CURRICULUM VITAE Personal Details Name: Ana Lúcia Fernandes Pereira e-mail: ana.lpereira@gmail.com Date of Birth: 25 th November 1981 Employment 2010- Postdoctoral Fellow Chromosome Instability & Dynamics Lab., Instituto de Biologia Molecular e Celular (IBMC), Porto, Portugal Methods in Animal Experimentation (Mice) Mammalian Cell Culture (cell lines, primary cells) Brightfield and Confocal Microscopy Animal Embryology and Physiology expertise Animal Tissue Preparation for Microscopic Analysis Education 2006-2010 Ph.D. in Human Biology Faculdade de Medicina da Universidade do Porto, Porto, Portugal 2006-2009 Ph.D. student (January 2009-January 2010): Chromosome Instability & Dynamics Lab., Instituto de Biologia Molecular e Celular (IBMC), Porto, Portugal Ph.D. student (February 2006 - December 2008): Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands Project: Generation and functional analysis of Clasp KO mice (Supervisor: Dr. Helder Maiato; Co-supervisor: Dr. Niels Galjart ) Methods in Animal Experimentation (Mice) Animal Physiology expertise Mammalian cell culture (cell lines, primary cells), cell transfection, production of lentivirus, cell transduction. Molecular Biology Techniques: cloning, PCR, southern blot. Biochemistry : western blot, protein expression and purification.
2005 Undergraduate student/fellowship: Chromosome Instability & Dynamics Lab., Instituto de Biologia Molecular e Celular (IBMC), Porto, Portugal Project: Dissecção Molecular In Vivo das Vias Morfogenéticas do Fuso Mitótico Implicações para a Aneuploidia e o Cancro. (Supervisor: Dr. Helder Maiato) Cell Biology : mammalian cell culture (cell lines, primary cells), immunofluorescence Optical Microscopy: live-cell imaging, fluorescence microscopy. 2003-2004 Undergraduated internship: Instituto de Biologia Molecular e Celular (IBMC), Porto, Portugal; Faculdade de Ciências da Universidade do Porto (FCUP), Poro, Portugal Project: Selecção de compostos com interesse terapêutico nas amiloidoses hereditárias associadas à transtirretina (TTR) estudo do efeito estabilizador no tetrâmero da TTR. Unidade de Neurobiologia Molecular (IBMC) and Departamento de Química da Faculdade de Ciências (FCUP) (Supervisors: Doutora Rosário Almeida and Doutora Margarida Bastos) Biochemistry: production and purification of recombinant proteins, competition assays of a radioactive compound, bi-dimensional analysis, isoelectric focusing Methods of isoelectric calorimetry 1999-2004 Biochemistry degree (Licenciatura) Faculdade de Ciências da Universidade do Porto (FCUP), Portugal / Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Porto, Portugal Certificates Obtained Radiation Protection Course (level 5b) Erasmus Medical Centre, Rotterdam, The Netherlands, June 2005 (local certificate, giving the right to perform experiments with radioactivity at the Erasmus MC) International Course on Laboratory Animal Science Department of Laboratory Animal Science, Utrecht University, The Netherlands, June 2004 ( Artikel 9 certificate, giving the right to do experiments with laboratory animals, equivalent to FELASA cat.c)
Communications Poster presentations: Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Robbert Rottier, Niels Galjart and Helder Maiato. CLASP1 is essential for neonatal lung function and survival. I3S Scientific Retreat, Póvoa de Varzim, Portugal, May 2014 for neonatal lung fuction and survival. ASCB Meeting, San Francisco, CA, USA, December 2012 for neonatal lung fuction and survival. I3S Scientific Retreat, Póvoa de Varzim, Portugal, May 2012 Ana Lúcia Pereira, Luísa Ferreira, Martine Jaegle, Niels Galjart and Helder Maiato. CLASP1 is an essential protein for life after birth. I3S Scientific Retreat, Póvoa de Varzim, Portugal, May 2011 for murine Viability. Aneuploidy Meeting, Edinburgh, Scottland, June 2010 for murine Viability. I3S Scientific Retreat, Póvoa de Varzim, Portugal, May 2010 Ana L. Pereira, António J. Pereira, Ana R.R. Maia, Ksenija Drabek, C. Laura Sayas, Polla J. Hergerts, Mariana Lince-Faria, Irina Matos, Cristina Duque, Tatiana Stepanova, Conly L. Rieders, William Earnshaw, Niels Galjart and Helder Maiato. Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and Kinetochore function. ASCB Meeting, San Diogo,CA, USA, December 2006 Oral communications (Invited Lecturer/Invited Speaker) Master Course in Molecular and Cell Biology, "Cell Division: the role of CLASPs in mammalian development and aneuploidy prevention, Faculdade de Ciências da Universidade do Porto, (2015) 7th Course on Optical Microscopy Imaging for Biosciences, "Tissue preparation for Microscopic Analysis", Porto, Portugal (2015) 6th Course on Optical Microscopy Imaging for Biosciences, "Tissue preparation for Microscopic Analysis", Porto, Portugal (2014) XVI Portugaliae Genetica, "From cells to animals: CLASPing the bigger picture", Porto, Portugal, 2014 Master Course in Molecular and Cell Biology, "Cell Division: the role of CLASPs in mammalian development and aneuploidy prevention, Faculdade de Ciências da Universidade do Porto, (2014) Biochemistry Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da Universidade do Porto, (2014) Biology Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da Universidade do Porto, (2014) 5th Course on Optical Microscopy Imaging for Biosciences, "Tissue preparation for Microscopic Analysis", Porto, Portugal (2013)
Biology Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da Universidade do Porto, (2013) Biochemistry Course, "CLASPs in Cell Division and Aneuploidy Prevention", Faculdade de Ciências da Universidade do Porto, (2013) Postoc Seminar Series, "Clasp1 is essential for neonatal lung function and survival", Instituto de Biologia Molecular e Celular, Porto, Portugal (2012) Master Course in Molecular and Cell Biology, "Cell Division: the role of CLASPs in aneuploidy prevention, Faculdade de Ciências da Universidade do Porto, (2012) Master Course in Diagnóstico e Terapêutica (Diagnosis and Therapeutics), "Afonso Clasp1: da geração à análise funcional de um modelo knockout", Escola Superior de Tecnologia da Saúde do Porto, Portugal (2012) Conferences attended 18th MGC Symposium, Rotterdam, September 2008 European Career Event in Molecular Cell Biology and Medicine, June 2007, Porto, Portugal 17th MGC Symposium, Rotterdam, September 2007 Student Supervision Co-Supervisor of Ana LuísaFerreira during her Master Course - Master in Tecnologia Bioquímica em Saúde (Biochemical Technology in Health), Escola Superior de Tecnologia da Saúde do Porto, Instituto Politécnico do Porto, Porto, Portugal) Publications Scientific Research Microtubule detyrosination guides chromosomes during mitosis Barisic M., Silva e Sousa R., Tripathy S.K., Magiera M.M., Zaytsev A.V., Pereira A.L., Janke C., Grishchuk E.L., Maiato H. (2015) Science. 15;348(6236):799-803 The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem cell maintenance Drabek K., Gutiérrez L., Vermeij M., Clapes T., Patel S.R., Boisset J.C., van Haren J., Pereira A.L., Liu Z., Akinci U., Nikolic T., van Ijcken W., van den Hout M., Meinders M., Melo C., Sambade C., Drabek D., Hendriks R.W., Philipsen S., Mommaas M., Grosveld F., Maiato H., Italiano J.E. Jr, Robin C., Galjart N.(2012). Cell Rep. 2(4):781-8
Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and poleward flux Maffini S., Maia A.R.R., Manning A.L., Maliga Z., Pereira A.L., Junqueira M., Shevchenko A. Hyman A., Yates J.R. 3rd, Galjart N., Compton D.A. and Maiato H. (2009), Curr Biol 19(18):1566-72 Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and Kinetochore function Ana L. Pereira, António J. Pereira, Ana R.R. Maia, Ksenija Drabek, C. Laura Sayas, Polla J. Hergerts, Mariana Lince-Faria, Irina Matos, Cristina Duque, Tatiana Stepanova, Conly L. Rieders, William Earnshaw, Niels Galjart and Helder Maiato (2006), Molecular Biology of the Cell 17, 4526-4542 New designs for MRI contrast agents P.A. Fernandes, A.T.P. Carvalho, A.T. Marques, A.L.F. Pereira, A.P.S. Madeira, A.S.P. Ribeiro, A.F.R. Carvalho, E.T.A. Ricardo, F.J.V. Pinto, H.A. Santos, H.D.G. Mangericão, H.M. Martins, H.D.B. Pinto, H.R.R. Santos, I.S. Moreira, M.J.V. Azeredo, R.P.S. Abreu, R.M.S. Oliveira, S.F.M. Sousa, R.J.A.M. Silva, Z.S. Mourão & M.J. Ramos (2003), J Comput Aided Mol Des 17, 463-473 Reviews and Book Chapters Microtubule plus-end tracking proteins. els, DOI:10.1002/9780470015902.a0025979 Pereira, A.L. and Maiato,H. Microtubule plus-end tracking proteins and their role in cell division Ferreira, J.G., Pereira, A.L., Maiato, H.; Int Rev Cell Mol Biol. 2014;309:59-140. Thesis Generation and Functional Analysis of Clasp1 Knockout Mice Ph.D. Thesis, 2009 Ana Lúcia Fernandes Pereira