TEST SPECIFICATION GUIDE

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1 TEST SPECIFICATION GUIDE The Test Specification Guide will be available to CML HealthCare CCC s / POCC s, and to all CML clients upon request (electronically and/or hard copy). This guide outlines the information needed to access the services provided by CML Healthcare for the procurement of laboratory specimens. Each individual test listing is arranged in a consistent format, providing specific information. This guide provides the following information: Test name, synonyms or other common names for the test and the computer testing code. Patient preparation, including patient care instruction prior to, or during specimen collection, or performance of the test. Patient clinical information that is required because of its relevance to the determination of the diagnosis, and to the testing protocol. The clinical information includes, but is not limited to, patient history, date of birth, sex, ethnic background, height and weight. Specimen collection instructions, including specimen type, container or vacutainer tube, specific days and times for sample procurement. Post specimen collection instructions including storage and transportation instructions, testing facility, estimated time for test results availability, and billing information. Unless specified otherwise, specimen storage and transport is at room temperature. TSG GENERAL INFORMATION Page 1 of 6 CML HealthCare Inc Test Specification Guide Version: April-2015 DOI: Sept/2005

2 SPECIMEN PROCESSING INFORMATION Tests are listed in the manual under the following headings: TEST: The test is listed first by its most common standard nomenclature and underneath any alternate names. Each test request is specifically cross-referenced. CODE: The test code(s) must always be Data Entered unless otherwise specified. SPECIMEN REQUIREMENT: Blood test requests are indicated as Serum, Plasma, or Blood. Instructions will specify either minimum volume required or centrifuge only. When a minimum volume amount is indicated, the vacutainer tube must be centrifuged, and an aliquot separated into a plastic transport tube. BILLING: All tests are considered OHIP or non-ohip payable. Tests indicated with OHIP are covered by OHIP and are patient payment exempt upon presentation of a valid Ontario Health Card. Tests indicated with a dollar amount after the test, require patient payment before specimen collection. TSG GENERAL INFORMATION Page 2 of 6 CML HealthCare Inc Test Specification Guide Version: April-2015 DOI: Sept/2005

3 LOC: The laboratory, which performs the test, is designated by a unique abbreviation. Abbreviation Testing Facility Testing Facility Phone # BAGL Bay Area Genetic Laboratory CENTO Centogene AG CML CML HealthCare CVH Credit Valley Hospital /4214 DYN Dynacare HLRC Hamilton Lab Reference Center HOSP Designated Hospital HRL Hemostasis Reference Laboratory x KGH Kingston General Hospital LHSC London Health Services Center ext LL Life Labs LLG LifeLabs Genetics MSH Mount Sinai Hospital MUMC McMaster University Medical Centre x NAT Natera Inc NYGH North York General Hospital OGH Oshawa General Hospital PHL Public Health Labs PLSI Phenomenome Lab Services Inc SBH Sunnybrook Health Science Centre SKH Hospital for Sick Kids SMH St. Michael s Hospital SJH St. Joseph s Hospital TGH Toronto General Hospital VTF Various Testing Facilities TSG GENERAL INFORMATION Page 3 of 6 CML HealthCare Inc Test Specification Guide Version: April-2015 DOI: Sept/2005

4 LOCATION INDEX ON REPORTS LOCATION NAME ADDRESS FACILITY CODE CML HEALTHCARE MAIN LABORATORY 6560 KENNEDY ROAD, MISSISSAUGA L5T 2X4 70 MOUNT SINAI HOSPITAL 600 UNIVERSITY AVENUE, TORONTO M5G 1X5 82 UNIVERSITY HEALTH NETWORK (TORONTO GENERAL SITE) 190 ELIZABETH AVENUE, TORONTO M5G 2C4 83 NATERA INC INDUSTRIAL ROAD, SAN CARLOS, CA NORTH YORK GENERAL HOSPITAL 4001 LESLIE STREET, TORONTO M2K 1E1 84 LAKERIDGE HEALTH CORPORATION 1 HOSPITAL COURT, OSHAWA L1G 2B9 85 CREDIT VALLEY HOSPITAL 2200 EGLINTON AVE. W., MISSISSAUGA L5M 2N1 86 SUNNYBROOK HEALTH SCIENCE CENTRE 2075 BAYVIEW AVENUE, TORONTO M4N 3M5 87 PUBLIC HEALTH LAB TORONTO BRANCH 81 RESOURCE ROAD, TORONTO M9P 3T1 90 GAMMA DYNACARE 245 PALL MALL STREET, LONDON N6A 1P4 92 ST MICHEAL S HOSPITAL 30 BOND ST, TORONTO, ONT M5B 1W8 93 LIFE LABS 100 INTERNATIONAL BLVD, TORONTO M9W 6J6 94 HAMILTON LAB REFERENCE CENTRE 50 CHARLTON AVE. E., HAMILTON L8N 4A6 95 HEMOSTASIS REFERENCE LABORATORY 711 CONCESSION ST, 15(H) WING, 2 ND FL L8V 1C3 70 PHENOMENOME LABORATORY SERVICE INC. BAY AREA GENETIC LABORATORY CENTOGENE AG DOWNEY ROAD, SASKATOON, SASKATCHEWAN 205B-565 SANATORIUM ROAD, SIR WILLIAM OSLER BLDG, HAMILTON SCHILLINGALLEE 68, ROSTOCK, GERMANY S7N 4L8 L9C 7N TSG GENERAL INFORMATION Page 4 of 6 CML HealthCare Inc Test Specification Guide Version: April-2015 DOI: Sept/2005

5 LIST OF TESTS MIDWIVES ARE PERMITTED TO ORDER (TESTS FOR MOTHERS): CHEMISTRY/RIA HEMATOLOGY BACTERIOLOGY - Glucose - W.B.C differential count Antibiotic Sensitivity - Glucose Challenge, (includes R.B.C Morphology - Chlamydia Gestational Screen and platelet estimate) - Culture Cervical, - Urinalysis Routine - W.B.C (lkc count, excluding Vaginal (includes G.C) Chemical whole blood manual method) - Culture Other swabs - Urinalysis - Hematocrit or pus Microscopic - Hemoglobin examination - Sickle cell solubility test - Culture Urine - Estriol (screen) - Virus Isolation - HCG - Kleihauer - Wet preparation (for - Hepatitis associated antigen or antibody - Blood Group per antigen fungus, tricomonas, parasites) immunoassay - Strep B rapid screen - Alphafetoprotein Screen - Albumin Quantitative - Serum Ferritin - Serum Folate CYTOLOGY IMMUNOLOGY IMMUNOHEMATOLOGY - Cervicovaginal specimens - Pregnancy test - Virus antibodies hemagglutination inhibition or ELISA technique - Non-cultural, indirect antibody or antigen assays by fluorescence, agglutination or ELISA technique (toxoplasmosis) - HTLVIII/LAV antibody screen by ELISA technique (HIV Antibody) - Antibody Identification Incomplete antibody - Antibody screen - Blood group ABO and Rho (D) - Direct Anti-human globulin test - Direct Anti-human globulin test TSG GENERAL INFORMATION Page 5 of 6 CML HealthCare Inc Test Specification Guide Version: April-2015 DOI: Sept/2005

6 LIST OF TESTS MIDWIVES ARE PERMITTED TO ORDER (TESTS FOR NEWBORNS): CHEMISTRY/RIA HEMATOLOGY IMMUNOHEMATOLOGY - Bilirubin Total - W.B.C differential count - Blood group ABO and - Bilirubin Conjugated (includes R.B.C Rho (D) - Glucose Morphology and platelet - TSH/PKU Newborn estimate) screening - Platelet count - W.B.C (lkc count, excluding whole blood manual method) - Hematocrit - Hemoglobin LIST OF TESTS MIDWIVES ARE PERMITTED TO ORDER (TESTS FOR FATHERS/DONORS): CHEMISTRY/RIA HEMATOLOGY IMMUNOHEMATOLOGY - Urinalysis microscopic examination - Sickle cell solubility test (screen) - Blood group ABO and Rho (D) - Hepatitis associated antigen or antibody immunoassay - Blood group per antigen BACTERIOLOGY - Antibiotic Sensitivity - Chlamydia - Culture other swabs or pus - Virus isolation - Wet preparation (for fungus, trichomonas, parasites) IMMUNOLOGY - HTLVIII/LAV antibody screen by ELISA technique (HIV Antibody) TSG GENERAL INFORMATION Page 6 of 6 CML HealthCare Inc Test Specification Guide Version: April-2015 DOI: Sept/2005

7 3A/G RATIO (ALBUMIN/ GLOBULIN RATIO) Refer to ALBUMIN/GLOBULIN RATIO A1C (GLYCOSYLATED HEMOGLOBIN) (HbA1C) (HEMOGLOBIN A1C) Refer to HEMOGLOBIN A1C ABO, RhD (ABO & TYPE) (BLOOD GROUP & RhD) (BLOOD GROUP) (Rh TYPING) Refer to BLOOD GROUP ABO, Rh(D), GENOTYPE (BLOOD GROUP, Rh(D) & GENOTYPE) (GENOTYPE) E.G. ANTIGENS C, E, c, e Refer to BLOOD GROUP PHENOTYPE ABO & ANTIBODY SCREEN (ABO & SCREEN) (PRENATAL SCREEN) (TYPE & SCREEN) (BLOOD GROUP PRENATAL ANTIBODY) Refer to BLOOD GROUP and Refer to ANTIBODY SCREEN ACE (ANGIOTENSIN CONVERTING ENZYME) Refer to ANGIOTENSIN CONVERTING ENZYME ACETAMINOPHEN 079A Serum PLAIN RED OHIP HLRC (TYLENOL) Minimum Volume required: 2 ml Record time in hours that have elapsed between last dose and specimen collection. TAT 5 days ACETONE 002 Serum GOLD SST OHIP DYN (KETONES). Do not open tube Refrigerate during storage and transport. TAT 8 days ACETONE, QUALITATIVE Urine OHIP CML (KETONES QUALITATIVE) 10 ml random urine Submit in a YELLOW cap conical tube. TAT 1 day TEST SPECIFICATION GUIDE - SECTION A Page 1 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

8 ACETYLCHOLINE 9144 Serum GOLD SST UNINSURED HLRC RECEPTOR ANTIBODY TAT 30 days ACETYL CHOLINESTERASE 057R Red cells 2 LAVENDER OHIP DYN (RBC CHOLINESTERASE) Centrifuge tubes within 1-hour of collection Aliquot and discard plasma from lavender tubes Send red cells only Keep tubes together with an elastic Store and transport refrigerated TAT 7 days ACETYLSALICYLIC ACID (ASA) (ASPIRIN) (SALICYLATE) Refer to SALICYLATE ACYLCARNITINE 9341 Centrifuge, separate into transfer tube GREEN UNINSURED HLRC (FRACTIONATION) and freeze immediately. Store and send frozen ACID FAST BACILLUS (AFB) (MYCOBACTERIA TUBERCULOSIS DETECTION) (T.B. CULTURE) (TUBERCULOSIS CULTURE) Refer to MYCOBACTERIA TUBERCULOSIS DETECTION ACID PHOSPHATASE, PROSTATIC TEST NO LONGER AVAILABLE ACID PHOSPHATASE TOTAL TEST NO LONGER AVAILABLE ACTH (ADRENOCORTICOTROPIC HORMONE) (CORTICOTROPIN) Refer to CORTICOTROPIN ACTIVATED PROTEIN C 9901 Plasma LIGHT BLUE UNINSURED HLRC RESISTANCE Minimum Volume required: 2 ml (APCR) Patient should not be on anticoagulant therapy FREEZE PLASMA AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines TAT 25 days TEST SPECIFICATION GUIDE - SECTION A Page 2 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

9 ACUTE LEUKEMIA PHENOTYPING (LYMPHOCYTE MARKERS, T & B CELLS) (LYMPHOPROLIFERATIVE DISEASE PHENOTYPING) ACUTE RUBELLA (RUBELLA VIRUS ANTIBODY, IGM) Refer to LYMPHOCYTE MARKERS, T & B CELLS Refer to RUBELLA VIRUS ANTIBODY, IgM ADAMTS Both Red and Blue vacutainers are required. PLAIN RED N/C MUMC (THROMBOTIC THROMBOCYTOPENIC Centrifuge, separate serum and plasma AND LIGHT BLUE PURPURA) into separate transfer tubes and freeze both ASAP. Store and send frozen. FORM AVAILABLE ON CML WEBSITE ADENOVIRUS ANTIBODY SEROLOGY NO LONGER AVAILABLE ADENOVIRUS PCR 9068 Specimen must be sent on dry ice. LAVENDER UNINSURED SKH A completed molecular microbiology requisition must be sent with specimen. (See also Ministry of Health guidelines) FORM AVAILABLE ON CML WEBSITE ADIPONECTIN Serum GOLD SST UNINSURED LL Patient must be fasting for min of 8 hours Allow 30 mins for sample clot. Spin and separate, aliquot into transfer tube. Store and ship refrigerated. TAT 14 days. FORM AVAILABLE ON CML WEBSITE ADH (ANTI DIURETIC HORMONE) (ADH VASOPRESSIN) (VASOPRESSIN) Refer to VASOPRESSIN ADRENAL ANTIBODIES 9904 Serum GOLD SST OHIP HLRC ADRENOCORTICOTROPIC HORMONE (ACTH) (CORTICOTROPIN) Refer to CORTICOTROPIN TEST SPECIFICATION GUIDE - SECTION A Page 3 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

10 AFB (ACID FAST BACILLUS) (MYCOBACTERIA TUBERCULOSIS DETECTION) (T.B. CULTURE) (TUBERCULOSIS CULTURE) Refer to MYCOBACTERIA TUBERCULOSIS DETECTION AGGLUTINATION REACTION SCREEN (COLD AGGLUTININS SCREEN) Refer to COLD AGGLUTININS SCREEN AIDS (HIV) (HIV 1 & 2 ANTIBODY SCREEN) (HIV SEROLOGY) Refer to HIV 1 & 2 ANTIBODY SCREEN AGA (ANTI GLIADIN ANTIBODY) (GLIADIN ANTIBODIES) Refer to GLIADIN ANTIBODIES ALA (AMINOLEVULINATE) (AMINO LEVULINIC ACID) Refer to PROPHYRIN PRECURSORS ALANINE AMINO 223 Serum GOLD SST OHIP CML TRANSAMINASE (ALT) (SGPT) TAT 1 day ALBUMIN 005 Serum GOLD SST OHIP CML TAT 1 day ALBUMIN, QUALITATIVE Urine OHIP CML (PROTEIN, TOTAL QUALITATIVE) 10 ml random urine Submit in a YELLOW cap conical tube. TAT 2 days ALBUMIN QUANTITATIVE (MICROALBUMIN) ALBUMIN/GLOBULIN RATIO (A/G RATIO) Refer to MICROALBUMIN TEST NO LONGER AVAILABLE ALCOHOLS (GC) 9242 Whole Blood GRAY OHIP HRLC Includes Methanol, Ethanol, Acetone, Isopropanol Do not open tube. Do not separate. Use iodine swab to cleanse venepuncture site. This test is not available for CCC use. This test is only for use at Kennedy Road for hospital patients TAT 4 days TEST SPECIFICATION GUIDE - SECTION A Page 4 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

11 ALCOHOL- ETHYL (ETHANOL) Refer to ETHANOL ALCOHOL- ISOPROPYL (ISOPROPANOL) Refer to ISOPROPANOL ALCOHOL- METHYL (METHANOL Refer to METHANOL ALDOLASE TEST NO LONGER AVAILABLE ALDOSTERONE 300 Serum GOLD SST OHIP HLRC and aliquot to transfer tube. Ship frozen TAT 24 days ALDOSTERONE 300U 24-Hour Urine OHIP DYN 50 ml aliquot submit in a 90 ml white cap container No preservative Patient must be on normal sodium intake and not receiving diuretics for one week before urine sample is collected. State total 24-hour volume on the OHIP Requisition, on the specimen container and in Notes & Instructions. Retain a duplicate 50 ml sample in the freezer until test is reported. FREEZE URINE AND SEND FROZEN Refer to the General Information Pages for Specimen Processing & Transport Guidelines TAT 14 days ALKALINE PHOSPHATASE 191 Serum GOLD SST OHIP CML (PHOSPHATASE ALKALINE) (ALP) TAT 1 day ALKALINE PHOSPHATASE 191 Serum 2 GOLD SST OHIP CML FRACTIONATION 192 Label 1 SST autochem (ALKALINE PHOSPHATASE Label 1 SST Alk. Phos. Fract. ISOENZYME) (PHOSPHATASE ALKALINE ISOENZYMES) Testing Includes Total Alkaline Phosphase TAT 4 days ALLERGIC ALVEOLITIS 9036 Serum GOLD SST OHIP HLRC (ALLERGIC LUNG) (FARMERS LUNG) Do not confuse with Avian Precipitins Includes M. Faeni and T Vulgaris. To order Allergic Lung Serology please order both Farmer s Lung Precipitins (SFAR) AND Aspergillus Precipitins (SASPP) TAT 30 days TEST SPECIFICATION GUIDE - SECTION A Page 5 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

12 ALLERGY TESTING See chart Serum GOLD SST UNINSURED HRL (ASIA) Min Volume Required: 1ml (SERUM ALLERGEN TEST) Centrifuge and aliquot. (ALLERGEN SPECIFIC IGE Store and ship refrigerated. ANTIBODY TEST) Be specific when free texting allergen name. (RAST) Can enter up to nine allergens on one accession. (ALLERGEN SPECIFIC IMMUNOASSAY) TAT 5 days NOTE: TAT for unlisted allergens is 4-6 weeks. Uncommon/unlisted allergens should be followed up by contacting the Pre-Analytical Department to ensure that testing can be done prior to accessioning. Ensure the requested allergen is for diagnostic use. Research allergens are not available. Test Name Test Code Allergy Testing-First Allergen Allergy Testing-Second Allergen Allergy Testing-Third Allergen Allergy Testing-Fourth Allergen Allergy Testing-Fifth Allergen Allergy Testing-Sixth Allergen Allergy Testing-Seventh Allergen Allergy Testing-Eighth Allergen Allergy Testing-Nineth Allergen TEST SPECIFICATION GUIDE - SECTION A Page 6 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

13 ALLERGY TESTING MIX See Chart Serum GOLD SST UNINSURED HRL Centrifuge and aliquot Store and ship refrigerated Can enter up to four allergen mixes on one accession. Eg: Tree mix, Food mix, Grass mix TAT 5 days Test Name Test Code Allergy Testing- Mix Allergy Testing- Mix Allergy Testing- Mix Allergy Testing- Mix ALPHA 1-ANTITRYPSIN 555 Serum GOLD SST OHIP CML TAT 2 days ALPHA 1 ANTITRYPSIN 9905 Serum GOLD SST UNINSURED HLRC PHENOTYPE Minimum volume required: 1 ml FREEZE SERUM AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines Note: Alpha-1 Antitryspin Phenotyping Analysis is only available if previously measured alpha-1 antitrypsin was <1.5 g/l or patient is first-degree relative or spouse of known individual. Request must specify previous alpha-1 antitrypsin result and relationship for testing to proceed TAT 60 days ALPHA 1 ACID 9923 Serum GOLD SST OHIP HLRC GLYCOPROTIEN Centrifuge and aliquot to transfer tube. TEST SPECIFICATION GUIDE - SECTION A Page 7 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

14 ALPHA 2-MACROGLOBULIN 556 Serum GOLD SST OHIP HLRC Centrifuge Only. TAT 20 days ALPHA 2 PLASMIN INHIBITOR 9258 Plasma LIGHT BLUE UNINSURED HLRC (ALPHA 2 ANTIPLASMIN) Centrifuge and aliquot Platelet Poor Plasma To transfer tube. Freeze immediately. Store and ship frozen TAT 25 days ALPHA FETOPROTEIN, 691 C Serum GOLD SST OHIP CML ONCOLOGY (AFP-ONCOLOGY) Specify if testing is tumor related Diagnosis must be indicated TAT 1 day ALPHA FETOPROTEIN, 691 P Serum GOLD SST OHIP VTF PREGNANCY (AFP-PREGNANCY) For risk assessment of open neural tube defects Testing is recommended at 16 weeks gestation Completed "Maternal Serum Screen Form must be provided by ordering Physician. Indicate on the form "AFP ONLY" Results will be reported directly to the requesting Physician by the testing location. TAT 5 days ALT (ALANINE AMINO TRANSAMINASE) (SGPT) Refer to ALANINE AMINO TRANSAMINASE ALUMINUM 9355 Plasma ROYAL BLUE UNINSURED HLRC Centrifuge and aliquot plasma into K2 EDTA Aliquot tube. Separate and refrigerate As soon as possible. TEST SPECIFICATION GUIDE - SECTION A Page 8 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

15 ALUMINUM 24HR URINE hr urine ACID WASHED CONTAINER UNINSURED LL Patient must avoid chocolate, fruits, juice, beer, coffee, teas and antactids for containing aluminium 24 hours PRIOR to and during collection of 24 hour urine. 24 hour urine MUST be collected in ACID WASHED container Record total volume and transfer 20 ml of measured 24hr urine into A labelled sterile urine container and cap tightly Store and ship refrigerated. TAT 5 days ALUMINUM RANDOM URINE Random Urine ACID WASHED CONTAINER UNINSURED LL Minimum voume: 10mL Patient must avoid gadolinium-based Contrast media 48 hours prior to collection Collect urine in a labelled sterile 90ml container and Transfer WITHOUT DELAY into a labelled ACID WASHED container. Store and ship refrigerated TAT 1-2 weeks ALZ-ID 4105 Serum PLAIN RED UNINSURED PLSI (ALZHEIMER S DISEASE) Minimum volume: 1.0mL (ALZID) Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Transfer an aliquot of serum to a labelled tube, cap tightly Store and ship refrigerated at 2-8 o C TAT 1-2 weeks AMINOLEVULINATE AMETHOPTERIN (METHOTREXATE) Refer to PORPHYRIN PRECURSORS Refer to METHOTREXATE AMIKACIN Serum PLAIN RED OHIP HLRC Minimum Volume required: 1 ml PEAK 304AP Collect 'peak' specimen 30 minutes after IV infusion or 1-2 hours after IM injection by physician TROUGH 304AT Trough before IV / IM injection by physician Record time in hours that have elapsed between last dose and specimen collection. Refrigerate during storage and transport. AMIKACIN - RANDOM 304AR Serum PLAIN RED OHIP HLRC Minimum Volume required: 1 ml Specimens submitted as peak or trough are preferred; random orders should be avoided whenever possible. Store and ship refrigerated TEST SPECIFICATION GUIDE - SECTION A Page 9 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

16 AMINO ACIDS (METABOLIC SCREEN) Refer to METABOLIC SCREEN AMINO ACIDS-QUANTITATIVE 013 Plasma GREEN OHIP HLRC (AMINO ACID FRACTIONATION) Minimum Volume required: 1 ml - with Heparin (PHENYLALANINE) Fasting specimen preferred State age of patient, (date of birth), and clinical diagnosis State if patient is on a special diet FREEZE PLASMA AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines. TAT 30 days AMINO ACIDS-QUANTITATIVE 013U AMINOGLYCOSIDES AMINOPHYLLINE (THEOPHYLLINE) (UNIPHYL) REFER TO METABOLIC SCREEN Amikacin, Gentamycin or Tobramycin. See individual listings. Refer to THEOPHYLLINE AMIODARONE 9417 Plasma GREEN UNINSURED HLRC Minimum Volume required: 3 ml with Heparin Draw 1-hour prior to next dose TAT 20 days AMITRIPTYLINE 079AM Serum ROYAL BLUE OHIP DYN (ELAVIL) Minimum Volume required: 2 ml - No Additive Centrifuge and aliquot into serum tube Collect specimen hours after last dose Record time in hours that has elapsed between last dose and specimen collection. Refrigerate during storage and transport. Testing Includes Nortriptyline TAT 14 days AMMONIA (NH3) TESTING NO LONGER AVAILABLE AMOBARBITAL 9411 Serum PLAIN RED OHIP HLRC (AMYTAL) Minimum Volume required: 3 ml TEST SPECIFICATION GUIDE - SECTION A Page 10 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

17 AMOBARBITAL 9412 Urine OHIP HLRC (AMYTAL) Minimum Volume required: 10 ml random urine Submit in a 90 ml orange cap container AMOEBIC ANTIBODY 9078 Do not centrifuge tube PLAIN RED N/C PHL (E. HISTOLYTICA SEROLOGY ANTIBODY) (ENTAMOEBA HISTOLYTICA ANTIBODY) REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM AMOEBIC DETECTION Stool N/C PHL (E. HISTOLYTICA) Collect two stool samples 1 st in ova and parasite container 2 nd in 90 ml container with orange lid REQUESTING PHYSICIAN MUST PROVIDE COMPLETED PHL FORM AMOXAPINE Serum - NO LONGER AVAILABLE AMPHETAMINE 078AM Urine OHIP CML 10 ml random urine Submit in a blue cap conical tube TAT 3 days AMYLASE 018 Serum GOLD SST OHIP CML (DIASTASE) TAT 1 day AMYLASE 018U 24-Hour Urine OHIP CML (DIASTASE) 10 ml aliquot submit in a white cap conical tube No preservative State total 24-hour volume on the OHIP Requisition, on the specimen container and in Notes & Instructions. Retain a duplicate 90 ml sample in the fridge until test is reported. Testing includes urine creatinine and total volume. TAT 2 day AMYLASE 018RU Urine OHIP CML (DIASTASE) 10 ml random urine Submit in a white cap conical tube. TAT 2 days TEST SPECIFICATION GUIDE - SECTION A Page 11 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

18 AMYLASE FLUID 018FL Fluid PLAIN RED CONTRACT HLRC Minimum volume required: 1 ml This test is NOT available for CCC use. This test is only available at Kennedy Lab for hospital patients. TAT 10 days AMYLASE FRACTIONATION 018I Serum GOLD SST UNINSURED HLRC (AMYLASE ISOENZYME) Indicate clinical problem requiring analysis. TAT 45 to 60 days AMYTAL (AMOBARBITAL) Refer to AMOBARBITAL ANA (ANF) (ANTI NUCLEAR ANTIBODY) (CENTROMERE ANTIBODY) (NUCLEAR ANTIBODIES) (SLE ANTIBODIES) ANAFRANIL (CLOMIPRAMINE) Refer to NUCLEAR ANTIBODIES Refer to CLOMIPRAMINE ANCA C (CYTOPLASMIC) (ANTI NEUTROPHIL CYTOPLASMIC ANTIBODY C) (NEUTROPHIL CYTOPLASMIC ANTIBODIES) Refer to NEUTROPHIL CYTOPLASMIC ANTIBODIES - C ANCA p (PERINUCLEAR) (ANTI NEUTROPHIL CYTOPLASMIC ANTIBODIES P) Refer to NEUTROPHIL CYTOPLASMIC ANTIBODIES - P ANDROGEN TESTICULAR (TESTOSTERONE) Refer to TESTOSTERONE ANDROSTENEDIONE 305 Serum PLAIN RED OHIP SKH Spin, separate and freeze Store and ship FROZEN TAT 21 days ANDROSTERONE NO LONGER AVAILABLE TEST SPECIFICATION GUIDE - SECTION A Page 12 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

19 ANF (ANA) (ANTI-NUCLEAR ANTIBODY) (CENTROMERE ANTIBODY) (NUCLEAR ANTIBODIES) (SLE ANTIBODIES) Refer to NUCLEAR ANTIBODIES ANGIOTENSIN CONVERTING 9245 Serum GOLD SST UNINSURED HLRC ENZYME (ACE) Assay cannot be performed on a lipemic specimen Refrigerate during storage and transport. ANION GAP 053 Serum GOLD SST OHIP CML Hemolyzed specimens are unacceptable TAT 1 day ANTABUSE NO LONGER AVAILABLE ANTIBODY IDENTIFICATION HP15 Blood 3 LAVENDERS OHIP CML (ANTIBODY ID) (BLOOD GROUP ANTIBODY IDENTIFICATION) DO NOT SEPARATE Testing Includes titre if positive TAT 2 days ANTIBODY SCREEN 482 Blood LAVENDER OHIP CML (INDIRECT COOMBS) (REPEAT PRENATAL ANTIBODY SCREEN) DO NOT SEPARATE TAT 2 days ANTI CARDIOLIPIN AB (ANTI PHOSPHOLIPID) (CARDIOLIPIN ANTOBIDES) Refer to CARDIOLIPIN ANTOBIDES ANTI-CCP Refer to CYCLIC CITRULLINATED PEPTIDE ANTIBODIES ANTI dsdna ANTIBODY (ANTI-DNA) (ANTI DSDNA DOUBLE STRANDED AB) (DNA ds ANTIBODIES) Refer to DNA ds ANTIBODIES ANTI DIURETIC HORMONE (ADH) (VASOPRESSIN) Refer to VASOPRESSIN TEST SPECIFICATION GUIDE - SECTION A Page 13 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

20 ANTI ENA Refer to EXTRACTABLE NUCLEAR ANTIBODIES SCREEN (ENA ANTIBODY) (EXTRACTABLE NUCLEAR ANTIBODIES SCREEN) ANTI ENDOMYSIAL ANTIBODY (ENDOMYSIUM ANTIBODIES) Refer to ENDOMYSIUM ANTIBODIES ANTI EPIDERMAL ANTIBODY (ANTI-SKIN ANTIBODIES) (PEMPHIGUS/PEMPHIGOID ANTIBODIES) Refer to PEMPHIGUS/PEMPHIGOID ANTIBODIES ANTI GLIADIN ANTIBODY (AGA) (GLIADIN ANTIBODIES) Refer to GLIADIN ANTIBODIES ANTI GLOMERULAR Refer to GLOMERULAR BASEMENT MEMBRANE ANTIBODY BASEMENT MEMBRANE (GLOMERULAR BASEMENT MEMBRANE ANTIBODY) ANTI-GLUTAMIC ACID 9233 Serum GOLD SST OHIP HLRC DEHYDECARBOXYLASE Minimum Volume Required: 1ml (ANTI-GAD) Centrifuge and aliquot Store and ship frozen TAT - 34 days ANTI HISTONE (HISTONE ANTIBODIES) Refer to HISTONE ANTIBODIES ANTI HBs (HEPATITIS B IMMUNE STATUS) (HEAPTITIS B VIRUS SURFACE ANTIBODY) Refer to HEPATITIS B VIRUS SURFACE ANTIBODY ANTI INSULIN (INSULIN ANTIBODIES) Refer to INSULIN ANTIBODIES ANTI INTRINSIC FACTOR (INTRINSIC FACTOR ANTIBODIES) Refer to INTRINSIC FACTOR ANTIBODIES ANTI JO 1 (JO-1 EXTRACTABLE NUCLEAR ANTIBODIES) Refer to EXTRACTABLE NUCLEAR ANTIBODIES ANTI LA (SS-B) (SS-B EXTRACTABLE NUCLEAR ANTIBODIES) Refer to EXTRACTABLE NUCLEAR ANTIBODIES TEST SPECIFICATION GUIDE - SECTION A Page 14 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

21 ANTI-LKM ANTIBODY 9237 Serum GOLD SST OHIP HLRC (LKM ANTIBODY) (ANTI-LIVER KIDNEY MICROSOMAL Store and ship refrigerated ANTIBODIES) TAT 14 days ANTI-MICROSOMAL ANTIBODIES (MICROSOMAL ANTIBODIES) Refer to ANTI-THYROID PEROXIDASE ANTI MITOCHONDRIAL ANTIBODY (ASMA) (ANTI-SMOOTH MUSCLE ANTIBODIES) (MITOCHONDRIAL ANTIBODIES) (SMA) (SMOOTH MUSCLE ANTIBODY) Refer to MITOCHONDRIAL ANTIBODIES ANTIMONY RANDOM URINE Urine UNINSURED LL Min volume: 20ml Ensure hands are washed and free of contamination. For industrial exposure collect at end of work shift. Store and ship refrigerated. TAT 10 days ANTI-MULLERIAN HORMONE 9590 Serum PLAIN RED UNINSURED LL (AMH) Minium volume required: 1 ml (ANTI OVARIAN HORMONE) Centrifuge and aliquot (MIS) Store and ship frozen. TAT 10 days ANTIMYOCARDIAL ANTIBODY Serum GOLD SST UNINSURED LL Allow blood to clot for 30mins at room temp. Centrifuge. Store and ship refrigerated. TAT 6 days ANTI NEUTROPHIL CYTOPLASMIC ANTIBODIES - C (c-anca - CYTOPLASMIC) Refer to NEUTROPHIL CYTOPLASMIC ANTIBODIES - C ANTI NEUTROPHIL CYTOPLASMIC ANTIBODIES - P (p-anca PERINUCLEAR) Refer to NEUTROPHIL CYTOPLASMIC ANTOBIDIES - P ANTI NUCLEAR ANTIBODY (ANA) (ANF) (CENTROMERE ANTIBODIES) (NUCLEAR ANTIBODIES) (SLE ANTIBODIES) Refer to NUCLEAR ANTIBODIES TEST SPECIFICATION GUIDE - SECTION A Page 15 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

22 ANTI PANCREATIC ISLET CELLS ANTIBODY (PANCREATIC ISLET CELL ANTIBODIES) Refer to PANCREATIC ISLET CELL ANTIBODIES ANTI PARIETAL CELL ANTIBODIES (PARIETAL CELL ANTIBODIES) Refer to PARIETAL CELL ANTIBODIES ANTI PHOSPHOLIPID (ANTI-CARDIOLIPIN) (CARDIOLIPIN ANTIBODIES) Refer to CARDIOLIPIN ANTIBODIES ANTI-PLATELET ANTIBODIES (PLATELET ASSOCIATED ANTIBODIES) (PLATELET ANTIBODY SCREEN) Refer to PLATELET ANTIBODY SCREEN ANTI RETICULIN ANTIBODY (ANTI-RETICULIN AB) (RETICULIN ANTIBODIES) Refer to RETICULIN ANTIBODIES ANTI RNP Refer to EXTRACTABLE NUCLEAR ANTIBODIES ANTI RO (SS A) Refer to EXTRACTABLE NUCLEAR ANTIBODIES ANTI SCL 70 (Scl-70 ANTIBODIES) (SCLERODERMAL ANTIBODY) Refer to EXTRACTABLE NUCLEAR ANTIBODIES ANTI SM (ANTI SMITH) Refer to EXTRACTABLE NUCLEAR ANTIBODIES SCREEN ANTI SMOOTH MUSCLE ANTIBODIES (ANTI-MITOCHONDRIAL ANTIBODIES) (ASMA) (MITOCHONDRIAL ANTIBODIES) (SMA) (SMOOTH MUSCLE ANTIBODY) Refer to MITOCHONDRIAL ANTIBODIES ANTI SPERM ANTIBODIES (SPERM ANTIBODIES) Refer to SPERM ANTIBODIES ANTI STREPTOCCAL HYALURONIDASE ANTIBODY (ASH) TEST NO LONGER AVAILABLE ANTI STREPTOLYSIN O TITRE (ASOT) (STREPTOLYSIN O ANTIBODY) Refer to STREPTOLYSIN O ANTIBODY TEST SPECIFICATION GUIDE - SECTION A Page 16 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

23 ANTI THROMBIN III 373 Plasma LIGHT BLUE OHIP HLRC (ANTI-THROMBIN ASSAY) Minimum Volume required: 1 ml Patient should not be on anticoagulant therapy Includes both Functional and Immunological testing FREEZE PLASMA AND SEND FROZEN Refer to the General Information Page for Specimen Processing & Transport Guidelines TAT 20 days ANTI-THYROID ANTIBODY HP16A Serum GOLD SST OHIP LL (ATA) Minimum Volume Required: 2ml (ANTI-THYROID ANTIBODIES) Collect blood in SST. Allow blood to (THYROID ANTIBODY) clot at room temperature for 30 mins (THYROID ANTIBODIES) and separate by centrifugation. (THYROID AUTOANTIBODIES) Store and ship refrigerated at 2-8 degrees celcius for (THYROID AUTOANTIBODY) up to 5 days. This testing includes Anti-Thyroid Peroxidase and Anti-Thyroglobulin If longer storage is required: Transfer serum into a labelled aliquot tube, cap tightly and freeze at minus 20 degrees Celcius and ship FROZEN. This test is NOT the same as Thyroglobulin (9494) TAT 4 days ANTI THYROGLOBULIN 327 Serum GOLD SST OHIP LL (ATG) (THYROGLOBULIN ANTIBODIES) Minimum Volume required: 1.0 ml Collect blood in SST tube. Allow blood to clot at room temperature for 30 minutes and separate by centrifugation. Store and ship refrigerated at 2-8 o C for up to 7 days. If longer storage is required: Freeze sample at -20 o C. Store and ship frozen at -20 o C TAT 4 days TEST SPECIFICATION GUIDE - SECTION A Page 17 of 22 CML HealthCare Inc Test Specification Guide Version: Apr-2015

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