2012 CCR Mouse Research Models. Laboratory Animal Sciences Program CORE Resources

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1 National Cancer Institute 2012 CCR Mouse Research Models Mouse 101 Laboratory Animal Sciences Program CORE Resources Lionel Feigenbaum, Ph.D. Laboratory Animal Sciences Program May 7, 2012

2 CORE Resources Centralized scientific resources have been established to provide investigators access to new technologies and to complement and advance NCI s research efforts.

3 Criteria Institutional commitment High demand Current and developing technologies Time savings Cost effective

4 LASP CORE Programs Mouse Model Development Animal Care Strain Preservation In vivo Imaging Phenotyping-PHL NCI-Repository

5 Laboratory Animal Sciences Program Animal Program Management LASP Frederick and Bethesda Facilities Comprehensive management of animal facilities and operations Animal holding, husbandry, and veterinary care Animal technical services comprising experimental procedures and colony management Quarantine of imported animals Rederivation of pathogen-carrying strains Diagnostic testing services to maintain the health status and monitor genetic integrity of animal research colonies Management of 24 rodent and nonhuman primate research facilities Support of 251 principal investigators encompassing 630 active Animal Study Proposals Maintenance of 125,535 animals occupying 48,034 cages Facility space: 157,548 square feet

6 Mouse Model Development Transgenic mice Generation of transgenic mice by pronuclear microinjection with: Plasmid Cosmid Bacterial artificial chromosome (BAC) P1 artificial chromosome (PAC) Lentiviral vectors Contact: Inject foreign DNA into male pronucleus Gene targeting Production of: Knockout (KO) Knockin (KI) Conditional KO and KI Contact: Selection of recombinants Gene targeting by homologous recombination Screening for targeting events neo r HS V-tk Gene X WT ES 1 ES 2 Cryopreservation and assisted reproduction Colony expansion of genetically engineered mice (GEM) for large-scale studies Contact: ostermeiergc@mail.nih.gov Spermatozoa for in vitro fertilization Xenograft model development Orthotopic Heterotopic Contact: feigenbl@mail.nih.gov

7 Small Animal Imaging Program (SAIP) at Frederick National Laboratory Provide state-of-the-art in vivo imaging to the NCI community. Ability to understand and develop new mouse models, molecular markers, and theragnostics, and monitor tumors in vivo.

8 3T MRI (Philips Medical Systems) Normal Inflammation Polyps Detected Polyps Enlarge NUCLEAR IMAGING NanoSPECT/CT (Bioscan, Inc.) OPTICAL IMAGING Bioluminescence Xenogen IVIS SPECTRUM (Caliper Life Sciences) Untreated Days after 2% DSS treatment Imaging colon tumors. ULTRASOUND IMAGING (VisualSonics, Inc.) Imaging angiogenesis using microbubbles tagged with VEGFR2 antibodies. Fused SPECT/CT Inveon PET/CT (Siemens Medical) Cell trafficking Fluorescence Maestro-GNIR (Cambridge Research Institute) OPTIX MX2 (Advanced Research Technologies) Data Analysis Biodistribution Anatomical and Physiological Volume Image Probe Uptake (Wash-In/Out) Parametric Maps Kinetic Modeling Fluorescence Lifetime Tumor Vasculature Blood Flow Tumor imaging using [18F]-deoxyglucose, a glucose analog, to image metabolic activity. Contact: kalenj@mail.nih.gov

9 Pathology/Histotechnology Lab (PHL) Service core that integrates anatomic pathology, molecular histology, and imaging Works with other LASP cores to increase capabilities Functional units: Anatomic Pathology (Contact: Histotechnology (Contact: Necropsy, Tissue Processing, Hematology, Clinical Chemistry Microtomy, Staining Molecular Histology (IHC, ISH) Nucleic Acid Isolation/LCM Image Capture and Analysis

10 Histotechnology Services Animal Necropsy Full Bioassay Embryo Microdissection Tissue Processing Trim Tissue Blocks Paraffin, Frozen, Plastic Microtomy/Vibratome Histological Staining Tissue Derivatives DNA/RNA/Protein LCM (Laser Capture) Pathology Driven

11 Hematology and Clinical Chemistry

12 Immunohistochemistry PHL IHC Database (400+ Protocols) Antigen is recognized by a specific Antibody Uses in veterinary pathology Confirm diagnosis Detect micrometastasis Prognostic markers Diagnosis of infectious disease

13 In Situ Hybridization (ISH) Identifying the cellular source of a protein using mrna as a proxy Method that utilizes a labeled anti-sense riboprobe to visualize corresponding target mrna Custom Probe Design, Synthesis and Validation Optimized Tissue Processing RNAse-Free Microtomy Novel Assay Development Control Reagents Intavis ProVs Robot Stainer Digital Image Documentation Pathology Interpretation

14 Digital Image Capture Digital Whole Slide Scanning (Aperio ScanScope) Publication-ready image files at 5 40x native resolution Digital Slide Conferencing ( telepathology ): Secure web-access to images and/or annotation prior to LCM

15 Laser Capture Microdissection and Laser Cutting Workflow: Workflow: Sample Preparation Sample Preparation Pathology Pathology Review Review (A) (A) LCM LCM Selection Selection (B) (B) Capture Capture (C) (C) Validation (D) Validation (D) A B C D

16 Nucleic Acid Isolation The Autogen 245 nucleic acid workstation automates and standardizes isolation of DNA and/or RNA from 1 24 fresh, frozen, or fixed samples AutoGen 245 Workstation Homogenization and inactivation of nucleases occur simultaneously. Acid phenol extraction compatible with microrna studies. Yield and purity check via spectrophotometer (NanoDrop). h

17 Nucleic Acid Isolation The Agilent 2100 Bioanalyzer capillary electrophoresis system simplifies quality analysis of RNA Agilent 2100 Bioanalyzer Analysis via NanoChip or PicoChip results in an accepted measure of RNA quality termed the RNA Integrity Number (RIN). Material produced is suitable for CGH, sequencing, global gene expression profiling, qpcr, etc. h

18 Veterinary Pathology Study design Protocol development Data analysis Animal imaging validation

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23 Obtaining Genetically Engineered Mice Generation: Institutional core facilities Commercial production facility Individual laboratories Obtain already generated models: Mutant mouse repositories-imr, MMRRC, EMMA, NCI Individual laboratories

24 The NCI Repository Resource generated in support of the NCI s Mouse Models of Human Cancers Consortium (MMHCC). Mission: Preservation, propagation, and distribution of mouse models for cancer research. Current Repository holdings 132 strains being distributed AMDL performs genetic quality control for the MMHCC Repository.

25 Useful Website Links Laboratory Animal Sciences Program NCI Repository International Mouse Strain Resources The IMSR is a searchable online database of mouse strains and stocks available worldwide, including inbred, mutant, and genetically engineered mice. MMRRC-Mouse Mutant Regional Resource Centers