Thermo Scientific Nunc Products for InVitro Fertilization. security, safety and reproducibility. for your valuable. samples



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Thermo Scientific Nunc Products for InVitro Fertilization security, safety and reproducibility for your valuable samples

The criteria for an IVF product is that it is reliable and does not offer any threat to the viability of your embryos. Nunc IVF products are produced under ISO 13485 to ensure our customers receive a consistent, high quality product with no lot to lot variability. We do the testing so you don t have to

Certifi cations & Testing Mouse Embryo Assay (MEA) Using the 1-cell stage mouse embryo toxicity test, our criteria is that more than 80% of the fertilized eggs should develop to a fully expanded blastocyst after 96 hours. The MEA test is a release test and the product will only be sold if it meets this criteria. Human Sperm Survival Assay (HSSA) To pass the HSSA test, the motility of human sperm should be 70 % after 24 hours of sample preparation. The control sample motility must be at least 50% of the initial swim-up motility for the test to be acceptable. The HSSA test is a release test, and the product will only be sold if it meets this criteria. Certificates of Conformance Testing results are available online for every lot at www.thermoscientifi c.com/nunccertifi cates For user name and password email: certifi cate.nunc@thermofi sher.com Biocompatibility All materials have successfully passed the USP biological reactivity Class VI-500 test (7 days implant) and ISO 10993 standards for cytotoxicity. Sterility Sterility is obtained through irradiation according to ISO 11137 (sterilization of health care products requirements for validation and routine control radiation sterilization). Non-Pyrogenic Batches are tested using a Limulus Amebocyte Lysate (LAL) test. The test is performed on representative samples using the Associates of Cape Cod LAL Detection Kit to quantify gramnegative endotoxins according to our approved test procedure Use of Limulus Amebocyte Lysate for the Detection and Quantifi cation of Bacterial Endotoxins, based on USP <85>. Products are certifi ed non-pyrogenic at less than 20 endotoxin units/device (0.5 endotoxin units/ml).

Collection & Preparation The sensitive nature of IVF calls for extreme care during collection and preparation. Whether working with sperm samples or oocytes, viability is paramount and sample handling is essential. By emphasizing quality, we provide convenient and reliable products for collection and preparation of male and female gametes. Nunc Petri Dishes for IVF Facilitates gas exchange with vented lid Choose the correct size for your application; available in 35mm, 60mm and 90mm sizes Certificates of conformity Release Test (MEA) Nunc 11mL Centrifuge Tube for IVF Minimize product waste with package size of five tubes per pack Quickly identify sample volume with clear graduations Compatible with common centrifuge rotors Certificates of conformity Release Test (MEA and HSSA) Nunc 14mL Round Bottom Tubes Off-gas volatile organic compounds easily with breathable packaging Optimized gas exchange with dual position vent/close cap Certificates of conformity For Research Use Only

Embryo Fertilization, Maturation & Transfer Nunc ICSI and Center Well dishes are CE-marked Class IIa as Medical Devices for IVF and were designed specifi cally for In Vitro Fertilization applications. The fl at bottom design optimizes heat transfer so you can maintain correct temperatures during protocols. Improved handling performance makes the dishes safer and easier to grip, move and manipulate your precious eggs and embryos. Nunc ICSI Dish for IVF Maintain optimal temperature with true fl at bottom Low profi le provides optimal angles during ICSI process Clearly see sample with super polished optical center Increased safety with easy grip design Certifi cates of conformity Release Test (MEA) Nunc Center Well Dish for IVF Perfect angle of reservoir well walls for locating and easy washing of samples Maintain optimal temperature with true fl at bottom Increased safety with easy grip design Certifi cates of conformity Release Test (MEA) Nunc 4 Well Multidish for IVF Ensure proper orientation and prevent cross contamination with notched lid and dish Prevent scratches on well bottoms and maintain optimal temperature with 0.8mm raised edge Certifi cates of conformity Release Test (MEA)

Gamete & Embryo Storage Optimize your frozen embryo transfer success rate with safe and convenient storage solutions for your precious samples. Cryopreservation offers multiple chances to achieve pregnancy from a single oocyte retrieval cycle. Nunc 1.8mL CryoTube for IVF Provides extra safety for valuable samples with internally threaded caps and silicone gasket Easily mark your samples using large writing area Single hand removal of caps when using starfoot rack Certificates of conformity Release Test (MEA and HSSA)

Product specifi cations & ordering information CE marked, FDA cleared and MEA tested Nunc IVF Dishes Sterile Description ICSI Dish Center Well Dish Cat. No. 150265 150260 Surface Non-treated Non-treated Airvent Yes Yes Lid Yes Yes External dimensions, mm 9 x 51 16 x 55 Units per pack/case 1/120 1/120 Nunc IVF 4 Well Dishes Sterile Description 4 Well Dish 4 Well Dish Cat. No. 144444 179830 Surface Nunclon Delta Non-treated Culture area, cm 2 /well 1.9 1.9 External dimensions, mm 66 x 66 66 x 66 Units per pack/case 4/120 4/120 Nunc IVF Petri Dishes Sterile Description Dish 35 x 10 Dish 60 x 15 Dish 90 x 17 Cat. No. 150255 150270 150360 Surface Non-treated Non-treated Non-treated Airvent Yes Yes Yes Culture area, cm 2 /well 8.8 21.5 56.7 External dimensions, mm 35 x 11 54 x 14 88 x 15 Units per pack/case 10/500 10/400 10/150 CE marked and MEA tested Nunc IVF Tubes* Sterile MEA tested - For research use only Nunc 14mL Round Bottom Tubes Description 11mL centrifuge tube 1.8mL Starfoot CryoTube Description 14mL round bottom tube Cat. No. 137860 177280 Cap type Plug Internal thread Material tube/cap Polystyrene/ Polypropylene Polypropylene/ Polypropylene Max. RCF tested 3000 - Bottom shape Conical Round External dimensions, mm 16 x 110 12.5 x 49 Units per pack/case 5/300 - Units per pack/carton/case - 50/450/1800 Cat. No. 150268 Material tube/cap Polystyrene / Polypropylene Cap type Dual function closure Airvent Vent/close Suggested working vol., ml 14 Units per pack/case 10/500 * Not available for sale in US

Other products to consider Biological Safety Cabinets Incubators Ultra Low Temperature Freezers WellWash Versa Centrifuges and Rotors 2011 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. www.thermoscientific.com/ivf Asia: China Toll-free: 800-810-5118 or 400-650-5118; India: +91 22 6716 2200, India Toll-free: 1 800 22 8374; Japan: +81 3 5826 1616; Other Asian countries: 65 68729717 Europe: Austria: +43 1 801 40 0; Belgium: +32 53 73 42 41; Denmark: +45 4631 2000; France: +33 2 2803 2180; Germany: +49 6184 90 6940, Germany Toll-free: 08001-536 376; Italy: +39 02 02 95059 or 434-254-375; Netherlands: +31 76 571 4440; Nordic/Baltic countries: +358 9 329 100; Russia/CIS: +7 (812) 703 42 15; Spain/Portugal: +34 93 223 09 18; Switzerland: +41 44 454 12 12; UK/Ireland: +44 870 609 9203 North America: USA/Canada +1 585 586 8800; USA Toll-free: 800 625 4327 South America: USA sales support: +1 585 899 7198 Countries not listed: +49 6184 90 6940 or +33 2 2803 2180 BRLSPNUNCIVF 0911