Fantatstic Winter Offers From Roche Applied Science

Fantatstic Winter Offers From Roche Applied Science Come in from the cold this winter and take 50% off the list price of 100 s of products from our extensive range of Enzymes for PCR and Restriction Enzymes. These offers are valid from 1 st October 2008 until 19 th December 2008. Roche Applied Science provides an extensive portfolio of Enzymes for PCR to meet all your amplifaction needs. For further details of these products or for help identifying the best products for your project please visit our NEW website at www.rocheapplied-science.com. To take advantage of these heart warming offers, Simply send an email quoting your contact details, the catalogue numbers and quantities of the items that you require to: Tooran.shahbazi@roche.com A special quotation number will be emailed to you the same day. Place your order by telephone, fax, or email stating your unique quotation number. FREEphone 0808 100 99 98 FREEfax 0808 100 80 60 email: burgesshill.ras@roche.com Terms and Conditions: Offer valid on orders received by telephone, fax or email from the 01/10/08 18/12/08. Only orders clearly stating a valid quotation number are eligible to receive discount. Quotation number can not be applied retrospectively to an order. Online orders placed via the Roche Applied Science website are excluded from this promotion. Roche Applied Science may, at its sole discretion, terminate this offer at any time. For Full Terms and Conditions please visit www.roche-applied-science.com Roche Diagnostics Ltd Charles Avenue Burgess Hill, RH15 9RY Reg: 571546

Enzymes, Reagents and Kits for PCR Description Expand Cloning Kit 1 Kit (10 x Rxns, incl. 1 11940392001 419 209.50 control) Human Genomic DNA 100µg 11691112001 107.40 53.70 Human tpa Control Primer Set 1 Set 11691104001 175.30 87.65 MgCl 2 Stock Solution 3 x 1ml 11699113001 22 11 PCR Buffer Set 2 x 2ml (2 x 1ml of each) 11699121001 26.20 13.10 PCR Buffer without MgCl 2, 10 x 3 x 1ml 11699105001 22 11 PCR Cloning Kit (blunt end) 1 kit (35 x Rxns & 5 11939645001 306.10 153.05 contol) PCR Core Kit 1 Kit (100 rxns, 50µl 11578553001 241.20 120.60 final vol.) PCR Core Kit PLUS rxns, 50µl 11585541001 143.50 71.75 1 Kit (50 final vol.) PCR ELISA (DIG Detection) 1 kit 11965409910 475.30 237.65 PCR ELISA (Dig Detection) 1 Kit (192 detection 11636111910 266.10 133.05 rxns) PCR ELISA (Dig labeling) 1 Kit (50 rxns) 11636120910 315.40 157.70 PCR Master 1 Kit (200 PCR rxns, 50µl final 11636103001 234.20 117.10 vol.) PCR Optimization Kit 1 Kit 11636138001 150 75 Pwo Master 2.5ml (10 x 250µl)(100 rxns 50µl 03789403001 143.30 71.65 final vol.) Rapid DNA Ligation Kit 1 Kit (40 DNA ligation rxns) 11635379001 103.80 51.90 Uracil-DNA Glycosylase 100U 11444646001 92.50 46.25 Uracil-DNA Glycosylase (Heatlabile) 100U 11775367001 98.80 49.40 Uracil-DNA Glycosylase (Heatlabile) 500U 11775375001 362.40 181.20 Water, PCR Grade 25ml (1x25ml) 03315959001 33.70 16.85 Water, PCR Grade 100ml (4x25ml) 03315843001 84.60 42.30 Water, PCR Grade 25ml (25x1ml) 03315932001 42 21

Expand 20kb PLUS PCR 200U 04743814001 111 55.50 Expand HiFi PCR 100U 11732641001 51.50 25.75 Expand HiFi PCR 2,500U 11759078001 1048.50 524.25 Expand HiFi PCR 500U 11732650001 236.40 118.2 Expand HiFi PCR 100 U 04738250001 60 30 Expand HiFi PCR 2,500U 04738276001 1129.10 564.55 Expand HiFi PCR 500U 04738268001 251.20 125.60 Expand HiFi PLUS PCR 125U 03300242001 65 32.50 Expand HiFi PLUS 2,500U PCR 03300234001 1127 563.50 Expand HiFi PLUS 500U PCR 03300226001 254 127 Expand HiFi PLUS PCR 125U 04743725001 75 37.50 Expand HiFi PLUS PCR 500U 500U 04743733001 268 134 Expand Long Range 175U (50 rxns) 04829034001 87 43.50 3,500U Expand Long Range (5 x 700U) 3500 U (1,000 rxns) 04829069001 1490 745 Expand Long Range 700U (200 rxns) 04829042001 331 165.50 Expand Long Template PCR 150U 11681834001 63.80 31.90 Expand Long Template PCR 3,600U (10 x 360U) 11759060001 1355.60 677.80 Expand Long Template PCR 720U (2 x 360U) 11681842001 305.40 152.70 Expand 20kb PLUS PCR 200U 11811002001 95.40 47.70 FastStart HiFi PCR 125U 04738284001 80 40 FastStart HiFi PCR 500U 04738292001 256.50 128.25 FastStart High Fidelity PCR 125U 03553426001 66 33 FastStart High Fidelity PCR 500U 03553400001 242 121 FastStart High Fidelity PCR 2,500U 03553361001 1075 537.50

FastStart PCR Master 10ml (8 x 1.25ml)(400 rxns 50µl 04710444001 194 97 final vol.) FastStart PCR Master 2ml (2 x 1.25ml)(100 rxns 50µl 04710436001 68 34 final vol.) FastStart PCR Master 10 x 5ml (2,000 rxns 50µl final 04710452001 910 455 vol.) FastStart Taq DNA Pol., 100U 04738314001 31 15.50 FastStart Taq DNA Polymerase 1,000U, (4 x 250U) 04738381001 221 110.50 FastStart Taq DNA Polymerase 2,500U, 04738403001 545 272.50 FastStart Taq DNA Pol., 500U 500 U 04738357001 114 57 FastStart Taq DNA Pol., 5,000U 5000 U (20 x 250U) 04738420001 1034 517 FastStart Taq DNA Polymerase, 100U 12032902001 26 13 FastStart Taq DNA Polymerase, 1,000U (4 x 250U) 12032937001 188 94 FastStart Taq DNA Polymerase, 2,500U 12032945001 463 231.50 FastStart Taq DNA Polymerase, 50U 12158264001 17 8.50 FastStart Taq DNA Polymerase, 500U 12032929001 97 48.50 FastStart Taq DNA Polymerase, 5,000U (20 x 250U) 12032953001 879 439.50 GC RICH PCR 100U 12140306001 123.20 61.60 GC Rich PCR 100U 04743784001 136 68 High Fidelity PCR Master 1 Kit (200 rxns 50µl final 12140314001 294.70 147.35 vol.) Pwo DNA Polymerase 100U 11644947001 63.70 31.85 Pwo DNA Polymerase 500U 11644955001 307 153.50 Pwo Superyield DNA Polymerase 100U 04743750001 106 53 Pwo SuperYield DNA Polymerase 100U 04340868001 90 45 Pwo SuperYield DNA Polymerase 500U 04340850001 380.00 190 Taq DNA Polym. GMP Grade, (5 U/µL) 1,000U 03734935001 2000.00 1000

Taq DNA Polym. GMP Grade, (5 U/µL) 1,000U 03734927001 425.00 212.50 Taq DNA Polymerase 2,500U 11596594001 463.00 231.50 Taq DNA Polymerase 5,000U (20x250 U) 11435094001 879.00 439.50 Taq DNA Polymerase (1u/µl) 1,000U (4 x 250U) 04738241001 221.00 110.50 Taq DNA Polymerase (1u/ul) 250 U 250U 04738225001 62.00 31 Taq DNA Polymerase 100U 04728866001 310.00 15.50 Taq DNA Polymerase 1,000U (4 x 250U) 04728882001 221.00 110.50 Taq DNA Polymerase 2,500U 04728904001 545.00 272.50 Taq DNA Polymerase 500U 04728874001 114.00 57 Taq DNA Polymerase 5,000U (20 x 250U) 04728858001 1034.00 517 Taq DNA Polymerase, (1U/µl) 250U 11647679001 59.00 29.50 Taq DNA Polymerase (1U/µl) 1,000U (4x250U) 11647687001 188.00 94 Taq DNA Polymerase 100U 11146165001 26.00 13 Taq DNA Polymerase 500U 11146173001 97.00 48.50 Taq DNA Polymerase 1,000U (4 x 250U) 11418432001 188.00 94

Restriction Enzymes and Buffers Description Buffers SuRE/Cut Buffer A 5 x 1ml 11417959001 13.20 6.60 SuRE/Cut Buffer B 5 x 1ml 11417967001 13.20 6.60 SuRE/Cut Buffer H 5 x 1ml 11417991001 13.20 6.60 SuRE/Cut Buffer L 5 x 1ml 11417975001 13.20 6.60 SuRE/Cut Buffer M 5 x 1ml 11417983001 13.20 6.60 SuRE/Cut Buffer Set for Restr. Enzymes 1 Set 11082035001 14.90 7.45 Restriction Enzymes Meganuclease I-Sce I 1,000U 11362399001 300.10 150.05 Aat II (1-5U/µl) 250U 10775207001 31.00 15.50 Acc I 500U 10728438001 97.00 48.50 Acy I (Aha II) 200U 11081314001 137.40 68.70 Afl III 100U 11209183001 61.00 30.50 Alu I (10U/µl) 2,000U 10656267001 63.80 31.90 Alu I (10U/µl) 500U 10239275001 22.10 11.05 Apa I (40U/µl) 20,000U 10703753001 120.30 60.15 Apa I (10U/µl) 5,000U 10899208001 34.30 17.15 Asp 700 (Xmn I) (10U/µl) 500U 10835277001 37.70 18.85 Asp 718 (10U/µl) 1,000U 10814245001 36.70 18.35 Asp 718 (10U/µl) 5,000U 10814253001 145.90 72.95 Asp 718, conc (40U/µl) 5,000U 11175050001 145.90 72.95 Asp EI (10U/µl) 200U 11428179001 82.60 41.30 Asp I (Tth 111 I) (10U/µl) 400U 11131354001 40.70 20.35 Ava I 1,000U 10740730001 87.00 43.50 Ava II 500U 10740756001 62.60 31.30 Avi II (10U/µl) 200U 11481436001 56.00 28.00 Bam HI (10U/µl) 10,000U 10656275001 37.10 18.55 Bam HI (10U/µl) 1,000U 10220612001 8.00 4.00 Bam HI (10U/µl) 2,500U 10567604001 10.20 5.10 Bam HI, conc. (40U/µl) 10,000U 10798975001 37.10 18.55 Bam HI,conc. (40U/µl) 50,000U 11274031001 143.70 71.85 Bbr PI (Pma CI) (10U/µl) 500U 11168860001 21.40 10.70 Bcl I, (10U/µl) 500U 10693952001 18.00 9.00 Bcl I, conc. (40U/µl) 2,500U 11097059001 82.00 41.00 Bfr I (Afl II) (10U/µl) 500U 11198939001 36.00 18.00 Bgl I, (10U/µl) 1,000U 10404101001 21.50 10.75 Bgl I, conc. (40U/µl) 5,000U 11047604001 84.00 42.00 Bgl II (10U/µl) 2,000U 10567639001 39.00 19.50 Bgl II (10U/µl) 500U 10348767001 20.40 10.20 Bgl II, conc. (40U/µl) 10,000U 11175068001 299.00 149.50 Bgl II, conc. (40U/µl) 2,000U 10899224001 39.00 19.50 Bln I (Avr II) (10U/µl) 200U 11558161001 53.00 26.50 Bln I (Avr II) (10U/µl) 1,000U 11558170001 211.90 105.95

Bpu AI (10U/µl) 200U 11497944001 55.00 27.50 Bse AI (10U/µl) 200U 11417169001 50.00 25.00 Bsi WI (10U/µl) 300U 11388959001 48.30 24.15 Bsi YI (10U/µl) 200U 11388916001 54.40 27.20 Bsm I (10U/µl) 200U 11292307001 45.00 22.50 Bsp LU 11I (10U/µl) 200U 11693743001 62.00 31.00 Bss HII (10U/µl) 200U 11168851001 42.80 21.40 Bst 1107 I (10U/µl) 200U 11378953001 170.60 85.30 Bst EII (10U/µl) 500U 10404233001 16.00 8.00 Bst XI (10U/µl) 250U 11117777001 25.00 12.50 Bst XI (10U/µl) 1,250U 11117785001 105.00 52.50 Cell II (10U/µl) 200U 11449397001 105.90 52.95 Cfo I (Hha I) (10U/µl) 1,000U 10688541001 21.00 10.50 Cla I (10U/µl) 2,500U 10656291001 87.40 43.70 Cla I (10U/µl) 500U 10404217001 21.90 10.95 Cla I, conc. (40U/µl) 2,500U 11092758001 87.40 43.70 Dde I (10U/µl) 1,000U 10835307001 62.30 31.15 Dpn I (10U/µl) 1,000U 10742988001 110.00 55.00 Dpn I (10U/µl) 200U 10742970001 32.00 16.00 Dra I (10U/µl) 5,000U 10827754001 60.30 30.15 Dra II (1-3U/µl) 250U 10843512001 89.50 44.75 Dra III(1-5U/µl) 500U 10843547001 178.70 89.35 Ecl XI (Xma III) (10U/µl) 1,000U 11131397001 202.00 101.00 Eco 47III 100U 11167103001 83.00 41.50 Eco RI (10U/µl) 10,000U 11175084001 19.90 9.95 Eco RI (10U/µl) 5,000U 10703737001 14.60 7.30 Eco RI, conc. (40U/µl) 10,000U 10200310001 19.90 9.95 Eco RI, conc. (40U/µl) 50,000U 10606189001 92.80 46.40 Eco RII (Bst NI) (10U/µl) 200U 11427881001 97.40 48.70 Eco RV (10U/µl) 10,000U 10667153001 76.00 38.00 Eco RV (10U/µl) 2,000U 10667145001 19.00 9.50 Eco RV, conc. (40U/µl) 10,000U 11040197001 76.00 38.00 Fok I (1-5U/µl) 100U 11004816001 49.00 24.50 Hae III (10U/µl) 5,000U 10693944001 63.00 31.50 Hind II (3-10U/µl) 2,500U 10656305001 94.60 47.30 Hind III (10U/µl) 10,000U 10656321001 26.70 13.35 Hind III (10U/µl) 5,000U 10656313001 15.00 7.50 Hind III, conc. (40U/µl) 10,000U 10798983001 26.70 13.35 Hind III, conc. (40U/µl) 50,000U 11274040001 123.30 61.65 Hinf I (10U/µl) 1,000U 10779652001 17.00 8.50 Hinf I (10U/µl) 5,000U 10779679001 82.00 41.00 Hinf I, conc. (40U/µl) 20,000U 11274082001 251.00 125.50 Hpa I, (3-10U/µl) 100U 10380385001 20.00 10.00 Hpa I, (3-10U/µl) 500U 10567647001 66.00 33.00 Hpa II (10U/µl) 5,000U 10656330001 125.00 62.50 Hpa II, conc. (40U/µl) 5,000U 11207598001 125.00 62.50 Ita I (10U/µl) 200U 11497979001 93.30 46.65

Kpn I (40U/µl) 10,000U 10742953001 72.40 36.20 Kpn I (10U/µl) 5,000U 10899186001 51.00 25.50 Ksp I (10U/µl) 1,000U 11117807001 35.00 17.50 Mae I (1-5U/µl) 250U 10822221001 222.20 111.10 Mae II (1-5U/µl) 50U 10862495001 120.00 60.00 Mae III (1-5U/µl) 250U 10822248001 222.20 111.10 Mae III (1-5U/µl) 50U 10822230001 55.60 27.80 Mlu I (10U/µl) 2,500U 10909718001 64.30 32.15 Mlu I (10U/µl) 500U 10909700001 17.00 8.50 Mlu I, conc. (40U/µl) 2,500U 11207601001 64.30 32.15 Mlu NI (10U/µl) 200U 11526430001 52.00 26.00 Mro I (Acc II) (>1-5U/µl) 100U 11102982001 223.40 111.70 Msp I (10U/µl) 5,000U 10633526001 70.00 35.00 Msp I, conc. (40U/µl) 5,000U 11047647001 70.00 35.00 Mun I (10U/µl) 200U 11441337001 18.60 9.30 Mva I (Bst NI) (10U/µl) 5,000U 11288075001 160.40 80.20 Mvn I (Fnu DII) (10U/µl) 200U 11062573001 82.00 41.00 Nae I (10U/µl) 1,000U 10786322001 70.60 35.30 Nar I (10U/µl) 1,000U 11103024001 135.00 67.50 Nco I (10U/µl) 1,000U 10835323001 32.70 16.35 Nco I (10U/µl) 200U 10835315001 8.20 4.10 Nco I, conc. (40U/µl) 1,000U 11047698001 32.70 16.35 Nde I (10U/µl) 1,000U 11040227001 85.20 42.60 Nde I (10U/µl) 200U 11040219001 22.60 11.30 Nde II (Mbo I) 1,000U 11040243001 91.50 45.75 Nhe I (10U/µl) 1,000U 10885851001 35.00 17.50 Nhe I (10U/µl) 200U 10885843001 8.70 4.35 Nhe I, conc. (40U/µl) 1,500U 10885860001 49.00 24.50 Not I (10U/µl) 1,000U 11014714001 74.50 37.25 Not I (10U/µl) 200U 11014706001 18.60 9.30 Not I, conc. (40U/µl) 1,000U 11037668001 74.50 37.25 Nru I (10U/µl) 1,000U 10776777001 81.00 40.50 Nsi I (10U/µl) 1,000U 10909840001 75.00 37.50 Nsi I (10U/µl) 200U 10909831001 16.00 8.00 Nsp I (10U/µl) 200U 11131419001 74.00 37.00 Pin AI, 1000 u 11464850001 169.00 84.50 Pin AI, 200 u 11464841001 35.00 17.50 Pst I (10U/µl) 10,000U 10621633001 30.00 15.00 Pst I (10U/µl) 3,000U 10621625001 13.10 6.55 Pst I, conc. (40U/µl) 10,000U 10798991001 30.00 15.00 Pvu I 100U 10650137001 27.50 13.75 Pvu I 500U 10650129001 108.00 54.00 Pvu II (10U/µl) 1,000U 10642690001 17.00 8.50 Pvu II (10U/µl) 5,000U 10642703001 47.00 23.50 Rca I (Bsp HI) 200U 11467123001 48.00 24.00 Rsa I (10U/µl) 1,000U 10729124001 31.40 15.70 Rsa I (10U/µl) 5,000U 10729132001 141.60 70.80

Rsa I, conc. (40U/µl) 5,000U 11047671001 141.60 70.80 Rsr II (10U/µl) 200U 11292587001 51.50 25.75 Sac I (Sst I) (10U/µl) 1000U 10669792001 18.50 9.25 Sac I (Sst I) (10U/µl) 5,000U 10669806001 64.30 32.15 Sac I, conc. (40U/µl) 5,000U 11047655001 64.30 32.15 Sal I (10U/µl) 2,500U 10567663001 39.00 19.50 Sal I (10U/µl) 500U 10348783001 9.70 4.85 Sal I, conc. (40U/µl) 2,500U 11047612001 39.00 19.50 Sau3A I (1-5U/µl) 500U 10709751001 72.20 36.10 Sca I (10U/µl) 2,500U 10775266001 81.40 40.70 Sca I (10U/µl) 500U 10775258001 20.00 10.00 Sca I,conc. (40U/µl) 5,000U 11207636001 131.40 65.70 Sex AI (10U/µl) 200U 11497995001 103.10 51.55 Sfi I (10U/µl) 1,250U 11288024001 76.00 38.00 Sfi I, conc. (40U/µl) 5,000U 11288059001 160.00 80.00 Sfu I (Asu II) (10U/µl) 2,000U 11243497001 33.60 16.80 Sgr AI (10U/µl) 200U 11277014001 79.00 39.50 Sma I, (10U/µl) 1,000U 10220566001 21.90 10.95 Sma I, (10U/µl) 5,000U 10656348001 87.40 43.70 Sma I, conc. (40U/µl) 5,000U 11047639001 87.40 43.70 Sna BI (10U/µl) (10U/µl) 10997480001 49.00 24.50 Spe I (10U/µl) 200U 11008943001 17.10 8.55 Spe I (10U/µl) 1,000U 11008951001 80.00 40.00 Spe I, conc. (40U/µl) 1,000U 11207644001 80.00 40.00 Sph I (10U/µl) 200U 11026950001 66.30 33.15 Sph I (10U/µl) 2,500U 11026534001 386.40 193.20 Sph I (10U/µl) 500U 10606120001 87.40 43.70 Sph I, conc. (40U/µl) 2,500U 11026542001 386.40 193.20 Ssp I (10U/µl) 1,000U 10972975001 85.00 42.50 Ssp I, conc. (40U/µl) 1,000U 11207652001 85.00 42.50 Stu I (10U/µl) 2,500U 10753360001 103.00 51.50 Stu I (10U/µl) 500U 10753351001 27.00 13.50 Stu I, conc. (40U/µl) 2,500U 11047680001 103.00 51.50 Sty I (10U/µl) 2,000U 11047744001 35.90 17.95 Swa I (10U/µl) 200U 11371517001 70.00 35.00 Taq I (10U/µl) 10,000U 11175114001 120.00 60.00 Taq I (10U/µl) 2,500U 10567671001 30.00 15.00 Tru 9I (10U/µl) 1,000U 11464825001 142.00 71.00 Van 91 I (Pfl MI) 200U 11379275001 51.50 25.75 Xba I (10U/µl) 1,000U 10674257001 12.30 6.15 Xba I (10U/µl) 20,000U 10674273001 163.00 81.50 Xba I (10U/µl) 5,000U 10674265001 43.90 21.95 Xba I, conc. (40U/µl) 20,000U 11047663001 163.00 81.50 Xho I (10U/µl) 5,000U 10899194001 36.10 18.05 Xho I, conc. (40U/µl) 12,500U 10703788001 76.70 38.35 Xho I, conc. (40U/µl) 2,500U 10703770001 23.50 11.75 Xho II (1-5U/µl) 50U 10742929001 52.90 26.45

Description Xma CI (10U/µl) 200U 11743392001 81.00 40.50