LIST OF RECENT PLANTSCREEN TM INSTALLATIONS The below-listed overview is example of recent PlantScreen TM high-throughput automated Systems manufactured and installed by the Photon Systems Instruments, spol. s.r.o. (PSI). PlantScreen TM Conveyor System for Rice and Arabidopsis plants DGIST, Daegu, South Korea (Prof. Hong Gil Nam) Description: Phenotyping PlantScreen TM conveyor system for high-throughput phenotyping of arabidopsis plants and rice plants with size up to 1.4 meters throughout the full life-span. Two growth chambers customized for arabidopsis and rice plants. Location: DGIST & IBS, Nam Laboratory for Complex Biology, Daegu Kyeongbuk Institute of Science & Technology (DGIST), 50-1 Sang-Ri, Hyeonpung-Myeon, Dalseong-Gun, Daegu 711-873, South Korea Installed: 2014 Layout Schematics: Rice Room Phenotyping Unit Arabidopsis Room Schematic layout of the Daegu installation. Configuration: 1. PlantScreen Conveyor System: The customized phenotyping system consists of two walk-in FytoScope growth chambers designed for controlled growth of arabidopsis and rice plants. Temperature, humidity and CO 2 are controlled and/or monitored. A single central Phenotyping Unit integrates a number of imaging stations for automated plant phenotyping including chlorophyll fluorescence imaging, RGB morphometric imaging, thermal imaging, VNIR and SWIR hyperspectral imaging unit and a unique 3D imaging unit. Plants are automatically transported on conveyors to the central phenotyping unit. The capacity is up to 2800 arabidopsis plants in standardized pots in the arabidopsis room, and 210 rice plants in customized pots in the rice room. An integral part of the Phenotyping Unit is an acclimation tunnel for light and dark adaptation of the plants prior measurement with cool white, red and FAR LEDs with light intensity up to 1000 µmol/m 2 /s. A weighing 1
and watering station is inluded. A comprehensive software package allowing for remote accessibility and control is provided for all aspects of the conveyor and imaging modules, as well as for data acquisition, image analysis and data base configuration. Sensors RGB and Morphometric Imaging Kinetic Chlorophyll Fluorescence Imaging VNIR Hyperspectral Imaging SWIR Hyperspectral Imaging 3D Imaging Thermal Imaging Environmental Sensors Applications Shoot biomass, growth dynamics, shoot shape, color index etc. Photosynthetic status, quantum yield, nonphotochemical quenching, ETR, GFP, anthocyanin content etc. Pigment composition, biochemical compounds, nitrogen content etc. Biochemical compounds, leaf water status etc. Shoot structure, leaf angle distribution, shoot biomass etc. Surface temperature Temperature, light intensity, CO2, humidity etc. Phenotyping Unit 2
2. Walk-In FytoScope Chambers: The growing lines for Arabidopsis plants (7 lines, each line for 20 trays, up to 2800 plants) and for rice plants (7 lines, each line for 15 trays, up to 210 plants), are installed in walk-in FytoScope growth chambers. The walk-in chambers utilize Light Emitting Diodes (LED's) as the sole light source and thus provide excellent spectral quality with high irradiance. The FytoScope chambers allow for independent selection of photoperiods. Environmental conditions as temperature, relative humidity, lightning intensity and regime, light spectral quality, irradiation cycles and CO2 concentration are controlled via FytoScope control software, which can be accessed remotely. Actual conditions and target values for temperature, lighting and relative humidity are permanently displayed on the touch-screen controller, which is situated on the front side of the FytoScope. The controller allows a wide range of user-programmable options to be selected. Graphical representations of actual conditions inside the FytoScope are displayed. All data can be downloaded to a PC. Arabidopsis and Rice Walk-In FytoScope Chamber 3
PlantScreen Conveyor System IBERS, Aberystwyth, United Kingdom (Prof. John Doonan) Description: PlantScreen TM Conveyor System for high-throughput phenotyping of arabidopsis plants and plants with height up to 60 cm. Location: Institute of Biological, Environmental & Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion SY23 3EE, Wales, UK. Installed: 2015 Layout Schematics: Configuration: The phenotyping unit is located inside a glasshouse and contains 5 growing lines with capacity for 2000 plants. Each growing line contains 20 trays for Arabidopsis, small crop and other cereal plants in standardized pots. The system 4
includes an adaptation tunnel with LED illumination (cool white and FAR LEDs up to 1000 µmol/m 2 /s) for light and dark adaptation of plants prior to measurement. Two measuring cabinets are included and a weighing and watering station. One measuring cabinet contains a chlorophyll fluorescence imaging unit, and the second is used for RGB and morphometric analysis. The RGB/morphometric unit has a turntable for multi-angle side view RGB imaging. A comprehensive software package allowing for remote accessibility and control is provided for all aspects of the conveyor and imaging modules, as well as for data acquisition, image analysis and data base configuration. Sensors RGB and Morphometric Imaging Kinetic chlorophyll fluorescence Imaging Environmental Sensors Applications Shoot biomass, growth dynamics, shoot shape, color index etc. Photosynthetic status, quantum yield, nonphotochemical quenching, ETR etc. Temperature, light intensity, CO2, humidity etc. 5
PlantScreen TM Conveyer System and PlantScreen TM Robotics XYZ System in Controlled Environments Center of Excellence Hana, Czech Republic (Dr. Lukas Spichal) Description: High-throughput plant phenotyping facility located in controlled climate chambers with LED illumination. Location: Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 11, Olomouc, CZ-78371 Czech Republic. Installed: 2013 Layout Schematics: 1. PlantScreen TM Conveyer System: 2. PlantScreen TM Robotics XYZ System: 6
Configuration: 1. PlantScreenTM Conveyor System: capacity for high throughput phenotyping of up to 640 arabidopsis, small crop and other cereals plants in standardized pots. The phenotyping system is located in controlled climate chambers (Fytoscopes) with LED illumination and imaging cabinet and acclimation cabinet. The measuring cabinet incorporates imaging stations for chlorophyll fluorescence kinetics, RGB/morphometric analysis, thermal imaging and an SWIR hyperspectral imaging unit. The acclimation cabinet is designed for light and dark adaptation and contains a module for watering and weighing plants. A comprehensive software package with remote accessibility is provided for controlling all aspects of the conveyor and imaging modules, as well as for data acquisition, image analysis and data base configuration. Sensors RGB and Morphometric Imaging Kinetic chlorophyll fluorescence Imaging SWIR hypespectral Imaging Thermal Imaging Environmental Sensors Applications Shoot biomass, growth dynamics, shoot shape, color index, Photosynthetic status, quantum yield, nonphotochemical quenching, ETR,.. Biochemical compounds, leaf water status, Surface temperature Temperature, Rh, light intesity, light quality 2. PlantScreenTM Robotics XYZ System: capacity for high throughput phenotyping of up to 1200 arabidopsis plants in standardized pots or 480 multiwell plates in standardized positions. The system incorporates a number of instruments for imaging of plant morphometric and physiological parameters on the robotic XYZ arm. The XYZ robotic arm carries 7
sensors for chlorophyll fluorescence imaging, RGB/morphometric imaging and VNIR hyperspectral imaging directly to the plants. Plant selection for imaging is pre-defined in soiftware. A comprehensive software package with remote accessibility is provided for controlling all aspects of the conveyor and imaging modules, as well as for data acquisition, image analysis and data base configuration. Sensors RGB and Morphometric Imaging Kinetic chlorophyll fluorescence Imaging VNIR hypespectral Imaging Environmental Sensors Applications Shoot biomass, growth dynamics, shoot shape, color index, Photosynthetic status, quantum yield, nonphotochemical quenching, ETR,.. Pigment composition, biochemical compounds, nitrogen content, Temperature, Rh, light intesity, light quality 3. FytoScope chambers: Both plant phenotyping facilities are installed inside FytoScope controlled environment chambers with LED illumination. The FytoScope chamber regulate temperature (+10 to +40 C), irradiation cycles (with PAR irradiance up to 1500 µe) and relative humidity (40% to 90%). Multifaceted programming options are used to simulate natural conditions and to define a full range of "day/night" cycles with "dawn/dusk" or "cloudy sky" effects. Actual inside conditions and target values for temperature, lighting and relative humidity are permanently displayed on 8
the touch-screen controller. The controller allows a wide range of user programmable options to be selected. All data may be downloaded to a PC and accessed remotely. References: Humplik J.F., Furst T., Husickova A., Hybl M., Spichal L. Automated phenotyping of plant shoots using imaging methods for analysis of plant stress responses a review. Plant Methods 2015; 11: 29. Humplik J.F., Lazar D., Furst T., Husickova A., Hybl M., Spichal L. Automated integrative high-throughput phenotyping of plant shoots: a case study of the cold-tolerance of pea (Pisum sativum L.) Plant Methods 2015; 11: 20. 2015/06 PSI (Photon Systems Instruments),spol. s.r.o. 9