Research of Human Microbiome Sequencing and Comparison

Transcription

1 BIOTECHNOLOGY BULLETIN Research of Human Microbiome Sequencing and Comparison Xu Xiaoyu 1 Chen Jinghua 1 Liu He 2 1 School of Medicine and Pharmaceutical Jiangnan University Wuxi School of Environment and Civil Engineering Jiangnan University Wuxi Abstract The human microbiota which reside in or on the human body affect human heath and has closely associated to obesity diabetes coronary heart disease colon carcinoma. In recent years great importance has been attached in the research of human microbiome. The paper reviewed the progress of human microbiome sequencing and comparison. Key words Human microbiome Metagenomics Gut microbiota Oral microbiota % - 2% microbiome metagenome NIH Karen Nelson Human Microbiome Project HMP BE iist@ jiangnan. edu. cn

2 Grice S rrna K B12 Fierera % 13% Paulino 10 PCR MetaHIT Broad range PCR S /ITS Gao 11 16S rdna Elizabeth Kurokawa 15 18

3 68 Biotechnology Bulletin Xu Bacteroides thetaiotaomicron DNA Methanobrevibacter smithii Methanobrevibacter smithii Peer Bork Sanger 17 Eckburg DNA Sanger Li Firmicutes Bacteroidetes Proteobacteria Actinobacteria Verrucomicrobia Fusobacteria Bacteroidetes Firmicutes Methanobrevibacter smithii Gordon 16S S rrna 2008 NIH NIDCR

4 Burton Zaura S rrna 3 42% L. iners Lactobacillus 3 84% Zhou 16S rrna % 65% 3 16S rrna % 94% L. crispatus L. iners % Lactobacillus Atopobium vaginae 15 Lactobacillus Atopobi- Megasphaera Leptotrichia 3 Bik 28 PCR 10 um Lactobacillus T-RFLP gene-based 16S rrna Lazarevic PCR Lactobacillus V5 82 Illumina 135 Firmicutes Proteobacteria Actinobacteria Fusobacteria Bacteroidetes Nasidze 30 16S rrna Lactobacillus Lactobacillus bp 101 Bifidobacterium Gardnerella Atopobium Coryne- 39 bacterium Janthinobacterium8 Lactobacillus Bifidobacterium Gardnerella Gemel % la Prevotella Pseudomonas Streptococcus 34 Oakley % Lactobacillus lactobacilli Atopobium Hyman 28 16S rrna 10 86% Lactobacillus

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