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The online source of trusted techniques in molecular and cell biology Contains cutting-edge and classic protocols presented step-by-step with cautions and troubleshooting Frequently updated and annotated Interactive, customizable, and fully searchable Subject Coverage Antibodies Bioinformatics/Genomics Cell Biology Chromatography Computational Biology DNA Delivery/Gene Transfer Electrophoresis Emerging Model Organisms Genetics High-Throughput Analysis Imaging/Microscopy Immunology Laboratory Organisms Molecular Biology Neuroscience Newly Added Protocols Plant Biology Polymerase Chain Reaction (PCR) Proteins and Proteomics RNA Interference (RNAi)/siRNA Stem Cells Transgenic Technology C old Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Visit Cold Spring Harbor Protocols today and discover a rich, interactive source of new and classic research techniques. The site is fully searchable, with many tools that can be customized by users, including topic-based alerting and personal folders. Through a web-based editorial process, users also have the opportunity to add refereed comments to each protocol. Links in the online protocols offer additional resources and step-bystep instructions print out in a convenient form, complete with materials, cautions, and troubleshooting advice. Each protocol is citable, presented, and edited in the style that has made Molecular Cloning, Antibodies, Cells, and many other Cold Spring Harbor manuals essential to the work of scientists worldwide. The current collection of more than 1000 protocols is continuously expanded, updated, and annotated by the originators and users of the techniques. NEW to CSH Protocols: Emerging Model Organisms, a full-fledged guide to the use of new model systems in the laboratory, covering husbandry, genetics, genomics and basic protocols. CSH Protocols is created by Cold Spring Harbor Laboratory Press in association with HighWire Press of Stanford University. Executive Editor: Dr. David Crotty ISSN 1559-6095 / online, monthly Available exclusively via institutional site license For pricing information or to request a free trial, contact us at: Phone:1-800-843-4388 (Continental US and Canada) or 516-422-4100 (all other locations) Fax: 516-422-4097 E-mail: cshpress@cshl.edu Website: www.cshlpress.com Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924

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