Europass Curriculum Vitae Personal information First name(s) / Surname(s) Address(es) Telephone(s) Fax(es) E-mail Nationality Greta Maria Paola / Giordano Attianese 11, Vy D Etrà, 2000,Neuchatel, (NE), Switzerland +41(0)21 692 41 39 (lab) +41(0)766506972 (mobile) +41(0)21 692 41 15 (Secretary Office) Greta.GiordanoAttianese@unil.ch (Lab) gretaroses@gmail.com (Personal) Italian Date of birth 06/11/1981 Gender Female Work experience From October 2011-to now Post Doctoral Scholar Analysis of epigenetic changes in white adipose tissue aging. PPAR beta molecular mechanism via global approach. Occupation or position held Center For Integrative Genomics (CIG), Genopode Building, University of Lausanne. Supervisor: Prof. Beatrice Desvergne Basic Science Research (Metabolism); From October 2010-April 2011 Post Doctoral Fellow (acquisition of Transposon mediated gene transfer technique) Transposon mediated gene transfer Supervisor Prof. Andrea Biondi and Dr. Ettore Biagi Centro Ricerca M.Tettamanti, Clinica Pediatrica, Università degli Studi di Milano-Bicocca, Ospedale S. Gerardo, Monza, Italy, in agreement with Dr.Laurence Cooper, M.D.Anderson Cancer Center, Houston, Texas. Applied Science Research (Immunotherapy) From May 2010-to September 2011 Post Doctoral Fellow Functional characterization of CD23 specific chimeric receptor transduced T-cell in vivo Prof. Andrea Biondi and Dr. Ettore Biagi Centro Ricerca M. Tettamanti, Clinica Pediatrica, Università degli Studi di Milano-Bicocca, Ospedale S. Gerardo, Monza, Italy, in agreement with Università Vita-Salute San Raffaele, Milano, Italy Page 1/7 - Curriculum vitae of Giordano
Applied Science Research (Immunotherapy); Research Fellowship of Università Milano-Bicocca January 2007- May 2010 PhD student in Molecular Medicine- Section of Basic and Applied in I immunology Generation of anti-cd23 chimeric receptor as a new immunotherapeutic approach for B-Chronic Lymphocytic leukemia Name and address of employer Occupation or position held Prof. Andrea Biondi and Dr. Ettore Biagi, second supervisor Dr.Roberto Giovannoni Centro Ricerca M. Tettamanti, Clinica Pediatrica, Università degli Studi di Milano-Bicocca, Ospedale S. Gerardo, Monza, Italy, in agreement with Università Vita-Salute San Raffaele, Milano, Italy Applied Science Research (Immunotherapy); Fellowship of Università Vita-Salute San Raffaele September 2005 September 2006 Master Student with scholarship Pre-PhD stage abroad Supervisor Prof. Giampietro Dotti and Dr.Ettore Biagi; Dr.Andrea Biondi, CAGT Baylor College of Medicine, Texas Children Hospital 6621 Fannin St. Houston, TX 77030 (USA) in agreement with Centro Ricerca M.Tettamanti, Clinica Pediatrica, Università degli Studi di Milano Bicocca, Ospedale S.Gerardo, Monza, Italy Applied Science Research (Immunotherapy); Fellowship of Fondazione M.Tettamanti September 2004 July 2005 Master Student Molecular and functional analysis of fusion gene PAX5/ETV6, originated from t(9;12)(q11;p13) Prof. Andrea Biondi, second supervisor Dr.Grazia Fazio, Centro Ricerca M. Tettamanti, Clinica Pediatrica, Università degli Studi di Milano-Bicocca, Ospedale S. Gerardo, Monza, Italy Internship for Master of Science experimental thesis (second level degree) Page 2/7 - Curriculum vitae of Giordano
Education and training 31st May 2010 (2007-2010) PhD in Molecular Medicine- Section of Basic and Applied Immunology Generation of anti-cd23 chimeric receptor as a new immunotherapeutic approach for B-Chronic Lymphocytic leukemia Università Vita-Salute San Raffaele, Milano, Italy 15th July 2005 (2003-2005) Doctor in Medical Biotechnology, second level university degree (Master degree) Molecular and functional analysis of fusion gene PAX5/ ETV6, originated from t(9;12)(q11;p13). Facoltà di Medicina e Chirurgia Università degli Studi di Milano-Bicocca Score of 110/110 magna cum laude 6th November 2003 (2000-2003) Doctor in Sanitary Biotechnology, First level university degree (Bachelor degree) Protein and peptides analysis by mass spectrometry techniques Facoltà di Scienze chimiche, fisiche e matematiche Università degli Studi di Milano-Bicocca Score of 110/110 25th July 2000 (1996-2000) High School Diploma Diploma di maturità Liceo Classico, linguistic curriculum Liceo Classico Statale Ettore Majorana, Desio, Italy Score of 93/100 Page 3/7 - Curriculum vitae of Giordano
Prize and Fellowship 2010 Travel Award SIES (XI Congresso Nazionale della società italiana di ematologia sperimentale Torino-Centro congress Lingotto, 6-8th October 2010) 2010 Research Fellowship Analisi del fenotipo di cellule leucemiche e/o di cellule effettrici per approcci innovative di trapianto di cellule staminali emopoietiche e di immunoterapia adottiva, Università Milano-Bicocca 2008 Travel Award ASH 50th (50th Annual Congress American Society of Haematology ASH San Francisco- USA, 6-9 th December 2008 2008 Travel Award SIES (X Congresso Nazionale della società italiana di ematologia sperimentale Bari-Hotel Sheraton Nicolaus, 24-26th September 2008) 2007 Fellowship of PHD Program in Molecular Medicine Application in Immunology Università Vita Salute S.Raffaele 2003 Fellowship of Bracco Imaging S.p.A. Page 4/7 - Curriculum vitae of Giordano
Scientific publications (See attached document List of Publications ) Personal skills and competences Mother tongue(s) Other language(s) Italian English, with approved examination in scientific English at Università degli Studi di Milano-Bicocca, Italy French Self-assessment Understanding Speaking Writing European level (*) Listening Reading Spoken interaction Spoken production English C1 C1 C1 C1 C1 French B1 B1 C1 C1 A1 (*) Common European Framework of Reference for Languages Social skills and competences Good relational and social skills; attitude to present in international meetings. Highly goal oriented. Decisional autonomy Page 5/7 - Curriculum vitae of Giordano
Organisational skills and competences Technical skills and competences Short and long term work independent planning; students work training; teaching; Isolation of mononuclear cells from peripheral blood, bone marrow and cord blood by Ficoll density gradient Cells purification by immunomagnetic selection Culture of cell lines and primary cells Culture of human B-CLL primary cells Citokine Induced Killer cells (CIK) generation OKT3/CD28 in vitro simulated T cells generation Citotoxicity and Proliferation assay (with 51chromium and 3H-timidine) Flow cytometry (assays and data analysis) Transient transfection methods Production of retroviral vectors and transduction of cell lines and primary cells Transposon mediated gene transfer (Sleeping Beauty System) Transformation of bacteria Purification of plasmidic DNA Cloning design Synthetic DNA technology DNA restriction analysis RNA and DNA isolation RT- PCR Q-PCR (Sybr Green) Protein extraction Immunoprecipitation and Western Blot analysis ELISA Chromatin preparation (from different mouse tissue) Chromatin-Immunoprecipitation (from different mouse tissue) Computer skills and competences Artistic skills and competences Operative systems: Windows 2000, XP (Microsoft Office: Word, Power Point, Excel). Internet softwares: Microsoft Explorer; Firefox Internet applications: biological and scientific databases (NCBI, BLAST, SWISSPROT, CLUSTAL-W) Applicative softwares: Cell Quest for FACS analysis, Sequence Detection System software Fiction writer, vocalist and lyric writer, cartoonist Driving licence Italian B driver licence Page 6/7 - Curriculum vitae of Giordano
Formative experience Tutor for Power Point, Excel, Access first level scholastic course at Scuole superiori CIOFS Cesano Maderno (2000) Additional information Attached document 1: List of pubblication Lausanne, 23 rd September 2014 Page 7/7 - Curriculum vitae of Giordano