P/ACE MDQ Basic Taining Wokbook 32 Kaat 8.0 A33116AB Novembe 2009 Beckman Coulte, Inc. 250 S. Kaeme Blvd., Bea, CA 92821 Copyight 2009 Beckman Coulte, Inc.
Copyight, Licenses and Tademaks Copyight Beckman Coulte, Inc., 2009. All ights eseved. No pat of this publication may be epoduced, tanscibed, tansmitted, o tanslated into any language in any fom by any means without the witten pemission of Beckman Coulte, Inc. Licenses and Tademaks Beckman Coulte is a egisteed tademak of Beckman Coulte, Inc. P/ACE MDQ is a tademak of Beckman Coulte, Inc. 32 Kaat Softwae is a tademak of Beckman Coulte, Inc. Micosoft and Windows ae egisteed tademaks of Micosoft Copoation.
Table of Contents Section 1 System Oveview.............................. 1 Oveview............................................................1 Hadwae Teminology.......................................................... 2 Tays........................................................................ 3 Capillay Catidge.............................................................. 4 Detectos (UV, PDA o LIF)....................................................... 5 Softwae Teminology........................................................... 5 Summay..................................................................... 6 Section 2 Safety...................................... 7 Oveview............................................................7 Safety Infomation fo P/ACE MDQ and 32 Kaat Softwae......................7 Safety Featues................................................................ 7 Safety Notices................................................................. 7 Chemical and Biological Safety.................................................... 8 Electical Safety................................................................ 8 Electostatic Dischage.......................................................... 8 Release Notes................................................................. 8 Summay..................................................................... 8 Section 3 Softwae Setup and Initialization................... 9 Oveview............................................................9 Accessing 32 Kaat Softwae.............................................9 Instument Configuation..............................................11 Module Configuation: Selecting Options..................................12 Stating the Newly Configued Instument..................................14 Instument Wizad............................................................. 14 Instument Window Sceen Layout................................................ 14 Skill Check..........................................................15 Summay...........................................................15 Section 4 Reviewing Detectos........................... 17 Oveview...........................................................17 UV Detecto.........................................................17 UV Initial Conditions........................................................... 17 Electopheogam Channel...................................................... 18 Filte Settings................................................................ 18 Relays...................................................................... 18 Absobance signal............................................................. 18 UV Data Display............................................................... 18 PDA Detecto........................................................19 iii
PDA Initial Conditions.......................................................... 19 Electopheogam scan data..................................................... 19 Electopheogam channel data................................................... 19 Filte Settings................................................................ 19 Faction Collecto /Relays....................................................... 20 Refeence Channel............................................................. 20 Absobance signal............................................................. 20 PDA Setup..........................................................20 PDA Data Display.....................................................24 View Options................................................................. 25 LIF Detecto.........................................................26 LIF Initial Conditions........................................................... 26 Electopheogam Channels..................................................... 26 Lase/Filte Desciptions........................................................ 26 Data Rate...........................................................27 Relays...................................................................... 27 LIF Data Display............................................................... 27 LIF Calibation Wizad.......................................................... 28 Summay...........................................................29 Section 5 Using Diect Contol........................... 31 Oveview...........................................................31 Accessing Diect Contol........................................................ 31 Using Diect Contol..................................................32 Catidge Coolant Tempeatue................................................... 32 Sample Stoage Tempeatue.................................................... 32 Capillay Infomation........................................................... 32 Rinse....................................................................... 33 Inject....................................................................... 33 Sepaate.................................................................... 34 Detecto Settings.............................................................. 34 UV Detecto Paametes........................................................ 34 PDA Detecto Paametes....................................................... 34 LIF Detecto Paametes........................................................ 35 Lamp Status................................................................. 35 Lase Status................................................................. 35 Tay Position................................................................. 35 Voltage Settings.............................................................. 35 Cuent Settings.............................................................. 35 Powe Settings............................................................... 36 Pessue.................................................................... 36 Time Remaining.............................................................. 36 Status...................................................................... 37 Skill Check..........................................................38 Summay.................................................................... 38 iv
Section 6 Woking with Methods.......................... 39 Oveview...........................................................39 Using the Method Wizad....................................................... 39 Ceating a Method............................................................. 40 Instument Setup.............................................................. 41 Initial Conditions Tabs.......................................................... 42 Time Pogamming............................................................ 43 Events...................................................................... 44 UV Detecto.................................................................. 44 PDA Detecto................................................................. 44 LIF Detecto.................................................................. 44 Extenal Detecto.............................................................. 44 Saving a Method.............................................................. 45 Editing a Method.............................................................. 46 Pinting a Method............................................................. 47 Othe Method Functions Popeties................................................47 Skill Check..........................................................50 Summay.................................................................... 50 Section 7 Running A Sample............................ 51 Oveview...........................................................51 Running a Single Sample..............................................51 Stopping / Aboting Method............................................52 Displaying Data......................................................53 Add Tace................................................................... 54 Add Multiple Taces............................................................ 55 Axis Setup................................................................... 56 Annotations.................................................................. 57 Appeaance.................................................................. 57 Full Unzoom................................................................. 58 Utilities..................................................................... 59 Popeties................................................................... 59 Skill Check..........................................................60 Summay.................................................................... 60 Section 8 Analyzing and Integating Data.................... 61 Oveview...........................................................61 Opening Data Files....................................................61 Gaphical Pogamming........................................................ 62 Analyzed electopheogam...................................................... 63 Optimizing Integation.................................................64 Defining and Naming Peaks.............................................66 Identifying Peaks based on Migation Time.......................................... 67 Identifying Peaks based on Mobility............................................... 71 Skill Check..........................................................73 v
Summay.................................................................... 73 Section 9 Using Sequence Tables......................... 75 Oveview...........................................................75 Using the Sequence Wizad.............................................75 Viewing a Sequence...................................................79 Editing a Sequence...................................................81 Saving a Sequence...................................................82 Running a Sequence..................................................83 Skill Check..........................................................86 Summay.................................................................... 86 Section 10 Ceating Calibations.......................... 87 Oveview...........................................................87 Editing the Peak ID Table...............................................87 Paamete Options............................................................. 88 Ceating a Calibation Sequence with the Sequence Wizad....................89 Running a Calibation Sequence.........................................91 Reviewing Calibation Cuves...........................................92 Final Skill Check......................................................94 Summay.................................................................... 94 Section 11 Pepaing Custom Repots....................... 95 Oveview...........................................................95 Accessing and Editing a Custom Method Repot.............................95 Ceating a Custom Method Repot.......................................98 Skill Check.........................................................103 Summay................................................................... 103 Section 12 Summay.................................. 105 Advancing You Skill.................................................105 Recod of Opeato Taining...........................................106 Taining Summay............................................................ 106 Checklist:................................................................... 107 Signatues:................................................................. 108 vi
Foewod Welcome Foewod Welcome Welcome to the Basic level of the Beckman Coulte taining pogam fo P/ACE MDQ with 32 Kaat Softwae. Duing this taining, ou enginee will guide you though the basic opeation of the system, eview safety and maintenance guidelines, and eview optional accessoies that povide optimum pefomance fo you system. The pupose of basic taining is to intoduce you to the system and ensue that you ae able to pefom basic functions. Howeve, the taining is not intended to include ceating custom applications. Fo advanced taining options, please contact you sales office. The following peequisites have been defined to ensue a successful basic taining: 1. The opeato must be available without inteuption fo the entie taining session. 2. No moe than two opeatos will be tained as pat of the installation. The system opeation equies a basic knowledge of Windows XP. The opeato must be familia with the following tems and skills: Ceating, opening, saving, editing, moving, and copying files P/ACE MDQ Taining Wokbook PN A33116AB vii
Foewod Taining Oveview Taining Oveview The taining is oganized in thiteen sections. You instucto will guide you though each section. At the end of most sections, a skill check is povided to help you evaluate you pogess. System Oveview Oveview Hadwae Teminology Softwae Teminology Safety Safety Featues Safety Notices Chemical and Biological Safety Electical Safety Electostatic Dischage Softwae Setup and Initialization Accessing 32 Kaat Softwae Configuing 32 Kaat Softwae Stating the Instument Skill Check Reviewing Detectos UV Detectos PDA Detectos LIF Detectos Using Diect Contol Accessing Diect Contol Using Diect Contol Sceens Skill Check Woking with Methods Using the Method Wizad Ceating a Method Saving a Method Editing a Method Pinting a Method Othe Method Functions Skill Check viii 32 Kaat 8.0 PN A33116AB
Foewod Taining Oveview Running a Sample Running a Single Sample Stopping o Aboting a Method Displaying Data Skill Check Analyzing and Integating Data Opening Data Files Optimizing Integation Defining and Naming Peaks Identifying Peaks Based on Migation Time Identifying Peaks Based on Mobility Skill Check Using Sequence Tables Using the Sequence Wizad Viewing a Sequence Editing a Sequence Saving a Sequence Running a Sequence Skill Check Ceating Calibations Editing the Peak ID table Ceating a Calibation Sequence with the Sequence Wizad Running a Calibation Sequence Reviewing Calibation Cuves Final Skill Check Pepaing Custom Repots Accessing and Editing a Custom Method Repot Ceating a Custom Method Repot Skill Check Summay Advancing you skill Recod of Opeato Taining P/ACE MDQ Taining Wokbook PN A33116AB ix
Foewod Taining Oveview x 32 Kaat 8.0 PN A33116AB
TM System Oveview Oveview 1 1System Oveview 1.1 Oveview This section descibes the hadwae components of P/ACE MDQ system. You will lean the opeation consideations fo each module and tems commonly used fo the softwae and hadwae. PWP UV HV + HV - P/ACE MDQ 901004L.AI Figue 1.1 P/ACE MDQ System P/ACE MDQ Taining Wokbook PN A33116AB 1
System Oveview Oveview Hadwae Teminology System Powe Switch Font Panel LEDs and Indicato lights Communications cables Remote hook-ups Othe detectos Spae pats and consumables list 2 32 Kaat 8.0 PN A33116AB
System Oveview Oveview 1 Tays Tay Cove and Capillay Catidge Cove Tay Racks Sample Tay and Sample Cooling Tay Name and Fomat 12 6 11 5 10 1 A 4 B C D E F 3 3 2 1 1 2 3 6 9 8 7 5 4 8 A B C D E F G H 7 6 5 4 2 4 3 2 1 A B C D E F 901018L.AI 1. Buffe Tay 2. 48 Vial Sample Tay 3. 96 Position Sample Tay 4. Lage Volume Buffe Resevoi P/ACE MDQ Taining Wokbook PN A33116AB 3
System Oveview Oveview Capillay Catidge 6 1 7 5 2 3 4 901011L.AL 1. Coolant tubing with capillay inside 2. Inlet Side 3. Capillay 4. Outlet Side 5. Detecto Window and Apetue 6. Apetue fo UV and PDA catidge 7. Apetue and Stabilize Plug fo LIF catidge 4 32 Kaat 8.0 PN A33116AB
System Oveview Oveview 1 Detectos (UV, PDA o LIF) Lamp Hous Change pocedue Diagnostics Softwae Teminology Window 2 3 4 Title Ba Menu Ba Toolba Status Line P/ACE MDQ Taining Wokbook PN A33116AB 5
System Oveview Oveview Main Menu New Instument System Configuation Inteface Configuation Online/Offline System Administation Summay This section intoduced you to an oveview of P/ACE MDQ system. Now you ae eady to lean the details equied fo successful opeation. Refe to "Safety" on page 2-7 befoe poceeding. 6 32 Kaat 8.0 PN A33116AB
Safety 2 Oveview 2Safety 2.1 Oveview This section povides safety instuctions fo P/ACE MDQ hadwae and accessoies. You will eview: Safety Featue Safety Notices Chemical and biological safety Electical safety Moving pats Instument safety featues Release Notes 2.2 Safety Infomation fo P/ACE MDQ and 32 Kaat Softwae All safety instuctions should be ead and undestood befoe installation, opeation, maintenance o epais ae attempted. See the MDQ Use s Guide fo Safety Infomation egading this system. Safety Featues Review the location and action of the following safety featues: B ON/OFF switch B Fuse eplacement and voltage selection Safety Notices Review meaning and placement: B Intenational safety symbols B High voltage symbol P/ACE MDQ Taining Wokbook PN A33116AB 7
Safety Safety Infomation fo P/ACE MDQ and 32 Kaat Softwae Chemical and Biological Safety Nomal opeation of the system involves the use of many solvents and eagents, which may be toxic, flammable o biologically hamful. B Obseve all cautionay infomation pinted on the oiginal solution containes pio to use. B Opeate the system in an appopiate enclosue and take all necessay pecautions when using pathologic, toxic, o adioactive mateials to pevent the geneation of aeosols. B Obseve the appopiate cautionay pocedues as defined by you safety office when using flammable solvents in o nea the poweed-up instument. B Wea appopiate lab attie (safety glasses, gloves, lab coat, and beathing appaatus) when woking with hazadous mateials. B Remembe that solvents may be flowing unde high pessue. Electical Safety B Always disconnect powe to the system befoe pefoming maintenance opeations. B Refe sevicing that equies emoval of coves to qualified pesonnel. Electostatic Dischage B Gound youself befoe woking with system. B Capeting in a building can influence static chage. Use caution when woking in this envionment. Contact Beckman Coulte Field Sevice if you have questions. Release Notes The Release Notes contain impotant infomation that became available afte the 32 Kaat Softwae manuals wee pinted: Read and eview the infomation contained in this file Pint the file and place in binde, if necessay Summay This completes the safety potion of the basic taining. Fo moe detailed infomation egading safety, efe to the P/ACE MDQ Installation and Maintenance Guide. WARNING Do not use the poduct in any manne othe than specified. The safety and pefomance of the equipment will be impaied. 8 32 Kaat 8.0 PN A33116AB
Softwae Setup and Initialization Oveview 3 3Softwae Setup and Initialization 3.1 Oveview This section consides the stat-up pocess fo the 32 Kaat Softwae. We will discuss: Accessing 32 Kaat Softwae The Entepise Sceen (Main Menu) Configuing 32 Kaat Softwae Sceen Layout Skill Check 3.2 Accessing 32 Kaat Softwae Figue 3.1 Accessing 32 Kaat Softwae though Statup Menu P/ACE MDQ Taining Wokbook PN A33116AB 9
Softwae Setup and Initialization Accessing 32 Kaat Softwae Figue 3.2 Accessing 32 Kaat Softwae though Windows 10 32 Kaat 8.0 PN A33116AB
Softwae Setup and Initialization Instument Configuation 3 Figue 3.3 32 Kaat Softwae Entepise Sceen (Main Menu) B Ceating an Instument B Online Instuments (fo contol of system) B Offline Instuments (fo multitasking) B System Administation B System Administation Wizad B Uses 3.3 Instument Configuation Naming the Instument When and how to auto-configue Selecting system options SS420A/D Boad o SS420x A/D Device P/ACE MDQ Taining Wokbook PN A33116AB 11
Softwae Setup and Initialization Module Configuation: Selecting Options 3.4 Module Configuation: Selecting Options Naming the Detectos Figue 3.4 P/ACE MDQ System Configuation dialog Figue 3.5 Instument Configuation dialog GPIB Communication Inlet/Outlet Tays Sample Tays 12 32 Kaat 8.0 PN A33116AB
Softwae Setup and Initialization 3 Module Configuation: Selecting Options LIF Calibation Wizad Filtes Units Tempeatue Contol Figue 3.6 Configuation Options dialog PDA System Suitability Qualitative Analysis Caesa Integation P/ACE MDQ Taining Wokbook PN A33116AB 13
Softwae Setup and Initialization Stating the Newly Configued Instument 3.5 Stating the Newly Configued Instument Instument Wizad Figue 3.7 Instument Wizad dialog Instument Window Sceen Layout Figue 3.8 Instument Window Instument Window 14 32 Kaat 8.0 PN A33116AB
Softwae Setup and Initialization Skill Check 3 Title Ba Menu Ba Tool Ba Status Line 3.6 Skill Check Upon completion of this section, you should be able to do the following: 1. Stat the compute. 2. Log on to the Windows opeating system. 3. Stat 32 Kaat Softwae. 4. Ceate an Instument. 5. Auto Configue the instument. 6. Open the Instument Window. 3.7 Summay This completes the softwae setup potion of the taining. The softwae should now be stated and configued. The next section coves the vaious types of detectos. You will eview the infomation that petains to the configuation of you instument. Only one detecto type will be coveed. P/ACE MDQ Taining Wokbook PN A33116AB 15
Softwae Setup and Initialization Summay 16 32 Kaat 8.0 PN A33116AB
Reviewing Detectos Oveview 4 4Reviewing Detectos 4.1 Oveview Depending on the configuation of you system, the function of the detecto(s) vaies. This section focuses on the impotant setup and functions of each detecto. Some of these elate to epot options you will lean in Section 12. In this section we will discuss: UV Initial Conditions Tab UV Data Display PDA Initial Conditions Tab The PDA Setup Window PDA Data Display LIF Initial Conditions Tab LIF Data Display LIF Calibation Wizad 4.2 UV Detecto UV Initial Conditions Figue 4.1 UV Initial Conditions tab P/ACE MDQ Taining Wokbook PN A33116AB 17
Reviewing Detectos UV Detecto Electopheogam Channel Wavelength Filte Settings Data Rate Peak width Relays On/Off Absobance signal Diect Indiect UV Data Display Figue 4.2 Instument Window with UV data displayed 18 32 Kaat 8.0 PN A33116AB
Reviewing Detectos PDA Detecto 4 4.3 PDA Detecto PDA Initial Conditions Figue 4.3 PDA Initial Conditions tab Electopheogam scan data Data ate Scan ange Electopheogam channel data Data ate Filte Settings Channel Definition Peak detect Peak width P/ACE MDQ Taining Wokbook PN A33116AB 19
Reviewing Detectos PDA Setup Faction Collecto /Relays On Off Refeence Channel Wavelength Bandwidth Absobance signal Diect Indiect 4.4 PDA Setup Figue 4.4 PDA Options with Libay tab selected Libay Seach Paametes 20 32 Kaat 8.0 PN A33116AB
Reviewing Detectos PDA Setup 4 Figue 4.5 PDA Options with Puity tab selected Puity Calculations Figue 4.6 PDA Options with Spectum tab selected Spectal Filteing P/ACE MDQ Taining Wokbook PN A33116AB 21
Reviewing Detectos PDA Setup Figue 4.7 PDA Options with Multi-Electopheogam tab selected Enabled Wavelength Bandwidth 22 32 Kaat 8.0 PN A33116AB
Reviewing Detectos PDA Setup 4 Figue 4.8 PDA Options with Ratio tab selected Wavelength Bandwidth P/ACE MDQ Taining Wokbook PN A33116AB 23
Reviewing Detectos PDA Data Display 4.5 PDA Data Display Figue 4.9 Instument Window with PDA data file open; View menu open and PDA View selected Photo Diode Aay data featues PDA View 24 32 Kaat 8.0 PN A33116AB
Reviewing Detectos PDA Data Display 4 View Options Figue 4.10 Photo Diode Aay data P/ACE MDQ Taining Wokbook PN A33116AB 25
Reviewing Detectos LIF Detecto 4.6 LIF Detecto LIF Initial Conditions Figue 4.11 LIF Initial Conditions tab Electopheogam Channels Dynamic ange Filte Settings Peak width Signal Lase/Filte Desciptions Excitation wavelength Emission wavelength 26 32 Kaat 8.0 PN A33116AB
Reviewing Detectos Data Rate 4 4.7 Data Rate Relays On/Off LIF Data Display Figue 4.12 Instument Window with LIF data file open; ight mouse click menu open P/ACE MDQ Taining Wokbook PN A33116AB 27
Reviewing Detectos Data Rate LIF Calibation Wizad Accessing LIF Calibation Wizad Figue 4.13 Instument Configuation dialog Figue 4.14 LIF Detecto Calibation Wizad - Step 1 28 32 Kaat 8.0 PN A33116AB
Reviewing Detectos Summay 4 Figue 4.15 LIF Detecto Calibation Wizad - Step 2 Figue 4.16 LIF Detecto Calibation Wizad - Step 3 4.8 Summay This completes the detecto potion of the 32 Kaat Softwae Basic Instument Taining. You ae eady to use the Diect Contol featue. P/ACE MDQ Taining Wokbook PN A33116AB 29
Reviewing Detectos Summay 30 32 Kaat 8.0 PN A33116AB
Using Diect Contol Oveview 5 5Using Diect Contol 5.1 Oveview This section coves the stat up pocess and paamete contol fo P/ACE MDQ system. You will establish initial unning conditions fo each component. We will discuss: Accessing Diect Contol Diect Contol sceens Contolling module paametes Skill Check Accessing Diect Contol Figue 5.1 Instument Window with Contol Diect Contol selected P/ACE MDQ Taining Wokbook PN A33116AB 31
Using Diect Contol Using Diect Contol 5.2 Using Diect Contol Figue 5.2 Diect Contol Sceen Catidge Coolant Tempeatue B Set Tempeatue Sample Stoage Tempeatue B Set Tempeatue Capillay Infomation B Capillay Desciption B Capillay Lot Numbe 32 32 Kaat 8.0 PN A33116AB
Using Diect Contol Using Diect Contol 5 Rinse Inject Figue 5.3 Rinse dialog Figue 5.4 Inject dialog P/ACE MDQ Taining Wokbook PN A33116AB 33
Using Diect Contol Using Diect Contol Sepaate Figue 5.5 Sepaate dialog Detecto Settings Common Paametes Data Filtes Peak Width Relays Detection Signal Data Acquisition UV Detecto Paametes Wavelength selection Data Rate Filte Positions PDA Detecto Paametes Channel data Scan Data 34 32 Kaat 8.0 PN A33116AB
Using Diect Contol Using Diect Contol 5 Refeence Channel Shutte LIF Detecto Paametes Electopheogam Channels 1 and 2 Lamp Status Lase Status Tay Position Dynamic Range Data Rate Lase / Filte Desciption On/Off On/Off Gaphical display Voltage Settings Voltage Duation Ramp Time Voltage Max Cuent Max Tay Positions Extenal Adapte Polaity Pessue Cuent Settings Cuent Duation P/ACE MDQ Taining Wokbook PN A33116AB 35
Using Diect Contol Using Diect Contol Powe Settings Ramp Time Voltage Max Cuent Max Tay Positions Extenal Adapte Polaity Pessue Powe Duation Ramp Time Voltage Max Cuent Max Tay Positions Extenal Adapte Polaity Pessue Pessue Pessue / Vacuum Duation Tay Positions Diection Pessue Type Time Remaining Gaphical display 36 32 Kaat 8.0 PN A33116AB
Using Diect Contol Using Diect Contol 5 Status Figue 5.6 Instument Window with Contol Instument Status View selected P/ACE MDQ Taining Wokbook PN A33116AB 37
Using Diect Contol Skill Check Figue 5.7 Status Window Status Item Cuent Status Action 5.3 Skill Check Upon completion of this section, you should be able to do the following: Access Diect Contol. Pogam and un a inse. Pogam and un an injection. Pogam and un a sepaation. Summay This completes the Diect Contol potion of 32 Kaat Softwae Basic Instument Taining. When the system finishes equilibating, you will pepae a method to un. 38 32 Kaat 8.0 PN A33116AB
Woking with Methods Oveview 6 6Woking with Methods 6.1 Oveview In this section we will explain pocedues fo building and editing a method. A method automates all of the hadwae functions as well as data collection. Late we will talk about editing, analysis paametes, and epot geneation). In this section, we will discuss: Method Wizad Ceating a Method Saving a Method Editing a Method Pinting a Method Othe Method Popeties Skill Check Using the Method Wizad Figue 6.1 Method Window dialog B Ceating a new method B Modifying the cuent method B Modifying a method on disk P/ACE MDQ Taining Wokbook PN A33116AB 39
Woking with Methods Oveview Ceating a Method Figue 6.2 Instument Window with File Method New selected Figue 6.3 Instument Window with Method Instument Setup selected 40 32 Kaat 8.0 PN A33116AB
Woking with Methods Oveview 6 Instument Setup Figue 6.4 Instument Setup Window with Time Pogam tab P/ACE MDQ Taining Wokbook PN A33116AB 41
Woking with Methods Oveview Initial Conditions Tabs Figue 6.5 Initial Conditions tab Auxiliay data channels Mobility Channels Tempeatue Peak detect paametes Tigge settings Tay type selection Analog output scaling 42 32 Kaat 8.0 PN A33116AB
Woking with Methods Oveview 6 Time Pogamming Time Pogamming tab Time Event Value Duation Inlet Vial/Outlet Vial Summay Comments P/ACE MDQ Taining Wokbook PN A33116AB 43
Woking with Methods Oveview Figue 6.6 Time Pogam tab with available Event list displayed Events Rinse Inject Sepaate UV Detecto PDA Detecto LIF Detecto Associated Event dialog boxes Associated Event dialog boxes Associated Event dialog boxes Extenal Detecto Selecting an Extenal Detecto Adapte 44 32 Kaat 8.0 PN A33116AB
Woking with Methods Oveview 6 Saving a Method Figue 6.7 Instument Window with File Method Save selected Save Method As Save As Default Figue 6.8 Save icon with Method selected Naming and saving a new method Saving an existing method P/ACE MDQ Taining Wokbook PN A33116AB 45
Woking with Methods Oveview Editing a Method Method Wizad Open method Figue 6.9 Open Method File dialog Make changes Save Method o Save Method As 46 32 Kaat 8.0 PN A33116AB
Woking with Methods Oveview 6 Pinting a Method Figue 6.10 Instument Window with File Method Pint selected Othe Method Functions Popeties Figue 6.11 Desciption tab Method Desciption P/ACE MDQ Taining Wokbook PN A33116AB 47
Woking with Methods Oveview Figue 6.12 Options tab Analysis Data Compession Figue 6.13 Calibation tab Automatically Aveage Replicates Response Facto Definition Peak Identification Options 48 32 Kaat 8.0 PN A33116AB
Woking with Methods Oveview 6 Figue 6.14 Audit Tail tab Audit Tail P/ACE MDQ Taining Wokbook PN A33116AB 49
Woking with Methods Skill Check 6.2 Skill Check Upon completion of this section, you should be able to do the following: 1. Ceate a new method fo a constant voltage sepaation in a 60 cm, 75 µm I.D. capillay. 2. Pepae the following 2 ml vials fo inlet and outlet vial positions and fo inses: Vial Contents Volume Position Rinse Run Buffe A 2 ml BI:B1 Injection Run Buffe A 1 ml BO:B1 Sepaation Run Buffe A 2 ml BI:A1 Sepaation Run Buffe A 2 ml BO:A1 Waste N/A 0 BO:A3 S = Sample; B = Buffe I = Inlet; O = Outlet A1 though F6 indicate Vial Positions 3. Fo UV and PDA detectos, fill one 2 ml vial with Beckman Coulte Test Mix B and place in the SI:A1 position. If you ae using an LIF detecto, fill the 2 ml vial with Beckman Coulte LIF Detecto Test Mix. (Pepae LIF Detecto Test Mix accoding to the Test Mix diections.) 4. Set the initial tempeatue to 23 C. 5. Pogam an un-timed pe-inse (with a duation of at least two minutes at 20-30 psi) of Run Buffe A (BI:B1) to the waste vial (BO:A3). 6. Pogam a 10 second fowad pessue injection of Beckman Coulte Test Mix (SI:A1) at 0.5 psi to the injection buffe vial (BO:B1). 7. Pogam a 30.0 kv constant voltage sepaation using a 0.17 minute amp time and a duation of 6.0 minutes. Select the At Time check box and ente 0.00 minutes. 8. If you ae using a UV detecto, set the wavelength to 214 nm. If you ae using a PDA, set the wavelength to 214 nm with a 10 nm bandwidth. If you ae using an LIF detecto make sue that the appopiate filtes ae in place. 9. Save the method you have ceated as TestMixB.met. Summay This completes the methods development potion of the 32 Kaat Softwae Basic Instument Taining. We ae eady to un an actual sepaation. 50 32 Kaat 8.0 PN A33116AB
Running A Sample Oveview 7 7Running A Sample 7.1 Oveview We will now use the method you pepaed in the pevious lesson to geneate an electopheogam of Beckman Coulte Test Mix. We will discuss: Running a Single Sample Stopping / Aboting Method Data Display Skill Check 7.2 Running a Single Sample Figue 7.1 Single Run Acquisition dialog Sample ID Method Data path Data file P/ACE MDQ Taining Wokbook PN A33116AB 51
Running A Sample Stopping / Aboting Method Desciption Figue 7.2 Sample Desciption dialog Stat 7.3 Stopping / Aboting Method Stop Run Abot Run 52 32 Kaat 8.0 PN A33116AB
Running A Sample Displaying Data 7 7.4 Displaying Data Figue 7.3 Instument Window with TestMixB.dat open P/ACE MDQ Taining Wokbook PN A33116AB 53
Running A Sample Displaying Data Figue 7.4 Instument Window with data file open and ight mouse click menu open Add Tace Figue 7.5 New Tace Popeties dialog 54 32 Kaat 8.0 PN A33116AB
Running A Sample Displaying Data 7 New Tace Annotation Appeaance Add Multiple Taces Figue 7.6 Open Data File dialog P/ACE MDQ Taining Wokbook PN A33116AB 55
Running A Sample Displaying Data Axis Setup Figue 7.7 Axis Popeties Setup dialog Axis Setup Gaph Title Autoscale Range Magins Geneal Options Oientation 56 32 Kaat 8.0 PN A33116AB
Running A Sample Displaying Data 7 Annotations Figue 7.8 Tace Annotation Popeties dialog Taces Available Annotations list box Othe options Appeaance Figue 7.9 Appeaance Popeties dialog P/ACE MDQ Taining Wokbook PN A33116AB 57
Running A Sample Displaying Data Scheme Line Style Item Font Full Unzoom Opeations Figue 7.10 Opeations Sub-menu 58 32 Kaat 8.0 PN A33116AB
Running A Sample Displaying Data 7 Utilities Figue 7.11 Utilities Sub-menu Popeties Figue 7.12 Data Gaph Popeties dialog P/ACE MDQ Taining Wokbook PN A33116AB 59
Running A Sample Skill Check 7.5 Skill Check Upon completion of this section, you should be able to do the following: 1. Run the method TestMixB.met. 2. Zoom the display scale. 3. Auto zeo fom the status window. 4. Change the selections in the status window. 5. Save the data as TestMixB.dat. Summay Congatulations! You have just geneated you fist electopheogam with you new P/ACE MDQ system. Next we will lean how to analyze the data. 60 32 Kaat 8.0 PN A33116AB
Analyzing and Integating Data Oveview 8 8Analyzing and Integating Data 8.1 Oveview Now that we have collected some actual un data, we will use the integation featues of 32 Kaat Softwae to begin analysis of the sample. We will discuss: Opening data files Gaphical integation events pogamming Defining and Naming Peaks Identifying Peaks based on Migation Time Identifying Peaks based on Mobility Annotation of the on-sceen Display Skill Check 8.2 Opening Data Files Figue 8.1 Instument Window with data file open and Analysis menu selected P/ACE MDQ Taining Wokbook PN A33116AB 61
Analyzing and Integating Data Opening Data Files Figue 8.2 Analyze icon Gaphical Pogamming Figue 8.3 Gaphical Pogamming Sub-menu B Define Peaks 62 32 Kaat 8.0 PN A33116AB
Analyzing and Integating Data Opening Data Files 8 Analyzed electopheogam Figue 8.4 Instument Window with an analyzed data file open P/ACE MDQ Taining Wokbook PN A33116AB 63
Analyzing and Integating Data Optimizing Integation 8.3 Optimizing Integation The Integation Tool ba is located at the bottom of the Instument window o, fom the ight mouse button-click menu and select Gaphical Pogamming. Figue 8.5 Integation Events Toolba Figue 8.6 Instument Window with ight mouse click menu open and Gaphical Pogamming selected Set Width Set Theshold Integation Off 64 32 Kaat 8.0 PN A33116AB
Analyzing and Integating Data Optimizing Integation 8 Manual Integation Fixes vs. Integation Events Table Valley to Valley Hoizontal Baseline Backwad Hoizontal Baseline Tangent Skim Font Tangent Skim Minimum Aea Negative Peak Disable Peak End Detection Reassign Peak Manual Baseline Manual Peak Split Peak Foce Peak Stat Foce Peak Stop Move Baseline Stat Move Baseline Stop Pecentile Point Left Slope Sensitivity Right Slope Sensitivity Define Single Peak Define Peaks Define Goups P/ACE MDQ Taining Wokbook PN A33116AB 65
Analyzing and Integating Data Defining and Naming Peaks Suggest Sampling Fequency 8.4 Defining and Naming Peaks Figue 8.7 Instument Window with Define Peaks selected Define Peaks Figue 8.8 Define Peaks icon Select Stat of Named Peak Range Select End of Named Peak Range 66 32 Kaat 8.0 PN A33116AB
Analyzing and Integating Data Defining and Naming Peaks 8 Figue 8.9 Define Peaks dialog Define peaks in ange Retention time window Units Quantitate on Aea o Height Identifying Peaks based on Migation Time Figue 8.10 Method Popeties dialog Defining Peaks Viewing Peak ID Table P/ACE MDQ Taining Wokbook PN A33116AB 67
Analyzing and Integating Data Defining and Naming Peaks Figue 8.11 Peak ID Table Ente peak names in Peak ID Table 68 32 Kaat 8.0 PN A33116AB
Analyzing and Integating Data Defining and Naming Peaks 8 Figue 8.12 Instument Window with the ight mouse click menu and Annotations highlighted P/ACE MDQ Taining Wokbook PN A33116AB 69
Analyzing and Integating Data Defining and Naming Peaks Figue 8.13 Tace Annotation Popeties dialog Annotation of on-sceen display Figue 8.14 Data Display with Annotations fo Time-Based Peak Identification 70 32 Kaat 8.0 PN A33116AB
Analyzing and Integating Data Defining and Naming Peaks 8 Identifying Peaks based on Mobility Figue 8.15 Method Popeties dialog Defining Peaks Figue 8.16 Peak ID Table with Mobility Make selected P/ACE MDQ Taining Wokbook PN A33116AB 71
Analyzing and Integating Data Defining and Naming Peaks Figue 8.17 Tace Annotations Popeties dialog Figue 8.18 Data Display with Annotations fo Mobility - Based Peak identification 72 32 Kaat 8.0 PN A33116AB
Analyzing and Integating Data Skill Check 8 8.5 Skill Check Upon completion of this section, you should be able to do the following: 1. Open the Test Mix B.dat data file. 2. Afte data collection, add a Peak ID Table to identify the two peaks. 3. Analyze the data. 4. Set the peak width and theshold to obtain acceptable integation of both peaks. 5. Build a table to name the peaks. (The elution ode is Benzoic acid and then Phenyl acetic acid.) 6. Make sue Time based is selected in the Options tab of the Method Popeties dialog. 7. Set the paametes in the Peak ID table to identify peaks based on migation time. 8. Analyze the data and apply annotations to show names, migation times and coected aeas on the on-sceen electopheogam display. 9. Change the selection in the Options tab to Mobility based. 10. Change the paametes in the Peak ID table to identify peaks based on mobility. 11. Reanalyze the data. 12. Apply the appopiate annotations to the data display. Summay This completes the Analysis and Integation potion of the Basic Instument Taining. Be sue to come back and exploe all the vaious integation and peak identification options to find the ones that best suit you own wok. Next we will automate goups of consecutive uns using Sequence Tables. P/ACE MDQ Taining Wokbook PN A33116AB 73
Analyzing and Integating Data Skill Check 74 32 Kaat 8.0 PN A33116AB
Using Sequence Tables Oveview 9 9Using Sequence Tables 9.1 Oveview In this section we will futhe automate the system by defining a sequence of methods and samples to un unattended. This is accomplished using a Sequence Table. We will discuss: Sequence Wizad Editing Sequence Tables Saving Sequence Tables Running Sequence Tables 9.2 Using the Sequence Wizad Figue 9.1 Instument Window with File Sequence New selected P/ACE MDQ Taining Wokbook PN A33116AB 75
Using Sequence Tables Using the Sequence Wizad Figue 9.2 Sequence Wizad - Methods dialog Method Infomation Data File Type Amount values Figue 9.3 Sequence Wizad - Unknowns dialog Infomation about unknown and data file 76 32 Kaat 8.0 PN A33116AB
Using Sequence Tables 9 Using the Sequence Wizad Figue 9.4 Sequence Wizad - Vials dialog Infomation about vials Figue 9.5 Sequence Wizad - Calibation dialog Calibation Infomation P/ACE MDQ Taining Wokbook PN A33116AB 77
Using Sequence Tables Using the Sequence Wizad Figue 9.6 Sequence Wizad - Repots dialog Repots Infomation Summay System Suitability QC Check Standad 78 32 Kaat 8.0 PN A33116AB
Using Sequence Tables Viewing a Sequence 9 9.3 Viewing a Sequence Figue 9.7 Instument Window with File Sequence Open selected Figue 9.8 Sequence Table Status Run Type P/ACE MDQ Taining Wokbook PN A33116AB 79
Using Sequence Tables Viewing a Sequence Clea All Calibation Begin Summay Clea Calibation at Level Summay Run Pint Calibation Repot End Summay Aveage Replicates Vial Summay Clea Replicates QC Check Standad Begin Loop Unspiked End Loop Spiked Shutdown Spike 1 of 2 Pint Additional Repots Spike 2 of 2 Begin System Suitability Duplicate System Suitability Standad Begin Calibation End System Suitability End Calibation Level Concentation Oveide Repetitions Sample ID Method Filename Sample Amount ISTD Amount Multiplie Action Desciption 80 32 Kaat 8.0 PN A33116AB
Using Sequence Tables Editing a Sequence 9 9.4 Editing a Sequence Figue 9.9 Instument Window with Sequence Edit selected Figue 9.10 Edit Sequence icon P/ACE MDQ Taining Wokbook PN A33116AB 81
Using Sequence Tables Saving a Sequence 9.5 Saving a Sequence Figue 9.11 Saving Sequence dialog Save Sequence Save Sequence As 82 32 Kaat 8.0 PN A33116AB
Using Sequence Tables Running a Sequence 9 9.6 Running a Sequence Figue 9.12 Instument Window with Contol Sequence Run selected Figue 9.13 Sequence Run icon P/ACE MDQ Taining Wokbook PN A33116AB 83
Using Sequence Tables Running a Sequence Figue 9.14 Run Sequence dialog Figue 9.15 Instument Window with Sequence Pocess selected Pocess 84 32 Kaat 8.0 PN A33116AB
Using Sequence Tables Running a Sequence 9 Figue 9.16 Pocess Sequence dialog Sequence Infomation Run Range Mode Review Pinting P/ACE MDQ Taining Wokbook PN A33116AB 85
Using Sequence Tables Skill Check 9.7 Skill Check Upon completion of this section, you should be able to do the following: 1. Run the same method (TestMixB.met) thee times. 2. Specify sample vial SI:A1 as the sample fo line 1, vial SI:F10 as the sample fo line 2 and vial SI:C8 as the sample fo line 3. 3. Specify sequential file names fo the data. 4. Do NOT un the sequence table at this time. Summay This completes the Sequence potion of the 32 Kaat Softwae Basic Instument Taining. We can now automate unning and pocessing the data of multiple samples. In the next section we will take a look at the steps necessay to automatically geneate and apply calibation data. 86 32 Kaat 8.0 PN A33116AB
Ceating Calibations Oveview 1 10Ceating Calibations 10.1 Oveview The Peak ID and Sequence Tables have enty columns that we have not yet discussed. These paametes ae used fo geneating and updating the calibation data fo a given method. To geneate a calibation cuve we will: Edit a Peak ID Table fo calibation Ceate a Calibation Sequence Table Run a Single Level Calibation Sequence Review Calibation Cuves Final Skill Check 10.2 Editing the Peak ID Table Figue 10.1 Instument Window with Method Peaks Goups selected P/ACE MDQ Taining Wokbook PN A33116AB 87
Ceating Calibations Editing the Peak ID Table Figue 10.2 Peak ID Table set fo Calibation Popeties - Select paametes Paamete Options Name Weighting Method ID 10 Levels Ref ID # STD ID # ISTD ID # STD Mult. Units Low Conc Analysis Channel High Conc Quantitate Check Std 1 Conc Fit Type Check Std 1%RD Zeo Spike 1 Amount Calib Flag Spike 2 Amount Calib Weight Dup %RD Limit % Calib Magin RF %RSD Limit Scale 88 32 Kaat 8.0 PN A33116AB
Ceating Calibations 1 Ceating a Calibation Sequence with the Sequence Wizad 10.3 Ceating a Calibation Sequence with the Sequence Wizad Figue 10.3 Instument Window with File Sequence New selected Figue 10.4 Sequence Wizad - Calibation dialog Calibation ID Calibation Path P/ACE MDQ Taining Wokbook PN A33116AB 89
Ceating Calibations Ceating a Calibation Sequence with the Sequence Wizad Calibation file Numbe of levels Numbe of Repetitions pe level Figue 10.5 Completed Sequence 90 32 Kaat 8.0 PN A33116AB
Ceating Calibations 1 Running a Calibation Sequence 10.4 Running a Calibation Sequence Figue 10.6 Instument Window with Contol Sequence Run selected Figue 10.7 Run Sequence icon Figue 10.8 Run Sequence dialog P/ACE MDQ Taining Wokbook PN A33116AB 91
Ceating Calibations Reviewing Calibation Cuves 10.5 Reviewing Calibation Cuves Figue 10.9 Instument Window with Method Review Calibation selected Figue 10.10 Review Peak Calibation icon 92 32 Kaat 8.0 PN A33116AB
Ceating Calibations 1 Reviewing Calibation Cuves Figue 10.11 Review Peak Calibation Window Selecting a cuve to eview Deleting data points Equation and goodness of fit P/ACE MDQ Taining Wokbook PN A33116AB 93
Ceating Calibations Final Skill Check 10.6 Final Skill Check Upon completion of this section, you should be able to do the following: 1. Edit the peak ID table in you method to specify thee calibation levels. (Five level calibation is shown in this section.) 2. Modify the sequence table you ceated in the pevious section to specify the un types as calibation (ente the level numbe in the Level column to change un type fom Unknown to Calibation). 3. Dilute the test mix to ceate thee levels of concentations fo calibation. 4. Specify sequential file names fo the calibation standads. 5. Use Diect Contol to bing the tays to the load position. Veify that the Test Mix vials ae in the appopiate positions. 6. Run the Sequence table. 7. When the un is complete, eview you calibation data. Summay You now know the basics of geneating, analyzing and epoting electophoetic data. Next we will set up customized epots and sequence epots. 94 32 Kaat 8.0 PN A33116AB
Pepaing Custom Repots Oveview 1 11Pepaing Custom Repots 11.1 Oveview This section consides the ceation of custom epots. We will discuss: Accessing a Custom Repot Editing a Custom Repot Ceating a Repot Skill Check 11.2 Accessing and Editing a Custom Method Repot Figue 11.1 Instument Window with Method Custom Repot selected P/ACE MDQ Taining Wokbook PN A33116AB 95
Pepaing Custom Repots Accessing and Editing a Custom Method Repot Figue 11.2 Instument Window with File Repot Template selected Figue 11.3 Repot Template Open dialog 96 32 Kaat 8.0 PN A33116AB
Pepaing Custom Repots 1 Accessing and Editing a Custom Method Repot Figue 11.4 Method Repot Template with ight-click menu displayed P/ACE MDQ Taining Wokbook PN A33116AB 97
Pepaing Custom Repots Ceating a Custom Method Repot 11.3 Ceating a Custom Method Repot Figue 11.5 Instument Window - Custom Repot, ight-click menu displayed 98 32 Kaat 8.0 PN A33116AB
Pepaing Custom Repots 1 Ceating a Custom Method Repot Instument Window - Custom Repot, ight-click menu, Inset Field selected Inseting a Field P/ACE MDQ Taining Wokbook PN A33116AB 99
Pepaing Custom Repots Ceating a Custom Method Repot Figue 11.6 Instument Window - Custom Repot; ight-click menu, Inset Gaph selected Inseting a Gaph 100 32 Kaat 8.0 PN A33116AB
Pepaing Custom Repots 1 Ceating a Custom Method Repot Figue 11.7 Instument Window - Custom Repot; ight-click menu, Inset Repot Selected Inseting a epot Fomat columns P/ACE MDQ Taining Wokbook PN A33116AB 101
Pepaing Custom Repots Ceating a Custom Method Repot Run Repot dialog Figue 11.8 Fomatted Custom Repot 102 32 Kaat 8.0 PN A33116AB
Pepaing Custom Repots Skill Check 1 11.4 Skill Check Upon completion of this section, you should be able to do the following: 1. Stat and configue 32 Kaat Softwae. 2. Establish initial conditions to un the method used to geneate you fist electopheogam. 3. Run a sample of test mix. 4. Analyze the data and geneate a custom epot that includes: A heade and foote Infomation fields An electopheogam with identified peaks (data gaph) A un epot 5. Save the epot as a template and pint the final epot. Summay Congatulations! You have successfully completed 32 Kaat Softwae Basic Instument Taining. We suggest that you epeat these execises on you own befoe beginning wok with you own methods and samples. P/ACE MDQ Taining Wokbook PN A33116AB 103
Pepaing Custom Repots Skill Check 104 32 Kaat 8.0 PN A33116AB
Summay 1 Advancing You Skill 12Summay 12.1 Advancing You Skill We sinceely hope that you ae pleased with you new P/ACE MDQ system. 32 Kaat Softwae has many additional featues and functions that ae beyond the scope of this intoduction. These include: System Administation System Suitability Data Expot Use-defined data manipulation To help you benefit fom these advanced featues, we have povided you with Online Help. This Help is also available as an on-line manual which can be found on the 32 Kaat Softwae Manual CD-Rom. Please take some time to familiaize youself with these efeences. If you would like to enoll in one of ou advanced seminas o puchase additional in-lab taining, please contact you local sevice epesentative. P/ACE MDQ Taining Wokbook PN A33116AB 105
Summay Recod of Opeato Taining 12.2 Recod of Opeato Taining Taining Summay The following pages list the contents of 32 Kaat Softwae Basic Taining Wokbook. Items initialed by the instucto ae elevant to the system tained and wee coveed. Items maked with an x ae not elevant to the system tained and wee not coveed. Instucto Name Opeato Name Opeato Name 106 32 Kaat 8.0 PN A33116AB
Summay 1 Recod of Opeato Taining Checklist: System Oveview Optimizing Integation Hadwae Teminology Defining and Naming Peaks Safety Featues Identifying Peaks using Migation Safety Notices Identifying Peaks using Mobility Chemical/Biological Safety UV, PDA o LIF Initial Conditions Electical Safety PDA Setup Electostatic Dischage UV, PDA o LIF Data Display Windows Exploe LIF Calibation Wizad Accessing 32 Kaat Softwae Using the Sequence Wizad Configuing 32 Kaat Softwae Viewing a Sequence Stating Newly Configued Editing Sequences Instument Accessing Diect Contol Saving Sequences Using Diect Contol Running Sequences Using the Method Wizad Accessing Custom Repots Ceating a Method Ceating Custom Repots Saving a Method Editing Peak ID Table fo Editing a Method Calibation Pinting a Method Ceating Calibation Othe Method Functions Sequences with Sequence Wizad Running a Single Sample Running Calibation Sequences Stopping / Aboting Methods Reviewing Calibation Cuves Displaying Data Opening Data Files P/ACE MDQ Taining Wokbook PN A33116AB 107
Summay Recod of Opeato Taining Signatues: Opeato Date Opeato Date The peson(s) listed above have eceived basic instuction fom the epesentative signed below. Instucto Date 108 32 Kaat 8.0 PN A33116AB