BS. Agricultural and Biological Sciences, Escuela Superior Politecnica del Litoral (ESPOL), Guayaquil-Ecuador
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1 1 EDUCATION Robert A. Alvarez Quinto Plant Virology Program. Centro de Investigaciones Biotecnológicas del Ecuador (CIBE-ESPOL) Km 30.5 Vía Perimetral, Campus Gustavo Galindo - ESPOL Guayaquil - Ecuador (EC090112) (593) Sept BS. Agricultural and Biological Sciences, Escuela Superior Politecnica del Litoral (ESPOL), Guayaquil-Ecuador JOB EXPERIENCE Aug to Nov Internship. USDA-HCRU (Horticultural Crop Research Unit), Corvallis Oregon. Genomic characterization of RNA viruses infecting tropical crops from Ecuador. Jan to present. Research Assistant, Plant Virology Program at Centro de Investigaciones Biotecnológicas del Ecuador (CIBE-ESPOL), Guayaquil-Ecuador - Mealy bug wilt of pineapple (MWP) virus detection and field assays. - Characterization of a new Umbra-like virus from papaya crop. - Detection and characterization of plant viruses in melon, watermelon and Solanum betaceum. Sept to Dec Visiting Researcher. Plant Virology Unit - International Center for Tropical Agriculture, CIAT CGIAR. Palmira- Colombia Project: Unraveling cassava frog skin disease in cassava Colombia
2 2 - Use of indicator plant species for building a profile of response symptoms for an uncharacterized isolate of Cassava green mottle virus - CaGMV (a Tentative Comoviridae). - Primer walking and re-sequencing of putative viral sequences obtained by sirna deep sequencing in cassavainfected plants. - Development of multiplex PCR for diagnostic of viral absence in cassava in vitro-obtained plants. - Preliminary standardization of extraction protocols for high quality RNA isolation from banana and rice leaves. Oct Sept BS Thesis. CIBE-ESPOL Projects - Virus survey in melon across the main production areas of Ecuador. - Study of the effect of BIOL (manure-derived liquid compound) on melon plants infected by SqMV. Oct Oct Undergraduate internship. CIBE-ESPOL. Projects Evaluation of the incidence of TSVW, WMV, SqMV and PRSV-W in tomato and melon crops in Ecuador by serological and molecular methods. GRANTS AND AWARDS CTBE Embassy of Belgium in Ecuador Scholarship (2012) Visiting Researcher at Plant Virology Unit (CIAT) International Center for Tropical Agriculture. Duration: 3 Months CICYT-ESPOL Grant for undergraduate thesis. (2010) Preliminary effect of BIOL (manure-derived compound) on symptoms caused by Squash Mosaic Virus (SqMV) infecting Cucumis melo. CIBE ESPOL Duration: 9 Months
3 3 PROFESSIONAL MEETINGS AND PRESENTATIONS August 9-13, 2014 American Phytopathological Society (APS) Annual meeting. Minnesota, MN A new Umbravirus possibly associated with papaya ringspot disease in Ecuador June 9-12, 2014 II International Congress of Biotechnology and Biodiversity (CIIB- 2014). Guayaquil - Ecuador Occurrence of Melon yellow spot virus and its association with watermelon foxtail disease in Ecuador August 10-14, 2013 American Phytopathological Society (APS) Annual meeting. Austin, TX. October 1-5, 2013 Poster: A new Raspberry bushy dwarf virus isolate from Ecuador exhibits an aberrant genetic feature XXII PERUVIAN CONGRESS AND XVII LATIN AMERICAN CONGRESS OF PHYTOPATHOLOGY (CPLAF-2013). Lambayeque - Peru Sequencing of a new raspberry bushy dwarf virus isolate infecting blackberry (Rubus glaucus) in Ecuador.
4 4 SKILLS Discover of RNA viruses by double stranded RNA (dsrna) extraction and sequencing. Plant virus diagnosis based on RT-PCR. Nucleic acid extraction RNA, DNA. Bioinformatics tools (GENEIOUS) for virus genomics: primer design, sequences assembly and phylogenetic analysis. Perform sample collection at field. Establish assay at greenhouse and field. Plant infection by mechanical inoculation or grafting at greenhouse. Insights about next generation sequencing approach for virus discovery, including sirna deep sequencing. PUBLICATIONS Quito- Avila D.F, Alvarez R.A., Ibarra,, M.A., Martin, R.R. (2015). Detection and partial genome sequence of a new umbra- like virus of papaya discovered in Ecuador. European Journal of Plant Pathology. Submitted. Alvarez RA, Martin RR, Quito- Avila DF, First report of Pineapple mealybug wilt associated virus- 1 in Ecuador. New Disease Reports 31, 15. [ Quito- Avila, D.F., Ibarra, M.A., Alvarez, R.A., Peralta, E.L., and Martin, R.R A Raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA 2. Arch of Virol. 159: Quito- Avila, D.F., Ibarra, M.A., Alvarez, R.A., Peralta, E.L., Martin, R.R. and Ochoa, J Detection and Occurrence of Melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region. Eur. J. Plant Pathology. 140: Quito- Avila, D.F., Peralta, E.L., Ibarra, M.A., Alvarez, R., Ratti, M.F., Espinoza, L. and Martin, R.R First report of Raspberry bushy dwarf virus in blackberry in Ecuador. Plant Disease. 97:1003. ( PDN) Quito- Avila, D.F., Ibarra, M.A., Alvarez, R., Ratti, M.F., Espinoza, L., Cevallos- Cevallos, J.M. and Peralta, E.L First report of Banana bract mosaic virus in 'Cavendish' banana in Ecuador. Plant Disease. 97:1003. ( PDN)
5 5 Alvarez, R.A, Espinoza, L., Ruiz, O., Peralta, E. Efecto de los Biofertilizantes Líquidos de Producción Local Bioles, sobre el Desarrollo de Síntomas Causados por el Virus del Mosaico de la Calabaza (SqMV) en el Cultivo de Melón (Cucumis melo L.) var. Edisto en Condiciones de Invernadero. Repositorio de la Escuela Superior Politécnica del Litoral. L. Espinoza- Lozano; A.M. Campusano; A. Ibarra; R. A. Alvarez; J. Paredes; E. L. Peralta Diagnóstico e identificación de nuevas especies virales en cultivos de tomate y melón en Ecuador. Fitopatología Colombiana. Vol 35:1 (Suplemento especial) Pg Espinoza, L.; Jama, M.; Álvarez, R.; Paredes, J.; Peralta, E. L Determinación de géneros y especies virales que se encuentran afectando cucurbitáceas en la zona de Pedro Carbo, provincia del Guayas. Revista tecnológica Espol RTE, Vol. 23, N. 1, REFERENCES. Diego Fernando Quito-Avila, Ph.D Head of Virology Program Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Km 30.5 Via Perimetral, Campus Gustavo Galindo Guayaquil-Ecuador diego.quito.avila@gmail.com Telephone: (593) Ing. Esther Peralta, Ph D. Head of Centro de Investigaciones Biotecnologicas del Ecuador (CIBE), Km 30.5 Via Perimetral, Campus Gustavo Galindo Guayaquil-Ecuador estherlilia@gmail.com Telephone: (593) Wilmer Cuellar, Ph.D Head of Virology Unit International Center for Tropical Agriculture (CIAT) Palmira Colombia w.cuellar@cgiar.org Robert A. Alvarez- Quinto
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