravity BAPS Ale (Bitter) 1B Lager 1L DAPS Fermented wort Group A, A1 Simulated wort Group A, A2 Osmophilic mould count QMS Oatmeal 5 Skimmed milk powder 5 SUPS Lyophilised test material 6 Osmophilic yeast count QMS Oatmeal 5 Skimmed milk powder 5 SUPS Lyophilised test material 6 Other 30 min luminescent bacteria IC50 tests AQUACHECK Ecotoxicology 50 Other substances of interest DAU Urine 1,2,3 DOF Oral fluid OF1, OF2, OF3 Outdoor activity toys TOYTEST EN71-8 paper exercise 5 p,p'-ddd AQUACHECK Groundwater 7A * Not included in our scope of accreditation **See current application form and scheme description 110 Tel: +44 (0) Fax: +44 (0)

111 Analyte Scheme Matrix **Sample p,p'-ddd (cont.) AQUACHECK 19A p,p'-dde AQUACHECK Groundwater 7A 19A p,p'-ddt AQUACHECK Groundwater 7A 19A Paracetamol TOX Human serum BLD Human blood SM Parathion-ethyl AQUACHECK Groundwater 9 21 Parathion-methyl AQUACHECK Groundwater 9 21 Paroxetine TDM New born calf serum PS21 Particulate contamination by filtration OIL #2 Diesel fuel 2* Partly unmodified grains MAPS Brewers and distillers malt 1,1 Patulin QBS Apple juice 520 PCB (28) AQUACHECK Groundwater 7D 19D CONTEST Soil Group C, 3c Standard solution Group C, 11 PCB (52) AQUACHECK Groundwater 7D 19D CONTEST Soil Group C, 3c Standard solution Group C, 11 PCB (101) AQUACHECK Groundwater 7D 19D CONTEST Soil Group C, 3c Standard solution Group C, 11 PCB (118) AQUACHECK Groundwater 7D 19D CONTEST Soil Group C, 3c Standard solution Group C, 11 PCB (138) AQUACHECK Groundwater 7D 19D CONTEST Soil Group C, 3c Standard solution Group C, 11 PCB (153) AQUACHECK Groundwater 7D 19D CONTEST Soil Group C, 3c Standard solution Group C, 11 PCB (180) AQUACHECK Groundwater 7D 19D CONTEST Soil Group C, 3c Standard solution Group C, 11 Pendimethalin AQUACHECK Groundwater 7A* 19A* Pentachlorobenzene AQUACHECK Groundwater 7A 19A Pentachlorophenol AQUACHECK Groundwater 6B 18B Pepsin protein digestibility (PPD) AFPS Animal feed 1 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 111

112 Analyte Scheme Matrix **Sample Perampanel TDM Human serum AE4* Permanganate index (PI) AQUACHECK Hard water 2H Soft water 2S Peroxide value QFCS Oil or fat 778* Perphenazine TDM New born calf serum PS16 Perylene AQUACHECK Groundwater 7C 19C Pesticides QFCS Fruit or vegetable 773 Dried tea 777* PFOA AQUACHECK Spiking solution 26 PFOS AQUACHECK Spiking solution 26 ph BAPS Ale (Bitter) 1B Lager 1L COSMETICS Liquid cosmetics 23 CONTEST Soil Group B, 3b Soil Group D, 18 DAPS Fermented wort Group A, A1 Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Simulated spirit Group B, B4 Ciders Group C, C1 White or Rosé wine Group D, D1 Red wine Group D, D2 Ready to drink Group E, E1 Liqueur Group E, E2 Cream liqueur Group E, E3 Simulated liqueur Group E, E4 PHARMASSURE Buffer solution 1A QBS Fruit juice 510 Carbonated drinks 515 Dilutable and ready to drink materials 517 QDCS Milk 32A Buffer solution 32B Butter 36 Hard cheese 37 Whole protein concentrate 43 Soft cheese 59 QMAS Dried or cured meat 730 Precooked,raw or processed meat 731 Fish 734 SUPS Molasses 5 MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 ph at C AQUACHECK Higher salinity potable water 1A Hard water 2H Soft water 2S Agricultural soil 14 Sewage sludge 16 Wastewater 17A * Not included in our scope of accreditation **See current application form and scheme description 112 Tel: +44 (0) Fax: +44 (0)

113 Analyte Scheme Matrix **Sample ph at C (cont.) BAPS Brewing liquor 6A* ph at C - High AQUACHECK Poorly buffered waters 2A ph at C - Low AQUACHECK Poorly buffered waters 2A Phenanthrene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Phenobarbitone TDM Human serum TD1 Phenols AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) AQUACHECK 18B CONTEST Soil Group C, 3c Standard solution Phenols (Total) AQUACHECK Industrial wastewater 17B MAPS Brewers and Distillers malt 1,1 21* Group C, 7b Phenol index CONTEST Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 Phenytoin TDM Human serum TD1 Phosphatase QDCS Freeze dried milk 31 (A and B) Phosphate BAPS Lager 2L CONTEST Soil Group D, 18 QFCS Cereal 770 Ready to eat' product 772 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Phosphorus AFPS Animal feed 2 QBS Fruit juice 518 Soft drink 519 QDCS Whole milk powder 42 Phosphorus (Total) AQUACHECK Hard water 1H Soft water 1S Spiking solution 10 Sewage sludge 13 Agricultural soil 14 Industrial wastewater Phthalates TOYTEST Standard solution and plastic material 10 Pirimicarb AQUACHECK Groundwater 8B* 17D 20B* Polarisation SUPS Cane or beet sugar 3 Raw sugar 7 Polymeric triglycerides AFPS Oil or fat 3 Poly-unsaturates QMAS Meat 733 Ponceau 4R (E124) QFCS Liquid 762 Potassium AFPS Animal feed 2 AQUACHECK Hard water 1H Soft water BAPS Lager 2L CONTEST Soil Group D, 18 DAPS Scotch whisky Group B, B1 1S * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 113

114 Analyte Scheme Matrix **Sample Potassium (cont.) DAPS Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 QBS Fruit juice 518 Soft drink 519 QDCS Whole milk powder 41 QMAS Precooked, raw or processed meat 731 Potassium (Total) AQUACHECK Sewage sludge 13 Agricultural soil 14 Pour point OIL #2 Diesel fuel 2* Crude oil 4* Engine oil lubricants 5* Predicted spirit yield, (As is) MAPS Malt - IoB wort dependent 1,3 Pregablin TDM Human serum AE1 Present gravity ( Plato) BAPS Ale (Bitter) 1B Lager 1L Present gravity ( Sacc) BAPS Ale (Bitter) 1B Lager 1L Primidone TDM Human serum TD1 Prometryn AQUACHECK Groundwater 8B* 20B* Propachlor AQUACHECK Groundwater 8* 20* Propazine AQUACHECK Groundwater 8B 20B Propetamphos AQUACHECK Groundwater 9* 21* Propylene glycol DAPS Simulated spirit Group B, B4 Simulated liqueur Group E, E4 Propyzamide AQUACHECK Groundwater 8 20 Protein QDCS Milk 28 Hard cheese 37 Whey powder 38B Whole protein concentrate 43 Skimmed milk powder 51 Whole milk powder 52 Yogurt 58 Soft cheese 59 QFCS Cereal 770 'Ready to eat' product 772 Flour 775 Bread or cake 776 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Fish 734 Pseudomonas aeruginosa count COSMETICS Powder 10 Cream 13 Liquid 16 QWAS Potable water 413 Mineral water 420 * Not included in our scope of accreditation **See current application form and scheme description 114 Tel: +44 (0) Fax: +44 (0)

115 Analyte Scheme Matrix **Sample Pseudomonas aeruginosa detection COSMETICS Powder 10 Cream 13 Liquid 16 Pseudomonas species count QMS Oatmeal 26 Skimmed milk powder 26 QMAS Lyophilised meat 746 QWAS Process water 414 Pyrene AQUACHECK Groundwater 7C 19C CONTEST Soil Group C, 3c Standard solution Group C, 5 Qualitative determination in clean water AQUACHECK Clean water 25 Qualitative organics by GCMS AQUACHECK Clean water 22 Qualitative organics by purge and / or trap GCMS AQUACHECK Clean water 22A Quetiapine and norquetiapine TDM New born calf serum PS17 Rebaudioside A, B, C, D, E and F SUPS Stevia 9* Reducing sugars DAPS White or Rosé wine Group D, D1 Red wine SUPS Cane or beet sugar 3 Molasses 5 Raw sugar 7 Reflectance grade SUPS Cane or beet sugar 1 Refractive index BAPS Ale (Bitter) 1B Lager PHARMASSURE Sugar solution 1E Reid vapor pressure OIL Crude oil 4* Reject fraction (EBC fraction IV plus foreign matter) MAPS Brewers and distillers malt 1,1 Residual fermentable sugars (Total amount of glucose, maltose and maltotriose present) Barley 2 Group D, D2 DAPS Fermented wort Group A, A1 Simulated wort 1L Group A, A2 Residual gravity DAPS Fermented wort Group A, A1 Simulated wort Residual sulfur dioxide MAPS Brewers and distillers malt 1,1 Group A, A2 Residue DAPS Liqueur Group E, E2 Cream liqueur Simulated liqueur Respirable grade quartz AIR Quartz PVC filter 25mm diameter (Workplace air) Retigabine (Ezogabine) TDM Human serum AE3* Risperidone and HO-risperidone TDM New born calf serum PS10 Rubusoside SUPS Stevia 9* Rufinamide TDM Human serum AE2 Saccharin (As free imide) QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 Saccharin (E954) QFCS Liquid 761 Salicylic acid TOX Human serum BLD Human blood Salinity AQUACHECK Wastewater 17A Salmonella serology QMIS Lyophilised test material 102 Salmonella species count QMS Skimmed milk powder 3 Salmonella species detection AFPS Animal feed 6 Group E, E3 Group E, E4 2* SM * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 115

116 Analyte Scheme Matrix **Sample Salmonella species detection (cont.) QCS Grated chocolate 710,711,712 Cocoa powder 714 QGS Gelatine hydrolysate 601 QMAS Lyophilised meat 736 Lyophilised fish or shellfish 740 QMS Oatmeal 6 Skimmed milk powder 6 Tea 28 Herb 30 Spice 31 QWAS Effluent sludge 416 Bathing, surface and wastewater 419 Sea water 422 Salt OIL Crude oil 4* QDCS Butter 36 Hard cheese 37 Soft cheese 59 QFCS Cereal 770 'Ready to eat' product 772 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Fish 734 Saponification number OIL Engine oil lubricants 5* QFCS Oil or fat 778* Saturates QMAS Meat 733 Scorched particles QDCS Skimmed milk powder 38A Whey powder 38B Sediment OIL Crude oil 4* Sediment (Insoluble) SUPS Cane or beet sugar 4 Selenium AFPS Animal feed 2 AQUACHECK Natural water 5 Natural water Groundwater 5A 5G 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1b Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 QFCS Dried tea 780* TOYTEST EN71-3 Standard solution and real material Semi volatile organic compounds CONTEST Standard solution Group C, 20 Sertraline and norsertraline TDM New born calf serum PS08 Settleable solids AQUACHECK Settleable solids 15 3 * Not included in our scope of accreditation **See current application form and scheme description 116 Tel: +44 (0) Fax: +44 (0)

117 Analyte Scheme Matrix **Sample Settled chemical oxygen demand (COD) AQUACHECK Wastewater 17A Shear stability OIL Engine oil lubricants 5* Shigella species detection QMS Oatmeal 12 Sieving test <2.20mm MAPS Brewers and distillers malt 1,1 Barley 2 Sieving test <2.25mm MAPS Barley 2 Sieving test >2.50mm MAPS Barley 2 Sieving test >2.80mm MAPS Brewers and distillers malt 1,1 Barley 2 Sieving test mm MAPS Brewers and distillers malt 1,1 Barley 2 Sieving test mm MAPS Brewers and distillers malt 1,1 Barley 2 Silicate AQUACHECK Hard water 2H Soft water 2S Spiking solution 10 Silver AQUACHECK Natural water 4 Groundwater 4G Industrial wastewater 17C Simazine AQUACHECK Groundwater 8B 20B Smoke point OIL Aviation turbine fuel (Jet A) 3* Sodium AFPS Animal feed 2 AQUACHECK Hard water 1H Soft water 1S Higher salinity potable water 1A BAPS Lager 2L Brewing liquor 6A* CONTEST Soil Group D, 18 DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 QBS Fruit juice 518 Soft drink 519 QDCS Whole milk powder 41 QFCS Cereal 770 'Ready to eat' product 772 Bread or cake 776 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Sodium bicarbonate (Concentration) PHARMASSURE Standard solution 1C Sodium chloride (Concentration) PHARMASSURE Standard solution 1C Soluble extract 0.2mm MAPS Malt - IoB wort dependent 1,3 Soluble extract 0.7mm MAPS Malt - IoB wort dependent 1,3 Soluble extract 1.0mm MAPS Malt - IoB wort dependent 1,3 Soluble extract difference ( mm) MAPS Malt - IoB wort dependent 1,3 Soluble extract difference ( mm) MAPS Malt - IoB wort dependent 1,3 Soluble nitrogen ratio (SNR) MAPS Malt - IoB wort dependent 1,3 Soluble reactive phosphorus AQUACHECK Hard water 2H Soft water 2S Spiking solution 10 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 117

118 Analyte Scheme Matrix **Sample Soluble reactive phosphorus (cont.) AQUACHECK Industrial wastewater 17D Sorbic acid DAPS White or Rosé wine Group D, D1 Red wine Ready to drink Sorbic acid (As free acid) QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 Sorbic acid (E200 - E203) QFCS Liquid 760 Specific gravity COSMETICS Liquid cosmetics 23 Group D, D2 Group E, E1 DAPS Ciders Group C, C1 White or Rosé wine Red wine Ready to drink Liqueur Cream liqueur Simulated liqueur Staphylococcus aureus count COSMETICS Powder 10 Cream 13 Liquid 16 PHARMASSURE Lyophilised test material 4 Staphylococcus aureus detection COSMETICS Powder 10 Cream 13 Liquid 16 PHARMASSURE Lyophilised test material 4 Starch AFPS Animal feed 1 SUPS Raw sugar 7 Sterility PHARMASSURE Lyophilised test material 5 Steviolbioside SUPS Stevia 9* Stevioside SUPS Stevia 9* Steviol glycosides (Total) SUPS Stevia 9* Stimulant drugs QUARTZ Blood 1 Blood and case study 2 Strontium AQUACHECK Natural water 4 TOYTEST Groundwater EN71-3 Standard solution and real material Styrene AQUACHECK Groundwater 6C Group D, D1 Group D, D2 Group E, E1 Group E, E2 Group E, E3 Group E, E4 4G 3 18C Sucralose QBS Carbonated drinks (degassed) 516 Sucrose DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Simulated spirit Group B, B4 QBS Carbonated drinks 515 Dilutable and ready to drink materials 517 SUPS Molasses 5 Sugars AFPS Animal feed 1 Sugars (Total) DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Simulated spirit Group B, B4 * Not included in our scope of accreditation **See current application form and scheme description 118 Tel: +44 (0) Fax: +44 (0)

119 Analyte Scheme Matrix **Sample Sugars (Total) (cont.) DAPS Ready to drink Group E, E1 QCS Chocolate 715 QFCS Cereal 770 Ready to eat product 772 QMAS Dried or cured meat 730 Precooked, raw or processed meat 731 Sulfate AQUACHECK Hard water 1H Soft water 1S Higher salinity potable water 1A Industrial wastewater 17B BAPS Lager 2L Brewing liquor 6A* CONTEST Soil Group D, 18 Soil Group E, 24* Solid waste Group E, 21 Sulfate (Total) CONTEST Soil Group B, 3b Standard solution Group B, 8 Sulfide (Total) AQUACHECK Wastewater 32* Sulfur OIL Crude oil 4* Sulfur content OIL Ultra low sulfur diesel fuel 1* #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Engine oil lubricants 5* Sulfur dioxide BAPS Ale (Bitter) 1B Lager 1L SUPS Cane or beet sugar 3 Sulfur dioxide (Determined as sulfate) AIR Impinger solution (Stack emissions) 34 Sulfur dioxide (E220 - E228) QFCS Liquid 760 Sulfur dioxide (Free) DAPS White or Rosé wine Group D, D1 Red wine Group D, D2 QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 Sulfur dioxide (Total) DAPS Ciders Group C, C1 White or Rosé wine Group D, D1 Red wine Group D, D2 QBS Carbonated drinks (degassed) 516 Dilutable and ready to drink materials 517 Sulphite-reducing bacteria count QGS Gelatine hydrolysate 603 Sulphite-reducing clostridia count QMAS Lyophilised meat 738 QWAS Potable water 413 Bathing, recreational and surface water 421 Sulphite-reducing clostridia spores count ONLY QWAS Potable water 413 Sulphite-reducing clostridia spores detection ONLY QWAS Mineral water 424 Sulphite-reducing clostridia detection QWAS Potable water 413 Mineral water 424 Sunset yellow (E110) QFCS Liquid 762 Suspended solids AQUACHECK Spiking solution 3 Spiking solution 11 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 119

120 Analyte Scheme Matrix **Sample Suspended solids (cont.) AQUACHECK Wastewater 17A BAPS Effluent simulant solution 6B* Tablet testing: diameter, disintegration, dissolution, friability, hardness, resistance to crushing, thickness, weight etc... PHARMASSURE Tablet 2C* Tartrazine (E102) QFCS Liquid 763 Taste - Alcoholic / Solvent BAPS Lager / ale (Bitter) 5 Taste - Astringent BAPS Lager / ale (Bitter) 5 Taste - Bitter BAPS Lager / ale (Bitter) 5 Taste - Body BAPS Lager / ale (Bitter) 5 Taste - Burnt BAPS Lager / ale (Bitter) 5 Taste - Caramel BAPS Lager / ale (Bitter) 5 Taste - Cereal BAPS Lager / ale (Bitter) 5 Taste - DMS BAPS Lager / ale (Bitter) 5 Taste - Fruity / Citrus BAPS Lager / ale (Bitter) 5 Taste - Fruity / Estery BAPS Lager / ale (Bitter) 5 Taste - Hop BAPS Lager / ale (Bitter) 5 Taste - Linger BAPS Lager / ale (Bitter) 5 Taste - Malty BAPS Lager / ale (Bitter) 5 Taste - Other BAPS Lager / ale (Bitter) 5 Taste - Other sulfur BAPS Lager / ale (Bitter) 5 Taste - Oxidised / Aged BAPS Lager / ale (Bitter) 5 Taste - Sour BAPS Lager / ale (Bitter) 5 Taste - Sweet BAPS Lager / ale (Bitter) 5 Tablet testing PHARMASSURE Sample format varies for each round 2C* TD-Amikacin TDM Human serum TD1 TD-Gentamicin TDM Human serum TD1 TD-Tobramycin TDM Human serum TD1 TD-Vancomycin TDM Human serum TD1 Teicoplanin TDM Human serum AT07 Tellurium AQUACHECK 12* Terbutryn AQUACHECK Groundwater 8B 20B* Terpinene-4-ol DAPS Clear distilled spirit Group B, B3 Tetrachloroethene AIR Charcoal filled glass sorbent tubes (Workplace air) 6* AQUACHECK Groundwater 6A Groundwater Tetrachloromethane CONTEST Standard solution Group C, 19 7B 18A 19B Soil Group C, 25* Tetra-iso-alpha-acids BAPS Lager 2L Ale 3 Thallium AIR Metals impinger solution (Stack emissions) 33 Metals and fly ash on Quartz filter 47mm diameter (Stack emissions) CONTEST Standard solution Group A, 1a 38* Soil extract Group A, 2 Theobromine QCS Chocolate 715 Soil Group A, 3a * Not included in our scope of accreditation **See current application form and scheme description 120 Tel: +44 (0) Fax: +44 (0)

121 Analyte Scheme Matrix **Sample Theobromine (cont.) QCS Cocoa powder 716 Theophylline TDM Human serum TD1 Thermal stability OIL Aviation turbine fuel (Jet A) 3* Thermophilic acidophilic bacteria count SUPS Lyophilised test material 8 Thermophilic aerobic spores count QMS Oatmeal 11 Skimmed milk powder 11 Thermotolerant coliforms count QMS Oatmeal 20 Skimmed milk powder 20 Thiocyanate CONTEST Soil Group B, 3b Standard solution Group B, 16 Soil Group D, 18 Thioridazine TDM New born calf serum PS13 Thousand corn weight MAPS Barley 2 Tiagabine TDM Human serum AE1 Tin AQUACHECK Natural water 5 Natural water Groundwater Industrial wastewater 5A 5G 17C CONTEST Standard solution Group A, 1a TOYTEST Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 EN71-3 Standard solution and real material QBS Fruit juice 518 Soft drink 519 Titratable acidity QDCS HCl solution 29 Whipping cream 39 Skimmed milk powder 51 Whole milk powder 52 Single cream 53 Double cream 54 Milk 56 Tobramycin TDM Human serum AT02 Titre PHARMASSURE Standard solution 1C 3 Toluene AIR Charcoal filled glass sorbent tubes (Workplace air) Tenax TA filled sorbent tubes (Workplace air) Tenax TA filled sorbent tubes (Workplace air) Tenax TA filled sorbent tubes (Workplace air) 5* 7* 12* 21* AQUACHECK Groundwater 6C 18C CONTEST Standard solution Group C, 15 Soil 22* Topiramate TDM Human serum AE2 Total acidity DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 121

122 Analyte Scheme Matrix **Sample Total acidity (cont.) DAPS Ciders Group C, C1 White or Rosé wine Group D, D1 Red wine Group D, D2 Ready to drink Group E, E1 Liqueur Group E, E2 Total aerobic count QWAS Process water 414 Total aerobic count at 22 C QWAS Potable water 412 Mineral water 420 Total aerobic count at 37 C QWAS Potable water 412 Mineral water 420 Total aerobic bacterial count (Spread) BAPS Lyophilised test material 4 H Lyophilised test material 4 L Total aerobic microbial count (Pour) BAPS Lyophilised test material 4 H Lyophilised test material 4 L Total aerobic mesophilic count PHARMASSURE Lyophilised test material 4 QBS Fruit juice 500 Soft drink 501 QCS Cocoa powder 713 Chocolate 717* QGS Gelatine hydrolysate 602 QMAS Lyophilised meat 735 Lyophilised fish or shellfish 739 QMS Oatmeal 16 Skimmed milk powder 16 Tea 29 SUPS Lyophilised test material 6 Total aerobic mesophilic count (Filtration) QBS Lyophilised test material 505 Total anaerobic mesophilic count QMS Oatmeal 15 Skimmed milk powder 15 Total anaerobic microbial count BAPS Lyophilised test material 4 H Lyophilised test material 4 L Total bacterial count PHARMASSURE Lyophilised test material 4 Total coliforms count QWAS Bathing, surface and wastewater 419 Sea water 422 Total dietary fibre QFCS Cereal 770 'Ready to eat' product 772 Bread or cake 776 Total dissolved solids CONTEST Soil Group E, 24* Solid waste Group E, 21 Total dissolved solids (180 C) AQUACHECK Higher salinity potable water 1A Hard water 2H Soft water 2S Wastewater 17A Total dimethyl sulfide (DMS) MAPS Brewers and distillers malt 1,1 Total gas pressure BAPS Ale (Bitter) 1B Lager 1L Total hardness AQUACHECK Hard water 1H Soft water 1S Total hydrocarbons by GC analysis AQUACHECK Mineral oil in water 23 Total hydrocarbons by Gravimetric analysis AQUACHECK Mineral oil in water 23 * Not included in our scope of accreditation **See current application form and scheme description 122 Tel: +44 (0) Fax: +44 (0)

123 Analyte Scheme Matrix **Sample Total hydrocarbons by IR analysis AQUACHECK Mineral oil in water 23 Total monohydric phenols CONTEST Standard solution Group C, 7b Total monohydric phenols (By distillation) CONTEST Standard solution Group C, 7a Total monosubstituted methylphenols AQUACHECK Groundwater 6B* 18B* Total oil and grease AQUACHECK Oil and grease in water 24 Total organic carbon (TOC) AQUACHECK Higher salinity potable water 1A CONTEST Soil Group C, 3c Total organic carbon / dissolved organic carbon (TOC / DOC) CONTEST Soil Group D, 18 Total oxidised nitrogen (TON) AQUACHECK Hard water 2H Soft water 2S Spiking solution 10 Total PAH CONTEST Soil Group C, 3c Total phenols (Sum of phenol, cresols and xylenols) CONTEST Soil Group C, 7b Total TPH (C 10 -C 40 inclusive) CONTEST Soil Group C, 3c TPH (C 10 -C 40 inclusive) CONTEST Standard solution Group C, 14a TPH, Aliphatic C 6 CONTEST Soil Group C, 23* Standard solution Group C, 14b TPH, Aliphatic >C 6 -C 8 CONTEST Soil Group C, 23* Standard solution Group C, 14b TPH, Aliphatic >C 8 -C 10 CONTEST Soil Group C, 23* Standard solution Group C, 14b TPH Aliphatic >C 10 -C 12 CONTEST Soil Group C, 3c TPH Aliphatic >C 12 -C 16 CONTEST Soil Group C, 3c TPH Aliphatic >C 16 -C 21 CONTEST Soil Group C, 3c TPH Aliphatic >C 21 -C 35 CONTEST Soil Group C, 3c TPH Aliphatic >C 35 -C 40 CONTEST Soil Group C, 3c TPH, Aromatic C 6 CONTEST Soil Group C, 23* Standard solution Group C, 14b TPH, Aromatic >C 6 -C 8 CONTEST Soil Group C, 23* Standard solution Group C, 14b TPH, Aromatic >C 8 -C 10 CONTEST Soil Group C, 23* Standard solution Group C, 14b TPH Aromatic >EC 10 -EC 12 CONTEST Soil Group C, 3c TPH Aromatic >EC 12 -EC 16 CONTEST Soil Group C, 3c TPH Aromatic >EC 16 -EC 21 CONTEST Soil Group C, 3c TPH Aromatic >EC 21 -EC 35 CONTEST Soil Group C, 3c TPH Aromatic >EC 35 -EC 40 CONTEST Soil Group C, 3c Total polyphenols BAPS Lager 2L Total solids AIR Dust rinsing solution (Stack emissions) 39* AQUACHECK Agricultural soil 14 QDCS Milk 28 QDCS Yogurt 58 Total solids (105±5)ºC) AQUACHECK Sewage sludge 16 Total soluble nitrogen (TSN) BAPS Lager 2L MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 Total trans fatty acids QMAS Meat 733 Total viable count AFPS Animal feed 7 Total volatile nitrogen (TVN) QMAS Fish 748* * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 123

124 Analyte Scheme Matrix **Sample Toxicology case studies TOX Serum CAS Toxicology in blood TOX Human blood QT Tramadol QUARTZ Blood 3 Trazodone TDM New born calf serum PS29 Trichlopyr AQUACHECK Groundwater 8 Trichloroethene AIR Charcoal filled glass sorbent tubes (Workplace air) Urine AQUACHECK Groundwater 6A Groundwater CAS CONTEST Soil Group C, 25* 6* 7B 18A 19B Standard solution Group C, 19 Trichloromethane CONTEST Soil Group C, 25* Standard solution Group C, 19 Triclopyr AQUACHECK 20 Trietazine AQUACHECK Groundwater 8B* 20B* Trifluralin AQUACHECK Groundwater 7A Trimethylamine (TMA) QMAS Fish 748* Trimipramine and nortrimipramine TDM New born calf serum PS09 Turbidity AQUACHECK Spiking solution 3 Unknown microorganism (biochemical, microscopical and indentification tests) 19A Spiking solution 11 SUPS Cane or beet sugar 1 QMIS Lyophilised test material 101 Unsaponifiable matter AFPS Oil or fat 3 QFCS Oil or fat 778* Uranium AQUACHECK 12* Valproate TDM Human serum TD1 Vanadium AIR Metals impinger solution (Stack emissions) Metals and fly ash on Quartz filter 47mm diameter (Stack emissions) 33 38* AQUACHECK Natural water 5 Natural water 5B Groundwater 5G Sewage sludge 13 Agricultural soil 14 Industrial wastewater 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a VDK as diacetyl BAPS Lager 2L High bitterness and / or colour material 3 Venlafaxine and norvenlafaxine TDM New born calf serum PS20 Vibrio fischeri 30 minute IC50 (ISO ) AQUACHECK Ecotoxicology 50 Vibrio parahaemolyticus detection QMS Oatmeal 13 Vibrio species detection QMS Oatmeal 13 * Not included in our scope of accreditation **See current application form and scheme description 124 Tel: +44 (0) Fax: +44 (0)

125 Analyte Scheme Matrix **Sample Vigabatrin TDM Human serum AE2 Vinyl chloride CONTEST Soil Group C, 25* Standard solution Group C, 19 Viscosity COSMETICS Liquid cosmetics 23 MAPS Malt - EBC wort dependent 1,2 Malt - IoB wort dependent 1,3 Viscosity (Kinematic) OIL #2 Diesel fuel 2* Aviation turbine fuel (Jet A) 3* Crude oil 4* Engine oil lubricants 5* Viscosity (Low temperature) OIL Engine oil lubricants 5* Viscosity (Tapered bearing) OIL Engine oil lubricants 5* Viscosity (Tapered plug) OIL Engine oil lubricants 5* Vitamin A, C, D QDCS Milk powder 44 Vitamin A (as Retinol) QFCS Cereal 770 Vitamin B1 (Thiamine) QFCS Cereal 770 Bread or cake 776 Vitamin B2 (Riboflavin) QFCS Cereal 770 Bread or cake 776 Vitamin B3 (Niacin) QFCS Cereal 770 Bread or cake 776 Vitamin B3 (Nicotinamide) QBS Soft drink 521* Vitamin B5 (Pantothenic acid) QBS Soft drink 521* Vitamin B6 (Pyridoxine) QBS Cereal 770 QFCS Soft drink 521* Vitamin B9 (Folic acid) QFCS Cereal 770 Vitamin B12 (Cobalamins) QFCS Cereal 770 Vitamin B12 (Cyanocobalamin) QBS Soft drink 521* Vitamin C (Ascorbic acid) QBS Cereal 770 QFCS Soft drink 521* Vitamin D QFCS Cereal 770 Vitamin 5 (Pantothenic acid) QFCS Cereal 770 Volatility OIL Engine oil lubricants 5* Volatile acidity DAPS Scotch whisky Group B, B1 Dark distilled spirit Group B, B2 Clear distilled spirit Group B, B3 Ciders Group C, C1 White or Rosé wine Group D, D1 Red wine Group D, D2 Ready to drink Group E, E1 Liqueur Group E, E2 Volume AIR Impinger solution (Stack emissions) 33 Impinger solution (Stack emissions) 34 Impinger solution (Stack emissions) 35 Impinger solution (Stack emissions) 36 Impinger solution (Stack emissions) 37 Volume of sample provided AQUACHECK Mineral oil in wastewater 23 Oil and grease in wastewater 24 Water QFCS Oil or fat 778* OIL Crude oil 4* * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 125

126 Analyte Scheme Matrix **Sample Water (cont.) OIL Engine oil lubricants 5* Water activity QFCS Cereal 774 Cured meat 774 Hard cheese 774 Water content OIL Engine oil lubricants 5* Water extractable boron AQUACHECK Agricultural soil 14 Water and sediment OIL #2 Diesel fuel 2* Water separation OIL Aviation turbine fuel (Jet A) 3* Water soluble boron CONTEST Soil Group B, 3b Standard solution Group B, 10 Water soluble chloride CONTEST Soil Group B, 3b Water soluble fluoride CONTEST Soil Group B, 3b Water soluble sulfate CONTEST Soil Group B, 3b Wild yeast count BAPS Lyophilised test material 4 H Lyophilised test material WPNI QDCS Skimmed milk powder 51 4 L Whole milk powder 52 Xylene (All isomers) AIR Charcoal filled glass sorbent tubes (Workplace air) 5* Tenax TA filled sorbent tubes (Workplace air) Tenax TA filled sorbent tubes (Ambient air) 7* 12* o-xylene AQUACHECK Groundwater 6C 18C CONTEST Standard solution Group C, 15 Soil Group C, 22* m + p-xylenes AQUACHECK Groundwater 6C 18C CONTEST Standard solution Group C, 15 Soil Group C, 22* p-xylene AIR Tenax TA filled sorbent tubes (Indoor or Chamber air) AQUACHECK Groundwater 6C Xylene (Total) AQUACHECK Groundwater 6C Xylenols CONTEST Soil Group C, 3c Standard solution 21* 18C 18C Group C, 7b Xylenols (Total) CONTEST Standard solution Group C, 7b Yeast count AFPS Animal feed 7 PHARMASSURE Lyophilised test material 4 QBS Fruit juice 500 Soft drink 501 QCS Cocoa powder 713 Chocolate 717* QMAS Lyophilised meat 746 QMS Oatmeal 23 Skimmed milk powder 23 QWAS Process water 414 SUPS Lyophilised material 6 * Not included in our scope of accreditation **See current application form and scheme description 126 Tel: +44 (0) Fax: +44 (0)

127 Analyte Scheme Matrix **Sample Yeast and mould (Total count) COSMETICS Powder 10 Cream 13 Liquid 16 PHARMASSURE Lyophilised test material 4 QWAS Process water 414 QMS Oatmeal 23 Skimmed milk powder 23 Tea 29 Yeast count (Filtration) QBS Lyophilised test material 505 Yersinia enterocolitica detection QMS Skimmed milk powder 14 Zinc AFPS Animal feed 2 AIR Cellulose filter (Workplace air) 1* AQUACHECK Natural water 4 Groundwater Natural water 4G 5B 12 Sewage sludge 13 Agricultural soil 14 Industrial wastewater BAPS Lager 2L 17C CONTEST Standard solution Group A, 1a Soil extract Group A, 2 Soil Group A, 3a Soil Group D, 18 Solid waste Group E, 21 QDCS Whole milk powder 42 TOYTEST EN71-3 Standard solution and real material Zonisamide TDM Human serum AE2 Zuclopenthixol TDM New born calf serum PS23 3 * Not included in our scope of accreditation **See current application form and scheme description [email protected] Website: 127

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