BY2012 Microbiology Bacterial Fimbriae & Adherence
|
|
- Katrina Cross
- 8 years ago
- Views:
Transcription
1 BY2012 Microbiology Bacterial Fimbriae & Adherence
2 Bacterial Fimbriae Fimbriae Flagella Colourised electron micrograph of an Escherichia coli cell bearing type 1 fimbriae
3 Escherichia coli with Type 1 Fimbriae Fimbriae Transmission electron micrograph of E. coli cell bearing type 1 fimbriae negative staining
4 Red Blood Cells (Erythrocytes) - Model System to Demonstrate Fimbrial Adhesion Colourised electron micrograph of red blood cells
5 Fimbrial Adherence to Red Blood Cells Adhesion Schematic of a bacterium with cell-surface fimbriae or adhesins (represented by the black tips) binding to host cell receptors (sugars of glycoproteins or glycolipids) (represented by the open circles) on the host cell surface
6 Fimbrial Adherence to Red Blood Cells Mannose RBC Adhesion Blocking Schematic of a bacterium with cell-surface fimbriae or adhesins (represented by the black tips) binding to host cell receptors (sugars of glycoproteins or glycolipids) (represented by the open circles) on the host cell surface. Addition of mannose in the case of type 1 fimbriae blocks adherence.
7 Fimbrial Adherence - Haemagglutination Assay Fimbriate bacteria Fimbriate bacteria causing cross-linking of red blood cells
8 Escherichia coli Type 1 Fimbriae Cross-Linking Red Blood Cells 0.1 μm RBC RBC NCTC 8623 Type 1 Fimbriae RBC Transmission electron micrograph of red blood cells agglutinated by E. coli NCTC Type 1 fimbriae are attached (arrows) to the surfaces of three RBCs, cross-linking these to give rise to haemagglutination.
9 Escherichia coli NCTC 8623 Mannose-Sensitive Haemagglutination by Type 1 Fimbriae No Mannose Plus Mannose Inhibition of fimbrial haemagglutination
10 Escherichia coli NCTC 8623 Not Expressing Type 1 Fimbriae No Mannose Plus Mannose Bacteria not expressing Type 1 fimbriae do not cause haemagglutination
11 Escherichia coli with K88 Fimbriae K88 fimbriae differ from Type 1 fimbriae. They are very fine, flexible fibrillar structures rather than rod-like structures like type 1 fimbriae. Shadowing technique Transmission electron micrograph
12 Escherichia coli K88 Fimbriae K88 fimbriae are very fine fibrillar structures rather than rod-like structures. Transmission electron micrograph negative staining
13 Escherichia coli K88 Fimbriae Mannose-Resistant Haemagglutination No Mannose Plus Mannose No inhibition of fimbrial haemagglutination The receptor for K88 fimbriae is not mannose but β-linked N-acetylhexosamine (HexNAc), either GlcNAc or GalNAc
14 Escherichia coli K88 Fimbriae Mannose-Resistant Haemagglutination With continued incubation increased clumping of RBCs No Mannose Plus Mannose No inhibition of fimbrial haemagglutination The receptor for K88 fimbriae is not mannose but β-linked N-acetylhexosamine (HexNAc), either GlcNAc or GalNAc
15 Saccharomyces cerevisiae - Model System to Demonstrate Type 1 Fimbrial Adhesion Yeast cell suspension viewed by phase contrast microscopy
16 A Schematic of the Structure of the Yeast Cell Wall S S Mannoprotein 40% GPI Anchor β1-3 glucan 50% Transmembrane Protein β1-6 glucan 8% Chitin 2%
17 Saccharomyces Cell Surface Features Protease treated 100 nm The outer cell wall layer is composed mainly of mannoproteins (dense zone arrowed), covalently bound to ß-1,6-glucan (light zone), can be removed by protease treatment (right hand panel)
18 Saccharomyces Mannoproteins [α1 6-Mannose] Module Wall Lattice GPI = Glycosyl phosphatidyl inositol
19 Agglutination of Yeast Cells by E. coli Bearing Type 1 Fimbriae Agglutination Broth-grown cells expressing type 1 fimbriae No Agglutination Agar-grown cells not expressing type 1 fimbriae
20 Agglutination of Yeast Cells by E. coli Bearing Type 1 Fimbriae Agglutination Broth-grown cells No Mannose No Agglutination Broth-grown cells with Mannose
Chapter 6: Antigen-Antibody Interactions
Chapter 6: Antigen-Antibody Interactions I. Strength of Ag-Ab interactions A. Antibody Affinity - strength of total noncovalent interactions between single Ag-binding site on an Ab and a single epitope
More informationThe Lipid Bilayer Is a Two-Dimensional Fluid
The Lipid Bilayer Is a Two-Dimensional Fluid The aqueous environment inside and outside a cell prevents membrane lipids from escaping from bilayer, but nothing stops these molecules from moving about and
More informationBacterial (Prokaryotic) Cell. Common features of all cells. Tour of the Cell. Eukaryotic Cell. Plasma Membrane defines inside from outside
www.denniskunkel.com Tour of the Cell www.denniskunkel.com Today s Topics Properties of all cells Prokaryotes and Eukaryotes Functions of Major Cellular Organelles Information, Synthesis&Transport,, Vesicles
More informationAn Overview of Cells and Cell Research
An Overview of Cells and Cell Research 1 An Overview of Cells and Cell Research Chapter Outline Model Species and Cell types Cell components Tools of Cell Biology Model Species E. Coli: simplest organism
More informationLecture 4 Cell Membranes & Organelles
Lecture 4 Cell Membranes & Organelles Structure of Animal Cells The Phospholipid Structure Phospholipid structure Encases all living cells Its basic structure is represented by the fluidmosaic model Phospholipid
More informationSome Immunological Test. Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras
Some Immunological Test Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras Alaa Faeiz Antigen -Antibody Reactions. Antigen antibody reactions are performed to determine the presence
More informationMicroscopy. MICROSCOPY Light Electron Tunnelling Atomic Force RESOLVE: => INCREASE CONTRAST BIODIVERSITY I BIOL1051 MAJOR FUNCTIONS OF MICROSCOPES
BIODIVERSITY I BIOL1051 Microscopy Professor Marc C. Lavoie marc.lavoie@cavehill.uwi.edu MAJOR FUNCTIONS OF MICROSCOPES MAGNIFY RESOLVE: => INCREASE CONTRAST Microscopy 1. Eyepieces 2. Diopter adjustment
More informationHiPer Blood Grouping Teaching Kit
HiPer Blood Grouping Teaching Kit Product Code: HTI008 Number of experiments that can be performed: 100 Duration of Experiment: Protocol: 15 minutes Storage Instructions: The kit is stable for 6 months
More information1. BLOOD GROUP SYSTEMS. Page 1. Haematology LECTURE 10. BLOOD GROUPS AND TRANSFUSIONS OVERVIEW. 1. Blood Group Systems
Undergraduate Course in Veterinary Clinical PathologySocrates Programme Haematology LECTURE 10. BLOOD GROUPS AND TRANSFUSIONS 10-1 OVERVIEW 1. Blood Group Systems 2. Blood group testing and cross-matching
More informationChapter 6. Antigen-Antibody Properties 10/3/2012. Antigen-Antibody Interactions: Principles and Applications. Precipitin reactions
Chapter 6 Antigen-Antibody Interactions: Principles and Applications Antigen-Antibody Properties You must remember antibody affinity (single) VS avidity (multiple) High affinity: bound tightly and longer!
More informationThe immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small
The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small molecules that can elicit an immune response when linked
More informationBasic Immunologic Procedures. Complex Serological Tests
Basic Immunologic Procedures Complex Serological Tests Amal Alghamdi 2014-2015 1 Classification of antigen-antibody interactions: 1. Primary serological tests: (Marker techniques) e.g. Enzyme linked immuonosorben
More informationUnexplained agglutination of stored red blood cells in Alsever s solution caused by. The gram-negative bacterium Serratia liquefaciens
O RIGINAL R EPORT Unexplained agglutination of stored red blood cells in Alsever s solution caused by the gram-negative bacterium Serratia liquefaciens I. MARTINCIC,C.MASTRONARDI,A.CHUNG, AND S. RAMIREZ-ARCOS
More informationHapten - a small molecule that is antigenic but not (by itself) immunogenic.
Chapter 3. Antigens Terminology: Antigen: Substances that can be recognized by the surface antibody (B cells) or by the TCR (T cells) when associated with MHC molecules Immunogenicity VS Antigenicity:
More informationUnderstanding DARZALEX Interference with Blood Compatibility Testing
Understanding DARZALEX Interference with Blood Compatibility Testing DARZALEX (Daratumumab) Results in a False Positive Indirect Coombs Test DARZALEX is a human monoclonal antibody for the treatment of
More informationCHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS
CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS See APPENDIX (1) THE PRECIPITIN CURVE; (2) LABELING OF ANTIBODIES The defining characteristic of HUMORAL immune responses (which distinguishes them from CELL-MEDIATED
More informationCh. 8 - The Cell Membrane
Ch. 8 - The Cell Membrane 2007-2008 Phospholipids Phosphate head hydrophilic Fatty acid tails hydrophobic Arranged as a bilayer Phosphate attracted to water Fatty acid repelled by water Aaaah, one of those
More informationChapter 18: Applications of Immunology
Chapter 18: Applications of Immunology 1. Vaccinations 2. Monoclonal vs Polyclonal Ab 3. Diagnostic Immunology 1. Vaccinations What is Vaccination? A method of inducing artificial immunity by exposing
More informationExercise 2. The Compound Light Microscope
6 Exercise 2 The Compound Light Microscope INTRODUCTION: Student Learning Objectives: After completing this exercise students will: a. Demonstrate proficient use of the microscope using low, high dry,
More informationBiological Sciences Initiative
Biological Sciences Initiative HHMI Student Activities Measuring Antibiotic Resistance Introduction: You might be aware that antibiotics were once thought of as a magic bullet; a nearly perfect drug for
More informationMicroscopes and the Metric System
Microscopes and the Metric System BIO162 Fall 2007 Sizes of Microorganisms: -Viruses: 0.01 0.3 um -Bacteria: 1 3 um -Fungi: 3 30 um -Protozoa: 5 1000 um 1 Measuring Microorganisms Ocular Micrometer The
More informationUses of 2D gel electrophoresis in recombinant protein production. Kendrick Laboratories, Inc www.kendricklabs.com
Uses of 2D gel electrophoresis in recombinant protein production Kendrick Laboratories, Inc www.kendricklabs.com Recombinant proteins are biologics What is a Biologic? Biological products are those derived
More informationEffects of Antibiotics on Bacterial Growth and Protein Synthesis: Student Laboratory Manual
Effects of Antibiotics on Bacterial Growth and Protein Synthesis: Student Laboratory Manual I. Purpose...1 II. Introduction...1 III. Inhibition of Bacterial Growth Protocol...2 IV. Inhibition of in vitro
More informationMake your own bacteria!
Make your own bacteria! Bacteria: a single-celled microorganism with no membrane-bound nucleus. Bacteria are found everywhere from soil to acidic hot springs. You can make your own bacteria to take home
More informationStudent name ID # 2. (4 pts) What is the terminal electron acceptor in respiration? In photosynthesis? O2, NADP+
1. Membrane transport. A. (4 pts) What ion couples primary and secondary active transport in animal cells? What ion serves the same function in plant cells? Na+, H+ 2. (4 pts) What is the terminal electron
More informationAntigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies
A Brief Review of Antibody Structure A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known
More informationBlood-Based Cancer Diagnostics
The Biotechnology Education Company Blood-Based Cancer Diagnostics EDVO-Kit 141 Store entire experiment at room temperature. EXPERIMENT OBJECTIVE: The objective of this experiment is to learn and understand
More informationAnnexin V-FITC Apoptosis Detection Kit
ab14085 Annexin V-FITC Apoptosis Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Apoptosis in living cells (adherent and suspension). This product is for research
More informationCounting and imaging bacteria using fluorescent microscopy & Electron Microscopy and Atomic Force Microscopy (AFM)
Counting and imaging bacteria using fluorescent microscopy & Electron Microscopy and Atomic Force Microscopy (AFM) Bruce E. Logan Kappe Professor of Environmental Engineering Department of Civil and Environmental
More informationChapter 16: Innate Immunity
Chapter 16: Innate Immunity 1. Overview of Innate Immunity 2. Inflammation & Phagocytosis 3. Antimicrobial Substances 1. Overview of Innate Immunity The Body s Defenses The body has 2 types of defense
More informationCell Structure and Function. Eukaryotic Cell: Neuron
Cell Structure and Function Eukaryotic Cell: Neuron Cell Structure and Function Eukaryotic Cells: Blood Cells Cell Structure and Function Prokaryotic Cells: Bacteria Cell Structure and Function All living
More informationAnnexin V-EGFP Apoptosis Detection Kit
ab14153 Annexin V-EGFP Apoptosis Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples This product is for research use only and is not intended
More informationPURIFICATION AND CHARACTERIZATION OF CELL WALL MANNOPROTEINS OF CANDIDA ALBICANS USING INTACT CELL METHOD
Medical Journal of the Islamic Republic of Iran Z. Farahnejad, et al. Volume 18 Number 2 Summer 1383 August 2004 PURIFICATION AND CHARACTERIZATION OF CELL WALL MANNOPROTEINS OF CANDIDA ALBICANS USING INTACT
More informationFeed Forward Loops in Biological Systems
Feed Forward Loops in Biological Systems Dr. M. Vijayalakshmi School of Chemical and Biotechnology SASTRA University Joint Initiative of IITs and IISc Funded by MHRD Page 1 of 7 Table of Contents 1 INTRODUCTION...
More informationAnnexin V-FITC Apoptosis Detection Kit
ab14085 Annexin V-FITC Apoptosis Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Apoptosis in living cells (adherent and suspension). This product is for research
More informationHow To Understand How Gene Expression Is Regulated
What makes cells different from each other? How do cells respond to information from environment? Regulation of: - Transcription - prokaryotes - eukaryotes - mrna splicing - mrna localisation and translation
More informationBlood Type Testing Lab Report Section 1101 Nattanit Trakullapphan (Nam) Chawalnrath Wongdeshanan (Kat)
Blood Type Testing Lab Report Section 1101 Nattanit Trakullapphan (Nam) Chawalnrath Wongdeshanan (Kat) Introduction: figure 1.1 (Blood type n.d.) figure 1.2 (Blood type, Antigens-Antibodies n.d.) Multiple
More informationLaboratory Exercise # 11: Differentiation of the Species Staphylococcus and Streptococcus
Laboratory Exercise # 11: Differentiation of the Species Staphylococcus and Streptococcus Purpose: The purpose of this laboratory exercise is to explore the differences between Staphylococcal species and
More informationChapter 4. Microscopy, Staining, and Classification. Lecture prepared by Mindy Miller-Kittrell North Carolina State University
Chapter 4 Microscopy, Staining, and Classification 2012 Pearson Education Inc. Lecture prepared by Mindy Miller-Kittrell North Carolina State University Microscopy and Staining 2012 Pearson Education Inc.
More informationHelices From Readily in Biological Structures
The α Helix and the β Sheet Are Common Folding Patterns Although the overall conformation each protein is unique, there are only two different folding patterns are present in all proteins, which are α
More informationMICROSCOPY OF LIVING MICROBES
EXPERIMENT 1 MICROSCOPY OF LIVING MICROBES Many students taking microbiology for the first time feel that they are going to have a hard time with the microscope. This lab as an experiment is intended to
More informationIn order to be useful, a smear must have the following qualities:
Smear Preparation and Simple Stain Objectives: Make bacterial smear slides (usually called smears) Distinguish cells on these slides using a simple stain procedure Unstained microbial cells are nearly
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/7/339/ra80/dc1 Supplementary Materials for Manipulation of receptor oligomerization as a strategy to inhibit signaling by TNF superfamily members Julia T. Warren,
More informationBiology 101 Chapter 4 Cells as the Basic Unit of Life. The Cell Theory Major Contributors: Galileo = first observations made with a microscope
Biology 101 Chapter 4 Cells as the Basic Unit of Life The Cell Theory Major Contributors: Galileo = first observations made with a microscope Robert Hooke = first to observe small compartments in dead
More information1.1.2. thebiotutor. AS Biology OCR. Unit F211: Cells, Exchange & Transport. Module 1.2 Cell Membranes. Notes & Questions.
thebiotutor AS Biology OCR Unit F211: Cells, Exchange & Transport Module 1.2 Cell Membranes Notes & Questions Andy Todd 1 Outline the roles of membranes within cells and at the surface of cells. The main
More informationCandy Antigens and Antibodies
LAB ACTIVITY: Candy Antigens and Antibodies TEACHER S EDITION Curricular Links: Biology 20, Science 30 Class Time: 1 period Suggested Answers: Question 1: What is the main function of red blood cells?
More informationEvidentiary value and effects of contaminants on blood group factors in medico-legal grounds
Original Research Article Evidentiary value and effects of contaminants on blood group factors in medico-legal grounds Ashwini Narayan K 1*, Manjunath MR 2, Kusuma KN 3 1 Assistant Professor, Department
More informationHow To Identify A Klebsiella Pneumoniae Strain From A Urinary Tract Infection
JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 1997, p. 2370 2374 Vol. 35, No. 9 0095-1137/97/$04.00 0 Copyright 1997, American Society for Microbiology Identification of a Klebsiella pneumoniae Strain Associated
More informationKEY CHAPTER 14: BLOOD OBJECTIVES. 1. Describe blood according to its tissue type and major functions.
KEY CHAPTER 14: BLOOD OBJECTIVES 1. Describe blood according to its tissue type and major functions. TISSUE TYPE? MAJOR FUNCTIONS connective Transport Maintenance of body temperature 2. Define the term
More informationCells and Their Housekeeping Functions Cell Membrane & Membrane Potential
Cells and Their Housekeeping Functions Cell Membrane & Membrane Potential Shu-Ping Lin, Ph.D. Institute of Biomedical Engineering E-mail: splin@dragon.nchu.edu.tw Website: http://web.nchu.edu.tw/pweb/users/splin/
More informationMembrane Structure and Function
Membrane Structure and Function -plasma membrane acts as a barrier between cells and the surrounding. -plasma membrane is selective permeable -consist of lipids, proteins and carbohydrates -major lipids
More informationCharacteristics and Serologic Determination of Antibodies to High Frequency Antigens
Characteristics and Serologic Determination of Antibodies to High Frequency Antigens Nicole Thornton The International Blood Group Reference Laboratory Bristol, United Kingdom. 23 rd Regional Congress
More information3.1 AS Unit: Cells, Exchange and Transport
3.1 AS Unit: Cells, Exchange and Transport Module 1: Cells 1.1.1 Cell Structure Candidates should be able to: (a) state the resolution and magnification that can be achieved by a light microscope, a transmission
More information14 The ability of the lenses to distinguish fine detail and structure is called a. Illumination b. Magnification c. Refractive index d.
1 2 Assume you stain Bacillus by applying malachite green with heat and then counterstain with safranin. Through the microscope, the green structures are a. cell walls. b. capsules. c. endospores. d. flagella.
More informationVeterinary Testing. Classes of Test
Veterinary Testing Classes of Test July 2014 Copyright National Association of Testing Authorities, Australia 2014 This publication is protected by copyright under the Commonwealth of Australia Copyright
More informationChapter 11: Molecular Structure of DNA and RNA
Chapter 11: Molecular Structure of DNA and RNA Student Learning Objectives Upon completion of this chapter you should be able to: 1. Understand the major experiments that led to the discovery of DNA as
More informationTopic: Serological reactions: the purpose and a principle of reactions. Agglutination test. Precipitation test. CFT, IFT, ELISA, RIA.
Topic: Serological reactions: the purpose and a principle of reactions. Agglutination test. Precipitation test. CFT, IFT, ELISA, RIA. Serology is the study and use of immunological tests to diagnose and
More informationFrom Coffee to Carbon
From Coffee to Carbon Abstract Using copy-and-cut cards, students place biological structures in order by their relative size from largest to smallest. Learning Objectives Understand the relative size
More informationANTIBIOTIC INHIBITION OF BACTERIA
ANTIBIOTIC INHIBITION OF BACTERIA STANDARDS 3.2.10B, 3.2.12B Apply process knowledge and evaluate experimental information 3.3.10B, 3.3.12B Chemical and structural basis of living organisms Westminster
More information7.1 What Are Cells? You are made of cells. A cell is the basic unit of structure and function in a living thing. CHAPTER 7
CELL STRUCTURE AND FUNCTION 7.1 What Are Cells? Look closely at the skin on your arm. Can you see that it is made of cells? Of course not! Your skin cells are much too small to see with your eyes. Now
More informationSupplemental Fig. S1. The schematic diagrams of the expression constructs used in this study.
1 Supplemental data Supplemental Fig. S1. The schematic diagrams of the expression constructs used in this study. Supplemental Fig. S2. Ingenuity Pathway Analysis (IPA) of the 56 putative caspase substrates
More informationSix major functions of membrane proteins: Transport Enzymatic activity
CH 7 Membranes Cellular Membranes Phospholipids are the most abundant lipid in the plasma membrane. Phospholipids are amphipathic molecules, containing hydrophobic and hydrophilic regions. The fluid mosaic
More informationThe Cell: Organelle Diagrams
The Cell: Organelle Diagrams Fig 7-4. A prokaryotic cell. Lacking a true nucleus and the other membrane-enclosed organelles of the eukaryotic cell, the prokaryotic cell is much simpler in structure. Only
More informationThe Nucleus: DNA, Chromatin And Chromosomes
The Nucleus: DNA, Chromatin And Chromosomes Professor Alfred Cuschieri Department of Anatomy, University of Malta. Objectives By the end of this unit the student should be able to: 1. List the major structural
More informationCHARACTERIZATION OF INDIGENOUS BACTERIA AND PATHOGENS
Indigenous Bacteria and Pathogens 1 CHARACTERIZATION OF INDIGENOUS BACTERIA AND PATHOGENS All plants and animals are natural habitats for bacteria. The naturally occurring, and generally nonpathogenic
More informationLAB: Blood Exploration
Name: Period: Date: LAB: Blood Exploration Introduction A liquid called plasma makes up about half (55%) of the content of blood. Plasma contains proteins that help blood to clot, transport substances
More informationLymph capillaries, Lymphatic collecting vessels, Valves, Lymph Duct, Lymph node, Vein
WLHS/A&P/Oppelt Name Lymphatic System Practice 1. Figure 12-1 provides an overview of the lymphatic vessels. First color code the following structures. Color code in Figure 12-1 Heart Veins Lymphatic vessels/lymph
More informationBIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT
BIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT UNIVERSITY OF PNG SCHOOL OF MEDICINE AND HEALTH SCIENCES DISCIPLINE OF BIOCHEMISTRY AND MOLECULAR BIOLOGY BMLS II / B Pharm II / BDS II VJ Temple
More informationUnit 2: Cells, Membranes and Signaling CELL MEMBRANE. Chapter 5 Hillis Textbook
Unit 2: Cells, Membranes and Signaling CELL MEMBRANE Chapter 5 Hillis Textbook HOW DOES THE LAB RELATE TO THE NEXT CHAPTER? SURFACE AREA: the entire outer covering of a cell that enables materials pass.
More informationLecture 8. Protein Trafficking/Targeting. Protein targeting is necessary for proteins that are destined to work outside the cytoplasm.
Protein Trafficking/Targeting (8.1) Lecture 8 Protein Trafficking/Targeting Protein targeting is necessary for proteins that are destined to work outside the cytoplasm. Protein targeting is more complex
More informationBSC 2010 - Exam I Lectures and Text Pages. The Plasma Membrane Structure and Function. Phospholipids. I. Intro to Biology (2-29) II.
BSC 2010 - Exam I Lectures and Text Pages I. Intro to Biology (2-29) II. Chemistry of Life Chemistry review (30-46) Water (47-57) Carbon (58-67) Macromolecules (68-91) III. Cells and Membranes Cell structure
More informationFigure 14.2 Overview of Innate and Adaptive Immunity
I M M U N I T Y Innate (inborn) Immunity does not distinguish one pathogen from another Figure 14.2 Overview of Innate and Adaptive Immunity Our first line of defense includes physical and chemical barriers
More informationLaboratory Protocols Level 2 Training Course Serotypning of Salmonella enterica O and H antigen.
Global Salm-Surv A global Salmonella surveillance and laboratory support project of the World Health Organization Laboratory Protocols Level 2 Training Course Serotypning of Salmonella enterica O and H
More informationGreen Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein
Green Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein INTRODUCTION Green Fluorescent Protein (GFP) is a novel protein produced by the bioluminescent
More informationBacteria: The good, the bad, and the ugly. SEPA BioScience Montana Module 2
Bacteria: The good, the bad, and the ugly. SEPA BioScience Montana Module 2 Introduction: The following reading will give you a basic introduction to bacteria and their role in illness. It will explore
More informationBlood Sticky, opaque fluid with a metallic taste (Fe 2+ ) Varies from scarlet (P O2 = 100) to dark red (P O2 = 40) ph is between 7.35 and 7.45 Average volume in an adult is 5 L (7% of body weight) 2 L
More informationQuestion Bank Five Kingdom Classification
Question Bank Five Kingdom Classification 1. Who proposed Five Kingdom Classification? Give the bases of classification. Ans. Whittaker in 1969 proposed five kingdom classification based on :- (i) Cell
More informationThe Cell Interior and Function
The Cell Interior and Function 5 5.0 CHAPTER PREVIEW Investigate and understand the organization and function of the cell interior. Define the differences between eukaryotic and prokaryotic cell structure.
More informationThe general structure of bacteria
The general structure of bacteria The uni-cellular organisms Viruses Herpes virus, HIV, influenza virus The procaryotic organisms Escherichia, Salmonella, Pseudomonas Streptococcus, Staphylococcus, Neisseria
More informationPractical Cell Analysis
Practical Cell Analysis Dimitri Pappas Dept of Chemistry & Biochemistry, Texas Tech University, USA WILEY A John Wiley and Sons, Ltd, Publication Contents Preface Acknowledgments xiii xix 1 Getting Started
More information7.2 Cells: A Look Inside
CHAPTER 7 CELL STRUCTURE AND FUNCTION 7.2 Cells: A Look Inside Imagine a factory that makes thousands of cookies a day. Ingredients come into the factory, get mixed and baked, then the cookies are packaged.
More informationGround substance is the component of connective tissue between the cells and the fibers
Connective Tissues Directions: Insert and install your Interactions: Foundations CD. a. Click the "Contents" button. b. Open the Tissue Level of Organization file. c. Click on Anatomy Overviews. d. Work
More informationTransformation of the bacterium E. coli. using a gene for Green Fluorescent Protein
Transformation of the bacterium E. coli using a gene for Green Fluorescent Protein Background In molecular biology, transformation refers to a form of genetic exchange in which the genetic material carried
More informationChapter 18. Blood Types
Chapter 18 Blood Types Blood Types blood types and transfusion compatibility are a matter of interactions between plasma proteins and erythrocytes Karl Landsteiner discovered blood types A, B and O in
More informationCarbohydrates, proteins and lipids
Carbohydrates, proteins and lipids Chapter 3 MACROMOLECULES Macromolecules: polymers with molecular weights >1,000 Functional groups THE FOUR MACROMOLECULES IN LIFE Molecules in living organisms: proteins,
More informationPlatelet Transmission Electron Microscopy and Flow Cytometry 11/15/2015
Welcome to Mayo Medical Laboratories Hot Topics. These presentations provide short discussion of current topics and may be helpful to you in your practice. Today our topic looks at hereditary platelet
More informationBIOLOGICAL MOLECULES OF LIFE
BIOLOGICAL MOLECULES OF LIFE C A R B O H Y D R A T E S, L I P I D S, P R O T E I N S, A N D N U C L E I C A C I D S The Academic Support Center @ Daytona State College (Science 115, Page 1 of 29) Carbon
More informationCell membranes and transport. Learning Objective:
Cell membranes and transport Content Fluid mosaic model of membrane structure Movement of substances into and out of cells Learning Objective: a. Describe and explain the fluid mosaic model of membrane
More informationChapter 4: A Tour of the Cell. 1. Cell Basics. Limits to Cell Size. 1. Cell Basics. 2. Prokaryotic Cells. 3. Eukaryotic Cells
Chapter 4: A Tour of the Cell 1. Cell Basics 2. Prokaryotic Cells 3. Eukaryotic Cells 1. Cell Basics Limits to Cell Size There are 2 main reasons why cells are so small: If cells get too large: 1) there
More informationSection 7-3 Cell Boundaries
Note: For the past several years, I ve been puzzling how to integrate new discoveries on the nature of water movement through cell membranes into Chapter 7. The Section below is a draft of my first efforts
More informationEssentials in Immunology Prof. R. Manjunath Department of Biochemistry Indian Institute of Science, Bangalore
Essentials in Immunology Prof. R. Manjunath Department of Biochemistry Indian Institute of Science, Bangalore Lecture No. # 02 Cells and Organs of the immune system Part 1 (Refer Slide Time: 00:27) Hello
More informationAdapted from Biology 15 Laboratory Supplemental Manual: Wrightsman, Ininns and Cannon- Moloznic.
Biology 3B Laboratory Cultural Characteristics of Bacteria Objectives: Describe bacterial structure: colony morphology, cell shape, growth patterns. To distinguish how various growth media will affect
More informationMicroscopes. Eukaryotes Eukaryotic cells are characterized by having: DNA in a nucleus that is bounded by a membranous nuclear envelope
CH 6 The Cell Microscopy Scientists use microscopes to visualize cells too small to see with the naked eye. In a light microscope (LM), visible light is passed through a specimen and then through glass
More informationAmong a cell s most important activities are its interactions
6 Membranes Concept Outline 6.1 Biological membranes are fluid layers of lipid. The Phospholipid Bilayer. Cells are encased by membranes composed of a bilayer of phospholipid. The Lipid Bilayer Is Fluid.
More informationVaxign Reverse Vaccinology Software Demo Introduction Zhuoshuang Allen Xiang, Yongqun Oliver He
Vaxign Reverse Vaccinology Software Demo Introduction Zhuoshuang Allen Xiang, Yongqun Oliver He Unit for Laboratory Animal Medicine Department of Microbiology and Immunology Center for Computational Medicine
More informationNomenclature of fatty acids. Fatty Acids. Chapter 9: Lipids. Fatty acids are carboxylic acids with a long hydrocarbon chain
Chapter 9: Lipids Definition: those molecules which can be extracted from biological tissue with a nonpolar solvent Structural relationships of major lipid classes Lipids are essential components of all
More informationCell Membrane & Tonicity Worksheet
NAME ANSWER KEY DATE PERIOD Cell Membrane & Tonicity Worksheet Composition of the Cell Membrane & Functions The cell membrane is also called the PLASMA membrane and is made of a phospholipid BI-LAYER.
More informationEXPERIMENT #1: MICROSCOPY
EXPERIMENT #1: MICROSCOPY Brightfield Compound Light Microscope The light microscope is an important tool in the study of microorganisms. The compound light microscope uses visible light to directly illuminate
More informationMetabolism Dr.kareema Amine Al-Khafaji Assistant professor in microbiology, and dermatologist Babylon University, College of Medicine, Department of
Metabolism Dr.kareema Amine Al-Khafaji Assistant professor in microbiology, and dermatologist Babylon University, College of Medicine, Department of Microbiology. Metabolism sum of all chemical processes
More informationExplain the role of blood and bloodstain patterns in forensics science. Analyze and identify bloodstain patterns by performing bloodstain analysis
Lab 4 Blood Learning Objectives Explain the role of blood and bloodstain patterns in forensics science Analyze and identify bloodstain patterns by performing bloodstain analysis Introduction Blood, a
More information