DYMAPHY's Results DataBase Input/Output Data, Structure, and Web Access. ULCO/ LISIC-Team speaker: Pierre-Alexandre Hébert



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DYMAPHY's Results DataBase Input/Output Data, Structure, and Web Access ULCO/ LISIC-Team speaker: Pierre-Alexandre Hébert December 3, CCI, Boulogne-sur-Mer 1

DYMAPHY's Results DataBase Input/Output Data, Structure, and Web Access Overview A database: what for? Data overview Database structure Database implementation Database Web interface

A database: what for? Phytoplankton is central in DYMAPHY's project As an indicator of the quality of marine waters Many types of devices are available to detect, identify, count, or predict it Necessity to study, standardize and compare those different kinds of measures Many campaigns have been conducted In various: places, seasons, sampling frequencies, depths Either: statically (marine station) or dynamically (cruises) in discrete sample or in-line in fields or cultures

A database: what for? Necessity to gather those data into a database High quantity Many campaigns High frequency measures High heterogeneity Various measure devices Biological measures (Phytoplankton) + Physical & Chemical measures (Environmental factors) Extraction by various criteria Merging A public database, i.e. not restricted to DYMAPHY's partners To compare different ways to measure Phytoplankton In order to propose efficient measure protocols To improve signal processing and data analysis methods to count/identify Phytoplankton groups

Data Overview DYMAPHY's campaigns Inter-CALibrations exercises Cultures Monocultures (CAL-1, CAL-2, CAL-3) Mixtures (CAL-2 & CAL-3) Field samples Jacobahaven (Versemeere, Rompot) CAL-1 Lowestoft (estuarine-north Sea gradient) CAL-2 E. Channel (coastal-offshore gradient) CAL-3

Data Overview DYMAPHY's campaigns Common oceanographic cruises PROTOOL/DYMAPHY cruise R.V. «Endeavour» North Sea May 2011 DYPHYMA I cruise R.V. «Côtes de la Manche» Eastern Channel April 2012 «Dutch estuaries» cruise R.V. «Argus» Westerschelde, Oosterschelde and Grevelingen September 2012

Data Overview DYMAPHY's campaigns Local Monitoring/Observation networks English North Sea The Wash Smart Buoys Fisheries cruise North Sea monitoring cruise Dutch North Sea and estuaries S.E. North Sea monitoring Zyrphaea cruise Wetern/Eastern Schelde French North Sea and E. Channel Wimereux-Slack coastal-offshore gradient (LOG) DYPHYMA II cruise REPHY/SRN IFREMER estuarine-channel gradient (Somme Bay, Boulogne sur Mer, Dunkerque)

Data Overview - Field samples Dutch Estuaries CAL-1 common inter calibration Westerschelde/Oosterschelde monitoring «Dutch Estuaries» common cruise (R.V. «Argus» - RWS) English estuaries & coastal E. North Sea CAL-2 common inter calibration Wash Smart Buoy North Sea Protool/Dymaphy common cruise (R.V. «Endeavour» - Cefas) North Sea monitoring cruise (Cefas) Zyrphaea cruise (RWS) SE North Sea coastal monitoring (RWS) Southern North Sea (Dunkerque) coastal monitoring (SRN IFREMER) Eastern Channel CAL-3 common inter calibration Wimereux-Slack coastal-offshore gradient (LOG) Boulogne sur Mer and Bay of Somme coastal monitoring (SRN/REPHY IFREMER) DYPHYMA I common cruise across the E. Channel («R.V. «Côtes de la Manche» - INSU/CNRS) DYPHYMA II cruise across the E. Channel («R.V. «Côtes de la Manche» - INSU/CNRS)

Data Overview Various measure devices Flow Cytometry CytoSense (from Cytobuoys) Spectral Fluorometry BBE Fluoroprobe, PhytoPAM, Algae Online Analyser (AOA) Pigments measures HPLC Microscopy Physical & chemical measures Pocket Ferry Box (Temperature, salinity, O2, pressure...) Fluorescence extraction CTD sonde (YSI) Technicon Alliance TOC analyser (Dissolved Organic Carbon)

Data Overview Various measure devices Flow Cytometry CytoSense (from Cytobuoys) Spectral Fluorometry BBE Fluoroprobe, PhytoPAM, Algae Online Analyser (AOA) Pigments measures HPLC Microscopy Physical & chemical measures Pocket Ferry Box (Temperature, salinity, O2, pressure...) Fluorescence extraction CTD sonde (YSI) Technicon Alliance TOC analyser (Dissolved Organic Carbon)

Which data? Results data Database Structure Synthetic data intented to biologists, like groups abundance or concentrations Some raw data require some analysis processing (FCM, Spectral Fluorescence) Multiple importations of sets of results data for a given water sample This constraint comes from the diversity of previous analysis processing Results data can be compared, but methods to analyse those measures too

Structured by Campaign names Database Structure Importation sets (methods used) Types of devices Time High data heterogeneity Some measures are continuous, with distinct sampling rates Synchronisation of all devices is therefore required Locality Either places names or GPS-coordinates Depth Some measures are repeated at various depths (sondes)

Database Structure Meta-data

Database Structure Results-data

DB system Database Implementation Postgresql 9.1, on a Linux server in ULCO university Interface: data import & export tool Through DYMAPHY's website At http://www.dymaphy.eu PHP implementation Data import/export format.csv files (text files, structured by columns) A strict format is required Data description Units, precision indication, description In reference documents, on the website (soon) Database access Public, but registration required (soon) At present time, DB is being fulfilled After DYMAPHY's end will be maintained

Database Web Interface Importation Insert results data into the database From a.csv device table file (Excel, OpenOffice)

Database Web Interface Exportation Extract results from database into.csv file Synchronized data in time/locality/depth Device-Merged data

Database Web Interface Exportation Extraction from database into.csv file

DYMAPHY's Results Database Collected data during DYMAPHY's campaigns Exportation of a single device-merged & synchronised.csv file Public Web access DYMAPHY's Results DataBase Results data: biological, physical, chemical Scientists interested in Phytoplankton Importation of multiple by-device.csv files

Conclusion Conclusion & Perspectives All results data obtained in DYMAPHY's campaign are gathered in this database Public database intented to biologists or data analysis scientists Perspectives Add some more device types Complete data insertion Add more criteria in the export process Finally, open public internet access to DB

end

Data Overview Culture Samples FCM culture samples Species on CAL-1 Species on CAL-2 Species on CAL-3 Mixture on CAL-2 Mixture on CAL-3 From different CytoSense devices Cefas RWS LOG-IFREMER