Cellular Pathology Techniques Educational Workshops

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1 Cellular Pathology Techniques Educational Workshops UK NEQAS for Cellular Pathology Technique Providing worldwide external quality assessment and proficiency testing for all aspects of tissue diagnostics UK NEQAS for Cellular Pathology Technique The Pathology Centre, Queen Elizabeth Hospital, Sheriff Hill, Gateshead, Tyne and Wear NE9 6SX Tel: +44 (0)

2 Worldwide Participation in External Quality Assessment and Proficiency Testing. UK National External Quality Assessment Service for Cellular Pathology Techniques (UK NEQAS for CPT) is an international not for profit organization providing a comprehensive range of accredited external quality assessment and proficiency testing programmes for all aspects of tissue diagnostics Tissue diagnostics is an extremely important element in the management of patients and therefore the role of external quality assessment is essential in order to provide assurance that a high standard of testing is achieved for all. Errors with tests can have catastrophic results so it is important for UK NEQAS for CPT to ensure early detection of problems and provide education, help and support to organisations Specialist Schemes and education are offered by UK NEQAS for CPT in the following; General Pathology (Routine Histopathology) Neuropathology Renal Biopsy Pathology Muscle Histochemistry Non Gynae Diagnostic Cytology Supplementary Specialisms Education is important to provide useful information that allow for quality improvement in Cellular Pathology Techniques. UK NEQAS for CPT provides a large educational component including; an educational website, best methods and image galleries, annual participant feedback meetings, scientific meetings and seminars, newsletters and educational workshops 2 P a g e

3 Special Staining Beginners / Refresher Theory & Practice June 2016 book now This introductory workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which boasts a purpose built cellular pathology laboratory as part of their state of the art facilities for students and workshop delegates. The workshop will provide a basic introduction and training in the theory and practice in the use of tinctorial stains used in cellular pathology laboratories, through the use of presentations, discussion and laboratory based practical sessions. On completion of the workshop students will have a basic knowledge of Theory of haematoxylin and eosin staining Assessment of staining quality Formalin fixation The use of control sections Classification of dyes Tissue artefacts Recognition of special stains The rationale of special staining techniques and their use in diagnostic histopathology On completion of the workshop students will be able to Recognise a well stained H&E Identify and assess special stains Recognise section artefacts Understand how tissue artefacts are caused and how they may be eliminated or minimised Have an understanding of the classification and molecular structure of dyes Have an understanding of the rationale of dye staining mechanisms and associated problems Assess the quality of their own depts. stained preparations using UK NEQAS for CPT assessment criteria Assess adequacy of formalin fixation Is this workshop the right one for me? This workshop is suitable for anyone who has a basic science background and wishes to gain an understanding of theory and practice in cellular pathology techniques. The workshop will provide the knowledge for participants to recognise, assess and trouble-shoot both haematoxylin and eosin stained sections together with the special staining techniques used in diagnostic cellular pathology. Refreshments and Lunch will be provided. This course is accredited by the Institute of Biomedical Science 3 P a g e

4 Specialist Demonstration Techniques A Theory & Practice June 2016 book now This specialist workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which boasts a purpose built cellular pathology laboratory as part of their state of the art facilities for students and workshop delegates. The workshop is based on the competencies required of the IBMS Cellular Pathology Specialist Portfolio sections 7.2 a, b, c and d. The workshop teaches an in depth knowledge of H&E staining and extracellular proteins such as fibrin and amyloid and the application and principles of carbohydrate and mucin classification and demonstration, through the use of presentations, discussion and laboratory based practical sessions. On completion of the workshop students will have a basic knowledge of Haematoxylin and eosin staining Mordants Fibrin and its demonstration Classification of amyloid Principles and practice of routine staining methods for the demonstration of fibrin and amyloid Pathophysiology of fibrin and amyloid EQA of fibrin and amyloid Classification of carbohydrates Principles and practice of routine staining and impregnation methods for carbohydrate demonstration Principles of the pathophysiology of carbohydrate formation & pathological significance of EQA assessment of stained sections 4 P a g e

5 On completion of the workshop students will be able to Use haematoxylin for various demonstration procedures Understand the advantages, disadvantages and use of haematoxylins made with different mordants Understand the process of progressive and regressive staining Understand the principles of haematoxylin and eosin staining and its value as a primary diagnostic stain Understand how to monitor the quality of H&E staining using standard criteria Assess stained sections for quality Be able to demonstrate extracellular proteins in tissue sections Understand the principles and practice of routine staining methods for extracellular protein demonstration Know the pathological significance of amyloid and fibrin and the techniques used in their demonstration Be able to demonstrate a range of carbohydrates in tissue sections Identify stained carbohydrate present in different tissues Is this workshop the right one for me? This workshop is suitable for those students and laboratory personnel completing the IBMS Specialist Portfolio in Cellular Pathology. The workshop will provide the knowledge for participants to recognise, assess and trouble-shoot both haematoxylin and eosin stained sections together with the special staining techniques used in diagnostic cellular pathology for extracellular proteins such as fibrin and amyloid, as well as the application and principles of carbohydrate and lipid classification and demonstration. Refreshments and Lunch will be provided. This course is accredited by the Institute of Biomedical Science 5 P a g e

6 Specialist Demonstration Techniques B Theory & Practice August 2016 book now This specialist workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which boasts a purpose built cellular pathology laboratory as part of their state of the art facilities for students and workshop delegates. The workshop is based on the competencies required of the IBMS Cellular Pathology Specialist Portfolio sections 7.2 e, f and g. The workshop teaches an in depth knowledge of the application and principles of lipid classification and demonstration, the types and demonstration of common infective agents, and pigments and minerals, through the use of presentations, discussion and laboratory based practical sessions. On completion of the workshop students will have a basic knowledge of Principles and practice of routine staining methods for the demonstration of Lipids, Infective agents, pigments and minerals Lipid storage diseases Types of infective agents most commonly seen and the pathological significance of infective agent demonstration The principles of the pathophysiology of pigment and mineral formation and the types of pigment and mineral normally deposited in tissue How to review cases and material microscopically for internal and external quality control 6 P a g e

7 On completion of the workshop students will be able to Demonstrate lipid in sections Understand the principles and practice of routine staining and histochemical methods for the demonstration of different lipids Know how to review cases and material microscopically for internal and external quality control Be able to demonstrate common infective reagents in tissue sections Know the principles and practice of routine staining and impregnation methods for the demonstration of infective agents Be able to demonstrate pigments and minerals in tissue sections Know the principles and practice of direct visualisation, routine staining and impregnation methods for the demonstration and identification of pigments and minerals Is this workshop the right one for me? This workshop is suitable for those students and laboratory personnel completing the IBMS Specialist Portfolio in Cellular Pathology. The workshop will provide the knowledge for participants to recognise, demonstrate and review special stained sections for special staining techniques used in diagnostic cellular pathology, including lipid expression and its significance, common infective agents and their diagnostic implications, as well as the formation of pigments and minerals found in normal tissues and clinical conditions. Refreshments and Lunch will be provided. This course is accredited by the Institute of Biomedical Science 7 P a g e

8 Electron Microscopy & Neuropathology Theory & Practice June 2016 book now This Introductory workshop is run as a half-day session in conjunction with The Principles of Muscle Biopsy at Buxton Labs based in The Walton Centre NHS Foundation Trust, Liverpool, the only specialist hospital trust in the UK dedicated to providing comprehensive neurology, neurosurgery, spinal and pain management services. The workshop provides a basic introduction to the theory and application of electron microscopy in diagnostic neuropathology On completion of the workshop students will have a basic knowledge of How an electron microscope works Sample preparation including fixation and ultra-microtomy Applications in Neuropathology (nerve biopsy, muscle biopsy and pituitary tumours) Principles of nerve biopsy Preparation and staining of Nerve Biopsy On completion of the workshop students will have experienced Preparation of glass knives for ultra-microtomy Preparation of resin blocks for semi thin sections Cut, mount & stain a semi thin section QC sections under a light microscope Is this workshop the right one for me? This workshop is suitable for anyone who has a basic science background and wishes to gain an understanding of theory and practical experience in current electron microscopy, techniques, and those students & laboratory personnel completing the IBMS Specialist Portfolio in Cellular Pathology. It will consist of a series of lectures, demonstrations and some limited practical experience. The session is taught over half a day, with the other half comprising the Principles of Muscle Biopsy. Refreshments and Lunch will be provided. This course is accredited by the Institute of Biomedical Science 8 P a g e

9 The Principles of Muscle Biopsy Theory & Practice June 2016 book now This Introductory workshop is run as a half-day session in conjunction with Electron Microscopy and Neuropathology at Buxton Labs based in The Walton Centre NHS Foundation Trust, Liverpool, the only specialist hospital trust in the UK dedicated to providing comprehensive neurology, neurosurgery, spinal and pain management services. The workshop provides a basic introduction to the structure of muscle and the theory and preparation of muscle biopsy. On completion of the workshop students will understand Types of muscle The structure of muscle Theory and application of Muscle Biopsy Orientation of muscle tissue Cutting and staining of muscle tissue On completion of the workshop students will have experienced Orientation of muscle tissue, (using a piece of steak) Visualising stained muscle sections using Light Microscopy Is this workshop the right one for me? This workshop is suitable for anyone who has a basic science background and wishes to gain an understanding of theory and practical experience in the principles of Muscle Biopsy and those students & laboratory personnel completing the IBMS Specialist Portfolio in Cellular Pathology. It will consist of a series of lectures and a lab based practical session and is taught over half a day, with the other half comprising Electron Microscopy and Neuropathology. Refreshments and Lunch will be provided. This course is accredited by the Institute of Biomedical Science 9 P a g e

10 Immunocytochemistry Beginners / Refresher Theory & Practice April & Sept 2016 book now This introductory workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which boasts a purpose built cellular pathology laboratory as part of their state of the art facilities for students and workshop delegates. The workshop will provide a basic introduction and training in immunocytochemistry theory, practice and techniques used in cellular pathology laboratories, through the use of presentations, discussion and laboratory based practical sessions. On completion of the workshop students will have a basic knowledge of History and background of ICC staining Immunofluorescence Development of ICC techniques (Direct, Indirect, PAP, APAAP, streptavidin/biotin, ABC) Extended polymer labelled detection methods Chromogens and substrates Use of positive and negative controls Use of blocking serums Background staining Antibody titre Antigen retrieval techniques EQA of ICC within the clinical environment 10 P a g e

11 On completion of the workshop students will be able to Understand the principles of immunocytochemistry Understand the rationale of immunocytochemical reactions Identify positive staining in tissue sections Identify good, poor and indifferent immunocytochemical staining Identify and understand the causes of background staining and how to eliminate it Explain the use of control sections and their importance Use antibodies and reagents following immunocytochemical staining protocols Understand the importance of correct antibody titre Understand the principles of antigen retrieval Is this workshop the right one for me? This workshop is suitable for anyone who has a basic science background and wishes to gain an understanding of theory and practice in immunocytochemistry techniques and those students & laboratory personnel completing the IBMS Specialist Portfolio in Cellular Pathology. The workshop will provide the knowledge for participants to recognise, assess and trouble-shoot both immunocytochemistry stained sections together with other techniques used in diagnostic cellular pathology. Refreshments and Lunch will be provided. This course is accredited by the Institute of Biomedical Science 11 P a g e

12 Immunocytochemistry Intermediate / Troubleshooting Theory & Practice April & September 2016 book now This specialist workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which boasts a purpose built cellular pathology laboratory as part of their state of the art facilities for students and workshop delegates. The workshop will provide an in depth focus on immunocytochemistry, its applications and troubleshooting within cellular pathology laboratories, through the use of presentations, discussion and practical based sessions. On completion of the workshop students will have a basic knowledge of Diagnostic antibody utility in conjunction with modern detection and visualisation technologies The development and role of automation within the clinical environment Factors effecting instrument selection Safeguards to ensure robust clinical services The rationale behind visualisation techniques The benefits of the use of different detection systems and visualisation techniques Pitfalls and common issues in immunocytochemistry Troubleshooting and eliminating or minimising errors Application of detection and visualisation methods as prognostic indicators Key clinical conditions where these demonstration methods may be of diagnostic value The value of panels in immunocytochemistry for the diagnosis of disease EQA of ICC within the clinical environment 12 P a g e

13 On completion of the workshop students will be able to Select the appropriate antibodies for the demonstration of components of the commonly stained tissues Identify positive staining Clearly distinguish between positive, negative and equivocal results Resolve problems associated with detection and visualisation methods Evaluate the staining of new antibodies and methodologies Assess the quality of stained slides Assess stained slides for artefacts Identify and understand the causes of artefacts and how to eliminate them Apply detection and visualisation methods as prognostic indicators Participate in the submission and evaluation of relevant EQA results Is this workshop the right one for me? This workshop is suitable for anyone who has a specialist science background and wishes to gain an in depth understanding of immunocytochemistry, its applications and troubleshooting within cellular pathology laboratories and those students & laboratory personnel completing the IBMS Specialist Portfolio in Cellular Pathology. The workshop will provide the specialist knowledge for participants to recognise, assess and trouble-shoot both immunocytochemistry stained sections for diagnostic cellular pathology. Refreshments and Lunch will be provided. Delegates are invited to bring their own slides for troubleshooting if desired. This course is accredited by the Institute of Biomedical Science 13 P a g e

14 Non Gynae Cytology Beginners / Refresher Theory & Practice May & August 2016 book now The introductory workshop is run as a full day at the Queen Elizabeth Hospital Gateshead. The workshop provides a basic introduction to non gynae cytology, principles of preparation and cell morphology through the use of presentations, discussion and practical microscopy sessions. On completion of the workshop students will have a basic knowledge of Types of non gynae sample and where they come from Principles of non gynae preparation Quality including NEQAS scheme and assessment Troubleshooting Expected cells in benign samples Frequently seen malignancies in non gynae samples On completion of the workshop students will have experienced through practical microscopy sessions Morphology of benign and malignant cells in non gynae samples Quality assessment and NEQAS criteria Troubleshooting specific preparation and staining problems Is this workshop the right one for me? This workshop will give a basic overview of non gynae cytology, and will look at sample types, preparation methods, cell morphology and quality issues. It is intended for those who are new to non gynae preparation and pre-screening. It may be particularly useful for those who prepare non gynae samples but do not get the opportunity to pre-screen or for those who have recently taken over non gynae cytology services as a result of laboratory reconfiguration. This course is accredited by the Institute of Biomedical Science 14 P a g e

15 Registration Form Cellular Pathology Education Workshop *******PLEASE COMPLETE AND RETURN ASAP TO SECURE YOUR PLACE ****** Submission of this completed Registration Form is essential to secure a place on the course. ALL SECTIONS MUST BE COMPLETED IN FULL All workshops are held at the University of Northumbria University, UK* *With the exception of Neuro / Muscle workshops which will be held at the Walton Centre, Liverpool, and the Non Gynae Cytology Beginners / Refresher workshop which will be held at QEH, Gateshead. Workshops available Please indicate below, in the appropriate box, which workshops you wish to register for. Please note if booking more than one place on the same workshop you will need to provide us with a separate Registration Form for each individual. The details of the individual attending the workshop must be completed in the Required Information for Booking Section, overleaf. Workshop Title Date Cost Special Staining Beginners / Refresher 22nd June VAT Attending (please tick as appropriate) Specialist Workshop A 23rd June VAT Specialist Workshop B Non Gynae Cytology Beginners / Refresher Non Gynae Cytology Beginners / Refresher Muscle / Neuropathology and Electron Microscopy Introductory Muscle / Neuropathology and Electron Microscopy Introductory ICC Beginners / Refresher ICC Intermediate ICC Beginners / Refresher ICC Intermediate 11th August VAT 5th May VAT 10th August VAT 15th June VAT 16th June VAT 6th April VAT 7 th April VAT 7th Sept VAT 8 th Sept VAT 15 P a g e

16 Registration Form Cellular Pathology Education Workshop *******PLEASE COMPLETE AND RETURN ASAP TO SECURE YOUR PLACE ****** Submission of this completed Registration Form is essential to secure a place on the course. ALL SECTIONS MUST BE COMPLETED IN FULL Accommodation Please note this is not a residential course, but accommodation may be provided at an additional supplement of 85 per night B&B. Please indicate below, in the appropriate box, if you do or do not require accommodation. If accommodation is required please indicate, in the appropriate box, if accommodation is required for the night before or after the workshop, or both. I do not require accommodation I will require accommodation Night prior to the workshop Night following the workshop Dietary requirements Price per person includes lunch and refreshments. If you have any special dietary requirements please indicate below, in the appropriate box, and we will do our best to accommodate them. YES ( please give details) No Required Information for booking Please complete all of the required details below. Your registration form will be returned if sections of this form are left blank and a place will not be reserved for you. PURCHASE ORDER (PO) NUMBER Please provide for invoicing for payment (please indicate in the box opposite) UK NEQAS for CPT Participation Number Please indicate your Participation Number if your centre is subscribed to the Scheme (you must complete this to enable us to register you on a workshop) Please note, whilst we do understand the delays which may be caused in waiting for your supplies department to issue a PO number, a PO number is essential for invoicing and unfortunately we can no longer reserve places on workshops without this information. 16 P a g e

17 Registration Form Cellular Pathology Education Workshop *******PLEASE COMPLETE AND RETURN ASAP TO SECURE YOUR PLACE ****** Submission of this completed Registration Form is essential to secure a place on the course. ALL SECTIONS MUST BE COMPLETED IN FULL Required Information for booking (cont.) If you are booking more than one place on the same workshop you will need to provide us with a separate Registration Form for each individual Individual attending the workshop Name: address: Department: Hospital/ Laboratory: Address: Details where invoice is to be sent (if different from those of the attending individual) Contact name: address: Department: Hospital/ Laboratory: Address: Postcode: Postcode: Tele No: Tele No: address of the individual booking the workshop if different from those of the attending individual: Please note: If a workshop booking is cancelled within one calendar month of the course date then an administration fee of 50 will be charged. After completing the form please return to cellpathtech@ukneqas.org.uk for confirmation of your place on the course. Title your CPT Workshop booking. For any additional queries please also contact as above. DO NOT SEND PAYMENT WITH THIS FORM, AN INVOICE WILL BE ISSUED FOLLOWING RECEIPT 17 P a g e

18 Contact Us UK NEQAS for CPT personnel are happy to help with any bookings or requests for further information For more information or to book a place please contact UK NEQAS for CPT: Susan Mulinda Laura Gamblin Tel: +44 (0) Tel: +44 (0) E: cellpathtech@ukneqas.org.uk E: cpt@ukneqas.org.uk Providing worldwide external quality assessment and proficiency testing for all aspects of tissue diagnostics UK NEQAS for Cellular Pathology Technique cpt@ukneqas.org.uk The Pathology Centre, Queen Elizabeth Hospital, Sheriff Hill, Gateshead, Tyne and Wear NE9 6SX Tel: +44 (0) P a g e