Microbiological Analysis

Transcription

1 Microbiological Microbiological 117

2 Gridded Membrane Filters from Cellulose Nitrate (Cellulose Ester) acc. to ISO Norms, Sterile and Individually Packed, for Colony Counts Membrane Filters Microbiological These membrane filters are in accordance with the following norms: ISO 7704, ISO , ISO 8199, ISO and EN Sterile, individually packed filters have long become standard for routine microbiological quality control because of the user benefits they offer. They are pre-sterilized and ready-to-use and save preparatory time. As they are individually packed, they avoid the possibility of contamination of remaining filters in opened packs and conform with GLP, having filter identification and lot number printed on each individual envelope. The increasing demand on these filters required the construction of a new packaging machine with ultra-modern stamping. Each membrane is checked to ensure it is not damaged in any way, is positioned correctly with no slippage under the edge seal, has perfect grid printing and is free of particles. Each envelope is checked for readable lettering. Quality control par excellence! The membrane filters All membranes are made of cellulose nitrate, a material which assures effective retention with high flow rates and optimum colony growth. The printed grid with a size of mm makes the counting easier, especially for higher bacteria counts and for microcolonies, but does not influence the growth. The various filter colors allow the best contrast to the colonies (see membrane filter types, chosen for the nutrient pad sets, page 122). Hydrophobic edge membranes are used mainly in the sterility testing of solutions containing antibiotics. Specifications for gridded filter types, as for filter type 113 (page 120). SMART filter dispenser manually operated for membrane tapes. An automatic dispenser will be available starting 2 nd half of

3 Order numbers for sterile, individually packed membrane filters Type 114, white with black grid, for bacteriological research In packs of ACN 1.20 µm ACN 0.80 µm ACN 0.65 µm ACN 0.45 µm ACN 0.20 µm In packs of ACR 1.20 µm ACR 0.80 µm ACR 0.45 µm In packs of ACN 1.20 µm ACN 0.80 µm ACN 0.65 µm ACN 0.45 µm In packs of ACR 0.45 µm ACR 1.20 µm Membrane Filters Microbiological Type 130, grey (black after wetting) with white grid, for the detection of yeasts and moulds In packs of ACN 0.80 µm ACN 0.65 µm ACN 0.45 µm In packs of ACR 0.80 µm ACR 0.45 µm In packs of ACN 0.80 µm ACN 0.65 µm ACN 0.45 µm In packs of ACR 0.65 µm ACR 0.45 µm Type 131, white with black grid and pink-colored hydrophobic edge, for microbiological examination of products that contain inhibitors With 3 mm edge, in packs of ACN 0.45 µm ACN 0.20 µm, in packs of ACN 0.45 µm ACN 0.20 µm With 6 mm edge, in packs of HEN 0.45 µm Order number for SMART filter dispenser

4 Membrane Filters Microbiological Type 138, green with dark-green grid, for bacteriological research In packs of ACN 0.45 µm In packs of ACR 0.45 µm In packs of ACN 0.45 µm In packs of ACR 0.45 µm Type 139, white with green grid, for the detection of E.coli, coliform bacteria and other types of bacteria In packs of ACN 1.20 µm ACN 0.45 µm In packs of ACN 0.65 µm ACR 0.45 µm In packs of ACN 0.45 µm In packs of ACR 0.45 µm Type 113, white, no grid, for prefilter holder 16807, page 137, in packs of ACN 8 µm, in packs of ACN 8 µm Dispenserfilter SCM *, contains 3 boxes of 100 membranes white black SCM 0.45 µm grey white SCM 0.45 µm green dark green SCM 0.45 µm * 50 mm are available on request Membrane tapes packed without protective papers. They will be available starting 2 nd half of Order numbers for gridded membrane filters, non-sterile, not individually packed, for particle testing, microscopy and microbiology Type 114, white with black grid 25 mm diameter, in packs of N 1.20 µm N 0.80 µm N 0.45 µm 37 mm diameter, in packs of N 0.80 µm, in packs of N 1.20 µm N 0.80 µm N 0.65 µm N 0.45 µm, in packs of N 1.20 µm N 0.80 µm N 0.45 µm 120

5 Type 130, grey (black after wetting) with white grid 25 mm grid, in packs of N 0.45 µm, in packs of N 0.80 µm N 0.65 µm N 0.45 µm, in packs of N 0.80 µm N 0.65 µm N 0.45 µm Type 131, white with black grid and 3 mm pink-colored, hydrophobic edge 25 mm diameter, in packs of N 0.45 µm N 0.20 µm, in packs of N 8.00 µm N 0.45 µm N 0.20 µm, in packs of N 8.00 µm N 0.45 µm N 0.20 µm Membrane Filters Microbiological Type 131, with 6 mm pink-colored, hydrophobic edge, in packs of HCN 0.45 µm HCN 0.20 µm Type 138, green with dark-green grid In packs of N 0.45 µm In packs of R 0.45 µm In packs of N 0.45 µm In packs of R 0.45 µm Type 139, white with green grid In packs of N 0.45 µm In packs of R 0.45 µm In packs of N 0.45 µm In packs of R 0.45 µm Type 135, cellulose acetate, white, with hydrophobic edge, in packs of ACN HCN N ACN N, in packs of ACN 121

6 Dehydrated Media Pads in Petri Dishes, with Matching Membrane Filters for Economical, Time-saving Microbiological Quality Control Media Microbiological Sartorius nutrient pad sets have been used successfully in combination with the membrane filter method for more than 20 years now. They ease and simplify a lot of microbiological procedures, due to their practical handling. One nutrient pad set consists of a 50 mm pad, evenly impregnated with nutrient media, packed dry and sterile in a petri dish, plus a suitable membrane filter, tested for colony growth, sterile and individually packed. Directly after wetting with sterile, demineralised water, the nutrient pad set is ready to use. Benefits for the user Economy. No time-consuming and labour-intensive preparation of the nutrient media (sterilization, cleaning, etc.). Easy handling. Nutrient pad sets can also be used in laboratories without comprehensive microbiological equipment. Consistent quality. During the production, each nutrient pad set batch is compared with the corresponding agar medium, in order to guarantee consistent quality and reproducible results. Trouble-free storage. nutrient pad sets can be stored at room temperature in a warehouse, between 18 and 24 months depending on the type. Order numbers for nutrient pad sets in petri dishes Nutrient pad set Determinaton Order type Detection*: number**: Azide Enterococci (1) N Bismut sulfite Salmonellae (1) N Caso Colony count (1) N Cetrimide Pseudomonas aeruginosa (2) N Chapman Staphylococci (2) N Chromocult E. coli and coliforms (7) N ECD E. coli (2) N Endo E. coli and coliforms (2) N Glucose-Tryptone Mesophilic bacteria and N thermophilic spore formers (2) Lysine Wild yeasts (3) N Malt extract Yeasts and moulds (6) N McConkey Enterobacteria (2) N M-FC E. coli and coliforms (2) N Orange serum Acid-tolerant microbes (1) N Orange serum (ph 3.2) Acid-tolerant microbes (1) N R2A Colony count (1) N Sabouraud Yeasts and moulds (3) N Schaufus-Pottinger Yeasts and moulds (4) N Schaufus-Pottinger Yeasts and moulds (5) N Schaufus-Pottinger Yeasts and moulds (6) N Schaufus-Pottinger Yeasts and moulds (3) N Standard Colony count (1) N Standard TTC Colony count (1) N 122

7 Order numbers for nutrient pad sets in petri dishes Nutrient pad set Determinaton Order type Detection* number** Teepol E. coli und coliforms (2) N Tergitol TTC E. coli und coliforms (2) N TGE Colony count (1) N Tomato juice Lactic acid bacteria, special Leuconostoc oenos (1) N VLB-S7-S Beer spoiling bacteria (2) N Weman Leuconostoc mesenteroides (1) N Wallerstein Lactic acid bacteria and other beverage spoilers N Wort Yeasts and moulds (3) N Wort (ph 4,4) Yeasts and moulds (3) N Yeast extract Colony count (1) N Media Microbiological Special brochure available on request. Order no. SM-4017-e * A pack contains 100 sterile nutrient pad sets, each of them individually packed in a petri dish, and 100 sterile, individually packed membrane filters. The membrane filters are selected for optimum growth together with the corresponding nutrient media. The supplied membrane filter type is listed within brackets: (1) = green with dark green grid, 0.45 µm pore size (2) = white with green grid, 0.45 µm pore size (3) = grey (black after wetting) with white grid, 0.65 µm pore size (4) = white with green grid, 0.65 µm pore size (5) = white with green grid, 1.2 µm pore size (6) = grey (black after wetting) with white grid, 0.8 µm pore size (7) = white with black grid, 0.45 µm pore size ** Diameter of the membrane filter, 47 mm. Order number for nutrient pad set with 50 mm membrane filter as above, but -047N replaced by -050N. Some nutrient pad sets are available in polyethylene bags, without petri dishes. Each pack containts 25 bags with 2 nutrient pad sets and 2 membrane filters each, all sterile. Order numbers with 50 membrane filters: Endo, K. Standard TTC, K. Tergitol TTC, K. Wort, K. Order numbers with 47 mm membrane filters as for 50 mm but with -047 instead of