Lab 02: Blood Cytology (20 points)
|
|
- Maude Hutchinson
- 8 years ago
- Views:
Transcription
1 Pierce College Putman/Biol 242 Name: Lab 02: Blood Cytology (20 points) Reference: Marieb & Mitchell 9 th Ed: 29A (Activities 1, 2, 3, 4, 7); 10 th Ed: Exercise 29 (Activities 1, 2, 3, 4, 7). Pierce College Student Outcomes: Lab Outcome 1: Correlate qualitative and quantitative information about the constituents of blood via microscopic examination. Lab Outcome 2: Generate a hematology slip following the performance of a hematocrit and a differential white blood cell count from a blood smear stained with Wright s stain. Lab Outcome 3: Recognize the ABO blood type and Rh factor from an agglutination test and describe the frequencies of blood types among the various ethnic populations applying genetic concepts. Objectives for Lab Exam: 1. State the ph, color, degree of transparency and consistency of plasma. 2. Explain the protocol of safely obtaining blood for observation under a microscope. 3. Explain the necessary protocols when working with human blood. 4. Explain how to correctly make a blood smear. 5. Explain how to correctly make a Wright s stain of a blood smear. 6. Give the components of Wright s stain and state what they stain, what they do not stain, and how this allows us to identify the various leucocytes. 7. Identify erythrocytes, neutrophils, eosinophils, basophils, lymphocytes, monocytes, platelets. 8. List the leukocytes in order from most to least common, and state their functions. 9. Describe hematocrit protocol. 10. State what normal hematocrit levels are. 11. Explain the theory behind ABO and Rh blood typing. 12. State why it s important to know blood types before blood transfusions are carried out. *Make sure you obtain the microscope you initially calibrated so you don t have to recalibrate your scope! Activity 1: Determining the Physical Characteristics of Plasma 1. From your textbook, answer the following questions about plasma (blood): a. ph range of plasma: b. Color: Putman/Biol 242 Lab 2/ /Page 1
2 c. Degree of transparency: d. Viscosity compared to water: Activity 2: Performing a Blood Smear and Wright s Stain. Lab safety advisory: Biohazard! Human blood is a BIOHAZARD and must be worked w with using strict protocols. Anything that has come in contact with human blood is a BIOHAZARD. At Pierce College, we use animal (dog) blood from our or another veterinary clinic. You are still expected to follow human protocols for this blood. About the Wright s Stain Wright s stain is made of eosin and hematoxyln. The granules of granulocytes (eosinophins, lymphocytes and basophils) are stained by Wright s stain. Eosinophils pick up eosin strongly, so their large granules stain red. Neutrophils pick up eosin weakly, so their small granules stain faint pink. Basophils do not pick up eosin; instead, they pick up hematoxyln, which stains their granules blue. Hematoxyln also stains the nuclei of all of the leucocytes, allowing us to differentiate nuclear morphology. The agranulocytes, lymphocytes and monocytes, pick up neither eosin nor hematoxyln, so their granules don t stain in Wright s stain, but their nuclei are stained by hematoxyln. Making a Blood Smear Step 1: Move unneeded materials off your lab bench. Step 2: Disinfect your lab bench with the provided sodium hypochlorite (bleach) solution and a paper towel. Step 3: Wash your hands, put on gloves. Step 4: At your work station, lay down a paper towel. Obtain two microscope slides. Step 5: If needed, gently rock the blood sample provided; do not shake the blood as this will damage the blood cells. Step 6: Dip the end of a pipette into the blood sample to obtain a droplet of blood. Step 7: Apply the droplet to the end of a microscope slide; with the second microscope slide, immediately draw the blood over the slide so that it covers a maximum area. To do this, place the edge of the second slide so that it touches the drop of blood; then draw the second slide across the first slide steadily, with one motion, without starting/stopping, so that the blood is PULLED across the first slide. Do this once only; do NOT keep trying to spread the blood because this will cause the blood to clump; the blood smear MUST be even and thin! Putman/Biol 242 Lab 2/ /Page 2
3 Step 8: Allow the blood on the slide to air dry. This should take only a few minutes Wright s Stain Protocol Step 1: Once the blood is air dried, put the slide on a staining tray over a sink and saturated with Wright s stain. Stain for 1 minute. Step 2: After 1 minute, dip the slide, down and up, into distilled water rinse 1, then down and up into distilled water rinse 2. Step 3: Wipe the back of the wet slide with a paper towel and keep the slide in a vertical position on a paper towel until it is dry. Step 4: Don t put a cover slip on this slide as this is a temporary slide. Step 5: After the slide is dry, examine the cells under the microscope. Make sure you begin with lowest power, 40x or 32x, and work your way up to 400x. After you have focused on your cells at 400x, apply a drop or two of immersion oil, then slide the 100x objective (1000x total magnification) into the oil and observe. Step 6: Locate a white blood cell and show your instructor. Have your instructor sign below so you receive credit for your properly-made Wright s stain: Instructor s signature Step 7: After your instructor has checked your work and signed above, place the slide in the indicated biohazard container (red box). Step 8: If you are finished, triple-clean the 100x lens. If you have time to continue on to the next section, you may wait to clean the 100x lens. Activity 3: Identifying Leucocytes 1. Using a commercial slide of human blood, identify, draw and label erythrocytes, thrombocytes (platelets), neutrophils, lymphocytes, monocytes, eosinophils and basophils. Be sure to include a size rule and have your instructor sign your drawings for credit. If you make all of your drawings to the same scale, one size rule will suffice. You may include your drawings in the space provided on page 4. Use 1000x total magnification with immersion oil; be sure to triple clean both the microscope slide and 100x objective when finished! Putman/Biol 242 Lab 2/ /Page 3
4 Do drawings of blood cells on this page! Putman/Biol 242 Lab 2/ /Page 4
5 2. Answer the following questions: 1. List the leukocytes in order from most to least common. 2. Which two leucocytes does the eosin in Wrights stain stain? 3. Regarding granule size the how strongly eosin is taken up by those granules, how can you use eosin to differentiate between the two leucocytes it stains? 4. How can eosin be used to identify monocytes and lymphocytes? 5. How can hematoxyln be used to identify basophils? 6. How can hematoxyln be used to differentiate between neutrophils, lymphocytes, monocytes, eosinophils and basophils? 7. What is the function of neutrophils? 8. What is the function of eosinophils? 9. What is the function of basophils? 10. What is the function of lymphocytes? 11. What is the function of monocytes? Putman/Biol 242 Lab 2/ /Page 5
6 Activity 4: Conducting a Differential WBC Count Differential counts are used in clinics to determine if certain blood cell types are higher or lower than normal, thereby providing valuable clues as to the health of a patient. Step 1: Using a commercially-prepared blood slide, perform a differential WBC count, identifying at least 50 leucocytes. Work with your lab partner. Start with a corner of the slide and work your way up, over, down, over, up, etc. until you have identified at least 25 leucocytes; have your lab partner keep a tally for you in the table below. After you have identified at least 25 cells, switch roles and record data for your lab partner as she or he identifies at least 25 leucocytes. WBC Tally Number % Normal % Neutrophil Explanation Lymphocyte Monocyte Eosinophil Basophil Total: Step 2: Combine data with your lab partner. Add up the number of each leucocyte counted, then add up the total of all leucocytes counted. Calculate the percent of each leucocyte. Finally, look up the normal % for each leucocyte in reference materials given to you and add it to the table. Step 3: Compare the percentages of each type of WBC. Are all of the counts within normal range? If any are high or low, explain what might be going. If values are within a normal range, simply write within normal range. Activity 5: Determining the Hematocrit (PCT) Hematocrit (packed-cell count or PCT) allows us to determine what percentage of a blood sample is composed of blood cells, thus giving us information on the possible diagnosis of various blood pathologies, including polycythemia. Step 1: Put gloves on. Obtain a heparinized capillary tube. Heparin is an agent that inhibits blood from coagulating within the capillary tube. Putman/Biol 242 Lab 2/ /Page 6
7 Step 2: Pack the capillary tube with blood until it is at least ½ full by dipping it up and down in the blood sample. When finished, press tube down into Cryoseal clay and turn to seal. Step 3: With a small piece of wet paper towel, wipe the excess blood from the outside of the capillary tube. Step 4: Place capillary tube in centrifuge with clay plug facing outwards so blood remains in tube when centrifuge is turned on. Note the number of the slot in which you placed your tube. Also make sure it s balanced with a tube on the other side; your instructor will help you with this. Step 5: When everyone has loaded their tube into the centrifuge, your instructor will turn the centrifuge on for 5 minutes. This will spin the formed elements down, erythrocytes first, a very thin leucocyte line on top of the erythrocytes, the remainder being plasma. Step 6: When ready, pick up your centrifuge tube. Obtain a millimeter ruler. Measure the total height of the column of blood to the nearest 10 th of a millimeter. Also measure the height of the erythrocytes to the nearest 10 th of a millimeter. Be careful to begin your measurement at the bottom of the column of blood, not including the clay plug. Record your data below and determine the hematocrit, which is the %RBC in your sample. Place capillary tube in red biohazard box when finished. Total Height of Blood in Capillary Tube, mm Total Height of RBCs in Capillary Tube, mm Hematocrit, %RBCs Answer the Following Questions: 1. What is a normal hematocrit value? 2. If the hematocrit you determined is different than a clinically normal hematocrit value, propose an explanation! Otherwise, write hematocrit normal. Putman/Biol 242 Lab 2/ /Page 7
8 Activity 6: Typing for ABO and Rh Blood Groups. All cells contain antigens in their glycocalyx. The immune system reacts to non-self antigens by producing antibodies which attach to the non-self antigens. This causes cells involved in the immunological response to attack the non-self antigens and to cause agglutination or clumping together of the non-self antigens. If the non-self antigens are located on erythrocytes introduced into the body during a blood transfusion, serious clinical consequences, including death, may result as the blood agglutinates and blocks arterioles and venules. Individuals with type A, B or AB have A, B or AB on their erythrocytes; people who are type O have none of these antigens. During a transfusion, if someone who is type A receives any blood containing B antigen, as from type A or type AB, this will cause an antigen-antibody reaction and agglutination. Similarly, if an individual who is type B receives any blood containing A antigen, as from type B or AB, agglutination will result. Type AB can receive A, B, AB or O without danger of agglutination. Type O can only receive type O as type A or B antigens will elicit agglutination. The Rh blood group, or rhesus factor, works in a manner similar to the ABO blood group. An individual who is + for the rhesus factor carries the antigen on his/her erythrocytes; - individuals do not carry the rhesus factor. An Rh + individual can receive Rh + blood without fear of agglutination. If an Rh individual receives Rh + blood, then antibodies are produced and agglutination will occur. Typing for ABO and Rh Step 1: Obtain an ABO/Rh test kit. The kit will consist of blood serum from a patient, three antibody solutions (anti-a, anti-b and anti-rh), a plastic typing tray and plastic toothpicks. Write the patient s name down below (only type one of the patients). Patient s Name: Blood Type: Step 2: Place two drops of serum into each of the three wells. Place two drops of each of the antisera into each of the corresponding wells and stir each time with a clean plastic toothpick. Rinse the toothpicks when you re done and put them back into their holders (don t throw away). Step 3: After five minutes, place the plastic tray on some print such as the print of this page. If you can read through the serum/antibody combination, the test is negative for that antigen. If you can t read through the serum/antibody combination, that s a positive reaction. If you didn t get a positive reaction, examine positive reactions obtained by other students so that you learn what a positive reaction looks like. Putman/Biol 242 Lab 2/ /Page 8
9 Answer the following question: 1. Why is blood typing so important before surgical procedures are attempted! ~When you re finished, help clean up! 1. Is your lab bench clean and wiped down with antiseptic solution? 2. Are all materials returned to their proper place? 3. Is there any trace of oil on your microscope lenses OR microscope slides? TRIPLE CLEAN!!! 4. Is all refuse disposed of properly? 5. Is the lab generally in order? Putman/Biol 242 Lab 2/ /Page 9
Exercise 9: Blood. Readings: Silverthorn 5 th ed, 547 558, 804 805; 6 th ed, 545 557, 825 826.
Exercise 9: Blood Readings: Silverthorn 5 th ed, 547 558, 804 805; 6 th ed, 545 557, 825 826. Blood Typing The membranes of human red blood cells (RBCs) contain a variety of cell surface proteins called
More informationABO-Rh Blood Typing Using Neo/BLOOD
ABO-Rh Blood Typing Using Neo/BLOOD Objectives Determine the ABO and Rh blood type of unknown simulated blood samples. Prepare a simulated blood smear. Examine a prepared blood smear under the microscope
More informationABO-Rh Blood Typing With Synthetic Blood
70-0101 ABO-Rh Blood Typing With Synthetic Blood Teacher s Manual World-Class Support for Science & Math The ability to type blood is an invaluable tool in the fields of medicine and criminology. Using
More informationserology Agglutination Techniques and Blood Cell Identification
Serology: Agglutination Techniques and Blood Cell Identification S erology is a branch of immunology dealing with techniques to identify and measure antigens, and to detect serum antibodies. Agglutination
More information06-6542 n/a MYSTERY OF THE BLOOD STAIN (RE)
DATA SHEET Order code Manufacturer code Description 06-6542 n/a MYSTERY OF THE BLOOD STAIN (RE) The enclosed information is believed to be correct, Information may change without notice due to product
More informationPreparation of Blood Films
Preparation of Blood Films Principle: Blood film enables us to evaluate WBC, RBC, and PLT morphology, also, allows us to perform differential WBC count, furthermore estimation of WBC and platelets counts
More informationLAB: Blood Exploration
Name: Period: Date: LAB: Blood Exploration Introduction A liquid called plasma makes up about half (55%) of the content of blood. Plasma contains proteins that help blood to clot, transport substances
More informationWORKING SAFELY WITH BLOOD:
Biology 212: Human Anatomy and Physiology II *************************************************************************************************** BLOOD ***************************************************************************************************
More informationLaboratory 12 Blood Cells
Laboratory 12 Blood Cells Objectives: Identify microscopically each of the following: erythrocytes (red blood cells or RBCs), the five types of leukocytes (white blood cells or WBCs), and thrombocytes
More informationBlood Sticky, opaque fluid with a metallic taste (Fe 2+ ) Varies from scarlet (P O2 = 100) to dark red (P O2 = 40) ph is between 7.35 and 7.45 Average volume in an adult is 5 L (7% of body weight) 2 L
More informationBlood Typing Laboratory Exercise 40
Blood Typing Laboratory Exercise 40 Background Blood typing involves identifying protein substances called antigens that are present in red blood cell membranes. Although there are many different antigens
More informationKEY CHAPTER 14: BLOOD OBJECTIVES. 1. Describe blood according to its tissue type and major functions.
KEY CHAPTER 14: BLOOD OBJECTIVES 1. Describe blood according to its tissue type and major functions. TISSUE TYPE? MAJOR FUNCTIONS connective Transport Maintenance of body temperature 2. Define the term
More informationCan receive blood from: * I A I A and I A i o Type A Yes No A or AB A or O I B I B and I B i o Type B No Yes B or AB B or O
Genetics of the ABO Blood Groups written by J. D. Hendrix Learning Objectives Upon completing the exercise, each student should be able: to explain the concept of blood group antigens; to list the genotypes
More informationCOMPARING PLANT AND ANIMAL CELLS
COMPARING PLANT AND ANIMAL CELLS OBJECTIVES: Distinguish between plant and animals cells by their structures Demonstrate the benefit of stains Acquire ability to prepare wet mounts SAFETY: Methylene blue
More informationSTAINING OF PBF AND INTERPRETATION OF NORMAL AND ABNORMAL RED CELL MORPHOLOGY
9 STAINING OF PBF AND INTERPRETATION OF NORMAL AND ABNORMAL RED CELL MORPHOLOGY 9.1 INTRODUCTION A peripheral blood smear (peripheral blood film) is a glass microscope slide coated on one side with a thin
More informationThibodeau: Anatomy and Physiology, 5/e. Chapter 17: Blood
Thibodeau: Anatomy and Physiology, 5/e Chapter 17: Blood This chapter begins a new unit. In this unit, the first four chapters deal with transportation one of the body's vital functions. It is important
More informationGENETICS OF HUMAN BLOOD TYPE
GENETICS OF HUMAN BLOOD TYPE Introduction The genetics of blood types is relatively simple when considering any one blood protein. However, the complexity increases when one considers all the different
More informationGranulocytes vs. Agranulocytes
Leukocytes are white blood cells (AKA colorless (non-pigmented) blood cells). (Much) smaller in number than RBCs. Unlike RBCs, there are several different types of WBCs. All contain a visible nucleus.
More informationBlood Stains at the Crime Scene Forensic Investigation
Blood Stains at the Crime Scene Forensic Investigation Introduction Blood stains at a crime scene can be crucial in solving the crime. Numerous analytical techniques can be used to study blood stains.
More informationCardiovascular System. Blood Components
Cardiovascular System Blood Components 1 Components of Blood Formed elements: erythrocytes, leukocytes, platelets Plasma: water, proteins, other solutes The components of blood can be divided into two
More informationHiPer Blood Grouping Teaching Kit
HiPer Blood Grouping Teaching Kit Product Code: HTI008 Number of experiments that can be performed: 100 Duration of Experiment: Protocol: 15 minutes Storage Instructions: The kit is stable for 6 months
More informationBlood. Functions of Blood. Components of Blood. Transporting. Distributing body heat. A type of connective tissue. Formed elements.
Blood Functions of Blood Transporting nutrients respiratory gases waste products Distributing body heat Components of Blood A type of connective tissue Formed elements Living blood cells Plasma Nonliving
More informationA&P 2 Blood Lab Guide In-Lab Exercises
A&P 2 Blood Lab Guide In-Lab Exercises Have someone in your group read the following out loud, while the others read along: In this "Lab Guide", we will be looking at the blood and some common blood tests.
More informationLABORATORY 2 Staining and processing of blood parasites Differential counts of leukocytes (giemsa stains)
LABORATORY 2 Staining and processing of blood parasites Differential counts of leukocytes (giemsa stains) SPECIMENS TO BE STAINED 1. Thin and thick blood smears from a patient returning from Africa - case
More informationBlood Type Testing Lab Report Section 1101 Nattanit Trakullapphan (Nam) Chawalnrath Wongdeshanan (Kat)
Blood Type Testing Lab Report Section 1101 Nattanit Trakullapphan (Nam) Chawalnrath Wongdeshanan (Kat) Introduction: figure 1.1 (Blood type n.d.) figure 1.2 (Blood type, Antigens-Antibodies n.d.) Multiple
More informationHUMAN BLOOD TYPE: TESTING FOR ABO AND Rh FACTORS STANDARDS 3.3.7.B, 3.3.7.C 3.3.10.B, 3.3.10.C
HUMN LOOD TYPE: TESTING FOR O ND FCTORS STNDRDS 3.3.7., 3.3.7.C 3.3.10., 3.3.10.C Westminster College INTRODUCTION lood is a living tissue which circulates through the human body providing oxygen and nutrients
More informationEssentials of Anatomy and Physiology, 9e (Marieb) Chapter 10 Blood. Short Answer. Figure 10.1
Essentials of Anatomy and Physiology, 9e (Marieb) Chapter 10 Blood Short Answer Figure 10.1 Using Figure 10.1, identify the following: 1) The neutrophil is indicated by letter. Answer: A Diff: 1 Page Ref:
More informationDirect Antiglobulin Test (DAT)
Exercise 8 Exercise 9 Direct Antiglobulin Test (DAT) Elution Study Task Aim Introduction To perform the DAT and elution procedure with correct interpretation of results. To perform with 100% accuracy the
More informationCORD BLOOD EVALUATION
CORD BLOOD EVALUATION Principle: When there is incompatibility between a mother s antibodies and an infant s red blood cell antigens, the infant is at risk of developing Hemolytic Disease of the Fetus
More informationExercise 2. The Compound Light Microscope
6 Exercise 2 The Compound Light Microscope INTRODUCTION: Student Learning Objectives: After completing this exercise students will: a. Demonstrate proficient use of the microscope using low, high dry,
More informationPre-Lab Questions. 1. What is cell theory? 2. What do all cells contain? 3. What is a prokaryote? 4. What is a eukaryote? 5. What is an organelle?
Name: TOC# Background Ever since the first microscope was used, biologists have been interested in studying the cellular organization of all living things. After hundred s of years of observations by many
More informationSTEP-BY-STEP INSTRUCTIONS FOR INVESTIGATIONAL USE. Rapid HCV Antibody Test FOR ORAQUICK RAPID HCV ANTIBODY TEST
Before performing testing, all operators MUST read and become familiar with Universal Precautions for Prevention of Transmission of Human Immunodeficiency Virus, Hepatitis B Virus, and other Blood-borne
More informationBlood-Based Cancer Diagnostics
The Biotechnology Education Company Blood-Based Cancer Diagnostics EDVO-Kit 141 Store entire experiment at room temperature. EXPERIMENT OBJECTIVE: The objective of this experiment is to learn and understand
More information1. BLOOD GROUP SYSTEMS. Page 1. Haematology LECTURE 10. BLOOD GROUPS AND TRANSFUSIONS OVERVIEW. 1. Blood Group Systems
Undergraduate Course in Veterinary Clinical PathologySocrates Programme Haematology LECTURE 10. BLOOD GROUPS AND TRANSFUSIONS 10-1 OVERVIEW 1. Blood Group Systems 2. Blood group testing and cross-matching
More informationUrinalysis and Body Fluids CRg
Urinalysis and Body Fluids CRg Unit 2; Session 1 Urine Microscopic Examination The Complete Urinalysis Physical properties already covered Chemical analysis in the next unit Microscopic our current focus
More informationGIEMSA STAIN PREANALYTICAL CONSIDERATIONS
GIEMSA STAIN PREANALYTICAL CONSIDERATIONS I. Principle Giemsa stain is used to differentiate nuclear and/or cytoplasmic morphology of platelets, RBCs, WBCs, and parasites (1,2). The most dependable stain
More informationExplain the role of blood and bloodstain patterns in forensics science. Analyze and identify bloodstain patterns by performing bloodstain analysis
Lab 4 Blood Learning Objectives Explain the role of blood and bloodstain patterns in forensics science Analyze and identify bloodstain patterns by performing bloodstain analysis Introduction Blood, a
More informationHematology Sequence. Blood Labs ORIENTATION OBJECTIVES. c. Microscopic Exam of a Peripheral Blood Smear (several parts)
Hematology Sequence Blood Labs ORIENTATION OBJECTIVES Objectives Procedure 1. Read the three sections that follow: a. Hematocrit b. Preparing a peripheral lood smear c. Microscopic Exam of a Peripheral
More informationHaematology what does your blood test mean?
Haematology what does your blood test mean? This document provides some reading material to be used in preparation for the Haematology what does your blood test mean? workshop at RMIT s Experience Health
More informationChapter 18. Blood Types
Chapter 18 Blood Types Blood Types blood types and transfusion compatibility are a matter of interactions between plasma proteins and erythrocytes Karl Landsteiner discovered blood types A, B and O in
More informationIntroduction. Laboratory Procedure Manual
Exercise 9 Elution Study Objectives: 1. State when an elution procedure may be performed. 2. List four situations in which the performance of an elution may provide helpful information. 3. List three types
More informationEDUCATIONAL COMMENTARY RED BLOOD CELLS AND WHITE BLOOD CELLS IN URINALYSIS
URINALYSIS Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain FREE CME/CMLE credits click on Earn CE Credits under Continuing
More informationBlood Physiology. Practical 4. Contents. Practical tasks. Erythrocytes The blood types
Blood Physiology Practical 4 Contents Erythrocytes The blood types Practical tasks Determination of blood groups of the ABO system Determination of the Rhesus system (Rh factor) The cross matching test
More informationRhD typing. Practice for IV year medical students. Zita Csernus MD. National Blood Transfusion Service Blood Transfusion Centre Pécs
immunisation Bed side test Antibody tests RhD typing Practice for IV year medical students Zita Csernus MD National Blood Transfusion Service Blood Transfusion Centre Pécs Rh Blood Group System Discovery:
More informationJOINT COMMISSION INTERNATIONAL ACCREDITATION STANDARDS FOR. 2nd Edition
JOINT COMMISSION INTERNATIONAL ACCREDITATION STANDARDS FOR CliniCAl laboratories 2nd Edition Effective 1 April 2010 International Patient Safety Goals (IPSG) Goals The following is a list of all goals.
More informationCHAPTER 14: CARDIOVASCULAR SYSTEM: BLOOD. 1. Describe blood according to its tissue type and major functions.
OBJECTIVES: 1. Describe blood according to its tissue type and major functions. 2. Define the term hematology. 3. Name the average volume of blood in a human. 4. Name the two major components of blood
More informationHISTOLOGY LABORATORY. Microscope Orientation and Blood Smear Lab
HISTOLOGY LABORATORY Microscope Orientation and Blood Smear Lab For practicing how to use the microscope DO NOT use the blood smear slide (it is too boring for the lower mags). Use a slide from the white
More informationINDICATIONS FOR BLOOD PRODUCT TRANSFUSIONS
INDICATIONS FOR BLOOD PRODUCT TRANSFUSIONS Sarah Perry, LVT, BS, VTS (ECC) Licensed Veterinary Technician Animal Neurology, Rehabilitation and Emergency Center 1120 Welch Rd. Commerce, MI 48390 A very
More informationWhite Blood Cells (WBCs) or Leukocytes
Lec.5 Z.H.Al-Zubaydi Medical Physiology White Blood Cells (WBCs) or Leukocytes Although leukocytes are far less numerous than red blood cells, they are important to body defense against disease. On average,
More informationAppendix H IBC Managing Biohazardous Waste SOP
Biohazardous waste is managed under the State of Rhode Island s Regulated Medical Waste Regulations (Regulation DEM-OWM-MW-1-2009, amended July, 2010). http://www.dem.ri.gov/pubs/regs/regs/waste/medwaste10.pdf
More informationBLOOD-Chp. Chp.. 6 What are the functions of blood? What is the composition of blood? 3 major types of plasma proteins
6.1 Blood: An overview BLOOD-Chp Chp.. 6 What are the functions of blood? Transportation: oxygen, nutrients, wastes, carbon dioxide and hormones Defense: against invasion by pathogens Regulatory functions:
More information3. The Circulatory System
3. The Circulatory System A. Introduction B. Blood 1. Circulatory system transports water, electrolytes, hormones, enzymes, antibodies, cell, gases and nutrients to all cells and carries away metabolic
More informationCollect and label sample according to standard protocols. Gently invert tube 8-10 times immediately after draw. DO NOT SHAKE. Do not centrifuge.
Complete Blood Count CPT Code: CBC with Differential: 85025 CBC without Differential: 85027 Order Code: CBC with Differential: C915 Includes: White blood cell, Red blood cell, Hematocrit, Hemoglobin, MCV,
More informationBlood. Blood. Blood Composition. Blood Composition. Fractionation & Hemopoesis
Blood Blood Chapter 19 Blood is a specialized fluid connective tissue that contains cells suspended in a fluid matrix Functions of blood include: Transport of dissolved gases, nutrients, hormones and metabolic
More informationWhat Forensics Information Does Blood Typing Provide?
The Biotechnology Education Company EDVO-Kit 191 What Forensics Information Does Blood Typing Provide? See Page 3 for storage instructions. EXPERIMENT OBJECTIVE: The objective of this experiment is to
More informationBlood Collection and Processing SOP
Brisbane Breast Bank Blood Collection and Processing SOP Breast Pathology Laboratory University of Queensland Centre for Clinical Research Blood Collection We collect 30ml of blood from patients who have
More informationPhlebotomy Handbook Blood Collection Essentials Seventh Edition
Phlebotomy Handbook Blood Collection Essentials Seventh Edition Diana Garza Kathleen Becan-McBride Chapter Four The Cardiovascular System Introduction Circulatory system is a transport system. Contributes
More informationSafe Blood and Blood Products
Safe Blood and Blood Products Module 3 Blood Group Serology Safe Blood and Blood Products Module 3 Blood Group Serology Conversion of electronic files for the website edition was supported by Cooperative
More informationAppendix H Managing Biohazardous Waste SOP
Biohazardous waste is managed under the State of Rhode Island s Regulated Medical Waste Regulations (Regulation DEM-OWM-MW-1-2009, amended July, 2010). http://www.dem.ri.gov/pubs/regs/regs/waste/medwaste10.pdf
More informationPlease list four delivery functions of blood, two regulatory functions, and two protection functions. Delivery (distribution) functions
Chapter 17 Blood Please list four delivery functions of blood, two regulatory functions, and two protection functions. Delivery (distribution) functions Names Period 1-3 - 2-4 - Regulatory functions 1-2
More informationA simple differential stain of blood smears using black Quink
A simple differential stain of blood smears using black Quink Chris Thomas, 3 Hall End, Milton, Cambridge CB24 6AQ, chris@miltoncontact.com Published in: Micscape http://www.microscopy-uk.org.uk/mag/artsep15/ct-quink-blood-stain.pdf
More informationBLOOD FILM STAINING EFFECTS
An Educational Supplement prepared by ALQEP May 2004 Introduction The stained peripheral blood film is one of the world s most widely and frequently used tests. Since its introduction in the late nineteenth
More informationDEPARTMENT OF CLINICAL LABORATORY SCIENCES SCHOOL OF HEALTH TECHNOLOGY AND MANAGEMENT THE UNIVERSITY AT STONY BROOK STONY BROOK, NEW YORK 11794-8205
DEPARTMENT OF CLINICAL LABORATORY SCIENCES SCHOOL OF HEALTH TECHNOLOGY AND MANAGEMENT THE UNIVERSITY AT STONY BROOK STONY BROOK, NEW YORK 11794-8205 IMMUNOHEMATOLOGY COMPETENCY EVALUATION FORM STUDENT
More informationWhat You Will Learn in This Module: Testing for Pullorum-Typhoid Disease
Module 5-B: Testing Poultry for Pullorum-Typhoid Disease This module applies only to individuals who have attended and successfully completed an Indiana State Poultry Association NPIP Blood Testing School
More informationCells. Introduction WSBCTC 1
Cells Cells are the fundamental unit of life. All living things are composed of cells. While there are several characteristics that are common to all cells, such as the presence of a cell membrane, cytoplasm,
More informationBasic Immunologic Procedures. Complex Serological Tests
Basic Immunologic Procedures Complex Serological Tests Amal Alghamdi 2014-2015 1 Classification of antigen-antibody interactions: 1. Primary serological tests: (Marker techniques) e.g. Enzyme linked immuonosorben
More informationScott & White Institutional Biosafety Committee Compliance Program Biohazardous Material Spill Clean-Up Procedure Policy #IBC.002
I. Purpose Biohazardous material usage on the Scott & White campus is regulated by the Scott & White Institutional Biosafety Committee (IBC). Those investigators choosing to perform research with biohazardous
More informationBIOLOGY 12 - CHAPTER 11 - BLOOD - CHAPTER NOTES
BIOLOGY 12 - CHAPTER 11 - BLOOD - CHAPTER NOTES Facts Human beings are approximately 70% WATER by body weight. o Most of this water is within cells, while a smaller amount is found within: o Tissue fluid
More informationCell Biology Prokaryotic and eukaryotic cells
Cell Biology Prokaryotic and eukaryotic cells Observation of cells and organelles In this lab you will be looking at an example of a Prokaryotic cell (Bacillus cereus) and a some examples of Eukaryotic
More informationABO/Rh Blood-Typing Model:
INQUIRY & INVESTIGATION So what s happening here? is a question commonly heard when introductory science students are working on cellular or molecular laboratory concepts. Since there is a huge variety
More informationSite Care of Your Central Venous Catheter Sterile
Site Care of Your Central Venous Catheter Sterile Dressing Change Sterile Technique The skin surrounding your catheter s exit site must be kept clean, and a new sterile dressing should be applied on a
More informationBlood Lab Packet part I
Name Period Blood Lab Packet part I Lab Partner Names I understand that I am working as a group but I am to answer all of the questions. I should not copy someone else s answers. Collaboratively working
More informationBloodstains. Identification of blood, typing, and splatter
Bloodstains Identification of blood, typing, and splatter 3 questions about an 1. Is it blood? unknown stain 2. From what species did the blood originate? 3. If the blood is of human origin, how closely
More informationCare and Use of the Compound Microscope
Revised Fall 2011 Care and Use of the Compound Microscope Objectives After completing this lab students should be able to 1. properly clean and carry a compound and dissecting microscope. 2. focus a specimen
More informationABORhCard. ABORhCard Package Insert ABO and Rh Blood Grouping Device
ABORhCard Package Insert ABO and Rh Blood Grouping Device ABORhCard Intended Use The ABORhCard is a qualitative in vitro test that provides a simultaneous ABO and Rh determination of an individual s ABO
More informationHematology Morphology Critique
Survey Slide: History: 60-year-old female presenting with pneumonia Further Laboratory Data: Hgb : 90 g/l RBC : 2.92 10 12 /L Hct : 0.25 L/L MCV : 87 fl MCH : 30.8 pg MCHC : 355 g/l RDW : 17.7 % WBC :
More informationDirect Antiglobulin Test (DAT)
Exercise 8 Direct Antiglobulin Test (DAT) Objectives: 1. State the purpose for performing the DAT. 2. State what a positive DAT indicates. 3. List the reagents which are used for performing the DAT. 4.
More informationParent & Healthcare Professional Instructions for the collection of Maternal & Umbilical Cord Blood
Parent & Healthcare Professional Instructions for the collection of Maternal & Umbilical Cord Blood 1 2 3 Contents List of Umbilical Cord Blood Collection Kit Thermally insulated transportation box - do
More informationMicroscopy and Cellular Morphology
Microscopy and Cellular Morphology As we discussed in class, many organisms on the planet exist as single cells and are referred to as microorganisms bacteria, protozoans, among others. When a single microorganism
More informationMANUAL PLATELET COUNT
MANUAL PLATELET COUNT Principle Whole blood is diluted with a 1% ammonium oxalate solution. The isotonic balance of the diluent is such that all erythrocytes are lysed while the leukocytes, platelets,
More informationBLOOD Dr. Ali Ebneshahidi
BLOOD Dr. Ali Ebneshahidi Functions of blood 1. Transport of substances: like Oxygen, CO2, nutrients, waste, heat, and hormones. 2. Protection: maintenance of normal Ph, normal body fluid volume, hemostasis,
More informationBio 20 Chapter 11 Workbook Blood and the Immune System Ms. Nyboer
Bio 20 Chapter 11 Workbook Blood and the Immune System Ms. Nyboer Name: Part A: Components of Blood 1. List the 3 plasma proteins and describe the function of each Albumins osmotic balance Globulins antibodies,
More informationEDUCATIONAL COMMENTARY - GRANULOCYTE FORMATION AND CHRONIC MYELOCYTIC LEUKEMIA
LEUKEMIA Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain FREE CME/CMLE credits click on Earn CE Credits under Continuing Education
More informationUsing Blood-Typing to Determine Causes of Death in Surgery Patients Kim Williamson, East Clinton High School, Lees Creek, OH
INTRODUCTION To close the yellow note, click once to select it and then click the box in the upper left corner. To open the note, double click (Mac OS) or right click (Windows) on the note icon. Using
More informationBlood & Hematology. The human body is made up mostly of water; ~60-65% (40 L)
Blood & Hematology The human body is made up mostly of water; ~60-65% (40 L) Body s transport system plays key role in balancing fluids in the body s compartments river of life Marieb strictly speaking,
More informationAppendix J IBC Biohazard Spill Management Plan
OVERVIEW Prevention is the most important part of any spill management plan. Be sure to read and understand standard operating procedures (SOP s) and protocols for safe manipulation of biohazards before
More informationSerotyping Techniques
Serotyping Techniques Thomas A. Kruzel, M.T., N. D. Southwest College of Naturopathic Medicine & Health Sciences ABO Blood Groups Blood Group RBC Antigens Serum Antibodies Percentage O none Anti A & B
More informationA Guide to Managing Your Biological Waste at the University at Albany
A Guide to Managing Your Biological Waste at the University at Albany Section 1 - What you need to know: Definition: "Regulated Medical Waste (RMW) shall mean any of the following waste which is generated
More informationCHAPTER 10 BLOOD GROUPS: ABO AND Rh
CHAPTER 10 BLOOD GROUPS: ABO AND Rh The success of human blood transfusions requires compatibility for the two major blood group antigen systems, namely ABO and Rh. The ABO system is defined by two red
More informationLESSON ASSIGNMENT. Collection of Blood and Preparation of Blood Smears. After completing this lesson, you should be able to:
LESSON ASSIGNMENT LESSON 3 Collection of Blood and Preparation of Blood Smears. TEXT ASSIGNMENT Paragraphs 3-1 through 3-8. LESSON OBJECTIVES After completing this lesson, you should be able to: 3-1. Select
More informationSEED Haematology. Sysmex Educational Enhancement and Development February 2013
SEED Haematology Sysmex Educational Enhancement and Development February 2013 The role of the peripheral blood smear in the modern haematology laboratory Automated haematology cell counting The laboratory
More informationMICROSCOPY. To demonstrate skill in the proper utilization of a light microscope.
MICROSCOPY I. OBJECTIVES To demonstrate skill in the proper utilization of a light microscope. To demonstrate skill in the use of ocular and stage micrometers for measurements of cell size. To recognize
More informationPATIENT GUIDE. Care and Maintenance Drainage Frequency: Max. Drainage Volume: Dressing Option: Clinician s Signature: Every drainage Weekly
PATIENT GUIDE Care and Maintenance Drainage Frequency: Max. Drainage Volume: Dressing Option: Every drainage Weekly Clinician s Signature: ACCESS SYSTEMS Pleural Space Insertion Site Cuff Exit Site Catheter
More informationMALARIA PARASITE COUNTING
VERSION 1 EFFECTIVE DATE: 01/01/2016 MALARIA PARASITE COUNTING MALARIA MICROSCOPY STANDARD OPERATING PROCEDURE MM-SOP-09 1. PURPOSE AND SCOPE To describe the procedure for counting malaria parasites on
More informationRadiation Sciences Nuclear Medicine Technology Program Hot Lab Log Book (Material)
Radiation Sciences Nuclear Medicine Technology Program Hot Lab Log Book (Material) Table of Content Receiving a Radioactive Package Constancy Test Daily Survey Weekly Wipes Accuracy Test Linearity Geometric
More informationRh Immune Globulin Workup (RhIgW)
Exercise 10 Rh Immune Globulin Workup (RhIgW) 1. State the purpose for giving Rh Immune Globulin (RhIg). 2. State the population which is most frequently given RhIg. 3. State the severity of HDFN. 4. State
More informationTo see how this data can be used, follow the titration of hydrofluoric acid against sodium hydroxide below. HF (aq) + NaOH (aq) H2O (l) + NaF (aq)
Weak Acid Titration v120413 You are encouraged to carefully read the following sections in Tro (2 nd ed.) to prepare for this experiment: Sec 4.8, pp 158-159 (Acid/Base Titrations), Sec 16.4, pp 729-43
More informationPaper Chromatography: Separation and Identification of Five Metal Cations
Paper Chromatography: Separation and Identification of Five Metal Cations Objectives Known and unknown solutions of the metal ions Ag +, Fe 3+, Co 2+, Cu 2+ and Hg 2+ will be analyzed using paper chromatography.
More informationAnimal & Plant Cell Slides
Animal & Plant Cell Slides Category: Biology Type: Class Experiment, 60 min class Materials: 2 Glass Slides 2 Cover Slips 1 Bottle of methylene blue (optional) 1 Plastic tray 1 Bottle of iodine 1 Plastic
More informationIn order to be useful, a smear must have the following qualities:
Smear Preparation and Simple Stain Objectives: Make bacterial smear slides (usually called smears) Distinguish cells on these slides using a simple stain procedure Unstained microbial cells are nearly
More information