QUALITY AND SAFETY TESTING Large scale of solutions for the identification and rapid detection of micro-organisms. Easier investment in molecular techniques Frédéric BAR, Key Account Manager
b2 b3b4 Quality & Safety Testing b5 Une histoire: l origine de la vie Un saut: des technologies accessibles pour le contrôle de routine 1981 Applied Biosystems is founded. 1983 AB introduce s 380A DNA synthe sizer. 1998 AB introduce s ABI PRISM 3700 DNA Analyzer: accelerating completion of human genome draft. 2002 2004 AB DNA analyzers and reagents used to identify 30,000 human genes and discover 10 million+ unique SNPs. 1981 1983 1998 2002 2004 1982 1987 2000 2003 2005 AB introduce s first First commercial PCR AB DNA analyzers AB DNA analyzers, gas-pha se protein enzyme and thermal and reagents real-time PCR, and sequencer: the 470A. cycler systems enable draft protein ID systems introduced. version of the used to sequence human genome the SARS virus, and sequence. to develop diagnostic te sts. 2 15-Sep-06 Confidential
Diapositive 2 b2 Linda - I shortened the words...i really like this slide! Cathy burzikcm; 09/01/2005 b3 burzikcm; 09/01/2005 b4 burzikcm; 09/01/2005 b5 Linda- I really like this slide...i just shortened it. Cathy burzikcm; 09/01/2005
Applied Markets Market Segments Forensic Human Identification Quality & Safety Testing (QST) Pharma & Food & Environmental Biosecurity On-line pathogen detection Toxicology Microbial Identification Metabolite analysis 3 15-Sep-06 Confidential
Marché de la détection et de l identification Market Value of Food Testing Pathogen Tests by Method USD Millions 1,500 1,309 Market size and growth In 2003 the market size for food pathogen testing s $490M 100% 80 $420M $490M $968M Traditional 1,000 500 0 639 760 Pathogen 968 Routine 341 2001 2003 2008 $100M (20%) is comprised of molecular methods. Molecular methods are the fastest growing of all methods used for pathogen testing and are expected to grow to over $360M in 2008 (2003-2008 CAGR = 29%). 60 40 20 0 Immunoassay Molecular 2001 2008 38% of Total 4 15-Sep-06 Confidential
Notre Stratégie Bioinformatics Content Celera Discovery Systems TM Platform Assays Reagents and Assays Instruments Results! Platforms 5 15-Sep-06 Confidential Services Services & Informatics
Why DNA testing? Antibody reactions DNA RNA Proteins Metabolic reactions Genotypic vs. Phenotypic Fatty acid profiles 6 15-Sep-06 Confidential
Microbiologie Alimentaire De Pasteur à la PCR en temps réel 4 différents TaqMan Pathogen Detection Kits: Listeria monocytogenes (hlya gene) Salmonella enterica (hila gene) Escherichia coli 0157:H7(eaeA gene) Campylobacter jejuni (ccon gene) Environe ment Legionella spp et pneumophila Pharma et Cosmétique QC Staphylococcus aureus Pseudomonas aeruginosa ADN résiduel Mycoplasme Vins et Bières Virus Brettanomyces (Prédel) Grippe Aviaire H5N1 Rotavirus, Norovirus, 7 15-Sep-06 Confidential
De Pasteur au séquençage MicroSEQ: Solution pour l identification Bactérie/moisissure Identification à 100% 8 15-Sep-06 Confidential
MicroSeq System Workflow Starting material: sample from colony or pure culture DNA isolation with PrepMan Ultra kit 30 min PCR with fast MicroSeq PCR kit Purification of PCR product Sequencing reaction with MicroSeq sequencing kit (2 reactions / sample: forward and reverse) Purification of sequencing product Capillary electrophoresis on Genetic Analyzer 20 min 50 min 2 hours Data analysis in MicroSeq ID software 10 min 9 15-Sep-06 Confidential Library Search Report (Hit List)
Gamme d instrument la plus large Haut débit Developpement 5 couleurs 384 puits Prix New QC 3 couleurs 48 puits pilotable par Internet Ecran tactile 10 15-Sep-06 Confidential 4-5 couleurs 96 puits Caractéristiques Laser Robotisé Product Price List ( ) StepOne 21 750 7300 34 900 7500 42 500 7500 Fast 49 500 7900HT 76 500 7900HT LDA 89 000 7900HT R 108 800
Nouvelle stratégie d investissement pour les laboratoires d analyses Organisation Multi travail Screening Haut débit TTR Spécificité ADN Investissement initial lourd Méthode traditionnelle Coût analyse élevé Certification Méthode moléculaire Acquisition connaissance 11 15-Sep-06 Confidential
Investissement initial Haut débit Diagnostic m2000rt Developpement 7500 Fast 384- Well Feature Loaded 7900HT 7900HTA 7300 QC 4-Color 7300 5-Color 7500 Prix 3-Color StepOne Single Reporter 12 15-Sep-06 Confidential Caractéristiques Product Price List ( ) StepOne 21 750 7300 34 900 7500 42 500 7500 Fast 49 500 7900HT 76 500 7900HT LDA 89 000 7900HT R 108 800
Investissement initial Haut débit 1 StepOne m.a.d. 20 000 Analyses/an Diagnostic m2000rt Developpement 7500 Fast 384- Well Feature Loaded 7900HT 7900HTA 7300 QC 4-Color 7300 5-Color 7500 Volume Prix Analyses 3-Color StepOne Single Reporter 13 15-Sep-06 Confidential Caractéristiques
Coût d analyse Segmentation des clients Industries Pharma Cosméto IAA RHF Techni que moléculaire = protection de l a marque Concentration des pathogènes sur 1 plaque Moins de lecture = réduction du te mps de travail Automatisation 14 15-Sep-06 Confidential
Applied Biosystems Efficient Route to Success Quality & Safety Testing Un support complet-formations-séminaires Screen Validated Targets Validate with TaqMan High Quality DNA Determine faster& accurate microbial burden Genetic analysers 15 15-Sep-06 Confidential