Others ph conductivity measurement module ph and conductivity measurement in 96 deep well plates

Transcription

1 Others ph conductivity measurement module ph and conductivity measurement in 96 deep well plates Technical Reference EU Input Process Output 96 well DWP with 2 ml sample Plate is transported by the RoMa to the loading position Storage 48 well plate with storage solution and calibration buffers Automated calibration of the ph electrodes Test the calibration of the conductivity electrodes Simultaneous measurement of ph (4 wells) and conductivity (4wells) Measurement of 12 rows in straight plate orientation (A1) Turn the plate with the RoMa (regrip position) Measurement of 12 rows rotated plate orientation (H12) Washing and drying of electrodes Storage of electrodes in suitable storage solution Text files with calibration and calibration test results for the ph and conductivity electrodes Text files with ph and conductivity and temperature values for each well

2 System Overview # Type of module Brand, model 1. ph Electrodes Mettler Toledo InLab Micro ph probe without temperature sensor 2. Conductivity Electrodes Mettler-Toledo InLab 751-4mm conductivity probe with temperature sensor 3. Loading position Tecan 4. Electrode dryer Tecan 5. Calibration and Storage Plate Axygen 48 Well Clear V-Bottom 5mL Polypropylene Rectangular Well Deep Well Plate 6. Wash Station Tecan 7. Measurement devices Mettler Toledo, SevenMulti ph/conductivity meter 8. System Liquid Tubing Tecan 9. Waste Tubing Tecan 10. Support Frame Tecan The whole module can be integrated on the right side of a Freedom EVO 100, 150 or 200. An eccentric RoMa is required for loading the plates as well as a regrip station on the Freedom EVO worktable to ensure loading in straight (A1) or rotated (H12) orientation in order to measure all wells. Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 2/6

3 Highlights: TIG parts Parallel measurement with 4 ph- and 4 conductivity probes The minimal immersion depths of the electrodes recommended by the manufacturer are 5 mm for ph and 8-15 mm for conductivity electrodes. Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 3/6

4 Wash station for ph and conductivity electrodes The active wash station washes every tip in a separate well with a duct from below. Wash power can be set in the macro. The overflow water is passively running through the waste tubing. Electrode dryer The 8 channel electrode dryer is working as an air knife with filtered compressed air while the electrodes are moving up through the air flow. Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 4/6

5 Highlights: Freedom EVOware driver Store Macro To avoid drying out of the ph electrode, it can be stored in a suitable ph solution in the first column of the 48 well storage/calibration plate. This position is approached when choosing the lower position. The upper position can be used for exchanging the storage/calibration plate or for long term storage (ph electrodes have to be capped with liquid filled caps provided by the manufacturer) Measure Macro Wash macro For cleaning the electrodes before or after a calibration measure or store step, the wash macro can be used. The parameters are the same as in the measure, calibrate ph or test conductivity macro. It is recommended to wash the electrodes before each calibration or measurement. Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 5/6

6 Calibrate ph Macro The ph calibration is carried out according to the setting of the measurement device. The buffer is detected manually and the user can define the acceptable borders for the slope between two buffers. If desired, the results will be prompted after the calibration and the user can decide if the calibration shall be saved or not. If the data is not shown the calibration will be automatically be saved if the slopes are inside the allowed range. The values are anyway saved in an output file. Test Conductivity Calibration The conductivity calibration test is carried like a measurement, but buffers are stored in the 48 well storage and calibration plate. The user defines the acceptable borders for expected value for each buffer to be measured. If desired, the results will be prompted after the measurement and the user can decide if the process shall proceed or not. The values are anyway saved in an output file. Scientific instrumentation; not for use in human, clinical or diagnostic procedure. All systems or products are tailor-made one-offs that were exclusively developed on customer demand. All displayed pictures and images originate from development-, test- or installation phases and do not claim to represent the final installation at customer site. All mentioned trademarks are protected by law. In general, the trademarks and designs referenced herein are trademarks, or registered trademarks, of Tecan Group Ltd., Männedorf, Switzerland. A complete list may be found at Product names and company names that are not contained in the list but are noted herein may be the trademarks of their respective owners. 2014, Tecan Trading AG, Switzerland, all rights reserved. For disclaimer and trademarks please visit Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 6/6