Timothy D. Miles Michigan State University Department of Plant Pathology 105 Center for Integrated Plant Systems East Lansing, MI 48824 Professional Preparation: Doctor of Philosophy (in progress), Plant Pathology, Michigan State University, 2006-present Bachelor of Science, Biology and Chemistry, Western Michigan University, 2005 Research Experience: Graduate Research, Michigan State University, East Lansing, MI, 8/06-present Identified several blueberry genes using suppressive subtractive hybridization in the resistant cultivar Elliott that are differentially expressed after inoculation with the fungus Colletotrichum acutatum, and documented their temporal expression. Conducted chemical extractions of fruit of resistant and susceptible blueberry cultivars looking for differences in secondary metabolites in response to inoculation. Laboratory Technician Plant Molecular Biology, Western Michigan University, 1/06-7/06 Utilized tissue culture to screen eef1α t-dna mutants for various phenotypes such as lethality, limited germination, delayed development, reduction in vitality, and loss in reproductive ability. Also constructed an inducible antisense mutant to block eef1α gene expression in Arabidopsis. Research Associate Reproductive Toxicology, MPI Research, Mattawan, MI, 1/06-5/06 Investigated the safety of several pre-clinical drugs and monitored animals for a response. Organized and annotated research on a variety of mammals (mice, rats, rabbits, etc.). Learned and performed several laboratory techniques such as chemical formulation, blood preparation for serological testing and several dosing procedures. Undergraduate Research, Western Michigan University, 6/04-12/05 Learned specific lab techniques such as DNA extraction, RNA extraction, PCR, and RT- PCR. Screened Arabidopsis t-dna mutants in order to study loss of function mutations in the eef1α gene family. Designed primers and conducted RT-PCR on screened t-dna mutants looking for reduced expression in specific members of the eef1α family. Publications: Glass, Benjamin, Miles, Timothy, Sysak Roger, and Annemiek Schilder. Post-plant strategies for management of black root rot related decline in perennial strawberries. Crop Protection (ready for submission). Schilder, Annemiek and Timothy Miles. 2011. First report of blueberry leaf rust caused by Thekopsora minima on Vaccinium corymbosum in Michigan. Plant Disease (accepted). 1
Miles, Timothy, Woelk, Christopher, Rojas, Alejandro, and Annemiek Schilder. 2011. First report of Pythium root rot in blueberry in the United States. Plant Disease (in press). Miles, Timothy, Day, Brad and Annemiek Schilder. 2011. Identification of differentially expressed genes in a resistant blueberry cultivar after infection by Colletotrichum acutatum. Molecular Plant Pathology. DOI: 10.1111/j.1364-3703.2010.00687.x Miles, Timothy and Annemiek Schilder. 2009. First Report of Grape Root Rot caused by Roesleria subterranea on grapes in Michigan. Plant Disease 93:765 Miles, Timothy, Wharton, Phillip, and Annemiek Schilder. 2009. Cytological and chemical evidence for an active resistance response to infection by Colletotrichum acutatum in Elliott blueberries. Acta Horticulturae (ISHS) 810:810:361-368. Miles, Timothy, and Annemiek Schilder. 2009. Correlation of signs and symptoms of mummy berry in highbush blueberry. Acta Horticulturae (ISHS) 810:379-384. Research Abstracts and Presentations: Miles, Timothy, Hancock, James, Callow, Pete and Annemiek Schilder. 2010. Anthracnose fruit rot resistance in blueberries: Evaluation of a rapid screening technique and correlation with fruit characteristics. Abstract/Poster: American Phytopathological Society, Portland, OR. Phytopathology 100:S83. Avila, Laura, Miles, Timothy, Kirk, William, and Annemiek Schilder. 2010. Screening for strobilurin (QoI) resistance in grape powdery mildew populations in Michigan. Abstract/Poster: American Phytopathological Society, Portland, OR. Phytopathology 100:S9. Miles, Timothy and Annemiek Schilder. 2009. First report of grape root rot caused by Roesleria subterranea in Michigan. Poster: Great Lakes Fruit, Vegetable and Farm Market Expo Poster Session. Grand Rapids, MI. Miles, Timothy and Annemiek Schilder. 2009. Appressorium formation and growth of Colletotrichum acutatum at different temperatures and stages of blueberry fruit development. Abstract/Oral: American Phytopathological Society, Portland, OR. Phytopathology 99:S86. Miles, Timothy and Annemiek Schilder. 2009. Evaluation of different inoculation techniques for resistance screening of blueberry fruit to anthracnose fruit rot. Abstract/Poster: American Phytopathological Society, Portland, OR. Phytopathology 99:S86. Miles, Timothy and Annemiek Schilder. 2009. First report of grape root rot caused by Roesleria subterranea in Michigan. Abstract/Poster: American Phytopathological Society, Portland, OR. Phytopathology 99:S86. Miles, Timothy and Annemiek Schilder. 2009. Investigating the molecular mechanisms of resistance to anthracnose fruit rot in blueberries. Abstract/Poster: Plant Science Graduate Students Research Symposium, East Lansing, MI. Miles, Timothy, Sysak, Roger and Annemiek Schilder. 2008. Can we slow black root rot progression and associated decline in strawberry plantings? Great Lakes Fruit, Vegetable and Farm Market Expo Poster Session. Grand Rapids, MI. 2
Miles, Timothy and Annemiek Schilder. 2008. Investigating the molecular mechanisms of resistance to anthracnose fruit rot in blueberries. Abstract/Poster: American Phytopathological Society, Minneapolis, MN. Phytopathology 98:S106 Miles, Timothy and Annemiek Schilder. 2008. Comparing scouting methods for mummy berry in highbush blueberry fields. Abstract/Poster: Plant Science Graduate Students Research Symposium, East Lansing, MI. Timothy Miles. 2007. Exploring the mechanism of resistance to Colletotrichum acutatum in blueberries. Departmental Research Seminar. Department of Plant Pathology, Michigan State University, East Lansing, MI. Miles, Timothy and Annemiek Schilder. 2007. Comparing scouting methods for mummy berry in highbush blueberry fields. Great Lakes Fruit, Vegetable and Farm Market Expo Poster Session. Grand Rapids, MI. Tefft, Nicholas, Miles, Timothy, and Wendy Ransom-Hodgkins. 2007. Functional analysis of the eukaryote elongation factor one alpha gene family. Abstract/Poster: Botany and Plant Biology Joint Congress, Chicago, IL. Miles, Timothy, Korchnak, Greg, and Wendy Ransom-Hodgkins. 2006. Loss-offunction in the eukaryotic elongation factor one alpha gene family differentially effects development. Abstract/Poster: Midwest American Society of Plant Biology, Chicago, IL. Timothy Miles. 2005. The study of eef1α in the model organism Arabidopsis thaliana. Undergraduate Research Seminar, Department of Biological Sciences, Western Michigan University, Kalamazoo, MI. Extension Publications and Presentations: Miles, Timothy and Annemiek Schilder. 2010. Disease management. 16 of 18 issues. Michigan Blueberry IPM Newsletter. http://www.isaacslab.ent.msu.edu/blueberryscout/ Miles, Timothy, and Annemiek Schilder. 2010. Keep scouting blueberries for disease symptoms. Fruit Crop Advisory Team Newsletter, May 11. Miles, Timothy, and Annemiek Schilder. 2010. Mummy berry poised for action in blueberries. Fruit Crop Advisory Team Newsletter, April 13. Miles, Timothy and Annemiek Schilder. 2009. Disease management. 18 of 21 issues. Michigan Blueberry IPM Newsletter. http://www.isaacslab.ent.msu.edu/blueberryscout/ Miles, Timothy, and Annemiek Schilder. 2009. Overview of blueberry diseases during the 2009 season. Fruit Crop Advisory Team Newsletter, September 22. Miles, Timothy, and Annemiek Schilder. 2009. If blueberries are still in bloom, keep up mummy berry sprays. Fruit Crop Advisory Team Newsletter, June 2. Timothy Miles. 2009. Grower Meeting: Mummy berry disease update and new fungicide registration of Omega 500F for highbush blueberries. Grand Junction, MI. May 13. Miles, Timothy, and Annemiek Schilder. 2009. Time to scout for mummy berry in blueberry fields. Fruit Crop Advisory Team Newsletter, April 21. 3
Schilder, Annemiek and Timothy Miles. 2008. Virus and Viruslike diseases of Blueberries. In: Michigan Blueberry Facts bulletin series. Michigan State University Extension Bulletin (E-3048) Schilder, Annemiek, Wharton, Phillip, and Timothy Miles. 2008. Mummy Berry. In: Michigan Blueberry Facts bulletin series. Michigan State University Extension Bulletin (E-2846) Miles, Timothy, and Annemiek Schilder. 2008. Anthracnose Fruit Rot (Ripe Rot). In: Michigan Blueberry Facts bulletin series. Michigan State University Extension Bulletin (E-3039) Isaacs, Rufus, Miles, Timothy, and Annemiek Schilder. 2008. Blueberry Aphid and Shoestring Virus. In: Michigan Blueberry Facts bulletin series. Michigan State University Extension Bulletin (E-3050) Timothy Miles. 2008. Presentation at grower meeting: Scouting demonstration for diseases in highbush blueberry. West Olive, MI. July Timothy Miles. 2008. Presentation at grower meeting: Possible management strategies for strawberry black root rot. Hudsonville, MI. June Miles, Timothy and Annemiek Schilder. 2008. Disease update. 15 of 15 issues. Miles, Timothy and Annemiek Schilder. 2008. Pest of the week. 7 of 15 issues. Miles, Timothy, and Annemiek Schilder. 2008. Mummy berry shoot strikes sighted in blueberries. Fruit Crop Advisory Team Newsletter, May 13. Timothy Miles. 2007. Grower Meeting: Scouting demonstration for diseases in highbush blueberry. West Olive, MI and Grand Junction, MI. July. Miles, Timothy and Annemiek Schilder. 2007. Disease update. 16 of 16 issues. Miles, Timothy and Annemiek Schilder. 2007. Pest of the week. 7 of 16 issues Schilder, Annemiek and Timothy Miles. 2007. Mummy berry update in blueberries. Fruit Crop Advisory Team Newsletter, May 15. Teaching and Activities: Served as Treasurer and Secretary for the Plant Pathology Club, Michigan State University from 2009-2010. Served as President for the Plant Pathology Club, Michigan State University from 2008-2009. Served as Secretary for the Plant Pathology Club, Michigan State University from 2007-2008. 4
Awards: Teaching Assistant, Introduction to Plant Pathology (PLP405) Michigan State University Spring 2008 Fruit Industry Scholarship recipient. Awarded December 2009. 1,000 dollars. Michigan State University A.L. Rogers Endowed Research scholarship recipient. Awarded June 2008. 2,000 dollars Professional Affiliations: American Phytopathological Society (APS) American Society of Plant Biologists (ASPB) Research Proposals aided with: Etiology and management of new and emerging blueberry diseases Michigan State University GREEEN 2011. Investigator: Schilder (pending) Inheritance and mapping of resistance to anthracnose fruit rot in highbush blueberries for marker-assisted selection Michigan State University GREEEN 2011. Investigator: Schilder (pending) In-season survival of Colletotrichum acutatum in blueberries. Michigan State University, GREEEN 2009. Investigator: Schilder. (not funded) Developing a virus-induced gene silencing (VIGS) protocol for blueberries. Michigan State University, GREEEN 2008. Investigator: Schilder. (not funded) The molecular bases for different infection strategies of Colletotrichum acutatum and host resistance in blueberries. USDA-NRI 2007. Investigators: Schilder, Day, and Nair. (not funded) Meetings attended 9 th International Vaccinium Meeting (2008) Great Lakes Expo 2006, 2007, 2008, 2009, and 2010 Annual APS Meeting 2009 and 2010 North American Blueberry Research and Extension Workers Conference (2010) 5