EFFORT: Ecology from Farm to Fork Of microbial drug Resistance and Transmission. Sofia Duarte, on behalf of the EFFORT consortium

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1 EFFORT: Ecology from Farm to Fork Of microbial drug Resistance and Transmission Sofia Duarte, on behalf of the EFFORT consortium Nordic One Health Conference, Friibergh March 2014

2 EFFORT Facts Duration: 5 years (Dec Nov 2018) Funding programme: EU FP7/ Consortium: 10 countries, 20 institutions Project coordinator: Jaap Wagenaar - Utrecht University Faculty of Veterinary Medicine (UUVM) 2

3 Consortium 3

4 Objectives Understanding the epidemiology of AMR in the food chain Understanding the ecology of AMR in the microbial communities Understanding the relative contribution of the exposure routes of AMR from animals to humans Understanding the economic impact and animal welfare aspects of AMR in the food chain 4

5 Project plan 5

6 Overview WP1 phenotypic resistance antimicrobial consumption relevant meta-data 6

7 Overview WP1 In-depth study 2 countries Farm and slaughter plant Animals, workers, companion animals, environment Faecal, meat, water / air / soil /dust / surfaces Broilers Pigs Descriptive study 9 countries Farm Animals, environment Faecal, meat, dust, manure Chicken Pork Turkey Veal calves Fish Companion animals Wildlife 7

8 Overview WP1 WP2 Metagenomic analysis DNA purification Sequencing of AMR genes Identify AMR profiles Quantify AMR genes 8

9 WP1 Overview WP2 Metagenomic analysis WP3 Transfer of AMR genes Identify influential biophysical parameters Catalogue epidemic plasmids and their spread potential Identify genetic traits allowing adaptation and spread of plasmids Intervention strategies that influence horizontal gene transfer and AMR gene stability 9

10 WP1 Overview WP2 Metagenomic analysis WP4 Epidemiological studies Relationship between AMR occurrence and farm/retail/environment/animal characteristics across Europe Relationship between AMR patterns at farm/slaughter and determinants in two sentinel occupational populations WP3 Transfer of AMR genes 10

11 WP1 Overview WP2 Metagenomic analysis WP4 Epidemiological studies WP5 AM consumption Quantify AM consumption, biosecurity and welfare Identify management and biosecurity risk factors for AM consumption Identify AM consumption risk factors influencing AMR WP3 Transfer of AMR genes 11

12 WP1 Overview WP2 Metagenomic analysis WP4 Epidemiological studies WP5 AM consumption WP6 Intervention studies at poultry/pig farms Systematic review of interventions Reduce general AM use Stop use of critically important groups Describe the impact of interventions on: AM use ; AMR; animal health and welfare; farm management; economy WP3 Transfer of AMR genes 12

13 WP1 Overview WP2 Metagenomic analysis WP4 Epidemiological studies WP5 AM consumption WP6 Intervention studies at poultry/pig farms WP7 Macro-ecological model Quantify human exposure to AMR through food and animals in general population Attribute exposure of occupational groups to sources of AMR (farm and slaughterhouse) Integrate exposure assessment and source attribution models Integrate AMR gene transfer rates Assess impact of interventions WP3 Transfer of AMR genes 13

14 WP1 Overview WP2 Metagenomic analysis WP4 Epidemiological studies WP5 AM consumption WP6 Intervention studies at poultry/pig farms WP8 Economic impact Development of impact parameters and data collection Bio-economic models On-farm impact analysis including animal welfare and health Chain-model and report on analysis of strategies WP7 Macro-ecological model WP3 Transfer of AMR genes 14

15 WP1 Overview WP2 Metagenomic analysis WP4 Epidemiological studies WP5 AM consumption WP6 Intervention studies at poultry/pig farms WP8 Economic impact WP7 Macro-ecological model WP3 Transfer of AMR genes 15

16 WP 9: PROJECT DISSEMINATION AND TRAINING Tasks Website Dissemination plan + stakeholders mapping Inform relevant stakeholders Training Policy round tables Final EFFORT conference 16

17 Nordic One Health Conference, Friibergh March

18 The research leading to these results has received funding from the European Community's Seventh Framework Programme [FP7/ ] under grant agreement n Thank you for your attention! Any questions?