Laboratory Acquired Infections (LAIs)

Transcription

1 LimeSurvey - Laboratory Acquired Infections (s) Page 5/06/0 Laboratory Acquired Infections (s) Online survey about 'Laboratory Acquired Infections' (s) for the attention the personnel Dear Madam, Sir On request the Flemish authorities, the SBB (the Biosafety and Biotechnology Unit the Scientific Institute Public Health) has developed a survey in the interest mapping and evaluating the risk for laboratory-acquired infections (s) related to bio-incidents with pathogenic organisms. Bio-incidents are defined as all irregularities that occur while handling these organisms, caused by technical failure or human errors or simply by accident. The term laboratory-acquired infections (s) refers to all direct or indirect human infections with or without the onset symptoms following exposure to pathogenic organisms (genetically modified or not) in the laboratory. There are many scientific publications on bio-incidents and s. However, very few data are available regarding the incidence in Belgium & Flanders. Our aim is to obtain more information about this issue and the possible underlying causes. Completing this survey happens anonymously and will only take about 5 minutes. Thank you very much for your participation A report this survey will be sent to you More information on biosafety and s can be found on our website ( Dienst Bioveiligheid en Biotechnologie Service Biosécurité et Biotechnologie Operationele Directie Expertise, Dienstverlening en Klantenrelaties Direction Opérationnelle Expertise, Prestations de service et relations clients Rue Juliette Wytsmanstraat Brussels T F [email protected] Wetenschappelijk Instituut Volksgezondheid - Institut Scientifique de Santé Publique There are 4 questions in this survey Laboratory Acquired Infections (s)

2 LimeSurvey - Laboratory Acquired Infections (s) Page 5/06/0 []. In which type organization are you employed? * Please choose only one the following: Public institution or university Private company

3 LimeSurvey - Laboratory Acquired Infections (s) Page 5/06/0 []. How would you classify the sector/branch industry that you work in? (Multiple answers possible) * Please choose all that apply: R&D Diagnostics (medical veterinary) Quality Control ( environmental samples, food samples, pharmaceuticals) Production Education []. The focus your work is mainly on: * Please choose only one the following: (Bio)medical (Human) Veterinary (Animals) Agricultural, ornamental plant cultivation, agrobiotechnology (Plants) 4 [4]4. You are employed as: (Multiple answers possible) * Please choose all that apply: Lab technician Researcher Short-term stagiair or student (< month ) Long-term stagiair or student (> month) Animal caretaker Andere: 5 [5new]5. In which type laboratory do you work? (Multiple answers possible) * Please choose all that apply: L, lab containment level L, lab containment level L, lab containment level A, animal facility containment level A, animal facility containment level A, animal facility containment level G, greenhouse containment level G, greenhouse containment level G, greenhouse containment level I don't know Other: 6 [6]6. Which type activities are you mainly doing? (Multiple answers possible) * Please choose all that apply: Cell culture Animal care In vivo research(small and large animals) Microbiology Microscopy Education (practical lessons) Serology or hematology (diagnostics) : Transmissible spongiform encephalopathies

4 LimeSurvey - Laboratory Acquired Infections (s) Page 4 5/06/0 (research) Maintenance, management collection Cloning Other: 7 [7 new]which manipulation/activity that you perform yourself do you consider the most risky for your health? Please also indicate which (micro-)organism is involved in this manipulation/activity. ( eg injection mice with lentiviral vectors, or culturing Mycobacterium tuberculosis, etc...) * 8 [8 new]9. Are you aware any 's (laboratory acquired infections, resulting from a contamination or more employees (including yourself) in your organization over the last 5 years? * Please choose only one the following: Yes No 9 [8.]How many 's are you aware? * ((8 new.naok == "Y")) 0 [8. NEW]Which organisms or biologic agents were involved in the (s)?(bv. Trypanosoma brucei gambiense) * ((8..NAOK == "")) Please write your answer(s) here: [8. NEW]Which organisms were involved in the (s)? (eg. Trypanosoma brucei gambiense, eg. influenza virus,...) * ((8..NAOK == "")) Please write your answer(s) here: [8. NEW]Which organisms were involved in the (s)? (eg. Trypanosoma brucei gambiense, eg. influenza virus,...) * ((8..NAOK == ""))

5 LimeSurvey - Laboratory Acquired Infections (s) Page 5 5/06/0 Please write your answer(s) here: [8. 4 NEW]Which organisms were involved in the (s)? (eg. Trypanosoma brucei gambiense, eg. influenza virus,...) * ((8..NAOK == "4")) Please write your answer(s) here: 4 4 [8. 5 NEW]Which organisms were involved in the (s)? (eg. Trypanosoma brucei gambiense, eg. influenza virus,...) * ((8..NAOK >= "5")) Please write your answer(s) here: 4 5 If you have knowledge more than 5 (s), please indicate the 5 most recent ones (over the last 5 years). 5 [8. new]which types biologic agents were involved? * ((8..NAOK == "")) Laboratory technician Wie werd er besmet? // Who was contaminated? Waar gebeurde de besmetting? // Where did the contamina Stagiair / Stagiair / student student for L, L, L, for short longer laboratory laboratory laboratory A, animal A, animal period period Animal I facility facility (< (> care don't containment containment containment containment containment Researcher month) month) taker know Other level level level level level 6 [8. new] * ((8..NAOK == "")) Wie werd er besmet? // Who was contaminated? Waar gebeurde de besmetting? // Where did the contamina Stagiair / Stagiair / student

6 LimeSurvey - Laboratory Acquired Infections (s) Page 6 5/06/0 Laboratory technician Researcher student for short period (< month) for longer period (> month) Animal care taker I don't know Other L, laboratory containment level L, laboratory containment level L, laboratory containment level A, animal facility containment level A, animal facility containment level 7 [8. new] * ((8..NAOK == "")) Laboratory technician Wie werd er besmet? // Who was contaminated? Waar gebeurde de besmetting? // Where did the contamina Stagiair / Stagiair / student student for L, L, L, for short longer laboratory laboratory laboratory A, animal A, animal period period Animal I facility facility (< (> care don't containment containment containment containment containment Researcher month) month) taker know Other level level level level level 8 [8. 4 new] * ((8..NAOK == "4")) 4 Laboratory technician Wie werd er besmet? // Who was contaminated? Waar gebeurde de besmetting? // Where did the contamina Stagiair / Stagiair / student student for L, L, L, for short longer laboratory laboratory laboratory A, animal A, animal period period Animal I facility facility (< (> care don't containment containment containment containment containment Researcher month) month) taker know Other level level level level level 9 [8. 5 new] * ((8..NAOK >= "5")) 4 5 Laboratory technician Wie werd er besmet? // Who was contaminated? Waar gebeurde de besmetting? // Where did the contamina Stagiair / Stagiair / student student for L, L, L, for short longer laboratory laboratory laboratory A, animal A, animal period period Animal I facility facility (< (> care don't containment containment containment containment containment Researcher month) month) taker know Other level level level level level 0 [8.. "Anders" pers]of which other persons, also involved in the, are you aware? * ((8. new_sq00_0.naok == "a")) or ((8. new_sq00_0.naok == "a")) or ((8. new_sq00_0.naok == "a")) or ((8. new_sq00_0.naok == "a")) or ((8. new_sq00_0.naok == "a")) or ((8. new_sq00_0.naok == "a")) or ((8. 4 new_sq00_0.naok == "a")) or ((8. 4 new_sq00_0.naok == "a")) or ((8. 5 new_sq004_0.naok == "a")) or ((8. 5 new_sq005_0.naok == "a")) or ((8. 5 new_sq00_0.naok == "a")) or ((8. 5 new_sq00_0.naok == "a")) or ((8. 5 new_sq00_0.naok == "a")) or ((8. 4 new_sq00_0.naok == "a")) or ((8. 4 new_sq004_0.naok == "a"))

7 LimeSurvey - Laboratory Acquired Infections (s) Page 7 5/06/0 [8.. 'andere plaats]of which other location, also involved in the, are you aware? * ((8. new_sq00_.naok == "a")) or ((8. new_sq00_.naok == "a")) or ((8. new_sq00_.naok == "a")) or ((8. new_sq00_.naok == "a")) or ((8. new_sq00_.naok == "a")) or ((8. new_sq00_.naok == "a")) or ((8. 4 new_sq00_.naok == "a")) or ((8. 4 new_sq00_.naok == "a")) or ((8. 4 new_sq00_.naok == "a")) or ((8. 4 new_sq004_.naok == "a")) or ((8. 5 new_sq00_.naok == "a")) or ((8. 5 new_sq00_.naok == "a")) or ((8. 5 new_sq00_.naok == "a")) or ((8. 5 new_sq004_.naok == "a")) or ((8. 5 new_sq005_.naok == "a")) [8.4 ]Which types biologic agents were involved? * ((8..NAOK == "")) In welke context gebeurde de besmetting? // In which context did the contamination happen? Cell culture Animal care In vivo research (small and large test animals) Microbiology Microscopy Education (practicum) Serology or hematology (diagnostics) (research) Maintenance collection Cloning Transpor [8.4 new] * ((8..NAOK == "")) In welke context gebeurde de besmetting? // In which context did the contamination happen? Cell culture Animal care In vivo research (small and large test animals) Microbiology Microscopy Education (practicum) Serology or hematology (diagnostics) (research) Maintenance collection Cloning Transpor 4 [8.4 new]which types biologic agents were involved? * ((8..NAOK == "")) In welke context gebeurde de besmetting? // In which context did the contamination happen? Cell culture Animal care In vivo research (small and large test animals) Microbiology Microscopy Education (practicum) Serology or hematology (diagnostics) (research) Maintenance collection Cloning Transpor

8 LimeSurvey - Laboratory Acquired Infections (s) Page 8 5/06/0 5 [8.4 4 new] * ((8..NAOK == "4")) In welke context gebeurde de besmetting? // In which context did the contamination happen? 4 Cell culture Animal care In vivo research (small and large test animals) Microbiology Microscopy Education (practicum) Serology or hematology (diagnostics) (research) Maintenance collection Cloning Transpor 6 [8.4 5 new] * ((8..NAOK >= "5")) In welke context gebeurde de besmetting? // In which context did the contamination happen? 4 5 Cell culture Animal care In vivo research (small and large test animals) Microbiology Microscopy Education (practicum) Serology or hematology (diagnostics) (research) Maintenance collection Cloning Transpor 7 [8.4. new]which other context are you aware? * ((8.4 _SQ00_0.NAOK == "a")) or ((8.4 new_sq00_0.naok == "a")) or ((8.4 new_sq00_0.naok == "a")) or ((8.4 new_sq00_0.naok == "a")) or ((8.4 new_sq00_0.naok == "a")) or ((8.4 4 new_sq00_0.naok == "a")) or ((8.4 4 new_sq00_0.naok == "a")) or ((8.4 4 new_sq00_0.naok == "a")) or ((8.4 4 new_sq004_0.naok == "a")) or ((8.4 5 new_sq00_0.naok == "a")) or ((8.4 5 new_sq005_0.naok == "a")) or ((8.4 5 new_sq004_0.naok == "a")) or ((8.4 5 new_sq00_0.naok == "a")) or ((8.4 5 new_sq00_0.naok == "a")) or ((8.4 new_sq00_0.naok == "a")) 8 [8.4. new]which other incident do you know that has lead to an infection? * ((8.4 _SQ00_.NAOK == "a")) or ((8.4 new_sq00_.naok == "a")) or ((8.4 new_sq00_.naok == "a")) or ((8.4 new_sq00_.naok == "a")) or ((8.4 new_sq00_.naok == "a")) or ((8.4 4 new_sq00_.naok == "a")) or ((8.4 4 new_sq00_.naok == "a")) or ((8.4 4 new_sq00_.naok == "a")) or ((8.4 4 new_sq004_.naok == "a")) or ((8.4 5 new_sq00_.naok == "a")) or ((8.4 5 new_sq00_.naok == "a")) or ((8.4 5 new_sq00_.naok == "a")) or ((8.4 5 new_sq004_.naok == "a")) or ((8.4 5 new_sq005_.naok == "a")) or ((8.4 new_sq00_.naok == "a"))

9 LimeSurvey - Laboratory Acquired Infections (s) Page 9 9 [8.5 new]which types biologic agents were involved? * ((8..NAOK == "")) Was er sprake van transmissie naar andere personen (vb familie, collega s ) //Do you know if it was transmitted to another person (eg family member, colleague...)? Is effectief aangetoond dat de infectie het gevolg was van een besmetting op de werklvoer? //Has it been proven that the resulted from a contamination at work? Yes No I don t know Yes No I don t know 0 [8.5 new] * ((8..NAOK == "")) Was er sprake van transmissie naar andere personen (vb familie, collega s ) //Do you know if it was transmitted to another person (eg family member, colleague...)? Is effectief aangetoond dat de infectie het gevolg was van een besmetting op de werklvoer? //Has it been proven that the resulted from a contamination at work? Yes No I don t know Yes No I don t know [8.5 new] * ((8..NAOK == "")) Was er sprake van transmissie naar andere personen (vb familie, collega s ) //Do you know if it was transmitted to another person (eg family member, colleague...)? Is effectief aangetoond dat de infectie het gevolg was van een besmetting op de werklvoer? //Has it been proven that the resulted from a contamination at work? Yes No I don t know Yes No I don t know [8.5 4 new] * ((8..NAOK == "4")) 4 Was er sprake van transmissie naar andere personen (vb familie, collega s ) //Do you know if it was transmitted to another person (eg family member, colleague...)? Is effectief aangetoond dat de infectie het gevolg was van een besmetting op de werklvoer? //Has it been proven that the resulted from a contamination at work? Yes No I don t know Yes No I don t know [8.5 5 new] * ((8..NAOK >= "5")) 4 5 Was er sprake van transmissie naar andere personen (vb familie, collega s ) //Do you know if it was transmitted to another person (eg family member, colleague...)? Is effectief aangetoond dat de infectie het gevolg was van een besmetting op de werklvoer? //Has it been proven that the resulted from a contamination at work? Yes No I don t know Yes No I don t know 5/06/0

10 LimeSurvey - Laboratory Acquired Infections (s) Page 0 5/06/0 4 [9 new] 9. Bio-incidents* Not every bio-incident* leads to an actual infection, but it is interesting to know what type bioincidents occur frequently in Belgium. Are you aware any bio-incidents that have ocurred in your organization within the last 5 years? * Please choose only one the following: Yes No (* A bio-incident is a term to indicate all kinds irregularities that can happen while working with biologic agents: eg cut accidents and accidental injection, falling and breaking containers, flasks,etc...) 5 [9. new]do you know if one the possible bio-incidents occurred in your organization (over the last 5 years) _ Select the applicable option and on the right side you can give the number or amount these incidents that have occurred. ((9 new.naok == "Y")) Please choose all that apply and provide a comment: Spills Cutting / needle stick accident Bites and scratches (animals) Splashes Falling recipient Breaking recipient Careless handling Technical failure equipment ( eg. badly functioning safety cabinet, centrifuge,...) Not wearing personal protection equipment ( gloves, goggles...) Technical failure infrastructure (eg. HVAC, air filter,...) Not known Other (on the right side you can give the number or amount these incidents that have occurred) 6 [9. new]wgich other type op bio-incidents can you remember? * ((9. new_0.naok == "Y"))

11 LimeSurvey - Laboratory Acquired Infections (s) Page 5/06/0 7 [0.]Are you aware the existence an internal procedure for dealing with a bio-incident? * Please choose only one the following: Yes No 8 [0 new]0. To your opinion, is a bio-incident spontaneously notified by the personnel? * Please choose only one the following: Yes No 9 [0.N]To your opinion, what are the reasons for not reporting such an incident? (Multiple answers possible) * ((0 new.naok == "N")) Please choose all that apply: The bio-incident is not serious enough to report it Fear for reactions colleagues Fear for reactions superior Other: 40 [0.Y new] To whom is the incident reported? (Multiple answers possible) * Please choose all that apply: Biosafety ficer/coordinator Prevention advisor Occupational health ficer Lab responsible Colleague(s) Nobody Other: 4 [ new]questions on spill procedures * Does your lab have a procedure for the cleaning spills? Are there any spill kits available? Are there specific spill trainings organized? Yes No I don't know 4 [ new]as mentioned earlier, a bio-incident is ten the result several different factors. How high would you rate the next conditions as underlying causes bio-incidents? ( 0=low, 0= high) * Not enough experience Not enough training received Not enough follow-up Too much work Lack space Lack adapted equipment, materials Lack knowledge about the possible risks that come with a certain activity Lack attention /absence mind, being distracted Not respecting certain biosafety practices

12 LimeSurvey - Laboratory Acquired Infections (s) Page 5/06/0 (0= low, 0= high) 4 []Thank you! This is the end this survey. If you have remarks or suggestions on this survey, you are invited to write them here.

13 LimeSurvey - Laboratory Acquired Infections (s) Page 5/06/0 Thank you for participating in this survey Do not hesitate to contact us if you have any furher questions, [email protected] :00 Submit your survey. Thank you for completing this survey.