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UMDNT SCHOOLOF 0 STEOPATH IC Page 1 of2 University of Medicine & Dentistry of New Jersey Office of Research and Sponsored Programs June 4,2007 Division of Nuclear Materials Safety US Nuclear Regulatory Commission Region I Office 475 Allendale Road King of Prussia, PA 19406-14 15 ATTENTION: Licensing Assistance Team RE: Addition of Authorized User License No. 29-20876-01; Docket No. 030-2 1288 Dear Sir/Madam: Please add Hristo B. Houbaviy, Ph.D. Assistant Professor, Department of Cell Biology, to the list of approved users (Item 11) for Items 6.A. through 6.1. As shown in the attached table, Professor Houbaviy has extensive experience in handling tracer levels of radioactive materials. He will attend the UMDNJ-SOM training sessions to refresh his formal training and to acquaint him with the institutional procedures and practices. Please delete the names of R. Sharma, Ph.D., and T. Duda, Ph.D., from the approved user list. They left the UMDNJ-SOM. If you need additional information please contact me at 609 9 19-0275 or esc8@,comcast.net). Edward A. Christman, Ph.D., CHP Radiation Safety Officer Cc: JSY 22.. -.L NMSSIRGNl MATERIALS-002 40 East Laurel Road, Suite 1040, Stratford, NJ 08084 Phone: 856-566-607516066 Fax: 856-566-6299 0 som.umdnj.edu/research The University is an Affirmative Action/Equal Opportunity Employer

Statement of Prior Experience with Radioactive Materials and Radiation Sources Hristo B. Houbaviy, Ph. D. From 1990 to 1993 I was an MSc student at the Weizmann Institute of Science, Rehovot, Israel. During that period I acquired substantial experience with the handling of radioactive materials. Experiments involved the routine handling of 0.5 mci 32P labeled material er week as well as smaller amounts of 35S labeled material. I also performed l2{ labeling of proteins. The Weizmann Institute provided standard radiation safety training to all personnel working with radioactive materials. From 1993 to 1999 I was a Ph. D. student at the Rockefeller University, New York, New York, USA. During this period I continued using 32P in my research and had to pass the radiation safety requirements of the Rockefeller University. in addition, I performed experiments at the National Synchrotron Light Source (NSLS) of the Brookhaven National Laboratory (BNL), Brookhaven, New York, USA as well as at the Cornell High Energy Synchrotron Source (CHESS), Ithaka, New York, USA. Both synchrotron facilities provided appropriate radiation safety training. At the Brookhaven Natonal Laboratory I passed the Department of Energy General Radiation Training (GERT) certification. From 1999 to 2005 I was a postdoctoral associate at the Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA. During that period I worked mostly with 32P. MIT provided the appropriate radiation safety training. From 2005 to 2007 I was a research assistant professor at Rutgers University, Piscataway, New Jersey. During this period I worked mostly with 32P. Rutgers University provided the appropriate radiation safety training.

, Curriculum Vitae of Hristo Botev Houbaviy, Ph. D. Address: Department of Genetics Rutgers University 145 Bevier Road Piscataway, NJ 08854-8009 Telephone: (732) 445-1027 x40033 Facsimile: (732) 445-1147 E-mail: houbaviy@biology.rutgers.edu Born: Citizenship: Immigration Status: Positions: April, 2007 - present: Assistant Professor, Department of Cell Biology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Stratford, NJ, USA October, 2005-April, 2007: Research Assistant Professor, Department of Genetics, Rutgers University, Piscataway, NJ, USA July, 1999-September, 2005: Education: Postdoctoral Associate (July, 2000-June 2002, Postdoctoral Fellow), Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA Supervisor: Professor Phillip A. Sharp. September, 1993-June, 1999: Ph. D. (Molecular Biophysics), Laboratories of Molecular Biophysics, The Rockefeller University, New York, NY, USA. Thesis supervisor: Professor Stephen K. Burley. Thesis title: Biochemical and biophysical studies of the complex formed by YY 1 and the AAV P5 promoter initiator element. PERSONAL INFORMATION WAS REMOVED BY NRC. NO COPY OF THIS INFORMATION WAS RETAINED BY THE NRC. Curriculum Vitae Page 1

September, 1990-August, 1993: M. Sc. (Life Sciences) Department of Molecular Genetics and Virology and The Feinberg Graduate School, The Weizmann Institute of Science, Rehovot, Israel. Thesis supervisor: Professor Yosef Aloni. Thesis title: Specific interaction between the carboxy-terminal domain of RNA polymerase I1 and the TATA-box binding protein: an in vitro study. September, 1986-August, 1990: Awards: B. Sc. (Biotechnology) Faculty of Biology, The St. Kliment Ohridski University of Sofia, Sofia, Bulgaria. Diploma student (September, 1988-August, 1990), Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria. Postdoctoral fellowship from the Jane Coffin Childs Memorial Fund for Medical Research (July, 2000 - June, 2002). NSF travel scholarship to attend Keystone Symposium, Emerging Mechanisms of Epigenetic Regulation, Tahoe City, CA (January 2004). Travel scholarship to attend Keystone Symposium, sirnas and mirnas, Keystone, CO (April 2004). Stem Cell Research Grant from the New Jersey Commission on Science and Technology (January 2006 - December 2007). Project title: MicroRNAs mir-290-295 in Blastocyst- Derived Stem Cells and the Early Mouse Embryo. Total amount of funding: $300, 000. Invited presentations: An Embryonic Stem Cell-Specific microrna Cluster, presentation at a plenary session of Keystone Symposium, Emerging Mechanisms of Epigenetic Regulation, Tahoe City, CA (January 2004). Publications: 1. Usheva, A., Maldonado, E., Goldring, A., Lu, H., Houbavi, C., Reinberg, D. and Aloni, Y., Specific interaction between the nonphosphorylated form of RNA polymerase I1 and the TATA-binding protein., Cell, 69: 871-881 (1992) 2. Houbaviy, H. B., Usheva, A., Shenk, T. and Burley, S.K., Co-crystal structure of YY1 bound to the adeno-associated virus P5 initiator., Proc. Natl. Acad. Sci. USA, 93: 13577-13582 (1996) Curriculum Vitae Page 2

3. Houbaviy, H. B. and Burley, S. K., Thermodynamic analysis of the interaction between YY1 and the AAV P5 promoter initiator element. Chemistry & Biology, 8: 179-187 (2001) 4. Houbaviy, H. B., Murray, M. F. and Sharp, P. A., Embryonic stem cell-specific micrornas, Developmental Cell, 5: 351-358 (2003) (A "News and Views" report about this paper appeared in Nature, 424:898 (2003)) 5. Houbaviy, H. B., et al., Characterization of a highly variable eutherian microrna gene, RNA, 11:1245-1257 (2005) 6. Houbaviy, H. B., micrornas in the stem cells of the blastocyst. In MicroRNAs: from basic science to disease biology, Appasani, K., ed. (Cambridge University Press, Cambridge, UK), in the press. References: Phillip A. Sharp, Ph. D. Institute Professor Center for Cancer Research and Department of Biology Massachusetts Institute of Technology 40 Ames Street Cambridge, MA 02 139-4307 Telephone: (617) 253-6421 Facsimile: (617) 253-3867 E-mail: sharppa@mit.edu Stephen K. Burley, M. D., D. Phil., F. R. S. C. Chief Scientific Officer and Senior Vice-president for Research Structural GenomiX, Inc. 10505 Roselle Street San Diego, CA 92 12 1 Telephone: (858) 558-4850, ext. 1263 Private Line: (858) 228-1463 Secure Facsimile: (858) 558-6079 E-mail: sburley@stromix.com Rudolf Jaenisch, M. D. Professor of Biology, Massachusetts Institute of Technology Member, The Whitehead Institute for Biomedical Research Nine Cambridge Center Cambridge, MA 02 142-1479 Telephone: (617) 258-5186 Facsimile: (617) 258-6505 E-mail: jaenisch@wi.mit.edu Curriculum Vitae Page 3

David P. Bartel, Ph. D. Associate Professor of Biology, Massachusetts Institute of Technology Member, The Whitehead Institute for Biomedical Research Nine Cambridge Center Cambridge, MA 02 142-1479 Telephone: (617) 258-5287 Facsimile: (617) 258-6768 E-mail: dbartel@wi.mit.edu Curriculum Vitae Page 4

This is to acknowledge the receipt of your letter/application dated, and to inform you that the initial processing which includes an administrative review has been performed. a&#. %9-Wg76-0/ &There were no administrative omissions. Your application was assigned to a technical reviewer. Please note that the technical review may identify additional omissions or require additional information. Please provide to this office within 30 days of your receipt of this card A copy of your action has been forwarded to our License Fee 8, Accounts Receivable Branch, who will contact you separately if there is a fee issue involved. Your action has been assigned Mail Control Number /pd* 7. When calling to inquire about this action, please refer to this control number. You may call us on (610) 337-5398, or 337-5260. NRC FORM 532 (RI) @-W) Sincerely, Licensing Assistance Team Leader