ual EasyCon N) anual epblue with EasyCon Software manual Software version 40.4

Transcription

1 ual EasyCon N) anual Software manual Software version 40.4

2 Copyright 2015 Eppendorf AG. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner. Focus Diagnostic is a registered trademark of Focus Diagnostic, Inc., USA. Qiagen and Rotor-Disk are registered trademarks of Qiagen GmbH, Germany. Excel and Windows are registered trademarks of Microsoft Corporation in the United States and other countries. epmotion, Eppendorf Tubes ept.i.p.s., and Eppendorf ThermoMixer are registered trademarks of Eppendorf AG, Germany. Eppendorf and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany. Registered trademarks and protected trademarks are not marked in all cases with or in this manual. The software of the device (firmware) contains open source software. License information is available on request from Eppendorf AG. Only for epmotion M5073 and 5075m: Limited Use Label License NOTICE TO PURCHASER; LIMITED LICENSE FOR RESEARCH USE ONLY. This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The purchase price for this product includes only limited, nontransferable rights under certain claims of certain patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this product for any commercial use. Further information regarding purchasing a license under patents of Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development Department, Genetic Center Drive, San Diego, California , U.S.A /082015

3 Table of contents 3 Table of contents 1 Operating instructions Using this manual Danger symbols and danger levels Danger symbols Danger levels Symbols used Abbreviations used Glossary Version overview for the software manual epblue with EasyCon Safety Product description Features epblue Assistants Operation Starting and exiting epblue Starting epblue Exiting epblue and switching off EasyCon Tablet Initial steps Using touchscreen and mouse Using the screen keyboard File menu Status area Enlarging the view epblue Application Runner - Carrying out the application Opening an application Preparing the application run Selecting the device Entering the number of samples Setting the HEPA air filter and the UV lamp Positioning the labware on the epmotion worktable Setting the run parameters for the optical sensor Setting the run parameters for the labware Entering the filling volume of the labware Starting the application Controlling the application Ending the application epblue Log viewer - Managing application logs Displaying protocols Saving the log as a PDF file

4 4 Table of contents 7 epblue Application Editor - Creating and editing applications Overview Creating an application Menu bar Creating an empty application Copying applications Saving applications Positioning labware Defining the application parameters of the labware Defining procedures Checking an application Commands Sample transfer command Reagent transfer command Dilute command Pool command Pool one destination command Pierce command Comment command Exchange command Magnetic separation command Mix command Number of samples command Temperature command ThermoMixer command Transport command User intervention command External process command Wait command Pattern Menu bar in the Edit Pattern window Defining regular patterns Defining irregular patterns Optical sensor Measuring the filling level of the labware Detecting pipette tips Checking the labware on the worktable epblue Application Editor - Managing applications and folders Menu bar and start screen Copying and deleting applications Copying applications Deleting an application Changing the properties and name of the application Importing and exporting applications Importing applications Exporting applications Saving an application as a PDF file Saving an application for a different epmotion model

5 Table of contents 5 9 epblue Labware Editor - Creating and managing labware Overview of labware Managing labware Menu bar Deactivating and activating labware Deleting labware Importing labware Exporting labware Viewing the properties of the labware Setting the bottom tolerance Creating labware combinations Creating a labware combination Creating a labware combination based on existing labware Equipping the racks, thermoracks, and ReservoirRack modules with individual vessels Equipping the ReservoirRack with reservoirs and ReservoirRack modules Labware library Loading labware definitions from the Internet Request labware definitions Add a labware definition to a labware library Special labware features in epblue Location B0 on the worktable Tubes MTP plates PCR plates ept.i.p.s. Motion Racks Rack Rack LC ReservoirRack ReservoirRack Module TC Thermoracks Thermorack Rotor/Tubes TipHolder Thermoadapter Universal Disc by Focus Diagnostics Thermoblocks Thermoblock PCR 96 OC

6 6 Table of contents 10 epblue settings - Checking components and making the basic settings Tool interlock Lock the tool Unlocking the tool Dosing device Reading out the tool Gripper Moving the gripper arms UV lamp Decontaminating the worktable HEPA air filter Switching on the HEPA air filter Switching off the HEPA air filter epblue Administration epblue Administration Administration Starting epblue Exit epblue Administration Changing the device name Print templates Creating a print template Saving print templates as default Deleting a print template Data backup Backing up the database Restoring the database Importing a database from epblue version 40.X Temperature control unit X Levelsensor options ThermoMixer Checking the mixing function of the Eppendorf ThermoMixer module Checking the temperature control of the Eppendorf ThermoMixer module Magnetic separation epblue - Software error messages Error messages Ordering information Accessories Index

7 Operating instructions 7 1 Operating instructions 1.1 Using this manual Your epmotion operating manual consists of hardware instructions and software instructions. Separate instructions exist for some optional software modules. The operating manual is part of the product. The current version of the operating manual can be found on our website: Read the operating manual in full before using the device. Store the operating manual at an easily accessible location. The device may only be transferred with the operating manual. If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details. 1.2 Danger symbols and danger levels Danger symbols The safety instructions in this manual appear with the following danger symbols and danger levels: Biohazard Explosive substances Strong magnetic field UV radiation Electric shock Heavy load Cuts Hot surface Hazard point Material damage Danger levels DANGER WARNING CAUTION NOTICE Will lead to severe injuries or death. May lead to severe injuries or death. May lead to light to moderate injuries. May lead to material damage.

8 8 Operating instructions 1.3 Symbols used Depiction Meaning Actions in the specified order Actions without a specified order List Text Display text or software text Additional information 1.4 Abbreviations used CSV Comma Separated Value DNA Deoxyribonucleic acid DWP Deepwell plate ept.i.p.s. Eppendorf Totally Integrated Pipetting System HEPA High Efficiency Particulate Air (filter) LH Liquid Handling LIMS Laboratory Information Management System MTP Microplate PCR Polymerase chain reaction PDF Portable Document Format TMX Eppendorf ThermoMixer module USB Universal Serial Bus UV Ultraviolet radiation

9 Operating instructions Glossary A Administrator User possessing the rights for the administration of epblue. Application Program for a dedicated application. An application comprises the procedure and the equipping of the worktable. C Cleanbench Class 2 safety cabinet for personal security and product protection. A laminar air flow prevents germs from entering the cabinet from the ambience. The air flow also prevents aerosols that contain microorganisms from escaping the cabinet. CleanCap Optional epmotion equipment. The CleanCap includes a HEPA filter and at least one UV lamp. Command Step in a procedure. Various parameters are set for commands. Each command triggers an action, e.g., the transport of liquids. Control device Device for controlling the epmotion. Depending on the equipment of the epmotion, a MultiCon PC or a EasyCon Tablet is available. CSV file Text file for saving and exchanging structured information. D Destination labware Rack or plate with destination positions. Destination position Position in the labware that exclusively is destination of liquid transfers. Dispensing tool Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing tools are available for various volume ranges. E EasyCon Tablet Eppendorf EasyCon Tablet. You can control your epmotion using the tablet and the epblue software.

10 10 Operating instructions epblue Software for creating and administrating applications and labware. The epblue software is used for controlling the epmotion. ept.i.p.s. Motion Pipette tips of the epmotion. ept.i.p.s. Motions are available with or without a filter. The abbreviation stands for Eppendorf Totally Integrated Pipetting System. F Filling volume Maximum volume of a labware. The filling volume is higher than the working volume. The epmotion removes liquid from the labware up to the filling volume. G Gripper Tool that transports labware. H Height adapter Adapter for low labware. The height differences between the labware is offset to decrease the tool holder paths, and therefore decrease the cycle time of the application. HEPA filter Air filter. The filter prevents particles such as dust and germs from the environment entering the epmotion workspace. L Labware Racks, plates, tips, etc. which are placed on the worktable. Location Area where labware is placed on the worktable. M MagSep System for nucleic acid preparation with the epmotion. The MagSep consists of a magnetic finger module, an Eppendorf ThermoMixer module, a PrepRack, and reagents. P Pattern Pattern in which liquid is aspirated and dispensed. Patterns are defined in a transfer command. Patterns are defined as regular patterns and irregular patterns. Patterns are independent in x-direction and y-direction.

11 Operating instructions 11 Procedure Sequence of commands executed consecutively. Part of an application. R Rack Mount for tubes or pipette tips. Remaining volume Volume that cannot be removed from a vessel. Caused by the distance from the pipette tip to the vessel base, defined in the software, and the defined immersion depth of the pipette tip in the liquid. The pipette tip cannot aspirate the volume. The remaining volume depends on the vessel geometry. Reservoir Reservoirs are used for preparing reagents. Reservoirs are hung in a ReservoirRack or placed directly on the worktable. S SafeRack Rack with ep.t.i.p.s. Motion pipette tips. The SafeRack features a partition that prevents the contamination of adjacent tips. If you want to use the tips several times, use SafeRacks. Source labware Rack or plate with source positions. Source position Position in a labware that exclusively is a source of liquid transfers. T Thermal module Heat plate for labware. The heat plate is integrated in the worktable. Thermoadapter Temperature-controlled adapter that is not transported with the gripper. Plates are inserted into the thermoadapter. Thermoblock Temperature-controlled adapter that is transported with the gripper. There are 2 types of thermoblocks: Thermoblocks: Thermoblocks, into which plates are inserted. Thermoblocks with plates Thermoblocks that are permanently connected with a plate. Thermorack Temperature-controlled rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 ml, 1.5 ml or 2 ml. Tube A micro test tube or a single well in a plate.

12 12 Operating instructions W Work volume Filling volume minus volume of the immerging pipette tip. The epmotion fills the vessels up to the working volume. Worktable epmotion work surface that labware and tools are placed on. In the software, the epmotion worktable is represented as epblue worktable. 1.6 Version overview for the software manual epblue with EasyCon Order no.: Version Issue date Change 00 August 2013 Creation. Software version August 2015 Update to software version 40.4.

13 Safety 13 2 Safety The intended use and safety instructions sections from the hardware manual apply for using the epmotion with the epblue 40.X software.

14 14 Safety

15 Product description 15 3 Product description 3.1 Features With the epblue software you control the epmotion. epblue consists of several modules. Which modules are available depends on the features of your epmotion and the specification of the software epblue Modules Description Operating manual Application Editor Application Runner Create applications. Manage applications. Execute applications. (see epblue Application Editor - Creating and editing applications on p. 35) (see epblue Application Editor - Managing applications and folders on p. 83) (see epblue Application Runner - Carrying out the application on p. 23) Labware Editor Manage labware. (see epblue Labware Editor - Creating and managing labware on p. 91) Log Viewer Display and save logs. (see epblue Log viewer - Managing application logs on p. 33) Settings Administration Service Check components of the device. Perform basic settings. Backup data. Create print templates. Access for Eppendorf service technician. (see epblue settings - Checking components and making the basic settings on p. 109) (see epblue Administration on p. 115) Assistants Assistants are programs for a specific application. Assistants are optionally available and described in separate operating manuals. The following assistants are available for epblue: Prep Assistant Nucleic acid preparation with Eppendorf MagSep kits. After that, a PCR setup can be performed. PCR Assistant Create PCR master mixes and dilution series for quantification standards, normalization of sample concentrations, and setting up PCR reactions. LH assistant Normalization of sample concentrations.

16 16 Product description

17 Operation 17 4 Operation 4.1 Starting and exiting epblue Starting epblue Prerequisites epmotion and EasyCon Tablet were installed by a service technician. Switch on the epmotion using the mains/power switch. The EasyCon Tablet is started at the same time as the epmotion. epblue starts automatically when the EasyCon Tablet is ready for operation. The epblue start screen appears. Abb. 4-1: epblue start screen Fig. 4-1: epblue start screen 1 File menu Contains functions that can be accessed in all applications. 2 Information area Contains information on the open file. 3 Status area Status of the epmotion 4 Working area Contents and applications.

18 18 Operation Exiting epblue and switching off EasyCon Tablet NOTICE! Data loss due to power-off without shutting down the device first. The EasyCon Tablet mains/power supply is terminated when the epmotion is switched off. Failure to first shut down the EasyCon Tablet may result in data loss. This can damage the EasyCon Tablet. Shut down the EasyCon Tablet before switching off the epmotion. 1. In the File menu, select Shut down. 2. If the touchscreen of the EasyCon Tablet is dark, switch off the epmotion at the mains/power switch. 4.2 Initial steps Using touchscreen and mouse You can operate the operating unit via the touchscreen or use a USB mouse. Connect the mouse to a USB interface on the operating unit. Tab. 4-1: Touchscreen/mouse comparison Touchscreen Tap Tap and hold 2 s Drag Tap twice Mouse Click using the left mouse button Click using the right mouse button Drag with the left mouse button pressed down Double-click using the left mouse button Using the screen keyboard Enter the numbers and letters using the screen keyboard. Abb. 4-2: Screen keyboard Fig. 4-2: Screen keyboard Automatically show the screen keyboard epblue automatically shows a keyboard if you have selected an input field. Manually show the screen keyboard In the File menu, select the Show keyboard entry. Switch the automatic display of the screen keyboard on and off. In the File menu, select the Show keyboard automatically entry.

19 Operation 19 Tab. 4-2: epblue key combinations Enter special characters. Press the letter key for 2 s. Confirm your entry. Press the key once. Switch between lowercase letters and capital letters. Switch the keyboard. Show the screen keyboard. To enter a capital letter, press the key once. Then press the letter. To switch the keyboard to all capital letters, press the key twice. To switch between the English and German keyboards, press the key once. Press the key once File menu Abb. 4-3: File drop-down menu Fig. 4-3: File drop-down menu Exit to start screen entry Show the epblue start screen. Menu Show keyboard Manually show the screen keyboard. Menu Show keyboard automatically Switch the automatic display of the screen keyboard on and off. Sound enabled entry Switches the system sound on and off. Shutdown entry End the program and shutdown the operating device.

20 20 Operation Abb. 4-4: Help drop-down menu Fig. 4-4: Help drop-down menu Device manuals entry Open the operating manual of the device. About epblue entry Show the epblue version. Software manuals entry Open the operating manual of the software Status area The status area is located in the top right of the screen. If you click the epmotion in the status area, the following information is displayed: epmotion serial number. Equipment of the epmotion Firmware version Status of the epmotion Actual temperature of a thermal module or an Eppendorf ThermoMixer module. Example Info: C3:21 C. The thermal module in location C3 has a temperature of 21 C.

21 Operation Enlarging the view You can enlarge the image of a set of labware. All positions of the labware become clearly visible. The enlarging function is a help, e.g. for patterning. You can use the navigation window for better navigation in the enlarged image. Abb. 4-5: View window Fig. 4-5: View window Decrease the image in the view window. Press button or scroll with the mouse wheel. Enlarge the image in the view window. Press button or scroll with the mouse wheel. 72 % Display the image in the view window at the desired percentage. Show the navigation window. Press the Cross button and move the navigation window. Alternatively, press the Ctrl key on the keyboard. Press and hold the left mouse wheel. Move the navigation window.

22 22 Operation Abb. 4-6: Navigation window Fig. 4-6: Navigation window Return to previous view. Go to next view. Scale image to 100%. Scale image to the size of the view window. Center image in the view window. The enlargement remains unchanged. Close the navigation window.

23 epblue Application Runner - Carrying out the application 23 5 epblue Application Runner - Carrying out the application This chapter describes how to carry out an application on the epmotion. 5.1 Opening an application Abb. 5-1: Selecting an application Fig. 5-1: Selecting an application Prerequisites epmotion is ready for operation. Application Runner is started. 1. Select the application in the Select application area. Recently used applications can be found in the Recently used area. Open applications in the Select application area have a red padlock. The Properties area displays the properties of the selected application. 2. Press the Next button. The application is opened.

24 24 epblue Application Runner - Carrying out the application 5.2 Preparing the application run Selecting the device Abb. 5-2: Selection of an available device Fig. 5-2: Selection of an available device NOTICE! Tool collisions with incorrectly positioned labware. Labware must be positioned on the epmotion worktable as defined in the application. Tools and labware become damaged in a collision. A collision can lead to sample loss. Check the positioning of the labware before starting the application. In the Available Devices area, the connected epmotion is displayed. Prerequisites epmotion is ready for operation. 1. Select the device for the application run. 2. Press the Next button.

25 epblue Application Runner - Carrying out the application Entering the number of samples Abb. 5-3: Process control window Fig. 5-3: Process control window The window Process control appears if the number of samples in the application is variable. 1. Enter the number of samples for the current run. 2. Press the OK button Setting the HEPA air filter and the UV lamp Abb. 5-4: Setting the parameters for the HEPA air filter and the UV lamp Fig. 5-4: Setting the parameters for the HEPA air filter and the UV lamp If your epmotion is equipped with a HEPA air filter and UV lamp, you can define the settings for the HEPA air filter and the UV lamp. Activate or deactivate the HEPA air filter. Define whether a decontamination is to be carried out before the application. The worktable may only be decontaminated if no labware or tools are present on the worktable.

26 26 epblue Application Runner - Carrying out the application Positioning the labware on the epmotion worktable Position the labware on the epmotion worktable according to the application. Information on positioning the labware can be found in the hardware operating manual Setting the run parameters for the optical sensor In the application, you defined the application parameters for the optical sensor. You can enter run parameters to use other settings of the optical sensor for an individual run. The run parameters overwrite the application parameters for this run only (see Optical sensor on p. 77). Abb. 5-5: Setting the run parameters Fig. 5-5: Setting the run parameters Detect volumes radio button Filling volume measurement by the optical sensor. The parameters set in the application for the labware are used. Use required minimum volumes radio button epblue assumes that all vessels are filled with the minimum required volume for this run. Use this function for dry runs. Input volumes manually radio button Entering run parameters manually Detect tips slider Activate the Detect tips function. Check labware placement slider Activate the Check labware placement function. 1. Configure the functions of the optical sensor. 2. Press the Run button.

27 epblue Application Runner - Carrying out the application Setting the run parameters for the labware You have defined in the labware properties how the optical sensor measures the filling level in the application. In the run parameters, you can enter a different option for measuring the filling level. The run parameters overwrite the application parameters for this run only (see Optical sensor on p. 77). Abb. 5-6: Setting the run parameters for the labware Fig. 5-6: Setting the run parameters for the labware Prerequisites In the Volume settings, the Detect volumes is activated. 1. Select the labware. The window with the labware properties appears. 2. For plates, select the mode in which the optical sensor measures the plate filling level. To do so, activate the Random positions checkbox or the All used positions checkbox. 3. For ReservoirRack modules, highlight the ReservoirRack module in which the optical sensor measures the filling level. In the table, activate the Level sensor checkbox. The manual entry field is grayed out. You can no longer enter the volume manually. The optical sensor measures the volume. 4. To close the labware properties window, press the Next button.

28 28 epblue Application Runner - Carrying out the application Entering the filling volume of the labware Measuring the filling volume with the optical sensor If the Detect volumes function is active, the optical sensor checks the filling volume after the start. If the following problems occur, a dialog window appears: Filling volume too low. Filling volume too high. Filling volume cannot be correctly detected. Abb. 5-7: Checking window Fig. 5-7: Checking window Maximum volume Maximum filling volume for the position. Abort button Aborts the application. Minimum volume Filling volume required to remove the set amount of fluid. Calculated volume Calculated filling volume for the position. The filling volume is calculated from the labware data and the fluid level. Repeat scan button Measuring the filling volume again with the optical sensor User input button Entering the filling volume manually Accept level and continue button Accept the calculated filling volume. µl input field If the User Input button has been selected, enter the filling volume manually. NOTICE! Dispensing error due to refilling of vessels. The optical sensor only checks the level of the vessels at the specified location. Only change the volume of the vessels at the specified location. Do not change the volume of the vessels at other locations. If the Calculated volume is larger than the Maximum volume, remove liquid from the vessel. If the Calculated volume is smaller than the Minimal volume, add liquid to the vessel. If there is an air bubble in the liquid, the optical sensor cannot perform a measurement. Remove the air bubble. To repeat the detection, select the Repeat scan button.

29 epblue Application Runner - Carrying out the application Entering the filling volume manually If the Input volumes manually function is active, you have to enter the filling volume manually after the start. Abb. 5-8: Entering the filling volume Fig. 5-8: Entering the filling volume Labware info area Information on the selected labware. Liquid detection area Optical sensor settings for the selected labware. Off The filling volume will not be checked. Random positions The filling volume of the first, last and 8 randomly selected positions will be checked. All used positions The filling volume in all positions used will be checked. Tab. 5-1: Column Red border Center table Index column Name checkbox MIN column VOL [UL] column MAX column Meaning Volume column Enter the filling volume for the selected labware. Invalid filling volume entered. Filling volumes that are smaller than the required volume are invalid. Filling volumes that are greater than the maximum volume of the labware are invalid. Position in the labware. Configure the settings of the optical sensor. Minimum filling volume that is required for this run. Enter the filling volume manually. Maximum filling volume the labware can be filled with.

30 30 epblue Application Runner - Carrying out the application Proceed as follows: Enter the filling volume in the VOL [UL] column. To enter the same filling volume for the entire labware, press the Set all volumes button. Enter the filling volume. Enter the run parameters of the optical sensor in the Liquid detection area, if necessary. To define that epblue shall use the minimum filling volume for a position, press the Use minimal volume button. Press the Run button. 5.3 Starting the application Prerequisites Labware is positioned on the epmotion worktable. epmotion is ready for operation. The application run was prepared (see p. 24). Click on the Run button. The application starts.

31 epblue Application Runner - Carrying out the application Controlling the application Abb. 5-9: Ongoing application Fig. 5-9: Ongoing application Carry out the application. The epmotion executes the application until the end. Executing the application step-by-step. The epmotion executes the next step and then stops. Pause. The application stops, but it will not be aborted. Aborts the application. If you have stopped the application using the button, the button is now active. Procedure area Commands. The active command is highlighted blue. Worktable area Worktable. Remaining Runtime area Status of the application/runtime of the application/total time for the application. Log area Log. Control the application with the buttons from the Control area.

32 32 epblue Application Runner - Carrying out the application 5.5 Ending the application When the application run is finished, a message appears. Abb. 5-10: The application is finished Abb. 5-11: The application was canceled Fig. 5-10: The application is finished Fig. 5-11: The application was canceled 1. To confirm the message press the OK button. 2. In the Application Runner window, press the Next button. The final window appears. If your epmotion is equipped with a HEPA air filter and UV lamp, you can activate the HEPA air filter and the UV lamp. Abb. 5-12: Final window Fig. 5-12: Final window 3. Activate the HEPA air filter, if necessary. Prerequisites The worktable of the device must be cleared completely before activating the UV lamp. 4. Activate the UV lamp, if necessary. 5. Press the Exit button to end the application.

33 epblue Log viewer - Managing application logs 33 6 epblue Log viewer - Managing application logs This chapter describes how to view and save logs of applications. 6.1 Displaying protocols Abb. 6-1: Logs window Fig. 6-1: Logs window Prerequisites The application was executed (see p. 30). The epblue start screen is shown. 1. Click on the LogViewer symbol. Select the folder in which the application is saved. 2. Select the log. The log is opened.

34 34 epblue Log viewer - Managing application logs 6.2 Saving the log as a PDF file Prerequisites A USB storage medium is connected to the EasyCon Tablet. 1. Click on the symbol. The print preview opens. 2. Check the layout and set up the page. 3. Save the document as a PDF file. 4. Close the window. Tab. 6-1: Symbols in the print preview Search in the document. Show the first page. Print the document (not active) Show the previous page. Page setup. Show the next page. Scale the document. Show the last page. Zoom in the view. Create a PDF. Set the zoom factor. Close the view. Zoom out the view.

35 epblue Application Editor - Creating and editing applications 35 7 epblue Application Editor - Creating and editing applications This chapter describes how to create and edit applications in epblue. 7.1 Overview Applications are programmed processes in epblue. An application consists of equipping the worktable and a sequence of commands. Each command triggers a specific action. Applications are created in a fixed order: 1. Create an empty application (see p. 36). 2. Position labware and define the application parameters of the labware (see p. 37). 3. Define the procedure (see p. 43). 4. Check the application (see p. 45). 7.2 Creating an application Menu bar Abb. 7-1: Application Editor menu bar Fig. 7-1: Application Editor menu bar Saving the application. Deleting the application. Saving the application for another worktable. One step back. Saving all applications. One step forward. CSV Closing the application. Import CSV file. Cutting the application. Printing the application. Copying the application. Checking the application. Inserting the application. Go to Application Runner.

36 36 epblue Application Editor - Creating and editing applications Creating an empty application Abb. 7-2: Start screen of the Application Editor Fig. 7-2: Start screen of the Application Editor Prerequisites The Application Editor is open. 1. Select the folder in which the application is saved. 2. Click on the symbol in the menu bar. The Create new application window appears. epblue detects the connected device. 3. Enter the name and description of the application. 4. Close the window using the Create button. You have created an empty application. The Worktable window of the application opens Copying applications You can create new applications by copying and changing existing applications. The Eppendorf folder contains applications defined by Eppendorf AG. These applications cannot be executed or changed. They are used as templates and examples. In order to use applications from the Eppendorf folder, copy the corresponding application to your folder. To copy an application (see p. 84) Saving applications To save an application, click on the symbol in the toolbar. To save an application under a new name, click on the symbol in the toolbar. To save all open applications, click on the symbol in the menu bar.

37 epblue Application Editor - Creating and editing applications Positioning labware Positioning the labware on the epblue worktable Labware can only be positioned at specific locations. Information on positioning the labware can be found in the hardware operating manual. Abb. 7-3: Positioning the labware on the epblue worktable Fig. 7-3: Positioning the labware on the epblue worktable Labware Category column Overview of all labware types. Worktable area epblue worktable with positioned labware. Switch to procedure button To go to the Procedure window, press the Switch to procedure button. Labware area Labware that can be positioned on the epblue worktable. Properties area Properties of the selected labware. Placed Labware column Labware positioned on the epblue worktable. Trash Delete labware from the epblue worktable. Prerequisites Labware combinations have been created for reaction vessels (see p. 92). The application is open. The Worktable window is open. 1. Select labware from the Labware area. 2. Draw the labware to the desired position of the epblue worktable. If the labware cannot be positioned at this location, the location is marked in red.

38 38 epblue Application Editor - Creating and editing applications Deleting positioned labware from the epblue worktable There are several options to delete labware from the epblue worktable. Selecting the labware Activate the checkbox for the corresponding labware in the Placed labware column. Alternatively, select the labware with the mouse. Deleting labware Drag the selected labware into the trash with the mouse. Alternatively, click on the symbol in the menu bar. Alternatively, select the Delete function in the context menu Stacking labware The gripper stacks and transports labware. The gripper is required to stack labware or to unstack a stack during an ongoing application. You can stack a maximum of 5 plates on the worktable. You can stack a plate on a height adapter. The maximum stacking height is 126 mm. Stacked labware must have the same properties, e.g. name, geometry, and bottom tolerance. The gripper only transports one part at a time. The gripper can stack labware or unstack a stack of labware. Liquids cannot be transferred in a plate stack. Functions of the optical sensor with stacked plates The Check labware placement function is available. The Detect volumes function is not available. You can stack the following plates: EP_pDNA_96_MTP EP_TT_PCR_150 EP_TT_PCR_40 EP_DWP_1200 EP_pDNA_96_DWP EP_DWP_200 EP_DWP_2000 EP_DWP_1000 EP_DWP_500 EP_MTP_384 EP_MTP_96 EP_MTP_UV_VIS_96 EP_MTP_CC_96 EP_MTP_CI_96 EP_MTP_CC_6 EP_MTP_CC_12

39 epblue Application Editor - Creating and editing applications 39 EP_MTP_CC_24 EP_MTP_CC_48 EP_TT_PCR_150 EP_TT_PCR_250 EP_TT_PCR_40 The list is extended if new labware is available. Plates that are stacked may only be filled up to the working volume. Abb. 7-4: Stacking labware on the epblue worktable Fig. 7-4: Stacking labware on the epblue worktable Proceed as follows to stack labware at a location. 1. Place the lower labware at one location. 2. Position the upper labware at the same location. The Placed labware column shows, which labware is placed in the epblue worktable locations. The number behind the designation shows how many parts have been stacked at this location. Example: pcr384_3. Only 3 plates have been stacked.

40 40 epblue Application Editor - Creating and editing applications Defining the application parameters of the labware You can determine the properties of each labware used. Abb. 7-5: Properties window Fig. 7-5: Properties window To change the properties of a labware, double-click on the labware. Alternatively, select the labware and go to the Properties context menu with the right mouse button or click on the Properties button. The Properties window opens. Set the properties for the selected labware Defining the filling volume of the labware 1. Set how the filling volume of the labware is to be measured. 2. To measure the filling volume with the optical sensor (see Optical sensor on p. 77) 3. To enter the filling volume manually (see Entering the filling volume manually on p. 29)

41 epblue Application Editor - Creating and editing applications Opening sealed labware The epmotion can open the sealing of individual positions of a labware. The epmotion opens the sealing with a single-channel dispensing tool. If the Pierce options option is activated, epblue can open the sealing of the labware with the following commands: Commands Sample transfer, Reagent transfer, Dilute, Pool, Pool one destination: The sealing is opened. Immediately afterwards, a liquid transfer is performed. Pierce command: The sealing is opened. Immediately afterwards, a liquid transfer is performed. To pierce the sealing there are 2 modes. Single pierce mode: The pipette tip on the dispensing tool pierces the aluminum foil once in the middle of the position. Triple pierce mode: The pipette tip on the dispensing tool pierces the aluminum foil three times. The holes have a fixed distance to the middle of the position. The holes are pierced into the aluminum foil at a distance of 120. The Triple pierce mode is used to pierce a big hole into the sealing with small dispensing tools. The single-channel dispensing tool TS 1000 opens the sealing in factory setting in the Single pierce mode. The single-channel dispensing tools TS 50 and TS 300 open the sealing in factory setting in the Triple pierce mode. You can change the mode in which the dispensing tool opens the sealing. For this purpose, change the value in the Distance (see Fig. 7-6 on p. 42) field. Example: The single-channel dispensing tool TS 1000 opens the sealing in factory setting in the Single pierce mode. The distance of the dispensing tool to the middle of the position is zero. The value in the Distance field is zero. You change the value in the Distance field to 1. The single-channel dispensing tool TS 1000 will open the sealing in the Triple pierce mode. The holes have a distance of 1 mm to the middle of the position. After the sealing has been opened, the pipette tips can still be used or ejected (see Pierce command on p. 62). If the labware is sealed, the optical sensor cannot measure the filling level. If the Pierce settings option is activated, the Levelsensor settings option is not available. The labware must be sealed with aluminum foil that is marked with the word "Pierceable". If you have any questions on the suitable aluminum foil, please contact Automations Support at Eppendorf. Write an to automation-support@eppendorf.de. The parameters in the Pierce settings area have been optimized for the labware combination Thermoblock PCR 96 OC with twin.tec PCR Plate 96, semiskirted.

42 42 epblue Application Editor - Creating and editing applications Setting the parameters for opening sealed labware Abb. 7-6: Properties window in the Pierce settings area Fig. 7-6: Properties window in the Pierce settings area Distance Distance from the middle of the vessel. If the distance is zero, the single-channel dispensing tool opens the sealing in the Single pierce mode. If the distance is > zero, the single-channel dispensing tool opens the sealing in the Triple pierce mode. Above Distance between the starting position of the piercing movement and the vessel edge. At this distance, the pipette tip starts piercing. SPEED Speed at which the pipette tip opens the sealed labware. Depth Distance between vessel edge and pipette tip. This is how far the pipette tip is immersed into the vessel. 1. Select the labware. 2. Call up the properties of the labware. The Properties window opens. 3. Set parameters in the Pierce settings area. 4. Place the labware with an adapter on the worktable. 5. Perform a test run. Check whether the parameters in the Pierce settings area are adjusted to labware and aluminum foil.

43 epblue Application Editor - Creating and editing applications Setting the filling level of the labware You can set the filling level for all positions of the labware. 1. To set the same filling level for all positions of a labware, press the Set all volumes button. 2. To import the filling levels of the labware from a CSV file, press the Import all volumes button. 3. To enter the filling levels of the labware manually, enter the filling levels in the VOL [µl] column Defining procedures A procedure is a defined sequence of commands which epmotion executes during an application. Commands are described in the Commands chapter (see p. 46). The first command of each procedure is the Procedure Start command. The Procedure Start command is created automatically and cannot be deleted. The Procedure Start command shows the epblue worktable at the start of the application. The Procedure Start command does not influence the application. The software does not include any command for taking up pipette tips. The user defines which pipette tips will be used in a command. The user must provide for the corresponding pipette tips on the worktable. The user defines the dispensing tool for each command. Tools are picked up according to the command parameters.

44 44 epblue Application Editor - Creating and editing applications Abb. 7-7: Creating a procedure for an application Fig. 7-7: Creating a procedure for an application Commands column Available commands. Procedure column Sequence of commands. The commands are executed in the order displayed. Worktable The state of the worktable during the execution of the selected command Options area Parameter of the selected command. Prerequisites The application is open. Labware is positioned on the epblue worktable. The Procedure window is open.

45 epblue Application Editor - Creating and editing applications Inserting a command 1. Select a command from the Commands column. 2. Drag the command to the Procedure column. 3. In order to insert a command at the end of the application, double-click on the selected command in the column Commands Copying commands Select a command from the Procedure column. Click on the symbol in the menu bar. Alternatively, open the context menu with the right mouse button and select Copy Inserting a copied command 1. Copy the command. 2. Select the position in the Procedure column where you would like to insert the copied command. 3. Select the Paste command in the context menu. The copied command is inserted behind the selected position Changing the command sequence Select a command in the Procedure column. Drag the command to the desired position in the procedure Deleting commands Select a command in the Procedure column. Delete the command using the symbol in the menu bar or via the context menu Changing command parameters 1. Select a command in the Procedure column. The parameters of the selected command are shown in the Options area. 2. Set the parameters to suit your requirements Checking an application 1. Click on the symbol in the menu bar. 2. Select the Check command function. The application is checked. Example: Pipette tips without filter are positioned on the epmotion worktable. In the application, pipette tips with filter were selected. The screen displays an error message.

46 46 epblue Application Editor - Creating and editing applications 7.3 Commands A procedure is a sequence of commands that epmotion executes during an application run. Commands are executed one after the other and independent of each other. Each command has a specific set of parameters. The software does not include any command for taking up pipette tips. The user defines which pipette tips will be used in a command. The user positions the corresponding pipette tips on the worktable. The user defines the dispensing tool for each command. Tools are picked up according to the command parameters. The command parameters may differ. The displayed parameters depend on the selected worktable or on the configuration of the connected epmotion. Transfer commands Sample transfer Reagent transfer Dilute Pool Pool one destination Additional commands Mix ThermoMixer epmotion with Eppendorf ThermoMixer module Magnetic separation Temperature epmotion with epmotion with magnetic finger thermal module module Transport epmotion 5075 epmotion 5073 Exchange epmotion 5070 Wait User intervention Number of samples External process Pierce Comment

47 epblue Application Editor - Creating and editing applications Sample transfer command Various transfer commands are available to transfer liquids. All transfer commands are derived from the Sample transfer command. Abb. 7-8: Principle of the Sample transfer command Fig. 7-8: Principle of the Sample transfer command The Sample transfer command is used to transfer liquids from various source positions to several destination positions. The number of samples that will be aspirated from the source labware depends on the Number of samples (see p. 66) command. If the Pierce settings function is activated in the properties of the labware, the labware will be opened before the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened, the source vessel is opened first. To open the vessels, epblue uses the same dispensing tool and the same pipette tips as for liquid transfer.

48 48 epblue Application Editor - Creating and editing applications Sample transfer > Parameter command Abb. 7-9: Sample transfer > Parameter Fig. 7-9: Sample transfer > Parameter Volume Pipet. tool Pipette Multidispense Multiaspirate Use filter tips Source Destination Pattern Select volume in μl or nl. A decimal place is available for volumes 99.9 μl. You can transfer a volume larger than the maximum volume of the pipette tip. The volume to be transferred will be pipetted in several steps. Example: The user would like to transfer 1500 μl and enters this value into the Volume field. If a 1000 μl tip is being used, 2x 750 μl will be pipetted. If a 300 μl tip is being used, 5 x 300 μl will be pipetted. Used dispensing tool. The volume is individually aspirated and dispensed for each liquid transfer. Aspirate liquid and dispense it in the destination position in defined subsets. Aspirate liquid from several source positions using one pipette tip and dispense the liquid in one destination position. Available for Pool and Pool one destination commands. Activate when using tips with filter. Select the source labware. The source labware must be placed on the worktable. When using more than one source labware, the number of positions in the labware (384, 96, 24) must match. If you dispense within one labware, the source labware and destination labware are identical. Select the destination labware. The destination must be placed on the worktable. When using more than one destination labware, the number of positions in the labware (384, 96, 24) must match. If you dispense within one labware, the source labware and destination labware are identical. Select the source positions and destination positions. Create replicates. Information on the aspiration and transfer of liquids with the epmotion can be found in the hardware operating manual.

49 epblue Application Editor - Creating and editing applications Sample transfer > Options command Abb. 7-10: Sample transfer > Options Fig. 7-10: Sample transfer > Options Aspirate from bottom Elution from filter Dispense from top Change Tips when command finished before asp. for next destination well before each aspiration Dip tips Aspirate liquid from the base of the vessel. When using several vessels with different volumes, enter the lowest filling volume of these vessels (Sample transfer > Parameter). Aspirate liquids from the supernatant in filter plates. Available if in the Parameter window the Pipette radio button is selected. During liquid dispensing, position the pipette tips 3 mm 4 mm below the edge of the vessel. When using several vessels with different volumes, enter the highest filling volume of these vessels (Sample transfer > Parameter). Defined time when the pipette tips are changed. Eject pipette tips after the end of the command. The liquid will be aspirated with the same tip from all source labware positions. Eject the pipette tips before the liquid from the next source position is aspirated. Liquid from the same source position will be aspirated with the same tip. Liquid from the next source position will be aspirated using a new tip. Eject pipette tips prior to each aspiration. Liquid will always be aspirated with a new tip. Additional volume will be discarded in the waste container along with the pipette tip. After liquid dispensing, a drop may remain on the pipette tip. The drop is stripped off at the surface of the liquid. Adjust, how close the pipette tip may approach the surface of the liquid after liquid dispensing. The surface of the liquid has the value zero. Positive values indicate the distance from the surface of the liquid into the liquid. Negative values indicate the distance from the surface of the liquid to the top.

50 50 epblue Application Editor - Creating and editing applications keep tips, do not change tips after XX aspirations Restore tips Reuse tips Do not eject pipette tips. Liquid will be aspirated with the same tip from all source positions. The tips are also used in the next command. If the transfer type changes in one of the following commands, or the Mix command is used, the tips will be ejected. Pipette tips will be ejected after a defined number of aspirations. Pipette tips are restored to the position of the box they were taken from. Is only executed if the tips within an application are reused for the same source vessel. The restore tips checkbox is only available with specific settings of the Change Tip option. Restored pipette tips will be reused. The pipette tips will be reused within an application for the same source vessel. Is only executed if the restore tips option was selected in a previous command and the restored tips are available. The re-use tips checkbox is only available with specific settings of the Change Tip option. Information on the aspiration and transfer of liquids can be found in the hardware operating manual.

51 epblue Application Editor - Creating and editing applications Sample transfer > Mix command The Mix command is also available for mixing (see p. 66). Abb. 7-11: Sample transfer > Mix Fig. 7-11: Sample transfer > Mix Mix before aspirating Mix after dispensing No. of Cycles Speed Volume Fixed height Aspiration Dispense Mix before each aspiration. Set the parameters following activation. If you transfer a larger volume than the maximum volume of the pipette, the liquid will be mixed before the first aspiration. Mix after each dispensing. Set the parameters following activation. Available for Transfer type > Pipette. If you transfer a larger volume than the maximum volume of the pipette, the liquid will be mixed after the last aspiration. Number of mixing cycles. Mixing frequency. If no entries were made for Speed, the defined aspiration speed of the liquid type will be used. Mixing volume. Position of the pipette tip on the Z-axis. Fixed aspiration level and dispensing level. Only for levels below the working volume. Distance between the pipette tip and base of the vessel during aspiration. If 0 mm is entered, the distance will be corrected to 2 mm. The correction is dependent on the bottom tolerance and type of labware. Distance between the pipette tip and base of the vessel during dispensing. If the dispensing position is above the vessel, the distance will be corrected to the height of the vessel. Information on mixing liquids can be found in the hardware operating manual.

52 52 epblue Application Editor - Creating and editing applications Sample transfer > Rinse options Abb. 7-12: Sample transfer > Rinse options Fig. 7-12: Sample transfer > Rinse options You can clean used pipette tips using the Sample transfer > Rinse options command. The pipette tips are rinsed in washing solution after the liquid transfer. If the re-use tips checkbox is activated in the Sample transfer > Options window, the pipette tips will be placed on the rack they were taken from after being cleaned. If the checkbox is not activated, the pipette tips are disposed of after cleaning. You can set the parameters for this with the Sample transfer > Options command. Rinse tips before disposal checkbox No. of Cycles Speed Volume Liquid type Table Rinse racks Clean pipette tips. Number of cleaning cycles. Cleaning speed. If no Speed value is entered, epblue uses the aspiration speed of the liquid type. Volume of the washing solution that is aspirated into the pipette tip. Liquid type of the washing solution. Vessel for washing solution.

53 epblue Application Editor - Creating and editing applications Sample transfer > Liquid types Liquid types are defined in epblue. Information on dispensing liquids can be found in the hardware operating manual. Tab. 7-1: Defined liquid types in epblue Liquid type: Optimized for 50 µl tips: 50 µl tips: Comments Pipetting from Dispensing from Alcohol 75% Mixture of 75 % ethanol and 25 % water 1 to 3 μl 3 μl Wash reagent in kits for nucleic acid purification. See applications. Alcohol 98% Alcohol 98 % 1 μl 3 μl New pipette tips are pre-wetted with the liquid to be aspirated. Prewetting option is set (see p. 55). If you are using pipette tips with 300 μl in Multidispense mode and aspirate a volume of 300 μl, the gap to the filter is very small. The filter may be wetted. For 300 μl pipette tips and an aspiration volume > 280 μl, use the Pipette dispensing mode. Glycerol Protein Mixture of 40 % glycerol and 60 % water Water with 1 % albumin (10 g/l), 0.01 % Triton X μl 5 μl Liquid type for enzyme solutions. If the glycerol content of the enzyme solutions is significantly lower, liquid type Water can be used. 5 μl 5 μl New pipette tips are pre-wetted with the liquid to be aspirated. Prewetting option is set (see p. 55). The bending of the surface of the liquid has a negative impact on the free jet capability of dispensing in cell culture plates.

54 54 epblue Application Editor - Creating and editing applications Liquid type: Optimized for 50 µl tips: 50 µl tips: Comments Pipetting from Dispensing from ProteinC Rinse Speed_xl Speed_xs Water Water with 1 % albumin (10 g/l), 0.01 % Triton X-100 Demineralized water. Water with low surfactant content. Demineralized water. Demineralized water. Demineralized water. 5 μl 5 μl New pipette tips are pre-wetted with the liquid to be aspirated. Prewetting option is set (see p. 55). Dispensing the liquid with a greater distance to the calculated plane surface of the liquid (4 to 5 mm) than liquid type Protein. All other data such as liquid type Protein Recommended for nutrient media. 1 μl 3 μl Low aspiration and dispensing speed, strongly delayed blow-out. Other data such as liquid type Water. This liquid type is suitable if the mixing parameters of a transfer command or the Mix command are used. Can increase the risk of contamination for small vessels, e.g., PCR plates. Recommended in combination with the Mix option for reducing the residual liquid in the pipette tip. 1 μl 3 μl Mixing at high dispensing speed. High dispensing speed poses a high risk of contamination for small vessels. Good mixing in DWP 96 with 750 μl aspiration and 750 μl dispensing. 1 μl 3 μl For slow aspiration from filter plates. Very low aspiration speed. Sediment will not be dispersed. 1 μl 3 μl Recommended for most applications. Technical data on the systematic and random errors were determined using this liquid type.

55 epblue Application Editor - Creating and editing applications Changing the liquid type Changes to the liquid type have effects on the accuracy of pipetting. Changing the settings may increase the dispensing deviations. Abb. 7-13: Sample transfer > Liquid types Fig. 7-13: Sample transfer > Liquid types To change the liquid type, check the Change parameter checkbox. Enter the aspiration speed of the liquid in the Aspiration speed field: 0.2 mm/s 110 mm/s range. For viscous solutions and large aspiration volumes, select a low aspiration speed. The liquid aspiration is delayed. The liquid aspiration must be ended before the pipette tip withdraws from the liquid. Select the low aspiration speed during phase separations or to prevent the distribution of sediments or particles. Enter the dispensing speed of the liquid in the Dispense speed field. Input range: 0.2 mm/s 110 mm/s Select a low dispensing speed if you dispense larger volumes in empty vessels. A lower dispensing speed decreases the risk of liquid spraying back. Use larger vessels to achieve better mixing results due to a higher speed. Enter the blow-out delay in the Blow delay field. Input range: 0 ms ms. The delay is the break between the end of the dispensing during liquid dispensing and blow-out. This setting has an effect on the Transfer type Pipette. If you use liquids with high wetting behavior (e.g., solutions which contain proteins or detergents, organic solvents) and delayed drain-off characteristics (highly viscous liquids), delay the blow-out. The residual liquid collects in the lower part of the tip prior to the blow-out. If you use liquids with low wetting behavior (e.g., water, saline solutions), set the blow-out delay to 0. Enter the dispensing speed of the blow-out in the Blow speed field. Input range: 0.2 mm/s 110 mm/s. Using liquids that tend to form bubbles on the outlet of the pipette tip increases the dispensing speed of the blow-out. Decrease the dispensing speed if liquid sprays back into the vessel. This setting has an effect on the Transfer type Pipette. In the Blow movement field, enter the path of the piston during blow-out. Input range: 0 % 100 % as percentaged value of the setting Initial stroke. No blow-out will be carried out during when 0 is entered. The entire path of the piston is part of the dispensing step.

56 56 epblue Application Editor - Creating and editing applications When entering 100, the entire piston path is available for the blow-out. The blow-out is conducted using the Blow delay and Blow speed parameters. Increase and decrease the Blow movement along with the Blow speed setting. This setting only has an effect on the Transfer type Pipette. In the Initial stroke field, enter the path the piston takes before the liquid aspiration. Input range: 0 % 100 % of the available path, depending on the type of dispensing tool. The higher the value, the larger the amount of piston path that is available for the blow-out after liquid dispensing. To decrease the amount of residual liquid in the pipette tip after dispensing, increase the value. To decrease the formation of foam during dispensing, decrease the value. Reducing the value can increase the dispensing deviation. The entry 0 means that no blow out will be conducted. If you change the value, the pipette tips will be replaced before liquid aspiration. In the Prewetting field, enter the number of piston movements for pre-wetting the pipette tips. Input range: 0 9 cycles. Non-wetted pipette tips can be pre-wetted to create the same conditions for dispensing. Wetting the pipette tips: For liquids with low vapor pressure. For liquids with reduced surface tension and delayed drain-off characteristics.

57 epblue Application Editor - Creating and editing applications Testing amended liquid types on the epmotion Prerequisites The parameters of the liquid types are customized to your requirements. 1. Carry out the sample run of the application with demineralized water. 2. Check the precision and accuracy of the dispensing. 3. Check the working volume of the labware. No liquid may spray out of the labware. 4. Carry out the sample run of the application using the provided liquid. 5. Check the precision and accuracy of the dispensing. 6. Check the working volume of the labware. No liquid may spray out of the labware Reagent transfer command The Reagent transfer command is used to transfer a liquid from one or more source positions into several destination positions. After the first source position has been emptied, the epmotion switches to the next source position. Abb. 7-14: Principle of the Reagent transfer command Fig. 7-14: Principle of the Reagent transfer command When using multiple source positions and the optical sensor is switched on, the volume of the first source position will be checked when starting the application run. If there is not enough liquid available for the number of samples, the Checkrun window opens. To incorporate the next source position in the calculation, select accept level and continue. The optical sensor checks the next source position. The calculated volumes will be summed. The application will start if the volume is sufficient. The Number of samples command is used to define the number of destination vessels. The parameters of the command Reagent transfer are identical to the command Sample transfer (see p. 47). If the Pierce settings function is activated in the properties of the labware, the labware will be opened before the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened, the source vessel is opened first. To open the vessels, epblue uses the same dispensing tool and the same pipette tips as for liquid transfer. Proceed as follows to define the pattern for the Reagent transfer command: 1. Select the source position in the source labware. If you are using several source positions, select these vessels one after the other. 2. Select the destination position in the destination labware. Source position and destination position for one pattern are the same color.

58 58 epblue Application Editor - Creating and editing applications Dilute command The Dilute command is used to create serial dilution series, e.g., for PCR standards. A defined volume is pipetted several times from one vessel to the next. Abb. 7-15: Principle of the Dilute command Diluent Diluent Diluent Undiluted Sample 1. Dilution 2. Dilution 3. Dilution Fig. 7-15: Principle of the Dilute command The parameters of the command Dilute are identical to the command Sample transfer (see p. 47). If the Dilute command is carried out within one labware the source positions and destination positions must not overlap. If the Pierce settings function is activated in the properties of the labware, the labware will be opened before the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened, the source vessel is opened first. To open the vessels, epblue uses the same dispensing tool and the same pipette tips as for liquid transfer. 1. Fill the destination positions with a diluent. Use the Reagent transfer command. 2. Define the number of samples to be diluted Using the Number of samples command. 3. In the pattern, define the number of dilution steps. The pattern is limited by a series or column. 4. Enter the options for the Dilute command. 5. To achieve a good mixture of sample and diluent, activate the Mix > Mix after dispensing option. The sample and diluent will be mixed after each dispensing. 6. To mix the undiluted sample before the first aspiration, activate the Mix before aspirating option. Proceed as follows to enter the pattern for the Dilute command: 1. Select the source position of the undiluted sample. 2. Select the destination positions of the dilution steps. The number of positions defines the number of dilution steps. 3. Repeat the procedure for each source position.

59 epblue Application Editor - Creating and editing applications Example of a dilution series This example explains the concept of a dilution series. The example does not describe the full application. Objective 24 samples are in a rack with 24 positions and should be diluted 1:1000. The dilution should be completed in 3 program steps with dilutions of 1:10. First, a single-channel dispensing tool is used. An eight-channel dispensing tool will then be used to reach a higher speed. Procedure 200 μl of each of the samples will be transferred to the 96-well plate using the Sample transfer command. The dilutions are carried out in this plate. 225 μl of the diluent will be transferred from the 300 ml reservoir to each of the empty wells of the 96-well plate using the Reagent transfer command. 25μL of the sample will be aspirated from column 1 using the Dilute command and will be mixed with respectively 225 μl of the diluent in the columns 2, 3 and 4. The mixing process will be repeated twice in the following columns. In this example, each dilution step is a 1:10 dilution. The required 1:1000 dilution will be achieved with the third 1:10 dilution. The volume aspirated from the undiluted sample also applies for the dilution steps. Creating dilution series Transferring undiluted samples to the destination labware 1. Pattern of the Sample transfer command: The source position and destination position are the same color. epblue detects the pattern and suggests the next field.

60 60 epblue Application Editor - Creating and editing applications Transferring diluent to the destination labware 2. Pattern of the Reagent transfer command: With an eight-channel dispensing tool, the Reagent transfer command is used to aspirate the diluent from the 300 ml reservoir and dispense it into the empty wells of the 96-well plate. Carrying out the dilution 3. Pattern of the Dilute command: Using an eight-channel dispensing tool, liquid is removed from column 1 and mixed with the diluent in columns 2, 3 and 4. This procedure will be repeated in the following columns.

61 epblue Application Editor - Creating and editing applications Pool command The Pool command can be used to combine liquids from several source positions in one or more destination positions. Abb. 7-16: Principle of the Pool command Fig. 7-16: Principle of the Pool command The parameters of the command Pool are identical to the command Sample transfer (see p. 47). If you are using the Multiaspirate function, liquid will be removed from the source positions until the pipette tip is filled. The contents will then be dispensed in the destination position. In the pattern, you define which source positions will be pooled in the destination position. The Number of samples command before the Pool command defines the number of source positions. Options > Change Tips is set to before asp. for next destination well. If the Pierce settings function is activated in the properties of the labware, the labware will be opened before the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened, the source vessel is opened first. To open the vessels, epblue uses the same dispensing tool and the same pipette tips as for liquid transfer Pool one destination command The Pool one destination command can be used to combine liquids from several source positions in one destination position. This command is a simplified Pool command. The parameters of the command Pool one destination are identical to the command Sample transfer (see p. 47). The Number of samples command before the Pool one destination command defines the number of source positions. The Options > Change Tips option is set to when command finished. The pipette tips are ejected at the end of the command. If the Pierce settings function is activated in the properties of the labware, the labware will be opened before the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened, the source vessel is opened first. To open the vessels, epblue uses the same dispensing tool and the same pipette tips as for liquid transfer.

62 62 epblue Application Editor - Creating and editing applications Pierce command You can open the sealing of individual positions of a labware. The labware must be sealed with suitable aluminum foil. The epmotion opens the sealing with a single-channel dispensing tool. You can open the sealing of individual positions using the Pierce command. A liquid transfer does not have to be performed directly after the Pierce command. epblue saves, on which positions of the labware the sealing was opened. This way, the sealing of each position of the labware can only be opened once. You have to activate the Pierce settings function in the properties of the labware to open the sealing with the Pierce command. You have to adapt the parameters of the Pierce settings function to the labware combination and the aluminum foil. Abb. 7-17: Pierce > Options command Fig. 7-17: Pierce > Options command Pipet. tool checkbox Select dispensing tool Pattern button Select Pattern mode to open the labware Racks checkbox Select Labware

63 epblue Application Editor - Creating and editing applications 63 Abb. 7-18: Pierce > Parameter command Fig. 7-18: Pierce > Parameter command Change tips radio button when command finished Replace pipette tips if all positions have been opened. after each perforation Replace pipette tips if a position has been opened. keep tips Do not replace pipette tips. restore tips checkbox Pipette tips are restored to the position of the box they were taken from. Is only executed if the tips within an application are reused for the same source vessel. The restore tips checkbox is only available with specific settings of the Change Tip option. re-use tips checkbox Restored pipette tips will be reused. The pipette tips will be reused within an application for the same source vessel. Is only executed if the restore tips option was selected in a previous command and the restored tips are available. The re-use tips checkbox is only available with specific settings of the Change Tip option.

64 64 epblue Application Editor - Creating and editing applications Comment command Abb. 7-19: Comment command Fig. 7-19: Comment command Comment field Enter comment A comment can be output using the Comment command. The comment will be shown when the command is executed Exchange command The command Exchange is only available for epmotion Abb. 7-20: Exchange command Fig. 7-20: Exchange command Labware drop-down menu Labware taken out of the epmotion Exchange with drop-down menu Labware to be placed inside the epmotion The Exchange command can be used to exchange labware. When the command is executed, epblue prompts you to replace the labware manually.

65 epblue Application Editor - Creating and editing applications Magnetic separation command The Magnetic separation command only is available for devices with magnetic finger module. The Magnetic separation command can be used to separate magnetic beads using the magnetic finger module. The Magnetic separation command can only be executed if the PrepRack is positioned on the Eppendorf ThermoMixer module of the epblue worktable. The efficiency of the magnetic separation depends on the properties of the used beads. The Duration should be at least 2 min. If the Magnetic separation command is executed when the Separation On checkbox is checked, the magnetic fingers will be drawn out. During the waiting period, the magnetic beads will be separated by the drawn out magnetic fingers on the tube inner wall. After the waiting period has elapsed, the next command in the procedure will be executed. If the Magnetic separation command is executed when the Separation On checkbox is not checked, the magnetic fingers will be drawn in. Abb. 7-21: Magnetic separation command Fig. 7-21: Magnetic separation command Separation enabled checkbox Check the checkbox to start the separation Uncheck the checkbox to end the separation Duration field Waiting period. The application stops until the waiting period has elapsed. Starting the separation To start the separation, execute the Magnetic separation command when the Separation On checkbox is activated. The magnetic fingers will be drawn out. Exiting the separation To end the separation, execute the Magnetic separation command when the Separation On checkbox is deactivated. The magnetic fingers are drawn in.

66 66 epblue Application Editor - Creating and editing applications Mix command The Mix command can be used to mix liquids in a position by multiple aspiration and dispensing steps. The options of the Mix command are identical with the options of the Sample transfer command in the Mix area (see p. 51) Number of samples command The Number of samples command is used to define how many samples or positions will be processed in the following transfer commands. The Number of samples command is valid until it has been replaced by a new Number of samples command. You can use several Number of samples commands within one application. When the application starts, the software will query entries for the commands Number of samples. If you do not want to execute part of the application, enter 0. If you do not use the Number of samples command, epblue asks you for the number of samples at the start of the application. This entry then applies for all commands of the application. The number of samples is limited by the pattern. Abb. 7-22: Number of Samples command Fig. 7-22: Number of Samples command Fix Sample Count checkbox Check the checkbox in order to define the number of samples Uncheck the checkbox to enter the number of samples at the start of the application. Number of Samples field Active if the Fix number of samples checkbox is checked. Enter the number of samples. This value will be taken into account for the pattern. Max Number of Samples field Active if the Fix number of samples checkbox is not checked. Maximum number of samples that can be processed in the application. Comment field This text field is displayed when the software asks for the number of samples. Enter explanatory information in the field.

67 epblue Application Editor - Creating and editing applications 67 In accordance with the subsequent command, the Number of samples command has the following effects: Prior to the Sample transfer command: Number of samples aspirated from the source labware. Prior to the Reagent transfer command: Number of destination positions liquid is dispensed into. Prior to the Pool or Pool one destination command: Number of source positions from which liquid is removed. Prior to the Dilute command: Number of samples to be diluted. The number of dilution steps is defined by the pattern. Prior to the Mix command: Number of source positions in which liquid is mixed. With an eight-channel dispensing tool: 8 samples are processed for values 1 8. For values 9-16, 16 samples are processed, etc Temperature command The command Temperature only is available for devices with thermal module. With the Temperature command, you can temper the labware in a thermal module. The temperature control stops when epblue is exited. Abb. 7-23: Temperature window Fig. 7-23: Temperature window Location drop-down list Select a thermal module. Temperature on checkbox Activate the thermal module. Activated thermal modules are switched off when you uncheck the checkbox. Keep Temperature after application run checkbox Activate the thermal module for the period after the application has exited. This function can be used to cool samples after the application run has been completed. Temperature field Set the set temperature of the thermal module. 0 C 110 C range.

68 68 epblue Application Editor - Creating and editing applications ThermoMixer command The ThermoMixer command is only available for devices with Eppendorf ThermoMixer module. The ThermoMixer command can be used to set the temperature and speed of the Eppendorf ThermoMixer module. Abb. 7-24: ThermoMixer window Fig. 7-24: ThermoMixer window Template drop-down list The labware library contains templates for the Eppendorf ThermoMixer module. Mixing on checkbox Activate the mixing process of the Eppendorf ThermoMixer module. Speed field Set the mixing frequency. 300 rpm 2000 rpm range. Duration field Set the mixing time. 5 s 120 min range. Temperature on checkbox Activate the tempering of the Eppendorf ThermoMixer module. Keep temperature after applicaton run field Activate the temperature control for the period after the end of the application. This function can be used to cool samples or reagents after the end of the application. Temperature field Set the set temperature of the Eppendorf ThermoMixer module, 4 C 95 C range. Information on the Eppendorf ThermoMixer module can be found in the epmotion hardware operating manual.

69 epblue Application Editor - Creating and editing applications 69 NOTICE! Contamination of samples and the device due to excessive speeds. If the speed is set too high, liquid sprays out of the vessels. Labware may become loose from the holder and fly around. Observe the labware maximum speeds. Tab. 7-2: Maximum speed for labware on the Eppendorf ThermoMixer module Labware Maximum speed in rpm Vessels 1000 Deepwell plates with 96 wells 1200 Deepwell plates with 384 wells 1200 Microplate with 6 wells 1000 Microplates with 96 wells 2000 Microplates with 384 wells 2000 PCR plate with 96 wells 2000 PCR plate with 384 wells 2000 ReservoirRack Mixing not possible Thermorack or Thermoblock 1000 Thermoblock 96 with a semi-skirted PCR plate for 250 μl 1700 Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/V 1600 Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/U 1600 Thermoblock PCR 96 OC 1700 Thermoadapter for 96 wells 1000 Thermoadapter for 384 wells 1000 Thermorack TMX 1300 PrepRack Creating a template for the Eppendorf ThermoMixer module Prerequisites The ThermoMixer command is selected (see p. 68). 1. Edit the template in the Options field. The word Changed appears next to the Save button. 2. Enter the name of the template. 3. Click on the Save button. The template is saved.

70 70 epblue Application Editor - Creating and editing applications Managing templates for the Eppendorf ThermoMixer module Abb. 7-25: Labware Editor > TMX Templates Fig. 7-25: Labware Editor > TMX Templates Manage the templates for the Eppendorf ThermoMixer module in the Labware Editor > TMX Templates folder Transport command The command Transport only is available for devices with gripper. The Transport command can be used to transport labware. The gripper takes labware from one location and transports it to another location. Abb. 7-26: Transport window Fig. 7-26: Transport window Labware Select the labware. To location Select a location. Information on the labware that is being transported can be found in the hardware operating manual.

71 epblue Application Editor - Creating and editing applications User intervention command The User intervention command is used to interrupt an application. The application will be continued when the user confirms a message. You can use the User intervention command to empty the waste container manually. Do not change the filling volume during the interruption. If you need to change the filling volume, use several applications to do so. Do not change the equipping of the worktables. Abb. 7-27: User Invention window Fig. 7-27: User Invention window Comment window The comment appears when the command is executed. Alarm checkbox An acoustic signal sounds if the command is executed.

72 72 epblue Application Editor - Creating and editing applications External process command With the External process command, labware can be removed from the device. The labware can be further processed outside of the epmotion. The application waits until the labware is reset. Abb. 7-28: External process command Fig. 7-28: External process command Labware drop-down list Select labware to be removed. Comment field Enter comments Rescan location checkbox The optical sensor checks if the reset labware is positioned correctly.

73 epblue Application Editor - Creating and editing applications Wait command The Wait command is used to create a waiting period. The application will be continued after the waiting period. The command can be used to take into account incubation periods between the addition of reagents. Abb. 7-29: Wait window Fig. 7-29: Wait window Duration field Waiting period. Wait for temperature checkbox The waiting period begins after the thermal module has reached the set temperature. Location drop-down list Thermal module whose temperature control is waited for. 7.4 Pattern For the pattern, enter the source position and destination position between which liquid transfers take place. Type of pattern Regular pattern Irregular pattern Description Available for all commands. With regular pattern. epblue detects and completes the pattern. Not available for ReservoirRacks and ReservoirRack modules. Available for some commands. Pattern according to a random pattern. epblue cannot detect and complete the pattern. Replicates not possible. Whether you can define regular or irregular patterns depends on the command and the labware.

74 74 epblue Application Editor - Creating and editing applications Tab. 7-3: Tab. 7-4: Pattern options with regular labware Command Source labware pattern Destination labware pattern Sample transfer Regular and irregular Regular and irregular Reagent transfer Regular and irregular Regular and irregular Pool Regular Regular and irregular Pool one destination Regular and irregular Regular and irregular Dilute Regular and irregular Regular Mix Regular and irregular Regular and irregular Pattern options with a ReservoirRack Equipped with ReservoirRack module for Pattern ReservoirRack Reservoir 30 ml Regular Reservoir 100 ml ReservoirRack Individual vessels Irregular Menu bar in the Edit Pattern window Abb. 7-30: Menu bar in the Edit Pattern window Fig. 7-30: Menu bar in the Edit Pattern window Reset Reject pattern. Complete Regular patterns are completed automatically. The pattern is ended when the number of samples defined in the Number of Samples command is reached. Next arrow One step forward. Back arrow One step back. Preview Preview of the pattern with an increased number of samples. Show Show the pipetting sequence Defining regular patterns Prerequisites Labware is positioned on the worktable. Transfer command or Mix command activated. 1. Select the source labware in the Source area. 2. Select the destination labware in the Destination area. 3. Click the Pattern button. The Edit Pattern window appears. 4. To delete an existing pattern, press the Reset button. If you work with an eight-channel dispensing tool, select the top position. For a 96-well pate, this is row A. For a 384-well plate, this is rows A or B. epblue adds the pattern for the remaining channels.

75 epblue Application Editor - Creating and editing applications Select the first source position in the source labware. 6. Select the first destination position in the destination labware. To create replicates, select several destination positions one after the other. Abb. 7-31: Selecting the first source position Abb. 7-32: Selecting the first destination position Fig. 7-31: Selecting the first source position Fig. 7-32: Selecting the first destination position 7. Select the next source position in the source labware. 8. Select the next destination position in the destination labware. Abb. 7-33: Selecting the next source position Abb. 7-34: Selecting the next destination position Fig. 7-33: Selecting the next source position Fig. 7-34: Selecting the next destination position epblue attempts to detect the pattern and marks the next position gray. If you created replicates during the first step, and select the destination position for the second liquid transfer, the positions are defined for the replicates in the same order as in the first step. 9. If the pattern matches your requirements, press the Complete button. The pattern is completed automatically up to the defined number of samples. If you would like to discard the pattern, press the Reset button.

76 76 epblue Application Editor - Creating and editing applications 10.To check a pattern, press the Show button. The order of the pattern is displayed. The source position and destination position of a liquid transfer are the same color Defining irregular patterns Irregular patterns can be defined for source labware and destination labware. If the pattern is irregular, it is not completed. Prerequisites Labware is positioned on the worktable. Create the application with the Transfer or Mix command (see p. 47). 1. Click the Pattern button. Abb. 7-35: Edit Pattern window Fig. 7-35: Edit Pattern window The Edit Pattern window appears. 2. Check the Source checkbox in the Irregular pattern area. 3. Optional in the Destination area: check the Irregular pattern checkbox. 4. Define the pattern. Specify all source positions and destination positions. If the pattern is irregular, the software does not complete the pattern automatically. The Complete button is not active.

77 epblue Application Editor - Creating and editing applications Optical sensor The principle of operation of the optical sensor is described in the hardware manual. The optical sensor has the following functions: Measure fill level in vessels The optical sensor determines the filling level of the labware positions. There are 2 ways of doing this: The optical sensor determines the filling level in all positions used. The optical sensor determines the filling level in the first position, in the last position and in 8 randomly determined positions. The optical sensor cannot measure the fill level in the following labware: 384-well plates, Universal Disc from Focus Diagnostics, Qiagen rotor disc 72, 100. Detect pipette tips The optical sensor determines the quantity and the positions of the ep.t.i.p.s. pipette tips. Motion pipette tips in TipHolders and Racks by Eppendorf. Check labware on the worktable The optical sensor scans the code on the labware. epblue checks if the labware on the epmotion worktable matches the labware on the epblue worktable. If the optical sensor is switched off, the processing time of the application is reduced Measuring the filling level of the labware The Detect volumes function enables you to measure the filling volume in the labware positions The optical sensor detects the filling volume up to the detection limit. The detection limit depends on the labware geometry. Generally, levels of 3 mm or higher can be detected. Each labware definition contains information on the detection limit of the filling volume. If the filling volume in one position is below the detection limit, you have to enter the filling volume manually. The optical sensor can execute the Detect volumes function based on different parameters. You define these parameters as application parameters when you create an application. When you start a run, you can change the application parameters. You can define individual run parameters for each run. Application parameters Run parameter Defined in the Application Editor. Defined in the Application Runner. Defined when creating the application (see p. 78) Defined when starting the run (see p. 80). Defined individually for each labware used in the Applies for the complete labware of the application. application. Different parameters can be defined for each run.

78 78 epblue Application Editor - Creating and editing applications Defining application parameters for measuring the filling volume of plates Abb. 7-36: Properties window for plates Fig. 7-36: Properties window for plates Tab. 7-5: Off All positions Random positions Parameters of the Detect volumes function Deactivate the optical sensor. The filling volumes of all positions must be entered when the application is started. For labware with 56 positions, the filling volume for each position can be specified in the Volume column. For labware with > 56 positions, one filling volume can be specified for all positions. This filling volume is recommended at the start of the application, and can be changed. The volume specification does not take into account the surface meniscus of the liquid. If the filling volume is not enough, the result may be faulty dispensing. Checks the filling volume in all positions used in the application. If you work with a single-channel dispensing tool, epblue uses the calculated filling volume for each position. If you work with a multi-channel dispensing tool, the epblue uses the lowest calculated filling volume in a column for liquid aspiration and the highest calculated volume in a column for liquid dispensing. The optical sensor checks the filling volume of the first, last and 8 randomly selected positions. For the parameter to be available, the labware has to have at least 24 positions. If the filling volumes in one plate differ significantly or the labware has very low filling volumes, use the Aspirate from bottom and Dispense from top options. To set the application parameters for the Detect volumes function (see To change the properties of a labware, double-click on the labware. on p. 40).

79 epblue Application Editor - Creating and editing applications Application parameters for measuring filling volume of special labware Abb. 7-37: Properties window for special labware Fig. 7-37: Properties window for special labware The optical sensor cannot measure the filling level in the following labware: 384-well plates Universal Disc by Focus Diagnostics Qiagen Rotor-Disc 72 and Qiagen Rotor-Disc 100

80 80 epblue Application Editor - Creating and editing applications Defining the run parameters for measuring the filling volume Abb. 7-38: Run parameters when starting the application Fig. 7-38: Run parameters when starting the application Detect volumes radio button The optical sensor measures the filling volume. In which positions the optical sensor measures the filling volume is defined in the labware properties. The settings made in the labware properties are overwritten with the run parameters. Use required minimum volume radio button epblue assumes that all vessels are filled with the minimum required volume for this run. Input volumes manually radio button Enter the filling volume manually. Detect tips slider Check-in which positions pipette tips are located in the labware. Check labware placement slider Check the labware on the worktable To set the run parameters for the Detect volumes function (see Setting the run parameters for the optical sensor on p. 26). Examples: Prerequisites Application parameters Off Run parameter Detect levels The filling volume is measured at the start of the application. Prerequisites Application parameters All positions Run parameter Input level manually The filling volume must be entered manually when the application is started.

81 epblue Application Editor - Creating and editing applications Detecting pipette tips You can only use the Detect tips function, if you are using ept.i.p.s. Motion in TipHolders and racks by Eppendorf. Enter the Detect tips function as a run parameter at the start of the application (see Setting the run parameters for the optical sensor on p. 26). The Detect tips function checks the type of pipette tips and determines the number and position of tips in the rack. The ept.i.p.s. Motion tray is coded. The optical sensor uses the code to detect the type of pipette tips. Tab. 7-6: Arrangement of pipette tips if the Detect tips function is activated A A A B B B C C C D D D E E E F F F G G G The Detect tips function checks to see if there are enough pipette tips available for the application. The pipette tips must be continuously positioned without gaps. epblue prompts you to refill the pipette tips after the available tips have been depleted. In the next application, you can continue using a rack that has already been opened. Tab. 7-7: Arrangement of pipette tips if the Detect tips function is deactivated A A A B B B C C C D D D E E E F F F G G G If the Detect tips function is deactivated, the columns of the rack must be fully equipped. The tips must be available as of position A Checking the labware on the worktable Enter the Check labware placement function as a run parameter when starting the application (see Setting the run parameters for the optical sensor on p. 26). The Check labware placement function detects the labware coding. The function checks if the labware on the epmotion worktable matches the equipment of the epblue worktable.

82 82 epblue Application Editor - Creating and editing applications

83 epblue Application Editor - Managing applications and folders 83 8 epblue Application Editor - Managing applications and folders This chapter describes how to manage applications in epblue. Folders are managed according to the same rules. 8.1 Menu bar and start screen Abb. 8-1: Application Editor menu bar Fig. 8-1: Application Editor menu bar Creating a new application Cutting an application or folder Creating a new folder Pasting an application or folder Opening an application Deleting an application or folder Editing the application properties Importing an application Copying an application or folder Exporting an application Abb. 8-2: Start screen in the Application Editor Fig. 8-2: Start screen in the Application Editor

84 84 epblue Application Editor - Managing applications and folders 8.2 Copying and deleting applications Copying applications You can copy applications. The Eppendorf folder contains applications defined by Eppendorf AG. These applications are used as templates and program examples. Copy this application into your area in order to modify and execute it. Prerequisites The start screen in the Application Editor is open. 1. Select the desired application. 2. Click on the symbol in the menu bar. 3. Select the folder in which the application is. 4. Click on the symbol in the menu bar. The application appears in the selected folder Deleting an application You can delete an application. Prerequisites The start screen in the Application Editor is open. 1. Select the application. 2. Click on the symbol in the menu bar. 3. Confirm deleting the application. To do this, press the OK button in the message window. The application is deleted.

85 epblue Application Editor - Managing applications and folders Changing the properties and name of the application You can change the name and properties of an application. Prerequisites The start screen in the Application Editor is open. Select the application. The properties of the application appear in the Properties field. Press the symbol in the menu bar. The Edit Properties window opens. Abb. 8-3: Edit Properties window Fig. 8-3: Edit Properties window Change the application settings. To save the changes, press the Save button.

86 86 epblue Application Editor - Managing applications and folders 8.4 Importing and exporting applications Importing applications Tab. 8-1: File format *.ws or *.lhs *.dws *.export You can import applications in the following file formats: Source of file Applications from the control panel Applications from versions < 20.X of epblue Applications from versions 20.X of epblue Prerequisites A USB storage medium is connected. The start screen in the Application Editor is open. 1. Select the folder into which the application is to be imported. 2. Click on the symbol in the menu bar. The Import Applications window opens. 3. Select the applications to be imported. 4. Press the Open button. The Import Application window opens. Abb. 8-4: Import Application window Fig. 8-4: Import Application window Name area List of all applications for which import was selected. Standard application checkbox Activate if the application is to be imported. Selected checkbox Activated, if the application is an Eppendorf template. Define import rules area Define rules for the import of the applications. Materials tab Show the labware used in the applications. Import button Import applications. Cancel button Cancel the import.

87 epblue Application Editor - Managing applications and folders Define the rules for importing labware. 6. Press the Import button. The files will be imported. A summary of the import will be displayed. 7. Press the Finish button. If you import applications from older software versions, the display of the worktable in epblue may not match your device configuration. To remedy this problem, save the application under a new name using Save as Exporting applications You can export applications individually. You can export a folder with applications. Prerequisites A USB storage medium is connected. The start screen in the Application Editor is open. 1. Select the application to be exported. 2. Click on the symbol in the menu bar. The Browse For Folder window opens. 3. Select the folder in which the application is to be saved. 4. Press the OK button. The applications are exported. Exported applications can only be used with epblue from version 40.4.

88 88 epblue Application Editor - Managing applications and folders 8.5 Saving an application as a PDF file You can save an application as a PDF file. An application includes the procedure and equipping of the worktable. You can save an application protocol as a PDF file (see p. 34). Prerequisites A USB storage medium is connected. The application is open. 1. Click on the symbol. The print preview opens. The menu bar contains the following symbols: Search in the document. Show the first page. Print the document (not active). Show the previous page. Page setup. Show the next page. Scale the document. Show the last page. Zoom in the view. Create a PDF. Set the zoom factor. Close the view. Zoom out the view. 2. Check the layout and set up the page. 3. Save the document as a PDF file. 4. Close the window.

89 epblue Application Editor - Managing applications and folders Saving an application for a different epmotion model You can save an imported application to execute on your epmotion. You can create applications for a different epmotion model. You can then export these applications and import them to the software of a different model. Exported applications can only be used with epblue from version If you save applications for a specific epmotion model the epblue worktable of the application will adapt to the epmotion worktable of the selected device. Abb. 8-5: Save As window Fig. 8-5: Save As window Filename Name of the application Device model Name of the epmotion for which the application is saved Features Configuration of the epmotion for which the application is saved Prerequisites The application is open. 1. Click on the button in the toolbar. The Save as window opens. 2. Select the folder in which the application is saved. 3. Select the name and configuration of the epmotion for which the application is saved. 4. Enter the name of the application. 5. Press the Save button. The application has been saved.

90 90 epblue Application Editor - Managing applications and folders

91 epblue Labware Editor - Creating and managing labware 91 9 epblue Labware Editor - Creating and managing labware This chapter describes how to create and manage labware and labware combinations in epblue. Information on properties and use of labware can be found in your hardware operating manual. 9.1 Overview of labware The name of the labware includes information on the manufacturer and type. Example: EP_MTP_384V stands for Eppendorf_microplate_384 wells in V-Form. Tab. 9-1: Labware types Abbreviation DWP FP MTP PCR TP Cleanup DNA/RNA TT PCR Plate Thermo Quantities Description Deepwell plate Filter plate Microplate PCR plate Plate with vessels that can be removed individually. Plate from the PCR Cleanup Kit Plate from the kit for purification and insulation Eppendorf twin.tec Fixed combination of thermoblock and PCR plate Maximum filling volume of a vessel in ml or μl Tab. 9-2: Plates Labware type DWP 96 DWP 384 MTP 6 MTP 24 MTP 96 MTP 384 Labware Deepwell plate with 96 wells Deepwell plate with 384 wells Microplate with 6 wells Microplate with 24 wells Microplate with 96 wells Microplate with 384 wells

92 92 epblue Labware Editor - Creating and managing labware 9.2 Managing labware Menu bar Abb. 9-1: Menu bar in the Labware Editor Fig. 9-1: Menu bar in the Labware Editor Create new labware. Import labware. Open labware. Export labware. Delete labware. Export several labware at the same time. Display labware properties. Abb. 9-2: Start screen of the Labware Editor Fig. 9-2: Start screen of the Labware Editor

93 epblue Labware Editor - Creating and managing labware Deactivating and activating labware To keep a clear overview of your labware library in the Application Editor, you can deactivate labware that is not being used. Deactivated labware can be reactivated at any time. Applications with deactivated labware do not start. If you start an application with deactivated labware, an error message appears. Proceed as follows: 1. Open the Labware Editor. 2. Select the labware. The status of the labware is displayed in the Properties area. 3. To deactivate the labware, flip the switch from active to inactive. 4. To activate the labware, flip the switch from inactive to active Deleting labware You can delete labware that you have created. Proceed as follows: Prerequisites The Labware Editor is open. 1. In the Labware Library, select the labware to be deleted. 2. Check to see if the selected labware is activated. 3. Deactivate activated labware (see p. 93). 4. Click on the symbol to delete the labware Importing labware You can import labware definitions in zip and export formats. Proceed as follows: Prerequisites A USB storage medium is connected. The Labware Editor is open. 1. Click on the symbol in the menu bar. The Open Labware window opens. 2. Select and highlight the labware. 3. Press the Open button. The Import labware window opens.

94 94 epblue Labware Editor - Creating and managing labware Abb. 9-3: Import labware window Fig. 9-3: Import labware window Name area List of the labware selected for import. Standard labware checkbox Activated, if the labware is standard Eppendorf labware. Selected checkbox Activate if the labware is to be imported. Define import rules area Define rules for the import of the labware. Import button Import labware. Cancel button Cancel import. 4. Check selected labware definitions. Define rules for the import. 5. To import the labware definitions, click on the Import button. The labware definitions are imported to epblue. Abb. 9-4: Imported labware Fig. 9-4: Imported labware 6. End the import by clicking on the Close button.

95 epblue Labware Editor - Creating and managing labware Exporting labware You can export labware definitions. You can export individual labware definitions or the content of a folder. You can use exported labware definitions for example on another epmotion. Exported labware can only be used with epblue from version Proceed as follows: Prerequisites A USB storage medium is connected. The Labware Editor is open. 1. Select labware or folders. 2. Click on the symbol in the menu bar. Abb. 9-5: Export labware window Fig. 9-5: Export labware window The Export labware window opens. 3. Select the labware you would like to export from the Selected column. 4. Confirm the selection by pressing Save. The Browse For Folder window opens. 5. Select the folder where you want to save the labware. 6. Start export. Press the OK button. The labware is exported to the selected folder in the format.export Viewing the properties of the labware Proceed as follows: Prerequisites The Labware Editor is open. Select the labware. The properties of the labware are displayed in the Properties column.

96 96 epblue Labware Editor - Creating and managing labware Setting the bottom tolerance NOTICE! Material damage due to placing the pipette tip on the base of the vessel. If the pipette tips touch the bottom of the tube, the bottom tolerance is too low. The labware becomes damaged and the dispensing volume is distorted. Test the application without liquid. Check the bottom tolerance when exchanging pipette tips, plates, and vessels. If pipette tips become jammed at the base of the vessel, increase the bottom tolerance. If deviations in the dispensing volume occur, increase the bottom tolerance. Proceed as follows: Prerequisites The Labware Editor is open. 1. Select the labware. 2. Click on the symbol. The Edit Properties window opens. Abb. 9-6: Edit Properties window Fig. 9-6: Edit Properties window Name field Name of labware Remain Volume field Remaining volume. Is calculated. Comment field Description of labware Bottom Tolerance field Bottom tolerance. The lowest bottom tolerance is 200 μm. Eppendorf Standard Labware checkbox This checkmark is placed automatically and cannot be removed. Labware definitions from Eppendorf are write protected. Save button Save changes. 3. Change the bottom tolerance in the Bottom Tolerance field. 4. Save changes using the Save button. The settings are saved. If the labware is write protected, a copy will be saved. Copies are numbered consecutively.

97 epblue Labware Editor - Creating and managing labware Creating labware combinations Before you create an application, you have to create the required labware combination and save it in your labware library. The following options are available to you: Equipping racks, thermoracks, or ReservoirRack modules with individual vessels. Equipping ReservoirRack with reservoirs or ReservoirRack modules. Create a labware combination based on existing labware Creating a labware combination. Prerequisites The Labware Editor is open. Abb. 9-7: Create new labware window Fig. 9-7: Create new labware window Name field Name of labware Type option field Desired labware combination Comment field Description of labware 1. Click the symbol in the menu bar. The Create new labware window opens. 2. Enter the name, description, and labware combination. Racks/thermoracks + tubes Modules + tubes ReservoirRack 7 ReservoirRack 3 Equip the rack and thermorack with individual vessels. Equip the ReservoirRack module with tubes. Equip the ReservoirRack 7 with ReservoirRack modules. Equip the ReservoirRack 3 with ReservoirRack modules.

98 98 epblue Labware Editor - Creating and managing labware 3. Click the Create button. The labware combination is opened. You can create the labware combination. Abb. 9-8: Creating labware Fig. 9-8: Creating labware

99 epblue Labware Editor - Creating and managing labware Creating a labware combination based on existing labware Abb. 9-9: Start screen of the Labware Editor Fig. 9-9: Start screen of the Labware Editor Prerequisites The Labware Editor is open. 1. Select the labware. The Properties column displays information about the labware. 2. Click the symbol in the menu bar. The Edit Properties window opens. Abb. 9-10: Edit Properties window Fig. 9-10: Edit Properties window 3. Edit the properties of the labware. 4. Enter a new name in the Name field. 5. Press the Save button.

100 100 epblue Labware Editor - Creating and managing labware Equipping the racks, thermoracks, and ReservoirRack modules with individual vessels Abb. 9-11: Creating a labware combination Fig. 9-11: Creating a labware combination Racks column Racks and ReservoirRack modules. If you have selected a rack, only vessels that can be combined with the rack is shown. Tubes column Individual vessels. If you have selected an individual vessel, only racks that can be combined with an individual vessel is shown. Properties column Information on the selected labware Clear button Delete the selected individual vessel, rack, ReservoirRack module. Symbol Close the labware. Symbol Save the labware combination as new labware. Symbol Save changes. Reset button Undo changes. Return to the status before the last save. Proceed as follows: Prerequisites The labware was created with the Racks and Tubes or Module Rack + Tubes option. The labware is open. 1. Select a rack in the Racks column. 2. Select matching vessels in the Tubes column.

101 epblue Labware Editor - Creating and managing labware 101 Abb. 9-12: Rack and vessel as labware combination Fig. 9-12: Rack and vessel as labware combination 3. To save the labware combination, click the symbol in the menu bar. The labware combination is saved in the labware library.

102 102 epblue Labware Editor - Creating and managing labware Equipping the ReservoirRack with reservoirs and ReservoirRack modules Abb. 9-13: Equipping ReservoirRack Fig. 9-13: Equipping ReservoirRack Available tubs column Reservoirs and equipped ReservoirRack modules Save button Save the changed combination. Vendor Info field Properties of the selected labware. Save as button Save the new combination. Proceed as follows: Prerequisites The ReservoirRack modules were created with the Module Rack + Tubes option. The labware is open. 1. Select the reservoir or equipped ReservoirRack module from the Available tubs column. 2. Drag the reservoir or equipped ReservoirRack module to the desired position in the ReservoirRack. The epblue worktable shows which reservoirs and ReservoirRack modules the ReservoirRack has been equipped with. 3. To replace a ReservoirRack module, drag the new ReservoirRack module to the desired position. The old ReservoirRack module is automatically removed. 4. Save the labware combination.

103 epblue Labware Editor - Creating and managing labware Labware library epblue is delivered with a comprehensive labware library. The labware library contains data on the geometry of reaction vessels, pipette tips, plates, racks, reservoirs, adapters and blocks. A complete overview of the contents of the labware library from epblue can be found on the automation webpage. Eppendorf AG creates labware definitions for labware from other manufacturers. Eppendorf AG does not regularly check labware from other manufacturers. For example, if a manufacturer changes the dimensions of a vessel, the labware definition no longer matches the changed vessel Loading labware definitions from the Internet Eppendorf provides labware definitions on the webpage for download. You can use different criteria to search on the webpage. To download labware from the webpage, register in the VIP area on the webpage. To import the downloaded labware to epblue (see p. 93) Request labware definitions Eppendorf creates labware definitions for labware that you would like to use in the epmotion. You can record these definitions in the epblue labware library. 1. Check to see if the definition for your labware is in the epblue labware library. 2. Check to see if the definition for your labware is in the labware library on the automation webpage. 3. If the definition is not available for your labware, send a request to Eppendorf AG. If Eppendorf AG has a definition for your labware, you will receive the data from Eppendorf AG via . If Eppendorf AG does not have a definition of your labware, you will receive a form with questions on your labware Add a labware definition to a labware library 1. Transfer labware definitions to a USB storage medium. 2. Insert the USB storage medium in the interface. 3. Import the labware (see Importing labware on p. 93).

104 104 epblue Labware Editor - Creating and managing labware 9.5 Special labware features in epblue Location B0 on the worktable The dispensing tool cannot reach the location B0 on the worktable completely. The dispensing tool can only fill the right locations of plates and ReservoirRacks. Use the location B0 for reservoirs. Use the location B0 to stack plates Tubes Labware types in epblue: Racks with Tubes > Tubes. Reaction vessels are not available in epblue as independent labware MTP plates Labware types in epblue: Plates > MTP. Measuring the filling level is not available for MTP plates with 384 wells. For MTP plates with 96 wells, measuring the filling level takes a long time PCR plates Labware types in epblue: Plates > PCR. Labware Labware types PCR plates with 96 wells PCR 96 PCR plates with 384 wells PCR ept.i.p.s. Motion Labware types in epblue: Tips. Pipette tips with filters have a "f" added to their name in epblue. Pipette tips without filter with filter Example of names in epblue tip1000 tip1000f Racks and Safe Racks have the same labware definition.

105 epblue Labware Editor - Creating and managing labware Racks Labware types in epblue: Racks with Tubes Equip the racks with vessels before you use them in an application Rack 96 Labware types in epblue: Racks with Tubes > Racks > Rack96_1_5-2_0 The labware definition of the Rack 96 only applies to the Rack. epblue does not have a labware definition for the Rack 96 and inserted reaction vessels. Create a labware definition from the labware definition of the Rack 96 and the labware definitions of the used reaction vessels. Position the Rack 96 in row B and C, e.g. on locations B2 and C2. epblue detects that the Rack 96 occupies two locations on the worktable. Define the properties of the Rack 96 in the same way as the properties of other labware. E.g. you can define the setting of the optical sensor Rack LC Labware types in epblue: Plates > Tube Plates ReservoirRack Labware types in epblue: ReservoirRacks with Modules Only irregular patterns may be set if the ReservoirRack is equipped with different ReservoirRack modules and reservoirs. Regular patterns may be set if the ReservoirRack is equipped with identical ReservoirRack modules or identical reservoirs. For each reservoir and each ReservoirRack module, the Detect volumes function of the optical sensor can be activated.

106 106 epblue Labware Editor - Creating and managing labware ReservoirRack Module TC First, equip a ReservoirRack module with vessels. Then place the equipped ReservoirRack module in the ReservoirRack. The software only positions equipped ReservoirRack modules in a ReservoirRack. Labware types in epblue for unequipped ReservoirRack modules can be found in the folder: Racks with Tubes > ThermoRacks + Modules. Labware types in epblue for reservoirs and equipped ReservoirRack modules can be found in the folder: ReservoirRacks with Modules > Modules with Tubes. Module ReservoirRack Module TC Ø 29 mm ReservoirRack Module TC Ø 17 mm ReservoirRack Module TC Reservoir 100 ml ReservoirRack Module TC Reservoir 30 ml ReservoirRack Module TC Ø 16 mm ReservoirRack Module TC Ø 12 mm ReservoirRack Module TC Safe Lock ReservoirRack Module TC PCR 0.5 ml ReservoirRack Module TC PCR 0.2 ml ReservoirRack module TC Eppendorf Tubes 5 ml Labware type Module TC 29mm Module TC 17mm Module TC Reserv100ml Module TC Reserv30ml Module TC 16mm Module TC 12mm Module TC Safe Lock Module TC PCR 0_5ml Module TC PCR 0_2ml TC 5 ml module Thermoracks Labware types in epblue: Racks with Tubes The thermoracks must be equipped with vessels before they can be used in an application. Thermoblock/thermorack PCR 96: This labware is defined as a thermoblock and a thermorack in the labware library.

107 epblue Labware Editor - Creating and managing labware Thermorack Rotor/Tubes Order no.: Qiagen rotor disc 72: Order no.: Qiagen rotor disc 100: Order no.: The tubes in the Qiagen rotor disc are very narrow. With narrow tubes, the pipette tips can get jammed in the bottom section of the tubes. For this reason, the Qiagen rotor disc has a higher bottom tolerance. Detailed information on the bottom tolerance can be found in the labware definition. Only use the single-channel dispensing tool TS 50 when working with the Qiagen rotor disc. You can only pipette into the narrow tubes of the Qiagen rotor disc with a single-channel dispensing tool TS TipHolder Labware types in epblue: Adapter > Height. The TipHolder is an adapter that holds the ept.i.p.s. Motion Reloads. ept.i.p.s. Motion Reloads 1000 μl are positioned on the worktable using the TipHolder. To use ept.i.p.s. Motion Reloads 50 μl and ept.i.p.s. Motion Reloads 300 μl with the TipHolder, program a height adapter 40 mm on the epblue worktable. Pull the ept.i.p.s. Motion Reloads 50 μl or 300 μl onto the height adapter Thermoadapter Labware types in epblue: Adapter > Thermo In the software you can find the Thermoadapter LC Sample+Cartridge under Thermoblocks with plates. The thermoadapter and plate combination is created during the editing of the worktable or via a transfer command. With the "Thermoadapter with skirted PCR plate" combination, the plate can be transported by the gripper. The thermoadapter cannot be transported by the gripper. For the Thermoadapter Frosty, select a skirted PCR plate for the location. The cooling aggregate is not named in epblue. In the software, the Thermoadapter Frosty can be found under Adapter > Height.

108 108 epblue Labware Editor - Creating and managing labware Universal Disc by Focus Diagnostics Order no.: Universal Disc: Order number at Focus Diagnostics: MOL1401 The epmotion fills the positions on the Universal Disc with sample material. The epmotion cannot extract sample material from the positions on the Universal Disc. Work in the Pipette dispensing mode to add a second substance. If you use the Multidispense dispensing mode, the liquid transfer is not performed in an optimum way Thermoblocks Thermoblocks are heat-conductive adapters that can be transported with the gripper. There are two types of thermoblocks available. Thermoblocks with plates are thermoblocks that are permanently connected with the plate. Thermoblocks are thermoblocks, into which the plates can be inserted. Transport with the gripper Thermoblock without plates Thermoblock with plates Thermoblock with plate x x Thermoblock without plate x - Insert plate in the thermoblock x - Remove plate from the thermoblock x Thermoblock PCR 96 OC If you use the Thermoblock PCR 96 OC with a twin.tec PCR plate 96, semiskirted, you can use the TMX template PCR 96 NGS. With the TMX template you can mix with a mixing speed of 1600 rpm for 2 minutes. At a mixing speed of 1600 rpm, the maximum working volume is 140 μl. If the working volume is higher, the liquid may be spilled. The maximum mixing speed is 1700 rpm.

109 epblue settings - Checking components and making the basic settings epblue settings - Checking components and making the basic settings This chapter describes how to check components of the epmotion and make the basic settings. Prerequisites epmotion is ready for operation. epblue is started. The start screen will be displayed. 1. Press on the Settings symbol on the start screen. 2. Select the function Tool interlock The Tool interlock function can be used to check the locking mechanism of the dispensing tools and gripper. Abb. 10-1: Tool interlock function Fig. 10-1: Tool interlock function 1 Zero value sensor field 0 = Dispensing tool is not in home position. 1 = Dispensing tool is in home position. 2 Tool detection sensor field 0 = Dispensing tool not available. 1 = Dispensing tool available. 3 Interlock field OPEN = Locking mechanism opened. CLOSED = Locking mechanism closed. 4 Current Tool field Name of the tool used 5 Refresh button Update fields Lock button Lock the dispensing tool 7 Unlock button Unlock the dispensing tool

110 110 epblue settings - Checking components and making the basic settings Lock the tool 1. Slide the tool into the tool holder from below. 2. Hold the tool tightly At the same time, press the Lock button. The tool holder locks the tool Unlocking the tool 1. Hold the tool tightly At the same time, press the Unlock button. The tool holder unlocks the tool. 2. Remove the tool Dosing device The Dosing device function can be used to read out the number of stroke movements of a dispensing tool. Abb. 10-2: Dispensing Device function Fig. 10-2: Dispensing Device function Tool used Name of the tool Tool path Path of the labware definition of the tool Strokes Number of stroke movements of the dispensing tool Reading out the tool Prerequisites A tool is in the tool holder. To read out the tool data, click on the Read button.

111 epblue settings - Checking components and making the basic settings Gripper The Gripper function can be used to open and close the gripper. Labware can be removed from the gripper if necessary. Abb. 10-3: Gripper function Fig. 10-3: Gripper function Distance field Distance of gripper arms Gripper distance (µm) field Enter new distance of gripper arms Set button Confirm new distance of gripper arms Export field Exports data Moving the gripper arms Prerequisites The gripper is located in the tool holder. 1. To move the gripper arms, enter a value in the Gripper distance field. The value must be approx. 20% larger than the value in the Distance field. 2. Confirm your entry with the Set button. The gripper can now be moved.

112 112 epblue settings - Checking components and making the basic settings 10.4 UV lamp The UV lamp is used to decontaminate the epmotion worktable. An irradiation cycle lasts 15 min Decontaminating the worktable CAUTION! Risk to health due to UV radiation. UV radiation causes eye and skin damage. UV radiation may escape outward through the ventilation slits in the rear panel of the device. Wear your personal protective equipment when you are near the ventilation slits. NOTICE! Damage to plastic due to UV radiation. Plastics become brittle as a result of UV radiation exposure. Remove the dispensing tools, labware, and waste container from the worktable before switching on the UV lamp. Prerequisites The epmotion is equipped with a CleanCap. The dispensing tools, labware and waste container have been removed from the worktable. The front hood is closed. The epmotion is ready for operation. epblue has been started. 1. Select the Settings symbol on the start screen. 2. Select the UV lamp function. 3. Click on the UV Light OFF button. The UV Lamp window appears. 4. Check to see if the worktable is empty and the front hood is closed. 5. Click on the OK button to start the disinfection.

113 epblue settings - Checking components and making the basic settings 113 Abb. 10-4: Lamp symbol in status area Abb. 10-5: UV Light button Fig. 10-4: Lamp symbol in status area Fig. 10-5: UV Light button The tool holder moves. The UV lamp will be switched on. The UV Light button shows the remaining irradiation period. The lamp symbol appears in the status area HEPA air filter Air is drawn in using a fan. The air drawn in is filtered using the HEPA air filter. A continuous and filtered air flow is guided into the epmotion workspace. The air flow prevents the workspace from being contaminated with outside air Switching on the HEPA air filter WARNING! Risk of infection due to aerosols. If you work with the HEPA filter, a continuous air flow is blown from the device workspace to the environment. The air flow can transport aerosols and contaminate the ambient air. Do not use the HEPA filter when working with biohazardous substances. Disinfect contaminated surfaces in the workspace before switching on the HEPA filter. Prerequisites The epmotion is equipped with a CleanCap. All surfaces in the workspace are disinfected. The front hood is closed. The epmotion is ready for operation. epblue has been started. Select the Settings symbol on the start screen. Select the HEPA filter function. Press the HEPA filter ON button.

114 114 epblue settings - Checking components and making the basic settings Abb. 10-6: Filter symbol in the status area Fig. 10-6: Filter symbol in the status area The HEPA air filter is switched on. The filter symbol appears in the status area. The HEPA air filter remains switched on if you exit the HEPA filter function. Now start your application Switching off the HEPA air filter Prerequisites The HEPA air filter is running. The HEPA filter function on the epmotion serial number > Settings tab was selected. 1. Press the HEPA filter ON button. The HEPA air filter is switched off. 2. Alternatively, you can switch off the HEPA air filter when you switch off the epmotion using the mains/ power switch.

115 epblue Administration epblue Administration As an administrator, you can save the database, create print templates and make basic settings for the optical sensor epblue Administration Administration Starting epblue Prerequisites The epblue start screen will be shown. 1. Click on the Administration symbol. The User Login window appears. 2. Select the administrator account. 3. Enter the Admin123! password in the Password field. 4. Press the Login button. epblue Administration is started. The Administrator area appears Exit epblue Administration 1. In the File menu, select the Exit to Start Screen entry. The Administration tool will be ended. The start screen appears Changing the device name In the Devices window you will find information on the status and the configuration of the epmotion. The Devices > Settings > Detail information > Nickname shows the name of the epmotion. The serial number will be assigned as the name by default. You can change the name of the device. You can check the settings if you are logged in as an administrator. 1. Press on the Administration symbol on the start screen. 2. On the Devices tab, select the Settings function. The Detail Information window will appear. 3. Change the device name in the Nickname field. 4. To save the changes, press the Save button.

116 116 epblue Administration 11.3 Print templates Print templates can define how your print-outs and PDF documents look. Abb. 11-1: Print Header window Fig. 11-1: Print Header window Current preview setting drop-down list Select a print template. Add button Create a print template. Edit button Change a print template. Remove button Delete a print template. Show preview button Show a preview.... button Import a graphic for the header. Save button Save a print template. Cancel button Cancel the process. Save as default button Save print template as default.

117 epblue Administration Creating a print template Prerequisites You are logged in as Administrator or Admin Lab. epblue is started. 1. Click on the Administration symbol on the start screen. 2. Select the Print Header function. The Print Header window appears. 3. Click on the Edit print header template button. 4. Enter your company's address in the Company Information field. 5. Import a graphic for the header. by clicking on the button. epblue only imports graphics in the formats GIF, JPEG, and PNG. 6. To show a preview, click on the Show preview button. 7. To save the template, click on the Save button. 8. Enter the name of the template and confirm it using the OK button. The template will be saved Saving print templates as default Prerequisites You are logged in as Administrator or Admin Lab. epblue is started. 1. Click on the Administration symbol on the start screen. 2. Select the Print Header function. The Print Header window appears. 3. Select the print template in the Current preview settings menu. 4. Click on the Set as default header button. The print template is saved as standard Deleting a print template Prerequisites You are logged in as Administrator or Admin Lab. epblue is started. 1. Click on the Administration symbol on the start screen. 2. Select the Print Header function. The Print Header window appears. 3. Select the print template in the Current preview settings menu. 4. Press the Remove button. The print template is deleted.

118 118 epblue Administration 11.4 Data backup epblue saves applications, protocols, the labware library and information on user accounts in a database. The Backup function can be used to create a backup copy of the database. The backup copy can be used to restore the database after a system failure. You can save data and load a backup copy if you are logged in as administrator. NOTICE! Data loss due to missing data backup or incorrect storage of data carriers. User accounts, applications, labware and logs are stored in one database. If this database gets damaged (e.g. due to a hardware defect), the information is lost. Eppendorf is not liable for data loss of data. Eppendorf is not liable for damage arising as a result of data loss. Backup the database regularly. Only use the epblue software backup. Save stored data on external data carriers. Store the storage media according to the manufacturer's specifications Backing up the database Proceed as follows: Prerequisites A USB storage medium is connected to the EasyCon Tablet. epblue is started. You are logged in as administrator. 1. Press the Settings symbol on the start screen. 2. Select the Backup and Restore tab. 3. Select Backup. 4. Press the button. 5. Use that name for the backup or select a new name. 6. Start the backup using the Backup button.

119 epblue Administration Restoring the database You can import your backup copy in epblue. The database will be restored to the status at the time of the backup. All data in epblue is overwritten when you import the backup copy. Changes made after this backup copy was created will be lost. Abb. 11-2: Restore window Fig. 11-2: Restore window Source Full Backup Location where a backup copy is saved. Status field Shows if the software is ready or damaged. Details field Start button Imports the backup copy in epblue. Progress bar Progress of backup Prerequisites epblue is started. You are logged in as administrator. 1. Connect the USB storage medium to the control device. 2. Press the Settings symbol on the start screen. 3. Select the Backup and Restore tab. 4. Select Restore. 5. Press the button. 6. Select a backup copy with the *.bak format. 7. To import the backup copy into epblue, click on the Restore button.

120 120 epblue Administration Importing a database from epblue version 40.X You can use this function to import databases from the epblue versions 40.X into epblue from version Prerequisites epblue is started. You are logged in as administrator. 1. Press the Settings symbol on the start screen. 2. Select the Backup and Restore tab. 3. Select Migrate 40.x database. 4. Press the button. 5. Select a backup copy with the bak format. 6. To import the database into epblue, press the Migrate button Temperature control unit X The Temperature control unit X function enables you to check the thermal modules of the epmotion. Abb. 11-3: Temperature control unit function Fig. 11-3: Temperature control unit function Desired Temperature Shows the set temperature of the thermal module. Actual Temperature Shows the set temperature of the thermal module. Actual current Shows the current strength in the thermal module. Time Shows the temperature control duration in min. Remain time Shows the time remaining until the end of temperature control (in min). Temp reached after Shows the time required to reach the set temperature. New Temperature Set temperature ( 5 C to 110 C) New time Temperature control duration (0 to 99 min)

121 epblue Administration 121 The current status is shown in the State area. You can make settings in the Set Temperature area. 1. Enter the set temperature in the New Temperature field. 2. Enter the test duration in the New time field. 3. Click on the Start button. The thermal module heats or cools until the set temperature has been reached or the set time has elapsed. If the set temperature is reached before the set time has elapsed, the thermal module maintains the set temperature until the end of the test. 4. To update the display during the test, press the Refresh button. 5. To activate the fan, press the Fan On button Levelsensor options The Levelsensor options function is used to define the functions the optical sensor will execute. This setting is used as the default. The time for the application is reduced when the optical sensor is switched off. The volume must be entered when the application is started. Abb. 11-4: Levelsensor options function Fig. 11-4: Levelsensor options function Detect volumes radio button Use required minimum volumes radio button The optical sensor measures the filling volume. It is determined in the properties of the labware, in which positions the optical sensor measures the filling volume. epblue assumes that all vessels are filled with the minimum required volume for this run.

122 122 epblue Administration Input volumes manually radio button Slider Detect tips Check labware placement slider Enter filling volume manually. The optical sensor checks, in which positions of the labware pipette tips are available. The optical sensor checks if labware is positioned on the worktable. Information on the optical sensor (see Optical sensor on p. 77) ThermoMixer This function enables you to check the Eppendorf ThermoMixer module. NOTICE! Contamination of samples and the device due to excessive speeds. If the speed is set too high, liquid sprays out of the vessels. Labware may become loose from the holder and fly around. Observe the labware maximum speeds. Tab. 11-1: Maximum speed for labware on the Eppendorf ThermoMixer module Labware Maximum speed in rpm Vessels 1000 Deepwell plates with 96 wells 1200 Deepwell plates with 384 wells 1200 Microplate with 6 wells 1000 Microplates with 96 wells 2000 Microplates with 384 wells 2000 PCR plate with 96 wells 2000 PCR plate with 384 wells 2000 ReservoirRack Mixing not possible Thermorack or Thermoblock 1000 Thermoblock 96 with a semi-skirted PCR plate for 250 μl 1700 Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/V 1600 Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/U 1600 Thermoblock PCR 96 OC 1700 Thermoadapter for 96 wells 1000 Thermoadapter for 384 wells 1000 Thermorack TMX 1300 PrepRack 1300

123 epblue Administration Checking the mixing function of the Eppendorf ThermoMixer module Abb. 11-5: Mixer Control Thermomixer function Fig. 11-5: Mixer Control Thermomixer function RPM field Speed of the Eppendorf ThermoMixer modules in rpm. Time field Duration of the mixing process. Remaining time field Remaining time in the mixing process. Start button Stop button 1. Define the speed and temperature in the Parameter area. 2. To check the mixing function, press the Start button. The Eppendorf ThermoMixer module mixes until the time has elapsed or until the test has been exited using the Stop button.

124 124 epblue Administration Checking the temperature control of the Eppendorf ThermoMixer module Abb. 11-6: Thermomixer temperature control function Fig. 11-6: Thermomixer temperature control function Desired Temperature field Set temperature of the Eppendorf ThermoMixer module. Actual Temperature field Actual temperature of the Eppendorf ThermoMixer module. Actual current field Current strength via the thermal module. Time field Mixing time in min. Remain time field Time until the end of the mixing process (in min). Temp reached after field Time until the set temperature is reached. New Temperature field Enter a new set temperature. 4 C 95 C range. New time field Enter a new mixing time. 0 s - 99 min range. Start button Start the test. Fan on button Switch on the fan. The current status is shown in the State area. You can make settings in the Set Temperature area. 1. Enter the set temperature in the New Temperature field. 2. Enter the mixing time in the New time field. 3. Press the Start button. The Eppendorf ThermoMixer module tempers the labware to the set temperature. If the temperature is reached before the mixing time has elapsed, the Eppendorf ThermoMixer module maintains a constant temperature until the end of the test.

125 epblue Administration Magnetic separation The Magnetic Separation function can be used to check the magnetic finger module. Abb. 11-7: Magnetic Separation function Fig. 11-7: Magnetic Separation function Home Move the magnetic finger module to the home position. Draw In Draw in the magnetic fingers. Draw Out Draw out the magnetic fingers.