15 SERIES SOLVENT DELIVERY SYSTEMS Are you challenged with? Poor chromatographic reproducibility and precision System downtime resulting in sample backlogs Limited flow rate range Excessively long cycle times Lack of sensitivity As your lab s productivity demands increase, so do your expectations for the reliability, performance and flexibility of your solvent delivery system. Waters 15 Series HPLC pumps have been designed to meet all of your flow range needs from low flow to semi-preparative. Exceptional flow rate across all ranges provides you with precision and accuracy, delivering the chromatographic reproducibility that you need to fully automate all your processes. With exceptional solvent blending and pulse-free delivery, the Waters 15 Series HPLC pumps can meet your most sensitive application challenges. Waters 1525 Pump with the 717plus Autosampler, 2487 Dual Wavelength Detector and Empower Software.
Waters offers a complete line of affordable, high-performance binary and isocratic pumps that are both easy-touse and reliable. The 1525, 1525µ and 1525EF are integrated, high-pressure, binary HPLC pumps that feature on-board pulse dampening and efficient mixing. Their pulse-free solvent flow makes then the perfect choice for the use with highly sensitive detectors. The 1525 Binary HPLC Pump is ideal for analytical flows, while the 1525µ Binary HPLC pump is ideal for LC/MS and micro applications at low flow rates, and the 1525EF Binary HPLC pump is designed for semipreparative applications. The 1515 HPLC pump is the isocratic version of the 1525 Binary HPLC Pump. 1515 Isocratic/1525 Binary HPLC Pump: µl pump heads Optional manual injector Optional integral vacuum degasser Optional automated seal wash For routine HPLC applications; 2-4.6 mm ID column separations 1525µ Binary HPLC Pump: 5 µl pump heads Low delay volume < µl with mixer, <3 µl without mixer Integral vacuum degasser Optional automated seal wash For micro applications; 1-2 mm ID column separations 1525EF Binary HPLC Pump: 225 µl pump heads Optional FlexInject manual dual injector module for both analytical and semi-preparative injections Optional integral vacuum degasser Optional automated seal wash For semi-preparative applications; 1-19 mm ID column separations 15 Series Column Heater: Accessory for any 15 series pump Temperature range of ambient to 6 C
Waters 15 Series HPLC Systems controlled by Waters Chromatography Data Software Due to their flow rate delivery accuracy, reliability and reproducibility, the Waters 15 Series HPLC Pump combined with Waters Sample Manager, Waters Detector and Waters Chromatography Data Software, which includes Empower and Breeze software, is a perfect fit for any user looking for a modular system to fit their laboratory needs. The 15 Series HPLC Pumps, combined with Empower or Breeze Software, allows users to be more productive in running separations and analyzing results. With the simple to use and learn Breeze software, operation of the 15 Series HPLC Pumps is done in just a few clicks. With Empower Software control of your 15 Series HPLC Pumps, you can just as easily run your 15 series LC system in the QuickStart interface. Common applications that use the 15 series HPLC Pumps include; QA/QC labs, food and beverage applications, and natural products. AU.26.24.22.2.18.16.14.12.1.8.6.4.2. Pump: 1525 Binary HPLC Pump Injector: 717plus, low volume kit Flow Rate: 2. ml/min Mobile Phase: Water, methanol and glacial acetic acid (69:28:3) Detector: 2487 Dual Wavelength at 275 nm Column: 4.6 mm X mm Symmetry C 18, maintained at 45 ± 1 C..5 1. 1.5 2. 2.5 3. 3.5 4. 4.5 5. 5.5 6. Minutes Figure 1: The overlay of 8 injections demonstrates a consistent retention time performance of the Waters 1525 Binary HPLC Pump.
Waters 15 Series LC/MS and LC/MS/MS Systems controlled by MassLynx Software Because of its low delay volume and reproducibility across all flow ranges, the Waters 15 Series Binary HPLC Pumps are ideally suited to be an inlet to mass detectors such as the ZQ mass spectrometer and Quattro micro mass analyzer. The highly reproducible flow rates make the 15 Series HPLC Binary Pumps compatible with Atmospheric Pressure Ionization (API) sources commonly used in mass spectrometry. The 15 Series Binary HPLC Pumps are fully controlled by MassLynx Software. The MassLynx user interface makes configuring the 15 Series HPLC Binary Pumps intuitive. The sample-centric approach of MassLynx software allows anyone to quickly learn and utilize LC/MS and LC/MS/MS. Common MS applications that use the 15 Series Binary HPLC Pumps as their front end include Early Candidate Profiling, ADME Screening, and Compound Confirmation and Identification. Sample: Atenolol, pindolol, valethamate, propranolol and protriptyline (in retention order), each 1 ng/ml, prepared in 33 MeOH Pump: 1525µ Binary HPLC Pump Injector: 2777 Sample Manager Injection Volume: 1 µl Temperature: 5 C Flow Rate:.4 ml/min Mobile Phase: A: 1 mm NH4HCO3, ph 1 B: MeOH with 1 mm NH 4HCO3, ph1 Gradient: 5-95 B over 3 minutes MS Detection: Waters Micromass Quattro Ultima ESI+ with MRM data monitoring Column: XTerra MS C18 2.1 x 2 mm IS, 3.5 µm 1.64 1.64 2.5 2.53 2.53.5 1. 1.5 2. 2.5 3. 3.5 4. 4.5 2.62 2.78 2.78 36.1 > 218.9 7.72e5 266.9 > 144.9 2.1e5 264 > 191.1 1.36e6 259.9 > 183.1 5.24e5 248.9 > 172 4.17e4 4.37 TIC 1.38e6 Time Figure 2. Due to its low delay volume and accurate flow rate delivery the 1525µ Binary HPLC Pump is the optimal choice for an inlet to LC/MS/MS that demand high throughput. Waters system with the 1525µ Binary HPLC Pump, 2777 Sample Manager, 2996 PDA Detector, Quattro micro Mass Spectrometer and MassLynx Software.
Low friction bearings for reliability Lifetime maintenance-free lubrication Pump heads New, self-aligning mechanical design for longer seal life Non-circular drive gears provide smooth, uniform eluent delivery Stepper motor Waters 15 Series Solvent Delivery Systems utilize a simple mechanical design that has proven reliability. Precise stepper motor control and dual reciprocating pistons provide pulse-fee solvent delivery. The 15 Series HPLC Pumps are built for high performance, reliability, reproducibility and accuracy. With choice of Isocratic or Gradient pumps, a dynamic flow rate range specific to each pump and a maximum operating pressure of up to 6 psi, the 15 Series HPLC Pumps can accommodate all of your HPLC needs. The 15 Series HPLC Pump options include a 15 Series Column heater, an integral vacuum degasser and an automated seal wash. The column heater maintains an accurate and consistent column temperature, which is important when comparing method runs in various labs. An integral vacuum degasser removes dissolved gases from the mobile phase improving performance and reliability. (The integral vacuum degasser is standard in the 1525µ Binary HPLC Pump.) Finally, an automated seal wash helps to extend the primary seal life for reliable operation.
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