XL830 TEM Sample Preparation (Liftout) Guide. v.1013
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1 XL830 TEM Sample Preparation (Liftout) Guide v.1013
2 1. Deposit Pt Strap 2. Trench 1 st Side 3. Clean 1 st Side 4. Rotate 5. Trench & Clean 2 nd Side 6. Mill the Cleaver 7. Insert & Connect Probe Tip (2) 8. Transport Lamella to TEM Grid 9. Attach Lamella to Grid pt Disconnect the Probe Tip 11. Attach Lamella to Grid pt Thin the Lamella (2) Contents
3 Deposit Pt Strap 1. Viewing with the e-beam, bring sample to eucentric height. Align beams. 2. Tilt stage to 52 degrees. 3. Set ion current to 70 pa. 4. Insert Pt GIS via the Gas Injection menu (see right). 5. Unblank the ion beam. 6. Set magnification. 7. Focus image; take a single ion image ( 1I icon). 8. Using the milling icons, draw a solid box where you wish to deposit the platinum strap. Strap length should be between 5-20 um (slightly longer than intended final length of lamella). Strap width should be approx. 1 um. 9. Change material file to PT_LOW.mtr if it is not selected. 10. Adjust x,y,z dimensions to preference. 11. Take another single ion image; reposition Pt pattern if necessary. 12. Click Mill to deposit the platinum strap. 13. When completed, inspect with e-beam. 14. Retract the Pt GIS.
4 Trench: 1 st Side 1. Select ion beam current. See chart below. 2. Unblank i-beam; quickly focus over the milling site. 3. Capture a single ion image. 4. Draw the trench or steps pattern against the lower edge of the Pt strap. Overlap with the strap by ~100nm. In the Milling menu, set (x, y, z) and appropriate material file. 5. Click Mill icon when ready. 6. Check progress by periodically taking a single ion or single electron image. 7. When complete, press the Del key to delete the pattern. Before milling: pattern is positioned adjacent to Pt strap After milling: step trench
5 Clean: 1 st Side Now that the first face of the lamella is exposed, it is useful to clean the surface with a lower beam current. 1. Set ion beam current to approx % of that used for trenching. 2. Unblank the ion beam, focus. 3. Capture a single ion image. 4. Draw cleaning pattern against the exposed face of the lamella. See image. 5. In the Milling menu, correct (x, y, z) dimensions and material file if necessary. 6. Click the Mill icon to begin cleaning. 7. When finished, press Del key to delete the pattern. 8. Unblank the e-beam.
6 Rotate With the e-beam selected: 1. Set tilt to 0 degrees. 2. In the Stage menu, drop the stage to maximum clearance by selecting mm in the Z input box and clicking Go to. Click OK when presented with a dialog box. 3. In the Stage tab, confirm that Eucentric Rotation is enabled. 4. In the Stage menu, select Rel. In the Rotate input box. Select +180 degrees, and click Go to. 5. Bring the stage back to Eucentric Height; tilt to 52 degrees.
7 Trench & Clean: 2 nd Side 1. In the Beam Menu -> Ion Beam, select the current previously used for the first trench. 2. Unblank i-beam; quickly focus and stigmate if necessary. 3. Capture a single ion image. 4. Draw trench pattern; confirm (x, y, z) and material file as before (slide 4). 5. Click Mill Icon when ready, periodically observing progress with 1E or 1I scans. 6. Erase trench pattern when complete. 7. In the Beam menu, change the ion current to previous cleaning value. Focus, stigmate if necessary. 8. Capture a single ion image. 9. Draw cleaning pattern as before (slide 5). 10. Click Mill Icon when ready.
8 Mill the Cleaver 1. In the Stage Menu, set Tilt to 0 degrees. 2. In the Beam tab, set the ion beam current to 350 pa. A lower current is also fine, but will take longer to mill through the lamella. Ideal milling time for this step is between 1-5 minutes. 3. Unblank the ion beam and focus over the lamella. 4. Capture a 1I Single Ion Image. 5. Using the Milling Icons, draw three solid boxes as shown at right. 6. In the Milling menu, check the following: 1. The appropriate material file is selected (if a material file for your sample does not exist, simply choose Si.mtr). 2. The Parallel icon is illuminated. 3. Z (depth) is set to a reasonable depth: 1 um is appropriate for most lamellae. 7. Click Mill. 8. During milling, frequently capture 1E Single Electron Images. When you can plainly see that your pattern has milled through the lamella (see image), click the STOP icon. 9. Switch to e-beam view and tilt the stage back to 52 degrees.
9 Insert & Connect Probe Tip (1) 1. In the Stage Menu, select Rel. 2. In the Stage Menu Z input, type mm. The stage will drop. 3. In the Milling Menu, click Insert next to the option Pt GIS. The platinum gas insertion system will enter the chamber. 4. Click Insert next to Probe Tip. A dialog box will appear asking if you wish to insert multiple GIS systems. Click OK. The probe tip will appear. 5. In the Beam Menu, select an ion imaging current (1-70 pa). 6. With the i-beam, align the probe tip directly above the lamella. 7. The stage must now be incrementally raised to the probe tip. While raising the stage in steps, it is important to periodically return to the ion view and realign the probe tip with the lamella if necessary. 8. In the Stage Menu, enter Z = once, followed by the values listed below: Z: Z: Z: Z: Z: When the probe tip is both aligned vertically (ion view) and within 2-3 microns of the lamella, it is time to deposit platinum and connect the probe.
10 Insert & Connect Probe Tip (2) 1. In the Ion Beam view, select a current of 4 pa. Unblank the ion beam, focus, and capture a 1I single ion image. 2. As shown at right, draw a small box adjacent to the probe tip. Leave a small gap between the end of the probe tip and the pattern. Keep the pattern on top of the Pt strap; if the pattern overlaps onto the sides of the lamella, platinum will be deposited on the face of the cross section. Set the material file to PT_LOW.mtr. 3. Click Mill. 4. During milling, frequently capture 1E electron images, monitoring the growth of the deposited Pt. 5. When it is clear that the probe tip and platinum deposition have connected, click the STOP milling icon. 6. In the Beam Menu, select an ion beam current of 350 pa. Unblank, focus, capture a single ion image. 7. Draw a pattern to mill through the remaining handle of the cleaver we left in the last step (see right). This will free the lamella from the trench and allow us to transport it to the TEM grid. 8. Select the appropriate material file. Click Mill. 9. Monitor the milling with 1E single electron images. Stop the milling when it is clear that the handle has been milled through. Do not allow milling to go on longer than necessary. Too much redeposition can create difficulty in removing the lamella.
11 Transport Lamella to Grid 1. With the probe tip attached and the final cut made, it is time to lift the cross-section out of the trench. 2. In the Stage Menu, select Rel. 3. In the Z input box, type and click Go To. The stage will drop away from the lamella and probe tip. Do not attempt to move the stage in a smaller increment, it will very likely cause stage hysteresis and may destroy the lamella in the process! 4. Retract the probe tip with the lamella attached. 5. In the Stage Menu, 1. Return Tilt to 0 degrees. 2. Select Z: mm. 6. Drive the stage to the TEM grid holder. 7. Locate the TEM grid. In the Stage Tab, select Align X and draw a line along the length of the TEM grid. 8. Focusing on the tallest part of the grid, bring the stage to eucentric height. 9. With the stage tilted to 52 degrees, 1. In the Stage Menu, select Rel. followed by a Z value of mm. 2. In the Milling Menu, select Insert Pt GIS followed by Insert Probe Tip. 10. In the Beam Menu, select an imaging ion current (1-70 pa). 11. Using the ion beam view, align the probe tip and lamella with the edge of the grid (see images). 12. In the same way the stage was raised to connect the probe tip to the lamella, the stage must now be incrementally raised to place the lamella on the grid. As before, do this using a series of smaller z-steps until the lamella is within 2-3 microns of the placement site.
12 Attach Lamella to Grid pt With the lamella in place, return to ion beam view. 2. Select a current of 4 pa. Refocus and capture a 1I single ion image. 3. Draw a pattern (shown at right) adjacent to the right side of the lamella. Make sure the pattern is close to, but not touching or overlapping with, the lamella. 4. Select PT_LOW.mtr as the material file. 5. Select an appropriate Pt deposition height (depth). (You will be monitoring the Pt growth, so an inaccurate value will not cause issues. 3-5 microns is a generally acceptable range.) 6. Click Mill and periodically capture 1E single electron images to monitor the Pt growth. When the platinum has connected with the lamella (see images), you may stop the milling.
13 Disconnect the Probe Tip 1. In the Beam Menu, select an ion current of pa. Unblank the ion beam, focus on the probe-tip-lamella connection, and capture a single ion image. 2. Using the Line Tool in the Milling Icons, draw a line along the probe tip / Pt deposition connection (see image). 3. In the Milling Menu, select none as the material file, and set the milling time for seconds. 4. Click Mill. 5. When finished, unblank the e-beam to view. 6. If the connection has not been fully milled through, repeat the previous steps. 7. When the connection has clearly been cut (examine with both beams to be sure), Retract the probe tip and proceed to the next slide.
14 Attach Lamella to Grid pt Once the probe tip has been retracted, it is possible to deposit a second platinum leg on the left side of the lamella. 2. In the Beam Menu, select an ion beam current of 4 pa. 3. Unblank the ion beam, focus and capture a 1I single ion image. 4. Draw a solid box pattern on the left side of the lamella, taking care to place it adjacent to, but not in contact with, the side of the lamella. 5. Select PT_LOW.mtr as the material file, and select an appropriate deposition height (Z). 6. Click Mill. 7. Monitor the Pt growth by periodically capturing a 1E single electron image. 8. When the platinum has adequately connected to the lamella, stop the milling. 9. Retract the Pt GIS.
15 Thin the Lamella (1) 1. Once connected to the grid, it is important to further thin the cross-section until it is electron-transparent and can be used in for transmission electron microscopy. 2. In the Beam Menu, select an ion current of 11 pa. Unblank the ion beam, focus and capture a 1I single ion image. 3. Using the Cleaning pattern tool, draw a cleaning pattern along the face of the lamella (as you did during the earlier cleaning procedures). 4. In the Milling Menu, select the appropriate material file and Z (depth) value. 5. Mill. 6. After milling, unblank the e-beam to view the lamella. 7. In the Beam Menu, select 4 pa and repeat the previous steps. 8. If desired, you may further clean the cross section by repeating the steps a third time with a current of 1 pa.
16 Thin the Lamella (2) 1. Return stage tilt to 0 degrees. 2. In the Stage Menu, select Abs mode, and type Z = 10.0 mm. Select Go to. 3. In the Stage Menu, switch to Rel. mode and change Rotation (R) to +180 degrees. Select Go to. 4. The stage will rotate and return to the TEM grid location. 5. Bring the stage back to eucentric height. 6. With the stage tilted to 52 degrees, repeat the cleaning steps on the previous slide. 7. When the sample begins to take on a much brighter appearance with the e-beam, it is approaching electrontransparent thickness. The grid and lamella may now be transferred to the TEM.
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