Biology 3308: Microbiology Spring 2007 Lecture Syllabus

Transcription

1 Biology 3308: Microbiology Spring 2007 Lecture Syllabus Lecture Instructor: Dr. Melany Fisk Office: Rankin North (Annex) 321 Phone: Course web address: Office hours: MWF 9:00 11:00 or by appointment This course is an introduction to the biology of microorganisms, including phylogeny and diversity, growth, metabolism, and genetics. In the lecture component of the course, students will learn about microbial: metabolic diversity responsiveness to the environment influence on the environment interactions with humans There will be 3 exams plus a comprehensive final exam for the lecture component. There will also be discussion questions in lectures and short written assignments. Textbook Brock Biology of Microorganisms by Madigan, tinko, and Parker is the rental textbook for lecture component of the course. Microbial Life by Perry, Staley, and Lory is on reserve at Belk Library. Readings in the textbooks are supplemental to lecture materials. Exams will cover only material presented in lectures; the textbooks therefore are intended for reference, clarification, and for review. Web Course information, lecture outlines, review material, and lab handouts will be posted on the following webpage: Grading Lecture Exams: (100 points each *3) 300 Final Exam 100 Written Assignments (15 points each * 6) 90 Lecture total 490 points Total points in lecture will be added to total points in lab (280) for a course total of 770. Final grades will be calculated as a percentage of 770, as follows: A+ 100% A 92-99% A % B % B 82-87% B % C % C 72-77% C % D % D 62-67% D % F < 60%

2 Lecture Schedule (subject to change) Bio 3308, Spring 2007 Date Subject Reading (chapter.section) Brock 9 Introduction to the microbial world ;4.4 1 Microbial Life 11 Historical perspectives and current trends ; ; 11.8; Cell structure and function , 5 p Cell structure and function , 5 p Biosynthesis and cell assembly ,11 25 Population growth Exam 1 1 Energetics, metabolic diversity, and the chemistry of our environment 2.4; ; 5 pp Respiration 5.11, 5.12; Fermentation 5.10; Lithotrophy Phototrophy Carbon cycling and some practical applications ; pp ; Nitrogen fixation pp ; Exam 2 1 DNA, RNA, protein synthesis ; Gene expression: regulation and responsiveness to the environment Viruses: structure, function, and prokaryote interactions ; ,15 Spring Break 20 Genetic exchange and recombination Human-viral interactions ; Human-prokaryote interactions: our flora Native flora and non-specific defenses Pathogenic bacteria, infection process Exam Easter Holiday Protozoan and fungal pathogens 17 Epidemiology Emergence of disease Disease and environmental change 22 30

3 Biology 3308 Microbiology Spring 2007 Laboratory Syllabus Laboratory Instructors: Dr. Ece Karatan, laboratory coordinator and instructor Office: Rankin Science North 319 Lab: Rankin Science North 303 Phone: Course website: Office hours: to be announced Mr. cus McGinnis Office: Rankin Science North Office hours: to be announced Mr. Josiah Zayner Office: Rankin Science North Office hours: to be announced The laboratory in this course will be an introduction to pure culture work and quantification of bacterial activity and abundance. Students will learn: sterile technique enrichment and isolation metabolic assays enumeration and quantification In addition to learning basic techniques of microbiology, students will isolate and identify unknown organisms, experiment with bacterial growth and activity, and carry out their own independent testing questions of their own interests. Three formal lab reports, one short informal report, and one power point presentation will be required. Textbook Microbiological Applications: Laboratory Manual in General Microbiology, short version. Edition 9/e. H.J. Benson. In addition, you will be provided with several lab handouts which are either supplementary to the book or contain experiments not found in the book. Attendance Policy: Attendance to labs is mandatory. Failure to attend a lab will result in a 20 point deduction from the final grade. If you have to miss a lab you will have to notify the instructor prior to the lab and present documentation for the reason for your absence. You will also need to arrange with him/her for a time to make up the lab; however, in some cases this may not be possible.

4 Laboratory Format: Many of the laboratory exercises will not take up all of the allocated time, however most will require you to come back during other times to check your experiments. The laboratory door can be opened with a code (to be supplied on the first day the lab meets) punched on the keypad. Laboratory reports: All lab reports must be typed, use correct grammar and spelling. More information about how to write to reports and what they should include will be presented during the lab and in the lab handouts. Laboratory Notebook: Your laboratory notebook should contain details of all the experiments you do during each lab as well as results, conclusions, and answers to the questions in the lab handouts. You will need your lab book to write your lab reports therefore you should carefully record the necessary information. If the experiments are performed exactly as described by a handout or the lab book you can reference these and not put down details of the procedures. We will use a binder with loose pages for your lab notebook. Because we will be doing multiple experiments during each lab having a binder will allow you to keep track of all the experiments more easily. Furthermore, you can put in all the handouts in the binder. Every experiment should begin on a new page and all the information from one experiment should be on consecutive pages. Each experiment should have a title, a purpose, methods and results. Your lab notebook will be collected twice during the semester and graded according to whether the notebook is well organized and complete. Laboratory Safety: Absolutely no food or drink allowed in the laboratory. Long hair must be tied back. We will be using bunsen burners all the time. A lab coat (or some sort of outer clothing to protect your clothes) is recommended. No contaminated instruments (loops, needles, pipettes etc) can be laid on the lab bench. Broken glassware is put in the provided container. Solid waste (used plates, pipette tips etc.) must be put in the autoclave bags. Liquid cultures have to be put in the area in the back of the lab once you are done with them, so we can collect them and autoclave them later. DO NOT put anything with liquid in it in the autoclave bags. Grading Informal: Lab notebooks 1 st collection 20 2 nd collection 50 Unknown identification 30 Formal: Lab reports Enrichment and Isolation 30 Growth in Culture 40 Activity and Abundance 50 Power point presentation Independent Project 60 Laboratory total 280 points

5 Laboratory Schedule (subject to change) Bio 3308, Spring 2007 Date Subject Assignments Due Exercise # (Benson) 9/10 Lab 1. Basic techniques: Sterile technique, inoculation and transfers, isolation, preparation of media Lab 1, cont. Microscopes and Staining 8, 9, 18, 20 1, 11, 14, 15 24/25 30/31 Lab 2. Enrichment and Isolation Lab 3. Isolation of unknown organism Lab 2, cont. Enrichment and Isolation Lab 3, cont. Isolation of unknown Lab notebooks due 5/6 Lab 3, cont. Staining and morphology of unknowns Lab 2, cont. Verification of Enrichments Lab 4. Bacterial growth in batch culture Lab 3, cont. Biochemical tests of unknowns: Biooxidations Lab 3, cont. Biochemical tests of unknowns: Hydrolysis Lab 4. Data analysis: bacterial growth Lab 2 report due 37, 38 39, 40 Lab 5. Microbial quantification: Respiration 6/7 Lab 5, cont. Microbial quantification: Plate counts Lab 4 report due Spring Break Lab 5, cont. Data analysis Lab 6. Start independent Lab 3 informal report due Lab 6. Independent Projects-open lab Lab 5 report due. 3/4 Lab 6. Independent Projects-open lab 10/11 Easter Power point presentations on independent Lab 6 presentation Lab notebooks due

6 Date Lab 1: Techniques 9/10 Sterile technique, inoculation & transfers, isolation, media prep Lab 2: Enrichment and Isolation Lab 3: Unknown ID Lab 4: Growth in Culture Lab 5: Quantification Lab 6: Independent Projects Microscopes, staining 24/25 Lab notebooks due Enrichments for Bacillus and Staphylococcus Begin isolation of unknown organism 30/31 Isolation of Bacillus and Staphylococcus Continue isolation 5/6 Verify isolation of Bacillus and Staphylococcus Staining and morphology of unknowns Lab 2 report due Biochemical tests: biooxidations Begin growth curves Biochemical tests: hydrolysis, miscellaneous Data analysis, discussion Microbial quantification: respiration 6/7 Lab 4 report due Plate counts for bacterial enumeration Meet with instructor to discuss topics Begin Spring Break No lab Lab 3 informal report due Data analysis, discussion Open lab for Lab 5 report due Open lab for. 3/4 Open lab for 10/11 Easter-no lab Power point presentation On independent Lab notebook due