DNA, RNA, Protein Markers & Cloning Vectors DNA, RNA, Protein Markers & Cloning Vectors DNA, RNA, Protein Markers & Cloning Vectors

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1 DNA, RNA, Protein Markers & DNA, RNA, Protein Markers & 218 BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

2 DNA Markers Item No. BBI Range Catalogue DNA, RNA, Protein Markers & B600303(GM303) B600303(GM30) B800305(GM305) B500331(GM331) B500331(GM332) B50035(GM35) B50033(GM33) B600333(GM333) B600333(GM33) B620001(SGM01) 9 DNA fragments: 25, 50, 75, 100, 150, 200, 300, 00, 9 DNA fragments: 25, 50, 75, 100, 150, 200, 300, 00, 10 DNA fragments: 50, 100, 150, 200, 250, 300, 350, 00, 50, 6 DNA fragments: 100, 200, 300, 00, 500, 600 bp 6 DNA fragments: 100, 200, 300, 00, 500, 600 bp 13 DNA fragments: 50, 100X2, 150, 200X2,250, 300, 00, 500X2, 600, 700, 800, 900 and 1031 bp 10 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900 and 1000 bp 6 DNA fragments: 100, 300, 500, 700, 900, 1200 bp 6 DNA fragments: 100, 300, 500, 700, 900, 1200 bp 6 DNA fragments: 100, 250, 500, 750, 1000, 1 B620001(SGM02) B800102(M102O-1) B800102(M102O-2) B810102(M102R-1) B810102(M102R-2) B800107(M107O-1) B800107(M107O-2) B810107(M107R-1) B810107(M107R-2) B800109(M109-A) B800109(M109-B) B600335(GM335) B600335(GM336) B600337(GM337) B600337(GM338) B600339(GM339) B600339(GM30) B60001(GM01) 6 DNA fragments: 100, 250, 500, 750, 1000, 1 9 DNA fragments: 100, 200, 300, 00, 500, 600, 800,1000 and 1 9 DNA fragments: 100, 200, 300, 00, 500, 600, 800,1000 and 1 9 DNA fragments: 100, 200, 300, 00, 500, 600, 800, 1000 and 1 9 DNA fragments: 100, 200, 300, 00, 500, 600, 800, 1000 and 1 11 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900, 1000 and 1 11 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900, 1000 and 1 11 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900, 1000 and 1 11 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900, 1000 and 1 10 DNA fragments:150, 250, 350, 50, 550, 650, 750, 850, 1000, 1 10 DNA fragments:150, 250, 350, 50, 550, 650, 750, 850, 1000, 1 6 DNA fragments: 100, 250, 500, 750, 1000, 2000 bp 6 DNA fragments: 100, 250, 500, 750, 1000, 2000 bp 6 bands: 200, 00, 600, 800, 1000, 1 6 bands: 200, 00, 600, 800, 1000, 1 6 DNA fragments: 200, 00, 700, 1000, 1500, 2000 bp 6 DNA fragments: 200, 00, 700, 1000, 1500, 2000 bp 10 DNA fragments: 200, 00, 600, 800, 1000, 1200, 100, 1600, 1800, 2000 bp B60031(GM31) B60031(GM32) B50037(GM37) B610003(SGM03) B610003(SGM0) B800101(M101O-1) 6 DNA fragments: 300, 500, 1000, 2000, 2 6 DNA fragments: 300, 500, 1000, 2000, 2 1 DNA fragments: 100, 200, 300, 00, 500x2, 600, 700, 800, 900 and 1000x2, 1200, 1500, 2000, 3000 bp 9 DNA fragments: 100, 250, 500,750,1000,1500,2000,3000,5000 bp 9 DNA fragments: 100, 250, 500,750,1000,1500,2000,3000,5000 bp 9 DNA fragments: 0.5kb, 1 kb, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 219

3 DNA, RNA, Protein Markers & B800101(M101O-2) B810101(M101R-1) B810101(M101R-2) B80003(GM03) B800255(E255) B800103(M103O-1) B800103(M103O-2) B810103(M103R-1) 9 DNA fragments: 0.5 kb, 1 kb, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb 9 DNA fragments: 0.5 kb, 1 kb, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb 9 DNA fragments: 0.5 kb, 1 kb, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb 13 DNA fragments: 0.2 kb, 0.5, 1, 1.5, 2, 2.5, 3,, 5, 6, 8, 10, 1 kb 10 DNA fragments: 1,503, 10,086, 15,00, 17,053, 23,99, 2,508, 29,96, 33,98, 38,16 and 8,502 bp 16 DNA fragments: 100, 200, 300, 00,500, 600, 800, 1000, 1500, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb 16 DNA fragments: 100, 200, 300, 00,500, 600, 800, 1000, 1500, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb 16 DNA fragments: 100, 200, 300, 00, 500,600, 800, 1000, 1500, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb B810103(M103R-2) 16 DNA fragments: 100, 200, 300, 00, 500,600, 800, 1000, 1500, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb B800273(E273) 2 DNA fragments: 100, 22, 702, 1,26, 1371,1,503, 1,929, 2,323, 3,675,,32,,827, 5,686, 6,369, 7,639, 8,5, 10,086, 15,00, 23,99, 2,508, 29,96, 33,98, 38,16, and 8,502 bp B80010(M10R-1) 7 DNA fragments : 23130*, 916, 6557, 361*, 2322, 2027 & 56 B80010(M10R-2) 7 DNA fragments : 23130*, 916, 6557, 361*, 2322, 2027 & 56 B800105(M105R-1) B800105(M105R-2) RNA Markers Item No. B800818(DM0818) B801831(BSM1831) B801831(BSM1833) B801821(BSM1821) B801821(BSM1823) Protein Markers Item No. C600201(BM201) C600523(BM523) C60052(BM52) B8001(BSM01) B800661(BSM0661) B80031(BSM031) 10 DNA Fragments 23130*,916, 6557, 361*, 2322, 2027, 1500, 1000, 56, 300 bp 10 DNA Fragments 23130*,916, 6557, 361*, 2322, 2027, 1500, 1000, 56, 300 bp Range 2 bands: 1776, 3566 bp 7 bands: 100, 200, 300, 00, 600, 800, 1000 bp 7 bands: 100, 200, 300, 00, 600, 800, 1000 bp 8 bands: 200, 500, 1000, 1500, 2000, 3000, 000, 6000 bp 8 bands: 200, 500, 1000, 1500, 2000, 3000, 000, 6000 bp Range 9 protein bands:.1, 6.5, 9.5, 1., 20, 27, 35, 5, 66 kda 5 bands: 1., 31, 2.7, 66.2, 97. kda 6 bands: 1., 20, 31, 5, 66.2, 98 kda 6 band: 20, 25, 35, 50, 85, 120 kda 1 band: 10, 15, 20, 25, 30, 0, 50, 60, 70, 85, 100, 120, 150, 200 kda 1 band: 1., 18., 25, 35, 5, 66.2, 116 kda B800671(BSM0671) B800363(BG00363) B80036(BG0036) kda Wide Range Protein Molecular Weight Marker, Prestained kda Wide Range Protein Molecular Weight Marker, Prestained kda Wide Range 3 Color Prestained Protein Ladder 220 BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

4 BBI Phages, Plasmid DNA, & Related Products DNA Phages & Plasmids Catalogue DNA, RNA, Protein Markers & Item No. Range B600011(MDD22) B610010(MDD21) B620003(MSD1) B810015(MSD15) B522219(MSD16) B522201(MSD17) B62033 (BS33) Lambda DNA Lambda DNA (dam-, dcm-) pbr322 DNA puc 18 DNA puc 19 DNA puc 57 DNA pucm-t Cloning Vector B62033 (BS3) B62035(BS35) B62035(BS36) pucm-t Cloning Vector pucm-t Cloning Vector Kit pucm-t Cloning Vector Kit Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 221

5 DNA, RNA, Protein Markers & DNA Markers 25- DNA Marker, Ready-to-Use 9 DNA fragments: 25, 50, 75, 100, 150, 200, 300, 00, The intensity of the 00 bp band is increased to yield an internal reference indicator. Ready-to use. The marker should be loaded on high concentration gels ( % agarose gel or 10% PAGE) to properly view bands of low molecular weight. Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 100 bp band. B600303(GM303) 20 loadings (120 μl) B600303(GM30) 5x20 loadings (5x120 μl) $ bp 300 bp 200 bp 150 bp 100 bp 75 bp 50 bp 25 bp 50- DNA Marker 10 DNA fragments: 50, 100, 150, 200, 250, 300, 350, 00, 50, The marker should be loaded on high concentration gels ( % agarose gel or 10% PAGE) to properly view bands of low molecular weight. Usage: 5 μl /load B800305(GM305) 200 loadings (1 ml) bp Low Range DNA Marker, Ready-to-Use 6 DNA fragments: 100, 200, 300, 00, 500, 600 bp $7.50 The intensity of the 100bp band is increased to yield an internal reference indicator. Ready-to use. The marker should be loaded on high concentration gels ( % agarose gel or 10% PAGE) to properly view bands of low molecular weight. Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 100 bp band. B500331(GM331) 50 loadings (300 μl) B500331(GM332) 5x50 loadings (5x300 μl) $ bp DNA Marker, Ready-to Use 13 DNA fragments: 50, 100X2, 150, 200X2,250, 300, 00, 500X2, 600, 700, 800, 900 and 1031 bp The intensity of the 100, 200, and bands are increased to yield internal reference indicators. Storage Buffer: 1XTE (10 mm Tris-HCl, ph8.0, 1.0 mm EDTA) 6x Loading Buffer: 30% glycerol, 0.12% bromophenol blue, 0.12% xylene cyanol Usage: 6 μl /load B50035(GM35) 50 loadings (300 μl) $ bp DNA Marker, Ready-to Use 10 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900 and 1000 bp The intensity of the band is increased to yield an internal reference indicator Concentration: 50 ng/5 μl Storage Buffer: 1XTE (10 mm Tris-HCl, ph8.0, 1.0 mm EDTA) 6x Loading Buffer: 30% Glycerol, 0.12% Bromophenol blue, 0.12% Xylene cyanol Usage: 6 μl /load B50033(GM33) 50 loadings (300 μl) $ bp 00 bp 300 bp bp % agarose 1000 bp x bp 100 bp 222 BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

6 BBI Catalogue DNA, RNA, Protein Markers & bp DNA Marker, Ready-to-Use 6 DNA fragments: 100, 300, 500, 700, 900, 1200 bp The intensity of the 700 bp band is increased to yield an internal reference indicator Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 700 bp band. B600333(GM333) 50 loadings (300 μl) B600333(GM33) 5x50 loadings (5x300 μl) $ bp 900 bp 700 bp 300 bp 100 bp DNA Marker, Ready-to-Use 6 DNA fragments: 100, 250, 500, 750, 1000, 1 The intensity of the 700 bp band is increased to yield an internal reference indicator Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 700 bp band. B620001(SGM01) 50 loadings (300 μl) B620001(SGM02) 5x50 loadings (5x300 μl) $ bp 750 bp 250 bp 100 bp DNA Marker, Original Form 9 DNA fragments: 100, 200, 300, 00, 500, 600, 800,1000 and 1 Supplied with 6x loading buffer Concentration: 50 ng/ 5 μl Recommended loading volume: 5 μl Storage Buffer: 1 XTE (10 mm Tris-HCl, ph 8.0, 1.0 mm EDTA) 6x Loading Buffer: 30% Glycerol, 0.12% Bromophenol blue, 0.12% Xylene cyanol B800102(M102O-1) 100 loadings (500 μl) $6.00 6X Loading Buffer 200μ l per pack Supplied with B800102(M102O-1) B800102(M102O-2) 5x100 loadings (5x500 μl) $ X Loading Buffer 5X 200 μ l per pack Supplied with B800102(M102O-2) DNA Marker, Ready to Use 9 DNA fragments: 100, 200, 300, 00, 500, 600, 800, 1000 and 1 Supplied with 6x loading buffer Concentration: 50 ng/ 6μl Recommended loading volume: 50 ng/ 6μl Storage Buffer: 10 mm Tris-HCl, ph 8.0, 1 mm EDTA, and 1 X Loading Buffer ( 5% Glycerol, 0.02% Bromophenol blue, 0.02% Xylene cyanol) B810102(M102R-1) 100 loadings (600 μl) $6.00 B810102(M102R-2) 5x100 loadings (5x600 μl) $ Quantity ng 1000 bp 100 ng 800 bp 80 ng 600 bp 60 ng 50 ng 00 bp 0 ng 300 bp 30 ng 200 bp 20 ng 100 bp 10 ng Quantity ng 1000 bp 100 ng 800 bp 80 ng 600 bp 60 ng 50 ng 00 bp 0 ng 300 bp 30 ng 200 bp 20 ng 100 bp 10 ng Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 223

7 DNA, RNA, Protein Markers & DNA Marker, Original Form 11 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900, 1000 and 1 Supplied with 6X Loading Buffer The DNA 100 bp Marker consists of 11 fragments ranging from base pair (BP). The intensity of band has been increased to serve as a reference. This 100 bp Marker is specially prepared for visualizing small DNA fragments on the gel. Concentration: 500 ng / 5 μl Recommended loading volume: 5 μl Storage Buffer: 1 X TE and 1X loading buffer ( 5% Glycerol, 0.02% Bromophenol blue, 0.02% Xylene cyanol) B800107(M107O-1) 100 loadings (500 μl) $8.00 6X Loading Buffer 200 μ l per pack Supplied with B800107(M107O-1) B800107(M107O-2) 5x100 loadings (5x500 μl) $ X Loading Buffer 5X 200 μ l per pack Supplied with B800107(M107O-2) DNA Marker, Ready-to Use 11 DNA fragments: 100, 200, 300, 00, 500, 600, 700, 800, 900, 1000 and 1 Supplied with 6X Loading Buffer The DNA 100 bp Marker consists of 11 fragments ranging from The intensity of the band has been increased to serve as an internal reference. The marker is specially prepared for visualizing small DNA fragments on gels. Concentration: 500 ng / 6 μl Recommended loading volume: 6 μl Storage Buffer: 1 X TE and 1X loading buffer ( 5% Glycerol, 0.02% Bromophenol blue, 0.02% Xylene cyanol) bp DNA Marker, Ready-to-use 10 DNA fragments:150, 250, 350, 50, 550, 650, 750, 850, 1000, 1 Supplied with 6X Loading Buffer Concentration: 500 ng / 6 μl Recommended loading volume: 6 μl Storage Buffer: 1 X TE and 1X loading buffer ( 5% Glycerol, 0.02% Bromophenol blue, 0.02% Xylene cyanol) B800109(M109-A) B800109(M109-B) 100 loading (600 µl) bp DNA Marker, Ready-to-Use 5x100 loadings (5x600 µl) 6 DNA fragments: 100, 250, 500, 750, 1000, 2000 bp The intensity of the 750bp band is increased to yield an internal reference indicator $8.00 $ Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 750 bp band. B600335(GM335) 50 loadings (300 μ l) B600335(GM336) 5x50 loadings (5x300 μ l) $ bp 900 bp 800 bp 700 bp 600 bp 00 bp 300 bp 200 bp 100 bp bp 900 bp 800 bp 700 bp 600 bp 00 bp 300 bp 200 bp 100 bp bp 1000 bp 750 bp 250 bp 100 bp 22 BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

8 BBI Catalogue DNA, RNA, Protein Markers & DNA Marker, Ready-to-Use 6 bands: 200, 00, 600, 800, 1000, 1 The intensity of the 1000bp band is increased to yield an internal reference indicator Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 1000bp band. B600337(GM337) 50 loadings (300 μl) B600337(GM338) 5x50 loadings (5x300 μl) $ bp 800 bp 600 bp 00 bp 200 bp bp DNA Marker, Ready-to-Use 6 DNA fragments: 200, 00, 700, 1000, 1500, 2000 bp The intensity of the 1000bp band is increased to yield an internal reference indicator Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 1000 bp band. B600339(GM339) 50 loadings (300 μl) B600339(GM30) 5x50 loadings (5x300 μl) $ bp DNA Marker Plus, Ready-to-Use 10 DNA fragments: 200, 00, 600, 800, 1000, 1200, 100, 1600, 1800, 2000 bp The intensity of the 1000bp band is increased to yield an internal reference indicator. Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 1000 bp band. B60001(GM01) 50 loadings (300 μl) $ DNA Marker, Ready-to-Use 6 DNA fragments: 300, 500, 1000, 2000, 2 The intensity of the 150bp band is increased to yield an internal reference indicator. Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 1 band. B60031(GM31) 50 loadings (300 μl) B60031(GM32) 5x50 loadings (5x300 μl) $ bp Marker, Ready-to Use 1 DNA fragments: 100, 200, 300, 00, 500x2, 600, 700, 800, 900 and 1000x2, 1200, 1500, 2000, 3000 bp The intensities of the 500 and 1000bp bands are increased to yield internal reference indicators. Concentration: 200 μg/ml Recommended loading volume: 6 μl Storage Buffer: 1XTE (10 mm Tris-HCl, ph 8.0, 1.0 mm EDTA) 6x Loading Buffer: 30% Glycerol, 0.12% Bromophenol blue, 0.12% Xylene cyanol B50037(GM37) 50 loadings (300 μl) $ bp 1500bp 1000bp 2000bp bp 00bp 200bp 2500bp 2000bp 1500bp 1000bp 500bp 300bp bp Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 225

9 DNA, RNA, Protein Markers & bp DNA Marker Plus, Ready-to-Use 9 DNA fragments: 100, 250, 500, 750, 1000, 1500, 2000, 3000, 5000bp The intensity of the 750 bp band is increased to yield an internal reference indicator. Usage: 6 μl /load containing 75 ng of each DNA fragments and 150 ng of 750 bp band. B610003(SGM03) 50 loadings (300 μ l) B610003(SGM0) 5x50 loadings (5x300 μ l) $ bp 3000 bp 2000 bp bp 750 bp 250 bp 100 bp bp DNA Marker, Original Form 9 DNA fragments: 0.5kb, 1 kb, 2kb, 3kb, kb, 5kb, 6kb, 8kb, and 10 kb DNA KB Marker consists of 9 DNA fragments ranging from kb. The intensity of the 3 kb band has been increased to yield an internal reference indicator. Concentration: 250 ng / 5 μl Recommended loading volume: 5 μl Storage Buffer: 1XTE (10 mm Tris-Hcl, ph 8.0, 1 mm EDTA) 6x Loading Buffer: 30% Glycerol, 0.12% Bromophenol blue, 0.12% Xylene cyanol B800101(M101O-1) 100 loadings (500 μl) $8.00 6X Loading Buffer 200 μ l per pack Supplied with B800101(M101O-1) B800101(M101O-2) 5X100 loadings (5X500 μl) $ X Loading Buffer 5X 200 μ l per pack Supplied with B800101(M101O-2) bp DNA Marker, Ready To Use 9 DNA fragments: 0.5kb, 1 kb, 2 kb, 3 kb, kb, 5 kb, 6kb, 8kb, and 10 kb DNA KB Marker consists of 9 DNA fragments ranging from kb. The intensity of the 3 kb band has been increased to yield an internal reference indicator. Concentration: 250 ng / 6 μl Recommended loading volume: 6 μl Storage Buffer: 1 X TE and 1X loading buffer (5% Glycerol, 0.02% Bromophenol blue, 0.02% Xylene cyanol) B810101(M101R-1) 100 loadings (600 μ l) $8.00 B810101(M101R-2) 5x100 loadings (5x600 μ l) $ kb to 1 kb DNA Marker 13 DNA fragments: 0.2 kb, 0.5, 1, 1.5, 2, 2.5, 3,, 5, 6, 8, 10, 1 kb This marker is suited for evaluating large fragments >10 kb in length. A combination of several single-cut λ digests, the marker possesses fragments with sizes ranging from 0.2 to 1 kb. The High-Range Marker was separated on a 0.35% agarose gel and stained with SYBR Green I Nucleic Acid Stain. For ideal separation, pulsed-field gel electrophoresis is recommended. B80003(GM03) 100 loadings (500 μ l) $ kp 8 kp 6 kp 5kp kp 3kp 2kp 1kp 0.5kp 10 kp 8 kp 6 kp 5kp kp 3kp 2kp 1kp 0.5kp 226 BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

10 BBI Catalogue DNA, RNA, Protein Markers & 1.5 kb to 8.5 kb High Range DNA Marker 10 DNA fragments: 1,503, 10,086, 15,00, 17,053, 23,99, 2,508, 29,96, 33,98, 38,16 and 8,502 bp This marker is suited for evaluating large fragments >10 kb in length. A combination of several single-cut λ digests, the marker possesses fragments ranging in size from 1.5 to 8.5 kb. High-Range Marker was separated on a 0.35% agarose gel and stained with SYBR Green I Nucleic Acid Stain. For ideal separation, pulsed-field gel electrophoresis is recommended. B800255(E255) 150 μg $ ,502 bp 38,16 33,98 29,96 2,508/23,99 17,053/15,00 10,086 1,503 High Range 100 bp-10 Kb Wide Range DNA Logical Marker, Original Form 16 DNA fragments: 100, 200, 300, 00,500, 600, 800, 1000, 1500, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb The DNA Logic-Marker consists of 16 DNA fragments ranging from kb. Each band contains a Logical amount of DNA that correlates to its size. Each marker reveals a unique pattern for both the recognition of bands on a gel, and also gives a quantitative gradient of DNA concentration. Thus, both the size and the quantity of a particular DNA fragment can be achieved at a glance. Concentration: 920 ng / 5μl Recommended loading volume: 5 μl Storage Buffer: 1 TE (10 mm Tris-HCl, ph 8.0, 1 mm EDTA) 6x Loading Buffer: 30% Glycerol, 0.12% Bromophenol blue, 0.12% Xylene cyanol B800103(M103O-1) 100 loadings (500 μl) $ X Loading Buffer 200 μ l per pack Supplied with B800103(M103O-1) B800103(M103O-2) 5x100 loadings (5X500 μl) $ X Loading Buffer 5X 200 μ l per pack Supplied with B800103(M103O-2) 100 bp-10 Kb Wide Range DNA Logical Marker, Ready to use 16 DNA fragments: 100, 200, 300, 00, 500,600, 800, 1000, 1500, 2 kb, 3 kb, kb, 5 kb, 6 kb, 8 kb, and 10 kb The DNA Logic-Marker consists of 16 DNA fragments ranging from kb. The DNA Logic-Marker was formulated to yield such a pattern that each band contains a Logical amount of DNA fragment, correlating to its size. The Logic-Marker not only reveals a unique pattern for easy recognition of the bands on the gel, but also gives a quantitative gradient of the DNA amount. Thus, both the size and the quantity of a particular DNA fragment can be achieved at a glance. Concentration: 920 ng / 6 μl Recommended loading volume: 6 μl Storage Buffer:1xTE and 1x loading buffer (5% Glycerol, 0.02% Bromophenol blue, 0.02% Xylene cyanol) B810103(M103R-1) 100 loadings (600 μ l) $80.00 B810103(M103R-2) 5x100 loadings (5x600 μ l) $ bp-8kb Wide Range DNA Marker 2 DNA fragments: 100, 22, 702, 1,26, 1371,1,503, 1,929, 2,323, 3,675,,32,,827, 5,686, 6,369, 7,639, 8,5, 10,086, 15,00, 23,99, 2,508, 29,96, 33,98, 38,16, and 8,502 bp A combination of the Low-Range Marker I and the High-Range Marker. Possessing 2 fragments ranging from 0.1 to 8.5 kb in length, the marker provides an excellent assortment of fragment sizes suitable for many applications. size Quantly 10 kb 100 ng 8 kb 80 ng 6 kb 60 ng 5 kb 50 ng kb 0 ng 3 kb 30 ng 2 kb 20 ng ng 1000 bp 100 ng 800 bp 80 ng 600 bp 60 ng 50 ng 00 bp 0 ng 300 bp 30 ng 200 bp 20 ng 100 bp 10 ng 1.7% Agarose Gel size Quantly 10 kb 100 ng 8 kb 80 ng 6 kb 60 ng 5 kb 50 ng kb 0 ng 3 kb 30 ng 2 kb 20 ng ng 1000 bp 100 ng 800 bp 80 ng 600 bp 60 ng 50 ng 00 bp 0 ng 300 bp 30 ng 200 bp 20 ng 100 bp 10 ng bp / B800273(E273) 200 μg $ % agarose Wide-ramge Marker Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 227

11 DNA, RNA, Protein Markers & Lambda DNA / HindIII Marker, Ready to use 7 DNA fragments : 23130*, 916, 6557, 361*, 2322, 2027 & 56 Lambda DNA is completely digested by HindIII, phenol extracted, ethanol precipitated and dissolved in 10 mm Tris- HCl (ph 7.6) and 1 mm EDTA. HindIII digest of DNA yields 7 DNA fragments. Concentration: 550 ng / 6μl Recommend loading volume: 6 μl Storage Buffer:10 mm Tris-HCl (ph 8.0) and 1 mm EDTA, 0.02%, bromophenol blue, 0.02% Xylene cyanol and 5% glycerol *Note: the cohesive ends (12b cos site of bacteriophage λ) of the bp and 361 bp fragments may anneal to form a large band. These fragments (marked by *) can be separated by heating to 65 for 5 min and then cooling on ice for 3 min. B80010(M10R-1) 100 loadings (600 μ l) $18.00 B80010(M10R-2) 5X100 loadings (5X600 μ l) $86.00 Lambda DNA / HindIII Plus, Ready to use 10 DNA Fragments 23130*,916, 6557, 361*, 2322, 2027, 1500, 1000, 56, 300 bp Hind III-digested Lambda DNA possesses a big gap between the 56 bp and 2027 bp bands. The Lambda DNA/Hind III Plus marker was developed to fill this gap. Three unique bands (0.3, 1, 1.5kb) have been added to the original marker, providing better visualization in this range. Concentration: 550 ng / 6μl Recommended loading volume: 6 μl Storage Buffer:10 mm Tris-HCl (ph 8.0) and 1 mm EDTA, 0.02%,Bromophenol blue, 0.02% Xylene cyanol and 5% glycerol *Note: the cohesive ends (12 bp cos site of bacteriophage λ) of the bp and 361 bp fragments may anneal to form a large band. These fragments (marked by *) can be separated by heating to 65 for 5 min and then cooling on ice for 3 min. B800105(M105R-1) 100 loadings (600 μl) $18.00 B800105(M105R-2) 5X100 loadings (5X600 μl) $75.00 RNA Markers 16S + 23S RNA Marker Lyophilized 500 applications Source: E. coli Ribosomal RNA Comprised of 2 bands: 1,776 bp and 3,566 bp RNase: None Detected Storage: -70 C B800818(DM0818) 2.5 mg $ % agarose Lambda DNA/Hindlll Plus RNA Marker 16S + 23S ,130bp BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

12 BBI Catalogue DNA, RNA, Protein Markers & bp Low Range RNA Molecular Weight Marker 7 bands: 100, 200, 300, 00, 600, 800, 1000 bp The Low Range RNA Molecular Weight Marker is a mixture of 7 chromatographically-purified single-stranded RNA transcripts ( bp). The transcripts are produced from specific templates containing both a fragment of the ptz19r polylinker and lambda phage fragments. Quantity: tested in gel electrophoresis. RNA concentration is determined spectrophotometrically. The absence of ribonucleases is confirmed by appropriate tests. Usage: 2 μl per loading bases B801831(BSM1831) 50 loadings (100 μl) $ B801831(BSM1833) 100 loadings (200 μl) $ % agarose bp High Range RNA Molecular Weight Marker 8 bands: 200, 500, 1000, 1500, 2000, 3000, 000, 6000 bp The High Range RNA Molecular Weight Marker is a mixture of 8 chromatographically-purified single-stranded RNA transcripts ( bp) produced from specific templates that contain a fragment of the ptz19r polylinker and lambda phage fragments. Quantity: tested in gel electrophoresis. RNA concentration is determined spectrophotometrically. The absence of ribonucleases is confirmed by appropriate tests. Usage: 2 μl per loading B801821(BSM1821) 50 loadings (100 μl) $ B801821(BSM1823) 100 loadings (200 μl) $ PROTEIN MARKERS.1-66 kda Low Range Protein Molecular Weight Marker, Ready to use 9 protein bands:.1, 6.5, 9.5, 1., 20, 27, 35, 5, 66 kda This marker is a mixture of purified proteins with known molecular weights. The marker resolves to 6 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coommassie Blue R-250. Quantity: sufficient for 10 & 30 loadings mini-gels (8 10 cm) Usage: 3 μl per loading C600201(BM201) 10 loadings (30 μ l) $15.00 C600201(BM201) 30 loadings (100 μ l) $ % raitive Topvision TM LE GO Agarose (#R091) bases 2μl of SM 821/lane, 8cm length gel, 1 X TAE, 2Wcm ng/2μl kDa Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 229

13 DNA, RNA, Protein Markers & kda Mid Range Protein Molecular Weight Marker, Ready to use 5 bands: 1., 31, 2.7, 66.2, 97. kda The Marker can be applied directly onto SDS-PAGE gel. The marker resolves to 5 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coommassie Blue R-250. Quantity: sufficient for 20 loadings mini-gels (8 10 cm) Usage: 10 μl per loading C600523(BM523) 20 loadings (200 μ l) $ kDa kda Mid Range Protein Molecular Weight Marker, Ready to use 6 bands: 1., 20, 31, 5, 66.2, 98 kda The marker is a mixture of purified proteins with known molecular weights. The marker resolves to 6 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coomassie Blue R-250. Quantity: sufficient for up to 50 loadings mini-gels (8 10 cm) Usage: 10 μl per loading C60052(BM52) 20 loadings (200 μ l) $ kda Mid Range Protein Molecular Weight Marker 6 band: 20, 25, 35, 50, 85, 120 kda Prestained Protein Molecular Weight Marker is a mixture of purified proteins covalently coupled to a blue chromophore. It consists of 6 proteins ranging in apparent molecular weight from approximately 20 kda to 120 kda. The protein concentrations are optimized to yield 6 well-defined blue bands after SDS-polyacrylamide gel electrophoresis. Lot-to-lot variation of the apparent molecular weight of prestained proteins is ~5%. The Marker is supplied in gel loading buffer and is ready-to-use (reconstituted). B8001(BSM01) 2x250 μl $ kda Wide Range Protein Molecular Weight Marker Unstained 1 band: 10, 15, 20, 25, 30, 0, 50, 60, 70, 85, 100, 120, 150, 200 kda BBI Wide Range Protein Molecular Weight Unstained Marker is designed for accurate sizing of proteins in SDSpolyacrylamide gel electrophoresis, as well as on PVDF, nylon and nitrocellulose membranes. BBI Wide Range Protein Molecular Weight Unstained Marker is a mixture of 1 recombinant, highly purified, unstained proteins ranging in size from 10 kda to 200 kda. Quantity: sufficient for up to 100 loadings mini-gels (8 10 cm) Usage: 5 μl per loading for mini-gel / 10 μl per loading for large gel B800661(BSM0661) 2x250 μl $ % Tris-glycine SDS-PAGE Gel kda k 66.2k 5k 31k 20k 1.k Prestained Protein Molecular Weight Marker Apparent NW, kda Protein Source Gel ~120 β-galactosidase E.coli ~85 Bovine serum bovine plasma albumin ~50 ~35 ~25 Ovalbumin Carbonic anhydrase β-lactoglobulin ~20 Lysozyme chicken egg white bovine erythrocytes bovine milk chicken egg white 8-16% Tris-glycine SDS-PAGE Staining with PageBlue TM Protein Staining Solution (#R0571) 230 BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

14 BBI Catalogue DNA, RNA, Protein Markers & kda Wide Range Protein Molecular Weight Marker, Unstained 7 band: 1., 18., 25, 35, 5, 66.2, 116 kda Unstained Protein Molecular Weight Marker is designed for accurate sizing of proteins in SDS-polyacrylamide gel electrophoresis, as well as on PVDF, nylon and nitrocellulose membranes. It is a mixture of 7 native proteins ranging in size from 1. kda to 116 kda. Quantity: sufficient for up to 00 loadings mini-gels (8 10 cm) Usage: 5 μl per loading for mini-gel / 10 μl per loading for large gel 116.0kDa 66.2kDa 5.0kDa 35.0kDa 25.0kDa B80031(BSM031) 2x1 ml $ kDa 1.kDa kda Wide Range Protein Molecular Weight Marker, Prestained 10 band: 10, 15, 25, 35, 0, 55, 70, 100, 130, 170 kda BBI Wide Range Protein Molecular Weight Prestained Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and for approximate sizing of proteins. It is composed of prestained recombinant prokaryotic proteins. BBI Wide Range Protein Molecular Prestained Weight Marker is a 3-color ladder with 10 proteins covering a molecular weight range from 10 to 170 kda. The ladder contains one orange reference band at 70 kda and one green band at 10 kda. Quantity: sufficient for up to 100 loadings mini-gels (8 10 cm) Usage: 5 μl per loading for mini-gel / 10 μl per loading for large gel B800671(BSM0671) 2x250 μl $ kda Wide Range Protein Molecular Weight Marker, Prestained 11 band: 10, 15, 25, 35, 0, 55, 60, 70, 90, 120, 175 kda B800363(BG00363) Wide Range Protein Marker/Ladder consists of 11 proteins that resolve into sharp, tight bands in the range of kda. This unique protein ladder allows you to monitor protein separation during electrophoresis, estimate molecular weight of the protein of interest, and evaluate western blot transfer efficiency. It also includes ~10, ~0 and ~90 kda reference bands coupled with blue dye that allow easy tracking and identification. Quantity: sufficient for up to 100 loadings mini-gels (8 10 cm) Usage: 5 μl per loading for mini-gel / 10 μl per loading for large gel B800363(BG00363) 500 μl $ Gel Tris-Glycine 15% kda ~170 ~130 ~100 ~70 ~50 ~0 ~35 ~25 ~15 ~10 ~175 ~165 ~130 ~115 ~95 ~80 ~70 ~62 ~62 ~51 ~52 ~2 ~29 ~22 ~1 ~10.5 ~2 ~32 ~2 ~17 ~13 ~7 Blot 8-16% Tris-glycine SDS-PAGE kda kda kda Bis-Tris ~12% MOPS buffer ~155 ~120 ~82 ~62 ~55 ~5 ~3 ~26 ~16 ~12 ~5 Bis-Tris ~12% MES buffer Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 231

15 DNA, RNA, Protein Markers & 10-20kDa Wide Range Protein Marker, 3-Color Prestained 12 band: 10, 15, 20, 25, 35, 0, 60, 70, 100, 130, 180, 20kDa The kda Wide Range 3 Color Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 25 kda. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kda and 75 kda respectively) when separated on SDS-PAGE (Tris-glycine buffer).the kda Wide Range 3 Color Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Quantity: sufficient for up to 100 loadings mini-gels (8 10 cm) Usage: 5 μl per loading for mini-gel / 10 μl per loading for large gel kda kda kda ~25 ~235 ~180 ~170 ~130 ~135 ~93 ~100 ~70 ~75 ~53 ~63 ~1 ~8 ~35 ~25 ~20 ~17 ~11 ~30 ~22 ~18 ~1 ~9 ~20 ~165 ~125 ~93 ~72 ~57 ~2 ~31 ~2 ~18 ~15 ~8 Tris-Glycine ~20% Bis-Tris 10% MOPS buffer Bis-Tris 10% MES buffer B80036(BG0036) 500 μl $ Phages, Plasmid DNA, & Related Products DNA Phages & Plasmids Item No. Description B600011(MDD22) B610010(MDD21) B620003(MSD1) B810015(MSD15) Lambda DNA Used as one of the substrates in restriction enzyme research and for the analysis of restriction enzyme activity. Phage is isolated from the heat inducible lysogen E.coli W3350 (ci857 Sam7). DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mm Tris-HCl (ph 7.6) and 1 mm EDTA. It contains 8502 bases pairs. Concentration: 0.3 mg/ml. Storage Buffer: 10 mm Tris-HCl (ph 7.8) and 1 mm 0.1 mg $63.00 Lambda DNA (dam -, dcm - ) 0.1 mg $63.00 Used as one of the substrates in restriction enzyme research and for the analysis of endonuclease sensitivity to Dam or Dcm methylation activity. Phage is isolated from heat inducible lysogen E.coli GM2163 (ci857 Sam7). DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mm Tris-HCl (ph 7.8) and 1 mm EDTA. It contains 8502 bases pairs. Concentration: 0.3 mg/ml Storage Buffer: 10 mm Tris-HCl (ph 7.8) and 1 mm EDTA. pbr322 DNA 50 μ Isolated from E.coli (dam +, dcm + ) and purified by ultra centrifugation. The molecule is a doublestranded circle (361 bp in length). Concentration: 0.25mg/ml Storage Buffer: 10 mm Tris-HCl (ph 7.8) and 1 mm EDTA puc18 DNA 50 μ puc18 DNA is isolated from E.coli strain DH5α. Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double-stranded, 2686 bases pairs in length. Concentration: mg/ml Storage Buffer: 10 mm Tris-HCl (ph 7.8) and 1 mm EDTA 232 BIO BASIC INC. Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA

16 BBI Catalogue DNA, RNA, Protein Markers & B522219(MSD16) B522201(MSD17) puc19 DNA Isolated from E.coli strain DH5α. Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double-stranded, 2686 bases pairs in length. Concentration: 0.2 mg/ml Storage Buffer: 10mM Tris-HCl (ph7.8) and 1 mm EDTA puc57 DNA Isolated from E.coli strain DH5. Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double-stranded, 2710 bases pairs in length. Concentration: mg/ml Storage Buffer: 10 mm Tris-HCl (ph 7.8) and 1 mm EDTA 50 μ 50 μ pucm-t Cloning Vector Designed for the cloning of PCR products possessing overhanging 3 A-residues produced by Taq or other DNA polymerase. The DNA sequence of the pucm-t vector is the same as puc19 (GenBank Accession Number M77789) except for minor differences at the multiple cloning site. The vector can also be used for the cloning of blunt-ended DNA fragments, which requires the addition of an A- residue at the 3 end prior to cloning (the A-Tailing Kit (B620513(BS51)) is suitable here). The procedure is simple & fast. 1 μg of the pucm-t Cloning Vector is used for 20 reactions. Store at - 20 C Item No. Description B62033(BS33) B62033(BS3) B62035(BS35) B62035(BS36) pucm-t-cloning Vector pucm-t-cloning Vector pucm-t-cloning Vector Kit (with 100 U T DNA Ligase, 50 μl 10x Ligation Buffer, 50 μl 50% PEG 000, 1 ml ddh 2 O) pucm-t-cloning Vector Kit (with 500 U T DNA Ligase, 250 μl 10x Ligation Buffer, 250 μl 50% PEG 000, 5 ml ddh 2 O) 1 μg 5 μg 20 preps 100 preps $30.00 $ $50.00 $ Add: 20 Konrad Crescent, Markham, ON, L3R 8T Canada Tel:(905) 7 93, (800) Fax:(905) Bailey Avenue, Amherst, New York, 1226 USA 233