1 DNA processing description form INGI Carlantino Project 1.1 Name of the study Complete name Carlantino Project Acronym 1.2 Contact Name Paolo Gasparini Institution University of Trieste, IRCCS Burlo Garofolo Address Telephone +39 040 3785275 Email gasparini@burlo.trieste.it 2 General information 2.1 Sample and DNA handling Please find attached an excel table where details about sample storage,, DNA storage, DNA quantitation, and DNA quality (see table attached below) control are asked. When possible can you provide requested details and send us the relevant protocols?
DNA details table INGI Carlantino Project Main laboratory in charge [ ] RFID Radio Frequency Identification [ ] Barcode, specify 1D [ ], 2D [ ] Sample labels/traceability (% of samples) [ ] Printed labels [ ] Handwritten numbers [ ] Other, specify [ ] [ ] Fresh, specifcy T C [ ], [ ] Refrigerated, specifcy T C [ ], [X] Frozen, Specify T C [ 20] Substrate storage temperatures [ ] Dried (paper) Pre extraction [ ] Liquid Nitrogen, specify dry, gas[ ], liquid sample storage [ ] Others, Specify [ ] format Short term, Substrate storage period Long term [ ] Protocols attached [X] Salting out [ ] Phenol chloroform [ ] Columnary extraction [X] Magnetic beads [ ] Oragene (saliva) method [X] Automated Automation of handling [X] Manual DNA handling and storage Blood Buccal cells Saliva Cell Lines Others http://catalog.p3gobservatory.org/services/files/34f5a04e 4175 4a95 8146 Protocols attached acdc16f6eb44 [X] TE DNA Diluent [X] H2O DNA Concentration [ ] 4 C, specify time [ ] [X] 20 C, specify time [ ] [ ] 40 C, specify time [ ] Storage Conditions [ ] 80 C, specify time [ ], specify time [ ] [ ] Dried [ ]yes [ ]no specify time [ ] Protocols attached Pagina 1
DNA quantification (e.g.: A260/A280, Picogreen) Technical quality Protocols attached [ ] A260/A280 [ ] Picogreen [X] Nanodrop [X] Other [0.8% Agarose gel MarkerIII by Roche] [ ] Automated [ ] Manual [ ] Reproducibility Percentage of samples that are tested DNA Quality control [X] Agarose gel [ ] PCR DNA quality method [ ] A260/A280 ratio Protocols attached Pagina 2
DNA details table INGI Carlantino Project Main laboratory in charge [ ] RFID Radio Frequency Identification [ ] Barcode, specify 1D [ ], 2D [ ] Sample labels/traceability (% of samples) [ ] Printed labels [ ] Handwritten numbers [ ] Other, specify [ ] [ ] Fresh, specifcy T C [ ], [ ] Refrigerated, specifcy T C [ ], [X] Frozen, Specify T C [ 20] Substrate storage temperatures [ ] Dried (paper) Pre extraction [ ] Liquid Nitrogen, specify dry, gas[ ], liquid sample storage [ ] Others, Specify [ ] format Short term, Substrate storage period Long term [ ] Protocols attached [X] Salting out [ ] Phenol chloroform [ ] Columnary extraction [X] Magnetic beads [ ] Oragene (saliva) method [X] Automated Automation of handling [X] Manual DNA handling and storage Blood Buccal cells Saliva Cell Lines Others http://catalog.p3gobservatory.org/services/files/34f5a04e 4175 4a95 8146 Protocols attached acdc16f6eb44 [X] TE DNA Diluent [X] H2O DNA Concentration [ ] 4 C, specify time [ ] [X] 20 C, specify time [ ] [ ] 40 C, specify time [ ] Storage Conditions [ ] 80 C, specify time [ ], specify time [ ] [ ] Dried [ ]yes [ ]no specify time [ ] Protocols attached Pagina 1
DNA quantification (e.g.: A260/A280, Picogreen) Technical quality Protocols attached [ ] A260/A280 [ ] Picogreen [X] Nanodrop [X] Other [0.8% Agarose gel MarkerIII by Roche] [ ] Automated [ ] Manual [ ] Reproducibility Percentage of samples that are tested DNA Quality control [X] Agarose gel [ ] PCR DNA quality method [ ] A260/A280 ratio Protocols attached Pagina 2