Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines, Middlesex, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines, Middlesex, TW18 3HR, UK Microbiology Department Fewston Wing Harrogate District Hospital Lancaster Park Road Harrogate North Yorkshire HG2 7SX Contact: Ms Anne Hancox Tel: +44 (0) Fax: +44 (0) Website: Testing performed at the above address only DETAIL OF ACCREDITATION TISSUES Swabs (nasal, wound, neonatal, throat, mouth, genital, eye, ear, superficial wounds & skin), corneal scrapings, sputum, pleural fluid, bronchoalveolar samples, urine, tissues and biopsies, CSF, synovial fluids, fluids from normally sterile sites, intravascular cannulae General isolation and characterisation of micro-organisms of clinical significance In house documented methods following related PHE Standards for Microbiology Investigations (SMIs) and incorporating manufacturers instructions where relevant Manual culture, Nasal swabs MB-LP-BSOP5 Wound swabs MB-LP-BSOP14 Neonatal screening swabs MB-LP-BSOP23 Throat swabs MB-LP-BSOP9 Mouth swabs MB-LP-BSOP4 Genital swabs MB-LP-BSOP28 Eye swabs & cannicular pus MB- MB-LP-BSOP2 Corneal scrapings MB-LP-BSOP52 Ear swabs MB-LP-BSOP1 Superficial wounds and skin MB-LP-BSOP11 Using manual culture, Investigation of sputum, pleural fluid, BAL & associated specimens MB-LP-BSOP57 Investigation of Urine MB-LP-BSOP41 Assessment Manager: LD1 Page 1 of 6
2 TISSUES Swabs (nasal, wound, neonatal, throat, mouth, genital, eye, ear, superficial wounds & skin), corneal scrapings, sputum, pleural fluid, bronchoalveolar samples, urine, tissues and biopsies, CSF, synovial fluids, fluids from normally sterile sites, intravascular cannulae Faeces Whole blood Bacterial culture isolates produced by the laboratory General isolation and characterisation of micro-organisms of clinical significance Isolation and characterisation bacterial pathogens: Salmonella sp. Shigella spp. Campylobacter spp. Escherichia coli O157 Vibrio spp. Yersinia spp. Detection of microbial growth and general isolation and characterisation of micro-organisms of clinical significance Antibiotic susceptibility testing Investigation of tissues and biopsies MB-LP-BSOP17 Investigation of CSF MB-LP-BSOP27 Investigation of synovial fluids MB-LP-BSOP102 Investigation of Fluids from Normally Sterile Sites MB-LP-BSOP 26 Investigation of Intravascular cannulae MB-LP-BSOP122 Manual culture Investigation of faeces for pathogens MB-LP-BSOP30 Liquid culture using Bactec 9240 MB-LP-BSOP37 BSAC (manual disc diffusion) MB-LP-BSOP45 Assessment Manager: LD1 Page 2 of 6
3 TISSUES Bacterial and fungal culture isolates produced by the laboratory Identification of micro-organisms of clinical significance API kits, MB-LP-BSOPTP62 Catalase test MB-LP-BSOPTP8 coagulase test MB-LP-BSOPTP10 DNase test MB-LP-BSOPTP12 oxidase tests MB-LP-BSOPTP26 chromogenic culture MB-LP-BSOP29, staining/microscopy MB-LP-BSOP109 Germ tube test MB-LP-BSOPTP51 Prolex Streptococcal Grouping kit MB-LP-BSOPTP52 Urease test MB-LP-BSOPTP61 Supporting bacterial identification SOPs: MB-LP-BSOPID 2, 3, 4, 5, 6A, 6B, 6C, 7, 8, 11, 12, 13, 14, 16, 17, 18, 20, 22, 24, 25, 26 & 27 Nose swabs Isolation of MRSA Manual chromogenic culture MRSA screening swabs MB-LP-BSOP29 Urine CSF Detection and quantification of red cells, white cells, epithelial cells, bacteria and yeasts Detection, identification and quantification (inc. differential) of: White blood cells Red blood cells Bacterial and fungal cells UF1000 by flow cytometry Investigation of urine MB-LP-BSOP41 MB-LP-BSOP145 Microscopy (stained and unstained, inc. Gram and differential) MB-LP-BSOP27 Synovial fluid Presence of crystals Microscopy MB-LP-BSOP102 Assessment Manager: LD1 Page 3 of 6
4 TISSUES Sputum, Pleural fluid, bronchoalveolar samples (urgent samples only) Urine Urine & CSF Respiratory samples Faeces Screening for acid-alcohol fast bacilli Detection of Legionella pneumophila serotype 1 antigen Detection of pneumococcal soluble Antigen Detection of RSV fusion protein antigen Detection of Clostridium difficile toxins A & B and GDH antigen Microscopy (urgent only) using auramine stains Investigation of Specimens for Mycobacterium sp MB-LP-BSOP40 Staining Procedures MB-LP-BSOP109 Manual immunochromatographic membrane assays: Binax Now kit by Alere MB-LP-VSOP 5 Binax Now kit by Alere MB-LP-VSOP 6 Binax Now kit by Alere MB-LP-VSOP 7 Techlab Quik Chek Complete kit by Alere MB-LP-BSOPTP66 Faeces Rota/Adenovirus antigen detection GastroVir strip by Coris MB-LI-BSOP30A Molecular diagnostic examination activities for the purpose of clinical diagnosis Urines and genital swabs Detection of Chlamydia trachomatis and Neisseria gonorrhoea rrna Hologic Panther Aptima Combo 2 assay Molecular: transcription mediated amplification of the RNA target detected by hybridisation and real time fluorescence MB-LP-MSOP3 Assessment Manager: LD1 Page 4 of 6
5 TISSUES Serum Serological examination activities Detection of HIV antigen/antibody, HBV surface antigen, HBV surface antibody, HCV antibody, Hepatitis A IgM antibody, Rubella IgG antibody, syphilis IgG antibody Siemens Centaur XPi analyser Immunoassays using chemiluminescence technology MB-LP-VSOP16 Serum Gentamicin concentration Siemens Centaur XPi analyser Immunoassays using chemiluminescence technology MB-LP-VSOP16 Serum Faeces Detection of measles IgG antibody, toxoplasma IgM/IgG antibody, Lyme disease IgM/IgG antibody to Borrelia burgdorferi, CMV IgM antibody, EBV IgM antibody, Hepatitis A total antibody Parasitology examination activities Isolation and identification of faecal ova, cysts and parasites. Mycology examination activities for the purpose of clinical diagnosis BioMerieux VIDAS analyser Immunoassays using enzyme linked fluorescent assay MB-LP-VSOP14 Concentration method and microscopy MB-LP-BSOP31 Hair, skin and nails Examination for superficial mycoses Manual culture MB-LP-BSOP39 Potassium hydroxide test & microscopy MB-LP-BSOPTP30 Assessment Manager: LD1 Page 5 of 6
6 HUMAN BODY FLUIDS Semen Andrology examination activities for the purpose of clinical diagnosis Infertility assessment: Semen volume, viscosity and appearance Sperm Concentration Sperm Motility Sperm Morphology All techniques carried out manually with cell counting chambers and microscopy according to WHO (2010) guidelines MB-LP-SSOP1 Post-vasectomy Semen samples Sperm presence/absence MB-LP-SSOP2 END Assessment Manager: LD1 Page 6 of 6
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