Intelligent Polymers. SPE cartridges and associated products

Transcription

1 Intelligent Polymers SPE cartridges and associated products Q1 v2 2013

2 Dear customers We are pleased to present you our catalogue containing innovative products to help you to achieve your goals. This catalogue is dedicated to meet analytical chemists needs for sample preparation, sample clean-up, extraction and purification. At POLYINTELL, Our R&D team is fully committed to developing quality analytical and diagnostic products that help chemists get accurate information as simply as possible; Our manufacturing organisation supported by highly skilled sales and support teams is at your disposal to satisfy you; Our obsession to manufacture and provide you the high quality products is just partially demonstrated by our ISO 9001: 2008 certification. Your satisfaction is our engine. Kaynoush Naraghi CEO QUALITY POLICY To develop a long term and durable partnership with its customers, POLYINTELL ensures the best quality of its products and services. As an ISO9001:2008 certified company, POLYINTELL has implemented Quality management system requirements to show its commitment to quality, customers, and a willingness to work towards improving efficiency. In addition, to ensure the best quality of its products, the performance is checked by following several QC tests according to each product s quality control procedure. After passing all these tests, the products receive a certificate of analysis which proved the compliance with the defined criterion. TECHNICAL SUPPORT POLYINTELL has fully integrated technologies platform with specialized teams in organic chemistry, polymer chemistry, analytical and bioanalytical chemistry who are at your disposal to guide you in your challenges. At POLYINTELL, we are committed to providing the best technical support possible. Our Technical Support Group is a team of highly qualified M.Sc. and PhD Chemists, who are at your disposal to resolve your problem and to answer to your queries. For technical inquiries, feel free to contact us either by tech.support@polyintell.com, or by phone at: +33 (0) We are also very thankful to customer s feedback about our products, protocols and customer services by to: contact@polyintell.com 2

3 TABLE OF CONTENTS SOLID PHASE EXTRACTION PRODUCT SELECTION GUIDE 5 SORBENTS 6 SOLID PHASE EXTRACTION 7 QUICK LOOK-UP GUIDE 8 AFFINIMIP SPE (MOLECULARLY IMPRINTED POLYMER) 9 AFFINIMIP SPE CONCEPTS 10 AFFINIMIP SPE FOR ANALYTICAL PURPOSE 11 AFFINIMIP SPE VS IMMUNOAFFINITY COMPARATIVE STUDY 12 AFFINIMIP SPE available APPLICATIONS 14 Zearalenone 15 Ochratoxin A 16 Patulin 17 Fumonisins and Zearalenone (simultaneously) 18 Tamoxifen 19 Bisphenol A 20 Protocol Comparison - AFFINIMIP SPE Bisphenol A vs competitors 21 Chloramphenicol 22 GSH 23 Prep GSH Adducts 24 Estrogens 25 Zeranol residues 26 Catecholamines 27 Metanephrines 28 Amphetamines 29 Phenolics 30 AFFINIMIP SPE 18 F FOR RADIOFLUORATION PURIFICATION 31 AFFINIMIP SPE 18 F 32 AFFINIMIP SPE 18 F - Aromatic Nucleophilic Substitution 33 AFFINIMIP SPE 18 F - Nucleophilic Fluoration 34 AFFINIMIP SPE product list 35 AttractSPE TM POLYMERIC CARTRIDGES 37 SELECTION OF THE AttractSPE TM CARTRIDGES 38 AttractSPE W/O - Hydrophilic Lipophilic Balance (HLB) 39 AttractSPE DVB - Reversed Phase 39 AttractSPE WAX - Weak Anion Exchange 40 AttractSPE WCX - Weak Cation Exchange 40 AttractSPE SAX - Strong Anion Exchange 41 AttractSPE SCX - Strong Cation Exchange 41 Polymeric AttractSPE TM product list 42 SiliaPrep SILICA BASED SORBENT SPE CARTRIDGES 43 PRODUCT SELECTION GUIDE BY MANUFACTURER 44 Silica-Based Sorbents Normal phases 45 Silica silica Silica Cyano Silica Diol nec Silica Florisil and Florisil PR Silica Alumina-Acidic, Neutral and Basic 3

4 DETAILED TABLE OF CONTENTS SiliaPrep SILICA BASED SORBENT SPE CARTRIDGES (continued) Silica-Based Sorbents Reversed phases 46 Silica C18 Silica C18 nec Silica C18 WPD Silica C8 and C8 nec Silica Phenyl Silica-Based Sorbents Ion Exchange and Mixed Mode phases 47 Silica SAX (TMA chloride) Silica SAX-2 nec (TMA Acetate) Silica WCX (Carboxylic Acid) Silica WAX (Amine) Silica SCX (Tosic Acid) and SCX-2 (PropylSulfonic Acid) Silica Carbonate Silica C8/Tosic Acid and C8/Propylsulfonic Acid Silica Tosic Acid/TMA chloride SiliaPrep SILICA-BASED SPE CARTRIDGES Product List 48 MYCOTOXINS STANDARDS & SPE ACCESSORIES 52 MYCOTOXINS STANDARDS 53 SPE ACCESSORIES 54 CUSTOM-MADE PRODUCTS & SERVICES 55 PROJECT DEVELOPMENT 57 LIST OF PUBLICATIONS & APPLICATION NOTES 58 ABOUT POLYINTELL 60 ORDERING INFORMATION 60 4

5 SOLID PHASE EXTRACTION PRODUCT SELECTION GUIDE 5

6 SORBENTS Sample preparation and Clean-up are the key steps in trace analysis for analytical chemist. Solid phase extraction (SPE) is a powerful technique to provide a rapid and selective sample clean-up, a high recovery and the concentration necessary for accurate quantitative analysis. A large number of phase chemistries is available and can be processed using dedicated vacuum manifold accessories. A multitude of phase chemistries and formats (SPE tubes, 96-well plates,.) are available in this catalog. We offer a comprehensive range of SPE sorbents to give you all elements to face the increasingly complex and diverse sample preparation challenges to: Simplify data analysis by removing interferences Increase sensitivity and reliability by enrichment of the analyte Obtain high and reproducible recovery yields from complex samples SPE Sorbents As specialist, POLYINTELL offers a complete range of SPE cartridges for solid phase extraction from very specific to a crude clean-up phases, from silica to polymers, from conventional to more sophisticated sorbents. AFFINIMIP SPE for high affinity extraction of analyte from complex matrices and ready to use protocols. AttractSPE TM polymeric cartridges and SiliaPrep silica-based sorbent cartridges for less selective extraction requiring a method development. AFFINIMIP SPE AFFINIMIP SPE is a selective solid phase extraction based on Molecularly Imprinted Polymers (MIP) that combines the advantages of immune-affinity columns regarding the selectivity and those of polymeric SPE sorbents in terms of costs and robustness. Developed for very problematic analyte purification, an instruction sheet gives all required information to successfully carry out the analysis, including the protocol. No method development is required. The protocol is easy, simple and fast. The chromatogram analysis is fast thanks to the affinity between the analyte and AFFINIMIP SPE. Methods are developed for the usual analyte containing matrices. These matrices can be as diverse as aqueous matrices (urine, serum), fully organic matrices (oil), powder (milk) or cereals grains. If your sample preparation is complex or gives inappropriate results, the development of an AFFINIMIP SPE can be a solution. Moreover, POLYINTELL has developed unique products for radiopharmaceutical applications (Catalog for radiotracers purification). AttractSPE TM Polymeric sorbents AttractSPE TM polymeric sorbents are the last generation of SPE sorbent. They are crosslinked polymer particles bearing various chemical functionalities. A limited number of sorbent (6 products) simplifies your choice and covers the full range of analysis using aqueous matrices. These sorbents provide all the advantages of polymeric sorbents, including robustness (to solvent, ph and T), simplified method development, wide applicability and not affected by drying out. The combination of the water-wettable optimised surface chemistry, high surface area and ph stability ensures high reproducible recoveries for a wide range of analytes. For the same application, less sorbent and less solvent are required than for silica-based sorbent and so, their added value is particularly obvious for trace analysis and for small volume sample. Silica-based sorbents The first sorbents historically used for SPE applications, Silica-based sorbents offer a broad range of chemically modified sorbents. This chemistry goes from very polar sorbent (bare silica) to hydrophobic sorbent (endcapped saturated hydrocarbon modified silica) passing through intermediate polarity (for instance, amino modified silica). These sorbents are sensitive to extreme ph. 6

7 SOLID PHASE EXTRACTION SPE PROCEDURE STEPS Solid phase extraction requires the following steps: 1- Sample preparation: This step is necessary to obtain a loading solution compatible with retention condition in the SPE column. In case of solid matrices, this solution must also extract analytes from these matrices. 2- Conditioning: The SPE cartridges are conditioned with the appropriated solvents to fully wet sorbent and enable further interactions between the analytes and the sorbent. 3- Loading: the percolation solution is loaded through the SPE cartridge. The analyte must be retained in the column as well as unwanted compounds. 4- Washing: interferents and unwanted compounds are washed off by using several solvents. 5- Elution: The desired analyte is extracted from the SPE cartridge. Loading Washing Elution Analytes of interest Retention Mechanism SPE cartridges are available in a complete and wide range of sorbents. A sorbent is often classified according to the main retention mechanism between the analyte and the sorbent (normal phase, reversed phase, ionexchange, mixed-mode). Normal phase retention mechanisms are based on polar-polar or dipole-dipole interactions between the analyte and a silica-based sorbent. Reversed phase retention mechanisms are based on non polar- non polar interactions and Van der Waals dispersive forces. The sorbent is hydrophobic (polymeric sorbent or alkyl or phenyl modified silica-based sorbent). Ion-exchange retention mechanism use electrostatic interaction between a charged sorbent and the ionic analyte. The sorbent is charged with the opposite charge of the analyte. A cationic (respectively anionic) analyte interacts with a cation (respectively anion) exchange sorbent. Mixed-mode sorbents mainly interact through reversed phase and ion exchange retention mechanisms. They are available as a polymeric sorbent (AttractSPE TM SAX, WAX, WCX or SCX) or as SiliaPrep. 7

8 QUICK LOOK UP GUIDE The choice of the sorbent is crucial for a successful sample pre-treatment. When AFFINIMIP SPE exists for the analyte, the choice is very simple and the problem is solved. The protocol is ready to use. AFFINIMIP SPE have been developped for very problematic analysis with common SPE sorbents. When no AFFINIMIP SPE product exists, the sorbent chemistry must be chosen very carefully and each step of the protocol must be optimized. This choice is based on the nature of the analyte and of the matrix, on the loading condition and on the planned retention mechanism with the sorbent. The following scheme gives a brief guideline to select the family of sorbents. For specific applications such as trace analysis or complex matrices, conventional SPE sorbents may give very complex analysis or unsatisfaying results. The development of an AFFINIMIP SPE sorbent for this application is a solution. Analyte of interest Existing AFFINIMIP SPE (p9) product NO YES Problem solved Ready to use Protocol Selection of a sorbent Nature of the matrix Aqueous Water miscible organic solvent Not water miscible organic solvent Nature of the analyte ionic Neutral Polar to moderately polar Moderately to Non Polar Attract SPE TM W/O (p39) Normal phase SiliaPrep (p45) Sorbent Ion-Exchange AttractSPE TM (p40) Ion-Exchange SiliaPrep (p47) Reversed phase SiliaPrep (p46) AttractSPE TM DVB (p39) 8

9 AFFINIMIP SPE Selective Solid Phase Extraction Molecularly Imprinted Polymers for the Selective Extraction of Trace Analytes from Complex Matrices Food / Feed Safety Environment Cosmetics Pharmaceutical R&D 9

10 AFFINIMIP SPE - CONCEPTS Selective Solid Phase Extraction Molecularly Imprinted Polymers for the Selective Extraction of Trace Analytes from Complex Matrices New Extraction Phase Based on Molecularly Imprinted Polymers Molecularly imprinted polymer is a polymer with a «memory» of the shape and functional groups of a template molecule. POLYINTELL designs this material in order to recognize selectively the target molecule, even in the presence of compounds with structure and functionality similar to those of the template. Using an imprinting process, we design a material with high affinity for a target molecule: Molecular Recognition materials POLYINTELL designs these materials for an broad variety of molecules, such as natural products (mycotoxines, sugars ), pharmaceuticals, peptides etc Advantages of SPE based molecularly imprinted polymers High affinity and selectivity Stable to ph variation Temperature variation Organic solvents AFFINIMIP Synthetic materials Ensure analysis Reproducibility and reliability via robust and rapid methodology Analytical and preparative applications in research and production 10

11 AFFINIMIP SPE FOR ANALYTICAL PURPOSE AFFINIMIP SPE is a selective solid phase extraction based on Molecularly Imprinted Polymers (MIP) that combines the advantages of immune-affinity columns regarding the selectivity and those of a classic Solid Phase Extraction (SPE) in terms of costs and robustness. The advantages of AFFINIMIP SPE are essential in trace analysis from a complex matrice in food safety, environment and pharmaceutical analysis such as Mycotoxins (Patulin, Ochratoxin A, Zearalenone), phenolics, endocrines disruptors (estrogens), Bisphenol A, GSH adducts, etc. AFFINIMIP SPE Selective Extraction Cartridges Perfect clean-up system for trace analysis Thanks to selectivity of AFFINIMIP SPE, stringent washing steps can be applied in order to remove all interferences and thus minimize matrix effects. It also reduces ion-suppression effects. The SPE protocol is supplied in an instruction sheet for various complex matrices. For other matrices, please contact our technical support to help you with your application. Minimal to no method development required A protocol based on three steps (loading, washing and elution) is supplied with AFFINIMIP SPE kits for tested matrices. No extra-equipment than the usual required for SPE experiments is necessary. AFFINIMIP SPE protocols are as well defined by 3 steps of loading, washing and elution. All steps have been already developed in detail by POLYINTELL and an instruction sheet is supplied with the product. Example of an instruction sheet supplied with AFFINIMIP SPE 11

12 AFFINIMIP SPE VS IMMUNOAFFINITY COMPARATIVE STUDY Solid phase extraction is a simple tool to selectively extract analytes from complex matrices and quantify concentrations lower and lower. The major disadvantage of conventional SPE sorbents, such as C18 is a lack of selectivity and interference matrix components are co-extracted with the target analytes. To solve this problem, affinity-based SPE sorbents have been developed to be selective in extracting the target analytes like molecularly imprinted polymer (MIP) and immunoaffinity sorbent. Immunoaffinity columns (IAC) are biological sorbents based on the use of antibodies that are specific to the target analytes. Molecularly imprinted polymer is a synthetic material with artificially generated three-dimensional network able to specifically rebind a target molecule. Based on molecularly imprinted polymers, POLYINTELL s AFFINIMIP SPE cartridges have the advantages to be highly selective and specific. Contrary to IAC, AFFINIMIP SPE cartridges are chemically and thermally stable, compatible with all solvents as well as cost effective. PROPERTIES OF MIP AND IAC Feature IAC AFFINIMIP SPE Selectivity High High Capacity Analyte recognition in water Analyte recognition in Organics 6µmol/g Good Poor µmol/g Variable Good Stability Poor Very High Reproducibility Variable Good Cost Expensive Inexpensive PROTOCOL: Zearalenone (ZON) from maize flour Step Extraction of target analyte Preparation loading solution Loading Washing Elution Protocol time Vicam IAC 25g sample in 100mL 90/10 Methanol/water Blender 3 minutes + filtration 4mL extract + 96mL water 100mL Loading solution 20mL Water 1.5mL Methanol 55min AFFINIMIP SPE ZON 25g sample in 100mL 75/25 ACN/water Blender 3 minutes + filtration 10mL extract + 10mL Water 8mL Loading solution 4mL 2/58/40 Acetic acid / water / ACN 2mL 98/2 Methanol/Acetic acid 30min Compared to IAC, AFFINIMIP SPE provides: Easier and faster protocol Lower dilution Easier automatisation (Cf. Automated method for the selective SPE of Ochratoxin A from wheat Using Molecularly Imprinted Polymer; Gilson Application Notes Handbook 2011; volume 1 Issue 4) PROTOCOL: Ochratoxin A (OTA) from wheat flour Step Extraction of target analyte Preparation loading solution Loading Washing Elution Vicam IAC AFFINIMIP SPE OTA 50g sample in 100mL 60/40 ACN/water Blender 1 minute + filtration 10mL extract + 40mL PBS 10mL Loading solution 10mL PBS 10mL Water 1.5mL Methanol 10mL extract + 10mL HCl 0.1M ph=1 4mL Loading solution 7mL 60/40 HCl 0.1M ph=1/acn 2mL 98/2 Methanol/Acetic acid Protocol time 30min 20min

13 mvolts mvolts Area Fluo elution B06 Fluo elution immuno B Minutes mvolts mvolts Level found ng/g mvolts mvolts AFFINIMIP SPE CARTRIDGE VS IMMUNOAFFINITY COLUMN CHROMATOGRAM ASPECT Equivalent chromatograms IAC AFFINIMIP SPE Minutes Minutes Figure 1. Chromatogram of Maize sample spiked with Zearalenone at 85 µg/kg obtained after cleanup by AFFINIMIP SPE Zearalenone (red) or Vicam IAC (blue). RECOVERIES Higher Recoveries obtained with AFFINIMIP SPE 10ng/g OTA 85% Recovery Recovery % ZON 85 OTA AFFINIMIP SPE IAC 6ng/g OTA 79% Recovery Figure 2. Chromatogram of wheat sample spiked with Ochratoxin A obtained after cleanup by AFFINIMIP SPE Zearalenone (red, spiked at 10ng/g) or Vicam IAC (blue, spiked at 6ng/g). 0 MIP IAC MIP IAC Figure 3. Recovery of Ochratoxin A or Zearalenone obtained after cleanup by AFFINIMIP SPE or Vicam IAC. CAPACITY Capacity MIP > Capacity IAC 3,50E+08 3,00E+08 2,50E+08 2,00E+08 1,50E+08 1,00E+08 5,00E+07 AFFINIMIP SPE IAC 0,00E Spiking level ng/g Figure 4. Comparison of capacity between AFFINIMIP SPE Zearalenone (red) and Vicam IAC (blue) AFFINIMIP SPE IAC Spiking level ng/g Figure 5. Comparison of capacity between AFFINIMIP SPE OTA (red) and Vicam IAC (blue).

14 AFFINIMIP SPE available APPLICATIONS Mycotoxins AFFINIMIP SPE FIELD MATRICES REF Zearalenone Food Safety, Veterinary field Maize, Wheat, Cereal-based baby food, Rice, Edible corn oil Ochratoxin A Food Safety Wheat, Maize, Pepper, Paprika, Red and White Wine Patulin Food Safety Apple juice, Apple and Multifruit Purees, Baby Food, dried apple, cider, Alcohol pommeau and manzella FS100 FS101 FS102 Fumonisins AND Zearalenone Food Safety Maize, Maize-based baby food FS109 Tamoxifen Pharma R&D, doping control Urine PH101 Bisphenol A Environment, Food, Water, Milk (infant formula), Powdered infant formula, Canned food FS106 Chloramphenicol Food Safety, veterinary field Honey, Bovine Urine FS110 Glutathione (GSH) Toxicology, Pharma R&D Microsomes PH100 Prep GSH adducts Toxicology, Pharma R&D Microsomes PH103 Estrogens Ex: Estrone, 17α-Estradiol, 17β-Estradiol, Estriol, 17α- Ethynilestradiol Environment, Veterinary field Water, Serum, Plasma FS104 Zeranol Residues Ex: Zeranol, Zearalanone α and β Zearalanol, α and β Zearalenol, Resorcylic acid lactones Veterinary field, Food and Feed Safety Urine, Plasma FS105 Catecholamines Ex: Dopamine, Noradrenaline, Adrenaline,... Metanephrines Ex: Metanephrine, Normetanephrine and 3- Methoxytyramine,... Life science, pharma R&D Plasma, Serum DG100 Life science, pharma R&D Plasma, Serum DG101 Amphetamines Ex: Amphetamine, Methamphetamine and their derivatives (MDA, MDMA, MDEA ) Serum, Urine DG102 Phenolics Ex: Parabens, Hydroquinone, Drugs Pharma, Cosmetic, Environment, Food Safety,. Water, Meat, Cereal, Serum, Urine, FS103 14

15 Area mv AFFINIMIP SPE Zearalenone FS100 AFFINIMIP SPE Zearalenone are selective solid-phase extraction cartridges for Zearalenone from complex matrices such as Maize, Cereal-based Baby Food and Rice. In Europe, Regulation (EC) N 1881/2006 sets maximum levels for Zearalenone in foodstuffs such as 100µg/kg in cereals and 20µg/kg in maize-based babyfood. Perfect clean-up method for trace analysis of raw and cooked food matrices CEREAL SAMPLE With AFFINIMIP SPE Zearalenone No pre-treatment Zearalenone Time (min) Fluorescence chromatograms obtained after a purification with AFFINIMIP SPE Zearalenone of a cereal sample (80µg/kg of Zearalenone) Very simple protocol for complex matrices Tested matrices : Wheat, Maize, Cereal-based baby food, Edible corn oil and Rice Capacity: Higher than 10µg/g Zearalenone Protocol : Loading : Acetonitrile - Water Washing : Acetonitrile - Water - Acetic Acid Elution : Methanol - Acetic acid Detection : HPLC-Fluorescence (excitation/emission wavelengths of 275 / 450nm) Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support High capacity - No need of high dilution High capacity of AFFINIMIP SPE in comparison with Immuno Affinity Columns Comparative study of the capacity between Immuno- Affinity Columns and AFFINIMIP SPE Zearalenone Publications Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts, Analytica Chimica Acta, 672, 15 19, Immuno-Affinity Columns AFFINIMIP SPE Zearalenone Application note - AFFINIMIP SPE Zearalenone - Cereal Products and Baby Food Application note - AFFINIMIP SPE Zearalenone - Automated Protocol (GILSON GX271 ASPEC ) Application note - AFFINIMIP SPE Zearalenone Edible corn oil Zearalenone (ng) Ordering information: Catalog number: FS for 25 cartridges, 3mL FS for 50 cartridges, 3mL 15

16 mv AFFINIMIP SPE Ochratoxin A FS101 AFFINIMIP SPE Ochratoxin A are selective solid-phase extraction cartridges for Ochratoxin A that selectively clean and concentrate the toxin prior to analysis by HPLC from matrices such as Wheat, Maize, Pepper, Paprika, Red and White Wine. In Europe, Regulation (EC) N 1881/2006 sets maximum levels for Ochratoxin A in foodstuffs such as 5µg/kg in cereals and 2ppb in wine. The regulation (EC) N 105/2010 also defines maximum levels for spices and liquorice products Ready for use and optimized extraction protocols adapted for each tested matrices Perfect clean-up system for trace analysis of Ochratoxin A Unambiguous and reliable analysis Matrix Recovery yield C Ochratoxin A (µg/kg) Recoveries % % RSD Wheat (n=6) Paprika (n=4) Grey Pepper (n=3) White wine (n=10) WHEAT With AFFINIMIP SPE Ochratoxin A No pre-treatment Ochratoxin A Time (min) Fluorescence chromatograms obtained after a purification with AFFINIMIP SPE Ochratoxin A of a wheat sample (2.5µg/kg of Ochratoxin A) Red wine (n=4) Tested matrices : Wheat, Maize, Paprika, red and white Wine, Pepper (format SPICES: 6mL) Protocol : Loading : loading solution Washing : Acetonitrile - HCl solution Elution : Methanol - Acetic acid Detection : HPLC-Fluorescence excitation/emission wavelengths of 333 and 460nm Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Publications Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals, Journal of Chromatography A, 1217, , 2010 Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine, Tien Ping Lee et al., Talanta, 88, , 2012., Accepted manuscript Application note - AFFINIMIP SPE Ochratoxin A - Cereals and Spices Application note - AFFINIMIP SPE Ochratoxin A - Red and White Wine Application note - AFFINIMIP SPE Ochratoxin A - Automated Protocol (GILSON GX271 ASPEC ) Ordering information: Catalog number: FS for 25 cartridges, 3mL FS for 50 cartridges, 3mL Format Ochratoxin A SPICES for Pepper FS101PP-02 for 25 cartridges, 6mL FS101PP-03 for 50 cartridges, 6mL 16

17 mau AFFINIMIP SPE Patulin FS102 AFFINIMIP SPE Patulin are selective solid-phase extraction cartridges that selectively clean and concentrate this toxin prior to analysis by HPLC from complex matrices such as apple juice, compote, apple puree including based on baby food. The World Health Organization, the FDA and the European Union recommend a maximum concentration of 50 µg/l in apple juice and 25µg/kg in apple puree. Unique extraction method available on the market Easier and faster than Liquid Liquid Extraction Perfect clean-up system for trace analysis of Patulin Ready for use and optimized extraction protocol Applicable to several apple derived matrices Considerable decrease of 5-Hydroxymethylfurfural (HMF) signal Matrix C Patulin (ng/ml) Recoveries % % RSD Apple juice (n=4) Apple juice (n=4) Apple juice (n=4) Apple puree (n=3) Baby food apple puree (n=4) APPLE JUICE Patulin Time (min) LC-UV chromatograms obtained after AFFINIMIP SPE Patulin Clean-up of an apple juice spiked at 10µk/kg with Patulin (blue) and not spiked (Red) Recovery yield Tested matrices: Apple juice (clear & cloudy), Apple and Multifruit puree, baby food, cider, Alcohol pommeau and manzella, Dried apple (format 200mg) Protocol: Loading: Diluted apple juice or apple puree after treatment with pectinase enzyme. Washing: NaHCO3 Water - Diethyl Ether Elution: Ethyl acetate Detection: HPLC-UV Format: 3 or 6mL cartridges depending on matrix Particle diameter range : µm Stability: 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Publications Application note - AFFINIMIP SPE Patulin - Apple Juice and Apple Puree Application note - AFFINIMIP SPE Patulin - Baby Food Application note - AFFINIMIP SPE Patulin - Cider Application note - AFFINIMIP SPE Patulin - Multifruit Puree Application note - AFFINIMIP SPE Patulin Dried apple Ordering information: Catalog number: FS for 25 cartridges, 3mL-100mg sorbent FS for 50 cartridges, 3mL-100mg sorbent FS102-02K for a kit of 25 cartridges (3mL) + 50mL Pectinase FS102-03K for a kit of 50 cartridges (3mL) + 50mL Pectinase For a DRIED APPLE or higher enrichment with apple juice FS mg for 25 cartridges, 6mL-200mg sorbent FS mg for 50 cartridges, 6mL-200mg sorbent 17

18 Intensity Intensity Intensity AFFINIMIP SPE FumoZON FS109 AFFINIMIP SPE FumoZON are selective solid-phase extraction cartridges that selectively and SIMULTANEOUSLY clean and concentrate Fumonisins and Zearalenone prior to analysis by HPLC from complex matrices such as maize and cereal-based baby food. In Europe, Regulation (EC) N 1126/2007 sets maximum levels for Zearalenone and Fumonisins in foodstuffs with respectively 20µg/kg and 200µg/kg for maize-based babyfood. RT: RT: SIMULTANEOUS Selective Extraction of Fumonisins B1+B2 and Zearalenone (ZON) for analysis by LCMS Ready for use and optimized extraction methodology adapted to maize matrices Strong reduction of Ion suppression Very simple protocol and high capacity cartridges Time (min) 6.19 MAIZE Fumonisin B2 Fumonisin B1 Zearalenone Time (min) Chromatograms obtained after AFFINIMIP SPE FumoZON Clean-up of a maize flour spiked at 38µg/kg with Zearalenone, 2408µg/kg with Fumonisin B1 and 630µg/kg with Fumonisin B2. Tested matrices : maize matrices Capacity: Higher than 10µg/g Zearalenone and 5µg/g Fumonisins B1 and B2 Protocol : Loading : Methanol Acetonitrile - Water Washing : Acetonitrile - Water Elution : Methanol - Acetic acid Detection : HPLC-MS ESI Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Recovery yield Recovery of Zearalenone, Fumonisins B1 and B2 in maizebased baby food after AFFINIMIP SPE FumoZON cleanup Sample C µg/kg Mean µg/kg Recoveries % % RSD R Zearalenone (n=4) Fumonisin B (n=3) Fumonisin B (n=3) Publications Application note - AFFINIMIP SPE FumoZON - Maize and Maize-based baby food Ordering information: Catalog number: FS for 25 cartridges, 3mL FS for 50 cartridges, 3mL 18

19 signal (mv) AFFINIMIP SPE Tamoxifen PH101 Since January 2000, Tamoxifen (antioestrogenic molecule) has been included in the list of prohibited substances by the International Olympic Committee. So, the presence of these compounds in urine is a doping proof. The analysis of complex samples, like biofluids, requires a sample preparation step prior to analysis. AFFINIMIP SPE Tamoxifen is the perfect tool for selectively extract Tamoxifen and its main metabolite in biofluids, such as urine (clean-up and preconcentration of sample at trace levels). The recoveries of Tamoxifen and its metabolite 4-HydroxyTamoxifen (4-OH Tam) are greater than 80% at 10 ng/ml. Perfect clean-up system for trace analysis of Tamoxifen and its metabolite in urine Unambiguous and reliable analysis Comparison between HLB and AFFINIMIP SPE Tamoxifen URINE HLB AFFINIMIP SPE 4-OH Tamoxifen Tamoxifen Tested matrices : Urine Protocol : Loading : Urine Washing : Water- Methanol Elution : Methanol - Acetic acid Detection : HPLC-UV Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Time (min) LC-UV comparison of urine sample spiked with tamoxifen and 4-OH tamoxifen (its main metabolite) after extraction through HLB and AFFINIMIP SPE Publications Interest of molecularly imprinted polymers in the fight against doping. Extraction of tamoxifen and its main metabolite from urine followed by high-performance liquid chromatography with UV detection. Journal of Chromatography A, , 81 88, Ordering information: Catalog number: PH for 25 cartridges, 3mL PH for 50 cartridges, 3mL 19

20 AFFINIMIP SPE Bisphenol A FS106 AFFINIMIP SPE Bisphenol A are selective solid-phase extraction cartridges that selectively clean and concentrate Bisphenol A prior to analysis by HPLC from complex matrices such as River Water or Babymilk. It is known as an endocrine disruptor and has been banned in plastic infant feeding bottles in several countries (Canada, EU (directive 2011/8/EU)). Perfect clean-up system suitable for all chromatography techniques: LC, GC, MS... Easy quantification of Bisphenol A with fluorescence detection : no derivatization as needed in GC 2 grades: glass and polypropylene cartridges LIQUID INFANT FORMULA Injection of 50µL of infant formula before clean-up Bisphenol A after clean-up of 15mL of infant formula Minutes Time (min) Fluorescence chromatograms of infantil formula spiked with 1µg/L Bisphenol A before (Red) and after purification (Blue) with AFFINIMIP SPE Bisphenol A. Tested matrices : Water, milk (infant formula), powdered infant formula or canned food Protocol: Loading: Matrix Washing: Water - Acetonitrile Elution: Methanol Format: Polypropylene cartridges 3mL Glass cartridges 3mL with Teflon frits: max. quantity BPA < 5ng/cartridge Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Publications Application note - AFFINIMIP SPE Bisphenol A - Beer Application note - AFFINIMIP SPE Bisphenol A - infant formula Application note - AFFINIMIP SPE Bisphenol A - Powdered infant formula Application note - AFFINIMIP SPE Bisphenol A Canned food Application note - AFFINIMIP SPE Bisphenol A Vegetable puree in plastic food packaging Ordering information: Catalog number: FS for 25 cartridges, 3mL PP cartridges FS for 50 cartridges, 3mL PP cartridges FS106-02G for 25 cartridges, 3mL glass cartridges FS106-03G for 50 cartridges, 3mL glass cartridges 20

21 mvolts 2-MD1.dat Fluo 2-md2.dat Fluo 4-md1.dat Fluo 4-md2.dat Fluo mnd.dat Minutes Protocol Comparison AFFINIMIP SPE Bisphenol A vs competitor FS106 Performance. Save your time. POWDERED INFANT FORMULA ANALYSIS POLYINTELL method Precipitation (15g sample + 15mL ACN) Centrifuge and collect supernatant Alternative method Precipitation (10g sample + 10mL ACN) Centrifuge and collect supernatant Dilute 20mL of supernatant with 20mL H 2 0 Load on AFFINIMIP SPE Bisphenol A 3cc (40mL) Wash 10mL H 2 0 and 6mL 40% ACN ; Elute 3mL 100% MeOH HPLC analysis : LC Fluorescence detector or GC-MS/MS or LC-MS/MS POWDERED INFANT FORMULA Quecher 1 Add contents from Quecher tube 1. Shake. Centrifuge and collect 10mL Quecher 2 Add contents from Quecher tube 2. Shake. Centrifuge and collect supernatant Dilute supernatant with 70mL H20 Load on Polymeric SPE cartridge 3cc (70mL) Wash 2mL 40% Methanol ; Elute 1mL 100% MeOH 1400 Fluo Bisphenol A HPLC analysis : LC-MS/MS ,5 7,0 7,5 8,0 8,5 9,0 9,5 10,0 10,5 11,0 11,5 12,0 12,5 13,0 13,5 14,0 Chromatograms obtained after clean-up with AFFINIMIP SPE Bisphenol A of equivalent at 10mL of Infant Formula spiked with Bisphenol A at 4.3µg/L (tested twice, red) or at 2.1µg/L (tested twice, blue) or not spiked (pink). Ordering information: Catalog number: FS for 25 cartridges, 3mL PP cartridges FS for 50 cartridges, 3mL PP cartridges FS106-02G for 25 cartridges, 3mL glass cartridges FS106-03G for 50 cartridges, 3mL glass cartridges 21

22 intensity AFFINIMIP SPE Chloramphenicol FS110 AFFINIMIP SPE Chloramphenicol are selective solid phase extraction cartridges that selectively clean and concentrate Chloramphenicol from complex matrices such as Honey. Chloramphenicol is a broad-spectrum antibiotic widely used in the world in the past. Several health problems are related by its use. As a consequence, several countries (e.g. U.S.A, E.U, Canada ) have prohibited its use for food-producing animals. As no permited limit has been established, E. U has defined a minimum required performance limits (MRPLs) of 0.3µg/kg for products of animal origin (Commission decision 2003/181/EC). However, food matrices are very complexes and induce ion-suppression phenomena which distort analysis results. AFFINIMIP SPE Chloramphenicol solves this problem. Very simple and fast sample preparation Direct percolation of honey diluted in water High loading capacity: Up to 10g of honey analysed Perfect clean-up system for trace analysis of Chloramphenicol Detection of 0.3µg/kg with a single quatrupole Mass spectrometer HONEY Recovery yield > 90% Tested matrices : Honey, Bovine Urine Protocol: Loading: Diluted honey Washing: ACN Water NH3 Diethyl ether Elution: Methanol Format: Polypropylene cartridges 1mL Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Time (min) MS chromatogram (SIM) of several honeys spiked with 15.7 µg/kg of Chloramphenicol after clean-up with AFFINIMIP SPE Chloramphenicol Blue traces are not spiked samples Publications Application note - AFFINIMIP SPE Chloramphenicol Honey Application note - AFFINIMIP SPE Chloramphenicol Bovine Urine Ordering information: Catalog number: FS110-02A for 25 cartridges, 1mL FS110-03A for 50 cartridges, 1mL 22

23 Recoveries % AFFINIMIP SPE GSH PH100 In drug discovery, the potential of reactive intermediate formation is currently assessed with a reactive endogenous nucleophile such as GSH. AFFINIMIP SPE GSH are selective solid-phase extraction cartridges for the extraction of GSH adducts from incubations with hepatic microsomes or hepatocytes containing an excess of GSH and drug candidates. Separation of GSH, GSH adducts and a drug candidate with AFFINIMIP SPE GSH A reliable solution for GSH adduct detection Very SIMPLE and UNIQUE protocol: Short development time for each adduct NOW AFFINIMIP SPE GSH 96-WELL PLATE AVAILABLE AFFINIMIP SPE GSH allows the separation between GSH, GSH adduct(s) and the drug candidate. The drug candidate is eliminated during the loading and the washing step while GSH adduct(s) are only extracted in the elution step. This collection fraction can be subject to classical analytical methods to detect GSH adducts with a better sensitivity. AFFINIMIP SPE GSH permits a specific retention of free GSH which is completely removed L 0 MICROSOMES 43 0 W 0 55 Recovery yields in each fraction of AFFINIMIP SPE GSH for free GSH, drug (Acetaminophen) and GSH adducts 0 E GSH adducts Acetaminophen Free GSH Product information Tested matrices : Microsomes incubation Protocol : Loading : Incubation solution - Acetic Acid - Acetonitrile Washing : Acetonitrile - Water - Acetic Acid Elution : Acetonitrile - Water Detection : LC-MS Format : 3mL cartridges and 96-Well plate Particle diameter range : µm Stability : 1 year Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Publications Application note - AFFINIMIP SPE GSH Ordering information: Catalog number: PH for 25 cartridges, 3mL PH for 50 cartridges, 3mL PH W for 1 96-well plate 23

24 Intensity AFFINIMIP SPE PREP GSH ADDUCTS PH103 AFFINIMIP SPE PREP GSH ADDUCTS is a selective extraction cartridge for an easy semi-preparative isolation and purification of GSH conjugates for structural characterization by NMR. Ready for use and optimized extraction methodologies Complete removal of free GSH and tested molecules Fast, simple & specific sample preparation method Principle : This product is composed of two resins : The first resin reacts as a scavenger of GSH by reacting covalently with all free GSH (its capacity to react with GSH is about 30 to 40 µmol/g ) The second resin is an imprinted polymer able to non covalently entrap all GSH conjugates (its capacity to trap is about 4 to 6 µmol/g ) and separate them from other molecules. Application : The incubation mixture containing an excess of GSH, drug candidate and traces of GSH adduct(s) is loaded on AFFINIMIP SPE PREP GSH Adducts Loading (L) Washing (W) Elution (E) Removal of other molecules Purified GSH-ADDUCTS Results : Total-ion current profile obtained after an extraction on AFFINIMIP SPE PREP GSH Adducts 10g. Purification of an incubation of 10mL containing 5mM of GSH No GSH and drug candidate observed GSH adduct Tested matrices : Microsomes incubation Protocol : Loading : Incubation solution - Acetic Acid - Acetonitrile Washing : Acetonitrile - Water/Acetic Acid Elution : Acetonitrile - Water Detection : LC-MS Format: cartridges of 10g or 30g Particle diameter range : µm Stability : 1 year Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Ordering information: Catalog number: PH for 2 cartridges, 10g resin for 10-15mL incubation (5mM GSH) PH for 2 cartridges, 30g resin for 15-50mL incubation (5mM GSH) 24

25 AFFINIMIP SPE Estrogens FS104 AFFINIMIP SPE Estrogens are selective solid-phase extraction cartridges that selectively clean and concentrate the natural or synthetic estrogens family prior to further analysis from complex matrices such as Water, Plasma or Serum. Extraction of a broad family of natural and synthetic estrogens by direct percolation of diluted plasma or water Fast and simple protocol BOVINE PLASMA Blank plasma 10ppt 40ppt 100ppt 17β-Estradiol-d 3 419> α/β-estradiol 416> α/β-estradiol 416>285 MRM chromatograms from GC-MS/MS analysis of fortified calves plasma samples at 0, 10, 40 and 100 pg.ml -1 with 17αestradiol, 17β-estradiol and estrone. Chromatograms obtained after a clean-up with AFFINIMIP SPE Estrogens (Courtesy of Emmanuelle Bichon - LABERCA) Tested matrices : Water and plasma. Protocol : Loading : Water or diluted plasma Washing : Acetonitrile - Water Elution : Methanol Detection : LC-MS Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Publications Solid-phase extraction using molecularly imprinted polymer for selective extraction of natural and synthetic estrogens from aqueous samples, Paolo Lucci, Oscar Núñez, M.T. Galceran, Journal of Chromatography A, 1218(30), , 2011 Quantification of estrogens at ppt levels in bovine plasma by Molecularly Imprinted Solid Phase Extraction and GC- MS/MS analysis, Emmanuelle Bichon et al. (LABERCA) Poster session, HTSP-2 and HTC 2012 Ordering information: Catalog number: FS for 25 cartridges, 3mL FS for 50 cartridges, 3mL 25

26 AFFINIMIP SPE Zeranol Residues FS105 AFFINIMIP SPE Zeranol Residues are selective solid-phase extraction cartridges for Zeranol and its residues from complex matrices such as urine or meat. Zeranol (or α-zearalanol) is a potent nonsteroidal growth promoter used extensively in the United States and Canada to promote rapid growth rates in animals. Nevertheless, its use is banned in several countries (e.g. European directive 96/22/EC and in China). These cartridges can extract Zeranol, Zearalenone, β-zearalanol (Taleranol), α and β-zearalenol, Zearalanone and more broadly, Resorcylic acid lactones. The analysis of these compounds in urine or meat requires a long and tedious pre-treatment sample (several Liquid-Liquid Extractions and multi SPE steps). AFFINIMIP SPE Zeranol Residues provides similar results in an easier manner. These analyses are sensitive, rapid and reproducible. Extraction of a broad family of Zeranol residues and Resorcylic acid lactones compounds Ready for use and optimized extraction protocols adapted for each tested matrices Very simple and short pre-treatment sample CATTLE URINE SPIKED AT 1ng/mL of 6 main Zeranol metabolites Taleranol-d4/ Zeranol-d4 Taleranol/ Zeranol α and β- Zearalenol Zearalenone Zearelenone Quantification of a mixture (Zeranol, Zearalenone, β- Zearalanol, α and β-zearalenol and Zearelenone) in bovine urine spiked at 1ng/mL. Comparison of a clean-up made with AFFINIMIP SPE Zeranol Residues + NH 2 cartridges and with IAC clean-up. (Courtesy of Emmanuelle Bichon - LABERCA) Tested matrices : Urine and plasma. Protocol : Loading : loading solution Washing : Acetonitrile - Water - Acetic acid Elution : Methanol - Acetic acid Detection : HPLC-Fluorescence or LC-MS Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Publications New technological tools for isolating and measuring growth promoting agents in edible tissues and biological fluids, Emmanuelle Bichon et al. (LABERCA) Poster session, RAFA 2011 Ordering information: Catalog number: FS for 25 cartridges, 3mL FS for 50 cartridges, 3mL 26

27 AFFINIMIP SPE Catecholamines DG100 AFFINIMIP SPE Catecholamines are selective solid-phase extraction cartridges that selectively clean and concentrate the Catecholamines and its amino metabolites from complex matrices such as Plasma or Serum. A reliable solution for extraction of Catecholamines and its metabolites Good clean-up method Free catecholamines Dopamine (DA) Norepinephrine or Noradrenaline (NA) Epinephrine or Adrenaline (A) Metabolites Metanephrine Normetanephrine (NMN) 3-Methoxytyramine NA SERUM A DA NMN HMBA I.S. Tested matrices : Plasma and serum Protocol : Loading : diluted serum or plasma Washing : Water - Methanol Elution : Methanol - Acetic acid Detection : HPLC-UV Format: 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support LC-UV chromatogram of a serum sample spiked with Catecholamines at 450nM with ( ) and without ( ) AFFINIMIP SPE Catecholamines Internal Standard: 4-hydroxy-3-methoxybenzylamine (HMBA) (Courtesy of B. Claude and P. Morin, ICOA, Orléans, France) Recovery yield Analytes Recoveries % Dopamine (DA) 67 Noradrenaline (NA) 80 Adrenaline (A) 84 3-Methoxytyramine 102 Normetanephrine (NMN) 106 Publications Analysis of urinary neurotransmitters by capillary electrophoresis: Sensitivity enhancement using fieldamplified sample injection and molecular imprinted polymer solid phase extraction, Bérengère Claude et al., Analytica Chimica Acta, 699 (2), , Ordering information: Catalog number: DG for 25 cartridges, 3mL DG for 50 cartridges, 3mL DG100-02A for 25 cartridges, 1mL DG100-03A for 50 cartridges, 1mL 27

28 Intensity AFFINIMIP SPE Metanephrines DG101 AFFINIMIP SPE Metanephrines are selective solid phase extraction cartridges that selectively clean and concentrate Metanephrines from complex matrices such as Plasma or Serum. Quantification of free metanephrines in Plasma (Metanephrine, Normetanephrine and 3-methoxytyramine) is considered to be a highly sensitive test for the diagnosis of Pheochromocytoma and the follow-up of patients. Free Metanephrines are in a very low concentration in this complex matrix which makes a reliable determination of the compound an analytical challenge. Extraction of Metanephrines by direct percolation of diluted plasma or serum MeO HO OH N H Me MeO HO OH NH 2 MeO HO NH 2 Metanephrine (MN) Normetanephrine (NMN) 3-methoxytyramine (3MT) NMN MN SERUM 3MT Tested matrices : Plasma and serum Protocol : Loading : diluted serum or plasma Washing : Water - Methanol Elution : Methanol - Acetic acid Detection : HPLC-UV Format: 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Time (min) Mass chromatogram (SIM+) of plasma spiked with 60nM of Metanephrines after clean-up with AFFINIMIP SPE Metanephrines MN: Metanephrine (m/z=180); NMN: Normetanephrine (m/z=166); 3MT: 3-Methoxytyramine (m/z=151) (Courtesy of B. Claude and P. Morin, ICOA, Orléans, France) Publications Application note - AFFINIMIP SPE Metanephrine in plasma - comparison with WCX Ordering information: Catalog number: DG for 25 cartridges, 3mL DG for 50 cartridges, 3mL DG101-02A for 25 cartridges, 1mL DG101-03A for 50 cartridges, 1mL 28

29 AFFINIMIP SPE Amphetamines DG102 AFFINIMIP SPE Amphetamines are selective solid phase extraction cartridges that selectively clean and concentrate Amphetamine and Metamphetamine derivatives from complex matrices such as Serum or Urine. Amphetamine has a high possibility of severe psychological and physiological dependence. As a stupefiant, Amphetamines consumption is prohibited in main european countries. French regulation has set up an analytical threshold of 50ng/mL in blood for drivers. Extraction of Amphetamine and Methamphetamine derivatives by direct percolation of diluted serum or urine High capacity cartridge for reliable results at high concentrations Recoveries >85% for each analyte Reduction of sample preparation time Amphetamine Methamphetamine MDA MDMA MDEA SERUM Time (min) Mass chromatogram (SIM) of 0.5mL of serum spiked with 100ng/mL of each Amphetamine and Methamphetamine derivatives after clean-up with AFFINIMIP SPE Amphetamines Amphetamine (m/z=136); Methamphetamine (m/z=150); MDA: 3,4-Methylenedioxyamphetamine (m/z=180); MDMA, 3,4-Methylenedioxymethamphetamine (m/z=194); MDEA, 3,4-Methylenedioxy-N- ethyl amphetamine (m/z=208). Recovery yield Tested matrices : Urine and serum Protocol : Conditioning : Acetonitrile and Deionized Water Loading : Diluted urine or serum Washing : W1: Deionized Water W2: Acetonitrile - Deionized Water Elution : Methanol - Formic acid Detection : HPLC-MS ESI positive mode Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Recovery yield and %RSD for serum sample spiked with 100 ng/ml (n=4) Analyte Recoveries % % RSD Amphetamine Methamphetamine MDA MDMA MDEA Publications Application note - AFFINIMIP SPE Amphetamines - Human urine and serum Ordering information: Catalog number: DG for 25 cartridges, 3mL DG for 50 cartridges, 3mL 29

30 mv AFFINIMIP SPE Phenolics CLASS SPECIFIC FS103 AFFINIMIP SPE Phenolics are selective solid-phase extraction cartridges that selectively clean and concentrate a broad range of phenolic compounds prior to further analysis. Phenolic compounds are an important family of products found as natural substances in plants and life sciences or as synthetic products such as drugs. Phenolics include a large group of several hundred chemical compounds, characterized by having at least one aromatic ring with one hydroxyl group attached. Extraction of a broad range of Phenolic compounds Reduction of matrix effects and of most interferents Several protocols proposed to purify your analyte Phenolic compound Example of extraction of Phenolic compounds from complex matrix Before MIP clean-up Elution after loading of 10mL of diluted complex matrix spiked with: 150ng/mL of analyte 100ng/mL of analyte Time (min) HPLC profile with a fluorescence detection Protocol : Protocol with an organic loading solution Loading : Acetonitrile Washing : Acetonitrile - Methanol Elution : Methanol - Acetic Acid Protocol with an hydro-organic loading solution Loading : Acetonitrile-Water Washing : Acetonitrile - Water Elution : Methanol Format : 3mL cartridges Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature For other matrices or formats, please contact us for technical support Example of phenolic molecules which can be purified Parabens Tocopherols Nitrophenols Chlorophenols Catechins Quercetins Etc Ordering information: Catalog number: FS for 25 cartridges, 3mL FS for 50 cartridges, 3mL 30

31 AFFINIMIP SPE 18 F Selective Solid Phase Extraction For Radiopharmaceutical purification Simplify your radiotracer Purification with AFFINIMIP SPE 18 F Positron Emission Tomography (PET) is a nuclear imaging technique having an ever increasing importance in diagnostic medicine nowadays. PET commonly utilizes the radioactive forms of carbon ( 11 C), nitrogen ( 13 N), oxygen ( 15 O) and fluorine ( 18 F). The use of these isotopes allows the labelling of many different substrates without altering their biological activity. The half lives of some nuclei are relatively short, which poses a timescale problem for radiochemists and can leave little room for manoeuvre between introducing the radioisotope into the tracer, and conducting the PET scan. Of these isotopes 18 F has the most convenient half life of minutes. Purification is the critical step in radiotracer production Due to the short lifetime of radiotracers, the method of purification is a key aspect in radiopharmaceutical production and long purification steps must be avoided. The method of choice must also be effective enough to ensure a high level of radiochemical purity. How to solve this? POLYINTELL has developed a fast and powerful method to purify 18 F radiotracers using the affinity of its Molecularly Imprinted Polymers. For purification of radiotracers, AFFINIMIP SPE is based on the affinity for the precursor which is retained on the cartridges. In the case of Aromatic Nucleophilic Substitution with 18 F, impurities, Kryptofix and 18 F are also retained on the cartridge. Then the purified radiotracer is obtained after elution. 100 % of radiotracer Purification of radiotracers using AFFINIMIP SPE 18 F Rapid and simple radiotracer purification: no need for a preparative HPLC thanks to our All-in-One SPE Increased reliability of radiotracer production: reduction of manual handling steps Improved radiotracer purity: no cross contamination thanks to single use cartridges compatible with radiofluoration automates 31

32 AFFINIMIP SPE 18 F - Aromatic Nucleophilic Substitution AFFINIMIP SPE 18 F - Aromatic Nucleophilic Substitution is a selective solid phase extraction cartridge used to purify rapidly and perfectly a 18F radiotracer synthesized from an aromatic nucleophilic substitution. The cartridge retains the ammonium-based precursor as well as the phenol- and dialkylamino-based impurities. N + Precursor F -, Kryptofix ACN or DMSO F Pure Radiotracer Rapid and simple method: No need for a preparative HPLC thanks to our All-in-One SPE Improved radiotracer purity: No traces of the 3 unwanted products (precursor and by products) and low traces of Kryptofix and of additional 18 F were detected. Increased reliability of radiotracer production: Reduction of manual handling steps No cross contamination thanks to single use cartridges The fluorination was carried out in the presence of Kryptofix and potassium fluoride in Acetonitrile at 95 C. The crude mixture was loaded on the SPE cartridge, washed and the fluorous radiotracer was eluted in Acetonitrile or in the minium of Ethanol. Example of Application : 4-Fluoro Ethylbenzoate HO Precursor By-products radiotracer N + O O Kryptofix ACN or DMSO F Precursor Radiotracer By-products The HPLC analysis shows that the elution fraction was constituted of the radiotracer (more than 95 % recoveries) and no trace of the ammonium-based precursor and the known impurities were detected. Low traces of Kryptofix and 18 F were detected. HLB cartridges can be associated to switch your radiotracer in Acetonitrile to the minimum of Ethanol. Publications Application note - AFFINIMIP SPE 18 F Aromatic Nucleophilic Substitution O O + N O O O O UV Chromatograms (235nm) obtained before (red) and after (black) AFFINIMIP SPE 18 F Clean-up Protocol : Conditioning : Acetonitrile Loading : Water Acetonitrile or Water - DMSO Washing : Acetonitrile/Deionized Water Elution : Acetonitrile or Ethanol Format : Cartridge compatible with automates Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature Ordering information: Catalog number: Time (min) RP for 10 columns 32

33 intensity intensity AFFINIMIP SPE 18 F - Nucleophilic Fluoration AFFINIMIP SPE 18 F - Nucleophilic Fluoration are selective extraction solid phases used to retain specific tagbased compounds to purify rapidly and perfectly a 18 F radiotracer with no need of a preparative HPLC. AFFINIMIP SPE 18 F - Nucleophilic Fluoration PRINCIPLE: A label can be bound to any precursors submitted to nucleophilic radiofluoration. -OH + ClO 2 S- SO 3 The corresponding 18 F radiotracer obtained from the fluoration of this derivatized precursor is purified on AFFINIMIP SPE 18 F - Nucleophilic Fluoration cartridges which have strong interactions for labelled compounds. F -, Kryptofix Precursor SO F Pure Radiotracer 3 ACN or DMSO Example of Application: Proline Sulfonated precursors of radiotracers were labelled through the leaving group used for nucleophilic substitution. Then the fluorination was carried out in the presence of Kryptofix and KF in Acetonitrile at 95 C. The crude mixture was loaded on the SPE cartridge. Proline radiotracer was then isolated. O S O O O N O KF Kryptofix, ACN, 95 C Proline Precursor RP101-REA2 O OMe F O N OMe O O + -SO 3 - Proline Radiotracer SO 3 - O S O O O Proline Precursor N O O OMe O O N O O N Kryptofix O O F N O O O OMe Proline Radiotracer Time (min) SIM of Kryptofix, Proline precursor, Fluoro-proline and Sulfonic acid before purification on AFFINIMIP SPE 18 F. The LC-MS analysis shows that the elution fraction was constituted of the radiotracer (more than 95 % recoveries). No trace of the labeled precursor and of the labelled sulfonic acid was detected. Ordering information: Catalog number: Labeling agent ( ) RP101-REA1-10mg AFFINIMIP SPE 18 F - Nucleophilic Fluoration KIT RP101-01K for 10 columns AND 10mg of labeling agent Time (min) SIM of Kryptofix, proline precursor, Fluoro-proline and sulfonic acid after purification on AFFINIMIP SPE 18 F. Protocol : Conditioning: Acetonitrile, Water Loading: Water Acetonitrile or Water - DMSO Washing: Acetonitrile - Water Elution: Acetonitrile Format : Cartridge compatible with automates Particle diameter range : µm Stability : 3 years Shipping & Storage : Ambient temperature Related product: PROLINE Precursor RP101-REA2-10mg 33

34 AFFINIMIP SPE PRODUCT LIST Products Designation Definition Reference Nber of cartridges Amphetamines AFFINIMIP SPE Amphetamines Selective SPE cartridges for Amphetamines derivatives DG DG Bisphenol A AFFINIMIP SPE Bisphenol A Selective SPE cartridges for Bisphenol A (PP) Selective SPE cartridges for Bisphenol A (Glass) FS FS FS106-02G 25 FS106-03G 50 Carboxylic acids AFFINIMIP SPE Carboxylic acids Selective SPE cartridges for carboxylic acids compounds FS FS Catecholamines AFFINIMIP SPE Catecholamines 3mL Selective SPE cartridges for Catecholamines 1mL Selective SPE cartridges for Catecholamines DG DG DG100-02A 25 DG100-03A 50 Chloramphenicol AFFINIMIP SPE Chloramphenicol Selective SPE cartridges for Chloramphenicol FS110-02A 25 FS110-03A 50 Estrogens AFFINIMIP SPE Estrogens Selective SPE cartridges for Estrogens FS FS AFFINIMIP SPE GSH Selective SPE cartridges for Glutathione PH Glutathione (GSH) AFFINIMIP SPE GSH 96Well-Plate AFFINIMIP SPE Prep GSH Adducts 96 Well-plate for Glutathione PH W 1 Cartridges 10g resin for 10 to 15mL incubation solution at 5mM GSH Cartridges 30g resin for 15 to 50mL incubation solution at 5mM GSH PH PH Metanephrines AFFINIMIP SPE Metanephrines 3mL Selective SPE cartridges for Metanephrines 1mL Selective SPE cartridges for Metanephrines DG DG DG101-02A 25 DG101-03A 50 Ochratoxin A AFFINIMIP SPE Ochratoxin A 3mL Selective SPE cartridges for Ochratoxin A 6mL Selective SPE cartridges for Ochratoxin A in PEPPER FS FS FS101PP FS101PP Patulin AFFINIMIP SPE Patulin 6mL 100mg Selective SPE cartridges for Patulin 6mL 200mg Selective SPE cartridges for Patulin (for DRIED APPLE and higher enrichment with apple juice) FS FS FS mg 25 FS mg 50 AFFINIMIP SPE Patulin & Pectinase kit Kit of selective SPE cartridges for Patulin + 50mL Pectinase enzyme solution FS102-02K 25 FS102-03K 50 34

35 AFFINIMIP SPE PRODUCT LIST Products Designation Definition Reference Number of cartridges Pectinase 50 ml Pectinase enzyme solution REA mL Phenolics Tamoxifen Zearalenone Zearalenone and Fumonisins Zeranol Residues AFFINIMIP SPE Phenolics AFFINIMIP SPE Tamoxifen AFFINIMIP SPE Zearalenone AFFINIMIP SPE FumoZON AFFINIMIP SPE Zeranol Residues Selective SPE cartridges for Phenolic compounds FS Selective SPE cartridges for Tamoxifen PH Selective SPE cartridges for ZON Selective SPE cartridges for Zearalenone and Fumonisins Selective SPE cartridges for Zeranol Residues FS FS FS FS FS FS AFFINIMIP SPE 18 F-Aromatic Nucleophilic Substitution SPE cartridges compatible with automates for purification of radiotracers issued from Aromatic Nucleophilic Substitution RP F radiotracer AFFINIMIP SPE 18 F- Nucleophilic Fluoration KIT of 10 SPE cartridges compatible with automates for purification of radiotracers issued from Nucleophilic Fluoration + 10mg of labeling agent 10 mg of labeling agent RP101-01K RP101- REA1-10mg 10mg of PROLINE Precursor RP101- REA2-10mg For other formats, please contact us POLYINTELL can provide you with other formats than the one described in the product list. Other formats available on demand and with an adapted protocol can be : different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc ) 96 well plates, HPLC columns, Preparative HPLC columns the format adapted to your application and your automate 35

36 AttractSPE TM POLYMERIC-BASED PRODUCTS FOR SPE 36

37 POLYMERIC AttractSPE TM CARTRIDGES Polymeric Solid-Phase Extraction For enhanced purification Pioneer in Molecularly Imprinted Polymers (MIP) and expert in polymer chemistry, POLYINTELL develops, manufactures and markets products for solid phase extraction applications. POLYINTELL has developed the last generation of polymeric SPE columns (AttractSPE TM ). AttractSPE TM columns and 96-well Plates are sample preparation products for the extraction of compounds from complex matrices. These cartridges contain sorbents which support the six common retention mechanisms used in today s analytical laboratory: Hydrophilic Lipophilic Balance (AttractSPE TM W/O) Mixed-mode Strong Cation Exchange (AttractSPE TM SCX) Mixed-mode Weak Cation Exchange (AttractSPE TM WCX) Mixed-mode Strong Anion Exchange (AttractSPE TM SAX) Mixed-mode Weak Anion Exchange (AttractSPE TM WAX) Reversed phase (AttractSPE TM DVB) AttractSPE TM cartridges provide all the advantages of polymeric sorbents, including robustness, simplified method development, wide applicability and are not affected by drying out. The combination of the water-wettable optimised surface chemistry, high surface area and ph stability ensures high reproducible recoveries for a wide range of analytes. 37

38 SELECTION OF THE AttractSPE TM CARTRIDGES AttractSPE TM polymeric sorbents have equivalent properties than the polymeric sorbents supplied by other manufacturers. This table gives the correspondence between AttractSPE TM and main manufacturers. POLYINTELL AttractSPE TM W/O AttractSPE TM SCX AttractSPE TM WCX AttractSPE TM SAX AttractSPE TM WAX AttractSPE TM DVB WATERS Oasis HLB Oasis MCX Oasis WCX Oasis MAX Oasis WAX PHENOMENEX Strata-X Strata-X-C Strata-X-CW Strata-X-A Strata-X-AW The choice of the suitable AttractSPE TM columns can be done by using the following method: -Determination of the nature of the analyte (neutral, acid, base) -Determination of the pka -Choice of the AttractSPE TM columns by using the following chart and application of the general protocol described on the instruction sheet - Analysis of the recovery yields AttractSPE TM WAX Strong acids pka<1 AttractSPE TM SCX Bases pka 2-10 AttractSPE TM DVB Neutral hydrophobic AttractSPE TM W/O Neutral AttractSPE TM SAX Acids pka 2-8 AttractSPE TM WCX Strong Bases pka >10 Sample preparation Conditioning and equilibration loading of the solution Washing with Formic acid 2% 2mL Methanol 2mL 5% NH4OH /Methanol Sample preparation Conditioning and equilibration loading of the solution Washing with Methanol/ Water 2mL Methanol Neutral compounds Sample preparation Conditioning and equilibration loading of the solution Washing with NH4OH 5% 2mL Methanol 2mL 5% Formic acid /Methanol Strong acids Bases Acids Strong bases 38

39 Recovery yield (%) n=5 POLYMERIC AttractSPE TM CARTRIDGES AttractSPE TM W/O AttractSPE TM W/O is an uncharged Hydrophilic and Lipophilic sorbent interacting with both, hydrophilic and hydrophobic interactions. AttractSPE TM W/O particularly suits for the extraction of a wide range of analytes (polar, apolar, neutral, acid, basic ) Protocol : Loading : Pre-treated sample Washing: Methanol - Water Elution: Methanol Format : 3mL cartridges Particle diameter range : 40 µm Pore size: 70 Å Surface area: 800 m 2 /g Stability : 3 years Shipping & Storage : Ambient temperature Comparative results of AttractSPE TM W/O vs a competitor s product Extraction of Procainamide and Acetaminophen (Cartridge of 3mL) 120 3mL cartridges AttractSPE W/O Competitor Procainamide Acetaminophen For further comparative results between our products and competitors, please contact us at contact@polyintell.com AttractSPE TM DVB Reversed phase SPE for extraction of hydrophobic analytes AttractSPE TM DVB is a polystyrene-divinylbenzene copolymer presenting a high hydrophobicity used as a reversed-phase. AttractSPE TM DVB particularly suits for the extraction of hydrophobic analytes. Format : 3mL cartridges Particle diameter range : 40 µm Pore size: 60 Å Surface area: 600 m 2 /g Stability : 3 years Shipping & Storage : Ambient temperature Ordering information: References and formats on the product list p 42 39

40 POLYMERIC AttractSPE TM CARTRIDGES AttractSPE TM WAX Mixed-mode SPE for extraction of strong acid analytes AttractSPE TM WAX is a weak anion exchange sorbent interacting with the analytes via a mixed mode retention mechanism, ion exchange with weak basic functional groups and reversed phase. AttractSPE TM WAX particularly suits for the extraction of strong acids. Protocol : Loading : Neutral to Acidified sample Washing 1: Formic acid Water Washing 2 or Elution 1: Methanol Elution 2: Ammonium hydroxide Methanol Format : 3mL cartridges Pore size: 60 Å Surface area: 650 m 2 /g Ionic capacity: 0.5 meq/g Particle diameter range : 40 µm Stability : 3 years Shipping & Storage : Ambient temperature AttractSPE TM WCX Mixed-mode SPE for extraction of strong basic analytes AttractSPE TM WCX is a weak cation exchange sorbent interacting with the analytes via a mixed mode retention mechanism, ion exchange with weak acid functional groups and reversed phase. AttractSPE TM WCX particularly suits for the extraction of strong bases and quaternary amines. Protocol : Loading: Neutral to Basic sample Washing 1: Ammonium hydroxide - Water Washing 2: Methanol Elution: Formic acid - Methanol Format : 3mL cartridges Pore size: 70 Å Surface area: 850 m 2 /g Ionic capacity: 0.77meq/g Particle diameter range : 40 µm Stability : 3 years Shipping & Storage : Ambient temperature Ordering information: References and formats on the product list p 42 40

41 POLYMERIC AttractSPE TM CARTRIDGES AttractSPE TM SAX Mixed-mode SPE for extraction of weak acid analytes AttractSPE TM SAX is a strong anion exchange sorbent interacting with the analytes via a mixed mode retention mechanism, ion exchange with strong basic functional groups and reversed phase. AttractSPE TM SAX particularly suits for the extraction of weak acids. Protocol : Loading: Neutral to Basic sample Washing 1: Ammonium hydroxide - Water Washing 2: Methanol Elution: Formic acid - Methanol Format : 3mL cartridges Particle diameter range : 40 µm Pore size: 60 Å Surface area: 600 m 2 /g Ionic capacity: 0.3 meq/g Stability : 3 years Shipping & Storage : Ambient temperature AttractSPE TM SCX Mixed-mode SPE for extraction of weak basic analytes AttractSPE TM SCX is a strong cation exchange sorbent interacting with the analytes via a mixed mode retention mechanism, ion exchange with strong acid functional groups and reversed phase. AttractSPE TM SCX particularly suits for the extraction of weak bases. Protocol : Loading : Neutral to Acidified sample Washing 1: Formic acid - Water Washing 2 or Elution 1: Methanol Elution 2: Ammonium hydroxide Methanol Format : 3mL cartridges Pore size: 60 Å Surface area: 600 m 2 /g Ionic capacity: 1meq/g Particle diameter range : 40 µm Stability : 3 years Shipping & Storage : Ambient temperature Ordering information: References and formats on the product list p 42 41

42 POLYMERIC AttractSPE TM PRODUCT LIST Format cartridges, of sorbent of amount Nber /box AttractSPE TM W/O AttractSPE TM SCX AttractSPE TM WCX AttractSPE TM SAX AttractSPE TM WAX AttractSPE TM DVB 1mL, 30mg 100 HLB-100.S.1.30 SCX-100.S.1.30 WCX-100.S.1.30 SAX-100.S.1.30 WAX-100.S.1.30 DVB-100.S mL, 60mg 50 HLB-50.S.3.60 SCX-50.S.3.60 WCX-50.S.3.60 SAX-50.S.3.60 WAX-50.S.3.60 DVB-50.S mL, 200mg 25 HLB-25.S SCX-25.S WCX-25.S SAX-25.S WAX-25.S DVB-25.S HLB-50.S SCX-50.S WCX-50.S SAX-50.S WAX-50.S DVB-50.S wells Plate, 30mg 1 HLB-1.96W.30 SCX-1.96W.30 WCX-1.96W.30 SAX-1.96W.30 WAX-1.96W.30 DVB-1.96W.30 Reversible (0.7mL) 25 HLB-25.REV.1 SCX-25.REV.1 WCX-25.REV.1 SAX-25.REV.1 WAX-25.REV.1 DVB-25.REV.1 50 HLB-50.REV.1 SCX-50.REV.1 WCX-50.REV.1 SAX-50.REV.1 WAX-50.REV.1 DVB-50.REV.1 Reversible (2mL) 10 HLB-10.REV.2 SCX-10.REV.2 WCX-10.REV.2 SAX-10.REV.2 WAX-10.REV.2 DVB-10.REV.2 25 HLB-25.REV.2 SCX-25.REV.2 WCX-25.REV.2 SAX-25.REV.2 WAX-25.REV.2 DVB-25.REV.2 For other formats, please contact us POLYINTELL can provide you with other formats than the one described in the product list. Other formats available on demand and with an adapted protocol can be : different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc ) 96 well plates, HPLC columns, Preparative HPLC columns the format adapted to your application and your automate 42

43 SiliaPrep SILICA-BASED PRODUCTS FOR SPE 43

44 PRODUCT SELECTION GUIDE BY MANUFACTURER SiliaPrep products are Silica-based sorbents SPE cartridges available in different formats including SPE cartridges and 48- & 96-well plates, with different sorbents, and in bed weights up to 10 grams (>10 g are also available in SiliaSep OT formats) SiliaPrep SPE cartridges have equivalent products supplied by other manufacturers. This table gives the correspondence between SiliaPrep and main manufacturers. Manufacturer Our Product SiliaPrep Waters Sep-Pak Phenomenex Strata NON POLAR SORBENTS C18 (17%) SiliaPrep C18 R31930B tc18 C18-E C18 WPD SiliaPrep C18 WPD R33229G C18 C18-T C8 SiliaPrep C8 R31030B C8 C8 POLAR SORBENTS Silica SiliaPrep Silica R10030B Silica Silica (Si-1) Cyano Diol nec Florisil Florisil PR Alumina Acidic Alumina Neutral Alumina Basic ION EXCHANGE SORBENTS SAX nec SCX WAX Diamine (WAX-2) WCX SPECIAL SORBENTS Activated Carbon SiliaPrep Cyano R38030B SiliaPrep Diol nec R35030B SiliaPrep Florisil AUT-0014 SiliaPrep Florisil PR AUT-0015 SiliaPrep Alumina Acidic AUT-0053 SiliaPrep Alumina Neutral AUT-0055 SiliaPrep Alumina Basic AUT-0054 SiliaPrep SAX nec R66530B SiliaPrep SCX R60530B SiliaPrep WAX R52030B SiliaPrep Diamine (WAX-2) R49030B SiliaPrep WCX R70030B SiliaPrep WCX AUT-0110 Cyanopropyl Cyano (CN) non-endcapped Diol (endcapped) - Florisil Florisil (FL-PR) - Florisil (FL-PR) Alumina A - Alumina N Alumina-N (AL-N) Alumina B - Accell Plus QMA SAX non endcapped - SCX Aminopropyl NH 2 /WAX non endcapped PSA - Accell Plus CM WCX non-endcapped AC2-44

45 Silica-Based Sorbents Normal phases SiliaPrep Silica : Most polar sorbent It presents a slightly acidic character and is used to extract various compounds from non-polar solvents through hydrogen bonding. Sorbent Number : R10030B Silica type : 60 Å, 500 m 2 /g, µm SiliaPrep Cyano : Moderately polar sorbent It is used as a normal phase (less polar compared to silica). SiliaPrep Cyano particularly suits for the extraction of acidic, basic and neutral compounds from aqueous solutions. It is also used as a reversedphase (less hydrophobic than C8 and C18). Sorbent Number : R38030B Loading : 7 % C Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm SiliaPrep Diol nec : Moderately polar sorbent It presents neutral character used to extract polar compounds from non-polar solvents and structural isomers. Alternative to silica when the acidic character is problematic. Sorbent Number : R35030B Loading : 8 % C Endcapping : No Silica type : 60 Å, 500 m 2 /g, µm SiliaPrep Florisil and Florisil PR (MgO 3 Si) : Polar sorbent They present a basic character used to extract nonpolar to moderately polar compounds from nonpolar solvents. They particularly suit for the retention of chlorinated pesticides, polychlorinated biphenyl (PCB s) and polysaccharides due to the magnesium ion. Sorbent Number : AUT-0014 and PKN- SPE-AUT-0015 (PR) Florisil type : µm Florisil PR type : µm SiliaPrep Alumina-Acidic, Neutral and Basic : Alumina can present either cationic, neutral and acidic character. It is used in a similar fashion as for the SiliaPrep Silica. The difference is that Alumina is more stable at high ph than silica. SiliaPrep Alumina particularly suit for the retention of aromatic compounds, aliphatic amines and compounds containing electronegative functions. Sorbent Number : AUT-0053 (Acidic), AUT-0054 (Neutral) and AUT-0055 (Basic) Alumina type : 60 Å, 0.9 g/ml, µm 45 Ordering information: References and formats on the product list p 48

46 Silica-Based Sorbents Reversed phases SiliaPrep C18 : Strongly hydrophobic and non-polar sorbent It was recently developed as an innovative C18 phase characterized by a homogeneous coverage of the silane on the surface. SiliaPrep C18 particularly suits for the extraction of acidic, neutral and basic compounds from aqueous solutions, various organic compounds from water, and drugs and metabolites from physiological fluids. Sorbent Number : R31930B Loading : 17 % C Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm SiliaPrep C18 nec : Strongly hydrophobic and nonpolar sorbent SiliaPrep C18 nec presents higher retention and polar selectivity for basic compounds than Sorbent C18. SiliaPrep C18 nec particularly suits for soft condition catch and release purification of glucoronides thanks to unreacted surface OH groups. Sorbent Number : R35530B Loading : 17 % C Endcapping :No Silica type : 60 Å, 500 m 2 /g, µm SiliaPrep C18 WPD : Strongly hydrophobic, nonpolar and high-loading capacity sorbent SiliaPrep C18 WPD is similar to C18 but can accomodate larger molecules and untreated matrices. SiliaPrep C8 and C8 nec : Moderately hydrophobic and non-polar sorbent Sorbent C8 and C8 nec are more selective than Sorbent C18 for big compounds such as PAH, vitamin D, and oils as well as greasy compounds. SiliaPrep C8 and C8 nec particularly suit for the extraction of extremely non-polar compounds. Sorbent Number : R33229G Loading : 13 % C Endcapping :Yes Silica type : 125 Å, 300 m 2 /g, µm Sorbent Number : R31030B and R31130B (nec) Loading : 12 % C Endcapping : Yes (R31030B) and No (R31130B) Silica type : 60 Å, 500 m 2 /g, µm SiliaPrep Phenyl : Moderately hydrophobic and non-polar sorbent it particularly suits for the extraction of non-polar compounds with different selectivities through π-π interactions including aromatic compounds and other non-polar phases. Sorbent Number : R34030B Loading : 9 % C Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm 46 Ordering information: References and formats on the product list p 48

47 Silica-Based Sorbents Ion Exchange and Mixed Mode phases SiliaPrep SAX (TMA Chloride) : Strong anion exchanger silica-based sorbent It is positively charged under all conditions. SiliaPrep SAX particularly suits for the extraction of acidic molecules (pka 3-5) SiliaPrep SAX-2 nec (TMA Acetate) : Strong anion exchanger silica-based sorbent SiliaPrep SAX-2 has a low-selectivity acetate counter anion already in place and particularly suits for the retention of acidic compounds with pkas < 5, such as carboxylic acids. SiliaPrep WCX (Carboxylic Acid) : Weak cation exchanger silica-based sorbent It particularly suits for the extraction of strong basic compounds (pka >9) SiliaPrep WAX (Amine) : Weak anion exchanger silica-based sorbent SiliaPrep WAX avoids irreversible retention of acidic molecules (pka < 3) and particularly suits for the separation of peptides, drugs and metabolites from physiological fluids, poly- and monosaccharides and structural isomers. SiliaPrep SCX (Tosic Acid) and SCX-2 (PropylSulfonic Acid) : Strong cation exchanger silica-based sorbent SiliaPrep SCX is slightly more acidic than the other. SiliaPrep SCX and SCX-2 particularly suit for amine «catch and release» purification. SiliaPrep Carbonate : General base quencher SiliaPrep Carbonate is the silica-bound equivalent of tetramethyl ammonium carbonate and is used as a general base to quench a reaction, free base amines in their ammonium salt form and to scavenge acids, boronic acids and acidic phenols including HOBt. SiliaPrep C8/Tosic Acid (C8/SCX) and C8/Propylsulfonic Acid (C8/SCX-2) particularly suits for the extraction of basic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids. SiliaPrep Tosic Acid/TMA chloride (SCX-2/SAX) particularly suits for the separation of acidic and basic molecules from non-ionizable molecules. Sorbent Number : R66530B Loading : 1.1 mmol/g Endcapping : No Silica type : 60 Å, 500 m 2 /g, µm Sorbent Number : R66430B Endcapping : No Silica type : 60 Å, 500 m 2 /g, µm Sorbent Number : R70030B Loading : 1.4 mmol/g Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm Sorbent Number : R52030B Loading : 1.6 mmol/g Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm Sorbent Number : 60530B (SCX) and R51230B (SCX-2) Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm Sorbent Number : R66030B Sorbent Number : R023830B (C8/SCX) and R028030B (C8/SCX-2) Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm Sorbent Number : R802830B Endcapping : Yes Silica type : 60 Å, 500 m 2 /g, µm 47 Ordering information: References and formats on the product list p 50

48 SiliaPrep SILICA-BASED SPE CARTRIDGES Product List Format of cartridge Amount of sorbent 1mL, 50mg 1mL, 100mg 3mL, 200mg 3mL, 500mg 6mL, 500mg 6mL, 1g 6mL, 2g #/box NON POLAR SORBENTS C18 nec (23%) R30130B-01B R30130B-01C R30130B-03G R30130B-03P R30130B-06P R30130B-06S R30130B-06U C18 (17%) R31930B-01B R31930B-01C R31930B-03G R31930B-03P R31930B-06P R31930B-06S R31930B-06U C18 nec (17%) R35530B-01B R35530B-01C R35530B-03G R35530B-03P R35530B-06P R35530B-06S R35530B-06U C18 WPD R33229G-01B R33229G-01C R33229G-03G R33229G-03P R33229G-06P R33229G-06S R33229G-06U C8 R31030B-01B R31030B-01C R31030B-03G R31030B-03P R31030B-06P R31030B-06S R31030B-06U C8 nec R31130B-01B R31130B-01C R31130B-03G R31130B-03P R31130B-06P R31130B-06S R31130B-06U Cyclohexyl R61530B-01B R61530B-01C R61530B-03G R61530B-03P R61530B-06P R61530B-06S R61530B-06U Phenyl R34030B-01B R34030B-01C R34030B-03G R34030B-03P R34030B-06P R34030B-06S R34030B-06U POLAR SORBENTS Silica R10030B-01B R10030B-01C R10030B-03G R10030B-03P R10030B-06P R10030B-06S R10030B-06U Cyano R38030B-01B R38030B-01C R38030B-03G R38030B-03P R38030B-06P R38030B-06S R38030B-06U Diol nec R35030B-01B R35030B-01C R35030B-03G R35030B-03P R35030B-06P R35030B-06S R35030B-06U Florisil AUT B AUT C AUT G AUT P AUT P AUT S AUT U Florisil PR AUT B AUT C AUT G AUT P AUT P AUT S AUT U Alumina Acidic AUT B AUT C AUT G AUT P AUT P AUT S AUT U Alumina Neutral AUT B AUT C AUT G AUT P AUT P AUT S AUT U Alumina Basic AUT B AUT C AUT G AUT P AUT P AUT S AUT U 48

49 SiliaPrep SILICA-BASED SPE CARTRIDGES Product List 96 Well Plates 12mL, 2g 12mL, 5g 10mL, 200mg 10mL, 500mg 2mL, 50mg 2mL, 100mg 500mg 1000mg R30130B-12U R30130B-20X R30130B-10G R30130B-10P R30130B-B R30130B-C R30130B-P R30130B-S R31930B-12U R31930B-20X R31930B-10G R31930B-10P R31930B-B R31930B-C R31930B-P R31930B-S R35530B-12U R35530B-20X R35530B-10G R35530B-10P R35530B-B R35530B-C R35530B-P R35530B-S R33229G-12U R33229G-20X R33229G-10G R33229G-10P R33229G-B R33229G-C R33229G-P R33229G-S R31030B-12U R31030B-20X R31030B-10G R31030B-10P R31030B-B R31030B-C R31030B-P R31030B-S R31130B-12U R31130B-20X R31130B-10G R31130B-10P R31130B-B R31130B-C R31130B-P R31130B-S R61530B-12U R61530B-20X R61530B-10G R61530B-10P R61530B-B R61530B-C R61530B-P R61530B-S R34030B-12U R34030B-20X R34030B-10G R34030B-10P R34030B-B R34030B-C R34030B-P R34030B-S R10030B-12U R10030B-20X R10030B-10G R10030B-10P R10030B-B R10030B-C R10030B-P R10030B-S R38030B-12U R38030B-20X R38030B-10G R38030B-10P R38030B-B R38030B-C R38030B-P R38030B-S R35030B-12U R35030B-20X R35030B-10G R35030B-10P R35030B-B R35030B-C R35030B-P R35030B-S AUT U AUT X AUT G AUT P AUT B AUT C AUT P AUT S AUT U AUT X AUT G AUT P AUT B AUT C AUT P AUT S AUT U AUT X AUT G AUT P AUT B AUT C AUT P AUT S AUT U AUT X AUT G AUT P AUT B AUT C AUT P AUT S AUT U AUT X AUT G AUT P AUT B AUT C AUT P AUT S 49

50 SiliaPrep SILICA-BASED SPE CARTRIDGES Product List Format of cartridge Amount of sorbent 1mL, 50mg 1mL, 100mg 3mL, 200mg 3mL, 500mg 6mL, 500mg 6mL, 1g 6mL, 2g #/box ION EXCHANGE SORBENTS SAX nec R66530B-01B R66530B-01C R66530B-03G R66530B-03P R66530B-06P R66530B-06S R66530B-06U SAX-2 nec R66430B-01B R66430B-01C R66430B-03G R66430B-03P R66430B-06P R66430B-06S R66430B-06U SCX R60530B-01B R60530B-01C R60530B-03G R60530B-03P R60530B-06P R60530B-06S R60530B-06U SCX-2 R51230B-01B R51230B-01C R51230B-03G R51230B-03P R51230B-06P R51230B-06S R51230B-06U WAX R52030B-01B R52030B-01C R52030B-03G R52030B-03P R52030B-06P R52030B-06S R52030B-06U Diamine (WAX-2) R49030B-01B R49030B-01C R49030B-03G R49030B-03P R49030B-06P R49030B-06S R49030B-06U WCX R70030B-01B R70030B-01C R70030B-03G R70030B-03P R70030B-06P R70030B-06S R70030B-06U MIXED-MODE and SPECIAL SORBENTS C8/SCX R023830B-01B R023830B-01C R023830B-03G R023830B-03P R023830B-06P R023830B-06S R023830B-06U C8/SCX-2 R028030B-01B R028030B-01C R028030B-03G R028030B-03P R028030B-06P R028030B-06S R028030B-06U SCX/SAX R802830B-01B R802830B-01C R802830B-03G R802830B-03P R802830B-06P R802830B-06S R802830B-06U Carbonate R66030B-01B R66030B-01C R66030B-03G R66030B-03P R66030B-06P R66030B-06S R66030B-06U Activated Carbon AUT B AUT C AUT G AUT P AUT P AUT S AUT U 50

51 SiliaPrep SILICA-BASED SPE CARTRIDGES Product List 96 Well Plates 12mL, 2g 12mL, 5g 10mL, 200mg 10mL, 500mg 2mL, 50mg 2mL, 100mg 500mg 1000mg R66530B-12U R66530B-20X R66530B-10G R66530B-10P R66530B-B R66530B-C R66530B-P R66530B-S R66430B-12U R66430B-20X R66430B-10G R66430B-10P R66430B-B R66430B-C R66430B-P R66430B-S R60530B-12U R60530B-20X R60530B-10G R60530B-10P R60530B-B R60530B-C R60530B-P R60530B-S R51230B-12U R51230B-20X R51230B-10G R51230B-10P R51230B-B R51230B-C R51230B-P R51230B-S R52030B-12U R52030B-20X R52030B-10G R52030B-10P R52030B-B R52030B-C R52030B-P R52030B-S R49030B -12U R49030B -20X R49030B -10G R49030B -10P R49030B -B R49030B -C R49030B -P R49030B -S R70030B-12U R70030B-20X R70030B-10G R70030B-10P R70030B-B R70030B-C R70030B-P R70030B-S R023830B-12U R023830B-20X R023830B-10G R023830B-10P R023830B-B R023830B-C R028030B-12U R028030B-20X R028030B-10G R028030B-10P R028030B-B R028030B-C R802830B-12U R802830B-20X R802830B-10G R802830B-10P R802830B-B R802830B-C R66030B-12U R66030B-20X R66030B-10G R66030B-10P R66030B-B R66030B-C AUT U AUT X AUT G AUT P AUT B AUT C 51

52 MYCOTOXINS STANDARDS & SPE ACCESSORIES 52

53 MYCOTOXINS STANDARDS POLYINTELL proposes the following Mycotoxins standards for mycotoxins analyses : Mycotoxins are toxic byproducts of mold infestations which can contaminate food. To protect consumers from the health risks, most countries have adopted regulations to limit exposure to mycotoxins. Our mycotoxins standards are accurately prepared solutions of a mycotoxin or a mixture of mycotoxins to a determined concentration. These standards can be used for the implementation of the calibration curve as well as for the development of a new analytical methods. List of Mycotoxin Family proposed : Aflatoxins Ochratoxin Patulin Trichothecenes A and B Zearalenone Fumonisins Stability: 1 year Storage: 4 C MYCOTOXINS STANDARDS Product List Mycotoxines Aflatoxins Product Concentration (µg/ml) Volume Référence Aflatoxin B1 2 1mL REA-AFLB1-1ML Aflatoxin B mL REA-AFLB2-1ML Aflatoxin G1 2 1mL REA-AFLG1-1ML Aflatoxin G mL REA-AFLG2-1ML Mixture Aflatoxins: B1-B2-G1-G mL REA-MIXAFL-1ML Aflatoxin M mL REA-AFLM1-1ML Ochratoxin Ochratoxin A 100 1mL REA-OTA-1ML Patulin Patulin 100 1mL REA-PAT-1ML T2 Toxin 100 1mL REA-T2-1ML Trichothecenes A HT-2 Toxin 100 1mL REA-HT2-1ML Trichothecenes B Deoxynivalenol (DON) 100 1mL REA-DON-1ML Zearalenone Zearalenone 100 1mL REA-ZON-1ML Fumonisine B1 50 1mL REA-FB1-1ML Fumonisines Fumonisine B2 50 1mL REA-FB2-1ML Mixture Fumonisine: B1-B2 50 1mL REA-MIXFB-1ML 53

54 SPE ACCESSORIES POLYINTELL proposes the complete set of equipments required to carry out SPE experiments: Manifold ACC-MAN1 Like all chromatography techniques, Use of SPE cartridges needs a precise control of flow rate for maintaining reproducible extractions. Solid Phase extraction Vacuum Manifold allows you to control the flow and to process up to 12 (12-port version) or 24 (24-port version) AFFINIMIP SPE samples simultaneously, to gain significantly time during sample preparation steps. SPE Adapter & Reservoir kit ACC-AR1 Tube adapters serve to pile one SPE tube on top of another to provide different selectivities. A larger empty syringe barrel can be stacked on top of a smaller SPE tube to act as a larger load reservoir. Or, they can serve as an adapter for positive pressure methods (e.g. from a syringe or air/ N2 line). Mini-Vap ACC-VAP1 The 6-Port Mini-Vap concentrator/evaporator processes six vials at one time. The Mini-Vap includes a needle valve for fine metering of air or nitroge n drying gas. Mini PUMP ACC-PUMP Mini diaphragm vacuum pump for solid phase extraction experiments Portable 5.5L/min ~120 torr vacuum Oil-free portable Vacuum pump trap ACC-TRAP SPE Vacuum pump trap kit SPE ACCESSORIES Product list Installed between the manifold and the vacuum pump, it collects all liquids that are aspirated preventing contamination of the vacuum pump with a capacity of 1L. SPE Accessories Designation Definition Reference Manifold SPE Vaccum Manifold 12-port model ACC-MAN1 SPE Adapter & Reservoir kit SPE Adapter & Reservoir kit Kit of 12 reservoirs 60ml and adapters for use with 1,3 & 6 ml cartridges ACC-AR1 Mini-Vap Mini Evaporator/Concentrator 6 port Mini-Vap Evaporator/Concentrator for use with 1 to 250mL containers ACC-VAP1 Mini PUMP Mini vacuum pump Laboport diaphragm vacuum mini pump, 5.5L/min ACC-PUMP Vacuum pump trap SPE Vacuum pump trap kit 1L trap kit ACC-TRAP 54

55 CUSTOM-MADE PRODUCTS & SERVICES 55

56 CUSTOM-MADE PRODUCTS & SERVICES POLYINTELL offers full services for the design and synthesis of polymers complying with your specifications. With fully integrated technology platforms in organic, polymer and analytical chemistry, POLYINTELL has been partner in more than 40 projects and helps its customers by innovative solutions for their complexes challenges. POLYINTELL has developed a library of monomers giving a family of selective stationary phases based on its proprietary technology, which have shown a strong potential for the extraction, purification and detection of various compounds. SPE for analytical applications Detection kits development Custommade solutions for: SPE for radiopharma purifications Molecularly imprinted polymers development Did you know? Molecularly imprinted polymer (MIP) is a polymer with a «memory» of the shape and the functional groups of a target molecule. This material is designed in order to recognize selectively this molecule, even in the presence of compounds with structure and functionality similar to those of this molecule. High molecular recognition properties can be achieved with these MIPs for a variety of molecules and can be useful for its extraction, purification and detection. Why trusting POLYINTELL? POLYINTELL supplies The most comprehensive bank of sorbents From Silica to Polymers From conventional to very selective Reversed-phase, ionic exchange, MIPs etc Our Expertise on MIP, sample preparation, SPE protocol and detection kit development Quick and efficient development Reactivity 56

57 Feedback and optimisation PROJECT DEVELOPMENT No matter if we run a short term project (2-3 days) or long term project ( 4 to 6 months) we always follow a well-established procedure. The following scheme describes an example of a procedure for the development of a custom-made product based on our customer s requirements. After signing of Confidential Disclosure Agreements (CDA), key data are exchanged. A quotation based on our experience in separation science including a process sheet is developed. During the whole procedure, a permanent feedback is established with you. Step 1: We offer you a screening of our library which consists of several hundreds POLYINTELL phases to find the suitable one for your separation problem. The knowledge of the structure of the target substance, its functional groups and the solubility data give us valuable hints for the choice of the screening phases. Procedure for custom-made polymer phase Customer Step 2: For the selected phase, a protocol is implemented for your application. Then samples and / or prototypes are delivered to you for testing, evaluation and validation. Step 3: When the selected phase suits your application and has been validated, a scale-up is planned. A QC report is delivered with the product. The format of the product is correlated to your application and can be bulk material, SPE cartridges, HPLC columns etc STEP 1 STEP 2 Screening Elaboration of application protocol Delivery of samples/ prototypes to customer for validation STEP 3 Scale-up QC report Bulk material, SPE cartridges or HPLC columns If you need the development of new polymer for your application, please send us an to contact@polyintell.com or describe your needs using You can describe your application and our scientists will shortly evaluate your queries before contacting you as soon as possible. 57

58 PUBLICATIONS AND APPLICATION NOTES 58

59 LIST OF PUBLICATIONS AND APPLICATION NOTES Publications and litterature references: Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts detection, Analytica Chimica Acta, 672, 15 19, Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals, J. Chrom. A., 1217, , Solid-phase extraction using molecularly imprinted polymer for selective extraction of natural and synthetic estrogens from aqueous samples, Paolo Lucci, Oscar Núñez, M.T. Galceran, J. Chrom. A, 1218, , Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine, Tien Ping Lee, Bahruddin Saad, Wejdan Shakir Khayoon, Baharuddin Salleh, Talanta, 88, , Interest of molecularly imprinted polymers in the fight against doping. Extraction of tamoxifen and its main metabolite from urine followed by high-performance liquid chromatography with UV detection. J. Chromatography A, , 81 88, New technological tools for isolating and measuring growth promoting agents in edible tissues and biological fluids, Emmanuelle Bichon, Sandrine Rochereau, Ludivine Seree, Stéphanie Prevost, Fabrice Monteau, Bruno Le Bizec (LABERCA) Poster session RAFA Quantification of estrogens at ppt levels in bovine plasma by Molecularly Imprinted Solid Phase Extraction and GC- MS/MS analysis, Sandrine Rochereau, Emmanuelle Bichon, Frédérique Courant, Fabrice Monteau, Stéphanie Prévost, Flavia Hanganu, Nora Cesbron, Gaud Dervilly-Pinel, Bruno Le Bizec (LABERCA) Poster session, HTSP-2 and HTC Analysis of urinary neurotransmitters by capillary electrophoresis: Sensitivity enhancement using field-amplified sample injection and molecular imprinted polymer solid phase extraction, Bérengère Claude, Reine Nehmé, Philippe Morin, Analytica Chimica Acta, 699 (2), , More technical details and Application notes on available on our web site (): Application note - AFFINIMIP SPE 18 F Aromatic nucleophilic substitution Application note - AFFINIMIP SPE Amphetamines - Human serum and urine Application note - AFFINIMIP SPE Bisphenol A - Infant formula Application note - AFFINIMIP SPE Bisphenol A Powdered Infant formula Application note - AFFINIMIP SPE Bisphenol A - Beer Application note - AFFINIMIP SPE Bisphenol A Canned food Application note - AFFINIMIP SPE Bisphenol A Vegetable puree in plastic food packaging Application note - AFFINIMIP SPE Chloramphenicol Honey Application note - AFFINIMIP SPE Chloramphenicol Bovine Urine Application note - AFFINIMIP SPE GSH Application note - AFFINIMIP SPE FumoZON - Maize and Maize-based baby food Application note - AFFINIMIP SPE Metanephrine in plasma - comparison with WCX Application note - AFFINIMIP SPE Ochratoxin A - Cereals and Spices Application note - AFFINIMIP SPE Ochratoxin A - Red and White Wine Application note - AFFINIMIP SPE Ochratoxin A - Automated Protocol (GILSON GX271 ASPEC ) Application note - AFFINIMIP SPE Patulin - Apple Juice and Apple Puree Application note - AFFINIMIP SPE Patulin - Baby Food Application note - AFFINIMIP SPE Patulin - Cider Application note - AFFINIMIP SPE Patulin - Multifruit Puree Application note - AFFINIMIP SPE Patulin Dried apple Application note - AFFINIMIP SPE Zearalenone - Cereal Products and Baby Food Application note - AFFINIMIP SPE Zearalenone Automated Protocol (GILSON GX271 ASPEC ) Application note - AFFINIMIP SPE Zearalenone-Edible corn oil Patent portfolio Patents available upon request: WO07/004197; EP ; WO08/132384; WO09/056716; WO10/