Isabelle Viana PhD in Biotechnology and Biosciences. MSc in Genetics and Molecular Biology

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1 curriculum vitae Isabelle Viana PhD in Biotechnology and Biosciences MSc in Genetics and Molecular Biology Center for Vaccine Research University of Pittsburgh Biomedical Science Tower 3, Room 9052 Pittsburgh, PA 15261, USA T viana@pitt.edu RESEARCH PROFILE My work portfolio lies on the design and characterization of proteins, through the use of computational chemistry, structural bioinformatics, molecular biology and biophysical tools. Current interests address biomedical applications, more specifically rational design of conformation-based epitopes, epitope presentation and antigen-antibody interaction issues. EDUCATION 2014 to current Postdoctoral Researcher, Center for Vaccine Research, University of Pittsburgh, USA to 2014 Visiting Researcher, Center for Vaccine Research, University of Pittsburgh, USA. Projects: Biophysical characterization of epitope-scaffold proteins by means of Circular Dichroism, Surface Plasmon resonance and Nuclear Magnetic Resonance. Measurements of binding affinity constants of anti-dengue human polyclonal serum to viral dengue particles using Surface Plasmon Resonance. Advisors: Dr. Ernesto Marques University of Pittsburgh Dr. Jodi Craigo University of Pittsburgh 2011 to 2014 Ph.D. Biotechnology and Biosciences, Carlos Chagas Institute, Oswaldo Cruz Foundation, Brazil. Thesis: In silico Design of Conformational HIV-1 Epitopes based Top7- Scaffolds Advisors: Dr. Marco Aurélio Krieger Carlos Chagas Institute Dr. Ernesto Marques University of Pittsburgh Dr. Rafael Dhalia Aggeu Magalhães Institute 2009 to 2011 Master in Genetics and Molecular Biology, Federal University of Pernambuco, Brazil. Dissertation: Production and validation of recombinant antigens from HIV-1/2 and HTLV-1/2 viruses for cytometric bead array diagnostic test development. Advisor: Dr. Rafael Dhalia Aggeu Magalhães Institute 2005 to 2009 B.Sc. Biomedicine, Federal University of Pernambuco, Brazil. Monography: Polyclonal antibody production aiming the evaluation of DNA-based vaccine expression against the Yellow Fever virus. Advisor: Dr. Rafael Dhalia Aggeu Magalhães Institute

2 COMPLEMENTARY EDUCATION Biacore Basics. GE-Healthcare, Piscataway, New Jersey, XSEDE New User Training. Extreme Science and Engineering Discovery Environment, XSEDE, Pittsburgh, Writing a Successful XSEDE Allocation Proposal. Extreme Science and Engineering Discovery Environment, XSEDE, Pittsburgh, Protein Structure. University of São Paulo, USP, São Paulo, Brazil, Environmental Molecular Science. Ohio State University, OSU, Columbus, United States, April to July, Course of English. Federal University of Pernambuco, UFPE, Recife, Brazil, 2005 to Immune response and phylogeny of HIV-1. Brazilian Society of Immunology, São Paulo, Brazil, October, I Winter Course of Immunology. University of São Paulo, USP, São Paulo, Brazil, July, IV Course of Molecular Biology. Hematology and Hemotherapy Foundation of Pernambuco, HEMOPE, Recife, Brazil, April, II Course of Basic Genetics. University of Pernambuco, UPE, Recife, Brazil, February, French Course. Federal University of Pernambuco, UFPE, Recife, Brazil, February to December, Advanced Technologies of Molecular Virology and Immunology. Aggeu Magalhães Research Center, Recife, Brazil, March to July, German Course. Federal University of Pernambuco, UFPE, Recife, Brazil, February to June, Course of Medical and Molecular Genetics. Keizo Asami Immunopathology Laboratory, LIKA, Brazil, January, PROFESSIONAL EXPERIENCE 2014 current Post Doc at the Center for Vaccine Research, University of Pittsburgh, USA Trainee under supervision of the Dr. Ernesto Marques, Associate Professor of Infectious Diseases and Microbiology Department and Researcher of Center of Vaccine Research; and Dr. Jodi Craigo, Research Associate Professor of Microbiology and Molecular Genetics Department and Research Manager of Center of Vaccine Research Ph.D. Student, under supervision of Dr. Marco Krieger, Professor of Cellular and Molecular Biology, Carlos Chagas Institute and director of the Molecular Biology Institute of Paraná; Dr. Rafael Dhalia, Technologist Researcher, Aggeu Magalhães Research Center; and Dr. Ernesto Marques, Associate Professor of Infectious Diseases and Microbiology Department and Researcher of Center of Vaccine Research. Page 2

3 Master Degree Student, under supervision of Dr. Rafael Dhalia, Technologist Researcher, Aggeu Magalhães Research Center, and professor of Molecular Biology, Federal University of Pernambuco Instructor of the subject Human Genetics, Department of Genetics, Federal University of Pernambuco Trainee, at the clinical analysis laboratory, University Hospital, Federal University of Pernambuco Trainee, under supervision of the Professor Dr. Arquimedes Melo, Professor of Toxicology, Federal University of Pernambuco. MEETING ORGANIZATION Oswaldo Cruz Foundation, Co-organizer and instructor, Synthetic Biology Application, Rio de Janeiro, October 17-21, 2011 (Brazil). PUBLICATIONS 1. Viana, Isabelle FT; Dhalia, Rafael; Krieger, Marco; Marques JR., Ernesto TA; Lins, Roberto. Influence of Scaffold Stability and Electrostatics on Top7-Based Engineered Helical HIV-1 Epitopes. Lecture Notes in Computer Science, 8213, p , Viana, Isabelle FT ; Soares, Thereza A. ; Lima, Luciana FO ; Marques JR., Ernesto TA ; Krieger, Marco ; Dhalia, Rafael ; Lins, Roberto. De Novo Design of Immunoreactive Conformation-specific HIV-1 Epitopes based on Top7 Scaffold. RSC Advances: an international journal to further the chemical sciences, v.3, p , (DOI: /C3RA41562G) 3. G. Bezerra-Jr., A. Barison, V. S. Oliveira, L. Foti, M. A. Krieger, R. Dhalia, I. F. T. Viana and W. H. Schreiner. The mechanism of cysteine detection in biological media by means of vanadium oxide nanoparticles. Journal of Nanoparticle Research, v.14, p , Dhalia, R.; Maciel Jr., M.; Cruz, F.S.P.; VIANA, I.F.T.; Palma, M.L.; August, T.; Marques Jr., E.T.A. Membrane and envelope virus proteins co-expressed as lysosome associated membrane protein (LAMP) fused antigens: a potential tool to develop DNA vaccines against flaviviruses. Anais da Academia Brasileira de Ciências (Impresso)., v.81, p , VIANA, I. F. T. Jararaca (Bothrops) In: Envenamento: Uma Abordagem Clínica.1 ed.olinda : Livro Rápido - Elógica, 2008, v.1, p SELECTED ABSTRACTS 1. VIANA, I.F.T.; CARVALHO, R.V. and LINS, R.D. Predictive Biomolecular Modeling Applied to Protein Engineering and Proteomics. In: XLII Reunion de la Sociedade Argentina de Biofisica, December, 2013, Carlos Paz, Cordoba, Argentina. 2. BEZERRA JR, A. G., FOTI, L., KRIEGER, M. A., DHALIA, R., VIANA, I. F. T., BARISSON, A., OLIVEIRA, V. S., SCHREINER, W. The mechanism of cysteine detection in biological media by means of vanadium oxide nanoparticles In: XXXV National Meeting of Physics and Condensed Matter, 2012, Águas de Lindóia. Page 3

4 3. VIANA, I. F. T., SOARES, T., KRIEGER, M. A., MARQUES JR, E. T. A., DHALIA, R., LINS NETO, R. D. Rational design of chimerical antigens based on Top7 scaffold for HIV differential diagnostic purpose In: XVI Brazilian Simposium of Theoretical Chemistry, 2011, Ouro Preto MG. 4. VIANA, I. F. T., MELO, K. M. S., CRUZ, F. S. P., QUEIROZ, G. L., FOTI, L., KRIEGER, M. A., GOLDENBERG, S., MARQUES JR, E. T. A., Dhalia, Rafael. A HIGHLY SENSITIVE AND DYNAMIC MICROSPHERE-BASED IMMUNOFLUORESCENT ASSAY BASED ON P24 FROM HIV 1 AND 2 EXPRESSED IN ESCHERICHIA COLI In: XXXV Congress of the Brazilian Society for Immunology / III ESCI Extra Section in Clinical Immunology / Effector Immune Response and Viral Immunity, 2010, Porto Alegre - RS. 5. MELO, K. M. S., VIANA, I. F. T., MARQUES JR, E. T. A., DHALIA, R. CONSTRUCTION OF TETRAVALENT DENGUE ANTIGENS AND POLYCLONAL ANTIBODIES: DEVELOPMENT OF A NS1-BASED DIAGNOSTIC KIT CAPABLE OF DETECTING THE FOUR DENGUE VIRUS SEROTYPES In: XXXV Congress of the Brazilian Society for Immunology / III ESCI Extra Section in Clinical Immunology / Effector Immune Response and Viral Immunity, 2010, Porto Alegre - RS. 6. SOUZA, R. C. V., VIANA, I. F. T., CRUZ, F. S. P., MELO, A. B., MARQUES JR, E. T. A., DHALIA, R. HEPATITIS A OPTIMIZED DNA-based vaccines EVALUATION: antigen expression, processing and secretion analysis. XXI National Meeting of Virology, 2010, Gramado - RS. 7. PALMA, M. L., VIANA, I. F. T., CRUZ, F. S. P., DHALIA, R., MARQUES JR, E. T. A. Expression analysis of DNA constructs coding for NS1 and NS3 Yellow Fever proteins: cellular traffic analyses of wild type and lysossome associated membrane protein (LAMP) fused proteins. XXXIV Congress of the Brazilian Society of Immunology, 2009, Salvador-BA. 8. CRUZ, F. S. P., VIANA, I. F. T., MARQUES JR, E. T. A., DHALIA, R. Optimized DNAbased vaccine against Yellow Fever virus: development and antigen expression evaluation. XX National Meeting of Virology, Brasília-DF, VIANA, I. F. T., PALMA, M. L., CRUZ, F. S. P., CALZAVARA-SILVA, C.E., CORDEIRO, M.T., DHALIA, R., MARQUES JR, E. T. A. Production of polyclonal antibodies against Yellow Fever virus proteins: an important tool to evaluate DNA vaccines expression and to develop diagnostic kits. XXXIV Congress of the Brazilian Society of Immunology, 2009, Salvador-BA. 10. VIANA, I. F. T., PALMA, M. L., CRUZ, F. S. P., CALZAVARA-SILVA, C.E., DHALIA, R., MARQUES JR, E. T. A. Optimization of Yellow Fever Virus proteins for bacterial expression and polyclonal antibodies production: a powerful tool to evaluate viral DNAbased vaccine expression. XIX National Congress of Virology, 2008, Caxambú - Minas Gerais. 11. VIANA, I. F. T., CALZAVARA-SILVA, C.E., CRUZ, F. S. P., PALMA, M. L., MAIA, R. C. C., Bressan, R. F. C., AUGUST, T., DHALIA, R., MARQUES JR, E. T. A. Production of Policlonal Antibodies Against Yellow Fever Proteins: DNA Vaccine Expression Evaluation. First Pan-American Dengue Research Network Meeting, 2008, Recife. 12. PALMA, M. L., VIANA, I. F. T., CRUZ, F. S. P., DHALIA, R., MARQUES JR, E. T. A. Yellow Fever non-structural proteins expression and cellular traffic analysis: utilization of the lysosome-associated membrane protein (LAMP) signal to target antigens to the major histocompatibility class II compartment. XIX National Congress of Virology, 2008, Caxambú - Minas Gerais. Page 4

5 REFEREE ASSISTANCE FOR SCIENTIFIC JOURNALS Analyst - Royal Society of Chemistry. Advances - Royal Society of Chemistry. BioMed Central. Journal of Nanoparticle Research. ACADEMIC ADVISORIES Jéssica Gouveia Lira. Production and validation of recombinant antigens from HIV-1 virus aiming diagnostic purposes. Scientific Training Program Aggeu Magalhães Research Center. TECHNICAL SKILLS Knowledge of experimental methods in molecular biology, such as cloning, protein expression and purification, PCR, Western blot, ELISA and Cytometric Bead Array. Knowledge of experimental methods in physics, such as NMR, Circular Dichroism and Surface Plasmon Resonance, applied to characterization of protein structure and protein-protein interactions. Knowledge of DNA sequence design and optimization, computational modeling of biological systems and molecular dynamics simulation. I am also familiar with computer platforms (Unix, OS X), classical computational chemistry softwares and biomolecular databases. LANGUAGES English Working Knowledge (Advanced Level) Portuguese Native OTHER INTERESTS Music Classic piano and guitar. Page 5