Medical Microbiology Microscopic slides and media

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Medical Microbiology Microscopic slides and media"

Transcription

1 Medical Microbiology Microscopic slides and media Head of Microbiology Department and Laboratory Medical Immunology : Janina Grzegorczyk MD, PhD, professor Implementators: Małgorzata Brauncajs MD Zbigniew Krzemiński MD, PhD, professor

2 Class 1. General microbiology Morphology of bacterial cell, staining procedures for bacteria, culture media for bacteria, cultivation and isolation techniques for bacteria, preparation of pure cultures. Loeffler medium (slant for the detection of corynebacteria) Enriched media Loewenstein-Jensen medium (slant for the detection of mycobacteria)

3 Class 1. General microbiology Morphology of bacterial cell, staining procedures for bacteria, culture media for bacteria, cultivation and isolation techniques for bacteria, preparation of pure cultures. The pigment does not diffuse into the culture medium (Micrococcus sp., Staphylococcus sp.) Chromogenous bacterial strains The pigment diffuses into the culture medium (Pseudomonas sp.) 3

4 Class 1. General microbiology Morphology of bacterial cell, staining procedures for bacteria, culture media for bacteria, cultivation and isolation techniques for bacteria, preparation of pure cultures. Negatively stained Bacillus sp. cylindrical shape Microscopic slides negative stain Negatively stained cocci spherical shape 4

5 Class 1. General microbiology Morphology of bacterial cell, staining procedures for bacteria, culture media for bacteria, cultivation and isolation techniques for bacteria, preparation of pure cultures. Microscopic slide capsule Positive-negative stain 5

6 Class 1. General microbiology Morphology of bacterial cell, staining procedures for bacteria, culture media for bacteria, cultivation and isolation techniques for bacteria, preparation of pure cultures. A stained preparation of Bacillus subtilis showing endospores as uncoloured and the vegetative cell as violet (Gram endospore stain) Microscopic slides endospores A stained preparation of Bacillus subtilis showing endospores as green and the vegetative cell as red (Schaeffer-Fulton endospore stain) 6

7 Class 1. General microbiology Morphology of bacterial cell, staining procedures for bacteria, culture media for bacteria, cultivation and isolation techniques for bacteria, preparation of pure cultures. Microscopic slides flagella 7

8 Class 3. General microbiology Identification and classification of bacteria according to their biochemical and antigenic properties, determination of bacterial resistance to the drugs. Identification of bacteria based on its biochemical properties API test 8

9 Class 3. General microbiology Identification and classification of bacteria according to their biochemical and antigenic properties, determination of bacterial resistance to the drugs. α-hemolysis (incomplete/green hue) Two types of hemolysis β-hemolysis (complete) 9

10 Class 3. General microbiology Identification and classification of bacteria according to their biochemical and antigenic properties, determination of bacterial resistance to the drugs. Disc diffusion test 10

11 Class 3. General microbiology Identification and classification of bacteria according to their biochemical and antigenic properties, determination of bacterial resistance to the drugs. Etest, Epsilometer test (MIC minimal inhibitory concentration); MIC = 0,125 11

12 Class 3. General microbiology Identification and classification of bacteria according to their biochemical and antigenic properties, determination of bacterial resistance to the drugs. MRSA Methicillin-resistant Staphylococcus aureus Determination of S. aureus sensitivity to methicillin with cefoxitin disc Determination of S. aureus sensitivity review method 12

13 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Identification of staphylococci in selective-differential medium mannitol salt agar (Chapman s medium). Mannitol-positive staphylococci (S.aureus) a change in the pigmentation of the medium from pink to yellow can be seen; mannitol-negative staphylococci (S.epidermidis) no change in the pigmentation 13

14 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Rapid distinction between micrococci and staphylococci with furazolidone disc: micrococci on the left side (resistant), staphylococci on the right side (susceptible) 14

15 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Staphylococcus aureus Gram stain (Gram-positive cocci forming structures similar to wine grapes) 15

16 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). The bacitracin (SP disc) test for identifying Streptococcus pyogenes 16

17 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). The optochin (OP disc) test for identifying Streptococcus pneumoniae 17

18 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Streptococcus pyogenes Gram stain (Gram-positive cocci forming long chains) 18

19 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Streptococcus pneumoniae in tissue Gram stain (Gram-positive cocci forming diplococci with capsule) 19

20 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Enterococcus faecalis Coccosel-agar medium. 20

21 Class 5. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Enterococcus faecalis Gram stain (Gram-positive cocci forming pairs or short chains) 21

22 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Neisseria gonorrhoeae Gram-stained urethral discharge 22

23 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Treponema pallidum Giemsa stain (spiral shape) 23

24 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). C.diphtheriae Neisser stain (violet storage cells) C.diphtheriae Gram stain (Gram-positive, highly pleomorphic organisms with no particular arrangement) 24

25 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). C.pseudodiphteriticum Neisser stain (without storage cells) C.pseudodiphteriticum Gram stain (Grampositive rods, frequently show club-shaped swellings and palisade arrangement) 25

26 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Actinomyces israelii Gram stain (Gram-positive bacteria that form long, branching filaments resembling the fungi) 26

27 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Growth of the species belonging to Mycobacterium in the Loewenstein-Jensen medium: (test tube nr 1 Nocardia spp. agar slant; test tubes nr 2 and 3 mycobacteria in the L-J medium) 27

28 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Bogen s test 28

29 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Biochemical tests for mycobacteria (test tube nr 1 niacin test, nr 2 Nocardia spp. slant, test tube nr 3 Bogen s test) 29

30 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Niacin test 30

31 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). Mycobacterium tuberculosis Ziehl-Neelsen stain: (acid-fast, red-stained mycobacteria) 31

32 Class 6. Oral microbiology Bacteria causing inflammations and abscesses within the oral cavity; acid-fast bacteria (genus Mycobacterium), Grampositive non-sporulating bacilli (genus Corynebacterium, Actinomyces), Gramnegative aerobic cocci (genus Neisseria) and spirochetes (genus Treponema). The antimicrobial susceptibility of Mycobacterium tuberculosis H37Rv 32

33 Class 8. Oral microbiology Bacteriology of dental caries and periodontal diseases. Bacteroides sp. (Gram-negative bacteria,short, pleomorphic rods) Anaerobic Gram-negative bacteria Gram stain. Fusobacterium sp. ( Gram-negative bacteria, thin, long, usually sharp-ended rods) 33

34 Class 8. Oral microbiology Bacteriology of dental caries and periodontal diseases. Anaerobic Gram-positive bacteria Gram stain Propionibacterium sp. (Gram-positive bacteria, cylindrical, pleomorphic cells) 34

Laboratory Exercise # 11: Differentiation of the Species Staphylococcus and Streptococcus

Laboratory Exercise # 11: Differentiation of the Species Staphylococcus and Streptococcus Laboratory Exercise # 11: Differentiation of the Species Staphylococcus and Streptococcus Purpose: The purpose of this laboratory exercise is to explore the differences between Staphylococcal species and

More information

Cocci Coccobacilli Bacilli Curved or curled Vibrios Spirilla Spirochete. Mycoplasma. Identification Colored stains

Cocci Coccobacilli Bacilli Curved or curled Vibrios Spirilla Spirochete. Mycoplasma. Identification Colored stains 1 Bacteria size and organization Smallest living single celled organisms 0.2-2.0 microns 0.000002-0.000002 meters Chlamydia and Rickettsia Prokaryotic organization Nucleoid chromosome with no membrane

More information

Bacterial Classification and Disease

Bacterial Classification and Disease Bacterial Classification and Disease Purpose To provide an overview of how physicians think when confronted with a bacterial infection To alert you to the importance of bacterial classification in treatment

More information

Adapted from Biology 15 Laboratory Supplemental Manual: Wrightsman, Ininns and Cannon- Moloznic.

Adapted from Biology 15 Laboratory Supplemental Manual: Wrightsman, Ininns and Cannon- Moloznic. Biology 3B Laboratory Cultural Characteristics of Bacteria Objectives: Describe bacterial structure: colony morphology, cell shape, growth patterns. To distinguish how various growth media will affect

More information

BIOL 3200-005 Unknown Lab Report. Unknown #545. Enterococcus faecalis

BIOL 3200-005 Unknown Lab Report. Unknown #545. Enterococcus faecalis BIOL 3200-005 Unknown Lab Report Unknown #545 Enterococcus faecalis Henry G. Norrell III (Manny) Lab: Tuesdays 3:00-4:50 pm 2 December 2014 INTRODUCTION: This experiment was performed to identify an unknown

More information

Medical microbiology is the study of interactions between humans and microorganisms such as bacteria, viruses, fungi and parasites.

Medical microbiology is the study of interactions between humans and microorganisms such as bacteria, viruses, fungi and parasites. Commensal and Pathogenic Microbial Flora in Humans Medical microbiology is the study of interactions between humans and microorganisms such as bacteria, viruses, fungi and parasites. Although the primary

More information

Normal flora, which make up about 90% of the cells of a human body, are microbes that

Normal flora, which make up about 90% of the cells of a human body, are microbes that Indigenous Unknowns Lab Report Introduction: Normal flora, which make up about 90% of the cells of a human body, are microbes that live and grow on the body without causing disease under normal conditions.

More information

SELECTIVE AND DIFFERENTIAL MEDIA

SELECTIVE AND DIFFERENTIAL MEDIA SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. Selective media allow certain types of organisms to grow, and inhibit the growth

More information

IDENTIFICATION OF OTHER UNKNOWN BACTERIAL SPECIES: OU

IDENTIFICATION OF OTHER UNKNOWN BACTERIAL SPECIES: OU IDENTIFICATION OF OTHER UNKNOWN BACTERIAL SPECIES: OU I. OBJECTIVES To demonstrate the capacity to utilize previous laboratory experiences to accurately interpret tests conducted to identify a certain

More information

14 The ability of the lenses to distinguish fine detail and structure is called a. Illumination b. Magnification c. Refractive index d.

14 The ability of the lenses to distinguish fine detail and structure is called a. Illumination b. Magnification c. Refractive index d. 1 2 Assume you stain Bacillus by applying malachite green with heat and then counterstain with safranin. Through the microscope, the green structures are a. cell walls. b. capsules. c. endospores. d. flagella.

More information

Laboratory Exercise #3. Isolation and identification of Unknown Bacteria. Procedure for Week 1 of Exercise 3. Day 1:

Laboratory Exercise #3. Isolation and identification of Unknown Bacteria. Procedure for Week 1 of Exercise 3. Day 1: Laboratory Exercise #3 Isolation and identification of Unknown Bacteria Procedure for Week 1 of Exercise 3 Day 1: 1. You will have 5 broth cultures at your workstation. Three of these tubes will contain

More information

STAINING AND BACTERIAL CELL MORPHOLOGY. To learn the techniques of Gram staining, nigrosin staining and KOH test.

STAINING AND BACTERIAL CELL MORPHOLOGY. To learn the techniques of Gram staining, nigrosin staining and KOH test. STAINING AND BACTERIAL CELL MORPHOLOGY I. OBJECTIVES To learn the technique of smear preparation. To learn the techniques of Gram staining, nigrosin staining and KOH test. To use and relate the Gram stain

More information

BLOOD & CATHETER TIP CULTURES GENERAL PROCEDURE

BLOOD & CATHETER TIP CULTURES GENERAL PROCEDURE University of Nebraska Medical Center Division of Laboratory Science Clinical Laboratory Science Program CLS 418/CLS 419 BLOOD & CATHETER TIP CULTURES GENERAL PROCEDURE I. Principle Microorganisms present

More information

BIO203 Laboratory Media and Biochemical Tests

BIO203 Laboratory Media and Biochemical Tests BIO203 Laboratory Media and Biochemical Tests Table of Contents I. Media 1 TSA Tryptic Soy Agar 1 Blood Agar 2 EMB Eosin Methylene Blue Agar 3 MSA Mannitol Salt Agar 4 MacConkey Agar 5 II. Colony Morphology

More information

Labquality External Quality Assessment Programmes General Bacteriology 1 4/2010

Labquality External Quality Assessment Programmes General Bacteriology 1 4/2010 Labquality External Quality Assessment Programmes General Bacteriology 1 4/2010 Photos and text: Markku Koskela, M.D., Ph.D. Clinical microbiology specialist Oulu, Finland Sample 13/2010 Cerebrospinal

More information

Escherichia coli (E. coli) primary stain

Escherichia coli (E. coli) primary stain Lab Exercise #3 - INSTRUCTIONS Identification of Unknown Bacteria Differential Staining and Specialized Bacterial Growth Media Lab Exercise #3 INSTRUCTIONS I. OBJECTIVES: Provide the student with an opportunity

More information

Sampling of the surface contamination using sterile cotton swabs from toys obtained from

Sampling of the surface contamination using sterile cotton swabs from toys obtained from RESULTS Sampling of the surface contamination using sterile cotton swabs from toys obtained from the Nursery at Queen Mary, University of London showed diverse microorganism growth. A variety of species

More information

Interpretation of Mastitis Milk Culture Results

Interpretation of Mastitis Milk Culture Results Interpretation of Mastitis Milk Culture Results Individual Cow Samples Contagious Pathogens-Infection with contagious pathogens occurs from the milk of other infected animals. These infections can be prevented

More information

ENTEROCOCCUS MODULE 17.1 INTRODUCTION OBJECTIVES 17.2 ENTEROCOCCI. Notes

ENTEROCOCCUS MODULE 17.1 INTRODUCTION OBJECTIVES 17.2 ENTEROCOCCI. Notes 17 ENTEROCOCCUS 17.1 INTRODUCTION is a genus of lactic acid bacteria. Enterococci are catalase negative Gram-positive cocci that often occur in pairs (diplococci) or short chains, and are difficult to

More information

Objectives. Basic Microbiology. The Basics. Identification. Jim Gauthier, Providence Care A Webber Training Teleclass

Objectives. Basic Microbiology. The Basics. Identification. Jim Gauthier, Providence Care A Webber Training Teleclass Objectives Basic Microbiology Jim Gauthier Welcome to the Bug Man s World! And yes, it s a Small World! Be at ease with the terminology Understand normal vs. abnormal flora Demystify all the Latin and

More information

Medical Microbiology Culture Media :

Medical Microbiology Culture Media : Lecture 3 Dr. Ismail I. Daood Medical Microbiology Culture Media : Culture media are used for recognition and identification (diagnosis) of microorganisms. The media are contained in plates (Petri dishes),

More information

Course Specification. University: Al-Azhar Faculty: Medicine Department: Medical Microbiology. 1- Data of the course: 2- Objectives o the course:

Course Specification. University: Al-Azhar Faculty: Medicine Department: Medical Microbiology. 1- Data of the course: 2- Objectives o the course: University: Al-Azhar Faculty: Medicine Department: Medical Microbiology 1- Data of the course: Course Specification Code of the course: 304-mic Specialty: Medical Microbiology Title of the course: medical

More information

URINE CULTURES GENERAL PROCEDURE

URINE CULTURES GENERAL PROCEDURE University of Nebraska Medical Center Division of Laboratory Science Clinical Laboratory Science Program CLS 418/CLS 419 URINE CULTURES GENERAL PROCEDURE I. Principle Urine cultures are performed to detect

More information

BACTERIAL IDENTIFICATION TESTS

BACTERIAL IDENTIFICATION TESTS 11 BACTERIAL IDENTIFICATION TESTS 11.1 INTRODUCTION In the previous chapter we have discussed various methods of isolation of bacteria. The bacteria thus isolated needs to be further identified to genus

More information

2014 Summer II Nursing Microbiology Laboratory Biology 2460

2014 Summer II Nursing Microbiology Laboratory Biology 2460 2014 Summer II Nursing Microbiology Laboratory Biology 2460 Graduate TA: Email: Office Hours/Location: Class Day, Time & Location: M & W 1:00 4:50 p.m. Class Location: LSB: 342 (Section 002) Lab Manual:

More information

Raw Milk Quality Tests Do They Predict Fluid Milk Shelf-life or Is it time for new tests?

Raw Milk Quality Tests Do They Predict Fluid Milk Shelf-life or Is it time for new tests? Raw Milk Quality Tests Do They Predict Fluid Milk Shelf-life or Is it time for new tests? Martin Wiedmann Milk Quality Improvement Program November 3, 2011 Fluid milk shelf life What defines shelf life

More information

Oxygen relation Definition Examples Picture Facultative Anaerobe

Oxygen relation Definition Examples Picture Facultative Anaerobe Oxygen relation Definition Examples Picture Facultative Anaerobe Does not require oxygen. Can grow with or without it. Able to detoxify toxic by products of oxygen. E. Coli Microaerophile Growth throughout

More information

USE OF THE GRAM STAIN FOR DIAGNOSIS OF INFECTIOUS DISEASE

USE OF THE GRAM STAIN FOR DIAGNOSIS OF INFECTIOUS DISEASE USE OF THE GRAM STAIN FOR DIAGNOSIS OF INFECTIOUS DISEASE Part One: Introduction and Cell Types Washington C. Winn, Jr., M.D. Clinical Microbiology Laboratory Department of Pathology University of Vermont

More information

The Oral Microflora. M.R.Milward School of Dentistry

The Oral Microflora. M.R.Milward School of Dentistry The Oral Microflora M.R.Milward School of Dentistry Contents 1. Definitions 2. Microbial habitats 3. Factors affecting growth 4. Identification 5. Important oral bacteria 6. Clinical examples 7. Flora

More information

CONSPECT MEDICAL MICROBIOLOGY 2013 / 2014

CONSPECT MEDICAL MICROBIOLOGY 2013 / 2014 CONSPECT MEDICAL MICROBIOLOGY 2013 / 2014 1. Objective and aims of microbiology. Development of microbiology. 2. Bacterial taxonomy. 3. Classification and nomenclature. 4. Bacterial morphology and cytoplasmic

More information

LAB 4. Cultivation of Bacteria INTRODUCTION

LAB 4. Cultivation of Bacteria INTRODUCTION LAB 4. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth

More information

Stimulating Participation and Learning in Microbiology: Presence and Identification of Bacteria from Student s Hands

Stimulating Participation and Learning in Microbiology: Presence and Identification of Bacteria from Student s Hands Teaching and Learning Tips Stimulating Participation and Learning in Microbiology: Presence and Identification of Bacteria from Student s Hands Patrícia Antunes Abstract: We proposed in the Basic Microbiology

More information

Lesson 10. At the Bacteriology Laboratory (II)

Lesson 10. At the Bacteriology Laboratory (II) Lesson 10 Mrs. Riemer : Good morning, Keiko. Keiko : Good morning. Mrs. Riemer : As you know, this is the student bacteriology lab. We're going to practice various bacteriological examination tests here.

More information

Funky Fomites and Aseptic Microbiology Techniques for Bacterial Isolation

Funky Fomites and Aseptic Microbiology Techniques for Bacterial Isolation Funky Fomites and Aseptic Microbiology Techniques for Bacterial Isolation Written by Jackie Hoffman Annotation: This laboratory exercise includes both a lecture on the purpose of practicing aseptic technique

More information

PACKAGE LEAFLET. CLINDAMYCIN capsules Clidamycin. One capsule of 75 mg contains 75 mg Clindamycin (as hydrochloride).

PACKAGE LEAFLET. CLINDAMYCIN capsules Clidamycin. One capsule of 75 mg contains 75 mg Clindamycin (as hydrochloride). PACKAGE LEAFLET CLINDAMYCIN capsules Clidamycin COMPOSITION One capsule of 75 mg contains 75 mg Clindamycin (as hydrochloride). One capsule of 150 mg contains 150 mg Clindamycin (as hydrochloride). PROPERTIES

More information

LAB 3 Bacterial Staining Techniques II I. Differential Stains: Gram Stain and Acid-fast Stain II. Morphological Unknown

LAB 3 Bacterial Staining Techniques II I. Differential Stains: Gram Stain and Acid-fast Stain II. Morphological Unknown LAB 3 Bacterial Staining Techniques II I. Differential Stains: Gram Stain and Acid-fast Stain II. Morphological Unknown I. DIFFERENTIAL STAINS A. Gram Stain B. Acid-fast Stain A. Gram Stain The previous

More information

CHAPTER 3 OBSERVING MICROORGANISMS THROUGH A MICROSCOPE. I. UNITS OF MEASUREMENT - See Table 3.1 in text. + Fig. 3.2

CHAPTER 3 OBSERVING MICROORGANISMS THROUGH A MICROSCOPE. I. UNITS OF MEASUREMENT - See Table 3.1 in text. + Fig. 3.2 CHAPTER 3 OBSERVING MICROORGANISMS THROUGH A MICROSCOPE I. UNITS OF MEASUREMENT - See Table 3.1 in text. + Fig. 3.2 II. MICROSCOPY: THE INSTRUMENTS A. COMPOUND LIGHT MICROSCOPY Figure 3.3 1. Have ocular

More information

The Microscopic Examination of Milk Characterization of Milk Bacteria and Cells

The Microscopic Examination of Milk Characterization of Milk Bacteria and Cells The Microscopic Examination of Milk Characterization of Milk Bacteria and Cells The microscope has been used to observe and count bacteria and somatic cells in raw milk since the early 1900 s. It has proven

More information

BD Modified CNA Agar BD Modified CNA Agar with Crystal Violet

BD Modified CNA Agar BD Modified CNA Agar with Crystal Violet INSTRUCTIONS FOR USE READY-TO-USE PLATED MEDIA PA-255082.02 Rev.: June 2003 BD Modified CNA Agar BD Modified CNA Agar with Crystal Violet INTENDED USE BD Modified CNA Agar is a selective medium for the

More information

Isolation and Identification of Bacteria Present in the Activated Sludge Unit, in the Treatment of Industrial Waste Water

Isolation and Identification of Bacteria Present in the Activated Sludge Unit, in the Treatment of Industrial Waste Water Iranian J. Publ. Health, Vol. 30, Nos. 3-4, PP. 91-94, 2001 Isolation and Identification of Bacteria Present in the Activated Sludge Unit, in the Treatment of Industrial Waste Water MK Sharifi-Yazdi 1,

More information

COLA Complexity How a laboratory isolates and identifies organisms will determine the complexity level of the laboratory.

COLA  Complexity How a laboratory isolates and identifies organisms will determine the complexity level of the laboratory. COLA FAST FACTS 29 1 of 3 GENERAL MICROBIOLOGY Requirements for COLA s Laboratory Accreditation and Community Hospital Laboratory Accreditation programs are underlined for your information. The reader

More information

LABORATORY EXERCISES. to accompany MICROBIOLOGY LABORATORY

LABORATORY EXERCISES. to accompany MICROBIOLOGY LABORATORY LABORATORY EXERCISES to accompany MICROBIOLOGY LABORATORY BIO 209 Professor Susan C. Kavanaugh Bluegrass Community & Technical College Spring 2010 INTRODUCTION TO CULTURING, MEDIA, AND ASEPTIC TECHNIQUES

More information

BY2012 Microbiology. Staphylococcus species

BY2012 Microbiology. Staphylococcus species BY2012 Microbiology Staphylococcus species Staphylococcus spp. Clusters of Gram-positive cocci Staphylococcus spp. Grape-like Clusters of Gram-positive cocci Blood Agar Plates Staphylococcus aureus Staphylococcus

More information

VPM 201: Veterinary Bacteriology and Mycology 17/10/2010. LABORATORY 9-10 : Bacillus, Clostridium and Mycoplasmas

VPM 201: Veterinary Bacteriology and Mycology 17/10/2010. LABORATORY 9-10 : Bacillus, Clostridium and Mycoplasmas VPM 201: Veterinary Bacteriology and Mycology 17/10/2010 LABORATORY 9-10 : Bacillus, Clostridium and Mycoplasmas A. BACILLUS AND CLOSTRIDIUM The genera Bacillus and Clostridium are gram-positive rods and

More information

FACULTY OF MEDICAL SCIENCE

FACULTY OF MEDICAL SCIENCE Doctor of Philosophy Program in Microbiology FACULTY OF MEDICAL SCIENCE Naresuan University 171 Doctor of Philosophy Program in Microbiology The time is critical now for graduate education and research

More information

Secondary bacterial infections complicating skin lesions

Secondary bacterial infections complicating skin lesions J. Med. Microbiol. Vol. 51 (2002), 808 812 # 2002 Society for General Microbiology ISSN 0022-2615 REVIEW ARTICLE Secondary bacterial infections complicating skin lesions ITZHAK BROOK Department of Pediatrics,

More information

NORMAL FLORA OF HUMAN BODY

NORMAL FLORA OF HUMAN BODY MODULE Normal Flora of Human Body 7 NORMAL FLORA OF HUMAN BODY 7.1 INTRODUCTION In a healthy human, the internal tissues, e.g. blood, brain, muscle, etc., are normally free of microorganisms. However,

More information

Respiratory Cultures. Section Four: CLS419 Clinical Microbiology II University of Nebraska Medical Center Clinical Laboratory Science Program

Respiratory Cultures. Section Four: CLS419 Clinical Microbiology II University of Nebraska Medical Center Clinical Laboratory Science Program CLS419 Clinical Microbiology II University of Nebraska Medical Center Clinical Laboratory Science Program Section Four: Respiratory Cultures CLS 419 Clinical Microbiology II Page 62 Respiratory Cultures

More information

Microscopy and Cellular Morphology

Microscopy and Cellular Morphology Microscopy and Cellular Morphology As we discussed in class, many organisms on the planet exist as single cells and are referred to as microorganisms bacteria, protozoans, among others. When a single microorganism

More information

EFFICACY OF AKACID AS DISINFECTANT

EFFICACY OF AKACID AS DISINFECTANT UNIVERSITÄTSKLINIK FÜR INNERE MEDIZIN I Abteilung für Infektionen und Chemotherapie Mikrobiologische Laboratorien 3P Univ. Prof. DDr. A. Georgopoulos Währinger Gürtel 18-20, A - 1090 Wien, Austria Tel.:

More information

Welcome to Implementing Inquirybased Microbial Project. Veronica Ardi, PhD

Welcome to Implementing Inquirybased Microbial Project. Veronica Ardi, PhD Welcome to Implementing Inquirybased Microbial Project Veronica Ardi, PhD Microbiology Laboratory Courses CourseSmart: ebook resources http://instructors.coursesmart.com/ Microbiology Laboratory Courses

More information

Chapter 3: Observing Microorganisms Through a Microscope

Chapter 3: Observing Microorganisms Through a Microscope Chapter 3: Observing Microorganisms Through a Microscope : The technology of making very small things visible to the naked eye. Units of Measurement: The metric system is used to measure microorganisms.

More information

BHARATI VIDYAPEETH DEEMED UNIVERSITY MEDICAL COLLEGE, PUNE. ( Department of Microbiology) PhD syllabus

BHARATI VIDYAPEETH DEEMED UNIVERSITY MEDICAL COLLEGE, PUNE. ( Department of Microbiology) PhD syllabus BHARATI VIDYAPEETH DEEMED UNIVERSITY MEDICAL COLLEGE, PUNE General Microbiology 1. Morphology of bacteria 2. Growth and nutrition of bacteria 3. Sterilization and disinfection 4. Culture media 5. Culture

More information

Diagnostic Medical Microbiology

Diagnostic Medical Microbiology Diagnostic Medical Microbiology Laboratory Manual Dr. Ph.D. Microbiology Medical Technology Department Islamic University-Gaza 2007 Table of Contents Introduction General View on the Parameters Used in

More information

Microbiology Overview

Microbiology Overview Microbiology Overview Interpretation of preliminary microbiology data Gram-positive cocci Aerobic In clusters Coagulase (+): Staphylococcus aureus Coagulase (-): Staphylococcus lugdunensis and other coagulasenegative

More information

The general structure of bacteria

The general structure of bacteria The general structure of bacteria The uni-cellular organisms Viruses Herpes virus, HIV, influenza virus The procaryotic organisms Escherichia, Salmonella, Pseudomonas Streptococcus, Staphylococcus, Neisseria

More information

Section 1: Clinical Microbiology Laboratory handbook Trauma and Orthopaedics

Section 1: Clinical Microbiology Laboratory handbook Trauma and Orthopaedics Section 1: Clinical Microbiology Laboratory handbook Trauma and Orthopaedics Guidance for use of the microbiology laboratory / specimen taking in patients with suspected infection related to T+O Approved

More information

IV ANTIBIOTICS FOR LYME DISEASE (Ceftriaxone, Cefotaxime sodium, Doxycycline, Penicillin G potassium)

IV ANTIBIOTICS FOR LYME DISEASE (Ceftriaxone, Cefotaxime sodium, Doxycycline, Penicillin G potassium) RATIONALE FOR INCLUSION IN PA PROGRAM Background Lyme disease is a tick-borne infectious disease caused by the Borellia species of spirochetes. Lyme disease is endemic to many regions throughout the United

More information

URINARY TRACT CULTURES

URINARY TRACT CULTURES URINARY TRACT CULTURES Assuming that there is no kidney infection (nephritis), urine comes out of the kidney sterile. The sterile urine moves down the ureters into the urinary bladder for storage. The

More information

IDENTIFICATION OF UNKNOWN BACTERIA

IDENTIFICATION OF UNKNOWN BACTERIA IDENTIFICATION OF UNKNOWN BACTERIA It is virtually impossible to identify bacteria based on physical characteristics alone. This is due to the fact that there are only a few basic shapes and physical features

More information

Biology 260 MICROBIOLOGY

Biology 260 MICROBIOLOGY p.1 Biology 260 MICROBIOLOGY Spring 2009 Hours lecture - MWF 10:00-10:50, SAM 203 lab - TTh 9:00-10:50, SAM 303 Instructor Lauren Yasuda, Ph.D. office: SAM 318 / phone: 587-6994 / e-mail: lyasud@sccd.ctc.edu

More information

RESISTANCE PATTERN OF URINARY TRACT INFECTION BACTERIAL ISOLATES TO SELECTED QUINOLONES

RESISTANCE PATTERN OF URINARY TRACT INFECTION BACTERIAL ISOLATES TO SELECTED QUINOLONES RESISTANCE PATTERN OF URINARY TRACT INFECTION BACTERIAL ISOLATES TO SELECTED QUINOLONES *A.R.M Momoh, *M.AC. Odike, * S. Olowo, **A.A. Momoh, **P.O. Okolo Department of *Pathological Sciences, College

More information

IDENTIFICATION OF UNKNOWN BACTERIA

IDENTIFICATION OF UNKNOWN BACTERIA IDENTIFICATION OF UNKNOWN BACTERIA It is virtually impossible to identify bacteria based on physical characteristics alone. This is due to the fact that there are only a few basic shapes and physical features

More information

Chapter 3. Methods of Culturing Microorganisms. Topics. Different types of media Different types of microscopy

Chapter 3. Methods of Culturing Microorganisms. Topics. Different types of media Different types of microscopy Topics Chapter 3 Methods of Culturing Microorganisms Microscope Methods of Culturing Microorganisms Different types of media Different types of microscopy A single visible colony represents a pure culture

More information

ATTEMPT OF MYCOPLASMA ISOLATION RATE DETERMINATION FROM BOVINE RESPIRATORY DISEASES IN EUROPE FROM 2002

ATTEMPT OF MYCOPLASMA ISOLATION RATE DETERMINATION FROM BOVINE RESPIRATORY DISEASES IN EUROPE FROM 2002 ATTEMPT OF MYCOPLASMA ISOLATION RATE DETERMINATION FROM BOVINE RESPIRATORY DISEASES IN EUROPE FROM 2002 Vallé M., Galland D., Broissier M., Woehrlé F., Boisramé B. Vetoquinol Research Centre Corresponding

More information

APPENDIX B: UWHC SURGICAL ANTIMICROBIAL PROPHYLAXIS GUIDELINES

APPENDIX B: UWHC SURGICAL ANTIMICROBIAL PROPHYLAXIS GUIDELINES APPENDIX B: UWHC SURGICAL ANTIMICROBIAL PROPHYLAXIS GUIDELINES Principles of prophylaxis 1) Use antimicrobials for surgical procedures where prophylactic antimicrobials have been found to be beneficial.

More information

Enteric Unknowns Miramar College Biology 205 Microbiology

Enteric Unknowns Miramar College Biology 205 Microbiology Enteric Unknowns Miramar College Biology 205 Microbiology Enteric (Greek enteron = intestine) bacteria are comprised of several different genera, but all reside in the digestive tract of mammals. Because

More information

NEW SELECTIVE AND DIFFERENTIAL MEDIUM FOR COAGULASE-POSITIVE STAPHYLOCOCCI ALLOWING RAPID GROWTH AND STRAIN DIFFERENTIATION'

NEW SELECTIVE AND DIFFERENTIAL MEDIUM FOR COAGULASE-POSITIVE STAPHYLOCOCCI ALLOWING RAPID GROWTH AND STRAIN DIFFERENTIATION' NEW SELECTIVE AND DIFFERENTIAL MEDIUM FOR COAGULASE-POSITIVE STAPHYLOCOCCI ALLOWING RAPID GROWTH AND STRAIN DIFFERENTIATION' SYDNEY M. FINEGOLD AND EDWARD E. SWEENEY Departments of Medicine, Veterans Administration

More information

A SIMPLE SCHEME FOR THE IDENTIFICATION OF MARINE HETEROTROPHIC BACTERIA

A SIMPLE SCHEME FOR THE IDENTIFICATION OF MARINE HETEROTROPHIC BACTERIA Thalassas, 2007, 23 (2): 17-21 An International Journal of Marine Sciences A SIMPLE SCHEME FOR THE IDENTIFICATION OF MARINE HETEROTROPHIC BACTERIA SURAJIT DAS*, P. S. LYLA & S. AJMAL KHAN Keywords: Marine

More information

Observing Microorganisms Through a Microscope

Observing Microorganisms Through a Microscope Chapter 3 Observing Microorganisms Through a Microscope Units of Measurement Microorganisms are measured by metric units unfamiliar to many of us. The micrometer ( m), formerly known as the micron, is

More information

Chapter 4 Functional Anatomy of Prokaryotic and Eukaryotic Cells

Chapter 4 Functional Anatomy of Prokaryotic and Eukaryotic Cells Chapter 4 Functional Anatomy of Prokaryotic and Eukaryotic Cells Comparing prokaryotic and eukaryotic cells: Overview Prokaryotic and Eukaryotic cells are chemically similar o Contain nucleic acids, proteins,

More information

Professional English for Clinical Microbiology Unit 2: Working with Bacteria

Professional English for Clinical Microbiology Unit 2: Working with Bacteria Introduction When we pick our culture from the incubator, we will have a grown culture. Growth appears as colonies on the plate surface. Once we have obtained any growth, work flow in the Microbiology

More information

Jari Hirvonen Hospital Microbiologist Vaasa Central Hospital Dep. of Microbiology. Italy, Padenghe sul Garda March 2013

Jari Hirvonen Hospital Microbiologist Vaasa Central Hospital Dep. of Microbiology. Italy, Padenghe sul Garda March 2013 Jari Hirvonen Hospital Microbiologist Vaasa Central Hospital Dep. of Microbiology Italy, Padenghe sul Garda March 2013 Vaasa Hospital District (VHD) Locates on the coast in western Finland Population ~170

More information

CHARACTERIZATION OF INDIGENOUS BACTERIA AND PATHOGENS

CHARACTERIZATION OF INDIGENOUS BACTERIA AND PATHOGENS Indigenous Bacteria and Pathogens 1 CHARACTERIZATION OF INDIGENOUS BACTERIA AND PATHOGENS All plants and animals are natural habitats for bacteria. The naturally occurring, and generally nonpathogenic

More information

HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY

HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results

More information

Antibiotics in sepsis

Antibiotics in sepsis Antibiotics in sepsis Jennifer Curello, PharmD, BCPS Antimicrobial Stewardship Program Ronald Reagan UCLA Medical Center September 13, 2013 Outline Sepsis definitions The sepsis bundle Timing of antimicrobials

More information

Biological Sciences Initiative

Biological Sciences Initiative Biological Sciences Initiative HHMI Student Activities Measuring Antibiotic Resistance Introduction: You might be aware that antibiotics were once thought of as a magic bullet; a nearly perfect drug for

More information

Antibiotic susceptibility

Antibiotic susceptibility Antibiotic susceptibility Antibiotic: natural chemicals produced by bacteria, fungi, actinomycetes, plants or animals, and either inhibits or kills other microbes and/or cells Chemotherapeutic agent: A

More information

Introduction. Introduction. Why do we need microbiological diagnostics of udder infections? Microbiological diagnostics How is it done?

Introduction. Introduction. Why do we need microbiological diagnostics of udder infections? Microbiological diagnostics How is it done? Introduction Microbiological diagnostics of udder infections Karin Persson Waller National Veterinary Institute (SVA) Swedish University of Agricultural Sciences Uppsala, Sweden Mastitis = in most cases

More information

HEAVY METAL TOLERANCE OF THREE DIFFERENT BACTERIA ISOLATED FROM INDUSTRIAL EFFLUENT

HEAVY METAL TOLERANCE OF THREE DIFFERENT BACTERIA ISOLATED FROM INDUSTRIAL EFFLUENT Ankita Shrivastava,, 2013; Volume 2(2): 137-147 HEAVY METAL TOLERANCE OF THREE DIFFERENT BACTERIA ISOLATED FROM INDUSTRIAL EFFLUENT ANKITA SHRIVASTAVA 1, VISHWAJEET SINGH 2, SADHANA JADON 2, SEEMA BHADAURIA

More information

Role of The Pharmaceutical Industry in Responsible Antibiotic Stewardship. Janet Hammond, MD, PhD Global Head Infectious Diseases, Roche pred

Role of The Pharmaceutical Industry in Responsible Antibiotic Stewardship. Janet Hammond, MD, PhD Global Head Infectious Diseases, Roche pred Role of The Pharmaceutical Industry in Responsible Antibiotic Stewardship Janet Hammond, MD, PhD Global Head Infectious Diseases, Roche pred The Problem Safe and effective antibiotics have saved billions

More information

Microorganisms encountered in routine pathology specimens

Microorganisms encountered in routine pathology specimens Technical Articles Role of Special Histochemical Stains in Staining Microorganisms Rashmil Saxena, BFA, HT(ASCP) CM Division of Transplantation Department of Surgery, Indiana University Indianapolis, IN,

More information

This laboratory introduces students to basic experiments and techniques used in the biochemistry

This laboratory introduces students to basic experiments and techniques used in the biochemistry Biochemistry Laboratory Introduction This laboratory introduces students to basic experiments and techniques used in the biochemistry laboratory. Initially, emphasis is placed on the buffer concept and

More information

Bacterial Classification, Structure and Function

Bacterial Classification, Structure and Function Frank Lowy Bacterial Classification, Structure and Function Introduction The purpose of this lecture is to introduce you to terminology used in microbiology. The lecture will: 1. cover different classification

More information

MEDICAL MICROBIOLOGY 603 OR POPULATION HEALTH SCIENCES 603 CLINICAL AND PUBLIC HEALTH MICROBIOLOGY January 18-May 6, 2011

MEDICAL MICROBIOLOGY 603 OR POPULATION HEALTH SCIENCES 603 CLINICAL AND PUBLIC HEALTH MICROBIOLOGY January 18-May 6, 2011 MEDICAL MICROBIOLOGY 603 OR POPULATION HEALTH SCIENCES 603 CLINICAL AND PUBLIC HEALTH MICROBIOLOGY January 18-May 6, 2011 Lectures: M, W, F 1:20-2:30 P.M. ( All lectures will be ~ 70 Minutes ) Office Hours

More information

R.KAVITHA, M.PHARM LECTURER DEPARTMENT OF PHARMACEUTIC SRM COLLEGE OF PHARMACY KATTANKULATUR

R.KAVITHA, M.PHARM LECTURER DEPARTMENT OF PHARMACEUTIC SRM COLLEGE OF PHARMACY KATTANKULATUR R.KAVITHA, M.PHARM LECTURER DEPARTMENT OF PHARMACEUTIC SRM COLLEGE OF PHARMACY KATTANKULATUR IDENTIFICATION METHOD The most important task of a bacteriology is to identify the pathogens from the clinical

More information

Dr. Khalil Yusef Rashid Abujheisha.

Dr. Khalil Yusef Rashid Abujheisha. Dr. Khalil Yusef Rashid Abujheisha. Place and date of birth: Hebron/Palestine, 08/08/1971. Nationality: Palestinian. Marital status: Married (5 kids). Telephone number: Saudia Arabia: 00966550903341 Email:

More information

Acute bacterial meningitis: Adults

Acute bacterial meningitis: Adults Acute bacterial meningitis: Adults Diagnosis CSF findings: o White blood cell count (neutrophil/lymphocyte predominance) and protein concentration are elevated o Opening pressure 200-500 mm h 2 o o Glucose

More information

BIOCHEMICAL TEST TO DISTINGUISH BETWEEN VARIOUS TYPES OF BACTERIA

BIOCHEMICAL TEST TO DISTINGUISH BETWEEN VARIOUS TYPES OF BACTERIA BIOCHEMICAL TEST TO DISTINGUISH BETWEEN VARIOUS TYPES OF BACTERIA 1. Catalase test : distinguish streptococci from staphylococci (if the catalase test is positive, it will be staph, but if it s negative

More information

A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci

A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci J. clin. Path. (1964), 17, 231 A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci E. J. L. LOWBURY, A. KIDSON, AND H. A. LILLY From the Medical Research Council

More information

Streptococcus pyogenes 1

Streptococcus pyogenes 1 40 2011 Streptococcus pyogenes 1 21 12 28 22 12 22 11 Streptococcus pyogenes Methicillin-resistant Staphylococcus aureus (MRSA) S. pyogenes b 19 S. pyogenes, MRSA S. pyogenes Enterococcus Streptococcus

More information

CEFA-DROPS AND CEFA-TABS

CEFA-DROPS AND CEFA-TABS Page 1 of 5 FORT DODGE ANIMAL HEALTH Division of Wyeth 800-5TH STREET N.W., P.O. BOX 518 FORT DODGE IA 50501 USA Telephone: 515-955-4600 Fax: 515-955-3730 Order Desk Telephone: 800-685-5656 Order Desk

More information

Biochemical identification of bacteria. Dr. Paul Ingram Infectious Diseases Physician (RPH) & Microbiologist (PathWest Laboratories)

Biochemical identification of bacteria. Dr. Paul Ingram Infectious Diseases Physician (RPH) & Microbiologist (PathWest Laboratories) Biochemical identification of bacteria Dr. Paul Ingram Infectious Diseases Physician (RPH) & Microbiologist (PathWest Laboratories) Outline Phenotypic vs genotypic tests Pros and cons of biochemical tests

More information

BD CLED Agar / MacConkey II Agar (Biplate)

BD CLED Agar / MacConkey II Agar (Biplate) PA-257562.01-1 - INSTRUCTIONS FOR USE READY-TO-USE PLATED MEDIA PA-257562.01 Rev.: Jan. 2016 BD CLED Agar / MacConkey II Agar (Biplate) INTENDED USE BD CLED Agar / MacConkey II Agar (Biplate) is used for

More information

Hot Topic: Anaerobic Culture 6/15/ Mayo Foundation for Medical Education and Research. All rights reserved.

Hot Topic: Anaerobic Culture 6/15/ Mayo Foundation for Medical Education and Research. All rights reserved. Welcome to Mayo Medical Laboratories Hot Topics. These presentations provide short discussion of current topics and may be helpful to you in your practice. Our speaker for this program is Dr. Jon Rosenblatt,

More information

GROUP 21 Description: Weakly Gram-Positive Nonsporlating Acid Fast Slender Rods Key differences are: acid fast, growth Examples: Mycobacterium

GROUP 21 Description: Weakly Gram-Positive Nonsporlating Acid Fast Slender Rods Key differences are: acid fast, growth Examples: Mycobacterium Bergey s Manual of Determinative Bacteriology All of the unknowns will fall into the following groups in Bergey's Manual of Determinative Bacteriology (The pink book on the shelf in the laboratory). GROUP

More information

QUAD PLATE USER S GUIDE

QUAD PLATE USER S GUIDE DQCI Services d b SSGN & SSGNC QUAD PLATE QUAD PLATE USER S GUIDE 5205 Quincy Street Mounds View, MN 55112 Phone: 763 785 0484 Fax: 763 785 0584 e mail: info@dqci.com Website: www.dqci.com INTRODUCTION

More information

Basic Microbiology. By the end of this chapter, you will be able to: Introduction. What are Micro-organisms? Chapter 3

Basic Microbiology. By the end of this chapter, you will be able to: Introduction. What are Micro-organisms? Chapter 3 Chapter 3 Basic Microbiology By the end of this chapter, you will be able to: l Name the types of micro-organisms that may be found in clean rooms l List the ways in which microbial contamination is carried

More information

INTRODUCTION TO BACTERIA

INTRODUCTION TO BACTERIA Morphology and Classification INTRODUCTION TO BACTERIA Most bacteria (singular, bacterium) are very small, on the order of a few micrometers µm (10-6 meters) in length. It would take about 1,000 bacteria,

More information

Metabolism Dr.kareema Amine Al-Khafaji Assistant professor in microbiology, and dermatologist Babylon University, College of Medicine, Department of

Metabolism Dr.kareema Amine Al-Khafaji Assistant professor in microbiology, and dermatologist Babylon University, College of Medicine, Department of Metabolism Dr.kareema Amine Al-Khafaji Assistant professor in microbiology, and dermatologist Babylon University, College of Medicine, Department of Microbiology. Metabolism sum of all chemical processes

More information