Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology

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1 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology FORMATEX Microbiology Series Nº 2 VOL. 2 Edited by A. Méndez-Vilas

2 Vol. 2 ISBN-13: FORMATEX C/ Zurbarán 1, 2º - Oficina Badajoz Spain [email protected] Printed in Spain

3 CONTENTS VOL. 1 Introduction... XVII Environmental Microbiology, Geomicrobiology, Soil Microbiology, Biocontrol Waterborne transmission of Cryptosporidium and Giardia: detection, surveillance and implications for public health D. Carmena Purification of enzymes related to host penetration and pathogenesis from entomopathogenic fungi S. Ali, S.X. Ren, Z. Huang and J.H. Wu Polyamines in cyanobacteria: biosynthesis, transport and abiotic stress response Aran Incharoensakdi, Saowarath Jantaro, Wuttinun Raksajit, and Pirkko Mäenpää Adaptation to oxidative stress by Gram-positive bacteria: the redox sensing system HbpS-SenS-SenR from Streptomyces reticuli Matthew R. Groves, and Darío Ortiz de Orué Lucana Pioneer communities in the forefields of retreating glaciers: how microbes adapt to a challenging environment Lazzaro A, Franchini AG, Brankatschk R, Zeyer J Molecular Microbial Ecology of Marmara Sea Sediments O. Ince, M. Kolukirik and B.K. Ince Sludge oomph: harnessing the power of sediment microbiota Alexandra S. Alves, Bruno M. Fonseca and Ricardo O. Louro Comparative analysis of two phytohormone and siderophores rhizobacteria producers isolated from heavy metal contaminated soil and their effect on Lens esculenta growth and tolerance to heavy metals M.O. Franco-Hernández, S. Montes-Villafàn, M. Ramírez-Melo, A. Rodríguez-Dorantes, A. Rodriguez- Tovar, A.N. Ruíz-Flores, M.S. Vásquez-Murrieta and A. Ponce-Mendoza Capturing the potential of Haloalkaliphilic bacteria from the saline habitats through culture dependent and metagenomic approaches S. P. Singh, M. K. Purohit, V.H. Raval, S. Pandey, V. G. Akbari and C. M. Rawal Characteristics of Extremophylic Fungi from Chernobyl Nuclear Power Plant Т. Belozerskaya, K. Aslanidi, А. Ivanova, N. Gessler, A. Egorova, Yu. Karpenko, and S. Olishevskaya Induction of secondary symbiosis between the ciliate Paramecium and the green alga Chlorella Y. Kodama, and M. Fujishima III

4 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology A. Méndez-Vilas (Ed.) Isolation and characterization of bioluminescent bacteria from marine environments of Puerto Rico J. Malave-Orengo, Eva N. Rubio-Marrero and C. Rios-Velazquez Isolation and characterization of purple non-sulfur anoxyphototropic bacteria from two microecosystems: tropical hypersaline microbial mats and bromeliads phytotelmata K. Soto-Feliciano, M. De Jesús, J. Vega- Sepúlveda and C. Ríos-Velázquez Characterization of blue pigmented bacteria isolated from Puerto Rico V. Cardona-Cardona, David Arroyo, J. Scellekens and C. Rios-Velazquez Bacterial Extracellular Enzymatic Activity in Globally Changing Aquatic Ecosystems A. Cunha, A. Almeida, F.J.R.C. Coelho, N. C. M. Gomes, V. Oliveira, and A. L. Santos Bacteria in molluscs: good and bad guys J.L. Romalde, and J.L. Barja Antioxidative defense systems of anaerobic sulfate-reducing microorganisms A. Brioukhanov, L. Pieulle and A. Dolla Evaluation of sol-gel silica matrices as inoculant carriers for Mesorhizobium spp. cells G. S. Alvarez, F. L. Pieckenstain, M. F. Desimone, M. J. Estrella, O. A. Ruiz and L. E. Díaz Biological fertilization Mohammad Miransari Metabolic strategies deployed by Pseudomonas fluorescens to combat metal pollutants: Biotechnological prospects Joseph Lemire, Christopher Auger, Adam Bignucolo, Varun P. Appanna, and Vasu D. Appanna Adsorptions of DNA molecules by soils and variable-charged soil constituents K. Saeki and T. Kunito Mechanisms of infection used by Xanthomonas axonopodis pv. citri in citrus canker disease N. Gottig, B.S. Garavaglia, C.G. Garofalo, T. Zimaro, G.G. Sgro, F.A. Ficarra, G. Dunger, L.D. Daurelio, L. Thomas, C. Gehring, E.G. Orellano and J. Ottado Proteomics of fungal plant pathogens: the case of Botrytis cinerea R. González Fernández, and J.V. Jorrín Novo The universe of basidiomycetous ground fungi G. Gramss Management of the Mycorrhizal Soil Infectivity to Improve Reforestation Program Achievements in Sahelian Ecosystems Arsene Sanon, Fatou Ndoye, Ezékiel Baudoin, Yves Prin, Antoine Galiana and Robin Duponnois Phytophthora cinnamomi suppressive soils B. Keen and T. Vancov IV FORMATEX 2010

5 Rhizospheric plant-microbe interactions that enhance the remediation of contaminated soils E. Amora-Lazcano, L.A. Guerrero-Zúñiga, A. Rodriguez-Tovar, A. Rodriguez-Dorantes and M.S. Vasquez-Murrieta Investigating the role of microorganisms in soil seed bank management J. Chee-Sanford, and X. Fu On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen Belén Álvarez, Elena G. Biosca, and María M. López Molecular diversity and enzymatic potential of salt-tolerant alkaliphilic actinomycetes S. P. Singh, J.T. Thumar, S.D. Gohel, and M. K. Purohit Fate and environmental impact of the recently marketed herbicide Mesotrione: coupling biological and chemical studies for a global overview Isabelle Batisson, Martine Sancelme, Clarisse Mallet, and Pascale Besse-Hoggan A challenge to mushroom growers: the green mould disease of cultivated champignons L. Kredics, L. García Jimenez, S. Naeimi, D. Czifra, P. Urbán, L. Manczinger, C. Vágvölgyi, and L. Hatvani Nitrogen metabolism in ectomycorrhizal fungi: fhant-ac gene regulation in Laccaria bicolor Minna J. Kemppainen and Alejandro G. Pardo Biological soil quality indicators: a review Martinez-Salgado M,M., Gutiérrez-Romero, V., Jannsens, M., Ortega-Blu, R Fungal weathering and implications in the solubilization of metals from soil and from asbestos fibres S. Daghino, E. Martino and S. Perotto Lecanicillium muscarium as microbial insecticide against whitefly and its interaction with other natural enemies S.X. Ren, S. Ali, Z. Huang and J.H. Wu Novel Fermentation media for the production of mosquito pathogenic bacilli in mosquito control Subbiah POOPATHI Antimicrobials, Susceptibility, Resistance, Biosynthesis of antibiotics Inorganic nano mental oxides used as anti-microorganism agents for pathogen control Lu-E Shi, Liangying Xing, Baochao Hou, Hongjuan Ge, Xiaofeng Guo and Zhenxing Tang Antimicrobial efficacy and systematic use of disinfectants K. Steinhauer Identification and bioactive properties of endophytic fungi isolated from phyllodes of Acacia species H.B.Q. Tran, J.M. McRae, F. Lynch, and E.A. Palombo V

6 Weapons of a pathogen: Proteases and their role in virulence of Pseudomonas aeruginosa R. Hoge, A. Pelzer, F. Rosenau, S. Wilhelm Antimicrobial activity of secondary metabolites and lectins from plants P.M.G. Paiva, F.S. Gomes, T.H. Napoleão, R.A. Sá, M.T.S. Correia and L.C.B.B. Coelho Nanobiotechnology: A new strategy to develop non-toxic antimicrobial textiles Isabel C. Gouveia Pitfalls of antimicrobial susceptibility testing of enterococci isolated from farming broilers by the disk diffusion method Manuela Oliveira, Verónica Santos, Amélia Fernandes, Sandro Filipe Nunes, Fernando Bernardo, and Cristina Lobo Vilela Multi-functions of photodynamic pigments in ciliated protozoans T. Matsuoka, H. Kotsuki and Y. Muto Blueberries: Genotype-dependent variation in antioxidant, free-radical scavenging, and prebiotic activities A.L. Molan, M. A. Lila and G. Ravindran Microbial superoxide dismutase enzyme as therapeutic agent and future gene therapy Hatem M. El Shafey, Saleh A. Bahashwan, Abdulaziz A. Alghaithy and Samah Ghanem Fruit and vegetable peels strong natural source of antimicrobics S. Chanda, Baravalia Y, Kaneria M and Rakholiya K Novel leads from herbal drugs for infectious skin diseases S. Chanda and Y. Baravalia Inhibitory Effect of Green Onion on the Growth of Aspergillus parasiticus Jong-Gyu Kim Peptidoglycan hydrolases as novel tool for anti-enterococcal therapy Lenka Maliničová, Mária Piknová, Peter Pristaš, and Peter Javorský Seaweeds: A novel, untapped source of drugs from sea to combat Infectious diseases S. Chanda, Dave R, Kaneria M and Nagani K How do bees prevent hive infections? The antimicrobial properties of propolis H. Fokt, A. Pereira, A. M. Ferreira, A. Cunha, and C. Aguiar Novel Scientific Research in the College Classroom: Identification of Antibiotic Resistant Pseudomonas Species from a Wastewater Treatment Plant K. F. Picardo and D. W. Giroux Comparative analysis of 16S rrna methyltransferases conferring resistance to aminoglycoside antibiotics in producing strains S. Vojnović, T. Ilić-Tomić, M. Savić, S. Bajkić, I. Morić, and B. Vasiljević VI

7 Antibiotic resistance dissemination and sewage treatment plants K.L. Jury, T. Vancov, R.M. Stuetz, S.J. Khan Analysis of plasmids as genetic support of the class D carbapenemase OXA-40 in multidrug-resistant Acinetobacter baumannii isolates L. Gallego Global resistance trends and the potential impact of Methicillin Resistant Staphylococcus aureus (MRSA) and its solutions S. Chanda, B.R.M. Vyas, Y. Vaghasiya, H. Patel Bacillus spp. thermal resistance and validation in soups A. Rodríguez-Lozano, M. Campagnoli, K. Jewel, F. Monadjemi and J.E. Gaze All Stressed Out: Mycobacterial Responses to Stress Beatrice Saviola Defensin-like antifungal proteins secreted by filamentous fungi L. Galgóczy, L. Kovács and Cs. Vágvölgyi Streptomyces developmental cycle and secondary metabolite production A. Manteca and J. Sanchez Endophytic fungi for producing bioactive compounds originally from their host plants J. Zhao, L. Zhou, J. Wang, T. Shan, L. Zhong, X. Liu, and X. Gao Evolution of fungal β-lactam biosynthesis gene clusters Carlos García-Estrada, Francisco Fierro, and Juan-Francisco Martín Medical & Veterinary Microbiology Medical Genetics, Viruses Microbes at the host surface Sara K Linden and Michael A McGuckin Chikungunya fever: A review of a re-emerging mosquito-borne infectious disease and the current status Po-Ying Chia, Mary Mah-Lee Ng and Justin Jang Hann Chu Cortactin, an oncoprotein targeted by pathogens during infection E. Nieto-Pelegrín, E. Meiler, and N. Martínez-Quiles Apparent impact of enteric pathogens in drinking water and implications for the relentless saga of HIV/AIDS in South Africa MNB Momba, E Madoroba and CL Obi Campylobacter concisus: an emerging pathogen of the gastrointestinal tract Taghrid Istivan, Peter Ward and Peter Coloe Regulation of the LEE-pathogenicity island in attaching and effacing bacteria C. Lara-Ochoa, R. Oropeza and A. Huerta-Saquero VII

8 The genomes of Group B Streptococcus and the relationship to pathogenesis of disease H. H. L. Ma and M. Ip Incidence of fungalysin and subtilisin virulence genes in dermatophytes A. Lemsaddek, L. Chambel and R. Tenreiro Bacteria and yeast normal microbiota from respiratory tract and genital area of bottlenose dolphins (Tursiops truncatus) R. Avalos-Téllez, F. Suárez-Güemes, E. M. Carrillo-Casas, and R. Hernández-Castro Lactobacilli and other lactic acid-related bacteria in the mucosal proximal gastrointestinal tract of pigs: a review of ecology for two derivative approaches for isolation of novel species Mareike Kraatz Leptospirosis: an important zoonotic diseasesis Angeliki R. Burriel Experiences in Mexico of the vaccination in bovines and goats, with Brucella abortus RB51 strain E. Herrera-López, F. Suárez-Güemes, B. Arellano Reynoso, E.G. Palomares-Resendiz, R. Hernández-Castro, and E. Díaz Aparicio Bacterial biofilms: Importance in animal diseases F. Aguilar-Romero, N.A. Pérez-Romero, E.Díaz-Aparicio and R. Hernández-Castro Identification and Characterization of Novel Adhesins in Leptospira M. V. Atzingen, G. M. Cerqueira, M. L. Vieira, R. Oliveira, T. R. Oliveira, R. F. Domingos, A. T. Barros, R. S. Mendes and Ana L. T. O. Nascimento The role of viruses in encephalitides of unknown origin in dogs A. M. Amude, A. F. Alfieri, A. A. Alfieri Clinical courses and neurological signs of canine distemper virus infection in dogs A. M. Amude, A. F. Alfieri, A. A. Alfieri Non-conventional neuropathological manifestations of canine distemper virus infection in dogs A. M. Amude, A. F. Alfieri, A. A. Alfieri Canine distemper virus and multiple sclerosis: A real or an anecdotal association? A. M. Amude, A. F. Alfieri, A. A. Alfieri Deciphering the infection biology of avian pathogenic Escherichia coli: role of experimental infection models Francis Dziva Vibrio parahaemolyticus Strain ATCC Contains Two Transcriptionally Active 16S rrna Genes and neither degrades preferentially in starvation N. González-Escalona, C.X. Moreno, J. Romero and R.T. Espejo Differentiation and typing of Vibrio parahaemolyticus and V. vulnificus strains by variability in their 16S-23S rrna intergenic spacer regions Narjol González-Escalona VIII

9 Aichi virus in Alberta, Canada: a one year examination of human diarrheic stool samples by RT-PCR A. Houde, E. Poitras, D. Leblanc, P. Ward, G.D. Inglis, V.F. Boras, F. Loisy-Hamon and B. Lebeau Preferential Assortment of Rearranged NSP3 Gene in the Genetic Background of Simian Rotavirus SA11 Mohammed Mahbub Alam, Nobumichi Kobayashi, Masaho Ishino, and Souvik Ghosh Laboratory surveillance of co-circulating respiratory viruses and enteroviruses during the 2009 H1N1 influenza pandemic J. Tan, and M.P. Carlos IX

10 VOL. 2 Introduction... XVII Biofilms, Quorum Sensing Manipulating bacterial cell fate: key role of surface-sensing and signal transduction C. Dorel Sugarcane and Grapevine Endophytic Bacteria: Isolation, Detection of Quorum Sensing Signals and Identification by 16S v3 rdna Sequence Analysis A. O. Hudson, N. H. Ahmad, R. Van Buren, and M. A. Savka Microbial biofilms: case reviews of bacterial and fungal pathogens persisting on biomaterials and environmental substrata Ian R. Cooper Multiple approaches to control dental unit water line biofilm contamination Iram Liaqat and Anjum Nasim Sabri A Lactobacillus-derived biosurfactant inhibits biofilm formation of human pathogenic Candida albicans biofilm producers L. Fracchia, M. Cavallo, G. Allegrone, and M.G. Martinotti The Perfect Adhesive Stephen A. Klotz and Peter N. Lipke Advances in industrial biofilm control with micro-nanotechnology Ferreira C., Pereira A. M., Melo L. F, Simões M Biofilms: An Evolving and Universal Evasive Strategy of Bacterial Pathogens S.Sekhar, M.Ohri, A.Chakraborti Atomic force microscopy and optical microscopy: suitable tools for the study of the initial stages of biofilm formation Patricia Schilardi, Carolina Diaz, Constanza Flores, Florencia Alvarez, and Mónica Fernández Lorenzo de Mele Industrial & Food Microbiology, Biodegradation, Production of chemicals and pharmaceuticals Optimization of cell mass production of the probiotic strain Lactococcus lactis in batch and fed-bach culture in pilot scale levels N. Elmarzugi, H. El Enshasy, R. Abd Malek, Z. Othman, M. R. Sarmidi, and R. Abdel Aziz Current trends of ß-galactosidase research and application S. Sheik Asraf and P. Gunasekaran X

11 Characteristics and potential use of b-glucosidases from Zygomycetes Judit Krisch, Miklós Takó, Tamás Papp, Csaba Vágvölgyi Lignocellulose as raw material in fermentation processes S. I. Mussatto, and J. A. Teixeira Improvement of wine organoleptic characteristics by non-saccharomyces yeasts L. Mendoza and M.E. Farías Strategies for the enhancement of malolactic fermentation in the new climate conditions J.Vila-Crespo, J.M. Rodriguez-Nogales, E. Fernández-Fernández, and M.C. Hernanz- Moral Yarrowia lipolytica: an industrial workhorse M.A.Z. Coelho, P.F.F. Amaral and I. Belo Characterization of a thermostable α-glucosidase from Geobacillus thermodenitrificans F84a A. C. Cihan, B. Ozcan, N. Tekin, and C. Cokmus Microbial Fuel Cells (MFCs) - a novel source of energy for new millennium L. Veeranjaneya Reddy, S. Pradeep Kumar Young-Jung Wee Botulinum neurotoxin: a deadly protease with applications to human medicine K.M. Land and L.W. Cheng Progresses on the knowledge about the ecological function and structure of the protists community in activated sludge wastewater treatment plants L. Arregui, B. Pérez-Uz, H. Salvadó and S. Serrano Decisionmaking in the development of a biocatalytic route for resolution of S- naproxen from screening to scale-up LH Steenkamp and D Brady Microbial ecology of anaerobic reactors for treatment of alcohol industry wastewaters: a review B.K. Đnce, N. Ayman-Öz, G. Türker, Ş. Çelikkol, O. Đnce Biocatalytic potential of thermophilic bacteria and actinomycetes B.A. Kikani, R.J. Shukla, and S.P. Singh Nitroreductases: Enzymes with Environmental, Biotechnological and Clinical Importance Iuri Marques de Oliveira, Diego Bonatto and João Antonio Pêgas Henriques Strain and process development for citric acid production from glycerol-containing waste of biodiesel manufacture S.V. Kamzolova, S.G. Anastassiadis, A.R. Fatyhkova, N.P. Golovchenko, and I.G. Morgunov Design and Development of Microbial Fuel cells Sourish Karmakar, Kanika Kundu and Subir Kundu XI

12 Competition Between Spontaneous And Commercial Yeasts In Winemaking: Study Of Possible Factors Involved N. Barrajón, M. Arévalo-Villena, J. Úbeda, and A. Briones Exploring the biodiversity of a wine region: Saccharomyces yeasts associated with wineries and vineyards L. Mercado, M. Combina Chitin deacetylase: A comprehensive account on its role in nature and its biotechnological applications V. Ghormade, S. Kulkarni, N. Doiphode, P.R. Rajamohanan and M.V. Deshpande Microbial diversity within the phyllosphere of different vegetable species Baoguo Zhang, Zhihui Bai, Daniel Hoefel, Xiaoyi Wang, Ling Zhang and Zuming Li Microbiological Control of Mycotoxins: Present Status and Future Concerns K.R.N. Reddy, N.I. Farhana, B. Salleh and C.A.F. Oliveira Are consumers aware of the risks related to Biogenic Amines in food? P. Russo, G. Spano, M.P. Arena, V. Capozzi, D. Fiocco, F. Grieco and L. Beneduce Biology of Escherichia coli O157:H7 in human health and food safety with emphasis on sublethal injury and detection Amanda L. Robinson and John L. McKillip Molecular Mechanisms of Stress Resistance in Lactococcus lactis William M. Smith, Gary A. Dykes, Aijaz H. Soomro and Mark S. Turner Dualistic aspects of Enterococcus spp. in foods Bruna Carrer Gomes, Bernadette Dora Gombossy de Melo Franco and Elaine Cristina Pereira De Martinis Nutritional potential of biomass and metabolites from filamentous fungi J.A. Takahashi and S.A. Carvalho Potential for manipulating the polysaccharide content of shiitake mushrooms D. Brauer, T.E. Kimmons, M. Phillips, and D.E. Brauer Fresh-cut fruits preservation: current status and emerging technologies M.R. Corbo, B. Speranza, D. Campaniello, D. D Amato, and M. Sinigaglia Functional nutrition and psycho-intellectual possibilities of man: things in common E.I. Tkachenko, Yu.P. Uspenskiy, Yu.A. Fominykh Advantages of traditional lactic acid bacteria fermentation of food in Africa PK Chelule, MP Mokoena and N Gqaleni Update in bread fermentation by lactic acid bacteria G. Rollán, C.L. Gerez, A. M. Dallagnol, M.I. Torino and G. Font Coliform bacteria in fresh vegetables: from cultivated lands to consumers M.P. Falomir, D. Gozalbo, and H. Rico The problem of Listeriosis and ready-to-eat products: prevalence and persistence V. Garrido, A.I.Vitas and I. García-Jalón XII

13 Potential links between irrigation water quality and microbiological quality of food in subsistence farming in KwaZulu-Natal, South Africa Megan E. Gemmell and Stefan Schmidt Production of Lactobacillus salivarius, a new probiotic strain isolated from human breast milk, in semi-industrial scale and studies on its functional characterization Roslinda Abd Malek, Sallehhuddin bin Hamdan, Hesham A. El Enshasy Nor Zalina Othman, Noor Azwani Zainol, Mohamad R. Sarmidi, Ramlan, A. Aziz Microbial safety of raw milk cheeses traditionally made at a ph below 4.7 and with other hurdles limiting pathogens growth F. Perez Pacheco, and A. Bucio Galindo Effect of modified atmosphere packaging on the growth/survival of Yersinia enterocolitica and natural flora on fresh poultry sausage C.A. Conte-Junior, B.T. Macedo, M.M. Lopes, R.M. Franco, M.Q. Freitas, M. Fernandez and S.B. Mano S-layers in lactobacilli: structural characteristics and putative role in surface and probiotic properties of whole bacteria P. Mobili, E. Gerbino, E. E. Tymczyszyn, and A. Gómez-Zavaglia Enhanced biodegradation of 4-chlorophenol using biosurfactant in an activated sludge A. Uysal and A. Turkman Removal of Ca 2+ impurities from brine by marine cyanobacteria from Gujarat coast of India for the production of Industrial grade salt S Mishra, PK Ghosh, MR Gandhi, AM Bhatt and SA Chauhan Preliminary characterization of an estuarine, benzoate-utilizing Vibrio sp. isolated from Durban Harbour, South Africa Karis Moxley and Stefan Schmidt Microorganisms capable to degrade organochlorine pesticides María S. Fuentes, Claudia S. Benimeli, Sergio A. Cuozzo, Juliana M. Saez and María J. Amoroso Microbial cold-active α-amylases: From fundamentals to recent developments Mohammed Kuddus, and Roohi Microbial biodegradation potential of hydrocarbons evaluated by colorimetric technique: a case study E. D. Bidoia, R. N. Montagnolli and P. R. M. Lopes Current Research Trends of Microbiological Leaching for Metal Recovery from Industrial Wastes Debaraj Mishra, Young-Ha Rhee Adaptative response and degradation of quaternary ammonium compounds by Pseudomonas putida A ATCC Gloria I. Lucchesi, Andrés S. Liffourrena, Paola S. Boeris and Mario A. Salvano Microbiological reduction of hexavalent molybdenum to molybdenum blue Shukor M.Y. and M.A Syed XIII

14 Comparison of marine and terrestrial carbazole-degrading bacteria Rintaro Maeda, Yoshihiko Ito, Kenichi Iwata and Toshio Omori Green bean coffee as nutrient source for pesticide degrading-bacteria B.E. Barragán-Huerta, and R. Rodríguez-Vázquez Removal of organic load from wastewater by using Datura innoxia Mill. N. Vaillant-Gaveau, F. Monnet, H. Sallanon, A. Coudret, and A. Hitmi The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7 Slavica Ilić, Sandra Konstantinović, Vlada B. Veljković, Dragiša S. Savić and Gordana Đ. Gojgić-Cvijović Mycofabrication, mechanistic aspect and Multifunctionality of Metal Nanoparticles - Where are we? And where should we go? Mahendra Rai, Alka Yadav, Aniket Gade Third generation biofuels from microalgae Giuliano Dragone, Bruno Fernandes, António A. Vicente, and José A. Teixeira Biosurfactants from marine bacterial isolates R.Thavasi, S. Jayalakshmi and I.M. Banat Citric acid production by yeasts: Fermentation conditions, process optimization and strain improvement Seda Karasu Yalcin, M. Tijen Bozdemir, Z. Yesim Ozbas Advances in butanol production by clostridia Y. Tashiro, and K. Sonomoto Bacterially Produced Polyhydroxyalkanoate (PHA): Converting Renewable Resources into Bioplastics Jiun-Yee Chee, Sugama-Salim Yoga, Nyok-Sean Lau, Siew-Chen Ling, Raeid M. M. Abed and Kumar Sudesh Microbial production of biodegradable polymers and their role in cardiac stent development P. Basnett and I. Roy Methods and Techniques Design of experiments: a powerful tool in food microbiology A. Bevilacqua, M.R. Corbo and M. Sinigaglia Specific and Sensitive Multiplex PCR Method for Detecting Salmonellae and Shigellae in Mayonnaise E. Villalobo Introduction of PapilloCheck, a microarray-based assay for the detection and genotyping of HPV, into the clinical laboratory D.A. Pilger, T.T. Moreira, D.M. Leinberger, and V.V. Cantarelli XIV

15 Sequencing a bacterial genome: an overview J. Duan, J.J. Heikkila, B.R. Glick Predictive microbiology and table olives F.N. Arroyo-López, J. Bautista-Gallego, F. Rodríguez-Gómez, and A. Garrido-Fernández Disposable electrochemical biosensors in microbiology O. Domínguez-Renedo, M.A. Alonso-Lomillo, M.J. Arcos-Martínez Unraveling activities by functional-based approaches using metagenomic libraries from dry and rain forest soils in Puerto Rico José M. Cruz, Manuel A. Ortega, Jean C. Cruz, Pedro Ondina, Rossivette Santiago, and Carlos Ríos-Velázquez Comparison of methods for the extraction of bacterial DNA from human faecal samples for analysis by real-time PCR E.A Nelson, E.A Palombo, and S.R Knowles Ca-alginate hydrogel rheological changes caused by yeast cell growth dynamics I. Pajic-Lijakovic, M. Plavsic, V. Nedovic, B. Bugarski The development of a robust industrial crystallography program for the bacterial 50S ribosomal subunit B.A. Maguire Tracking down microbial communities via fatty acids analysis: analytical strategy for solid organic samples M. Lores M. Gómez-Brandón and J. Domínguez Application of Amplicon Length Polymorphism to differentiate amongst closely related strains of bacteria W. C. Rice Genetic tools for desiccation- and radiation-tolerant cyanobacteria of the genus Chroococcidiopsis D. Billi PCR on slide: advantages in environmental and clinical microbiology J.-C. Avarre, E. Masseret, M. Alunni-Fabbroni, P. Hartmann, W. Mann, N. Häfner, M. Dürst, C. Woudstra, P. Fach, G. Cerovic, M. Rattier, A. Le bras, R. Melizzi, J.-L. Grabias and C. Weisbuch Preparation and analysis of environmental DNA: optimisation of techniques for phylogenetic analysis of ATAD sludge A.V. Piterina and J.T. Pembroke Use of immunohistochemistry and molecular assays such as RT-PCR for precise post mortem diagnosis of distemper-related encephalitis A. M. Amude, A. F. Alfieri, A. A. Alfieri Microbial Biodiversity Investigation techniques S. Saman, P. Slattery and S. Saman XV

16 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology A. Méndez-Vilas (Ed.) Challenges in Environmental Monitoring of Pathogens: Case Study in Mycobacterium avium N. Radomski, R. Moilleron, F. S. Lucas, and J. O. Falkinham III A modified cell extraction method to access microbial community structure in soil samples by phospholipid fatty acid analysis J. Malave-Orengo, S. E. Borglin, T. C. Hazen and C. Rios-Velazquez Generation of large insert metagenomic libraries using indirect DNA extraction methods from benthic and ephemeral tropical hypersaline microbial mats I. Torres-Zapata, A. Gonzalez-Montalvo, C. Castro-Ruiz and C. Rios-Velazquez Protein- protein interaction mutants to study structure and assembly of cellular machineries: its application to the prokaryotic divisome L. Grenga Fourier tansform infrared (FT-IR) spectroscopy: A rapid tool for detection and analysis of foodborne pathogenic bacteria R. Davis and L.J. Mauer UVA-LED Air Disinfection M. Gadelmoula, K. Mawatari, M. Yamato, M. Akutagawa, Y. Kinouchi, and Akira Takahashi Electron microscopy techniques and energy dispersive X-ray applied to determine the sorption of lead in Paracoccus sp. DE2007 Massimiliano Baratelli, Juan Maldonado, Isabel Esteve, Antoni Solé and Elia Diestra Others Transport of dicarboxylates in Saccharomyces cerevisiae D.A. Aliverdieva, D.M. Mamaev, L.S. Lagutina, and D.I. Bondarenko XVI FORMATEX 2010