The Use of Stable Isotope and Molecular Technologies to Monitor MNA and Enhance In-Situ Bioremediation

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "The Use of Stable Isotope and Molecular Technologies to Monitor MNA and Enhance In-Situ Bioremediation"

Transcription

1 The Use of Stable Isotope and Molecular Technologies to Monitor MNA and Enhance In-Situ Bioremediation Eleanor M. Jennings, Ph.D. URS Corporation

2 Introduction to Technologies Stable Isotope Techniques Stable Isotope Ratios Stable Isotope Probes Molecular Techniques Denaturing Gradient Gel Electrophoresis Quantitative Polymerase Chain Reaction 2

3 3 Introduction to Isotopic Carbon Carbon comes in different weights 12 C and 13 C are most common isotopes C Carbon Approximate 98.5: 1 ratio in naturally occurring compounds and by-products of naturally occurring compounds Not radioactive working with STABLE isotopes

4 4 Introduction to Isotopic Carbon Can distinguish between 12 C, 13 C Can artificially alter the ratio of 12 C : 13 C Thus, highly inflated 13 C levels act as isotopic tag

5 5 Stable Isotope Ratio (SIR) Analyses Also called Compound Specific Isotopic Analysis (CSIA) 1, 2, 3 Dimensional CSIA Refers to the number of elements analyzed Analysis involves a single GW sample shipped to lab Takes advantage of fact that bacteria preferentially metabolize lower isotopes versus higher isotopes Causes a shift in the 13 C: 12 C ratio 13 C Sample ( ( C/ C/ C) C) Standard

6 Non-Biologically Degraded Petroleum Hydrocarbons (-30 to -20) C Less 13 C Less Biological Degradation PDB Standard More 13 C More Biological Degradation

7 7 SIR Analyses Can Differentiate Between: Biodegraded compounds versus a fresh spill Slightly biodegraded compound versus significantly biodegraded compound Compounds that have been biologically degraded versus abiotically degraded Potential sources of a contaminant, using SIR fingerprinting

8 Introduction to Stable-Isotope Probes In-situ sampling method Can confirm presence of indigenous, degrading population Utilizes isotopically-labeled target compounds Can be any carbon-based compound Ex: benzene Bio-Trap, from Microbial Insights 8

9 9 SIP: Six Simple Steps Step 1: Baiting the Beads - obtain 13 C labeled benzene, sorb 13 C labeled benzene onto beads PAC + Nomex SEM of Bio-Sep bead; 600 m 2 internal surface area / g Bio-Trap Assembly Porosity Significant surface area to sorb labeled target compound Encourages bacterial residence within beads

10 10 SIP: Six Simple Steps Step 2: Construct in-situ sampler Step 3: Deploy into MWs, suspended within the groundwater Step 4: Incubate in-situ for 6 weeks Timeframe will vary for different compounds under different site conditions Reflects actual site conditions versus laboratory microcosms Step 5: Remove from MWs Step 6: Analyze biomarkers and residual, labeled benzene

11 11 Biomarker of Choice - PLFAs High concentrations of phospholipids fatty acids (PLFAs) in microbial cell membranes High concentration of carbon in PLFAs 13 C incorporated into PLFAs microbial membrane Result of incorporation of 13 C-label from benzene into biomass Analyze using GC-IR-MS Insight into indigenous microbial ecology and activity

12 12 Quantitative Data from PLFA Analysis Total population size What groups, how many Community profile Groups, not individual species Confirmation of compound biodegradation

13 13 Quantitative Data from PLFA Analysis Which community members are responsible for degradation Information about possible environmental stress Nutritional deficits or toxic compounds Information about current microbial population In-situ conditions

14 14 Quantified Analyses From Residual Isotopic Compound Degradation rate Under in-situ conditions, not laboratory conditions Compound mineralization to CO 2 What bacteria are doing with carbon, where microbial energy is being directed Isotopic shift Changes in the ratio of 12 C : 13 C Additional confirmation of biological degradation

15 Normalized Weig TGA Analysis MTBE on PAC and Bio-Sep Bead Compounds Do Not Easily Desorb Into Environment 0.1 g benzene per BioTrap at project inception Temperatures must reach 300 o C to leach 10 mg to groundwater Temperature ( o C) 10 C/min PAC Bead

16 16 Introduction to Technologies Stable Isotope Techniques Stable Isotope Ratios Stable Isotope Probes Molecular Techniques Denaturing Gradient Gel Electrophoresis Quantitative Polymerase Chain Reaction

17 Denaturing Gradient Gel Electrophoresis Image courtesy of Microbial Insights 17

18 18 DGGE Analysis Single GW, soil sample Determine composition of mixed microbial communities Most common members More refined detail than SIP Gives actual species present Can not prove actual degradation like SIP can Thus, often used in tandem

19 19 Quantitative Polymerase Chain Reaction (qpcr) Molecular technique that quantifies a target microbial population Class of microbes or particular species Target can be as broad or specific as needed Dependent on molecular probe used Involves a single grab sample Groundwater Soil Sediment Surface water

20 Site Introduction Groundwater impacted BTEX PCE VC Source Two gasoline retail service stations Former dry cleaner site California 20

21 Direction of groundwater flow 21

22 BTEX PCE VC 22

23 23 Questions to Answer Is there an indigenous benzene degrading population? Can benzene-degrading population be further stimulated to increase degradation efficiency? Which gas station holds the dominant responsibility for the benzene in the ditch? What is the likelihood for natural attenuation of the chlorinated compounds?

24 24 Question 1 Is there an indigenous benzene degrading population? Methods used to answer: Stable Isotope Probes

25 Sampling locations for stable-isotope probes 25

26 26 Question 2 Can this population be further stimulated to increase degradation efficiency? Methods used to answer: Information from SIP, coupled to DGGE

27 Sampling locations for DGGE analyses 27

28 Question 3 Which gas station holds the dominant responsibility for the benzene in the ditch? Method to answer: Stable Isotope Ratios 28

29 Sampling locations for SIR analyses - Performed before SIP 29

30 Question 4 What is the likelihood for natural attenuation of the chlorinated compounds? Method to answer: Quantitative Polymerase Chain Reaction (qpcr) 30

31 PCE Degraders Capable of dechlorinating PCE to ethene Commonly found at Cl-solvent sites Not the only microbe that can dechlorinate Dehalococcoides sp. strain FL2 31

32 Sampling locations for qpcr analyses 32 32

33 Results 33

34 34 Stable Isotope Probe Results Presence of substantial, robust benzene-degrading population confirmed Significant incorporation of isotopic label into biomass Higher incorporation at plume periphery than in plume center Population was very active, however was showing signs of nutritional deficits both in plume center and periphery

35 35 DGGE Results Benzene Plumes Results agreed with SIP data Population in plume center Primarily sulfate-reducers Identified species associated in literature with BTEX degradation Population at plume periphery Iron-reducing bacteria Identified species associated in literature with BTEX degradation

36 36 Responsible Party? Geochem data detected BTEX from both plumes reaching ditch Question: Who was primarily responsible for impacting the ditch?

37 Sampling locations for SIR analyses 37

38 δ 13 C = -11 δ 13 C = 4 δ 13 C = 231 δ 13 C = 640 δ 13 C = 8 δ 13 C = 121 δ 13 C = 1489 δ 13 C = 187 δ 13 C =

39 δ 13 C = -11 δ 13 C = 4 δ 13 C = 231 δ 13 C = 640 δ 13 C = 8 δ 13 C = 121 δ 13 C = 1489 δ 13 C = 187 δ 13 C = 423 δ 13 C data from this service station demonstrated much higher levels of biological degradation than ditch samples 39

40 δ 13 C = -11 δ 13 C = 4 δ 13 C = 231 δ 13 C = 640 δ 13 C = 8 δ 13 C = 121 δ 13 C = 1489 δ 13 C = 187 δ 13 C = 423 δ 13 C confirmed that this service station not heavily impacting ditch 40

41 41 MNA Potential of Chlorinated Plume Needed to discern the potential to naturally attenuate the chlorinated compounds Performed qpcr to quantify Dehalococcoides population Known to fully dechlorinate PCE to ethene Coupled with DGGE analysis data

42 7.46 x 10 3 Moderate Dehalococcoides population DGGE revealed two robust dechlorinating populations Work cooperatively PCE to ethene 5.33 x

43 43 Benefits to this Project Detailed profile of microbial community members in benzene and solvent plumes SIP, DGGE, qpcr Appropriate site amendments to be developed Resulted in more rapid contaminant degradation 85% increase benzene degradation rates 56% increase PCE to ethene conversion rates Allowed site to approach site closure more rapidly Estimated years saved Substantial savings over lifespan of project

44 44 Benefits to this Project Determined which service station is primarily responsible for ditch contamination SIR Helped determine financial responsibility for ditch remediation along with site hydrogeological, geochemical data

45 45 Summary Stable isotope and molecular tools can assist with determining best remediation design Can have a significant impact on effectiveness of remediation system Can help discern most effective MNA / bioremediation strategy Determine how to most efficiently implement remediation enhancements

46 46 Summary Can determine if an MNA / bioremediation strategy is working to its full potential Monitor remediation strategy Determine new closure timeline and associated, reduced costs

47 Summary Can help demonstrate MNA / bioremediation to regulators State level: CA, MD, and other states Federal level: Multiple EPA regions Can convince regulators to sometimes allow less expensive remediation methods by proving effectiveness Can reduce lifespan of remediation project by years, reducing lifespan costs Reducing years of O&M, reporting costs $15-20K saved per year at small sites Much more at larger sites 47

48 48 Importance of Technologies Can be used on wide range of contaminants Hydrocarbons Fuel Oxygenates Chlorinated Solvents Manufacturing Wastes Chem / Pharm Materials Use is accepted by scientific community Uses backed by years of peer-reviewed research International acceptance of their use

49 Questions??? 49

FMC Environmental Solutions Peroxygen Talk January 2010 Use of Compound Specific Isotope Analysis to Enhance In Situ

FMC Environmental Solutions Peroxygen Talk January 2010 Use of Compound Specific Isotope Analysis to Enhance In Situ FMC Environmental Solutions Peroxygen Talk January 2010 Use of Compound Specific Isotope Analysis to Enhance In Situ Chemical Oxidation Performance Monitoring and Project Management In this edition of

More information

Microbiological and Geochemical Dynamics of the Subsurface: chemical oxidation and bioremediation of organic contaminants. Nora Barbour Sutton

Microbiological and Geochemical Dynamics of the Subsurface: chemical oxidation and bioremediation of organic contaminants. Nora Barbour Sutton Microbiological and Geochemical Dynamics of the Subsurface: chemical oxidation and bioremediation of organic contaminants Nora Barbour Sutton Soil Contamination Sources of Contamination Gas Stations Dry

More information

Bioremediation of contaminated soil. Dr. Piyapawn Somsamak Department of Environmental Science Kasetsart University

Bioremediation of contaminated soil. Dr. Piyapawn Somsamak Department of Environmental Science Kasetsart University Bioremediation of contaminated soil Dr. Piyapawn Somsamak Department of Environmental Science Kasetsart University Outline Process description In situ vs ex situ bioremediation Intrinsic biodegradation

More information

Bioremediation: Environmental. Groundwater remediation. Bioremediation technologies 1 / 5

Bioremediation: Environmental. Groundwater remediation. Bioremediation technologies 1 / 5 : Environmental Groundwater Remediation Robert Sherrill, C: 512-470-8710, robert.sherrill@sageenvironmental.com October 2012. ALL RIGHTS RESERVED. Revised Nov. 12, 2012 Background: or biodegradation is

More information

Tertiary Butyl Alcohol (TBA) Biodegradation. Some Frequently Asked Questions

Tertiary Butyl Alcohol (TBA) Biodegradation. Some Frequently Asked Questions Tertiary Butyl Alcohol (TBA) Biodegradation Some Frequently Asked Questions 1: What is tertiary butyl alcohol (TBA)? Tertiary butyl alcohol is a simple organic compound that contains only carbon (C), hydrogen

More information

In-situ Bioremediation of oily sediments and soil

In-situ Bioremediation of oily sediments and soil 1 Peter Werner, Jens Fahl, Catalin Stefan DRESDEN UNIVERSITY OF TECHNOLOGY In-situ Bioremediation of oily sediments and soil 2 WHAT IS OIL? MIXTURE of aliphatic and aromatic hydrocarbons Different composition

More information

Bioremediation. Biodegradation

Bioremediation. Biodegradation Bioremediation A technology that encourages growth and reproduction of indigenous microorganisms (bacteria and fungi) to enhance biodegradation of organic constituents in the saturated zone Can effectively

More information

Bioremediation of Petroleum Contamination. Augustine Ifelebuegu GE413

Bioremediation of Petroleum Contamination. Augustine Ifelebuegu GE413 Bioremediation of Petroleum Contamination Augustine Ifelebuegu GE413 Bioremediation Bioremediation is the use of living microorganisms to degrade environmental contaminants in the soil and groundwater

More information

Bioremediation. Introduction

Bioremediation. Introduction Bioremediation Introduction In the twentieth century, the ever increase in the global human population and industrialization led to the exploitation of natural resources. The increased usage of heavy metals

More information

BIOREMEDIATION: A General Outline www.idem.in.gov Mitchell E. Daniels, Jr.

BIOREMEDIATION: A General Outline www.idem.in.gov Mitchell E. Daniels, Jr. TECHNICAL GUIDANCE DOCUMENT INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT BIOREMEDIATION: A General Outline www.idem.in.gov Mitchell E. Daniels, Jr. Thomas W. Easterly Governor Commissioner 100 N. Senate

More information

Removal of diesel hydrocarbons by constructed wetlands Isotope methods to describe degradation

Removal of diesel hydrocarbons by constructed wetlands Isotope methods to describe degradation Removal of diesel hydrocarbons by constructed wetlands Isotope methods to describe degradation Watzinger A. a, Kinner P. a, Hager M. a, Gorfer M. b, and Reichenauer T.G. a a Environmental Resources and

More information

In-Situ Remediation Strategies as Sustainable Alternatives to Traditional Options. Ryan Bernesky, B.Sc., P.Ag. February 26, 2013

In-Situ Remediation Strategies as Sustainable Alternatives to Traditional Options. Ryan Bernesky, B.Sc., P.Ag. February 26, 2013 In-Situ Remediation Strategies as Sustainable Alternatives to Traditional Options Ryan Bernesky, B.Sc., P.Ag. February 26, 2013 Outline Introduction to Contaminated Sites and Remediation Strategies In-Situ

More information

CHAPTER 7: REMEDIATION TECHNOLOGIES FOR CONTAMINATED GROUNDWATER

CHAPTER 7: REMEDIATION TECHNOLOGIES FOR CONTAMINATED GROUNDWATER CHAPTER 7: REMEDIATION TECHNOLOGIES FOR CONTAMINATED GROUNDWATER There are a number of technologies that are being use to remediate contaminated groundwater. The choice of a certain remediation technology

More information

The Future of Petroleum Hydrocarbon Remediation: Site Closure Through Enhancement of In Situ Biological Degradation

The Future of Petroleum Hydrocarbon Remediation: Site Closure Through Enhancement of In Situ Biological Degradation The Future of Petroleum Hydrocarbon Remediation: Site Closure Through Enhancement of In Situ Biological Degradation Abstract By Peter Palmer, P.E., ARCADIS G&M, Inc. Sharon Hall, P.E., ARCADIS G&M, Inc.

More information

ZYMAX NEW FORENSICS ISOTOPE ANALYSIS

ZYMAX NEW FORENSICS ISOTOPE ANALYSIS ZYMAX NEW FORENSICS ISOTOPE ANALYSIS FOR EFFECTIVE SITE CLEANUP ANSWERS 2 Prepared March TABLE OF CONTENTS Page 2: ZymaX New Isotope Analysis, a Powerful Tool for Site Cleanup Decisions Page 3: Isotopic

More information

Pierre VOC Ground Water Investigation and MTBE Remediation Pilot Study

Pierre VOC Ground Water Investigation and MTBE Remediation Pilot Study Pierre VOC Ground Water Investigation and MTBE Remediation Pilot Study Joint Meeting of 2007 Eastern South Dakota Water Conference and the 52 nd Annual Midwest Ground Water Conference October 30, 2007

More information

Guidance on Remediation of Petroleum-Contaminated Ground Water By Natural Attenuation

Guidance on Remediation of Petroleum-Contaminated Ground Water By Natural Attenuation Guidance on Remediation of Petroleum-Contaminated Ground Water By Natural Attenuation Washington State Department of Ecology Toxics Cleanup Program July 2005 Publication No. 05-09-091 (Version 1.0) Geochemical

More information

In Situ Bioremediation of Chlorinated Solvent R&D 100 Award Winner

In Situ Bioremediation of Chlorinated Solvent R&D 100 Award Winner In Situ Bioremediation of Chlorinated Solvent R&D 100 Award Winner Description This patented bioremediation technology combines natural gas injection and air stripping to stimulate microbes to completely

More information

Direct Biofilm Culturing for Alberta Oil Sands Tailings Pond Water Remediation

Direct Biofilm Culturing for Alberta Oil Sands Tailings Pond Water Remediation Direct Biofilm Culturing for Alberta Oil Sands Tailings Pond Water Remediation Raymond J. Turner, PhD Professor; Biochemistry and Microbiology Microbiology Research Cluster Chair Department of Biological

More information

Advanced Tools for In Situ Remediation. Workshop

Advanced Tools for In Situ Remediation. Workshop Advanced Tools for In Situ Remediation Workshop June 22, 2011 Co-Sponsored by: 8:00 to 11:00 Registration/Check in Advanced Tools for In Situ Remediation Workshop Schedule June 22 and 23, 2011 RAM Center

More information

SiREM-Major Services/Products

SiREM-Major Services/Products Optimizing in situ Bioremediation of Chlorinated Ethenes in Ontario s Groundwater Phil Dennis, Peter Dollar, Jeff Roberts, Sandra Dworatzek Smart Remediation, Ottawa February 27, 2014 SiREM-Major Services/Products

More information

Bioremediation of Petroleum Hydrocarbons and Chlorinated Volatile Organic Compounds with Oxygen and Propane Gas infusion

Bioremediation of Petroleum Hydrocarbons and Chlorinated Volatile Organic Compounds with Oxygen and Propane Gas infusion Bioremediation of Petroleum Hydrocarbons and Chlorinated Volatile Organic Compounds with Oxygen and Propane Gas infusion Walter S. Mulica Global Technologies Fort Collins, CO Co-Authors Mike Lesakowski

More information

Advances in Biological In-Situ Remediation: Full Scale Applications in the Netherlands. Experts in environmental and sustainable biotechnology

Advances in Biological In-Situ Remediation: Full Scale Applications in the Netherlands. Experts in environmental and sustainable biotechnology Advances in Biological In-Situ Remediation: Full Scale Applications in the Netherlands NORDROCS, September 2-21, 26, Malmö First joint Nordic Meeting on Remediation of Contaminated Sites Maurice Henssen,

More information

Origin of Life. Chapter 6.1

Origin of Life. Chapter 6.1 Origin of Life Chapter 6.1 Main points Life at the Extremes! The extreme conditions that limit growth, or prove lethal to most organisms can be favorable for others! Extremes that can kill majority of

More information

Accelerated Site Cleanup Using a Sulfate-Enhanced In Situ Remediation Strategy

Accelerated Site Cleanup Using a Sulfate-Enhanced In Situ Remediation Strategy Accelerated Site Cleanup Using a Sulfate-Enhanced In Situ Remediation Strategy By: Sheri Knox, Tim Parker, & Mei Yeh YOUR NATURAL SOLUTIONS Patented Methods for In Situ Bioremediation Slide 2 About EOS

More information

Microorganisms and soil fertility. Jaap Bloem and Peter de Ruiter

Microorganisms and soil fertility. Jaap Bloem and Peter de Ruiter Microorganisms and soil fertility Jaap Bloem and Peter de Ruiter Vegetation patches Under harsh conditions plants can survive by living close together: in patches surrounded by bare soil. In fact, the

More information

Science in the Real World Microbes In Action

Science in the Real World Microbes In Action Science in the Real World Microbes In Action Edited by: Teresa Thiel, Ph.D. University of Missouri-St. Louis Program Director & Microbiologist Slick Oil Lab is a curriculum unit developed as part of the

More information

PROGRAM AT A GLANCE 12

PROGRAM AT A GLANCE 12 PROGRAM AT A GLANCE 12 Sunday, May 22, 2016 8:00 a.m. 5:00 p.m. Short Courses 3:00 9:00 p.m. Registration Desk Open 6:00 9:00 p.m. Welcome Reception, Exhibits, Poster Group 1 Display Monday, May 23, 2016

More information

Bioremediation of Sandy Soiled, Vegetated Area Contaminated by Oily Floodwaters Aug 2013

Bioremediation of Sandy Soiled, Vegetated Area Contaminated by Oily Floodwaters Aug 2013 Bioremediation of Sandy Soiled, Vegetated Area Contaminated by Oily Floodwaters Aug 2013 Overview: On August 3, an extremely heavy rainstorm fell on a Colorado natural gas processing facility causing flood

More information

N O T E S. Environmental Forensics. Identification of Natural Gas Sources using Geochemical Forensic Tools. Dispute Scenarios

N O T E S. Environmental Forensics. Identification of Natural Gas Sources using Geochemical Forensic Tools. Dispute Scenarios Environmental Forensics N O T E S V o l u m e 2 9 Identification of Natural Gas Sources using Geochemical Forensic Tools By Paul Boehm, Ph.D. and Tarek Saba, Ph.D. F o r m o r e i n f o r m a t i o n o

More information

Characterization of Natural Biodegradation Using Benzene Carbon Isotopes, LNAPL Characterization and Geochemical Lines of Evidence

Characterization of Natural Biodegradation Using Benzene Carbon Isotopes, LNAPL Characterization and Geochemical Lines of Evidence Characterization of Natural Biodegradation Using Benzene Carbon Isotopes, LNAPL Characterization and Geochemical Lines of Evidence Glenn Ulrich and Paul Feshbach-Meriney Background Site conditions Lines

More information

Microbial Fingerprinting Methods EMD Team Fact Sheet November 2011

Microbial Fingerprinting Methods EMD Team Fact Sheet November 2011 Microbial Fingerprinting Methods EMD Team Fact Sheet November 2011 This fact sheet, developed by the ITRC Environmental Molecular Diagnostics (EMD) Team, is one of 10 designed to provide introductory information

More information

An Analysis of Composting As an Environmental Remediation Technology 1EPA. 2 Printed on paper that contains at least 20 percent postconsumer fiber.

An Analysis of Composting As an Environmental Remediation Technology 1EPA. 2 Printed on paper that contains at least 20 percent postconsumer fiber. 1EPA United States Environmental Protection Agency Solid Waste and Emergency Response (5306W) EPA530-R-98-008 April 1998 An Analysis of Composting As an Environmental Remediation Technology 2 Printed on

More information

Interim Summary Report for the Treatment Facility D Helipad In-Situ Bioremediation Treatability Test

Interim Summary Report for the Treatment Facility D Helipad In-Situ Bioremediation Treatability Test Interim Summary Report for the Treatment Facility D Helipad In-Situ Bioremediation Treatability Test June 15, 2012 Environmental Restoration Department This work was performed under the auspices of the

More information

Environmental and Economical Oil and Groundwater Recovery and Treatment Options for hydrocarbon contaminated Sites

Environmental and Economical Oil and Groundwater Recovery and Treatment Options for hydrocarbon contaminated Sites 2014 5th International Conference on Environmental Science and Technology IPCBEE vol.69 (2014) (2014) IACSIT Press, Singapore DOI: 10.7763/IPCBEE. 2014. V69. 15 Environmental and Economical Oil and Groundwater

More information

INTRODUCTION TO IN SITU BIOREMEDIATION OF GROUNDWATER

INTRODUCTION TO IN SITU BIOREMEDIATION OF GROUNDWATER 542-R-13-018 December 2013 Office of Solid Waste and Emergency Response INTRODUCTION TO IN SITU BIOREMEDIATION OF GROUNDWATER EXECUTIVE SUMMARY is an engineered technology that modifies environmental conditions

More information

GUIDANCE PROTOCOL Environmental Restoration Project ER-0518

GUIDANCE PROTOCOL Environmental Restoration Project ER-0518 GUIDANCE PROTOCOL Environmental Restoration Project ER-0518 Application of Nucleic Acid-Based Tools for Monitoring Monitored Natural Attenuation (MNA), Biostimulation, and Bioaugmentation at Chlorinated

More information

Cost of In situ Treatment of Fuel Oxygenates

Cost of In situ Treatment of Fuel Oxygenates Cost of In situ Treatment of Fuel Oxygenates Linda Fiedler, U.S. EPA, Office of Solid Waste and Emergency Response, Washington, DC Michael Berman, Tetra Tech EM Inc., Reston, VA Abstract Introduction As

More information

Enhanced In Situ Biodegradation of Trichloroethene/1,1,1 Trichloroethane in Fractured Rock Groundwater

Enhanced In Situ Biodegradation of Trichloroethene/1,1,1 Trichloroethane in Fractured Rock Groundwater Enhanced In Situ Biodegradation of Trichloroethene/1,1,1 Trichloroethane in Fractured Rock Groundwater Michael S. Kozar, PG Introduction Michael Kozar, PG, LSRP Vice President, O Brien & Gere 22 years

More information

XIII. XENOBIOTICS IN SOIL

XIII. XENOBIOTICS IN SOIL XIII. XENOBIOTICS IN SOIL Required Readings: Alexander, M. 1965. Persistence and biological reactions of pesticides in soils. Soil Science Society of America Proceedings 29:1-7. Ankumah, R.O., W.A. Dick

More information

Using Alternative Energy Sources of Wind and Solar to Biosparge Groundwater

Using Alternative Energy Sources of Wind and Solar to Biosparge Groundwater 19 th International Petroleum Environmental Conference Bioremediation of Groundwater and Soils I Wednesday, October 31, 2012 Morning Session Using Alternative Energy Sources of Wind and Solar to Biosparge

More information

Environmental Forensics

Environmental Forensics Environmental Forensics N O T E S Volume 10 2011 CERCLA s Petroleum Exclusion and the Use of Chemical Forensic Methods Tarek Saba and Paul Boehm For more information on Exponent s environmental services,

More information

Compound Specific Isotope Analysis:

Compound Specific Isotope Analysis: Compound Specific Isotope Analysis: The Science, Technology and Selected Examples from the Literature with Application to Fuel Oxygenates and Chlorinated Solvents Dr. Robert J. Pirkle July 2006 Microseeps

More information

A SYSTEMS APPROACH TO IN-SITU BIOREMEDIATION: FULL SCALE APPLICATION

A SYSTEMS APPROACH TO IN-SITU BIOREMEDIATION: FULL SCALE APPLICATION A SYSTEMS APPROACH TO IN-SITU BIOREMEDIATION: FULL SCALE APPLICATION Michael Scalzi (Innovative Environmental Technologies, Inc., Mt. Laurel, New Jersey) Xandra Turner, P.E. (InterBio, The Woodlands, Texas)

More information

LABORATORY STUDIES ON THE BIOREMEDIATION OF SOIL CONTAMINATED BY DIESEL

LABORATORY STUDIES ON THE BIOREMEDIATION OF SOIL CONTAMINATED BY DIESEL International Journal of Advanced Research in Engineering and Technology (IJARET) Volume 7, Issue 1, Jan-Feb 2016, pp. 80-88, Article ID: IJARET_07_01_010 Available online at http://www.iaeme.com/ijaret/issues.asp?jtype=ijaret&vtype=7&itype=1

More information

HW10 PCBs/Pesticides Remediation 10 points Due: Nov. 21

HW10 PCBs/Pesticides Remediation 10 points Due: Nov. 21 HW10 PCBs/Pesticides Remediation 10 points Due: Nov. 21 1. [2 pts] Given the following PCBs, list the order you would predict them to be biotransformed in an aerobic and anaerobic environment (1 = fastest,

More information

Treatment Techniques of Oil-Contaminated Soil and Water Aquifers

Treatment Techniques of Oil-Contaminated Soil and Water Aquifers International Conf. on Water Resources & Arid Environment (2004) Treatment Techniques of Oil-Contaminated Soil and Water Aquifers Petroleum Engineering Department, King Saud University, P.O. Box 800, Riyadh

More information

Activated Sodium Persulfate In Situ Blending Combines Environmental Remediation and Soil Stabilization

Activated Sodium Persulfate In Situ Blending Combines Environmental Remediation and Soil Stabilization Activated Sodium Persulfate In Situ Blending Combines Environmental Remediation and Soil Stabilization 5th Conference: Innovative Environmental Assessment and Remediation Technology AIPG Georgia Section

More information

In-situ Bioremediation of Oil Contaminated Soil - Practical Experiences from Denmark

In-situ Bioremediation of Oil Contaminated Soil - Practical Experiences from Denmark In-situ Bioremediation of Oil Contaminated Soil - Practical Experiences from Denmark Anne Louise Gimsing, Jan Boeg Hansen, Erik Permild, Gry Schwarz & Erik Hansen Cleanfield, Mesterlodden 36, DK-2820 Gentofte,

More information

Partnering with Nature for a Cleaner Tomorrow

Partnering with Nature for a Cleaner Tomorrow VERDE ENVIRONMENTAL, INC. HOME OF Emergency Liquid Spill Control Verde Environmental, Inc. 9223 Eastex Freeway Houston, TX 77093 Office: 713.691.6468 Toll Free: 800.626.6598 Fax: 713.691.2331 www.micro-blaze.com

More information

Soil and Groundwater. Removing Contaminants. Groundwater. Implementing. Remediation. Technologies 1 / 6

Soil and Groundwater. Removing Contaminants. Groundwater. Implementing. Remediation. Technologies 1 / 6 carol townsend, C: 469-263-4343, carol.townsend@sageenvironmental.com robert sherrill, C: 512-470-8710, robert.sherrill@sageenvironmental.com October 2012. ALL RIGHTS RESERVED. Revised Nov. 12, 2012 Background:

More information

The Community Alliance on the Savannah River Site. HARAMBEE HOUSE, INC. Project Citizens For Environmental Justice September 2005

The Community Alliance on the Savannah River Site. HARAMBEE HOUSE, INC. Project Citizens For Environmental Justice September 2005 The Community Alliance on the Savannah River Site HARAMBEE HOUSE, INC. Project Citizens For Environmental Justice September 2005 A Scientific and Technical Review of The Use of Bioremediation as a Clean

More information

Assessment of Natural Biodegradation at Petroleum Release Sites Guidance Document 4-03

Assessment of Natural Biodegradation at Petroleum Release Sites Guidance Document 4-03 http://www.pca.state.mn.us/programs/lust_p.html Assessment of Natural Biodegradation at Petroleum Release Sites Guidance Document 4-03 This document explains how to assess the occurrence of natural biodegradation

More information

Proxy Simulation of In-Situ Bioremediation System using Artificial Neural Network

Proxy Simulation of In-Situ Bioremediation System using Artificial Neural Network Proxy Simulation of In-Situ Bioremediation System using Artificial Neural Network Deepak Kumar PhD Student Civil Engineering Department, IIT Delhi- 110016 ABSTRACT In-situ bioremediation is one of the

More information

Cost-Effective Bioremediation of Perchlorate in Soil & Groundwater

Cost-Effective Bioremediation of Perchlorate in Soil & Groundwater Cost-Effective Bioremediation of Perchlorate in Soil & Groundwater Evan Cox - GeoSyntec Consultants Elizabeth Edwards - University of Toronto Scott Neville & Michael Girard - Aerojet Outline Perchlorate

More information

Molecular Genetic Tools for Monitoring Ecosystems and the Environment

Molecular Genetic Tools for Monitoring Ecosystems and the Environment Molecular Genetic Tools for Monitoring Ecosystems and the Environment Loring (Larry) Nies School of Civil Engineering Purdue University West Lafayette, Indiana Current and Former Students: Brett. Baldwin,

More information

Contaminated Sites and Environmental Remediation

Contaminated Sites and Environmental Remediation Contaminated Sites and Environmental Remediation Larkin Kee (nee Mosscrop) Joint Presentation for WiN (Eastern Ontario Chapter) and CNS (Chalk River Branch) Outline Overview of Contaminated Sites Contaminants

More information

PROTOCOL NO. 5: Petroleum Hydrocarbon Analytical Methods and Standards

PROTOCOL NO. 5: Petroleum Hydrocarbon Analytical Methods and Standards PROTOCOL FOR THE CONTAMINATED SITES REGULATION UNDER THE ENVIRONMENT ACT PROTOCOL NO. 5: Petroleum Hydrocarbon Analytical Methods and Standards Prepared pursuant to Part 6 Administration, Section 21, Contaminated

More information

TCE. The Use & Remediation of TCE at NASA. Keep reading. is developing innovative. NASA s pollution prevention efforts significantly reduced TCE use

TCE. The Use & Remediation of TCE at NASA. Keep reading. is developing innovative. NASA s pollution prevention efforts significantly reduced TCE use National Aeronautics and Space Administration Space flight and exploration begin with developing innovative technologies here on Earth. Our commitment to environmental stewardship is central to that effort.

More information

Evalution of Methanothrophic Bacteria during Injection of Gaseous Nutrients for In Situ Trichloroethylene Bioremediation in a Sanitary Landfill

Evalution of Methanothrophic Bacteria during Injection of Gaseous Nutrients for In Situ Trichloroethylene Bioremediation in a Sanitary Landfill WSRC-MS-98-00854 Evalution of Methanothrophic Bacteria during Injection of Gaseous Nutrients for In Situ Trichloroethylene Bioremediation in a Sanitary Landfill Robin L. Brigmon, Denis J. Altman, Marilyn

More information

ENVIRONMENTAL MANAGEMENT:- BIOREMEDIATION OF POLLUTED ENVIRONMENT

ENVIRONMENTAL MANAGEMENT:- BIOREMEDIATION OF POLLUTED ENVIRONMENT Sasikumar, C.Sheela and Taniya Papinazath Environmental Management:- Bioremediation Of Polluted Environment in Martin J. Bunch, V. Madha Suresh and T. Vasantha Kumaran, eds., Proceedings of the Third International

More information

Vapor Intrusion Pathway: A Practical Guideline

Vapor Intrusion Pathway: A Practical Guideline Vapor Intrusion Pathway: A Practical Guideline John Boyer New Jersey Dept. of Environmental Protection November 2009 2 ITRC Shaping the Future of Regulatory Acceptance Host organization Network State regulators

More information

Injection of Fentons Reagent In-Situ Case studies. Introduction. Introduction. Background - Principles

Injection of Fentons Reagent In-Situ Case studies. Introduction. Introduction. Background - Principles Injection of Fentons Reagent In-Situ Case studies Introduction Höstmöte 2004 Linköping 27. Oktober 2004 The oxidant, known as Fenton s reagent, destroys a variety of industrial wastes and generates innocuous

More information

Biotechnology in Industrial Waste Treatment and Bioremediation

Biotechnology in Industrial Waste Treatment and Bioremediation INSTHTUT WAR BfoHotfceic W. A. R. Bibiiothek Wasserverscrgung, Abwassortechnik ll»v.-nr. D/fG2i^~ Abfa!!tochn;k t.;nd RatsnrJia^ving^ Technische Univarr-itM Da.Trs-.-dt PetersenstraBe 13, 6.'i237 Darmstadt

More information

The Processes of Life. Bicester Community College Science Department

The Processes of Life. Bicester Community College Science Department B4 The Processes of Life B4 Key Questions How do chemical reactions take place in living things? How do plants make food? How do living organisms obtain energy? How do chemical reactions take place in

More information

การช วบ าบ ด (Bioremediation)

การช วบ าบ ด (Bioremediation) Hazardous Organic Wastes การช วบ าบ ด (Bioremediation) Petroleum products Fungicides Insecticides Herbicides D Dalee Department of Biology Faculty of Science & Technology Yala Rajabhat University 2 Most

More information

Ulf Lindmark Litigation Support Experience

Ulf Lindmark Litigation Support Experience Case No. 1 Retained by attorneys for defendant (furniture manufacturer) in case against the property owner, who sought damages for soil and groundwater contamination beneath the property. Case No. 2 Retained

More information

Rehabilitation and Remediation of POPs

Rehabilitation and Remediation of POPs and of POPs Module 3 and Overview of the course This module will cover various remediation and rehabilitation strategies. Contents Strategies (and part of ) Summary technologies Summary Learning outcomes

More information

Analysis of Pesticide Degradation Pathways

Analysis of Pesticide Degradation Pathways Analysis of Pesticide Degradation Pathways Prof. dr. Biljana Škrbić Faculty of Technology, University of Novi Sad, Novi Sad Pesticides are widely used for the control of weeds, diseases, and pests of cultivated

More information

*:57$& In Situ Bioremediation. Technology Overview Report. Liesbet van Cauwenberghe. Diane S Roote, P.G.

*:57$& In Situ Bioremediation. Technology Overview Report. Liesbet van Cauwenberghe. Diane S Roote, P.G. Technology Overview Report GWRTAC TO-98-01 SERIES Prepared By: Liesbet van Cauwenberghe and Diane S Roote, P.G. Ground-Water Remediation Technologies Analysis Center October 1998 Prepared For: Ground-Water

More information

Guide to the Remediation Report & Fee Submission Form

Guide to the Remediation Report & Fee Submission Form Guide to the Remediation Report & Fee Submission Form May 1, 2012 TABLE OF CONTENTS 1 DEFINITIONS... 1 2 INTRODUCTION... 3 3 BACKGROUND... 3 3.1 EXISTING POLICY/GUIDELINES FRAMEWORK... 3 Domestic Fuel

More information

Contaminated Soils Treatment via Ex-Situ and In-Situ Mixing Methods

Contaminated Soils Treatment via Ex-Situ and In-Situ Mixing Methods R e g e n O x T E C H N I C A L B U L L E T I N 1 1. 0 RegenOx TM A d v a n c e d C h e m i c a l O x i d a t i o n Contaminated Soils Treatment via Ex-Situ and In-Situ Mixing Methods The treatment of

More information

Ecosystem ecology emphasizes energy flow and chemical recycling

Ecosystem ecology emphasizes energy flow and chemical recycling AP Biology Chapter 54 notes Ecosystems Ecosystem ecology emphasizes energy flow and chemical recycling An ecosystem consists of all the organisms in a community and all the abiotic factors with which they

More information

Trends in Environmental Remediation

Trends in Environmental Remediation Stephen Rosansky, P.E, PMP Trends in Environmental Remediation IX Seminário Inernacional Sobre Remediação e Revitalizaçao de Áreas Contaminadas 1 Battelle Memorial Institute Non-profit charitable trust

More information

EFFECTIVENESS OF BIOREMEDIATION PROCESS IN HYDROCARBON - CONTAMINATED SOILS

EFFECTIVENESS OF BIOREMEDIATION PROCESS IN HYDROCARBON - CONTAMINATED SOILS EFFECTIVENESS OF BIOREMEDIATION PROCESS IN HYDROCARBON - CONTAMINATED SOILS Lilija Kaldien Gražina Giedraityt Vilnius University, Lithuania Rapolas Liužinas Public Establishment Soil Remediation Technologies,

More information

Benzene Oxidation by Aerobic Respiration 7.5 O 2 + C 6 H 6 --- 6 CO 2 + 3 H 2 O 0.33 mg/l C 6 H 6 degraded per mg/l O 2 consumed

Benzene Oxidation by Aerobic Respiration 7.5 O 2 + C 6 H 6 --- 6 CO 2 + 3 H 2 O 0.33 mg/l C 6 H 6 degraded per mg/l O 2 consumed IN-SITU BIOREMEDIATION EVALUATION USING THE WATERLOO EMITTER Douglas A Sweeney, M.Sc., P.Eng. and Ian Mitchell, M.Sc., P.Geo. SEACOR Environmental Inc. INTRODUCTION Subsurface hydrocarbon impacts from

More information

PCR Polymerase Chain Reaction

PCR Polymerase Chain Reaction Biological Sciences Initiative HHMI PCR Polymerase Chain Reaction PCR is an extremely powerful technique used to amplify any specific piece of DNA of interest. The DNA of interest is selectively amplified

More information

U. S. Army Corps of Engineers Ground Water Extraction System Subsurface Performance Checklist

U. S. Army Corps of Engineers Ground Water Extraction System Subsurface Performance Checklist U. S. Army Corps of Engineers Ground Water Extraction System Subsurface Performance Checklist Installation Name Site Name / I.D. Evaluation Team Site Visit Date This checklist is meant to aid in evaluating

More information

Microbial Growth. By Dr. Carmen Rexach Microbiology Mt San Antonio College

Microbial Growth. By Dr. Carmen Rexach Microbiology Mt San Antonio College Microbial Growth By Dr. Carmen Rexach Microbiology Mt San Antonio College Microbial growth Definition Increase in the number of cells Increase in microbial mass Requires specific physical and chemical

More information

Sustainable Environmental Remediation (SER) Through Bioremediation of Petroleum Contaminated Soils* Satya Ganti, Sarva Bio Remed, LLC

Sustainable Environmental Remediation (SER) Through Bioremediation of Petroleum Contaminated Soils* Satya Ganti, Sarva Bio Remed, LLC Sustainable Environmental Remediation (SER) Through Bioremediation of Petroleum Contaminated Soils* Satya Ganti, Sarva Bio Remed, LLC Abstract Reduction in the amount of greenhouse gases (GHGs) emitted

More information

Prepared for ENRY2000, Belgrade, Yugoslavia, September 27, 2001

Prepared for ENRY2000, Belgrade, Yugoslavia, September 27, 2001 Prepared for ENRY2000, Belgrade, Yugoslavia, September 27, 2001 PERSPECTIVES ON INNOVATIVE CHARACTERIZATION AND REMEDIATION TECHNOLOGIES FOR CONTAMINATED SITES W.W. Kovalick, Jr. Technology Innovation

More information

Monitoring Bioremediation with Molecular Tools. Hauke Smidt

Monitoring Bioremediation with Molecular Tools. Hauke Smidt ecogenomics Monitoring Bioremediation with Molecular Tools Hauke Smidt Laboratory of Microbiology Wageningen University Fate of Environmental Pollutants Biodegradability largely depends on chemical structure

More information

GOLDEN ENVIRO HERBA- EXTRACT DRAIN CLOG FREE. Pleasant lemon fragrance provides instant freshness Patented microbial technology

GOLDEN ENVIRO HERBA- EXTRACT DRAIN CLOG FREE. Pleasant lemon fragrance provides instant freshness Patented microbial technology GOLDEN ENVIRO HERBA- EXTRACT DRAIN CLOG FREE Application Sheet A clogged drain can stop kitchen operations - whether it is a busy restaurant or a dinner for two at home. Drain Clog Free combines fast-

More information

Genomic DNA Extraction Kit INSTRUCTION MANUAL

Genomic DNA Extraction Kit INSTRUCTION MANUAL Genomic DNA Extraction Kit INSTRUCTION MANUAL Table of Contents Introduction 3 Kit Components 3 Storage Conditions 4 Recommended Equipment and Reagents 4 Introduction to the Protocol 4 General Overview

More information

PlumeStop Technical Bulletin 5.1: In situ Containment of PFOA and PFOS Using Plumestop Liquid Activated Carbon TM. Introduction and Background

PlumeStop Technical Bulletin 5.1: In situ Containment of PFOA and PFOS Using Plumestop Liquid Activated Carbon TM. Introduction and Background PlumeStop Technical Bulletin 5.1 In Situ Containment of PFOA and PFOS Using Plumestop Liquid Activated Carbon TM Introduction and Background Per- and polyfluorinated alkyl substances (PFAS) are a class

More information

ECORISK. Consulenze Ambientali. of Francesco Fratepietro, MChem MSc

ECORISK. Consulenze Ambientali. of Francesco Fratepietro, MChem MSc Consulenze Ambientali ECORISK Because environment is not our property of Francesco Fratepietro, MChem MSc The Consulenze Ambientali ECORISK MChem Francesco Fratepietro offers a wide range of services and

More information

1. COMMERCIAL COMPOSTING OVERVIEW 2. COMPOSTABLE VS. BIODEGRADABLE 3. ECO LABELS WHAT TO LOOK FOR

1. COMMERCIAL COMPOSTING OVERVIEW 2. COMPOSTABLE VS. BIODEGRADABLE 3. ECO LABELS WHAT TO LOOK FOR 1 COMMERCIAL COMPOSTING, GREEN-WASHING AND ECO LABELS 1. COMMERCIAL COMPOSTING OVERVIEW 2. COMPOSTABLE VS. BIODEGRADABLE 3. ECO LABELS WHAT TO LOOK FOR 2 Commercial Composting What is it? Commercial composting

More information

Forensic Fingerprinting Petroleum Contaminants using UVF Field Screening Technology

Forensic Fingerprinting Petroleum Contaminants using UVF Field Screening Technology Forensic Fingerprinting Petroleum Contaminants using UVF Field Screening Technology Grand Calumet River and Lake Michigan Steve Greason, Speaker Sitelab Corporation Technical Session (Frio): Environmental

More information

Optimization of In-Situ Injection and Bioremediation Design at a Brownfield Site

Optimization of In-Situ Injection and Bioremediation Design at a Brownfield Site Optimization of In-Situ Injection and Bioremediation Design at a Brownfield Site Howard F. Nichols III, P.E. (hnichols@trcsolutions.com), Brendan J. Lazar (blazar@trcsolutions.com), Nidal M. Rabah, PhD,

More information

Ex Centrale Gas (RA) Eni Div. Exploration & Production

Ex Centrale Gas (RA) Eni Div. Exploration & Production Ex Centrale Gas (RA) Eni Div. Exploration & Production Ex Centrale Gas (RA) Ex Central already undergone previous remediation activities Residual contamination of soils Hydrocarbons Design and execution

More information

If you want to test an idea

If you want to test an idea CONTACT For the development and demonstration of methods for the investigation and remediation of contaminated soil and groundwater If you want to test an idea the Danish Soil Partnership is here to help

More information

1 Heap Leach Mine in Central New Mexico

1 Heap Leach Mine in Central New Mexico Journal of Soil Conraminarion. 3(3):27 1-283 (1 994) Assessment of the Potential for In Situ Bioremediation of Cyanide and Nitrate Contamination at a 1 Heap Leach Mine in Central New Mexico Carleton S.

More information

Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company

Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company Biotechnology and reporter genes Here, a lentivirus is used to carry foreign DNA into chickens. A reporter gene (GFP)indicates that foreign DNA has been successfully transferred. Recombinant DNA continued

More information

Georgia Performance Standards: Science Grades Frames. Frames Tasks

Georgia Performance Standards: Science Grades Frames. Frames Tasks How to read the Frames Standards Correlations The Frames Standards Correlations include information on how you and your students can use Frames to meet your curriculum and technology standards. Since you

More information

EPA Oil Spill Research Grant Program

EPA Oil Spill Research Grant Program EPA Oil Spill Research Grant Program Mitch Lasat, Ph.D. National Center for Environmental Research Role of the National Center for Environmental Research (NCER) - Manage EPA/ORD extramural research grants

More information

Green Remediation via an Enhanced In Situ Bioremediation Solar-Powered System

Green Remediation via an Enhanced In Situ Bioremediation Solar-Powered System C-041, in K.A. Fields and G.B. Wickramanayake (Chairs), Remediation of Chlorinated and Recalcitrant Compounds 2010. Seventh International Conference on Remediation of Chlorinated and Recalcitrant Compounds

More information

In Situ and Ex Situ Bioremediation Technologies for Groundwater Contaminated with Chlorinated Solvents

In Situ and Ex Situ Bioremediation Technologies for Groundwater Contaminated with Chlorinated Solvents In Situ and Ex Situ Bioremediation Technologies for Groundwater Contaminated with Chlorinated Solvents Michelle M. Lorah, U.S. Geological Survey Emily Majcher, Duane Graves, Geosyntec Consultants John

More information

Impact of functional microbial diversity on soil ecosystem services and assessment thereof

Impact of functional microbial diversity on soil ecosystem services and assessment thereof Impact of functional microbial diversity on soil ecosystem services and assessment thereof, Anders Johansen, Niels Bohse Hendriksen Department of Environmental Science, AU, Roskilde, Denmark UNI VERSITET

More information

1. PUBLIC HEALTH STATEMENT

1. PUBLIC HEALTH STATEMENT 1 This Statement was prepared to give you information about fuel oils and to emphasize the human health effects that may result from exposure to them. The Environmental Protection Agency (EPA) has identified

More information

MOLECULAR GENETICS GENETIC ENGINEERING RECOMBINANT DNA. Molecular Genetics Activity #6 page 1

MOLECULAR GENETICS GENETIC ENGINEERING RECOMBINANT DNA. Molecular Genetics Activity #6 page 1 AP BIOLOGY MOLECULAR GENETICS ACTIVITY #6 NAME DATE HOUR RECOMBINANT DNA GENETIC ENGINEERING Molecular Genetics Activity #6 page 1 GENETIC ENGINEERING Molecular Genetics Activity #6 page 2 PART I: PRODUCING

More information