Antibody Engineering. Methods and Protocols. Benny K. C. Lo
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1 Antibody Engineering Methods and Protocols Benny K. C. Lo
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3 Preface Contributors v x i PART I: ANTIBODY SEQUENCE AND STRUCTUR E 1 Internet Resources for the Antibody Enginee r Benny K. C. Lo and Yu Wai Chen 3 2 The Kabat Database and a Bioinformatics Exampl e George Johnson and Tai Te Wu IMGT, The International ImMunoGeneTics Information System, Marie-Paule Lefranc 27 4 Antibody Variable Regions : Toward a Unified Modeling Method Nicholas Whitelegg and Anthony R. Rees Studying Antibody Conformations by Ultracentrifugatio n and Hydrodynamic Modelin g Stephen E. Harding, Emma Longman, Beatriz Carrasco, Alvaro Ortega, and Jose Garcia de la Torre 93 PART II : ANTIBODY-LEAD GENERATIO N 6 PCR Cloning of Human Immunoglobulin Gene s James D. Marks and Andrew Bradbury Antibody Humanization by CDR Graftin g Benny K. C. Lo Selection of Human Antibodies from Phage Display Librarie s James D. Marks and Andrew Bradbury Production of Human Single-Chain Antibodie s by Ribosome Display Mingyue He, Neil Cooley, Alison Jackson, and Michael J. Taussig Production of Human Antibodies from Transgenic Mic e C. Geoffrey Davis, Xiao-Chi Jia, Xiao Feng, and Mary Haak-Frendscho Selection of Internalizing Antibodies for Drug Delivery James D. Marks 201
4 12 Engineering Multivalent Antibody Fragments for In Vivo Targetin g Anna M. Wu Production of Recombinant Bispecific Antibodie s Roland E. Kontermann, Tina Völkel, and Tina Korn 227 PART III : ANTIBODY EXPRESSION AND OPTIMIZATIO N 14 Expression and Isolation of Recombinant Antibody Fragments in E. coli Keith A. Charlton Expression of Recombinant Antibodies in Mammalian Cell Line s Paul J. Yazaki and Anna M. Wu Human Antibody Production Using Insect-Cell Expression System s Mary C. Guttieri and Mifang Liang Antibody Production in Transgenic Plant s Eva Stoger, Stefan Schillberg, Richard M. Twyman, Rainer Fischer, and Paul Christou Directed Mutagenesis of Antibody Variable Domain s Kevin Brady and Benny K. C. Lo Antibody Affinity Maturation by Chain Shufflin g James D. Marks Antibody Affinity Maturation by Random Mutagenesi s Ikuo Fujii Developing a Minimally Immunogenic Humanized Antibod y by SDR Grafting Syed V. S. Kashmiri, Roberto De Pascalis, and Noreen R. Gonzales 36 1 PART IV : ANTIBODY CHARACTERIZATION AND NOVEL APPLICATION S 22 Antibody Purification by Column Chromatograph y Andrea Murray, C. Rosamund L. Graves, Kevin Brady, and Benny K. C. Lo Affinity Measurement Using Surface Plasmon Resonanc e Robert Karlsson and Anita Larsson Kinetic Exclusion Assays to Study High-Affinity Bindin g Interactions in Homogeneous Solution s Robert C. Blake II and Diane A. Blake Characterization of Antibody-Antigen Interaction s by Fluorescence Spectroscopy Sotiris Missailidis and Kevin Brady 431
5 26 Antibody Epitope Mapping Using Arrays of Synthetic Peptide s Ulrich Reineke Mapping Antibody :Antigen Interactions by Mass Spectrometr y and Bioinformatic s Alexandra Huhalov, Daniel I. R. Spencer, and Kerry A. Chester Radiometal Labeling of Antibodies and Antibody Fragment s for Imaging and Therap y Ilse Novak-Hofer, Robert Waibel, Kurt Zimmermann, Roger Schibli, Jürgen Grünberg, Kerry A. Chester, Andrea Murray, Benny K. C. Lo, Alan C. Perkins, and P. August Schubiger Production and Characterization of Anti-Cocain e Catalytic Antibodie s Paloma de Prada and Donald W. Landry Recombinant Immunotoxins in the Treatment of Cance r Ira Pastan, Richard Beers, and Tapan K. Bera Antibodies in Proteomic s Andrew R. M. Bradbury, Nileena Velappan, Vittorio Verzillo, Milan Ovecka, Roberto Marzari, Daniele Sblattero, Leslie Chasteen, Robert Siegel, and Peter Pavlik Targeting of Antibodies Using Aptamers Sotiris Missailidis 54 7 Index 557
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