International Symposium DFG-Transregio Research Group (FOR 535) together with the Robert Koch Institute Berlin 9th Minisymposium of the German Working Group (DAX) 08. - 09. June 2006, Robert Koch Institute, Berlin Contact: Dr. Joachim Denner Robert Koch Institute Nordufer 20 D-13353 Berlin Germany Tel. +49 30 4547 2800 Fax +49 30 4547 2801 Email: DennerJ@rki.de Secretary +49 30 4547 2802 +49 30 4547 2686 SchroederJ@rki.de MayR@rki.de DAX Deutsche Arbeitsgemeinschaft für
International Symposium DFG-Transregio Research Group (FOR 535) together with the 9th Minisymposium of the German Working Group (DAX) 08. 09. June 2006, Robert Koch Institute, Berlin 2
Program Wednesday, June 07, 2006 19.30 Speaker s dinner Thursday, June 08, 2006 9.00 Opening Prof. Dr. Reinhard Kurth, President, Robert Koch-Institute, Berlin 9.15 9.30 Aims and Status of the German DFG-Transregio Research Group Prof. Dr. Bruno Reichart, Dept. of Cardiac Surgery, Klinikum Grosshadern, Ludwig-Maximilians-University, Munich, Germany Generation and characterisation of multi-transgenic pigs 9.30 10.00 Cardiac : Current Status and Road to the Clinic Prof. Christopher G. A. McGregor, M.B., F.R.C.S., M.D., Mayo Clinic, Saint Marys Hospital, Rochester, USA 10.00 10.20 Transgenic Animals in Experimental Models: Orthotopic Heart Transplantations in the Pig-to-Baboon Model PD Dr. Michael Schmoeckel, Dept. of Cardiac Surgery, Klinikum Großhadern, Ludwig-Maximilians-University, Munich, Germany 10.20 10.50 Coffee break 10.50 11.20 Production of Multi-gene Trangenic Pigs for by Sperm Mediated Gene Transfer (SMGT) Prof. Maria Luisa Lavitrano, Ph.D, Dipartimento Medicina Sperimentale Ambientale Biotecnologie Mediche, Universita di Milano-Bicocca, Monza, Italy 11.20-11.40 Advances in Somatic Cloning of Multi-transgenic Pigs for Research Prof. Dr. Heiner Niemann, Dept. Biotechnology, Institute of Animal Science, Mariensee, Neustadt, Germany 3
11.40-12.00 Transgenic Strategies to Overcome Cellular Rejection of Pig-to-Primate Xenografts Prof. Dr. Eckhard Wolf, Institute of Molecular Animal Breeding and Biotechnology, Gene Centre, Ludwig-Maximilians-University, Munich, Germany 12.00 13.30 Lunch / Press conference 13.30-13.50 Reactivation of Silenced Tetracycline-controlled hrca Constructs in Transgenic Pigs Dr. Wilfried Kues, Dept. Biotechnology, Institute of Animal Science, Mariensee, Neustadt, Germany 13.50-14.10 Targeting of Alpha-Galactosyltransferase with Lentiviral shrna Vectors Prof. Dr. Alexander Pfeifer, Molecular Pharmacology, Dept. of Pharmacy, Centre for Drug Research, Ludwig-Maximilians- University, Munich, Germany 14.10-14.40 Strategies for Viral Gene Delivery into the Cardiovascular System Prof. Dr. Andrew H. Baker, BHF Glasgow, Cardiovascular Research Centre, University of Glasgow, Glasgow, UK 14.40-15.00 AAV a Promising Vector with Secrets Dr. Hildegard Büning, Clinic I for Internal Medicine, University Cologne, Germany 15.00-15.20 AAV-Mediated Gene Transfer into Cardiac Endothelial Cells in Heterotopic Heart Transplantation Models Lars Burdorf, Dept. of Cardiac Surgery, Klinikum Grosshadern, Ludwig-Maximilians-University, Munich, Germany 15.20 16.00 Coffee break Immunological aspects 16.00-16.30 HLA Expression on Porcine Endothelial Cells to Prevent Human Anti- Porcine NK Cell Reactions Prof. Dr. Jörg Seebach, Laboratory for Transplantation Immunology, Dept. of Internal Medicine, University Hospital Zurich, Switzerland 16.30-16.50 Modulation of Human Anti-pig T-cell Responses by Species-Specific Antibodies Prof. Dr. Reinhard Schwinzer, Transplantationslabor, Klinik für Viszeral- und Transplantationschirurgie, Medizinische Hochschule Hannover, Germany 4
16.50-17.10 Analysis of Disorders of Coagulation Following Discordant Xeno-Kidney Perfusion Prof. Dr. Michael Winkler, Klinik für Viszeral- und Transplantationschirurgie, Medizinische Hochschule Hannover, Germany 17.10-18.00 General Discussion 19.00 Buffet/Get-Together Friday, June 09, 2006 Virological aspects 9.00-9.30 PERV Evolution: Receptor and Recombination Dr. Yasuhiro Takeuchi, Wohl Viron Centre, London, UK 9.30-9.50 Detection of Polytropic Replication-Competent PERV-A/-B and of Ecotropic PERV-C in Multitransgenic Pigs PD Dr. Ralf Tönjes, Paul Ehrlich Institute, Langen, Germany 9.50-10.10 Expression of PERV in Multitransgenic Pigs and Inhibition of Virus Transmission by RNA Interference and Neutralising Antibodies Dr. Joachim Denner, Robert Koch Institute, Berlin, Germany 10.10-10.30 General Discussion 10.20 11.00 Coffee break Islet cell and hepatocyte xenotransplantation 11.00-11.30 Recent progress in hepatocyte and islet xenotransplantation PD Dr. Leo H. Bühler, Surgical Research Unit, Department of Surgery, University Hospital, Geneva, Switzerland 11.30-12.00 Pig-to-Human Islet : Why, Where, Who, and When? Prof. Bernhard J. Hering, M.D., Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota, USA 12.00 13.00 Lunch 5
13.00-13.20 Xenogenic Islet Transplantation: A Rocky Road to a Happy End? Prof. Dr. Karin Ulrichs, Chirurgische Klinik und Poliklinik Würzburg, Germany Immunsuppressiva 13.20-13.50 New Immunosuppressiva for Allo- and Prof. Dr. Dietmar Abendroth, Dept. of Surgery, University of Ulm, Ulm, Germany Stem Cell Research 13.50-14.20 Status and Development of Stem Cell Research in the Cardiovascular System Prof. Dr. Bernd Fleischmann, Institute of Physiology I, University Bonn, Bonn, Germany 14.20-14.50 Strategies to Derive Patient-Specific Pluripotential Stem Cells Without Embryos Prof. Dr. Hans Schöler, Max Planck Institute of Molecular Biomedicine, Münster, Germany 14.50-15.30 General Discussion Members and Projects of the German DFG-Transregio Research Group (FOR 535) Project I: Speaker: Public relations and Secretary Prof. Dr. med. Bruno Reichart Dept. of Cardiac Surgery Klinikum Grosshadern, Ludwig-Maximilians-University Marchioninistr. 15, D-81377 München, Germany e-mail: bruno.reichart@med.uni-muenchen.de Project II: Improved transgenic pig lines for xenotransplantation Prof. Dr. med. vet. Heiner Niemann, Dr. Wilfried Kues, Joseph Carnwath, PhD Dept. Biotechnology Institute for Animal Science (FAL), Mariensee D-31535 Neustadt, Germany e-mail: niemann@tzv.fal.de Project III: Transgenic strategies to prevent complement activation and immune cell attack of pig organs in preclinical xenotransplantation models Prof. Dr. med. vet. Eckhard Wolf 1, Dr. rer. nat. Regina Klose 1, Prof. Dr. med. Alexander Pfeifer 2 1 Dept. of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-University, Feodor-Lynen-Str. 25, D-81377 München, Germany 2 Dept. of Pharmacy, Butenandtstr. 5C, D-81377 München e-mail: ewolf@lmb.uni-muenchen.de 6
Project IV: Analysis of xenogeneic activation of the human coagulation system in xenoperfused porcine kidneys and lungs Prof. Dr. med. Michael Winkler 1, Dr. med. Andreas Tiede 2 Dr. med. Lars Friedrich 3 Depts. of 1 Visceral- and Transplantation-Surgery, 2 Haematology and Oncology, 3 Anaesthesiology, Hannover Medical School, Carl-Neuberg- Str. 1, D-30625 Hannover e-mail: winkler.michael@mh-hannover.de Project V: Multitransgenic animals and in vivo gene transfer in experimental xenotransplantation models Priv.-Doz. Dr. med. Michael Schmoeckel 1, Prof. Dr. med. Dr. med. vet. Claus Hammer 2, Priv.-Doz. Dr. med. Frank Christ 3 Depts. of Cardiac Surgery 1, Surgical Research 2 and Anesthesiology 3 Klinikum Grosshadern, Ludwig-Maximilians-University Marchioninistr. 15, D-81377 München, Germany e-mail: michael.schmoeckel@med.uni-muenchen.de Project VI: Monoclonal antibodies to porcine cell surface molecules for the control of human anti-pig cellular immune responses Prof. Dr. rer. nat. Reinhard Schwinzer Transplantationslabor, Dept. of Visceral- and Transplantation-Surgery, Medizinische Hochschule Hannover, Carl-Neuberg- Str. 1, D-30625 Hannover e-mail: schwinzer.reinhard@mh-hannover.de Project VII: Recombinant adeno-associated virus (raav)-mediated transgene delivery into porcine organs to allow immunological modification of xenografts Prof. Dr. med. Michael Hallek, Dr. Hildegard Büning, Dr. med. Ulrich Hacker, Dr. Susan King Internal Medicine I, University Cologne, Joseph-Stelzmann-Str. 9, D-50924 Koeln e-mail: Hildegard.Buening@uk-koeln.de Project VIII: Evaluation of the risk posed by porcine endogenous retroviruses (PERVs) from transgenic pigs Priv.-Doz. Dr. rer. nat. Ralf R. Tönjes 1 Dr. rer. nat. Joachim Denner 2 1 Paul Ehrlich Institut Langen, Paul-Ehrlich-Str. 51-59, D-62355 Langen e-mail: toera@pei.de 2 Robert Koch Institute, Nordufer 20, D-13353 Berlin e-mail: DennerJ@rki.de 7