LABORATORY WASTE MANAGEMENT GUIDELINES

LABORATORY WASTE MANAGEMENT GUIDELINES Environmental Health & Safety

LABORATORY WASTE MANAGEMENT GUIDELINES The following document comprises the Laboratory Waste Management Guidelines for the University of Texas at Tyler. These guidelines are distributed to the following departments that generate or have the potential to generate laboratory waste on campus: Art Biology Chemistry Electrical Engineering Mechanical Engineering Health & Kinesiology Psychology Physical Plant The plan is reviewed annually and updated as necessary. Initial Plan developed May 1999 Latest Update: May, 2003 2

Table of Contents I. INTRODUCTION... 4 II. HAZARDOUS WASTE... 4 A. CHARACTERISTIC D WASTE... 4 1. Ignitability D001... 4 2. Corrosivity D002... 5 3. Reactivity D003... 5 4. Toxicity D004-D043... 5 B. LISTED WASTE... 5 1. F List... 6 2. K List... 6 3. P and U Lists... 6 III. SPECIAL WASTE... 6 IV. WASTE MANAGEMENT GUIDELINES... 7 V. DISPOSAL PROCEDURES... 8 A. CHEMICAL WASTE... 8 1. Procedure... 8 2. Disposal... 8 B. BIOLOGICAL WASTE... 9 1. Procedure... 9 2. Collection And Disposal... 9 VI. POLLUTION PREVENTION... 9 VII. RCRA HAZARDOUS WASTE LISTS... 11 A. TOXICITY CHARACTERISTIC WASTE D004 D0043... 11 B. F WASTES NON-SPECIFIC SOURCE WASTES... 12 C. ACUTE TOXIC HAZARDOUS WASTE P LIST... 13 D. TOXIC HAZARDOUS WASTE- U LIST... 13 VIII. CITY OF TYLER DRAIN DISCHARGE PROHIBITIONS... 16 A. GENERAL PROHIBITIONS... 16 B. SPECIFIC PROHIBITIONS... 16 IX. FORMS... 17 A. CHEMICAL WASTE ACCUMULATION LOG... 18 B. SAMPLE CHEMICAL WASTE ACCUMULATION LOG... 19 C. CHEMICAL WASTE LABEL... 20 D. SAMPLE CHEMICAL WASTE FORM... 20 E. BIOWASTE COLLECTION FORM... 21 3

I. Introduction Laboratory wastes, whether chemical or biological, can pose a significant risk to human health and the environment if not handled correctly. The University of Texas at Tyler is committed to making sure that all wastes generated on it s campuses are identified, contained, and disposed of in accordance with applicable laws, regulations, and best management practices. The Resource Conservation and Recovery Act (RCRA) of 1976 and subsequent amendments were passed by Congress to provide the legislative basis for hazardous waste management in the United States. The Environmental Protection Agency (EPA) is the federal agency responsible for the administration and enforcement of RCRA. In Texas, the EPA has delegated authority for administrating and enforcing RCRA regulations to the Texas Council on Environmental Quality (TCEQ). EPA and TCEQ hazardous waste regulations are designed to provide for the protection of public health and the environment through stringent requirements for generators, transporters, and processors of hazardous waste. While RCRA s major focus is on the management of hazardous waste from the point of generation to the point of disposal, referred to as cradle to grave, it also covers solid waste management in general and encourages recycling and alternative energy sources. II. Hazardous Waste A solid waste is any solid, liquid, semi-solid or contained gaseous material that is discarded, abandoned, recycled, or is an inherently waste-like material... (20 CFR 261.2). A regulated chemical waste is defined as a waste which, due to its quantity, concentration, or physical and chemical characteristics may cause, or significantly contribute to, an increase in mortality or an increase in serious or incapacitating illness; or pose a substantial present or potential threat to human health or the environment when improperly treated, stored, transported, disposed of, or otherwise managed. RCRA defines a hazardous waste as a sold waste that is either characteristic or listed. A. Characteristic D Waste The EPA has identified four characteristics for hazardous waste: Ignitability, Corrosivity, Reactivity and Toxicity. Any solid waste exhibiting any one or more of these characteristics is hazardous and subject to RCRA regulation. 1. Ignitability D001 a. liquids having a flash point below 140 F b. solids that, under STP, may cause fire through friction, absorption of moisture, or spontaneous chemical changes c. certain ignitable compressed gases 4

d. oxidizers 2. Corrosivity D002 Any aqueous solution with a ph of less than or equal to 2 or greater than or equal to 12.5 or any liquid that will corrode steel faster than 1/4 inch per year. 3. Reactivity D003 Any wastes exhibiting one or more of the following characteristics are classified as reactive wastes and require specially storage and handling during transportation to the treatment facility. Unstable materials capable of undergoing violent chemical change (without detonating). Materials which react violently with water. Materials which form potentially explosive mixtures with water. Materials which, when mixed with water, generate toxic gases, vapors, or fumes in a quantity sufficient to present a danger to human health or the environment. Cyanide or sulfide bearing wastes which, when exposed to ph conditions between 2 and 12.5, will generate toxic gases, vapors, or fumes in a quantity sufficient to present a danger to human health or the environment. Materials capable of detonation or explosive reaction when subjected to a strong initiating source or if heated in confinement. Materials which are capable of detonation or explosive decomposition at standard temperature and pressure. Alkali metals, peroxides, and cyanide and sulfide compounds are examples of compounds that become reactive wastes. 4. Toxicity D004-D043 A solid waste whose leachate (extract) contains any of the toxic heavy metals, pesticides, or organics in concentrations equal to or greater than the regulatory levels given in the table Toxicity Characteristic Waste D004 D0043. B. Listed Waste The EPA has also designated hazardous waste to be any solid waste that is listed in one of four following lists: 5

1. F List F listed hazardous wastes are generic wastes from non-specific sources. Wastes on this list include spent halogenated and non-halogenated solvents as shown in table F Wastes Non-Specific Source Wastes. The F001 waste code is used ONLY for degreasing wastes. 2. K List K listed hazardous waste are specific wastes from specifically identified industries (sources). This does not apply to UTT. 3. P and U Lists P and U listed hazardous wastes are discarded commercial chemicals, offspecification chemicals, and container or spill residues from such compounds, including unused laboratory chemicals. P listed wastes (Acute Toxic Hazardous Waste P List) are acute hazardous waste, and no more than 1 kg of such waste may be generated in any one month, while U listed wastes (Toxic Hazardous Waste- U List) are toxic waste. Note: If the waste is spent (not in its original form), these lists do not apply. III. Special Waste Many laboratory chemicals used at UT Tyler are known to be toxic or otherwise hazardous but are not characteristic or listed wastes and therefore not RCRA hazardous wastes. Examples include ethidium bromide. The TCEQ recognizes this and has developed a waste category for these special wastes. A special waste is any solid waste that is not regulated as hazardous but because of its quantity, concentration, physical and/or chemical characteristics, or biological properties requires special handling and disposal to protect human health and the environment. The following are considered special waste: 1. Biological/medical (biomedical) waste including research animal tissues or carcasses, cultures, and sharps (razor blade, syringe needles) 2. Waste whose toxic constituent concentration falls below the regulatory limits for the toxicity characteristic 3. Petroleum products, including oil and oil filters 4. Empty hazardous chemical containers 5. Light ballasts and small capacitors containing PCBs 6. Asbestos (friable) 7. Lead acid and Nickel/Cadmium (NiCad) batteries 6

IV. Waste Management Guidelines These guidelines are very important to avoid unnecessary handling and/or costs and to comply with federal and state regulations. 1. Never discard hazardous or special waste down the drain ( City Of Tyler Drain Discharge Prohibitions) or into the trash. These wastes must be collected for proper disposal. 2. Establish a Satellite Accumulation (SA) area in each laboratory where wastes will be generated. EH&S will help determine the best location for your SA and will provide appropriate labeling as well as emergency telephone number labels. 3. Label each container (Chemical Waste Label) and place in your SA area. Make sure each container has a tight-fitting lid. Principal component (excluding water) should be listed on label if it is a mixture. Labels templates are available from EH&S for small (Avery Label #5294) and large (Avery Label #5164) waste labels. 4. All containers must be clearly identified and labeled with the proper chemical name(s) of the substance(s) at the start of collection. Trade names, acronyms, abbreviations, codes, or formulas are not acceptable. 5. Do not accumulate waste in containers greater than five gallons and do not fill the waste containers entirely; leave a adequate space (such as the entire neck of the bottle) at the top so the contents of the container can be transferred without spillage. 6. Do not mix organic and aqueous waste together in the same waste container. Water decreases the BTU value of flammable organic solvents and therefore reduces its usability as a fuel. 7. Do not mix halogenated organic solvent waste with non-halogenated organic solvent waste. Most halogenated organic solvents are persistent environmental hazards and expensive to dispose of and therefore should be kept separate from other wastes. 8. Evaporation of solvents, especially chlorofluorocarbons (CFCs), for the purpose of disposal is not permitted. 9. Inexperienced laboratory personnel should not perform neutralization of acid and bases waste; instead, contact EH&S for disposal. Bulk neutralization of acid and base waste is prohibited. If experienced personnel perform neutralizations of trace amounts of acid and base waste in the lab, be certain that there are not other hazardous constituents in the wastes and that neutralization is complete and within the City of Tyler drain disposal limits (page 16) before disposing down the drain. Such solutions must have a ph between 5.5 and 10.5 be relatively non-toxic, and not exceed specific metal limits. 10. Some empty containers of chemicals can be disposed of in the ordinary trash. At UT Tyler a chemical container is considered empty if all material has been removed and the container is tripled rinsed with appropriate solvent and the rinsate being handle as hazardous waste. If uncertain about what the appropriate solvent is to use with a particular chemical, or how to triple rinse to minimize generation of hazardous waste, please contact EH&S for assistance. 11. Do not discard into the trash empty chemical containers that contained any of the following: acids, bases, solvents, or any P or U listed chemicals. These 7

containers must be rendered unusable before disposal. All empty containers of chemicals must be triple rinsed with an appropriate solvent prior to disposal. The waste solvent must be disposed of as hazardous waste. An alternative is to dispose of the container as hazardous waste. University faculty and staff should contact EH&S at 7011 for guidance on how to prepare these containers for disposal. 12. Spent lubricating oils must be collected for recycling purposes; therefore, do not contaminate with any other wastes. 13. Prevent unknowns by keeping all waste containers labeled. The burden of identifying unknowns lies with the laboratory generating the unknown and not EH&S. Wastes will generally not be accepted if the contents are not known. V. Disposal Procedures Environmental Health & Safety (EH&S) provides chemical and special waste pickup and disposal services for U Tyler. These services are provided at no cost to the requesting department; however the following guidelines must be followed to ensure compliance with federal and state laws and to avoid unnecessary handling and/or costs. A. Chemical Waste 1. Procedure a. Fill out an accumulation log (page 18) using the same waste log number as on the label. This log sheet is required for each waste container. b. The Chemical name (spelled out, no abbreviations or structures), CAS#, solvent (if applicable), must be listed as each waste is added to the container. Identify all chemicals in the waste, including water (unknowns are not acceptable) When container is full, add percentage of each component present. c. Make sure that all wastes are chemically compatible before placing in the container. d. Use only chemically compatible containers and label (page 20) properly. e. Wastes must be segregated according to the type of hazard present. f. Use a waste label to identify the waste, the location, the log sheet number, and the responsible person. The generator is responsible for the identification of the waste. However, if the generator cannot positively identify the waste, the waste will be sent to a contract laboratory for analysis and the generator will be billed for the analytical costs. g. Complete the Chemical and Physical Hazard section. 2. Disposal a. Unless actively receiving wastes, container should be stored in the SA area with the cap securely in place. b. When the waste container is full call the EH&S office at 566-7011 and tape a copy of this waste log to the container. 8

B. Biological Waste Bio waste - any solid waste or a combination of solid wastes that because of its quantity or biological properties requires special handling and disposal to protect human health and the environment. Bio waste includes, but is not limited to: infectious and pathological materials from health care facilities, veterinary hospitals or laboratories; such wastes are those containing pathogens or biologically active material that, because of its type, concentration and quantity, is capable of transmitting disease, and any animal tissues or carcasses in need of disposal. 1. Procedure a. Appropriately marked and labeled containers and boxes with liners will be stored for accessible use at collection sites. Collection sites include designated classrooms and laboratories. b. Collection of waste will be coordinated by EH&S. Collection boxes, liners, sharps containers, and red bags are available from EH&S. c. Animal body parts that have been intentionally exposed to pathogens are treated to kill infectious organisms and placed in sealed containers. d. Needles and glass are placed in appropriate sharps containers and sealed. e. All other biomedical waste are placed directly in appropriate biomedical waste containers and sealed. f. All animal carcasses used in laboratories for teaching/research purposes and need disposal. g. Personal protective equipment will be worn when handling biomedical waste. When biomedical waste containers are filled to the maximum level at the site of collection, they are properly sealed and stored pending pick up by EH&S. 2. Collection And Disposal a. When biomedical waste is ready for pick up from a collection site, the respective department will complete the Biowaste Collection Form (page 21). b. Call EH&S at 7011 to arrange for waste pickup. EH&S will pick up the waste and store it in a central location. c. All waste will be picked up from the approved collection sites. Do not place waste in corridors. d. Labeled waste is picked up from a central storage location by an approved contractor, as requested by EH&S. The contractor transports the waste to an approved waste management company for incineration. Records of incineration and completed waste collection forms are kept on file in the EH&S office. VI. Pollution Prevention The following are some source reduction and waste minimization guidelines to assist you in reducing the hazardous waste that your lab generates. 9

1. Minimize mixing hazardous waste with non-hazardous waste, such as water. Do not dilute hazardous waste. This not only increases the volume necessary for disposal, but may also affect any reusable properties of the waste, such as BTU value. The only exception is adding water to explosive chemicals to keep them wet. 2. Segregate your waste according to waste streams such as: non-halogenated organic solvent waste (no water); halogenated organic solvent waste (no water); photo fixer waste; aqueous waste with non-halogenated organic solvents; aqueous waste with halogenated organic solvents; aqueous waste with toxic heavy metals; aqueous acidic waste; aqueous basic waste; metallic mercury waste; lubricating oil; formalin; and ethidium bromide, etc. 3. Avoid contamination of stock chemicals. Unused portions of a chemical should not be returned to its original container. Estimate how much of a chemical will be needed for an experiment to avoid removing more chemical than is needed. For teaching labs, preweigh chemicals for undergraduate usage. 4. Substitute less hazardous chemicals in experiments whenever possible. 5. Use spirit-filled thermometers instead of mercury-filled thermometers. 6. If only a small amount of a chemical is needed, ask around other labs before ordering. 7. Avoid buying chemicals in bulk. Cost of disposal usually outweighs any savings obtained by buying in bulk. 10

VII. RCRA Hazardous Waste Lists A. Toxicity Characteristic Waste D004 D0043 EPA Waste No. Constituent CAS Number Regulatory Level (mg/l) D004 Arsenic 7440-38-2 5.0 D005 Barium 7440-39-3 100.0 D018 Benzene 71-43-2 0.5 D006 Cadmium 7440-43-9 1.0 D019 Carbon Tetrachloride 56-23-5 0.5 D020 Chlordane 57-74-9.03 D021 Chlorobenzene 108-90-7 100.0 D022 Chloroform 67-66-3 6.0 D007 Chromium 7440-47-3 5.0 D026 Cresol ----------- 200.0 D016 2,4-D 94-75-7 10.0 D027 1,4-Dichlorobenzene 106-46-7 7.5 D028 1,2-Dichloroethane 107-06-2 0.5 D029 1,1-Dichloroethylene 75-35-4 0.7 D030 2,4-Dinitrotoluene 121-14-2 0.13 D012 Endrin 72-20-8 0.02 D031 Heptachlor (and its hydroxide) 76-44-8 0.008 D033 Hexachloro-1,3-butadiene 87-68-3 0.5 D032 Hexachlorobenzene 118-74-1 0.13 D034 Hexachloroethane 67-72-1 3.0 D008 Lead 7439-92-1 5.0 D013 Lindane 58-89-9 0.4 D024 m-cresol 108-39-4 200.0 D009 Mercury 7439-97-6 0.2 D014 Methoxychlor 72-43-5 10.0 D035 Methyl ethyl ketone 78-93-3 200.0 D036 Nitrobenzene 98-95-3 2.0 D023 o-cresol 95-48-7 200.0 D025 p-cresol 106-44-5 200.0 D037 Pentachlorophenol 87-86-5 100.0 D038 Pyridine 100-86-1 5.0 D010 Selenium 7782-49-2 1.0 D011 Silver 7440-22-4 5.0 D039 Tetrachloroethylene 127-18-4 0.7 D015 Toxaphene 8000-35-2 0.5 D017 2,4,5-TP (Silvex) 93-72-1 1.0 D040 Trichloroethylene 79-01-6 0.5 D041 2,4,5-Trichlorophenol 95-95-4 400.0 D042 2,4,6-Trichlorophenol 88-06-2 2.0 D043 Vinyl chloride 75-01-4 0.2 11

B. F Wastes Non-Specific Source Wastes [Note: If the waste is not spent (in its original form) this list does not apply.] EPA # F001 F002 F003 F004 F005 Nonspecific Source Waste Description The following spent halogenated solvents used in degreasing: tetrachloroethylene, trichloroethylene, methylene chloride, 1,1,1-trichloroethane, carbon tetrachloride, and chlorinated fluorocarbons; all spent solvent mixtures/blends used in degreasing containing, before use, a total of ten percent or more (by volume) of one or more of the above halogenated solvents or those solvents listed in F002, F004, and F005; and still bottoms from the recovery of these spent solvents and spent solvent mixtures The following spent halogenated solvents: tetrachloroethylene, methylene chloride, trichloroethylene, 1,1,1-trichloroethane, chlorobenzene, 1,1,2-trichloro-1,2,2- trifluoroethane, ortho- dichlorobenzene, trichlorofluoromethane, and 1,1,2- trichloroethane; all spent solvent mixtures/blends containing, before use, a total of ten percent or more (by volume) of one or more of the above halogenated solvents or those listed in F001, F004, or F005; and still bottoms from the recovery of these spent solvents and spent solvent mixtures The following spent non-halogenated solvents: xylene, acetone, ethyl acetate, ethyl benzene, ethyl ether, methyl isobutyl ketone, n-butyl alcohol, cyclohexanone, and methanol; all spent solvent mixtures/blends containing, before use, only the above spent non-halogenated solvents; and all spent solvent mixtures/blends containing, before use, one or more of the above non-halogenated solvents, and, a total of ten percent or more (by volume) of one or more of those solvents listed in F001, F002, F004, and F005; and still bottoms from the recovery of these spent solvents and spent solvent mixtures The following spent non-halogenated solvents: cresols and cresylic acid, and nitrobenzene; all spent solvent mixtures/blends containing, before use, a total of ten percent or more (by volume) of one or more of the above non-halogenated solvents or those solvents listed in F001, F002, and F005; and still bottoms from the recovery of these spent solvents and spent solvent mixtures The following spent non-halogenated solvents: toluene, methyl ethyl ketone, carbon disulfide, isobutanol, pyridine, benzene, 2-ethoxyethanol, and 2-nitropropane; all spent solvent mixtures/blends containing, before use, a total of ten percent or more (by volume) of one or more of the above non-halogenated solvents or those solvents listed in F001, F002, or F004; and still bottoms from the recovery of these spent solvents and spent solvent mixtures 12

C. Acute Toxic Hazardous Waste P List Chemical Name EPA # Chemical Name EPA # 1-Acetyl-2-thiourea P002 Fluoroacetic acid, sodium salt P058 Acrolein P003 Heptachlor P059 Aldicarb P070 Hexaethyl tetraphosphate P062 Aldrin P004 Isodrin P060 Allyl alcohol P005 Mercury fulminate P065 Aluminium phosphide P006 Methomyl P066 5-(Aminomethyl)-3-isoxazolol P007 Methyl hydrazine P068 4-Aminopyridine P008 Methyl isocyanate P064 Ammonium picrate P009 2-Methyllactonitrile P069 Ammonium vanadate P119 Methyl parathion P071 Arsenic acid P010 α-naphthylthiourea P072 Arsenic pentoxide P011 Nickel carbonyl P073 Arsenic trioxide P012 Nicotine, and salts P075 Benzenethiol P014 Nitric oxide P076 Benzyl chloride P028 p-nitroaniline P077 Beryllium powder P015 Nitrogen dioxide P078 Bromoacetone P017 Nitroglycerine P081 Brucine P018 N-Nitrosodimethylamine P082 Carbon disulfide P022 N-Nitrosomethylvinylamine P084 Chloroacetaldehyde P023 Octamethylpyrophosphoramide P085 p-chloroaniline P024 Osmium tetroxide P087 l-(o-chlorophenyl)thiourea P026 Parathion P089 3-Chloropropionitrile P027 Phenylmercury acetate P092 Cyanides (soluble salts) P030 Phentlthiourea P093 Cyanogen P031 Phorate P094 Cyanogen chloride P033 Phosgene P095 2-Cyclohexyl-4,6-dinitrophenol P034 Phosphine P096 Dichloromethyl ether P016 Propanenitrile P101 Dichlorophenylarsine P036 Propargyl alcohol P102 Dieldrin P037 1,2-Propylenimine P067 Diethylarsine P038 Selenourea P103 Diethyl p-nitrophenyl phosphate P041 Sodium azide P105 Diethyl o-pyrazinyl phosphorothioate P040 Strontium sulfide P107 Diisopropylfluorophosphate P043 Strychnine, and salts P108 Dimethoate P044 Tetraethyldithiopyrophosphate P109 α,α-dimethylphenethylamine P046 Tetraethyl lead P110 4,6-Dinitro-o-cresol, and salts P047 Tetraethylpyrophosphate P111 2,4-Dinitrophenol P048 Tetranitromethane P112 Dinoseb P020 Thallium oxide P113 Disulfoton P039 Thallium(I) selenite P114 Dithiobiuret P049 Thallium(I) sulfate P115 Endosulfan P050 Thiofanox P045 Endothall P088 Thiosemicarbazide P116 Endrin P051 Toxaphene P123 Epinephrine P042 Trichloromethanethiol P118 Ethyleneimine P054 Vanadium pentoxide P120 Famphur P097 Warfarin, and salts P001 Fluorine P056 Zinc phosphide (>10%) P122 Fluoroacetamide P057 13

D. Toxic Hazardous Waste- U List Chemical Name EPA # Chemical Name EPA # Acetaldehyde U001 Cyclohexanone U057 Acetone U002 Cyclophosphamide U058 Acetonitrile U003 Daunomycin U059 Acetophenone U004 DDD U060 2-Acetylaminofluorene U005 DDT U061 Acetyl chloride U006 Diallate U062 Acrylamide U007 Dibenz[a,h]anthracene U063 Acrylic acid U008 Dibenzo[a,i]pyrene U064 Acrylonitrile U009 1,2-Dibromo-3-chloropropane U066 Amitrole U011 1,2-Dibromoethane U067 Aniline U012 Dibromomethane U068 Auramine U014 Dibutyl phthalate U069 Azaserine U015 o-dichlorobenzene U070 Benz[c]acridine U016 m-dichlorobenzene U071 Benzal chloride U017 p-dichlorobenzene U072 Benz[a]anthracene U018 3,3 -Dichlorobenzidine U073 Benzene U019 1,4-Dichloro-2-butene U074 1,3-Benzenediol U201 Dichlorodifluoromethane U075 Benzenesulfonic acid chloride U020 1,1-Dichloroethane U076 Benzidine U021 1,2-Dichloroethane U077 Benzo[a]pyrene U022 1,1-Dichloroethylene U078 p-benzoquinone U197 1,2-Dichloroethylene U079 Benzotrichloride U023 Dichloroethyl ether U025 2,2 -Bioxirane U085 Dichloroisopropyl ether U027 Bromoform U225 Dichloromethane U080 4-Bromophenyl phenyl ether U030 Dichloromethoxy ethane U024 2-Butanone U159 2,4-Dichlorophenol U081 2-Butenal U053 2,6-Dichlorophenol U082 Butyl alcohol U031 2,4-Dichlorophenoxyacetic acid U240 Cacodylic acid U136 1,2-Dichloropropane U083 Calcium chromate U032 1,3-Dichloropropene U084 Carbonic difluoride U033 Diepoxybutane U085 Carbon tetrachloride U211 Diethylhexyl phthalate U028 Chlorambucil U035 N,N -Diethylhydrazine U086 Chlordane, α and ψ isomers U036 O,O-Diethyl S-metyl dithiophosphate U087 Chlornaphazin U026 Diethyl phthalate U088 Chlorobenzene U037 Diethylstilbestrol U089 Chlorobenzilate U038 Dihydrosafrole U090 p-chloro-m-cresol U039 3,3 -Dimethoxybenzidine U091 Chloroethene U043 Dimethylamine U092 2-Chloroethyl vinyl ether U042 p-dimethylaminoazobenzene U093 Chloroform U044 7,12-Dimethylbenz[a]anthracene U094 Chloromethyl methyl ether U046 3,3 -Dimethylbenzidine U095 2-Chloronaphthalene U047 α,α-dimethylbenzyl-hydroperoxide U096 o-chlorophenol U048 Dimethylcarbamoyl chloride U097 4-Chloro-o-toluidine, hydrochloride U049 1,1-Dimethylhydrazine U098 Chrysene U050 1,2-Dimethylhydrazine U099 Creosote U051 2,4-Dimethylphenol U101 Cresol U052 Dimethyl phthalate U102 Cumene U055 Dimethyl sulfate U103 Cyanogen bromide U246 2,4-Dinitrotoluene U105 Cyclohexane U056 2,6-Dinitrotoluene U106 13

Di n-octyl phthalate U107 1,4-Dioxane U108 1,2-Diphenylhydrazine U109 Dipropylamine U110 Di-n-propylnitrosamine U111 Epichlorohydrin U041 2-Ethoxyethanol U359 Ethyl acetate U112 Ethyl acrylate U113 Ethyl carbamate U238 Ethylenebisdithiocarbamic acid, salts & esters U114 Ethylene glycol monoethyl ether U359 Ethylene oxide U115 Ethylenethiourea U116 Ethyl ether U117 Ethyl methacrylate U118 Ethyl methanesulfonate U119 Fluoranthene U120 Formaldehyde U122 Formic acid U123 Furan U124 Furfural U125 Glycidylaldehyde U126 Hexachlorobenzene U127 Hexachlorobutadiene U128 Hexachlorocyclopentadiene U130 Hexachloroethane U131 Hexachlorophene U132 Hexachloropropene U243 Hydrazine U133 Hydrofluoric acid U134 Hydrogen sulfide U135 Indeno[1,2,3-cd]pyrene U137 Iodomethane U138 Isobutyl alcohol U140 Isosafrole U141 Kepone U142 Lasiocarpine U143 Lead acetate U144 Lead phosphate U145 Lead subacetate U146 Lindane U129 Maleic anhydride U147 Maleic hydrazide U148 Malononitrile U149 Melphalan U150 Mercury U151 Methacrylonitrile U152 Methanethiol U153 Methanol U154 MethapyrileneU155 Methoxychlor U247 Methyl bromide U029 Methyl chloride U045 Methyl chlorocarbonate U156 3-Methylchloranthrene 4,4 -Methylenebis(2-chloroaniline) Methyl ethyl ketone Methyl ethyl ketone peroxide Methyl isobutyl ketone Methyl methacrylate N-Methyl-N -nitro-n-nitrosoguanidine Methylthiouracil Mitomycin C 1-Naphtalenamine 2-Naphthalenamine Naphtalene 1,4-Naphthalenedione Nitrobenzene U169 p-nitrophenol U170 2-Nitropropane N-Nitrosodi-n-butylamine N-Nitrosodiethanolamine N-Nitrosodiethylamine N-Nitroso-N-ethylurea N-Nitroso-N-methylurea N-Nitroso-N-methylurethane N-Nitrosopiperidine N-Nitrosopyrrolidine 5-Nitro-o-toluidine Paraldehyde Pentachlorobenzene Pentachloroethane Pentachloronitrobenzene 1,3-Pentadiene Phenacetin Phenol Phosphorus sulfide Phthalic anhydride 2-Picoline Pronamide 1,3-Propane sultone n-propylamine Pyridine Reserpine Resorcinol Saccharin, and salts Safrole Selenious acid Selenium sulfide Streptozotocin 1,2,4,5-Tetrachlorobenzene 1,1,1,2-Tetrachloroethane 1,1,2,2-Tetrachloroethane Tetrachloroethylene Tetrahydrofuran Thallium(I) acetate Thallium(I) carbonate Thallium(I) chloride Thallium(I) nitrate Thioacetamide U157 U158 U159 U160 U161 U162 U163 U164 U010 U167 U168 U165 U166 U171 U172 U173 U174 U176 U177 U178 U179 U180 U181 U182 U183 U184 U185 U186 U187 U188 U189 U190 U191 U192 U193 U194 U196 U200 U201 U202 U203 U204 U205 U206 U207 U208 U209 U210 U213 U214 U215 U216 U217 U218 14

Thiourea Thiram Toluene Toluenediamine Toluene diisocyanate o-toluidine p-toluidine o-toluidine hydrochloride Trichloroacetaldehyde 1,1,1-Trichloroethane 1,1,2-Trichloroethane U219 U244 U220 U221 U223 U328 U353 U222 U034 U226 U227 Trichloroethylene Trichlorofluoromethane 1,3,5-Trinitrobenzene Tris(2,3-dibromopropyl) phosphate Trypan blue Uracil mustard Vinyl chloride U043 Warfarin, and salts Xylenes Zine phosphide (<10%) U228 U121 U234 U235 U236 U237 U248 U239 U249 15

VIII. City Of Tyler Drain Discharge Prohibitions The following prohibitions are taken from City Ordinance # O-964, I-24-96, Sec. 19-73. A. General Prohibitions The following general chemical categories are not permitted to be discarded down the drain: Explosives Flammables Toxics/poisons Aqueous with ph <5.5 or >10.5 Petroleum products Radioactive materials B. Specific Prohibitions The following chemicals in excess of the maximum daily limits listed below are not permitted to be discarded down the drain: Chemical Regulatory Level (mg/l)* Arsenic 0.60 Cadmium 0.20 Chromium 5.00 Copper 0.60 Cyanides 0.30 Lead 0.30 Mercury 0.01 Nickel 2.00 Silver 0.10 Total phenols 2.00 Zinc 2.00 * Concentrations apply at the point where the waste is discharged to the POTW unless otherwise permitted. All concentrations for metallic substances are for total metal unless indicated otherwise. 16

IX. FORMS The following forms may be filled out and sent via campus mail or electronically to request a pickup of material. 17

A. Chemical Waste Accumulation Log Waste Log Number Date: Dept: Bldg Room Extension: This log sheet is required for each waste container. The Chemical name (spelled out, no abbreviations or structures), CAS#, solvent (if applicable), quantity and concentration must be listed as each waste is added to the container. Make sure that all wastes are chemically compatible before placing in the container. Use only chemically compatible containers and labeled properly. Wastes must be segregated according to the type of hazard present. The proper name for all wastes will be Waste followed by the chemical name. Use a waste label to identify the waste, the location, the log sheet number, and the responsible person. The generator is responsible for the identification of the waste. However, if the generator cannot positively identify the waste, the waste will be sent to a contract laboratory for analysis and the generator will be billed for the analytical costs. When the waste container is full call the EH&S office at 566-7011 and tape a copy of this waste log to the container. Chemical name and CAS# Solvent % CHEMICAL AND PHYSICAL HAZARDS Flammable Oxidizer Poison Corrosive Reactive Special Hazards WASTE CHARACTERIZATION ph Halogenated solvent? [ ] Yes [ ] No PRECAUTIONS OTHER THAN GLOVES/GOGGLES/APRON: I hereby certify that the above information is complete and accurate to the best of my knowledge and ability to determine and that there is no deliberate or willful omission. Name (print) Title: Signature: Date: 18

B. Sample Chemical Waste Accumulation Log Waste Log Number EH&S 001 Date: _Jan 23, 2003 Dept:_EH&S Bldg PHY Room 136b_Extension: 7011 This log sheet is required for each waste container. The Chemical name (spelled out, no abbreviations or structures), CAS#, solvent (if applicable), quantity and concentration must be listed as each waste is added to the container. Make sure that all wastes are chemically compatible before placing in the container. Use only chemically compatible containers and labeled properly. Wastes must be segregated according to the type of hazard present. The proper name for all wastes will be Waste followed by the chemical name. Use a waste label to identify the waste, the location, the log sheet number, and the responsible person. The generator is responsible for the identification of the waste. However, if the generator cannot positively identify the waste, the waste will be sent to a contract laboratory for analysis and the generator will be billed for the analytical costs. When the waste container is full call the EH&S office at 566-7011 and tape a copy of this waste log to the container. Chemical name and CAS# Solvent % Methanol, 67-56-1 20 Acetone, 67-64-1 30 Cyclohexane, 110-82-7 50 CHEMICAL AND PHYSICAL HAZARDS Flammable Oxidizer Poison Corrosive Reactive Special Hazards WASTE CHARACTERIZATION ph Halogenated solvent? [ ] Yes [ ] No PRECAUTIONS OTHER THAN GLOVES/GOGGLES/APRON: I hereby certify that the above information is complete and accurate to the best of my knowledge and ability to determine and that there is no deliberate or willful omission. Name (print) Title: Signature: Date: 19

C. Chemical Waste Label The University of Texas at Tyler Satellite Accumulation Waste Log No. Date: Dept: Bldg Room CHEMICAL AND PHYSICAL HAZARDS Flammable Oxidizer Poison Corrosive Reactive Special Hazards Constituent % Call EH&S at 903-566-7011 for pickup. UT Tyler, 3900 University Blvd., Tyler, TX 75799 Templates for printing small (Avery Label #5294) and large (Avery label #5164) are available from EH&S and departments are encouraged to use them. D. Sample Chemical Waste Form The University of Texas at Tyler Satellite Accumulation Waste Log No. EH&S 001 Date: Jan 23,2003 Dept: _EH&S Bldg PHY 136 Room_136b CHEMICAL AND PHYSICAL HAZARDS Flammable Oxidizer Poison Corrosive Reactive Special Hazards Constituent % Ethanol, denatured 20 Acetone 30 Cychohexane 50 Call EH&S at 903-566-7011 for pickup. UT Tyler, 3900 University Blvd., Tyler, TX 75799 20

Date: E. BioWaste Collection Form Contact Person: Department: Collection Site: Phone #: (Building/Room #) I certify that the following information is correct to the best of my knowledge, information and belief and that the biomedical waste is in a proper container for handling and storage. Signature of Authorized Delegate Biomedical Waste Type / Container Type Quantity 21