Molecular Insect Physiology Laboratory



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Molecular Insect Physiology Laboratory Qisheng Song, Ph.D. Associate Professor Molecular Insect Physiology Division of Plant Sciences University of Missouri Columbia, MO 65211 Phone: 573-882-9798 (O) 573-882-8346 (L) Fax: 573-882-1469 Email: songq@missouri.edu Educational Background 1989-92 Ph.D. Department of Entomology, University of Maryland, College Park, MD 1986-88 M.S. Department of Entomology, University of Maryland, College Park, MD 1978-82 B.S. Department of Plant Protection, Zhejiang University, Hangzhou, China Professional Appointment 2006-present Associate Professor, Insect Physiology, Division of Plant Sciences University of Missouri, Columbia, MO 2000-2005 Assistant Professor, Molecular Insect Physiology, Department of Entomology University of Missouri, Columbia, MO 1992-1999 Post-Doctoral Research Associate, Department of Biology, University of North Carolina, Chapel Hill, NC 1986-1992 Graduate Research Assistant, Department of Entomology, University of Maryland, College Park, MD 1983-1985 Teaching and Research Assistant, Department of Plant Protection, Zhejiang University, Hangzhou, Zhejiang, China Editorial Board Member 1. Archives of Insect Biochemistry and Physiology, 2005-present 2. Journal of Economic Entomology, ESA Integrative Physiological and Molecular Insect Systems (IPMIS) representative, 2006-present 3. Acta Entomologica Sinica, 2007-present Selected Publications An SH, Wang SJ, Gilbert LI, Beerntsen B, Ellersieck M and Song Q (2008) Global identification of bursicon-regulated genes in Drosophila melanogaster. BMC Genomics 9, 924 (doi:10.1186/1471-2164-9-424). An SH, Wang SJ, Stanley D and Song Q (2008) Identification of a novel bursicon-regulated transcriptional regulator, md13379, in the house fly Musca domestica. Arch Insect Biochem Physiol (in press). 1

Feng CJ, Song Q, Lu WJ and Lu JF(2008) Purification and characterization of hemolymph prophenoloxidase from Ostrinia furnacalis (Lepidoptera: Pyralidae) larvae. Comp Biochem Physiol - Part B 151, 139-146. Stanley D, Goodman C, An SH and Song Q (2008) Prostaglandins A1 and E1 influence gene expression in an established insect cell line (BCIRL-HzAM1 cells). Insect Biochem Molec Biol 38, 275-284. Wang SJ, An SH and Song Q (2008) Bursicon mediates expression of a specific set of genes in the house fly Musca domestica. Arch Insect Biochem Physiol 68, 100-112. Ye GY, Dong SZ, Song Q, Shi M, Chen XX and Hu C (2008) Molecular cloning and developmental expression of the vitellogenin gene in the endoparasitoid, Pteromalus puparum. Insect Mol Biol 17, 227-233. Sun YN, An SH, Henrich VC, Sun XP and Song Q (2007) Proteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogaster. J Proteome Research 6, 4478-4488. Li H, Sites RW and Song Q (2007) Recovery of mitochondrial and nuclear DNA for systematic studies of Naucoridae (Heteroptera): an assessment of preservation techniques and specimen age. Insect Syst Evol 38, 167-172. Sun X and Song Q (2006) PKC-mediated USP phosphorylation is required for 20E-induced gene expression in the salivary glands of Drosophila melanogaster. Arch Insect Biochem Physiol 62, 116-127.. Song Q and Sun X (2005) Hormonal control of insect growth and development (Invited book chapter). In Entomological Research: Progress and Perspective. Tong-Xian Liu and Kang Le [Ed]. Science and Technology Press (China), pp 89-123. Jin X, Sun X. and Song Q (2005) Woc gene mutation causes 20E-dependent α-tubulin detyrosination in Drosophila melanogaster. Arch Insect Biochem Physiol 59, 116-129. Liu X, Tanaka Y, Song Q, Xu B, Hua Y (2004) Bombyx mori prothoracicostatic peptide inhibits ecdysteroidogenesis in vivo. Arch Insect Biochem Physiol 56, 155-61. Sun X, Barrett BA and Song Q (2004) Effects of age and length of exposure on the reproduction of adult Codling moth exposed to surfaces treated with ecdysone. J Entomol Sci 39, 417-425. Song Q, Sun X and Jin X (2003) 20E-regulated USP phosphorylation in Drosophila melanogaster. Insect Biochem Molec Biol 33, 1211-1218. Sun X, Song Q and Barrett BA (2003) Effect of ecdysone agonists on vitellogenesis and the expression of EcR and USP in codling moth (Cydia pomonella). Arch Insect Biochem Physiol 52, 115-129. Sun X., Song Q and Barrett BA (2003) Effect of ecdysone agonists on the expression of EcR/USP complex-mediated specific proteins in the ovary of Codling moth (Cydia pomonella) Insect Biochem Molec Biol 33, 829-840. Gilbert LI, Rybczynski R, Song Q, Mizoguchi A, Morreale R, Smith WA, Matubayashi H, Shionoya M, Nagata S and Kataoka H (2000) Dynamic regulation of prothoracic gland ecdysteroidogenesis: Manduca sexta recombinant prothoracicotropic hormone and brain extracts have identical effects. Insect Biochem Molec Biol 30, 1079-1089. Song Q and Gilbert LI (1998) Alterations in Ultraspiracle (USP) content and phosphorylation state accompany feedback regulation of ecdysone synthesis in the insect prothoracic gland. Insect Biochem Molec Biol 28, 849-860. Gilbert LI, Song Q and Rybczynski R (1997) Control of ecdysteroidogenesis: activation and inhibition of prothoracic gland activity. Invertebrate Neuroscience 3, 205-216. Song Q, Alnemri ES, Litwack G and Gilbert LI (1997) An immunophilin is a component of the insect ecdysteroid receptor (EcR) complex. Insect Biochem Molec Biol 27, 973-982. Song Q and Gilbert LI (1997) Molecular cloning, developmental expression, and phosphorylation of ribosomal protein S6 in the endocrine gland responsible for insect molting. J Biol Chem 272, 4429-4435. 2

Song Q and Gilbert L I (1996) Protein phosphatase activity is required for prothoracicotropic hormonestimulated ecdysteroidogenesis in the prothoracic glands of tobacco hornworm, Manduca sexta. Arch Insect Biochem Physiol 31, 465-480. Song Q and Gilbert LI (1995) Multiple phosphorylation of ribosomal protein S6 and specific protein synthesis are required for prothoracicotropic hormone-stimulated ecdysteroid biosynthesis in the prothoracic glands of Manduca sexta. Insect Biochem Molec Biol 25, 591-602. Song Q and Gilbert LI (1994) S6 phosphorylation results from prothoracicotropic hormone stimulation of insect prothoracic glands: a role for S6 kinase. Dev Genetics 15, 332-338. Song Q, Combest W and Gilbert LI (1994) Spermine and polylysine enhanced phosphorylation of calmodulin and tubulin in an insect endocrine gland. Mol Cell Endocrinol 99, 1-10. Borovsky D, Song Q, Ma M and Carlson DA (1994) Biosynthesis, secretion, and immunocytochemistry of trypsin modulating oostatic factor of Aedes aegypti. Arch Insect Biochem Physiol 27, 27-38. Song Q and Gilbert L (1993) Calmodulin and Casein Kinase II in the brain and prothoracic gland of Manduca sexta. In Insect Neurochemistry and Neurophysiology, III (Edited by Loeb, M.J. and Borkovec, A.B.), pp149-152. CRC Press Inc., Boca Raton, Florida. Song Q and Ma M (1992) A monoclonal antibody to a 56 kda neurosecretory polypeptide from the posterior lateral neurosecretory cells of the house fly, Musca domestica. Insect Biochem Molec Biol 22, 149-157. Ballarino J, Song Q, Ding T, Ma M and Chang F (1991) Effects of benzodioxole on microtubule formation in the ovaries of Drosophila melanogaster. Pest Biochem Physiol 41, 258-264. Song Q, Ma M, Ding T, Ballarino J and Wu S-J (1990) Effect of benzodioxole, J2581 (5-ethoxy-6-(4- methoxyphenol) methyl-1,3-benzodioxole, on vitellogenesis and ovarian development of Drosophila melanogaster. Pest Biochem Physiol 37, 12-23. Song Q and Ma M (1990) Anti-gonodotropic effects of benzyl-1,3-benzodioxole (J2581) on Drosophila melanogaster. Bull Inst Zool, Acdemia Sinica. Monograph 15, 71-86. Ma M, Beier JC, Petrarca V, Gwadz RW, Zhang J-Z, Song Q and Koech DK (1990) Differentiation of Anopheles gambiae and An. arabiensis (Diptera: Culicidae) by ELISA using immunoaffinitypurified antibodies to vitellogenin. J Med Entomol 27, 564-569. Hu C and Song Q (1985) Studies on the field distribution of Acaphila theae: temporal distribution. Tea 5, 12-14. Hu C and Song Q (1985) Studies on the field distribution of Acaphilla theae (Watt): Spacial distribution. Acta Agri Univ Zhejiangensis 11, 395-401. Hu C, Zhao Q, Ren X and Song Q (1985) Studies on the distribution off Acaphylla theae in tea field. Bulletin of Science and Technology 4, 33. Hu C and Song Q (1984) The sources and applications of natural enemies in tea pest control (review). Tea 4, 45-49. Invited Presentations Song Q (2008) The roles of PKC in mediating 20E-induced gene expression in salivary glands of Drosophila melanogaster. Section 12: Behaviour and Neurobiology: Signaling by G protein-coupled receptor in arthropods. The XXIII International Congress of Entomology, Durban, South Africa. Song Q (2007) Microarray analysis of bursicon-regulated genes in Drosophila (keynote speaker). The 8 th National Congress of Entomology Society of China. Zhengzhou, Henan. Song Q (2007) Signal transduction: from molting to cuticle hardening (keynote speaker). International Symposium on Insect Physiology, Biochemistry and Molecular Biology. Jinan, Shandong, China. Song Q (2007) Proteomic identification of molting hormone-induced proteins in the salivary gland of Drosophila melanogaster. China Agriculture University, Beijing, China. 3

Song Q (2005) Molting hormone signal transduction. Institute of Applied Entomology, Zhejinag University, Hangzhou, China. Song Q (2005) Hormonal signal transduction: from PTTH to molting hormone. College of Life Sciences, Shandong University, China. Song Q (2004) The role of protein phosphorylation in regulating the function of ecdysone receptor complex. Insect Signal Transduction Systems Symposium: Current Knowledge & Future Directions. Section 12: Neurobiology & Behavior: Linking Molecular Biology, Developmental Biology & Behavior. The XXII International Congress of Entomology, Brisbane, Australia. Song Q (2004) Roles of protein kinase C-mediated EcR/USP phosphorylation in regulating the expression of 20E responsive proteins in Drosophila. Department of Biology, University North Carolina- Greensboro. Song Q (2003) Hormonal signal transduction: from PTTH to ecdysteroids. Department of Entomology, Kansas State University. Song Q (2003) PTTH signaling pathway in Manduca sexta. Department of Entomology, University of Maryland. Song Q (1999) PTTH signaling pathway in Manduca sexta. Department of Plant Protection, Zhejiang University, Hangzhou, China. Song Q (1999) PTTH signal transduction. Department of Entomology, University of Maryland, College Park, MD. Song Q (1998) The role of S6 phosphorylation in PTTH-stimulated ecdysteroidogenesis. University of Tokyo, Tokyo, Japan. Song Q (1998) The involvement of ecdysone receptor complex in regulating PTTH-stimulated ecdysteroid synthesis in the prothoracic gland of Manduca sexta. University of Nagoya, Nagoya, Japan. Song Q (1998) Stimulatory and feedback regulation of ecdysteroid synthesis. National Institute of Sericulture and Entomological Science, Tsukuba, Japan. Song Q (1998) Control of ecdysteroidogenesis: activation and inhibition of prothoracic gland activity. University of Kobe, Kobe, Japan. Song Q and Gilbert LI (1997) The neuroendocrinology of molting: from neuropeptide to ecdysone receptor. The Fifth International Conference on Invertebrate Neurochemistry & Neurophysiology (ICINN), Eilat, Israel. Professional Service 1. President, Overseas Chinese Entomologists Association (OCEA), 2008 2. Vice president, Overseas Chinese Entomologists Association (OCEA), 2007 3. Treasurer, Overseas Chinese Entomologists Association (OCEA), 2004-2006 4. Entomological Society of America (ESA) Common Name Committee, 2005-2007 5. Faculty Development Advisory Committee, University of Missouri, 2007-present 6. MU Division of Plant Sciences, Research Incentive Fund Committee, 2007-present 7. MU Division of Plant Sciences Seminar Committee, 2007-present 8. MU Entomology Program Gilbreath-Mclorn Fund Committee, 2006-present 9. Symposium Academic Committee Member, the 7 th International Symposium on Insect Physiology, Biochemistry and Molecular Biology, 2007 10. Guest Editor: a special issue of Archives of Insect Biochemistry and Physiology for International Symposium on Insect Physiology, Biochemistry and Molecular Biology, 2008 11. Symposium organizer: Frontiers in Molecular Insect Science. Entomological Society of America Annual Conference, Reno, 2008 Professional Membership 1. American Association for the Advancement of Science (AAAS) 4

2. Entomology Society of America (ESA) 3. Genetic Society of American 4. C.V. Riley Entomological Society 5. Overseas Chinese Entomologists Association Teaching 1. Principles of Insect Physiology-Plant Sciences 7820 (4 credit hours with a laboratory session). Spring, even year. 2. Insect Toxicology-Plant Sciences 9820 (3 credit hours). Spring, odd year. 3. Seminar in Plant Science-Plant Sciences 9087 (1 credit hour). Co-instructor. 5