Journal of Food Science and Biotechnology. Extraction Technology of Intracellular Melanin and Its Biological Function from Plect a nia YM421

Transcription

1 Journal of Food Science and Biotechnology 28 No. 2 Vol. Mar : (2009) Plect ani a YM421,,, (, ) : YM421 Plect ani a YM421 A 3 B3 C2, 4 h,90, ;, 80 mg/ ml56 %, : ; ;; :Q : A Extraction Technology of Intracellular Melanin and Its Biological Function from Plect a nia YM421 YE Ming, ZHU Li, YAN G Liu, L I Shi2yan ( School of Biotechnology and Food Engineering, Hefei University of Technology, Anhui Hefei ,China) Abstract :In this manuscript, t he extraction process of melanin f rom Plectania YM421, and t he abilities of anti2ultraviolet ray irradiation, antioxidative on p hosp hatidylcholine and bean oil of t he melanin were st udied. The optimal p rocess parameter s for melanin ext ractio n f ro m Plectania YM421 was achieved : extraction times as 4 hours, extraction tempretrue as 90 and ratio of material to solution as The melanin exhibit s the strong ability of anti2ultraviolet ray irradiation, and the oxygen radical absorbance capacity of p hosp hatidylcholine was 56 % at 80mg/ ml of t he melanin. Furt hermore, t he melanin can highly inhibit oxidative activity of bean oil. Key words : Plectania, melanin, extraction, biological f unction,,, [1 ] [2-3 ] [ 4 ],,,, 10 %,,,,, : :( ) : (19592),,, com

2 Plect ani a s p YM421 ( ), 112 [5 ] : PDA g/ dl ; [6 ] : 215 g/ dl, K2 HPO g/ dl,mgso g/ dl,015 g/ dl, 014 g/ dl ; p H mm 30 ml 100 ml, r/ min 10 d,, 3, , 60 [7 ] 1) : (1) 1 200, p H, 90 3 h ; (2) 1 200, p H 3 h ; (3), p H 3 h ; (4), p H 015 h ;,5 000 r/ min 10 min,, nm 2),,3,L 9 (3 4 ) 114 E1 coil [ 8 ] 10 8 / ml E1 coil, 011 g/ L 10 4 / ml, 10 ml, 20 W 30 cm min, 011 ml,37 24 h, 115 [ 9 ] 5, 015 ml 10 %,1 ml, 115 ml 20 %, 115 ml 018 %( 111 % SDS) ;,95 60 min, 5 ml,, r/ min 10 min, 532 nm : A I = (1 - A T/ A C) 100 % A C,A T 116 [ 10 ] 011 g 50 ml, 215 ml 2 ( 2 3), 0125 ml, 015 min, 3 min,, 5 min, 580 nm p H 1,p H,, p H12,p H12, p H12 p H 1 ph Fig. 1 Effect of ph on melanin extraction , 60,, 90,90 2 Fig. 2 Effect of temperature on melanin extraction , 1 200, 1 200

3 2 : Plect ani a YM Fig. 3 Effect of ph on melanin extraction , 315 h, 315 h, B > C > A, A 3 B3 C2, A 3 B3 C2, A 3 B3 C2, 4 h,90, Tab. 2 Results of variance analysis F F Fig. 4 Effect of time on melanin extraction 21115,L 9 (3 4 ) 1 1 Tab. 1 Orthogonal design and results A B C D ,,,, 5, 01021, 4, 4 () g, = (/ ) 100 % = / = % 1 1 (3 h) 1 (70 ) 1 (1 150) (80 ) 2 (1 200) (90 ) 3 (1 250) (315 h) (4 h) k k k R R 2,B 5 Fig. 5 Effect of times on melanin extraction ,, E1 coil,1 min, ; 2 min, E1 coil;, 3 min, , 8 min, , E1 coil ,, 80 mg/ ml, 56 %

4 E1 coil Tab. 3 Protection of E1 coil against UV light by melanin / min ,,,12 h, E1 coil 3 min Tab. 4 Protection of E1 coil by different concentration mela2 nin after 3 min UV light irradiation / (g / L) Fig. 6 The antioxidative activities of melanin on phos2 phatidylcholine 7 Fig. 7 The antioxidative activities of melanin on bean oil 3 () ( p H), [11 ], Plect ani a YM421, Plect ani a YM421 E1 coil, Plect ani a YM421,,, ( References) : [ 1 ] Alois A B, Michael H W. Biosynthesis and functions of fungal melanins[j ]. Annual Review of Phytopathology, 1986, 24 : [ 2 ] Jacobson E S, Tinnell S B. Antioxidant function of fungal melanin[j ]. J Bacteriol, 1993, 175 : [ 3 ] Liu Y T, Sui M J, Ji D D, et al. Protection from UV irradiation by melanin of mosquitocidal activity of Bt var israeliensis [J ]. J Invertebr Pathol, 1993, 62 : [ 4 ] da Silva M B, Marques A F, Nosanchuk J D, et al. Melanin in the dimorphic fungal pathogen Paracoccidioides brasilien2 sis : effect s on phagocytosis, intracellular resistance and drug susceptibility[j ]. Microbes And Infection, 2006 (8) : [ 5 ],,,. [J ].,2007,28 (10) : YE Ming, TAN Wei, CH EN Hui, et al. Pilot study on culture conditions of lachnum and its producing pigment condi2 tions[j ]. Food Science, 2007, 28 (10) : (in Chinese) [ 6 ],,,. Cephalsos pori um s p. [J ].,2007,24 (2) : YAN G Duan2yun, L I Chen2yun, L IN Yong2cheng, et al. Metabolites of mangrove endophytic f ungus Ce p halos pori um s p.

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