TEST SPECIFICATION GUIDE

Transcription

1 TEST SPECIFICATION GUIDE The Test Specification Guide will be available to CML HealthCare CCC s / POCC s, and to all CML clients upon request (electronically and/or hard copy). This guide outlines the information needed to access the services provided by CML Healthcare for the procurement of laboratory specimens. Each individual test listing is arranged in a consistent format, providing specific information. This guide provides the following information: Test name, synonyms or other common names for the test and the computer testing code. Patient preparation, including patient care instruction prior to, or during specimen collection, or performance of the test. Patient clinical information that is required because of its relevance to the determination of the diagnosis, and to the testing protocol. The clinical information includes, but is not limited to, patient history, date of birth, sex, ethnic background, height and weight. Specimen collection instructions, including specimen type, container or vacutainer tube, specific days and times for sample procurement. Post specimen collection instructions including storage and transportation instructions, testing facility, estimated time for test results availability, and billing information. Unless specified otherwise, specimen storage and transport is at room temperature. TSG GENERAL INFORMATION Page 1 of 6 CML HealthCare Inc Test Specification Guide Version: Aug-2015 DOI: Sept/2005

2 SPECIMEN PROCESSING INFORMATION Tests are listed in the manual under the following headings: TEST: The test is listed first by its most common standard nomenclature and underneath any alternate names. Each test request is specifically cross-referenced. CODE: The test code(s) must always be Data Entered unless otherwise specified. SPECIMEN REQUIREMENT: Blood test requests are indicated as Serum, Plasma, or Blood. Instructions will specify either minimum volume required or centrifuge only. When a minimum volume amount is indicated, the vacutainer tube must be centrifuged, and an aliquot separated into a plastic transport tube. BILLING: All tests are considered OHIP or non-ohip payable. Tests indicated with OHIP are covered by OHIP and are patient payment exempt upon presentation of a valid Ontario Health Card. Tests indicated with a dollar amount after the test, require patient payment before specimen collection. TSG GENERAL INFORMATION Page 2 of 6 CML HealthCare Inc Test Specification Guide Version: Aug-2015 DOI: Sept/2005

3 LOC: The laboratory, which performs the test, is designated by a unique abbreviation. Abbreviation Testing Facility Testing Facility Phone # BAGL Bay Area Genetic Laboratory CENTO Centogene AG CML CML HealthCare CVH Credit Valley Hospital /4214 DYN Dynacare HLRC Hamilton Lab Reference Center HOSP Designated Hospital HRL Hemostasis Reference Laboratory x KGH Kingston General Hospital LHSC London Health Services Center ext LL Life Labs LLG LifeLabs Genetics MSH Mount Sinai Hospital MUMC McMaster University Medical Centre x NAT Natera Inc NYGH North York General Hospital OGH Oshawa General Hospital PHL Public Health Labs PLSI Phenomenome Lab Services Inc QUEST Quest Diagnostics Inc SBH Sunnybrook Health Science Centre SKH Hospital for Sick Kids SMH St. Michael s Hospital SJH St. Joseph s Hospital TGH Toronto General Hospital VTF Various Testing Facilities TSG GENERAL INFORMATION Page 3 of 6 CML HealthCare Inc Test Specification Guide Version: Aug-2015 DOI: Sept/2005

4 LOCATION INDEX ON REPORTS LOCATION NAME ADDRESS FACILITY CODE CML HEALTHCARE MAIN LABORATORY 6560 KENNEDY ROAD, MISSISSAUGA L5T 2X4 70 MOUNT SINAI HOSPITAL 600 UNIVERSITY AVENUE, TORONTO M5G 1X5 82 UNIVERSITY HEALTH NETWORK (TORONTO GENERAL SITE) 190 ELIZABETH AVENUE, TORONTO M5G 2C4 83 NATERA INC INDUSTRIAL ROAD, SAN CARLOS, CA NORTH YORK GENERAL HOSPITAL 4001 LESLIE STREET, TORONTO M2K 1E1 84 LAKERIDGE HEALTH CORPORATION 1 HOSPITAL COURT, OSHAWA L1G 2B9 85 CREDIT VALLEY HOSPITAL 2200 EGLINTON AVE. W., MISSISSAUGA L5M 2N1 86 SUNNYBROOK HEALTH SCIENCE CENTRE 2075 BAYVIEW AVENUE, TORONTO M4N 3M5 87 PUBLIC HEALTH LAB TORONTO BRANCH 81 RESOURCE ROAD, TORONTO M9P 3T1 90 DYNACARE 245 PALL MALL STREET, LONDON N6A 1P4 92 ST MICHEAL S HOSPITAL 30 BOND ST, TORONTO, ONT M5B 1W8 93 LIFE LABS 100 INTERNATIONAL BLVD, TORONTO M9W 6J6 94 HAMILTON LAB REFERENCE CENTRE 50 CHARLTON AVE. E., HAMILTON L8N 4A6 95 HEMOSTASIS REFERENCE LABORATORY 711 CONCESSION ST, 15(H) WING, 2 ND FL L8V 1C3 70 PHENOMENOME LABORATORY SERVICE INC. BAY AREA GENETIC LABORATORY CENTOGENE AG DOWNEY ROAD, SASKATOON, SASKATCHEWAN 205B-565 SANATORIUM ROAD, SIR WILLIAM OSLER BLDG, HAMILTON SCHILLINGALLEE 68, ROSTOCK, GERMANY S7N 4L8 L9C 7N QUEST DIAGNOSTICS INC. LENEXA RENNER BLVD., LENEXA, KS, USA TSG GENERAL INFORMATION Page 4 of 6 CML HealthCare Inc Test Specification Guide Version: Aug-2015 DOI: Sept/2005

5 LIST OF TESTS MIDWIVES ARE PERMITTED TO ORDER (TESTS FOR MOTHERS): CHEMISTRY/RIA HEMATOLOGY BACTERIOLOGY - Glucose - W.B.C differential count Antibiotic Sensitivity - Glucose Challenge, (includes R.B.C Morphology - Chlamydia Gestational Screen and platelet estimate) - Culture Cervical, - Urinalysis Routine - W.B.C (lkc count, excluding Vaginal (includes G.C) Chemical whole blood manual method) - Culture Other swabs - Urinalysis - Hematocrit or pus Microscopic - Hemoglobin examination - Sickle cell solubility test - Culture Urine - Estriol (screen) - Virus Isolation - HCG - Kleihauer - Wet preparation (for - Hepatitis associated antigen or antibody - Blood Group per antigen fungus, tricomonas, parasites) immunoassay - Strep B rapid screen - Alphafetoprotein Screen - Albumin Quantitative - Serum Ferritin - Serum Folate CYTOLOGY IMMUNOLOGY IMMUNOHEMATOLOGY - Cervicovaginal specimens - Pregnancy test - Virus antibodies hemagglutination inhibition or ELISA technique - Non-cultural, indirect antibody or antigen assays by fluorescence, agglutination or ELISA technique (toxoplasmosis) - HTLVIII/LAV antibody screen by ELISA technique (HIV Antibody) - Antibody Identification Incomplete antibody - Antibody screen - Blood group ABO and Rho (D) - Direct Anti-human globulin test - Direct Anti-human globulin test TSG GENERAL INFORMATION Page 5 of 6 CML HealthCare Inc Test Specification Guide Version: Aug-2015 DOI: Sept/2005

6 LIST OF TESTS MIDWIVES ARE PERMITTED TO ORDER (TESTS FOR NEWBORNS): CHEMISTRY/RIA HEMATOLOGY IMMUNOHEMATOLOGY - Bilirubin Total - W.B.C differential count - Blood group ABO and - Bilirubin Conjugated (includes R.B.C Rho (D) - Glucose Morphology and platelet - TSH/PKU Newborn estimate) screening - Platelet count - W.B.C (lkc count, excluding whole blood manual method) - Hematocrit - Hemoglobin LIST OF TESTS MIDWIVES ARE PERMITTED TO ORDER (TESTS FOR FATHERS/DONORS): CHEMISTRY/RIA HEMATOLOGY IMMUNOHEMATOLOGY - Urinalysis microscopic examination - Sickle cell solubility test (screen) - Blood group ABO and Rho (D) - Hepatitis associated antigen or antibody immunoassay - Blood group per antigen BACTERIOLOGY - Antibiotic Sensitivity - Chlamydia - Culture other swabs or pus - Virus isolation - Wet preparation (for fungus, trichomonas, parasites) IMMUNOLOGY - HTLVIII/LAV antibody screen by ELISA technique (HIV Antibody) TSG GENERAL INFORMATION Page 6 of 6 CML HealthCare Inc Test Specification Guide Version: Aug-2015 DOI: Sept/2005

7 3A/G RATIO (ALBUMIN/ GLOBULIN RATIO) A1C (GLYCOSYLATED HEMOGLOBIN) (HbA1C) (HEMOGLOBIN A1C) ABO, RhD (ABO & TYPE) (BLOOD GROUP & RhD) (BLOOD GROUP) (Rh TYPING) ABO, Rh(D), GENOTYPE (BLOOD GROUP, Rh(D) & GENOTYPE) (GENOTYPE) E.G. ANTIGENS C, E, c, e ABO & ANTIBODY SCREEN (ABO & SCREEN) (PRENATAL SCREEN) (TYPE & SCREEN) (BLOOD GROUP PRENATAL ANTIBODY) ACE (ANGIOTENSIN CONVERTING ENZYME) Refer to ALBUMIN/GLOBULIN RATIO Refer to HEMOGLOBIN A1C Refer to BLOOD GROUP Refer to BLOOD GROUP PHENOTYPE Refer to BLOOD GROUP and Refer to ANTIBODY SCREEN Refer to ANGIOTENSIN CONVERTING ENZYME ACETAMINOPHEN 079A Serum PLAIN RED OHIP HLRC (TYLENOL) Minimum Volume required: 2 ml Record time in hours that have elapsed between last dose and specimen collection. TAT 5 days ACETONE 002 Serum GOLD SST OHIP DYN (KETONES). Do not open tube Refrigerate during storage and transport. TAT 8 days ACETONE, QUALITATIVE Urine OHIP CML (KETONES QUALITATIVE) 10 ml random urine Submit in a YELLOW cap conical tube. ACETYLCHOLINE 9144 Serum GOLD SST UNINSURED HLRC RECEPTOR ANTIBODY TAT 30 days ACETYL CHOLINESTERASE 057R Red cells 2 LAVENDER OHIP DYN (RBC CHOLINESTERASE) Centrifuge tubes within 1-hour of collection Aliquot and discard plasma from lavender tubes Send red cells only Keep tubes together with an elastic Store and transport refrigerated TAT 7 days TEST SPECIFICATION GUIDE - SECTION A Page 1 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

8 ACETYLSALICYLIC ACID (ASA) (ASPIRIN) (SALICYLATE) Refer to SALICYLATE ACYLCARNITINE 9341 Centrifuge, separate into transfer tube GREEN UNINSURED HLRC (FRACTIONATION) and freeze immediately. Store and send frozen ACID FAST BACILLUS (AFB) (MYCOBACTERIA TUBERCULOSIS DETECTION) (T.B. CULTURE) (TUBERCULOSIS CULTURE) ACID PHOSPHATASE, PROSTATIC ACID PHOSPHATASE TOTAL ACTH (ADRENOCORTICOTROPIC HORMONE) (CORTICOTROPIN) Refer to MYCOBACTERIA TUBERCULOSIS DETECTION Refer to CORTICOTROPIN ACTIVATED PROTEIN C 9901 Plasma LIGHT BLUE UNINSURED HLRC RESISTANCE Minimum Volume required: 2 ml (APCR) Patient should not be on anticoagulant therapy FREEZE PLASMA AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines TAT 25 days ACUTE LEUKEMIA PHENOTYPING (LYMPHOCYTE MARKERS, T & B CELLS) (LYMPHOPROLIFERATIVE DISEASE PHENOTYPING) ACUTE RUBELLA (RUBELLA VIRUS ANTIBODY, IGM) Refer to LYMPHOCYTE MARKERS, T & B CELLS Refer to RUBELLA VIRUS ANTIBODY, IgM ADAMTS Both Red and Blue vacutainers are required. PLAIN RED N/C MUMC (THROMBOTIC THROMBOCYTOPENIC Centrifuge, separate serum and plasma AND LIGHT BLUE PURPURA) into separate transfer tubes and freeze both ASAP. Store and send frozen. FORM AVAILABLE ON CML WEBSITE ADENOVIRUS ANTIBODY SEROLOGY NO LONGER AVAILABLE ADENOVIRUS PCR 9068 Specimen must be sent on dry ice. LAVENDER UNINSURED SKH A completed molecular microbiology requisition must be sent with specimen. (See also Ministry of Health guidelines) FORM AVAILABLE ON CML WEBSITE TEST SPECIFICATION GUIDE - SECTION A Page 2 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

9 ADIPONECTIN Serum GOLD SST UNINSURED LL Patient must be fasting for min of 8 hours Allow 30 mins for sample clot. Spin and separate, aliquot into transfer tube. Store and ship refrigerated. TAT 14 days. FORM AVAILABLE ON CML WEBSITE ADH (ANTI DIURETIC HORMONE) (ADH VASOPRESSIN) (VASOPRESSIN) Refer to VASOPRESSIN ADRENAL ANTIBODIES 9904 Serum GOLD SST OHIP HLRC ADRENOCORTICOTROPIC HORMONE (ACTH) (CORTICOTROPIN) AFB (ACID FAST BACILLUS) (MYCOBACTERIA TUBERCULOSIS DETECTION) (T.B. CULTURE) (TUBERCULOSIS CULTURE) AGGLUTINATION REACTION SCREEN (COLD AGGLUTININS SCREEN) AIDS (HIV) (HIV 1 & 2 ANTIBODY SCREEN) (HIV SEROLOGY) AGA (ANTI GLIADIN ANTIBODY) (GLIADIN ANTIBODIES) ALA (AMINOLEVULINATE) (AMINO LEVULINIC ACID) Refer to CORTICOTROPIN Refer to MYCOBACTERIA TUBERCULOSIS DETECTION Refer to COLD AGGLUTININS SCREEN Refer to HIV 1 & 2 ANTIBODY SCREEN Refer to GLIADIN ANTIBODIES Refer to PROPHYRIN PRECURSORS ALANINE AMINO 223 Serum GOLD SST OHIP CML TRANSAMINASE (ALT) (SGPT) ALBUMIN 005 Serum GOLD SST OHIP CML ALBUMIN, QUALITATIVE Urine OHIP CML (PROTEIN, TOTAL QUALITATIVE) 10 ml random urine Submit in a YELLOW cap conical tube. TAT 2 days TEST SPECIFICATION GUIDE - SECTION A Page 3 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

10 ALBUMIN, URINE 005U 24-Hour Urine CLEAR OHIP CML 24-HOUR 1 x 6 ml aliquot (ALBUMIN, QUANTITATIVE URINE) Submit in a clear cap vacutainer (MICROALBUMIN, 24-HOUR) Label tube MICROALBUMIN No preservative Submit a separate sample for other urine tests. State total 24-hour volume on the OHIP requisition, on the specimen container and in Notes and Instructions. Retain a duplicate 90 ml sample in the fridge until test is reported. TAT 2 days ALBUMIN, URINE 005RU Urine CLEAR OHIP CML RANDOM 6 ml random urine (ALBUMIN, QUANTITATIVE URINE) Submit in a clear cap vacutainer (MICROALBUMIN, RANDOM) Label tube MICROALBUMIN Submit a separate sample for other urine tests. TAT 2 days ALBUMIN/GLOBULIN RATIO (A/G RATIO) ALCOHOLS (GC) 9242 Whole Blood GRAY OHIP HRLC Includes Methanol, Ethanol, Acetone, Isopropanol Do not open tube. Do not separate. Use iodine swab to cleanse venepuncture site. This test is not available for CCC use. This test is only for use at Kennedy Road for hospital patients TAT 4 days ALCOHOL, ETHYL (ETHANOL) Refer to ETHANOL ALCOHOL, ISOPROPYL (ISOPROPANOL) Refer to ISOPROPANOL ALCOHOL, METHYL (METHANOL Refer to METHANOL ALDOLASE ALDOSTERONE, 300 **This test is for hospital clients only. CCC staff should not use this code.** HOSPITAL ONLY Serum GOLD SST OHIP HLRC and aliquot to transfer tube. Ship frozen TAT 24 days TEST SPECIFICATION GUIDE - SECTION A Page 4 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

11 ALDOSTERONE, UPRIGHT 2616 Plasma LAVENDER OHIP LL Minimum Volume Required: 1.0 ml Collect in the morning before 10:00am Record on requisition Time Upright number of hours since the patient arose (to the nearest 0.5hrs) Minimum time before collection in UPRIGHT position (standing, walking or sitting) is 2 hours. If patient has been standing or walking, have them sit for 5-10 minutes before collection. Collect blood in Lavender (EDTA) tube. Mix thoroughly by gentle inversion. Centrifuge immediately and transfer an aliquot of plasma to a labeled tube, cap tightly and FREEZE at -20 o C. Store and ship frozen at -20 o C TAT 1 week ALDOSTERONE, URINE 300U 24-Hour Urine OHIP DYN 50 ml aliquot submit in a 90 ml white cap container No preservative Patient must be on normal sodium intake and not receiving diuretics for one week before urine sample is collected. State total 24-hour volume on the OHIP Requisition, on the specimen container and in Notes & Instructions. Retain a duplicate 50 ml sample in the freezer until test is reported. FREEZE URINE AND SEND FROZEN Refer to the General Information Pages for Specimen Processing & Transport Guidelines TAT 14 days ALKALINE PHOSPHATASE 191 Serum GOLD SST OHIP CML (PHOSPHATASE ALKALINE) (ALP) ALKALINE PHOSPHATASE 191 Serum 2 GOLD SST OHIP CML FRACTIONATION 192 Label 1 SST autochem (ALKALINE PHOSPHATASE Label 1 SST Alk. Phos. Fract. ISOENZYME) (PHOSPHATASE ALKALINE ISOENZYMES) Testing Includes Total Alkaline Phosphase TAT 4 days ALLERGIC ALVEOLITIS 9036 Serum GOLD SST OHIP HLRC (ALLERGIC LUNG) (FARMERS LUNG) Store and send frozen. Do not confuse with Avian Precipitins Includes M. Faeni and T Vulgaris. To order Allergic Lung Serology please order both Farmer s Lung Precipitins (SFAR) AND Aspergillus Precipitins (SASPP) TAT 30 days TEST SPECIFICATION GUIDE - SECTION A Page 5 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

12 ALLERGY TESTING See chart Serum GOLD SST UNINSURED HRL (ASIA) Min Volume Required: 1ml (SERUM ALLERGEN TEST) Centrifuge and aliquot. (ALLERGEN SPECIFIC IGE Store and ship refrigerated. ANTIBODY TEST) Be specific when free texting allergen name. (RAST) Can enter up to nine allergens on one accession. (ALLERGEN SPECIFIC IMMUNOASSAY) TAT 5 days NOTE: TAT for unlisted allergens is 4-6 weeks. Uncommon/unlisted allergens should be followed up by contacting the Pre-Analytical Department to ensure that testing can be done prior to accessioning. Ensure the requested allergen is for diagnostic use. Research allergens are not available. Test Name Test Code Allergy Testing-First Allergen Allergy Testing-Second Allergen Allergy Testing-Third Allergen Allergy Testing-Fourth Allergen Allergy Testing-Fifth Allergen Allergy Testing-Sixth Allergen Allergy Testing-Seventh Allergen Allergy Testing-Eighth Allergen Allergy Testing-Nineth Allergen TEST SPECIFICATION GUIDE - SECTION A Page 6 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

13 ALLERGY TESTING MIX See Chart Serum GOLD SST UNINSURED HRL Centrifuge and aliquot Store and ship refrigerated Can enter up to four allergen mixes on one accession. Eg: Tree mix, Food mix, Grass mix TAT 5 days Test Name Test Code Allergy Testing- Mix Allergy Testing- Mix Allergy Testing- Mix Allergy Testing- Mix ALPHA-1 ANTITRYPSIN 555 Serum GOLD SST OHIP CML TAT 2 days ALPHA-1 ANTITRYPSIN 9905 Serum GOLD SST UNINSURED HLRC PHENOTYPE Minimum volume required: 1 ml FREEZE SERUM AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines Note: Alpha-1 Antitryspin Phenotyping Analysis is only available if previously measured alpha-1 antitrypsin was <1.5 g/l or patient is first-degree relative or spouse of known individual. Request must specify previous alpha-1 antitrypsin result and relationship for testing to proceed TAT 60 days ALPHA 1 ACID 9923 Serum GOLD SST OHIP HLRC GLYCOPROTIEN Centrifuge and aliquot to transfer tube. ALPHA-2 MACROGLOBULIN 556 Serum GOLD SST OHIP HLRC Centrifuge Only. TAT 20 days ALPHA-2 PLASMIN INHIBITOR 9258 Plasma LIGHT BLUE UNINSURED HLRC (ALPHA 2 ANTIPLASMIN) Centrifuge and aliquot Platelet Poor Plasma To transfer tube. Freeze immediately. Store and ship frozen TAT 25 days TEST SPECIFICATION GUIDE - SECTION A Page 7 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

14 ALPHA FETOPROTEIN, 691 C Serum GOLD SST OHIP CML ONCOLOGY (AFP-ONCOLOGY) Specify if testing is tumor related Diagnosis must be indicated ALPHA FETOPROTEIN, 691 P Serum GOLD SST OHIP VTF PREGNANCY (AFP-PREGNANCY) For risk assessment of open neural tube defects Testing is recommended at 16 weeks gestation Completed "Maternal Serum Screen Form must be provided by ordering Physician. Indicate on the form "AFP ONLY" Results will be reported directly to the requesting Physician by the testing location. TAT 5 days ALT (ALANINE AMINO TRANSAMINASE) (SGPT) Refer to ALANINE AMINO TRANSAMINASE ALUMINUM, PLASMA 9355 Plasma ROYAL BLUE UNINSURED HLRC Min Volume Required: 2mL K2 EDTA Centrifuge and aliquot plasma into Aliquot tube. Separate and refrigerate As soon as possible. ALUMINUM, 24HR URINE hr urine ACID WASHED CONTAINER UNINSURED LL Patient must avoid chocolate, fruits, juice, beer, coffee, teas and antactids for containing aluminium 24 hours PRIOR to and during collection of 24 hour urine. 24 hour urine MUST be collected in ACID WASHED container Record total volume and transfer 20 ml of measured 24hr urine into A labelled sterile urine container and cap tightly Store and ship refrigerated. TAT 5 days ALUMINUM, RANDOM URINE Random Urine ACID WASHED CONTAINER UNINSURED LL Minimum voume: 10mL Patient must avoid gadolinium-based Contrast media 48 hours prior to collection Collect urine in a labelled sterile 90mL container and Transfer WITHOUT DELAY into a labelled ACID WASHED container. Store and ship refrigerated TAT 1-2 weeks TEST SPECIFICATION GUIDE - SECTION A Page 8 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

15 ALZ-ID 4105 Serum PLAIN RED UNINSURED PLSI (ALZHEIMER S DISEASE) Minimum volume: 1.0mL (ALZID) Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Transfer an aliquot of serum to a labelled tube, cap tightly Store and ship refrigerated at 2-8 o C TAT 1-2 weeks AMINOLEVULINATE AMETHOPTERIN (METHOTREXATE) Refer to PORPHYRIN PRECURSORS Refer to METHOTREXATE AMIKACIN Serum PLAIN RED OHIP HLRC Minimum Volume required: 1 ml PEAK 304AP Collect 'peak' specimen 30 minutes after IV infusion or 1-2 hours after IM injection by physician TROUGH 304AT Trough before IV / IM injection by physician Record time in hours that have elapsed between last dose and specimen collection. Refrigerate during storage and transport. AMIKACIN, RANDOM 304AR Serum PLAIN RED OHIP HLRC Minimum Volume required: 1 ml Specimens submitted as peak or trough are preferred; random orders should be avoided whenever possible. Store and ship refrigerated AMINO ACIDS (METABOLIC SCREEN) Refer to METABOLIC SCREEN AMINO ACIDS, QUANTITATIVE 013 Plasma GREEN OHIP HLRC (AMINO ACID FRACTIONATION) Minimum Volume required: 1 ml - with Heparin (PHENYLALANINE) Fasting specimen preferred State age of patient, (date of birth), and clinical diagnosis State if patient is on a special diet FREEZE PLASMA AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines. TAT 30 days AMINO ACIDS, QUANTITATIVE 013U AMINOGLYCOSIDES REFER TO METABOLIC SCREEN Amikacin, Gentamycin or Tobramycin. See individual listings. TEST SPECIFICATION GUIDE - SECTION A Page 9 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

16 AMINOPHYLLINE (THEOPHYLLINE) (UNIPHYL) Refer to THEOPHYLLINE AMIODARONE 9417 Plasma GREEN UNINSURED HLRC Minimum Volume required: 3 ml with Heparin Draw 1-hour prior to next dose TAT 20 days AMITRIPTYLINE 079AM Serum ROYAL BLUE OHIP DYN (ELAVIL) Minimum Volume required: 2 ml - No Additive Centrifuge and aliquot into serum tube Collect specimen hours after last dose Record time in hours that has elapsed between last dose and specimen collection. Refrigerate during storage and transport. Testing Includes Nortriptyline TAT 14 days AMMONIA (NH3) TESTING NO LONGER AVAILABLE AMOBARBITAL 9411 Serum PLAIN RED OHIP HLRC (AMYTAL) Minimum Volume required: 3 ml AMOBARBITAL 9412 Urine OHIP HLRC (AMYTAL) Minimum Volume required: 10 ml random urine Submit in a 90 ml orange cap container AMOEBIC ANTIBODY 9078 Do not centrifuge tube PLAIN RED N/C PHL (E. HISTOLYTICA SEROLOGY ANTIBODY) (ENTAMOEBA HISTOLYTICA ANTIBODY) REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM AMOEBIC DETECTION Stool N/C PHL (E. HISTOLYTICA) Collect two stool samples 1 st in ova and parasite container 2 nd in 90 ml container with orange lid REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM AMOXAPINE Serum - NO LONGER AVAILABLE AMPHETAMINE 078AM Urine OHIP CML 10 ml random urine Submit in a blue cap conical tube TAT 3 days TEST SPECIFICATION GUIDE - SECTION A Page 10 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

17 AMYLASE 018 Serum GOLD SST OHIP CML (DIASTASE) AMYLASE 018U 24-Hour Urine OHIP CML (DIASTASE) 10 ml aliquot submit in a white cap conical tube No preservative State total 24-hour volume on the OHIP Requisition, on the specimen container and in Notes & Instructions. Retain a duplicate 90 ml sample in the fridge until test is reported. Testing includes urine creatinine and total volume. TAT 2 day AMYLASE 018RU Urine OHIP CML (DIASTASE) 10 ml random urine Submit in a white cap conical tube. TAT 2 days AMYLASE FLUID 018FL Fluid PLAIN RED CONTRACT HLRC Minimum volume required: 1 ml This test is NOT available for CCC use. This test is only available at Kennedy Lab for hospital patients. TAT 10 days AMYLASE FRACTIONATION 018I Serum GOLD SST UNINSURED HLRC (AMYLASE ISOENZYME) Indicate clinical problem requiring analysis. TAT 45 to 60 days AMYTAL (AMOBARBITAL) ANA (ANF) (ANTI NUCLEAR ANTIBODY) (CENTROMERE ANTIBODY) (NUCLEAR ANTIBODIES) (SLE ANTIBODIES) ANAFRANIL (CLOMIPRAMINE) ANCA C (CYTOPLASMIC) (ANTI NEUTROPHIL CYTOPLASMIC ANTIBODY C) (NEUTROPHIL CYTOPLASMIC ANTIBODIES) ANCA p (PERINUCLEAR) (ANTI NEUTROPHIL CYTOPLASMIC ANTIBODIES P) ANDROGEN TESTICULAR (TESTOSTERONE) Refer to AMOBARBITAL Refer to NUCLEAR ANTIBODIES Refer to CLOMIPRAMINE Refer to NEUTROPHIL CYTOPLASMIC ANTIBODIES - C Refer to NEUTROPHIL CYTOPLASMIC ANTIBODIES - P Refer to TESTOSTERONE TEST SPECIFICATION GUIDE - SECTION A Page 11 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

18 ANDROSTENEDIONE 305 Serum PLAIN RED OHIP SKH Spin, separate and freeze Store and ship FROZEN TAT 21 days ANDROSTERONE ANF (ANA) (ANTI-NUCLEAR ANTIBODY) (CENTROMERE ANTIBODY) (NUCLEAR ANTIBODIES) (SLE ANTIBODIES) NO LONGER AVAILABLE Refer to NUCLEAR ANTIBODIES ANGIOTENSIN CONVERTING 9245 Serum GOLD SST UNINSURED HLRC ENZYME (ACE) Assay cannot be performed on a lipemic specimen Refrigerate during storage and transport. ANION GAP 053 Serum GOLD SST OHIP CML Hemolyzed specimens are unacceptable ANTABUSE NO LONGER AVAILABLE ANTIBODY SCREEN AND C176 Collect blood in PINK TOP tube (K2EDTA). PINK TOP OHIP LL BLOOD GROUP Collect a full tube. Mix thoroughly by gentle - PRENATAL inversion. Send entire tube. (INDIRECT COOMBS) Store and ship refrigerated at 2-8 o C. ADDITIONAL INFORMATION: TR#C176 includes TR#C171 and TR#C173 DO NOT CODE THESE SEPARATELY Please ensure that the patient completes the LifeLabs Prenatal Questionnaire and attach the complete document to the OHIP requisition. For prenatal patients data enter pre-reportable prompts as NA (not applicable) DO NOT SEPARATE TAT 2 days ANTIBODY SCREEN C173 Collect blood in PINK top tube (K2EDTA). PINK TOP OHIP LL (REPEAT ANTIBODY SCREEN) Collect a full tube. Mix thoroughly by gentle inversion. Send entire tube. Store and ship refrigerated at 2-8 o C. ADDITIONAL INFORMATION: This code is for repeat Antibody Screen and also includes Blood Group test code C171. DO NOT CODE test code C171 seperately. NOTE: Antibody Identification will be performed if the screen is positive (reflexive testing) DO NOT SEPARATE TAT 2 days TEST SPECIFICATION GUIDE - SECTION A Page 12 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

19 ANTI CARDIOLIPIN AB (ANTI PHOSPHOLIPID) (CARDIOLIPIN ANTOBIDES) ANTI-CCP ANTI dsdna ANTIBODY (ANTI-DNA) (ANTI DSDNA DOUBLE STRANDED AB) (DNA ds ANTIBODIES) ANTI DIURETIC HORMONE (ADH) (VASOPRESSIN) Refer to CARDIOLIPIN ANTOBIDES Refer to CYCLIC CITRULLINATED PEPTIDE ANTIBODIES Refer to DNA ds ANTIBODIES Refer to VASOPRESSIN ANTI ENA Refer to EXTRACTABLE NUCLEAR ANTIBODIES SCREEN (ENA ANTIBODY) (EXTRACTABLE NUCLEAR ANTIBODIES SCREEN) ANTI ENDOMYSIAL ANTIBODY (ENDOMYSIUM ANTIBODIES) ANTI EPIDERMAL ANTIBODY (ANTI-SKIN ANTIBODIES) (PEMPHIGUS/PEMPHIGOID ANTIBODIES) ANTI GLIADIN ANTIBODY (AGA) (GLIADIN ANTIBODIES) Refer to ENDOMYSIUM ANTIBODIES Refer to PEMPHIGUS/PEMPHIGOID ANTIBODIES Refer to GLIADIN ANTIBODIES ANTI GLOMERULAR Refer to GLOMERULAR BASEMENT MEMBRANE ANTIBODY BASEMENT MEMBRANE (GLOMERULAR BASEMENT MEMBRANE ANTIBODY) ANTI-GLUTAMIC ACID 9233 Serum GOLD SST OHIP HLRC DEHYDECARBOXYLASE Minimum Volume Required: 1mL (ANTI-GAD) Centrifuge and aliquot Store and ship frozen TAT - 34 days ANTI HISTONE (HISTONE ANTIBODIES) Refer to HISTONE ANTIBODIES ANTI HBs (HEPATITIS B IMMUNE STATUS) (HEAPTITIS B VIRUS SURFACE ANTIBODY) Refer to HEPATITIS B VIRUS SURFACE ANTIBODY ANTI INSULIN (INSULIN ANTIBODIES) ANTI INTRINSIC FACTOR (INTRINSIC FACTOR ANTIBODIES) ANTI JO 1 (JO-1 EXTRACTABLE NUCLEAR ANTIBODIES) Refer to INSULIN ANTIBODIES Refer to INTRINSIC FACTOR ANTIBODIES Refer to EXTRACTABLE NUCLEAR ANTIBODIES TEST SPECIFICATION GUIDE - SECTION A Page 13 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

20 ANTI LA (SS-B) (SS-B EXTRACTABLE NUCLEAR ANTIBODIES) Refer to EXTRACTABLE NUCLEAR ANTIBODIES ANTI-LKM ANTIBODY 9237 Serum GOLD SST OHIP HLRC (LKM ANTIBODY) (ANTI-LIVER KIDNEY MICROSOMAL Store and ship refrigerated ANTIBODIES) TAT 14 days ANTI-MICROSOMAL ANTIBODIES (MICROSOMAL ANTIBODIES) ANTI MITOCHONDRIAL ANTIBODY (ASMA) (ANTI-SMOOTH MUSCLE ANTIBODIES) (MITOCHONDRIAL ANTIBODIES) (SMA) (SMOOTH MUSCLE ANTIBODY) Refer to ANTI-THYROID PEROXIDASE Refer to MITOCHONDRIAL ANTIBODIES ANTIMONY, RANDOM URINE Urine UNINSURED LL Min volume: 20mL Ensure hands are washed and free of contamination. For industrial exposure collect at end of work shift. Store and ship refrigerated. TAT 10 days ANTI-MULLERIAN HORMONE 9590 Serum PLAIN RED UNINSURED LL (AMH) Minium volume required: 1 ml (ANTI OVARIAN HORMONE) Centrifuge and aliquot (MIS) Store and ship frozen. TAT 10 days ANTIMYOCARDIAL ANTIBODY Serum GOLD SST UNINSURED LL Allow blood to clot for 30mins at room temp. Centrifuge. Store and ship refrigerated. TAT 6 days ANTI NEUTROPHIL CYTOPLASMIC ANTIBODIES - C (c-anca - CYTOPLASMIC) ANTI NEUTROPHIL CYTOPLASMIC ANTIBODIES - P (p-anca PERINUCLEAR) ANTI NUCLEAR ANTIBODY (ANA) (ANF) (CENTROMERE ANTIBODIES) (NUCLEAR ANTIBODIES) (SLE ANTIBODIES) ANTI PANCREATIC ISLET CELLS ANTIBODY (PANCREATIC ISLET CELL ANTIBODIES) Refer to NEUTROPHIL CYTOPLASMIC ANTIBODIES - C Refer to NEUTROPHIL CYTOPLASMIC ANTOBIDIES - P Refer to NUCLEAR ANTIBODIES Refer to PANCREATIC ISLET CELL ANTIBODIES ANTI PARIETAL CELL TEST SPECIFICATION GUIDE - SECTION A Page 14 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

21 ANTIBODIES (PARIETAL CELL ANTIBODIES) ANTI PHOSPHOLIPID (ANTI-CARDIOLIPIN) (CARDIOLIPIN ANTIBODIES) ANTI-PLATELET ANTIBODIES (PLATELET ASSOCIATED ANTIBODIES) (PLATELET ANTIBODY SCREEN) ANTI RETICULIN ANTIBODY (ANTI-RETICULIN AB) (RETICULIN ANTIBODIES) ANTI RNP ANTI RO (SS A) ANTI SCL 70 (Scl-70 ANTIBODIES) (SCLERODERMAL ANTIBODY) ANTI-SM (ANTI SMITH) ANTI-SMOOTH MUSCLE ANTIBODIES (ANTI-MITOCHONDRIAL ANTIBODIES) (ASMA) (MITOCHONDRIAL ANTIBODIES) (SMA) (SMOOTH MUSCLE ANTIBODY) ANTI-SPERM ANTIBODIES (SPERM ANTIBODIES) ANTI-STREPTOCCAL HYALURONIDASE ANTIBODY (ASH) ANTI-STREPTOLYSIN O TITRE (ASOT) (STREPTOLYSIN O ANTIBODY) Refer to PARIETAL CELL ANTIBODIES Refer to CARDIOLIPIN ANTIBODIES Refer to PLATELET ANTIBODY SCREEN Refer to RETICULIN ANTIBODIES Refer to EXTRACTABLE NUCLEAR ANTIBODIES Refer to EXTRACTABLE NUCLEAR ANTIBODIES Refer to EXTRACTABLE NUCLEAR ANTIBODIES Refer to EXTRACTABLE NUCLEAR ANTIBODIES SCREEN Refer to MITOCHONDRIAL ANTIBODIES Refer to SPERM ANTIBODIES Refer to STREPTOLYSIN O ANTIBODY ANTI-THROMBIN III 373 Plasma LIGHT BLUE OHIP HLRC (ANTI-THROMBIN ASSAY) Minimum Volume required: 1 ml Patient should not be on anticoagulant therapy Includes both Functional and Immunological testing FREEZE PLASMA AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines TAT 20 days TEST SPECIFICATION GUIDE - SECTION A Page 15 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

22 ANTI-THYROID ANTIBODY HP16A Serum GOLD SST OHIP LL (ATA) Minimum Volume Required: 2mL (ANTI-THYROID ANTIBODIES) Collect blood in SST. Allow blood to (THYROID ANTIBODY) clot at room temperature for 30 mins (THYROID ANTIBODIES) and separate by centrifugation. (THYROID AUTOANTIBODIES) Store and ship refrigerated at 2-8 o C for (THYROID AUTOANTIBODY) up to 5 days. This testing includes Anti-Thyroid Peroxidase and Anti-Thyroglobulin This test is NOT the same as Thyroglobulin (9494) TAT 4 days ANTI-THYROGLOBULIN 327 Serum GOLD SST OHIP LL (ATG) (THYROGLOBULIN ANTIBODIES) Minimum Volume required: 1.0 ml Collect blood in SST tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Store and ship refrigerated at 2-8 o C for up to 7 days. TAT 4 days ANTI-THYROID PEROXIDASE 326 Serum GOLD SST OHIP LL (ANTI TPO) Min Volumne Required: 1.0mL (TPO) (ANTI-PEROXIDASE) Collect blood in SST tube. Allow blood (ANTI-MICROSOMAL) clot at room temperature for 30 mins (MICROSOMAL ANTIBODY) (MICROSOMAL ANTIBODIES) and separate by centrifugation. (THYROID PEROXIDASE ANTIBODY) Store and ship refrigerated at 2-8 o C for up to 5 days. NOTE: If physician orders Anti-Thyroid Peroxidase AND Anti-Thyroglobulin together, please key HP16A. TAT 4 days APCR (ACTIVATED PROTEIN C RESISTANCE) Refer to ACTIVATED PROTEIN C RESISTANCE APOLIPOPROTEIN A Serum GOLD SST UNINSURED LL (APO A1) Minimum Volume required: 1.0 ml Collect blood in SST tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation AS SOON AS POSSIBLE. Store and ship refrigerated at 2-8 o C. TAT 3 days TEST SPECIFICATION GUIDE - SECTION A Page 16 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

23 APOLIPOPROTEIN B 1977 Serum GOLD SST UNINSURED LL (APO B) Minimum Volume required: 1.0 ml Collect blood in SST tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation AS SOON AS POSSIBLE. Store and ship refrigerated at 2-8 o C. TAT 3 days APOLIPOPROTEIN-E Plasma LAVENDER UNINSURED SMH (LIPO QUANT) Minimum volume required: 7 ml Assay is performed on consultation basis only PHYSICIAN MUST CONTACT DR. CONNELLY At the Lipid Research Lab at St. Michael s Hospital, Toronto. (416) It is preferred that the patient fast a minimum of 12 hours. Test is not performed if Triglycerides is normal. Collect 4 lavender tubes and mix thoroughly. Centrifuge and separate within 4 hrs of collection Transfer all the plasma to a labelled tube Store and ship ALL tubes refrigerated. TAT 20 days APO PROTEIN a (LIPOPROTEIN a) Refer to LIPOPROTEIN a ARBOVIRUS ANTIBODIES 9080 Do not centrifuge tube PLAIN RED N/C PHL PHL recommends both acute and convalescent samples be taken 2 weeks apart. REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM ARSENIC, BLOOD 9279 Whole Blood ROYAL BLUE (K2EDTA) UNINSURED HLRC Min Volume Required: 4mL Do not centrifuge. Send entire tube. TAT 20 days ARSENIC, HAIR 9908 Hair UNINSURED HLRC Clip hair close to the nape of the neck from 6-8 different locations 0.2 gm hair required (approximately 2 teaspoons full) Bleaches and dyes may interfere Submit in a 90 ml container TAT 45 days TEST SPECIFICATION GUIDE - SECTION A Page 17 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

24 ARSENIC, NAIL 9909 Nails UNINSURED HLRC Clip nails from all fingers Patient must remove nail polish prior to collection Submit in a 90 ml container TAT 20 days ARSENIC, 24 HOUR URINE Hour Urine UNINSURED HLRC 15 ml aliquot submit in a 90 ml white cap container Avoid seafood consumption 5 days prior to collection. Inorganic arsenic will be performed if total is elevated. State total 24-hour volume on the OHIP Requisition, on the specimen container and in Notes & Instructions Retain a duplicate 50 ml sample in the fridge until test is reported. TAT 10 to 60 days ARSENIC, RANDOM URINE 9186 Urine UNINSURED HLRC 15 ml random urine Submit in a 90 ml orange cap container Avoid seafood consumption 5 days prior to collection. Inorganic arsenic will be performed if total is elevated. TAT 30 days ARSENIC, INORGANIC hour Urine ACID WASHED CONTAINER UNINSURED LL TOTAL 24 HOUR URINE 24 hour urine must be collected in an ACID WASHED container Avoid seafood consumption 72 hours prior to collection. Record total volume and transfer 20mL into 90mL container Store and ship refrigerated. For industrial exposure a random urine is recommended. Creatinine level is determined on all 24 hours urines to assess the Completeness of the 24 hour urine collection. TAT 10 days ARSENIC TOTAL, Urine ACID WASHED CONTAINER UNINSURED LL RANDOM URINE 10 ml random urine Submit in a 90 ml orange cap container Patient must avoid gadolinium based contrast media Used for MRI s 48 hours prior to collection. 90mL ACID WASHED container is required. Store and ship refrigerated. TAT 1-2 weeks ARTHROPOD IDENTIFICATION 9028 Send entire specimen in container N/C PHL (BUGS) (LICE) REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM TEST SPECIFICATION GUIDE - SECTION A Page 18 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

25 ARYLSULFATASE A WBC 9383 Whole Blood GREEN CONTRACT HICL (HOSP ONLY) Min volume required: 7mL - Heparinized Test not available for CCC use This test is only for use at the Kennedy lab for hospital patients Client must call Client Services Urgent Desk between 8:00am and 9:00am to arrange a pickup no later than 10:00am. Do not separate. Maintain at room temp. Immediately ship directly to HICL before 12:00 pm (noon) on the day of collection. Sample must be analysed within 12 hours of collection. ASA (ACETYSALICYLIC ACID) (ASPIRIN) (SALICYLATE) Refer to SALICYLATE ASCORBATE 019 Serum GOLD SST OHIP DYN (ASCORBIC ACID) Minimum Volume required: 2 ml (VITAMIN C) Protect from light by aliquoting into amber tube. FREEZE SERUM AND SEND FROZEN Freeze within 30 minutes of collection Refer to the General Information Page for Specimen Processing & Transport Guidelines TAT 14 days ASH (ANTI STREPTOCCAL HYALURONIDASE AB) ASMA (ANTI SMOOTH MUSCLE ANTIBODY) (ANTI-MITOCHONDRIAL ANTIBODY) (MITOCHONDRIAL ANTIBODIES) (SMA) (SMOOTH MUSCLE ANTIBODY) Refer to MITOCHONDRIAL ANTIBODIES ASOT (ANTI STREPTOLYSIN O TITRE) (STREPTOLYSIN O ANTIBODY) Refer to STREPTOLYSIN O ANTIBODY ASPARTATE AMINO 222 Serum GOLD SST OHIP CML TRANSAMINASE (AST) (SGOT) ASPERGILLUS ANTIBODY 9033 Do not centrifuge PLAIN RED N/C PHL REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM TAT 30 days TEST SPECIFICATION GUIDE - SECTION A Page 19 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

26 ASPIRIN (ACETYSALICYLIC ACID) (ASA) (SALICYLATE) Refer to SALICYLATE AST (ASPARTATE AMINO TRANSAMINASE) (SGOT) ATA (ANTI-THYROID ANTIBODY) (THYROID ANTIBODIES) ATIVAN (LORAZEPAM) AVENTYL (NORTRIPTYLINE) Refer to ASPARTATE AMINO TRANSAMINASE Refer to ANTI-THYROID ANTIBODY Refer to LORAZEPAM Refer to NORTRIPTYLINE AVIAN PRECIPITINS 9034 Serum PLAIN RED UNINSURED HLRC (BIRD FANCIER S DISEASE) Centrifuge, separate into transfer tube and refrigerate. Billed per each allergen. Budgie & Pidgeon done routinely: goose, chicken, duck, canary, cockatiel, parrot, turkey must be requested if clinically indicated. TAT 18 days TEST SPECIFICATION GUIDE - SECTION A Page 20 of 20 CML HealthCare Inc Test Specification Guide Version: Oct-2015

27 B CAROTENE (CAROTENE) Refer to CAROTENE B-TYPE NATRIURETIC 1562 Plasma LAVENDER UNINSURED LL PEPTIDE Minimum volume required: 1.0mL (BNP) Collect blood in Lavender top tube (EDTA). Mix thouroughtly by gentle inversion and separate by centrifugation WITHIN 2-4 hours of collection. Transfer an aliquot of plasma to a labelled tube, cap tightly and FREEZE at -20 o C. Store and ship FROZEN at -20 o C. TAT - 5 days B12 (VITAMIN B12) (COBALAMINS) B2 MICROGLOBULIN (BETA 2-MICROGLOBULIN) (MICROGLOBULIN) Refer to COBALAMINS Refer to BETA 2-MICROGLOBULIN BARBITURATES SCREEN 026U Urine OHIP CML 10 ml random urine Submit in a blue cap conical tube TAT 2 days BARTONELLA ANTIBODY 9011 Do not centrifuge tube PLAIN RED N/C PHL (CAT SCRATCH DISEASE) REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM TAT 3 weeks BCR-ABL 9382 Whole Blood LAVENDER CONTRACT HLRC (QUANTITATIVE PCR) Min volume required: 10ml (BCR/ABL) Test is NOT available for CCC use. Test is only for use at Kennedy Lab for Hospital patients. Download requisition at Form must be completed and submitted along with specimen and req. Ship within 24 hours. If required store overnight at 4 C TAT 33 days BENADRYL (DIPHENHYDRAMINE) BENCE JONES PROTEIN (IEP RANDOM URINE) (IMMUNOELECTROPHORESIS) (HEAVY AND LIGHT CHAINS) Refer to DIPHENHYDRAMINE Refer to PROTEIN ANALYSIS BENCE JONES PROTEIN BENZENE (PHENOL) TEST SPECIFICATION GUIDE - SECTION B Page 1 of 6 CML HealthCare Inc Test Specification Guide Version Oct-2015

28 BENZODIAZEPINE SCREEN 078BE Urine OHIP CML 10 ml random urine Submit in a blue cap conical tube TAT 2 days BENZTROPINE MESYLATE Urine UNINSURED LL 10 ml random urine Store and ship refrigerated TAT 3 days BERYLLIUM LYMPHOCYTE Whole Blood 4 tubes GREEN TOP UNINSURED LL PROLIFERATION FOR CONTRACT USE ONLY - Hepartinized Collect Mon-Wed only. DO NOT SHIP ON FRIDAY. Do not refrigerate or freeze. Specimen must arrive within 24 hours of collection. Store and ship room temp. Specimens sent by FEDEX to the Celevland Clinic TAT 2-3 weeks BERYLLIUM RANDOM URINE Urine UNINSURED LL Min volume required: 20ml Ensure that hands are washed and clothes are free of contamination. Store and ship refrigerated. For Industrial exposure collect specimen at the end of the work shift. A random urine test includes creatinine to be performed the the referred testing site. TAT 5-10 days BETA 2 GLYCOPROTIEN I IgG 9268 Serum PLAIN RED OHIP HLRC (BETA-2-GP-I IgG Centrifuge and aliquot to transfer tube. Store and ship frozen. TAT 33 days BETA 2 MICROGLOBULIN 9101 Serum GOLD SST UNINSURED HLRC (B2 MICROGLOBULIN) (MICROGLOBULIN) Refrigerate during storage and transport. TAT 25 days BETA 2 MICROGLOBULIN 9101RU Urine UINNSURED HLRC (B2 MICROGLOBULIN) (MICROGLOBULIN) 10 ml random urine Submit in a 90 ml orange cap container Ask patient to void (discard), then drink a glass of water - collect urine for submission one hour later FREEZE URINE AND SEND FROZEN TAT 25 days TEST SPECIFICATION GUIDE - SECTION B Page 2 of 6 CML HealthCare Inc Test Specification Guide Version Oct-2015

29 BETA hcg (BHCG) (HUMAN CHORIONIC GONADOTROPIN) (CHORIOGONADOTROPIN) Refer to CHORIOGONADOTROPIN BETA HYDROXYBUTYRATE 9248 Serum GOLD SST UNINSURED HLRC (BHBA) Centrifuge, separate into transfer tube. (3HBA) Freeze immediately. Store and send frozen. TAT 6 days BETA TRANSFERRIN 9352 Fluid STERILE CONTAINER UNINSURED HLRC Accept any container/tube received. Indicate source. Store and send frozen. Analysis includes Beta 1 Transferrin and Beta 2 Transferrin TAT 14 days BICARBONATE (CO 2) (CARBON DIOXIDE) Refer to CARBON DIOXIDE BILE ACID 9307 Serum GOLD SST UNINSURED HLRC Minimum Volume required: 1 ml 12 hour fast required FREEZE SERUM AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines. TAT 30 days BIO AVAILABLE TESTOSTERONE (TESTOSTERONE BIO AVAILABLE) BILIRUBIN Refer to TESTOSTERONE BIO AVAILABLE Refer to UROBILINOGEN BILIRUBIN, DIRECT 031 Serum GOLD SST OHIP CML (CONJUGATED BILIRUBIN) (BILIRUBIN GLUCURONIDATED) BILIRUBIN, INDIRECT (UNCONJUGATED BILIRUBIN) (BILIRUBIN NON-GLUCURONIDATED) BILIRUBIN, TOTAL 030 Serum GOLD SST OHIP CML TEST SPECIFICATION GUIDE - SECTION B Page 3 of 6 CML HealthCare Inc Test Specification Guide Version Oct-2015

30 BIQUIN (Q-10 METABOLITE) (QUINIDINE) BIRD FANCIERS DISEASE Refer to QUINIDINE Refer to AVIAN PRECIPITINS BISMUTH RANDOM URINE Urine UNINSURED LL Min volume: 20ml Store and ship refrigerated Ensure hands are washed and clothes are free of contamination. For industrial exposure collect specimen at the end of the work shift. A random urine test includes creatinine to be performed by the referred out testing site TAT 5-10 days BLASTOMYCES ANTIBODY 9037 Do not centrifuge tube PLAIN RED N/C PHL (BLASTOMYCOSIS ANTIBODY DERMATITIDIS) REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM TAT 30 days BLASTOMYCOSIS 9038 Culture N/C PHL CULTURE DERMATITIDIS Skin scraping REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM TAT 30 days BLEEDING TIME, DUKE METHOD BLEEDING TIME, IVY METHOD BLOOD CULTURE BLOOD FILM EXAMINATION NO LONGER AVAILABLE Refer to CULTURE & SENSITIVITY - BLOOD Refer to COMPLETE BLOOD COUNT BLOOD GROUP C171 Collect blood in a PINK top tube (K2EDTA). PINK TOP OHIP LL (ABO, Rh(D) (ABO & TYPE) Collect a full tube. Mix thoroughly by gentle (Group RH) Inversion. Send entire rube. Store and ship Refrigerated at 2-8 o C. ADDITIONAL INFORMATION: DO NOT CODE test code C171 if Prenatal Screen requested along with Blood Group. CODE test code C176 which includes Blood Group and RH. DO NOT SEPARATE TAT 2 days BLOOD GROUP ANTIBODY IDENTIFICATION Refer to ANTIBODY IDENTIFICATION TEST SPECIFICATION GUIDE - SECTION B Page 4 of 6 CML HealthCare Inc Test Specification Guide Version Oct-2015

31 BLOOD GROUP AND C172 Collect blood in PINK top tube (K2EDTA). PINK TOP OHIP LL RH PHENOTYPE Collect a full tube. Mix thoroughly by gentle (ABO, Rh(D), (GENOTYPE) inversion. Send entire tube. Store and ship refrigerated at 2-8 o C. ADDITIONAL INFORMATION: Test code C172 includes Blood Group. DO NOT CODE test code C171 separately. DO NOT SEPARATE TAT 2 days BLOOD GROUP PRENATAL Ab (ABO & Ab SCREEN PRENATAL SCREEN TYPE & SCREEN) BLOOD GROUP ANTIGENS - Eg Kell, Duffy, KIDD Refer to BLOOD GROUP and Refer to ANTIBODY SCREEN NO LONGER AVAILABLE BLOOD, QUALITATIVE Urine OHIP CML 10 ml random urine Submit in a yellow cap conical tube BLOOD PRESSURE 995 Performed at limited sites UNINSURED CML MONITORING TAT 4 days BLOOD TYPE (ABO, Rh(D), (ABO & TYPE) (BLOOD GROUP & Rh(D) (Rh TYPE) BNP (NT-PRO) BORDETELLA PERTUSSIS ANTIBODY (WHOOPING COUGH) Refer to BLOOD GROUP Refer to B-TYPE NATRIURETIC PEPTIDE SERUM TESTING NO LONGER AVAILABLE BORDETELLA PERTUSSIS 9047 Swab State source N/C PHL (WHOOPING COUGH) Use the PHL Kit REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM TAT 4 days TEST SPECIFICATION GUIDE - SECTION B Page 5 of 6 CML HealthCare Inc Test Specification Guide Version Oct-2015

32 BORRELIA BURGDORFERI 9045 Do not centrifuge tube PLAIN RED N/C PHL ANTIBODY Patient s history and symptoms are mandatory (LYME DISEASE) REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM TAT 15 days BROAD SPECTRUM DRUG SCREEN BROMIDE Refer to DRUG SCREEN BROAD SPECTRUM NO LONGER AVAILABLE BRUCELLA ANTIBODY 9007 Do not centrifuge tube PLAIN RED N/C PHL REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM Testing Includes Brucella Abortus and Brucella Melitensis TAT 5 days BUGS (ARTHROPODS) (LICE) Refer to ARTHROPOD IDENTIFICATION BUN (UREA) Refer to UREA BUTABARBITAL 9471 Urine OHIP HLRC 25 ml random urine Submit in a 90 ml orange cap container BUTAZOLIDINE (PHENYLBUTAZONE) NO LONGER AVAILABLE TEST SPECIFICATION GUIDE - SECTION B Page 6 of 6 CML HealthCare Inc Test Specification Guide Version Oct-2015

33 C1 ESTERASE INHIBITOR (COMPLEMENT C1) C1 ESTERASE INHIBITOR, FUNCTIONAL Refer to COMPLEMENT C1 ESTERASE INHIBITOR Refer to COMPLEMENT C1 ESTERASE INHIBITIOR, FUNCTIONAL C1Q IMMUNE COMPLEXES 688 Serum GOLD SST OHIP HLRC (C1Q COMPLEMENT BINDING ACTIVITY) Minimum Volume required: 1 ml (C1Q IMMUNE COMPLEXES) Only performed if CH50 is low (COMPLEMENT C1Q) Separate and freeze within 1-hour of clotting FREEZE SERUM AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines. TAT 25 days C2 (COMPLEMENT C2) C3 (COMPLEMENT C3) C4 (COMPLEMENT C4) C5 (COMPLEMENT C5) C6 (COMPLEMENT C6) CD3, CD4, CD8 (LYMPHOCYTE MARKER-T CELL ONLY) (T CELL LYMPHOCYTE MARKER ONLY) Refer to COMPLEMENT C2 Refer to COMPLEMENT C3 Refer to COMPLEMENT C4 Refer to COMPLEMENT C5 Refer to COMPLEMENT C6 Refer to LYMPHOCYTE MARKER T CELLS ONLY C PEPTIDE 470 Serum PLAIN RED OHIP LL (CONNECTING PEPTIDE) Minimum Volume required: 1.0 ml Collect blood in a PLAIN RED top tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Transfer an aliquot of serum to a labelled tube, cap tightly and FREEZE at -20 o C Do not use SST tubes. Store and ship frozen at -20 o C TAT 5 days C REACTIVE PROTEIN (CRP) (C REACTIVE PROTEIN) SEE C-REACTIVE PROTEIN HIGH SENSITIVITY C REACTIVE PROTEIN 665HS Serum GOLD SST OHIP CML HIGH SENSIVITY (CRP HIGH SENSIVITY) C TELOPEPTIDE 9164 Serum GOLD SST UNINSURED HLRC Minimum volume required: 1 ml Fasting specimen preferred FREEZE SERUM AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines. TAT 20 days TEST SPECIFICATION GUIDE - SECTION C Page 1 of 30 CML HealthCare Inc Test Specification Guide Version: Oct-2015

34 CA Serum GOLD SST UNINSURED CML (OV 125) (CANCER ANTIGEN 125) Should not to be used as a diagnostic screening test. TAT 5 days CA 15 3, Breast 3011 Serum GOLD SST UNINSURED LL (CANCER ANTIGEN 15-3) Minimum Volume required: 1.0 ml (CARBOHYDRATE ANTIGEN 15-3) Collect blood in SST tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Transfer an aliquot of serum to a labelled tube, cap tightly and FREEZE at -20 o C. Store and ship frozen at -20 o C TAT 1 week CA 19 9, Pancreas 3012 Serum GOLD SST UNINSURED LL (CANCER ANTIGEN 19-9) Minimum Volume required: 1.0 ml (CARBOHYDRATE ANTIGEN 19-9) Collect blood in SST tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Store and ship refrigerated at 2-8 o C TAT 1 week CADMIUM, BLOOD 9680 Blood ROYAL BLUE UNINSURED HLRC Minimum Volume required: 4 ml K2 EDTA Do not open tube TAT 21 days CADMIUM, RANDOM URINE 9680R Urine UNINSURED HLRC 15 ml aliquot random urine Submit in a white cap 90 ml container TAT 21 days CADMIUM, 24-HOUR URINE 9680U 24 Hour Urine UNINSURED HLRC 15 ml aliquot submit in a white cap 90 ml container State total 24-hour volume on the OHIP Requisition, on the specimen container and in Notes & Instructions. Retain a duplicate 50 ml sample in the fridge until test is reported. CAFFEINE 9129 Serum PLAIN RED UNINSURED HLRC (CAFFEINE- QUANTITATIVE) Minimum Volume required: 1 ml Collect hours after last dose Record time in hours that have elapsed between last dose and specimen collection. TEST SPECIFICATION GUIDE - SECTION C Page 2 of 30 CML HealthCare Inc Test Specification Guide Version: Oct-2015

35 CALCIDIOL (UNINSURED) 9802 Serum GOLD SST UNINSURED CML (25 HYDROXY VITAMIN D) Minimum volume required: 2 ml (VITAMIN D) Centrifuge SST Store and ship refrigerated No pour-off required TAT 2 days CALCIDIOL (INSURED) 606 Serum GOLD SST OHIP CML (25 HYDROXY VITAMIN D) (VITAMIN D) Minimum volume required: 2 ml Centrifuge SST Store and ship refrigerated No pour-off required Patient must meet eligibility criteria for insurable Calcidiol testing TAT 2 days CALCITONIN 301 Serum GOLD SST OHIP DYN Minimum Volume required: 3 ml Fasting sample required. Centrifuge, separate, freeze within 30-minutes of clotting. FREEZE SERUM AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines. TAT 14 days CALCITRIOL 528 Serum GOLD SST OHIP LL (VITAMIN D 1-25) Minimum volume required: 2.0 ml (1, 25 DIHYDROXY VITAMIN D) Collect blood in SST tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Serum must NOT be aliquoted, the testing bench must receive the specimen in the primary SST tube. Store and ship refrigerated at 2-8 o C. TAT 1 week CALCIUM 045 Serum GOLD SST OHIP CML CALCIUM, CORRECTED 045C Serum GOLD SST OHIP CML Testing includes serum calcium and albumin. State test in Notes & Instructions and on the OHIP requisition. TEST SPECIFICATION GUIDE - SECTION C Page 3 of 30 CML HealthCare Inc Test Specification Guide Version: Oct-2015